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FARMERS’ PERCEPTION OF CLIMATE CHANGE AND PARTHENIUM WEED DISTRIBUTION IN DISTRICT SWABI, KHYBER PAKHTUNKHWA 开伯尔-普赫图赫瓦省斯瓦比地区农民对气候变化的感知与parthenium weed分布
Pub Date : 2022-10-05 DOI: 10.28941/pjwsr.v28i3.887
H. Khan
Climate change is the principal concern of present day agriculturists, environmental scientists and ecologists. Climate change and its negative impacts is still not fully understood and the concept is still criticized for its validity. The present research work consists of two phases. The first phase analyzed the farmers’ perception of climate change and its likely impact on weeds distribution by conducting a formal survey and in the 2nd phase a laboratory experiment was conducted on Parthenium weed selected from study area in Weed Science Department, The University of Agriculture, Peshawar to correlate the findings of both phasesin July-September, 2016. The field survey was carried out in Jan-May, 2016 in Swabi district, Khyber Pakhtunkhwa- Pakistan. 100 farmers from the study area were randomly interviewed through a questionnaire comprised of all relevant parameters to assess their perception of climate change and its consequences.  Most of the farmers (61%) believed in climate change and its negative consequences and complained of lower efficiency of their farming in the changed scenario. They had noticed climatic variations, for the last 20 years, in the form of  summers extension, winters flexion, unexpected and changed pattern of rainfalls (58% responses for increase) with summer rainfalls earlier (68%) and winter rainfalls later (35%), severe and destructive floods (75%), storms or strong winds (77%), hailstorms (67%) and higher  insects/ pests attack causing harm to crops (68%), livestock/ animal (64%) and humans. The changed scenario also posed difficulties to farmers in managing agricultural activities like abundance of weed species (62% farmers strongly agreed) especially the invasive ones e.g. Parthenium hysterophorus, Silybam marianum and Rumex crispus etc. They noticed increase in weed species (78%), frequency (69%) and interaction (78%) among native and invasive weeds. These weeds had aggressive (75%) and uncontrollable growth (68%), out competed the crops (82%), and survived longer (55%). From the field study Parthenium weed was concluded (82%) as the most responsive invasive weed to climate change and was selected for lab study. The experiment was laid out in Completely Randomized Design (CRD) with 4 replications. The treatments comprised of 5 different temperature regimes of 15, 20, 25, 30 and 35 °C in controlled situation in an incubator. The analysis illustrated that high germination % (34.50%) of Parthenium weed seeds occurred at higher temperature (30°C) than optimum (25°C) which was 29.25%, following the same pattern for the growth which remained more (19.75) and (17.45) at higher temperatures of (30°C) and (35°C) respectively than optimum (16.95). The conclusion of lab experiment was that the rise in temperature is favoring the growth of Parthenium weed. This weed is reported in the surveyed area to be highly aggressive and competitive for the indigenous flora expanding its domain in the area.
气候变化是当今农业学家、环境科学家和生态学家最关心的问题。气候变化及其负面影响仍未被完全理解,这一概念的有效性仍受到批评。目前的研究工作分为两个阶段。第一阶段通过正式调查分析农民对气候变化的感知及其对杂草分布的可能影响;第二阶段于2016年7 - 9月在白沙瓦农业大学杂草科学系选取研究区域的Parthenium杂草进行实验室实验,以将两阶段的研究结果联系起来。实地调查于2016年1月至5月在巴基斯坦开伯尔-普赫图赫瓦省斯瓦比地区进行,来自研究地区的100名农民通过包含所有相关参数的问卷随机采访,以评估他们对气候变化及其后果的看法。大多数农民(61%)相信气候变化及其负面影响,并抱怨在变化的情况下他们的农业效率较低。他们注意到气候变化,在过去的20年里,以夏季延长,冬季弯曲,意外和变化的降雨模式(58%响应增加),夏季降雨提前(68%)和冬季降雨推迟(35%),严重和破坏性的洪水(75%),风暴或强风(77%),冰雹(67%)和更高的昆虫/害虫袭击造成伤害农作物(68%),牲畜/动物(64%)和人类。变化的情景也给农民管理农业活动带来了困难,如杂草种类丰富(62%的农民强烈同意),特别是侵入性杂草,如Parthenium hysterophorus、Silybam marianum和Rumex crispus等。他们注意到,杂草种类(78%)、频率(69%)和本地杂草与入侵杂草之间的相互作用(78%)都有所增加。这些杂草具有侵略性(75%)和不可控生长(68%),与作物竞争(82%),存活时间更长(55%)。在野外研究中,Parthenium weed(82%)被认为是对气候变化最敏感的入侵杂草,并被选中进行实验室研究。试验采用完全随机设计(CRD),设4个重复。在恒温箱中控制15、20、25、30和35 °C的5种不同温度。结果表明,在温度(30°C)高于最适温度(25°C)的条件下,Parthenium weed种子的发芽率为34.50%,高于最适温度(29.25%);在温度(30°C)和温度(35°C)下,Parthenium weed种子的发芽率分别为19.75%和17.45%,高于最适温度(16.95%)。室内实验结果表明,温度的升高有利于Parthenium weed的生长。据报道,在调查地区,这种杂草对本地植物群具有高度的侵略性和竞争性,并扩大了其在该地区的领域。
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引用次数: 1
RECALCITRANCE POTENTIAL OF WHEAT HYBRIDS AND LOCAL CULTIVAR AGAINST YELLOW RUST DISEASE AND WEEDS DENSITY UNDER CLIMATIC CONDITION OF PESHAWAR, KHYBER PAKHTUNKHWA 白沙瓦、开伯尔-普赫图赫瓦气候条件下小麦杂交种和地方品种对黄锈病的抗性潜力及杂草密度
Pub Date : 2022-09-30 DOI: 10.28941/pjwsr.v28i3.1054
Muhammad Mehran Anjum, M. Arif, Bismillah Khan, N. Ali, G. R. Khan
Wheat cultivars can be severely affected by new race of leaf and stripe rust which possessing Yr27 resistance gene in Pakistan and worldwide during previous four years. Thus, great importance could be given to develop new wheat cultivar that could resist leaf and stripe rust diseases. Yellow rust disease is caused by certain pathogens like basidiomycete fungus Puccina striiformis Westend f. species tritici Eriks, it is one of the most devastating foliar diseases of wheat in Pakistan and worldwide. Two years experiments were conducted in Agronomy Research Farm, The University of Agriculture Peshawar, Pakistan to evaluate the potential response of wheat hybrid lines (18A-1 and 18A-2) in comparison with local check (Ghaneemat-2016) against yellow rust disease.Results exhibited that wheat hybrid 18A-1 depicted resistant (R) with coefficient of infection (CI) of 0.1. Hybrid wheat 18A-2 showed host reaction (10MR) with coefficient of infection (4.0). Contrary to this, local wheat variety (Ghaneemat-2016) showed moderate susceptibility (30MS) type of host reaction with 24 CI value during season 2018-19. A moderate resistant (MR) type of host reaction with CI (16.0) was observed for local variety (Ghaneemat-2016) during season 2019-20. Among wheat hybrids 18A-2 depicted no visible infection while 18A-1 exposed resistant 1R type of host reaction with CI (0.2) during 2019-20.Chinese wheat hybrids have the high potential to resist against yellow rust as compared with local check variety. Higher weeds density, fresh and dry weeds density increased by increasing irrigation frequencyfor local check Ghaneemat-2016 when sown early (15th November). Conclusively, from the study it is statedin wheat breeding program, identification of molecular markers facilitates the marker assisted selection of the resistance genes against yellow rust diseases, furthermore molecular markers should be used for marker assisted permitted that efficiently screening the large population.
近4年来,巴基斯坦和世界范围内的小麦品种受到了具有Yr27抗性基因的叶锈病和条锈病新变种的严重影响。因此,培育抗叶锈病和条锈病的小麦新品种具有重要意义。黄锈病是由担子真菌引起的,是巴基斯坦乃至世界范围内最具破坏性的小麦叶面病害之一。在巴基斯坦白沙瓦农业大学农学研究农场进行了为期两年的试验,以评估小麦杂交种(18A-1和18A-2)与当地对照(Ghaneemat-2016)对黄锈病的潜在反应。结果表明,杂交小麦18A-1的抗侵染系数(CI)为0.1。杂交小麦18A-2表现出寄主反应(10MR),侵染系数(4.0)。与此相反,当地小麦品种(Ghaneemat-2016)在2018-19季表现出中度(30MS)型寄主反应,CI值为24。在2019-20季节,本地品种(Ghaneemat-2016)观察到中度抗性(MR)型宿主反应,CI为16.0。在小麦杂交种中,18A-2未表现出明显的感染,而18A-1在2019- 2020年期间暴露出抗性1R型宿主与CI(0.2)的反应。与地方对照品种相比,中国小麦杂交种具有较高的抗黄锈潜力。杂草密度更高,在当地检查Ghaneemat-2016播种早期(11月15日),通过增加灌溉频率增加新鲜和干杂草密度。研究表明,在小麦育种规划中,分子标记的鉴定有助于标记辅助筛选抗黄锈病基因,因此分子标记应用于标记辅助筛选,以有效地筛选大群体。
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引用次数: 0
A CASE STUDY OF NUTRITIONAL STATUS OF CHERRY (Prunus serotina), ORCHARD SOILS AND FRUIT QUALITY ATTRIBUTES OF BALTISTAN REGION 以巴尔蒂斯坦地区樱桃营养状况、果园土壤及果实品质属性为例
Pub Date : 2022-09-30 DOI: 10.28941/pjwsr.v28i3.1053
Javaid Hassan, Anwar Zaman, Navid Iqbal, H. Ali, Amir Muhammad khan, Z. Hussain, Aziz Sheikh
This study had been conducted in north eastern area of Pakistan, Baltistan region constituting the larger portion of Kashmir region. Which is one of the moist zone of the western Himalayas. While growing fruit point of view the land is suitable for fruit like apples, plum, cherry and apricot production.  Climatically Baltistan region soils have recognized as best for quality cherry production. However, limited information is available about the existing soil fertility status and fruit quality of cherry orchards. Soil fertility evaluation is a key factor towards sustainable fruit production of cherry orchard soils. Improvement of soil productivity and fruit quality depends on the knowledge of the soil fertility. Therefore, study was designed to investigate the soil fertility status of cherry orchards of District Skardu, Kharmang, Shigar, and Ganache. Soil samples were evaluated for pH, EC, Organic matter, Texture, N, P, K, and micronutrients. While leaf and fruits samples were analyzed for N, P, K and pH, TSS, TA, Ascorbic acid, and Sugar contents respectively. Results revealed that the soil samples were slightly alkaline and non-saline sandy loam and sandy clay loam texture with adequate organic matter ranging from 1.19-2.15%, Low to marginal (NO3-N), low extractable P, and marginal to adequate K with high micronutrients concentrations (Cu, Zn, Fe, and Mn). While leaf samples showed marginal to high N, low P, and K and sufficient to high micronutrients. The parameters of fruit samples were in the optimum range with a yield of 5-50 Kgplant-1.
这项研究是在巴基斯坦东北部地区进行的,巴尔蒂斯坦地区占克什米尔地区的大部分。它是西喜马拉雅山脉的湿润地带之一。从种植水果的角度来看,这片土地适合苹果、李子、樱桃和杏等水果的生产。气候巴尔蒂斯坦地区的土壤被认为是最好的优质樱桃生产。然而,关于樱桃园现有土壤肥力状况和果实品质的资料有限。土壤肥力评价是决定樱桃园土壤果实可持续生产的关键因素。提高土壤生产力和果实品质取决于对土壤肥力的认识。因此,本研究旨在调查斯卡尔都、哈尔曼、日喀尔和甘纳许地区樱桃园的土壤肥力状况。评估土壤样品的pH、EC、有机质、质地、N、P、K和微量元素。叶片和果实分别测定N、P、K、pH、TSS、TA、抗坏血酸和糖的含量。结果表明:土壤样品为微碱性和非盐碱性砂质壤土和砂质粘土壤土,有机质含量在1.19 ~ 2.15%之间,低至边际(NO3-N),低可提取P,低至边际充足K,微量元素(Cu、Zn、Fe和Mn)浓度高。叶片样品对高氮、低磷、低钾表现出边缘性,对高微量元素表现出充足性。果实样品的各项参数均在最佳范围内,产量为5 ~ 50 Kgplant-1。
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引用次数: 1
Phosphate Solubilizing Bacteria (PSB) Improve Phosphorus Availability from Various Sources in Alkaline Soils 磷酸盐增溶菌(PSB)提高了碱性土壤中各种来源磷的有效性
Pub Date : 2022-09-30 DOI: 10.28941/pjwsr.v28i3.1068
Anwar Zaman, H. Ali, Javaid Hassan, A. Khan, Navid Iqbal, F. M. Khan, Ijaz Ahmad, M. Fawad, Irfan Ullah, N. Ali
Phosphorus (P) is one of the major essential macronutrients for plants, applied to soil in the form of phosphatic fertilizers and organic manures. The application of PSB to soils can replace or partially reduce use of inorganic P fertilizers and could be one of the cost effective, highly efficient, and sustainable approaches to conserve P resources. The aim of the study was to evaluate the role of PSB in phosphorus (P) availability from organic, mineral and natural sources. For this purpose, a laboratory incubation experiment was conducted at the University of Agriculture Peshawar.  The experiment was consisting of four sources of P [poultry manure (PM), farm yard manure (FYM), rock phosphate (RP) and single supper phosphate (SSP)] for obtaining 45 mg P2O5 kg-1 and two types of inoculation (control and PSB) arranged in two factorial complete randomized design with three replications. Resultantly, PSB inoculation significantly improved. P availability at all incubation interval except day zero. A 3.4%, 5.4%, 12% and 16 % increased were observed in P as compared to control, at day 7, 14, 28 and 56 respectively.  Similarly, significant differences were observed in P release for P sources at all incubation intervals. Initially higher P release was observed for SSP but at day 14 onward PM and FYM proved to be the best P releasable sources as compared to mineral P sources. RP released the lowest P at all incubation intervals however it performed statistically at par to SSP at 56 days.  The significant interactive effect of P sources and PSB at day 28 and 56 demonstrated that soil receiving P from organic sources inoculated with PSB released higher P than other treatments combination. Thus, it is suggested that P must be applied from organic P sources (PM and FYM) along with PSB inoculation for better P nutrition and crop yield.
磷(P)是植物必需的主要常量养分之一,以磷肥和有机肥的形式施用于土壤中。在土壤中施用PSB可以替代或部分减少无机磷肥的使用,是节约磷资源的一种经济、高效、可持续的方法。本研究的目的是评价PSB在有机、矿物和天然来源的磷(P)有效性中的作用。为此目的,在白沙瓦农业大学进行了实验室孵化实验。试验采用禽粪(PM)、农家肥(FYM)、磷矿粉(RP)和单超磷(SSP) 4种磷源,以获得45 mg P2O5 kg-1, 2种接种方式(对照和PSB),采用2因子完全随机设计,3个重复。结果,PSB接种率显著提高。P在除第0天以外的所有潜伏期内的可用性。第7天、第14天、第28天和第56天,P与对照相比分别提高了3.4%、5.4%、12%和16%。同样,在所有孵育时间内,磷源的磷释放量也有显著差异。起初,SSP的磷释放量较高,但在第14天以后,PM和FYM被证明是最好的磷释放源,与矿物磷源相比。RP在所有孵育间隔释放的P最低,但在统计上与SSP在56天的表现相当。磷源与PSB在第28天和第56天的显著交互作用表明,接种PSB的有机源磷土壤释放的磷量高于其他处理组合。因此,建议在接种PSB的同时,从有机磷源(PM和FYM)中施用磷,以获得更好的磷营养和作物产量。
{"title":"Phosphate Solubilizing Bacteria (PSB) Improve Phosphorus Availability from Various Sources in Alkaline Soils","authors":"Anwar Zaman, H. Ali, Javaid Hassan, A. Khan, Navid Iqbal, F. M. Khan, Ijaz Ahmad, M. Fawad, Irfan Ullah, N. Ali","doi":"10.28941/pjwsr.v28i3.1068","DOIUrl":"https://doi.org/10.28941/pjwsr.v28i3.1068","url":null,"abstract":"Phosphorus (P) is one of the major essential macronutrients for plants, applied to soil in the form of phosphatic fertilizers and organic manures. The application of PSB to soils can replace or partially reduce use of inorganic P fertilizers and could be one of the cost effective, highly efficient, and sustainable approaches to conserve P resources. The aim of the study was to evaluate the role of PSB in phosphorus (P) availability from organic, mineral and natural sources. For this purpose, a laboratory incubation experiment was conducted at the University of Agriculture Peshawar.  The experiment was consisting of four sources of P [poultry manure (PM), farm yard manure (FYM), rock phosphate (RP) and single supper phosphate (SSP)] for obtaining 45 mg P2O5 kg-1 and two types of inoculation (control and PSB) arranged in two factorial complete randomized design with three replications. Resultantly, PSB inoculation significantly improved. P availability at all incubation interval except day zero. A 3.4%, 5.4%, 12% and 16 % increased were observed in P as compared to control, at day 7, 14, 28 and 56 respectively.  Similarly, significant differences were observed in P release for P sources at all incubation intervals. Initially higher P release was observed for SSP but at day 14 onward PM and FYM proved to be the best P releasable sources as compared to mineral P sources. RP released the lowest P at all incubation intervals however it performed statistically at par to SSP at 56 days.  The significant interactive effect of P sources and PSB at day 28 and 56 demonstrated that soil receiving P from organic sources inoculated with PSB released higher P than other treatments combination. Thus, it is suggested that P must be applied from organic P sources (PM and FYM) along with PSB inoculation for better P nutrition and crop yield.","PeriodicalId":17469,"journal":{"name":"JOURNAL OF WEED SCIENCE RESEARCH","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2022-09-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"84309359","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
INTERACTION OF COMPATIBLE RHIZOBIAL STRAINS WITH LENTIL VARIETIES IMPROVES SEED YIELD AND QUALITY 亲和根瘤菌与小扁豆品种的互作提高了种子产量和品质
Pub Date : 2022-09-30 DOI: 10.28941/pjwsr.v28i3.1067
Syed Inzimam Ul Haq
Lentil yield is very poor in rainfed areas of pothwar due to non-availability of improved/recommended lentil varieties compatible to some specific rhizobial inoculants along the site-specific production technology. Therefore, to study the effect of inoculating lentil varieties with different Rhizobium strains on lentil yield and quality, pot experiments were conducted in the greenhouse of University Research Farm, Koont, Chakwal Road, Rawalpindi during 2019-20 and 2020-21. Five lentil varieties (NIAB Masoor-2016, NIAB Masoor-2006, Markaz-09, Punjab Masoor-09, Punjab-11) and five rhizobial strains (RSâ‚’ (control), RS-1, RS-2, RS-3, RS-4) were used. The experiment was laid out in completely randomized design (CRD) with two factorial arrangement and four replications. All the agronomic recommendations were applied as such. Data regarding crop growth, yield and nodulation were collected during the experimentation. Maximum Germination % was recorded.
由于无法获得与某些特定根瘤菌接种剂相容的改良/推荐的扁豆品种,因此在波特瓦旱地扁豆产量非常低。因此,为研究不同根瘤菌菌株接种扁豆品种对扁豆产量和品质的影响,于2019- 2020年和2020-21年在拉瓦尔品第Chakwal路Koont大学研究农场温室进行了盆栽试验。选用5个小扁豆品种(NIAB Masoor-2016、NIAB Masoor-2006、Markaz-09、Punjab Masoor-09、Punjab-11)和5个根瘤菌(rs、对照、RS-1、RS-2、RS-3、RS-4)。试验采用完全随机设计(CRD),两因子排列,4个重复。所有的农艺建议都是这样应用的。在试验期间收集了作物生长、产量和结瘤的数据。记录最高发芽率。
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引用次数: 0
IDENTIFICATIONS OF PHENOLIC COMPOUNDS AND ALLELOPATHIC EFFECT OF Glycyrrhiza glabra ON GERMINATION AND SEEDLING GROWTH Phalaris minor 甘草酚类化合物的鉴定及其对小蝴蝶兰萌发和幼苗生长的化感作用
Pub Date : 2022-09-30 DOI: 10.28941/pjwsr.v28i3.1045
B. Khan, R. Maqbool, M. Nadeem, I. Anwar, M. Javaid, A. Jabbar, M. Amin
The present study was planned to identify the phenolic compounds and allelopathic effect of Glycyrrhiza glabra on germination and seedling growth Phalaris minor at Weed Science Laboratory, Department of Agronomy, University of Agriculture, Faisalabad during winter 2018. The experiments were laid out under a completely randomized design (CRD), having three replicates. In this study seven different concentrations of G. glabra aqueous extracts (0%, 0.25%, 0.5%, 10%, 20%, 40%, and 80%) applied on winter weed P.  minor.  Data on regarding germination percentage (%), mean Germination time (days), time to 50% germination, root length (mm), shoot length (mm), root fresh weight (mg), shoot fresh weight was recorded following standard procedures. Data were analyzed using Fishers Analysis of Variance. The differences among treatments were separated using a least significant difference test (LSD). Results of experiment revealed that maximum germination percentage (%), Mean Germination time (Days), Time to 50% germination and minimum, root length (mm), shoot length (mm), root fresh weight (mg), shoot fresh weight of p. minor was recorded at 80 % concentration of G.  glabra.  From the present study, it is concluded that there are phenolic compounds in the wood extract of Glycyrrhiza glabra which cause allelopathic effects on P. minor.
本研究计划于2018年冬季在费萨拉巴德农业大学农学系杂草科学实验室鉴定光甘草对小蝴蝶花发芽和幼苗生长的酚类化合物和化感作用。试验采用完全随机设计(CRD),设3个重复。本研究采用7种不同浓度的光天麻水提液(0%、0.25%、0.5%、10%、20%、40%和80%)对冬杂草小天麻进行处理。按照标准程序记录发芽率(%)、平均发芽时间(天)、萌发至50%时间、根长(mm)、茎长(mm)、根鲜重(mg)、茎鲜重等数据。数据分析采用fisher方差分析。采用最小显著性差异检验(LSD)分离处理间差异。试验结果表明,在80%浓度下,小青苗的最大发芽率(%)、平均发芽时间(天)、至50%萌发时间和最小根长(mm)、茎长(mm)、根鲜重(mg)、茎鲜重均有显著差异。本研究认为,光甘草木提取物中含有对小蠊有化感作用的酚类化合物。
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引用次数: 1
RESPONSE OF Citrullus colocynthis L. TO SINGLE AND COMBINED STRESSES OF SALINITY (NaCl) AND HEAVY METAL (NiCl 2 ) 甜瓜(Citrullus colocynthis L.)对盐(NaCl)和重金属(NiCl 2)单一和联合胁迫的响应
Pub Date : 2022-09-30 DOI: 10.28941/pjwsr.v28i3.1057
H. Javeed
Salinity and heavy metal stresses are great hindrances to germination and the early developmentof plants. Nickel (Ni) is an indispensable micronutrient for plants at low while toxic in highconcentrations. Citrullus colocynth L. is a medicinal cucurbit weed in desert and agriculturalareas. Germination and early development of seedlings determine the future of the plant. Thecontemporary target of the study is to appraise the influence of single and combined stresses byvarying concentrations of NaCl and NiCl 2 on germination and seedlings of C. colocynth.Germination percentage, radicle length, plumule length, fresh weight and dry weight of seedlingsof C. colocynth with three levels of salt (100mM, 200mM and 400mMNaCl), three levels ofheavy metal (50, 100, 200uMNiCl 2 ) and Nine combined levels(100mMNaCl +50uM NiCl 2 ),(200mMNaCl +50 uMNiCl 2 ) (400mMNaCl +50uM NiCl 2 ) (100mMNaCl +100uMNiCl 2 )(200mM NaCl +100uM NiCl 2 ) (400mMNaCl +100uMNiCl 2 ) (100mMNaCl +200uMNiCl 2 )(200mMNaCl +200 uMNiCl 2 ) (400mMNaCl +200uMNiCl 2 ) were studied. This experiment wasconceded out in a completely randomized design with four replications. The responses showedan adverse trend with increasing stress levels. Germination and seedling growth factors declinedunder high-stress levels of salt (400mM NaCl), high HM (200 uM NiCl 2 ), and combined highsalt or high HM but positively improved on lower salt(100mMNaCl), lower HM (50uMNiCl 2 )and combined lower salt with lower HM (100mMNaCl+50uMNiCl 2 ).
盐胁迫和重金属胁迫是植物萌发和早期发育的重要障碍。镍(Ni)是植物必需的微量营养素,浓度低而毒性高。Citrullus colocynth L.是沙漠和农业地区的药用瓜类杂草。幼苗的发芽和早期发育决定了植物的未来。本研究的当前目标是通过不同浓度的NaCl和NiCl 2来评估单一胁迫和组合胁迫对绿草甘蓝萌发和幼苗的影响。发芽率、胚根长、胚芽长、鲜重和干重的seedlingsof c .药西瓜三个级别的盐(100毫米,200毫米和400 mmnacl),三个层次ofheavy金属(50、100、200 uMNiCl 2)和9个组合水平(100 mmnacl + 50嗯NiCl 2) (200 mmnacl + 50 uMNiCl 2) (400 mmnacl + 50嗯NiCl 2) (100 mmnacl + 100 uMNiCl 2)(200毫米氯化钠+ 100嗯NiCl 2) (400 mmnacl + 100 uMNiCl 2) (100 mmnacl + 200 uMNiCl 2) (200 mmnacl + 200 uMNiCl 2) (400 mmnacl + 200 uMNiCl 2)进行了研究。这个实验是完全随机设计的,有四个重复。随着压力水平的增加,这些反应呈现出不利的趋势。在高盐(400mM NaCl)、高HM (200um NiCl 2)、高盐或高HM组合胁迫下,种子萌发因子和幼苗生长因子均下降,但在低盐(100mMNaCl)、低HM (50uMNiCl 2)和低盐与低HM (100mMNaCl+50uMNiCl 2)组合胁迫下萌发因子和幼苗生长因子均显著提高。
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引用次数: 0
Morphological responses of wheat and maize to plant derived smoke solution under salt stress 盐胁迫下小麦和玉米对植物源烟液的形态响应
Pub Date : 2022-09-30 DOI: 10.28941/pjwsr.v28i3.1069
S. Naveed, Farhana Hussain
One of the biggest threats to the increasing population of plants life is different stresses such as abiotic, comprising ultraviolet light and high temperature. Among these abiotic stresses, salt stress is considered as the most effective stress , causing 20% loss  of crop yeild all-over the world.It has been reported that plant derived smoke (PDS) contain some  biologically active  product or stimulating agents which help in stimlating seed germination and  increasing other morphological parameters of crops like plmule/radical length biomass of seedlings under salt stress conditions. To understand the role of salt stress and PDS solution, we performed invitro experiments to analyze the morphological aspects of wheat and Maize germination and post-germination growth under application of salt solutions. The Petri dishes culture experiment was conducted, whose design was completely randomized with five replicas and one control. Var Wadan and Azam varieties of wheat and Maize seeds, respectively, were used as test species and cymbopogan jawarancusa was used for smoke derivetion. Nine different concentration solutions of salt and smoke were applied on seeds to check the inhibitory and stimulatory potentials of salt and smoke, correspondingly. Different concentration solutions are 100mM NaCl, 150mMNacl, 200mM NaCl, 500ppm PDS, 1000ppm PDS, 2000ppm PDS, 100+500 (NaCl and PDS), 150+1000 (NaCl and PDS), 200+2000 (NaCl and PDS). Different parameters i.e., Germination%, plmule length (cm), Radical length (cm), Fresh weight (gm), Dry weight (gm), Moisture content (%) were selected for obtaining data. Basic method was used to record the data on the selected parameters. Result of experiment showed that Salinity affected all the selected parameters significantly. The significance of data was checked by using LSD test at alpha level 0.05, 0.01, 0.001.The morphological parameters of wheat and maize are badly effected under salt stress at 150 and 200 mM concentration. On the other hand, PDS significantly increased the germination rate and plmule, radical length was also increased due to some stimulatory agents. So under 2000 ppm PDS the seed germination rate and other morphological parameters are significantly enhanced. Another interesting thing, PDS at 1000, 2000 ppm concentration have the restoring ability of seeds germination under salt stress condition, suggesting that PDS  has a metigatory potential by improving morphological parameters under 150- and 200-mM salt stress.
对不断增长的植物种群的最大威胁之一是不同的胁迫,如非生物胁迫,包括紫外线和高温。在这些非生物胁迫中,盐胁迫被认为是最有效的胁迫,在世界范围内造成作物产量损失20%。据报道,植物源烟(PDS)含有一些生物活性产物或刺激物,有助于刺激种子萌发,增加作物在盐胁迫条件下幼苗的plmule/radical length生物量等其他形态参数。为了了解盐胁迫和PDS溶液的作用,本研究通过体外实验分析了盐溶液对小麦和玉米萌发和萌发后生长的影响。培养皿培养试验采用完全随机设计,5个重复,1个对照。以varwadan和Azam品种小麦和玉米种子分别为试验种,以cymbopogan jawarancusa为烟源。采用9种不同浓度的盐和烟分别施用于种子,考察盐和烟对种子的抑制和刺激作用。不同浓度的溶液有100mM NaCl、150mMNacl、200mM NaCl、500ppm PDS、1000ppm PDS、2000ppm PDS、100+500 (NaCl和PDS)、150+1000 (NaCl和PDS)、200+2000 (NaCl和PDS)。选取发芽率、叶柄长度(cm)、根茎长度(cm)、鲜重(gm)、干重(gm)、含水率(%)等参数进行数据采集。采用基本法记录所选参数的数据。试验结果表明,盐度对所选参数均有显著影响。采用LSD检验,α水平分别为0.05、0.01、0.001。150和200 mM浓度的盐胁迫对小麦和玉米的形态参数影响较大。另一方面,PDS处理显著提高了种子的发芽率和萌发率,并且由于某些刺激剂的作用,胚根长度也有所增加。因此,在2000 ppm PDS下,种子发芽率和其他形态参数显著提高。另一个有趣的现象是,在1000、2000 ppm浓度下,PDS具有恢复盐胁迫条件下种子萌发的能力,表明PDS具有通过改善150和200 mm盐胁迫下的形态参数来调节种子萌发的潜力。
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引用次数: 0
ANTIFUNGAL AND OTHER BIOACTIVE CONSTITUENTS IN ROOTS OF A HALOPHYTIC WEED Suaeda fruticosa 盐生植物suada fruticosa根中的抗真菌和其他生物活性成分
Pub Date : 2022-09-30 DOI: 10.28941/pjwsr.v28i3.1072
Nadeem Shad, A. Javaid, Q. Kanwal
Suaeda fruticosa Forssk. ex J. F. Gmelin is a halophytic weed of family Amaranthaceae (previously in Chenopodiaceae) that covers vast stretches of salty alluvial flats on drier sites and coastal belts in Pakistan. In this study, mature plants of S. fruticosa were collected from district D.G. Khan during May 2021. After shade drying, the roots were crushed thoroughly and 5 g material was soaked in methanol for 2 weeks. After filtration, GC-MS analysis of the extract was done that showed presence of 14 compounds in the extract. Major compounds in the root were n-hexadecanoic acid (16.70%), oleic acid (16.39%), dodecanoic acid (15.68%) and dihydroxymaleic acid (15.58%). Other compounds were erythritol  (6.31%), 10-octadecenoic acid, methyl ester (6.01%), tetradecanoic acid (4.78%), 1,2-ethanediamine, N-ethyl-N'-methyl- (3.46%), pentadecanoic acid, 14-methyl-, methyl ester (3.66%), 8,11-octadecadienoic acid, methyl ester (2.85%), 2,4,6-cycloheptatrien-1-one, 3,5-bis-trimethylsilyl (2.44%), 2-oxo-3-methyl-cis-perhydro-1,3-benzoxazine (2.24%), cyclotrisiloxane, hexamethyl- (2.13%), and 1-hexadecanol (1.73%). Compounds present in roots of S. fruticosa are known to possess antifungal, antibacterial, nematicidal, antimicrobial, anticancer, antidiabetic, anti-inflammatory, and antioxidant properties.
suedada fruticosa Forssk。Gmelin是苋科的一种盐生杂草(以前在Chenopodiaceae中),覆盖了巴基斯坦干旱地区和沿海地带的大片含盐冲积平原。在本研究中,于2021年5月在D.G. Khan地区采集了金丝桃的成熟植株。遮荫干燥后,将根彻底粉碎,5 g材料在甲醇中浸泡2周。过滤后,对提取物进行GC-MS分析,发现提取物中存在14种化合物。根中主要化合物为正十六酸(16.70%)、油酸(16.39%)、十二酸(15.68%)和二羟基马来酸(15.58%)。其他化合物依次为赤藓糖醇(6.31%)、10-十八烯酸甲酯(6.01%)、十四烷酸(4.78%)、1,2-乙二胺、n-乙基- n '-甲基-(3.46%)、五烷酸- 14-甲基-甲酯(3.66%)、8,11-十八烯二酸甲酯(2.85%)、2,4,6-环庚二烯-1- 1,3,5 -二-三甲基硅基(2.44%)、2-氧-3-甲基顺式过氢-1,3-苯并恶嗪(2.24%)、环三硅氧烷-六甲基-(2.13%)和1-十六烷醇(1.73%)。已知存在于金银花根中的化合物具有抗真菌、抗菌、杀线虫、抗菌、抗癌、抗糖尿病、抗炎和抗氧化特性。
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引用次数: 0
INVESTIGATING LOW DOSE OF WEED MEDIATED SILVER NANOPARTICLES AGAINST PATHOGENIC BACTERIA 研究低剂量杂草介导的银纳米颗粒对致病菌的作用
Pub Date : 2022-09-30 DOI: 10.28941/pjwsr.v28i3.1024
Rida Pasha, Syeda Hafsa Ali, Syeda Ayesha Ali, Rabail Mehak
Weeds are undesirable and unpleasant plants with robust growth. Around 18,000 weed species outcast native biodiversity and responsible for tremendous economic losses. Plants were used to treat numerous ailments since ancient times. Antimicrobial resistance (AMR) is an emerging global health problem, where approximately 700,000 people lose their life each year from AMR infections. Minimum inhibitory concentration of Biogenic silver nanoparticles against pathogenic bacterial strain has not been reported previously. This study aims to elucidate lowest biogenic Alkanna tinctoria mediated silver nanoparticles dose to treat bacterial infections. Qualitative phytochemical analysis performed using standard tests. The synthesized silver nanoparticles were characterized visually, UV–Vis spectroscopy and using X-ray diffraction. Minimum inhibitory concentration of biogenic silver nanoparticles was tested against gram positive (S. aureus) and gram-negative bacteria (E. coli). Alkanna tinctoria leave were positive for alkaloids, carbohydrate, saponins, amino acids, and fixed oil. The synthesized silver nanoparticles were in range of 60 to 73nm. Silver nanoparticles significantly affected E. coli as compared to S. aureus. The MIC for E. coli was recorded as 46.87 μg/mL and 93.75 μg/mL was effective against S.aureus. Search of naive plants as novel therapeutic agents along with nanotechnology can bring revolution in the field of drug development and medical sciences.
杂草是生长旺盛的不受欢迎和令人讨厌的植物。大约18000种杂草破坏了当地的生物多样性,造成了巨大的经济损失。自古以来,植物就被用来治疗许多疾病。抗菌素耐药性是一个新出现的全球卫生问题,每年约有70万人因抗菌素耐药性感染而丧生。生物源银纳米颗粒对病原菌的最低抑菌浓度尚未见报道。本研究旨在阐明生物源性最低的白碱介导的银纳米颗粒治疗细菌感染的剂量。采用标准试验进行定性植物化学分析。对合成的纳米银进行了视觉表征、uv - Vis光谱和x射线衍射。测定了生物源银纳米颗粒对革兰氏阳性菌(金黄色葡萄球菌)和革兰氏阴性菌(大肠杆菌)的最低抑制浓度。药叶生物碱、碳水化合物、皂苷、氨基酸和固定油检测阳性。合成的银纳米颗粒尺寸在60 ~ 73nm之间。与金黄色葡萄球菌相比,银纳米颗粒对大肠杆菌的影响显著。大肠杆菌的MIC值为46.87 μg/mL,金黄色葡萄球菌的MIC值为93.75 μg/mL。寻找原生植物作为新型治疗剂,再加上纳米技术,将给药物开发和医学领域带来革命性的变化。
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引用次数: 0
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