Pub Date : 2018-10-01DOI: 10.1016/S0254-6272(18)30905-1
Li Xiaohua , Li Haitao , Jin Lingyu , Chen Leixi , Niu Yingfeng , Guan Yanhong , Ma Xiaojun , Zhang Lixia
OBJECTIVE
To evaluate the anti-tumor activity of ethyl acetate fraction (EFA), extracted with ethanol from the root of “Dai-Bai-Jie” in A549 cancer cells and its underlying mechanism.
METHODS
“Dai-Bai-Jie” was extracted with 95% ethanol-aqueous (DBJ-1), 50% ethanol-aqueous (DBJ-2), and water (DBJ-3) by reflux method. 95% ethanol-aqueous extract was separated byethyl acetate (EFA) and n-butyl alcohol (DBJ-5), consecutively. The SRB method was used to evaluate the cytotoxic activity. Annexin V-FITC staining was applied to observe the apoptosis and analyze the cell cycle activated by EFA in A549 tumor cell. Western blot was used to detect the apoptosis/related proteins expressions. A549 tumor cellsbearing nude mice model was employed to measure the tumor volume, mice weight, and tumor inhibition ratio in order to verify the antitumor activity in vivo.
RESULTS
DBJ-1 and EFA showed better cytotoxic activity on A549 tumor cells with IC50 25 and 3.5 µg/mL, respectively. EFA can exhibit the proliferation, arrest cell cycle at G0/G1 phase, and induce apoptosis in A549 tumor cells in vitro. The mechanisms of apoptosis induced by EFA may be associated with decreasing Bcl-2 protein expression and increasing p53, Bax, Caspase-3, and Caspase-8 proteins expression. EFA also possessed significant anti-tumor efficacy in nude mice, and little toxicity was observed in the host.
CONCLUSION
EAF could induce A549 tumor cells apoptosis and G0/G1 cell cycle arrest. A549 tumor cells apoptosis induced by EAF may be associated with the decrease in the ratio of Bcl-2 and Bax mRNA levels, and increase in the expression of p53, Caspase-3, and Caspase-8 proteins.
{"title":"Ethyl acetate fraction in ethanol extract from root of “Dai-Bai-Jie” (Marsdenia tenacissima): anti-tumor activity in A549 cancer cells","authors":"Li Xiaohua , Li Haitao , Jin Lingyu , Chen Leixi , Niu Yingfeng , Guan Yanhong , Ma Xiaojun , Zhang Lixia","doi":"10.1016/S0254-6272(18)30905-1","DOIUrl":"10.1016/S0254-6272(18)30905-1","url":null,"abstract":"<div><h3>OBJECTIVE</h3><p>To evaluate the anti-tumor activity of ethyl acetate fraction (EFA), extracted with ethanol from the root of “Dai-Bai-Jie” in A549 cancer cells and its underlying mechanism.</p></div><div><h3>METHODS</h3><p>“Dai-Bai-Jie” was extracted with 95% ethanol-aqueous (DBJ-1), 50% ethanol-aqueous (DBJ-2), and water (DBJ-3) by reflux method. 95% ethanol-aqueous extract was separated byethyl acetate (EFA) and n-butyl alcohol (DBJ-5), consecutively. The SRB method was used to evaluate the cytotoxic activity. Annexin V-FITC staining was applied to observe the apoptosis and analyze the cell cycle activated by EFA in A549 tumor cell. Western blot was used to detect the apoptosis/related proteins expressions. A549 tumor cellsbearing nude mice model was employed to measure the tumor volume, mice weight, and tumor inhibition ratio in order to verify the antitumor activity <em>in vivo.</em></p></div><div><h3>RESULTS</h3><p>DBJ-1 and EFA showed better cytotoxic activity on A549 tumor cells with IC<sub>50</sub> 25 and 3.5 µg/mL, respectively. EFA can exhibit the proliferation, arrest cell cycle at G0/G1 phase, and induce apoptosis in A549 tumor cells <em>in vitro.</em> The mechanisms of apoptosis induced by EFA may be associated with decreasing Bcl-2 protein expression and increasing p53, Bax, Caspase-3, and Caspase-8 proteins expression. EFA also possessed significant anti-tumor efficacy in nude mice, and little toxicity was observed in the host.</p></div><div><h3>CONCLUSION</h3><p>EAF could induce A549 tumor cells apoptosis and G0/G1 cell cycle arrest. A549 tumor cells apoptosis induced by EAF may be associated with the decrease in the ratio of Bcl-2 and Bax mRNA levels, and increase in the expression of p53, Caspase-3, and Caspase-8 proteins.</p></div>","PeriodicalId":17513,"journal":{"name":"Journal of Traditional Chinese Medicine","volume":null,"pages":null},"PeriodicalIF":2.6,"publicationDate":"2018-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/S0254-6272(18)30905-1","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"44573953","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2018-10-01DOI: 10.1016/S0254-6272(18)30919-1
Zhang Jing , Xie Daojun , Guo Yanbing , Li Yajun , Li Liangyong , Han Hui , Zhang Juan , Chen Huaizhen , Wang Meixia
OBJECTIVE
To evaluate the efficacy and safety of gandouling plus sodium dimercaptosulphonate (DMPS) on neurological Wilson's disease (WD) in patients.
METHODS
We retrospectively evaluated the clinical records of 125 WD patients with neurological syndromes who were treated with gandouling plus sodium DMPS or DMPS used alone. All patients had a history of neurological deterioration during their diseases courses. The clinical efficacies, adverse reactions, and results of the various hematological and biochemical investigations were recorded for statistical analysis.
RESULTS
92.30% (60 patients) of the WD patients treated with the combined therapy experienced an improved or stable neurological condition paralleled by a significantly improved GAS score. Meanwhile, the WBC and PLT counts stabilized, liver function and renal function were improved or remained stable. The combined therapy also obviously promoted the 24-h urinary copper excretion. In particular, only 30.76% of the WD patients experienced mild adverse reactions, including neurological deterioration in 5 patients (7.69%), hepatic worsening in 1 subject (1.89%), which was less frequently than those in the control group treated with DMPS only.
CONCLUSION
Our findings indicate that the safety and efficacy of gandou-ling plus DMPS is superior to those of DMPS used alone in the WD patients with neurological symptoms.
{"title":"Evaluation of efficacy and safety of gandouling plus sodium dimercaptosulphonate in treatment of patients with neurological Wilson's disease from China","authors":"Zhang Jing , Xie Daojun , Guo Yanbing , Li Yajun , Li Liangyong , Han Hui , Zhang Juan , Chen Huaizhen , Wang Meixia","doi":"10.1016/S0254-6272(18)30919-1","DOIUrl":"10.1016/S0254-6272(18)30919-1","url":null,"abstract":"<div><h3>OBJECTIVE</h3><p>To evaluate the efficacy and safety of gandouling plus sodium dimercaptosulphonate (DMPS) on neurological Wilson's disease (WD) in patients.</p></div><div><h3>METHODS</h3><p>We retrospectively evaluated the clinical records of 125 WD patients with neurological syndromes who were treated with gandouling plus sodium DMPS or DMPS used alone. All patients had a history of neurological deterioration during their diseases courses. The clinical efficacies, adverse reactions, and results of the various hematological and biochemical investigations were recorded for statistical analysis.</p></div><div><h3>RESULTS</h3><p>92.30% (60 patients) of the WD patients treated with the combined therapy experienced an improved or stable neurological condition paralleled by a significantly improved GAS score. Meanwhile, the WBC and PLT counts stabilized, liver function and renal function were improved or remained stable. The combined therapy also obviously promoted the 24-h urinary copper excretion. In particular, only 30.76% of the WD patients experienced mild adverse reactions, including neurological deterioration in 5 patients (7.69%), hepatic worsening in 1 subject (1.89%), which was less frequently than those in the control group treated with DMPS only.</p></div><div><h3>CONCLUSION</h3><p>Our findings indicate that the safety and efficacy of gandou-ling plus DMPS is superior to those of DMPS used alone in the WD patients with neurological symptoms.</p></div>","PeriodicalId":17513,"journal":{"name":"Journal of Traditional Chinese Medicine","volume":null,"pages":null},"PeriodicalIF":2.6,"publicationDate":"2018-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/S0254-6272(18)30919-1","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"47402877","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2018-10-01DOI: 10.1016/S0254-6272(18)30910-5
Yi Yan , Zhao Yong , Li Chunying , Zhang Yushi , Bin Yang , Yuan Yalan , Wang Lianmei , Pan Chen , Liang Aihua
OBJECTIVE
To evaluate the safety of Oroxylumindicum(L.) Vent extract administered for 26 weeks in Wistar rats.
METHODS
Oroxylumindicum (L.) Vent extract was administrated to male and female rats by gavage once daily at doses of 54, 225, and450 mg • kg−1 • d−1. The rats were sacrificed after administration for13 weeks and 26 weeks. Part of the rats in each group were allowed to recover for 4 weeks after 26-week administration. Systematic examinations including haematology, urology, blood biochemistry and histomorphology were performed at the end of 13, 26 weeks of administration and 4 weeks of recovery.
RESULTS
No treatment related adverse effect shappened on rats'general status, body weight, food consumption, urinary index and histomorphology examination. Although during the administration, in some rats of extract's groups, the value of Red blood cell count (RBC), white blood cell counts (WBC), hemoglobin (HGB), haematocrit (HCT) and K iron were decreased, and biochemistry index, such as glucose (GLU), triglyceride (TG), alanine transaminase (ALT) and blood urea nitrogen(BUN) were increased, the above parameters were within the normal ranges and all returned to baseline after the drug stopping for 4 weeks.
CONCLUSION
The administration of Oroxylumindicum(L.) Vent extractat levers up to 450 mg/kg (equals to 75 times of clinical dose) is well tolerated-for both genders without significant toxicity within the administration duration in this study.
{"title":"Toxicity study of ethanol extract from Oroxylumindicum (L.) Vent in rats","authors":"Yi Yan , Zhao Yong , Li Chunying , Zhang Yushi , Bin Yang , Yuan Yalan , Wang Lianmei , Pan Chen , Liang Aihua","doi":"10.1016/S0254-6272(18)30910-5","DOIUrl":"10.1016/S0254-6272(18)30910-5","url":null,"abstract":"<div><h3>OBJECTIVE</h3><p>To evaluate the safety of Oroxylumindicum(L.) Vent extract administered for 26 weeks in Wistar rats.</p></div><div><h3>METHODS</h3><p>Oroxylumindicum (L.) Vent extract was administrated to male and female rats by gavage once daily at doses of 54, 225, and450 mg • kg<sup>−1</sup> • d<sup>−1</sup>. The rats were sacrificed after administration for13 weeks and 26 weeks. Part of the rats in each group were allowed to recover for 4 weeks after 26-week administration. Systematic examinations including haematology, urology, blood biochemistry and histomorphology were performed at the end of 13, 26 weeks of administration and 4 weeks of recovery.</p></div><div><h3>RESULTS</h3><p>No treatment related adverse effect shappened on rats'general status, body weight, food consumption, urinary index and histomorphology examination. Although during the administration, in some rats of extract's groups, the value of Red blood cell count (RBC), white blood cell counts (WBC), hemoglobin (HGB), haematocrit (HCT) and K iron were decreased, and biochemistry index, such as glucose (GLU), triglyceride (TG), alanine transaminase (ALT) and blood urea nitrogen(BUN) were increased, the above parameters were within the normal ranges and all returned to baseline after the drug stopping for 4 weeks.</p></div><div><h3>CONCLUSION</h3><p>The administration of Oroxylumindicum(L.) Vent extractat levers up to 450 mg/kg (equals to 75 times of clinical dose) is well tolerated-for both genders without significant toxicity within the administration duration in this study.</p></div>","PeriodicalId":17513,"journal":{"name":"Journal of Traditional Chinese Medicine","volume":null,"pages":null},"PeriodicalIF":2.6,"publicationDate":"2018-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/S0254-6272(18)30910-5","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"87936320","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2018-10-01DOI: 10.1016/S0254-6272(18)30908-7
Yang Min , Zhang Hongyan , Yue Rongzhao , Shi Qinchuan , Bian Yaoyao
OBJECTIVE
To investigate the analgesic effect of Gua Sha and its underlying mechanism in rats with noncompressive lumbar disk herniation induced by autologous nucleus pulposus.
METHODS
A rat model of noncompressive lumbar disk herniation was established and rats were randomly divided into model group, sham group, and Gua Sha group (24 in each group). Gua Sha was performed from the 5th day after the surgery, once every other day, 3 times for a course of treatment, and totally 3 courses. The thermal withdrawal latency was evaluated using the intelligent hot plate one day before the surgery, and on days 4 (the day before the treatment), 10 (the end of the first course), 16 (the end of the second course) and 22 (the end of the third course). On days 4, 10, 16 and 22, six rats in each group were picked randomly and their blood samples were drawn to assess the expression of interleukin-1β (IL-1β), interleukin-6 (IL-6) and tumor necrosis factor-alpha (TNF-α).
RESULTS
Compared to rats in the sham group, the application of nucleus pulposus to right L5 dorsal root ganglion induced prolonged thermal hyperalgesia, and up-regulated the expression of IL-1β, IL-6 and TNF-α in serum (P < 0.01). The therapy of Gua Sha attenuated thermal hyperalgesia potently, inhibited the expression of IL-1β, IL-6 and TNF-α in a time-dependent manner (P < 0.01). There were no significant differences in the thermal withdrawal latency and the expression of inflammatory cytokines between the sham and Gua Sha groups at the end of the treatment (P > 0.01).
CONCLUSION
The current study showed that Gua Sha might alleviate thermal hyperalgesia in rats with lumbar disc herniation induced by autologous nucleus pulposus via inhibiting the expression of proinflammatory cytokins.
{"title":"Gua Sha attenuates thermal hyperalgesia and decreases proinflammatory cytokine expression in serum in rats with lumbar disc herniation induced by autologous nucleus pulposus","authors":"Yang Min , Zhang Hongyan , Yue Rongzhao , Shi Qinchuan , Bian Yaoyao","doi":"10.1016/S0254-6272(18)30908-7","DOIUrl":"10.1016/S0254-6272(18)30908-7","url":null,"abstract":"<div><h3>OBJECTIVE</h3><p>To investigate the analgesic effect of Gua Sha and its underlying mechanism in rats with noncompressive lumbar disk herniation induced by autologous nucleus pulposus.</p></div><div><h3>METHODS</h3><p>A rat model of noncompressive lumbar disk herniation was established and rats were randomly divided into model group, sham group, and Gua Sha group (24 in each group). Gua Sha was performed from the 5th day after the surgery, once every other day, 3 times for a course of treatment, and totally 3 courses. The thermal withdrawal latency was evaluated using the intelligent hot plate one day before the surgery, and on days 4 (the day before the treatment), 10 (the end of the first course), 16 (the end of the second course) and 22 (the end of the third course). On days 4, 10, 16 and 22, six rats in each group were picked randomly and their blood samples were drawn to assess the expression of interleukin-1β (IL-1β), interleukin-6 (IL-6) and tumor necrosis factor-alpha (TNF-α).</p></div><div><h3>RESULTS</h3><p>Compared to rats in the sham group, the application of nucleus pulposus to right L5 dorsal root ganglion induced prolonged thermal hyperalgesia, and up-regulated the expression of IL-1β, IL-6 and TNF-α in serum (<em>P</em> < 0.01). The therapy of Gua Sha attenuated thermal hyperalgesia potently, inhibited the expression of IL-1β, IL-6 and TNF-α in a time-dependent manner (<em>P</em> < 0.01). There were no significant differences in the thermal withdrawal latency and the expression of inflammatory cytokines between the sham and Gua Sha groups at the end of the treatment (<em>P</em> > 0.01).</p></div><div><h3>CONCLUSION</h3><p>The current study showed that Gua Sha might alleviate thermal hyperalgesia in rats with lumbar disc herniation induced by autologous nucleus pulposus <em>via</em> inhibiting the expression of proinflammatory cytokins.</p></div>","PeriodicalId":17513,"journal":{"name":"Journal of Traditional Chinese Medicine","volume":null,"pages":null},"PeriodicalIF":2.6,"publicationDate":"2018-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/S0254-6272(18)30908-7","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"42748677","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2018-10-01DOI: 10.1016/S0254-6272(18)30912-9
Si Yuhao , Ma Yong , Guo Yang , Pan Yalan , Zheng Suyang , Xu Guihua , Da Weiwei , Xu Lili
PURPOSE
To observe the efficacy and safety of Shaoyang Xibi decoction (SYXBD) in patients with knee osteoarthritis (KOA), and to verify that the theory of “Shaoyang dominating bone” in Traditional Chinese Medicine (TCM) can be applied to KOA treatment.
METHODS
Participants were randomly allocated to two groups: SYXBD (treatment group, n = 66) and Meloxicam (control group, n = 66). Western Ontario and McMaster Universities Osteoarthritis Index (WOMAC) and 36-Item Short Form Health Survey (SF-36) were used to assess efficacy before the treatment and 8 weeks after the treatment.
RESULTS
Baseline data before the treatment between the two groups were similar. The WOMAC scores significantly decreased and the SF-36 scores significantly increased after 8- week treatment in both groups compared with before the treatment (P < 0.05). SYXBD significantly decreased pain scores (P < 0.001), physical function scores (P < 0.001) and the total scores (P < 0.001) in WOMAC compared to Meloxicam. SYXBD significantly improved physical function (P = 0.021), bodily pain (P = 0.002) and general health (P = 0.014), with no significant difference in role emotional (P = 0.053), role physical (P = 0.517), vitality (P = 0.241), social function (P = 0.712) and mental health (P = 0.800) in SF-36 compared to Meloxicam. No adverse events were reported in the treatment group while 13 adverse events happened in the control group during the study.
CONCLUSION
SYXBD, prepared based on the theory of “Shaoyang dominating bone”, has a better curative efficay and safety in patients with KOA compared with Meloxicam. The TCM theory of “Shaoyang dominating bone” may be useful in KOA treatment.
{"title":"Efficacy and safety of Shaoyang Xibi decoction in patients with knee osteoarthritis: a multi-center, single-blind, randomized controlled trial","authors":"Si Yuhao , Ma Yong , Guo Yang , Pan Yalan , Zheng Suyang , Xu Guihua , Da Weiwei , Xu Lili","doi":"10.1016/S0254-6272(18)30912-9","DOIUrl":"10.1016/S0254-6272(18)30912-9","url":null,"abstract":"<div><h3>PURPOSE</h3><p>To observe the efficacy and safety of Shaoyang Xibi decoction (SYXBD) in patients with knee osteoarthritis (KOA), and to verify that the theory of “Shaoyang dominating bone” in Traditional Chinese Medicine (TCM) can be applied to KOA treatment.</p></div><div><h3>METHODS</h3><p>Participants were randomly allocated to two groups: SYXBD (treatment group, <em>n</em> = 66) and Meloxicam (control group, <em>n</em> = 66). Western Ontario and McMaster Universities Osteoarthritis Index (WOMAC) and 36-Item Short Form Health Survey (SF-36) were used to assess efficacy before the treatment and 8 weeks after the treatment.</p></div><div><h3>RESULTS</h3><p>Baseline data before the treatment between the two groups were similar. The WOMAC scores significantly decreased and the SF-36 scores significantly increased after 8- week treatment in both groups compared with before the treatment (<em>P</em> < 0.05). SYXBD significantly decreased pain scores (<em>P</em> < 0.001), physical function scores (<em>P</em> < 0.001) and the total scores (<em>P</em> < 0.001) in WOMAC compared to Meloxicam. SYXBD significantly improved physical function (<em>P</em> = 0.021), bodily pain (<em>P</em> = 0.002) and general health (<em>P</em> = 0.014), with no significant difference in role emotional (<em>P</em> = 0.053), role physical (<em>P</em> = 0.517), vitality (<em>P</em> = 0.241), social function (<em>P</em> = 0.712) and mental health (<em>P</em> = 0.800) in SF-36 compared to Meloxicam. No adverse events were reported in the treatment group while 13 adverse events happened in the control group during the study.</p></div><div><h3>CONCLUSION</h3><p>SYXBD, prepared based on the theory of “Shaoyang dominating bone”, has a better curative efficay and safety in patients with KOA compared with Meloxicam. The TCM theory of “Shaoyang dominating bone” may be useful in KOA treatment.</p></div>","PeriodicalId":17513,"journal":{"name":"Journal of Traditional Chinese Medicine","volume":null,"pages":null},"PeriodicalIF":2.6,"publicationDate":"2018-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/S0254-6272(18)30912-9","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"45102853","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2018-10-01DOI: 10.1016/S0254-6272(18)30916-6
Ni Jing , Wang Bin , Wang Fuzhuang , Zhou Hongwei , Shi Huaxin , Zhang Ninan , Zhao Hong , Xie Qi
OBJECTIVE
To evaluate the effectiveness and safety of auricular acupoint bloodletting in treatment of insomnia
METHODS
Participants (n = 60) with insomnia were randomized into two groups to receive treatment of auricular acupoint bloodletting: low frequency group, 1 times/week for five weeks (n = 30); high frequency group, 2times/week for two weeks (n = 30). The following outcomes were measured blindly at baseline, after first treatment, 4 weeks, and 8 weeks: Pittsburgh sleep quality index scale (PSQI).
RESULTS
The groups were balanced at baseline for insomnia and demographic characteristics. There were no significant differences between the groups in terms of any of the outcomes, at the first follow-up time point. However, the therapeutic effect of LFG (once per week) is obviously lower than that of HFG (twice per week). In addition, there was no significant difference in the side effects between the two groups.
CONCLUSION
The treatment of insomnia with different frequencies of auricular acupoint bloodletting is effective and has less side effects. More reasonable treatment frequencies are worth further study.
{"title":"Effectiveness and safety of auricular acupoint bloodletting in treatment of insomnia: an assessor-blinded pilot randomized controlled trial","authors":"Ni Jing , Wang Bin , Wang Fuzhuang , Zhou Hongwei , Shi Huaxin , Zhang Ninan , Zhao Hong , Xie Qi","doi":"10.1016/S0254-6272(18)30916-6","DOIUrl":"10.1016/S0254-6272(18)30916-6","url":null,"abstract":"<div><h3>OBJECTIVE</h3><p>To evaluate the effectiveness and safety of auricular acupoint bloodletting in treatment of insomnia</p></div><div><h3>METHODS</h3><p>Participants (<em>n</em> = 60) with insomnia were randomized into two groups to receive treatment of auricular acupoint bloodletting: low frequency group, 1 times/week for five weeks (<em>n</em> = 30); high frequency group, 2times/week for two weeks (<em>n</em> = 30). The following outcomes were measured blindly at baseline, after first treatment, 4 weeks, and 8 weeks: Pittsburgh sleep quality index scale (PSQI).</p></div><div><h3>RESULTS</h3><p>The groups were balanced at baseline for insomnia and demographic characteristics. There were no significant differences between the groups in terms of any of the outcomes, at the first follow-up time point. However, the therapeutic effect of LFG (once per week) is obviously lower than that of HFG (twice per week). In addition, there was no significant difference in the side effects between the two groups.</p></div><div><h3>CONCLUSION</h3><p>The treatment of insomnia with different frequencies of auricular acupoint bloodletting is effective and has less side effects. More reasonable treatment frequencies are worth further study.</p></div>","PeriodicalId":17513,"journal":{"name":"Journal of Traditional Chinese Medicine","volume":null,"pages":null},"PeriodicalIF":2.6,"publicationDate":"2018-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/S0254-6272(18)30916-6","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"46016844","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2018-10-01DOI: 10.1016/S0254-6272(18)30911-7
Gao Wulin , Dai Guohua , Zhang Tong , Bi Dongxue , Liu Chunhua , Shi Xiaojing , Zhao Fang , Zhao Chen
OBJECTIVE
To investigate the effects of tonifying Qi and activating blood circulation (SQABC), a method in Traditional Chinese Medicine (TCM), on end-point events in patients with myocardial infarction (MI) in this retrospective cohort study.
METHODS
Clinical data were obtained from the medical records of patients with acute MI (AMI), both during hospitalization and follow-up, and included general demographic information (age, gender, and contact information), TCM regimens used, and end-point events.
RESULTS
A total of 1596 patients with AMI were enrolled to this study, but data of only 1210 cases are accessible till follow-up. We classified the patients based on the exposure levels of SQABC. When comparing the results between all exposure and non-exposure groups, significant differences were identified, both during hospitalization and follow-ups. During hospitalization, cardiac death (4.40% vs 21.55%, P < 0.05) and cardiac shock (3.04% vs 11.62%, P < 0.05) were significantly lower in the exposure group than the non-exposure group. Similarly, during the follow-up, cardiac death (12.04% vs 20.49%, P < 0.05), acute heart failure (7.27% vs 11.81%, P < 0.05), composite endpoint of reinfarction and stroke (9.11% vs 15.28%, P < 0.05), and rehospitalization due to angina (25.49% vs 34.38%, P < 0.05) were significantly lower in the exposure group than the non-exposure group.
CONCLUSION
Our findings suggest that SQABC can significantly benefits the subjects in the management of high-risk AMI in them.
目的通过回顾性队列研究，探讨补气活血法(SQABC)对心肌梗死(MI)患者终点事件的影响。方法从急性心肌梗死(AMI)患者住院和随访期间的医疗记录中获取临床资料，包括一般人口学信息(年龄、性别、联系方式)、使用的中医方案和终点事件。结果本研究共纳入1596例AMI患者，但截至随访仅有1210例患者资料可查。我们根据SQABC的暴露水平对患者进行分类。当比较所有暴露组和非暴露组之间的结果时，在住院和随访期间都发现了显著差异。住院期间心源性死亡(4.40% vs 21.55%， P <0.05)和心源性休克(3.04% vs 11.62%， P <0.05)，暴露组显著低于未暴露组。同样，在随访期间，心源性死亡(12.04% vs 20.49%， P <0.05)，急性心力衰竭(7.27% vs 11.81%， P <0.05)，再梗死和卒中的复合终点(9.11% vs 15.28%， P <0.05)，心绞痛再住院(25.49% vs 34.38%， P <0.05)，暴露组显著低于未暴露组。结论SQABC对高危AMI患者的治疗有明显的益处。
{"title":"Tonifying Qi and activating blood circulation in terms of Traditional Chinese Medicine: their effects in patients with myocardial infarction","authors":"Gao Wulin , Dai Guohua , Zhang Tong , Bi Dongxue , Liu Chunhua , Shi Xiaojing , Zhao Fang , Zhao Chen","doi":"10.1016/S0254-6272(18)30911-7","DOIUrl":"10.1016/S0254-6272(18)30911-7","url":null,"abstract":"<div><h3>OBJECTIVE</h3><p>To investigate the effects of tonifying <em>Qi</em> and activating blood circulation (SQABC), a method in Traditional Chinese Medicine (TCM), on end-point events in patients with myocardial infarction (MI) in this retrospective cohort study.</p></div><div><h3>METHODS</h3><p>Clinical data were obtained from the medical records of patients with acute MI (AMI), both during hospitalization and follow-up, and included general demographic information (age, gender, and contact information), TCM regimens used, and end-point events.</p></div><div><h3>RESULTS</h3><p>A total of 1596 patients with AMI were enrolled to this study, but data of only 1210 cases are accessible till follow-up. We classified the patients based on the exposure levels of SQABC. When comparing the results between all exposure and non-exposure groups, significant differences were identified, both during hospitalization and follow-ups. During hospitalization, cardiac death (4.40% <em>vs</em> 21.55%, <em>P</em> < 0.05) and cardiac shock (3.04% <em>vs</em> 11.62%, <em>P</em> < 0.05) were significantly lower in the exposure group than the non-exposure group. Similarly, during the follow-up, cardiac death (12.04% <em>vs</em> 20.49%, <em>P</em> < 0.05), acute heart failure (7.27% <em>vs</em> 11.81%, <em>P</em> < 0.05), composite endpoint of reinfarction and stroke (9.11% <em>vs</em> 15.28%, <em>P</em> < 0.05), and rehospitalization due to angina (25.49% vs 34.38%, <em>P</em> < 0.05) were significantly lower in the exposure group than the non-exposure group.</p></div><div><h3>CONCLUSION</h3><p>Our findings suggest that SQABC can significantly benefits the subjects in the management of high-risk AMI in them.</p></div>","PeriodicalId":17513,"journal":{"name":"Journal of Traditional Chinese Medicine","volume":null,"pages":null},"PeriodicalIF":2.6,"publicationDate":"2018-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/S0254-6272(18)30911-7","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"74106594","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2018-08-01DOI: 10.1016/S0254-6272(18)30880-X
Jin Yutong , Chen Shan , Xuan Lihua
OBJECTIVE
To evaluate the efficacy and safety of stimulating acupoints in subjects with childhood cough variant asthma (CVA).
METHODS
A Meta-analysis of randomized controlled trials (RCTs) about the comparison between acupoint sticking therapy and non-acupoint sticking therapy for childhood CVA was conducted. The trials’ quality and risk bias were assessed using the Cochrane Handbook tool. Odds ratio (OR) or risk ratio (RR) with 95% confidence intervals (CIs), mean differences (MDs) or standardized mean differences (SMDs) of a random-effects model were calculated. Heterogeneity was assessed by P value and I2 statistics.
RESULTS
Thirteen studies were included in our review, indicating that the total effective rate of stimulating-acupoint group is better than that of control group [RR 1.19, 95% CI (1.13-1.26), P < 0.000 01]. The recurrence rate in two years [RR 0.31, 95% CI (0.19-0.51), P < 0.000 01] and cough duration [MD = −2.42, 95% CI (–3.75, −1.09), P = 0.0004] of childhood CVA in stimulating-acupoint group were significantly lower than those in control group. Besides, stimulating acupoints can reduce the level of IgE [SMD = −0.75, 95% CI (–1.21, −0.30), P = 0.001] and EOS [SMD = −0.36, 95% CI (–0.92,0.21), P =0.22].
CONCLUSION
Our findings suggest that stimulating acupoints had positive effects on childhood CVA and was relatively safe treatment. However, more RCTs with more useful indicators are warrant to confirm the current findings.
目的评价穴位刺激法治疗儿童咳嗽变异性哮喘(CVA)的疗效和安全性。方法对穴位贴敷治疗与非穴位贴敷治疗儿童CVA的随机对照试验进行meta分析。使用Cochrane手册工具评估试验的质量和风险偏倚。计算随机效应模型的95%置信区间(ci)的优势比(OR)或风险比(RR)、平均差异(MDs)或标准化平均差异(SMDs)。采用P值和I2统计量评价异质性。结果纳入13项研究，穴位刺激组总有效率优于对照组[RR 1.19, 95% CI (1.13-1.26)， P <0.000 01]。两年内复发率[RR 0.31, 95% CI (0.19-0.51)， P <刺激穴位组患儿CVA水平与咳嗽持续时间[MD = - 2.42, 95% CI (-3.75， - 1.09)， P = 0.0004]均显著低于对照组。此外，刺激穴位可降低IgE [SMD = - 0.75, 95% CI (-1.21， - 0.30)， P = 0.001]和EOS [SMD = - 0.36, 95% CI (-0.92,0.21)， P =0.22]水平。结论穴位刺激对儿童CVA治疗有积极作用，是一种相对安全的治疗方法。然而，需要更多有更多有用指标的随机对照试验来证实目前的发现。
{"title":"Effect and safety of stimulating acupoints in children with cough variant asthma: A Meta-analysis","authors":"Jin Yutong , Chen Shan , Xuan Lihua","doi":"10.1016/S0254-6272(18)30880-X","DOIUrl":"10.1016/S0254-6272(18)30880-X","url":null,"abstract":"<div><h3>OBJECTIVE</h3><p>To evaluate the efficacy and safety of stimulating acupoints in subjects with childhood cough variant asthma (CVA).</p></div><div><h3>METHODS</h3><p>A Meta-analysis of randomized controlled trials (RCTs) about the comparison between acupoint sticking therapy and non-acupoint sticking therapy for childhood CVA was conducted. The trials’ quality and risk bias were assessed using the Cochrane Handbook tool. Odds ratio (<em>OR</em>) or risk ratio (RR) with 95% confidence intervals (<em>CIs</em>), mean differences (<em>MD</em>s) or standardized mean differences (<em>SMD</em>s) of a random-effects model were calculated. Heterogeneity was assessed by <em>P</em> value and <em>I</em><sup>2</sup> statistics.</p></div><div><h3>RESULTS</h3><p>Thirteen studies were included in our review, indicating that the total effective rate of stimulating-acupoint group is better than that of control group [RR 1.19, 95% <em>CI</em> (1.13-1.26), <em>P</em> < 0.000 01]. The recurrence rate in two years [<em>RR</em> 0.31, 95% <em>CI</em> (0.19-0.51), <em>P</em> < 0.000 01] and cough duration [<em>MD</em> = −2.42, 95% <em>CI</em> (–3.75, −1.09), <em>P</em> = 0.0004] of childhood CVA in stimulating-acupoint group were significantly lower than those in control group. Besides, stimulating acupoints can reduce the level of IgE [SMD = −0.75, 95% <em>CI</em> (–1.21, −0.30), <em>P</em> = 0.001] and EOS [SMD = −0.36, 95% <em>CI</em> (–0.92,0.21), <em>P</em> =0.22].</p></div><div><h3>CONCLUSION</h3><p>Our findings suggest that stimulating acupoints had positive effects on childhood CVA and was relatively safe treatment. However, more RCTs with more useful indicators are warrant to confirm the current findings.</p></div>","PeriodicalId":17513,"journal":{"name":"Journal of Traditional Chinese Medicine","volume":null,"pages":null},"PeriodicalIF":2.6,"publicationDate":"2018-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/S0254-6272(18)30880-X","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"43912091","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2018-08-01DOI: 10.1016/S0254-6272(18)30882-3
Rong Ping , Li Li , Zhang Xilian , Liu Quanhui , Yan Huimin , Xin Deli , Xue Zheng , Ren Xianqing , Wu Zhenqi , Ma Rong , Zhang Junhua
OBJECTIVE
To develop Clinical practice s of Traditional Chinese Medicine (TCM) for acute upper respiratory tract infection (AURI) in children; TCM is used alone or administered together with antibiotics.
METHODS
Under the guidance of evidence-based medicine concept, in strict accordance with the rules of international s development, as well as on the basis of evidence of clinical research of TCM, the s solicited opinions from clinical experts and methodologists in TCM and Western Medicine. GRADE standard was applied to form experts’ consensus.
RESULTS
The s standardized classification of TCM patterns and TCM treatments in children with AURI, including prescription, Chinese patent medicine, non-drug treatment and prevention.
CONCLUSION
Follows the principle of “evidence based, consensus supplemented, and experience referred”, these s were formulated, but the quality of evidence of included studies were relatively low. Further refinement of the s should be needed as deeper clinical studies as available in future.
{"title":"Clinical practices s of Traditional Chinese Medicine for acute upper respiratory tract infection in children","authors":"Rong Ping , Li Li , Zhang Xilian , Liu Quanhui , Yan Huimin , Xin Deli , Xue Zheng , Ren Xianqing , Wu Zhenqi , Ma Rong , Zhang Junhua","doi":"10.1016/S0254-6272(18)30882-3","DOIUrl":"https://doi.org/10.1016/S0254-6272(18)30882-3","url":null,"abstract":"<div><h3>OBJECTIVE</h3><p>To develop Clinical practice s of Traditional Chinese Medicine (TCM) for acute upper respiratory tract infection (AURI) in children; TCM is used alone or administered together with antibiotics.</p></div><div><h3>METHODS</h3><p>Under the guidance of evidence-based medicine concept, in strict accordance with the rules of international s development, as well as on the basis of evidence of clinical research of TCM, the s solicited opinions from clinical experts and methodologists in TCM and Western Medicine. GRADE standard was applied to form experts’ consensus.</p></div><div><h3>RESULTS</h3><p>The s standardized classification of TCM patterns and TCM treatments in children with AURI, including prescription, Chinese patent medicine, non-drug treatment and prevention.</p></div><div><h3>CONCLUSION</h3><p>Follows the principle of “evidence based, consensus supplemented, and experience referred”, these s were formulated, but the quality of evidence of included studies were relatively low. Further refinement of the s should be needed as deeper clinical studies as available in future.</p></div>","PeriodicalId":17513,"journal":{"name":"Journal of Traditional Chinese Medicine","volume":null,"pages":null},"PeriodicalIF":2.6,"publicationDate":"2018-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/S0254-6272(18)30882-3","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"91742994","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2018-08-01DOI: 10.1016/S0254-6272(18)30892-6
Luo Lu , Yu Zheng , Yang Yuyang , Liu Jing , Zhou Wei , Wang Juyi
OBJECTIVE
To explore the advantages of acupuncture treatment guided by channel palpation on stroke-sequel patients.
METHODS
This research was randomized, traditional acupuncture controlled trial using channel palpation acupuncture to treat stroke-sequel patients. Totally 148 patients who were randomly assigned to two experimental groups. The treatment group, i.e, the channel palpation group was treated with Dr. Wang Juyi's Channel Palpation. Patients in control group received acupuncture according to New Century Acupuncture. Every patient was needled at Renzhong (GV 26), Baihui (GV 20), Neiguan (PC 6), Jiquan (HT 1), Chize (LU 5), Weizhong (BL 40), Sanyinjiao (SP 6), Zusanli (ST 36), and each acupuncture treatment was modified according different syndrome differentiations including liver yang rising [Taichong (LR 3), Taixi (KI 3)], wind-phlegm blocking collaterals [Fenglong (ST 40), Hegu (LI 4)], phlegm-heat occupying in the Fu-organs [Quchi (LI 11), Neiting (ST 44), Fenglong (ST 40)], Qi deficiency with blood stasis [Qihai (CV 6), Xuehai (SP 10)], Yin Deficiency with wind [Taixi (KI 3), Fengchi (GB 20)]; for wry mouth, add Jiache (ST 6), Dicang (ST 4); for paralyzed arms, add Jianyu (LI 15), Quchi (LI 11), Shousanli (LI 10) and Hegu (LI 4), for paralyzed legs, add Huantiao (GB 30), Yinlingquan (SP 9) and Fengshi (GB 31). The duration of each treatment was 6 weeks. Then the Fugl-Meyer score, the Stroke Specific Quality of Life scale (SS-QOL), and the National Institute of Health Stroke Scale (NIHSS) were assessed before treatments, after 6 and 12 weeks of treatments to evaluate the acupuncture effect in each group. The data were collected and analyzed after the completion of treatment by SPSS 17 using paired sample t-test.
RESULTS
Totally 148 participants were recruited, and 136 eligible patients were included in this study. The results showed that for FMA motor function and Fugl-Meyer balance function and NIHSS, there is no statistic difference between two groups at the baseline period, after 6-week treatment and after 12-week follow-up (P > 0.05); However, for NIHSS and SS-QOL, there is no statistic difference between two groups at the baseline period and 6 weeks after treatment (P > 0.05). However, statistical difference starts to appear after 12-week (P = 0.028, 0.037 < 0.05).
CONCLUSION
We conclude that as for improving the nerve function and the quality of life, Dr. Wang Juyi's Applied Channel Theory presents a better clinical result.
{"title":"Clinical observation on Wang Juyi's applied channel theory in treating stroke-sequel patients","authors":"Luo Lu , Yu Zheng , Yang Yuyang , Liu Jing , Zhou Wei , Wang Juyi","doi":"10.1016/S0254-6272(18)30892-6","DOIUrl":"10.1016/S0254-6272(18)30892-6","url":null,"abstract":"<div><h3>OBJECTIVE</h3><p>To explore the advantages of acupuncture treatment guided by channel palpation on stroke-sequel patients.</p></div><div><h3>METHODS</h3><p>This research was randomized, traditional acupuncture controlled trial using channel palpation acupuncture to treat stroke-sequel patients. Totally 148 patients who were randomly assigned to two experimental groups. The treatment group, i.e, the channel palpation group was treated with Dr. Wang Juyi's Channel Palpation. Patients in control group received acupuncture according to New Century Acupuncture. Every patient was needled at Renzhong (GV 26), Baihui (GV 20), Neiguan (PC 6), Jiquan (HT 1), Chize (LU 5), Weizhong (BL 40), Sanyinjiao (SP 6), Zusanli (ST 36), and each acupuncture treatment was modified according different syndrome differentiations including liver yang rising [Taichong (LR 3), Taixi (KI 3)], wind-phlegm blocking collaterals [Fenglong (ST 40), Hegu (LI 4)], phlegm-heat occupying in the Fu-organs [Quchi (LI 11), Neiting (ST 44), Fenglong (ST 40)], <em>Qi</em> deficiency with blood stasis [Qihai (CV 6), Xuehai (SP 10)], <em>Yin</em> Deficiency with wind [Taixi (KI 3), Fengchi (GB 20)]; for wry mouth, add Jiache (ST 6), Dicang (ST 4); for paralyzed arms, add Jianyu (LI 15), Quchi (LI 11), Shousanli (LI 10) and Hegu (LI 4), for paralyzed legs, add Huantiao (GB 30), Yinlingquan (SP 9) and Fengshi (GB 31). The duration of each treatment was 6 weeks. Then the Fugl-Meyer score, the Stroke Specific Quality of Life scale (SS-QOL), and the National Institute of Health Stroke Scale (NIHSS) were assessed before treatments, after 6 and 12 weeks of treatments to evaluate the acupuncture effect in each group. The data were collected and analyzed after the completion of treatment by SPSS 17 using paired sample <em>t</em>-test.</p></div><div><h3>RESULTS</h3><p>Totally 148 participants were recruited, and 136 eligible patients were included in this study. The results showed that for FMA motor function and Fugl-Meyer balance function and NIHSS, there is no statistic difference between two groups at the baseline period, after 6-week treatment and after 12-week follow-up (<em>P</em> > 0.05); However, for NIHSS and SS-QOL, there is no statistic difference between two groups at the baseline period and 6 weeks after treatment (<em>P</em> > 0.05). However, statistical difference starts to appear after 12-week (<em>P</em> = 0.028, 0.037 < 0.05).</p></div><div><h3>CONCLUSION</h3><p>We conclude that as for improving the nerve function and the quality of life, Dr. Wang Juyi's Applied Channel Theory presents a better clinical result.</p></div>","PeriodicalId":17513,"journal":{"name":"Journal of Traditional Chinese Medicine","volume":null,"pages":null},"PeriodicalIF":2.6,"publicationDate":"2018-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/S0254-6272(18)30892-6","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"56345453","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
扫码关注我们
求助内容：
应助结果提醒方式：
京公网安备 11010802042870号