Journal of Traditional Chinese Medicine最新文献

{"title":"Effect of spleen-invigorating, Qi-replenishing and blood-arresting formula on zebrafish models with simvastatin-induced hemorrhage caused by spleen failing to control blood, in terms of theory of Traditional Chinese Medicine","authors":"Gao Chong ,&nbsp;Wang Jun ,&nbsp;Wang Jia ,&nbsp;Zhu Xiaoyu ,&nbsp;Zhu Changle ,&nbsp;Guo Shengya ,&nbsp;Wang Chong ,&nbsp;Fan Qiuyue ,&nbsp;Zhang Jianuo ,&nbsp;Xia Bo ,&nbsp;Chen Xinyi","doi":"10.1016/S0254-6272(18)30630-7","DOIUrl":"10.1016/S0254-6272(18)30630-7","url":null,"abstract":"<div><h3>OBJECTIVE</h3><p>To investigate the hemostasis effect of spleen-invigorating, <em>Qi</em>-replenishing and blood-arresting formula, on a zebrafish models with simvastatin-induced hemorrhage, and with symptom pattern caused by spleen failing to control blood, in terms of theory of Traditional Chinese Medicine (TCM).</p></div><div><h3>METHODS</h3><p>In the first experiment, 60 AB strain wild type zebrafishes were randomly assigned into two groups: normal group and model group. The model group was treated with 50 μM simvastatin for 24 h. The second experiment: The melanin allele mutated Albino strain zebrafishes were divided into normal, model, A group and B group. The observational parameters were as follows: blood flow, velocity of movement, hemorrhage ratio and improvement ratio of hemorrhage.</p></div><div><h3>RESULTS</h3><p>Hemorrhage ratio: in the first experiment, brain hemorrhage ratio was 75%. In the second experiment, heart hemorrhage ratio was 65%. Blood flow: compared with the normal group, there was a significantly decrease in the model group (<em>P</em> &lt; 0.001). Velocity of movement: in the first experimental, compared with the normal group, there was a significantly decrease in the model group (<em>P</em> &lt; 0.001). Improvement ratio of hemorrhage: agents A had little effect in heart hemorrhage of the zebrafish; agents B could reduce heart hemorrhage ratio of the zebrafish, and increase the improvement ratio of hemorrhage.</p></div><div><h3>CONCLUSION</h3><p>The manifestation of zebrafish model with simvastatin-induced hemorrhage is basically similar to that of the clinical symptom pattern caused by spleen's failure to control blood. The Spleen-invigorating, <em>Qi</em>-replenishing and Blood-arresting Formula can reduce the heart hemorrhage ratio of zebrafish induced by simvastatin, and increase the Improvement ratio of hemorrhage.</p></div>","PeriodicalId":17513,"journal":{"name":"Journal of Traditional Chinese Medicine","volume":"38 3","pages":"Pages 399-405"},"PeriodicalIF":2.6,"publicationDate":"2018-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/S0254-6272(18)30630-7","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"43266101","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Jinghua Weikang capsule protects against Helicobacter pylori-induced inflammatory responses via the nuclear factor-kappa B signaling pathway","authors":"Shi Zongming ,&nbsp;Ye Hui ,&nbsp;Yu Jing ,&nbsp;Cheng Hong ,&nbsp;Li Jiang ,&nbsp;Zhang Xuezhi","doi":"10.1016/j.jtcm.2018.03.001","DOIUrl":"10.1016/j.jtcm.2018.03.001","url":null,"abstract":"<div><h3>OBJECTIVE</h3><p>To investigate the inhibitory effect of Jinghua Weikang capsule (JWC) on gastric inflammation induced by Helicobacter pylori (H. pylori) <em>via</em> the nuclear factor-kappa B (NF-κB) signaling pathway in Kunming mice.</p></div><div><h3>METHODS</h3><p>We investigated the anti-inflammation potential of JWC extract <em>in vivo</em> in a H. pylori-induced gastritis mouse model. The expression of inflammation-related molecules was evaluated by Western blotting, and the concentrations of <em>in vivo</em> inflammatory markers were measured by enzyme-linked immunosorbent assay. Inflammatory cell infiltration was evaluated by histopathological examination, and mRNA levels of related genes were evaluated by quantitative reverse transcription polymerase chain reaction.</p></div><div><h3>RESULTS</h3><p>JWC had a dose-dependent protective effect against H. pylori-induced gastritis by protecting gastric epithelial cells and inhibiting inflammatory cell infiltration. Mechanistically, JWC decreased the protein levels of phosphorylated IκBα and NF-κB p65, mRNA levels of NF-κB pathway molecules, and plasma levels of tumor necrosis factor-α and interleukin 1 beta.</p></div><div><h3>CONCLUSION</h3><p>An important finding of our study is that JWC attenuated gastrointestinal inflammation and ulceration and exerted a protective effect against gastric injury <em>via</em> inhibition of inflammation reactions and regulating the canonical NF-κB signaling pathway <em>in vivo.</em></p></div>","PeriodicalId":17513,"journal":{"name":"Journal of Traditional Chinese Medicine","volume":"38 3","pages":"Pages 366-372"},"PeriodicalIF":2.6,"publicationDate":"2018-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/j.jtcm.2018.03.001","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"46953765","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Twirling reinforcing-reducing manipulation — central mechanism underlying antihypertensive effect on spontaneous hypertension in rats","authors":"Guo Qiulei ,&nbsp;Liu Qingguo ,&nbsp;Sun Dongmei ,&nbsp;Nie Binbin","doi":"10.1016/S0254-6272(18)30629-0","DOIUrl":"10.1016/S0254-6272(18)30629-0","url":null,"abstract":"<div><h3>OBJECTIVE</h3><p>To investigate antihypertensive effect in rats in order to confirm that twirling reinforcing-reducing manipulation (TRRM) might be the central mechanism underlying the action.</p></div><div><h3>METHODS</h3><p>In the study, <span>¹⁸</span>F-2-fluoro-deoxy-D-glucose positron emission tomography (<span>¹⁸</span>F-FDG-PET) was employed. Fifity-six spontaneous hypertensive rats (SHRs) were randomly divided into a model group, a single-needle acupuncture (SNA) group, a twirling reinforcing group (SNA + TRF) and a twirling reducing (SNA + TRD) group. Fourteen Wistar rats were assigned to the control group. The acupuncture intervention at Taichong (LR 3) acupoint was administered once daily in the SNA, SNA + TRF and SNA + TRD groups for 14 days, with 1 d interval between the two weeks. The blood pressure (BP) of all rats was measured repeatedly and <span>¹⁸</span>F-FDG-PET scans were conducted on the 14th day. PET images were processed with Statistical Parametric Mapping 8.0.</p></div><div><h3>RESULTS</h3><p>After the intervention, systolic BP showed a significant decrease in the SNA, SNA+TRF and SNA + TRD versus the model groups (all <em>P</em> &lt; 0.01) and in the SNA + TRF and SNA + TRD versus the SNA groups (both <em>P</em> &lt; 0.01), with the SNA + TRD group exhibited the best antihypertensive effect (<em>P</em> &lt; 0.01). The key brain regions activated by TRRM were mainly concentrated in the cerebellum, hippocampus, hypothalamus, medulla oblongata, insular cortex, midbrain, thalamus and visual cortex.</p></div><div><h3>CONCLUTION</h3><p>TRRM could significantly lower the BP of SHRs by improving the cerebral glucose metabolism of the activated key brain regions and the underlying central mechanism may be related to the central rennin-angiotensin system and neurotransmission.</p></div>","PeriodicalId":17513,"journal":{"name":"Journal of Traditional Chinese Medicine","volume":"38 3","pages":"Pages 391-398"},"PeriodicalIF":2.6,"publicationDate":"2018-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/S0254-6272(18)30629-0","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"43465907","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effect of optimal combination of Huangqi (Radix Astragali Mongolici) and Ezhu (Rhizoma Curcumae Phaeocaulis) on proliferation and apoptosis of A549 lung cancer cells","authors":"Xu Chengyong ,&nbsp;Wang Yuguo ,&nbsp;Feng Jian ,&nbsp;Qin Li ,&nbsp;Xu Ran ,&nbsp;Dou Yongqi","doi":"10.1016/S0254-6272(18)30625-3","DOIUrl":"10.1016/S0254-6272(18)30625-3","url":null,"abstract":"<div><h3>OBJECTIVE</h3><p>To investigate the effect of optimal combination (E) of Huangqi (<em>Radix Astragali Mongolici</em>) and Ezhu (<em>Rhizoma Curcumae Phaeocaulis</em>) on proliferation and apoptosis of A549 lung cancer cells and the possible mechanism underpinning the action.</p></div><div><h3>METHODS</h3><p>A uniform design method was used to optimize the E of Huangqi (<em>Radix Astragali Mongolici</em>) and Ezhu (<em>Rhizoma Curcumae Phaeocaulis</em>) in A549 lung cancer cells. MTS assay was applied to analyze the effect of the component formula of Huangqi (<em>Radix Astragali Mongolici</em>) and Ezhu (<em>Rhizoma Curcumae Phaeocaulis</em>) on A549 cells viability in various uniform design groups. A549 cells with exponential growth in routine culture were exposed to CoCl₂ (200 μmol/L) to mimic hypoxic conditions. Group 0 was treated with RPMI-1640, the group CoCl₂ was treated with CoCl₂ (200 μmol/L), the group DDP + CoCl₂ was treated with 4 mg/L Cisplatin injection (DDP) + CoCl₂ (200 μmol/L), and the drug group was treated with various dose of E (0.5E, 1E, 2E) + CoCl₂ (200 μmol/L). All groups were cultured for 24 h. Cell apoptosis was measured by Annexin V-FITC/propidium iodide double staining and flow cytometry. Western blot assay and quantitative real-time polymerase chain reaction (qRT-PCR) were employed to detect the protein and mRNA expression of B-celllymphoma-2 (Bcl-2), Bcl-2-associated X protein (Bax) and cysteinyl aspartate specific proteinase-3 (caspase-3).</p></div><div><h3>RESULTS</h3><p>The E obtained by the uniform design was comprise of 200 mg/L Astragalus polysaccharide (X1) and 32 mg/L Curcumin (X3). Group DDP+ CoCl2, group 1E + CoCl₂ and group 2E + CoCl2 promoted the apoptosis of A549 cells (<em>P</em> &lt; 0.05). Group 1E + CoCl₂ and group 2E + CoCl₂ had no statistically significant differences compared with the group DDP + CoCl₂ (<em>P</em> &gt; 0.05). Compared with group 0, various doses of E + CoCl₂ could up-regulate the expression of Bax and caspase-3 and down-regulate the expression of Bcl-2 at protein and mRNA levels (<em>P</em> &lt; 0.05).</p></div><div><h3>CONCLUSION</h3><p>Astragalus polysaccharide and Curcumin was the optimal combination of Huangqi (<em>Radix Astragali Mongolici</em>) and Ezhu (<em>Rhizoma Curcumae Phaeocaulis</em>). E promoted the apoptosis of A549 cells. Combination of Astragalus polysaccharide and Curcumin increased the expression of Bax and caspase-3, and decreased the expression of Bcl-2 to initiate apoptosis in A549 cells under chemical-induced hypoxia.</p></div>","PeriodicalId":17513,"journal":{"name":"Journal of Traditional Chinese Medicine","volume":"38 3","pages":"Pages 351-358"},"PeriodicalIF":2.6,"publicationDate":"2018-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/S0254-6272(18)30625-3","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"43596924","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Persian medicine non-pharmacological therapies for headache: phlebotomy and wet cupping","authors":"Akramo Sadat Atyabi ,&nbsp;Fatemeh Nejatbakhsh ,&nbsp;Hoorie Mohammadi Kenari ,&nbsp;Fatemeh Eghbalian ,&nbsp;Mohammad Hossein Ayati ,&nbsp;Laila Shirbeigi","doi":"10.1016/S0254-6272(18)30639-3","DOIUrl":"10.1016/S0254-6272(18)30639-3","url":null,"abstract":"<div><h3>OBJECTIVE</h3><p>To describe the common causes of headache as well as the different methods of Phlebotomy (Fasd) and wet Cupping (Hijamat) based on Persian Medicine.</p></div><div><h3>METHODS</h3><p>In this study, we searched Google Scholar, Science Direct, PubMed and Scopus to get any in vitro or clinical evidence of the efficacy and possible mechanisms of phlebotomy or wet cupping on different types of headaches. Also different types of headaches, according to PM, under the title of “Sodaa”; extracted from famous Persian medical text books with key words including “Sodaa”, “Bayze” and “Shaqiqe”.</p></div><div><h3>RESULTS</h3><p>According to PM, diseases (dystemperament) are created by abnormal alteration in temperament. Temperament is a monolithic quality yielded by interaction between opposing qualities of four elements that are coldness, hotness, dryness and wetness. Headaches are caused by dystemperament (sue-Mizaj) in brain or in total body that are divided into simple and corporal. Common forms of the latter type are Vaporal and Migrant headache (Bokhari and Rihi). Each of these headaches is treated either through phlebotomy or wet cupping.</p></div><div><h3>CONCLUSION</h3><p>In Persian Medicine, Fasd and Hijamat are easy and economical methods for treatment of headache with reasonable results. By describing the precise areas and indications of phlebotomy and wet cupping, this study provides the first step for future clinical trials in this field.</p></div>","PeriodicalId":17513,"journal":{"name":"Journal of Traditional Chinese Medicine","volume":"38 3","pages":"Pages 457-464"},"PeriodicalIF":2.6,"publicationDate":"2018-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/S0254-6272(18)30639-3","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"47172701","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Application of Traditional Chinese Medicine Four-diagnostic auxiliary apparatus in evaluation of health status and clinical treatment","authors":"Chen Tianyu ,&nbsp;Niu Tingli ,&nbsp;Niu Xin ,&nbsp;Si Yingchu ,&nbsp;Yang Xuezhi ,&nbsp;Ma Liangxiao","doi":"10.1016/S0254-6272(18)30637-X","DOIUrl":"10.1016/S0254-6272(18)30637-X","url":null,"abstract":"<div><h3>OBJECTIVE</h3><p>To evaluate the application of Traditional Chinese Medicine (TCM) Four-diagnostic Auxiliary Apparatus in disease diagnosis.</p></div><div><h3>METHODS</h3><p>The liver cancer patients and healthy controls were recruited from Shanghai Integrated Chinese and Western Medicine Hospital and Beijing University of Traditional Chinese Medicine, respectively. Then, the included subjects were diagnosed by the Four-diagnostic auxiliary apparatus.</p></div><div><h3>RESULTS</h3><p>Thirty liver cancer patients and 30 paired healthy controls were enrolled in this study. Based on the apparatus, the pulse wave velocity was significantly higher in patients compared with controls (<em>P</em> &lt; 0.05). The number of patients with purple tongue and ecchymosis were more than controls (<em>P</em> &lt; 0.05). The number of patients (10%) with yellow tongue coating were higher than the controls (0%). Patients were inclined to be with water type and fire type constitution.</p></div><div><h3>CONCLUSION</h3><p>TCM Four-diagnostic auxiliary apparatus can be applied in clinical diagnosis of body constitution and health status of subjects. It promotes the accuracy and speed for disease diagnosis and TCM standardization.</p></div>","PeriodicalId":17513,"journal":{"name":"Journal of Traditional Chinese Medicine","volume":"38 3","pages":"Pages 447-451"},"PeriodicalIF":2.6,"publicationDate":"2018-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/S0254-6272(18)30637-X","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"76064896","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effect of Sini decoction on angiotensin II, transforming growth factor β1 and connective tissue growth factor in rats with myocardial fibrosis-induced banding of the abdominal aorta","authors":"Shi Yueping ,&nbsp;Zhai Jianli ,&nbsp;Liu Chunxiao","doi":"10.1016/S0254-6272(18)30634-4","DOIUrl":"10.1016/S0254-6272(18)30634-4","url":null,"abstract":"<div><h3>OBJECTIVE</h3><p>To investigate the effect of Sini decoction on rats with myocardial fibrosis induced by banding the abdominal aorta, and explore the mechanism underlying its actions on angiotensin II (Ang II), transforming growth factor-β₁ (TGF-β₁) and connective tissue growth factor (CTGF).</p></div><div><h3>METHODS</h3><p>Forty-eight male Sprague-Dawley rats were randomly divided into sham operation, model, Captopril, and Sini decoction groups. The models were established by the partial banding of the abdominal aorta according to Doering's method. Eight weeks later, heart weight indexes were calculated; hemodynamic changes of the hearts were tested; changes in myocardial tissue morphology were observed by Masson staining; and myocardial collagen volume fraction was calculated. Enzyme-linked immunosorbent assay was used to measure the concentration of Ang II in serum. The expression of TGF-β₁ and CTGF were determined by immunohistochemistry and Western blotting.</p></div><div><h3>RESULTS</h3><p>Compared with the sham operation group, the heart weight index, collagen volume fraction of the myocardium, serum levels of Ang II, and the expression of myocardial TGF-β₁ and CTGF in the model group were significantly increased (<em>P</em> &lt; 0.05). Compared with the model group, the heart weight index, collagen volume fraction of the myocardium, serum levels of Ang II, and the expression of myocardial TGF-β₁ and CTGF in all treatment groups were significantly reduced (<em>P</em> &lt; 0.05).</p></div><div><h3>CONCLUSION</h3><p>Sini decoction reduced Ang II level and inhibited the expression of myocardial TGF-β₁ and CTGF, which may explain the mechanism of its protective effect on myocardium with fibrosis.</p></div>","PeriodicalId":17513,"journal":{"name":"Journal of Traditional Chinese Medicine","volume":"38 3","pages":"Pages 427-432"},"PeriodicalIF":2.6,"publicationDate":"2018-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/S0254-6272(18)30634-4","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"43798310","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"In comparison with vitamin C and butylated hydroxytoluene, the antioxidant capacity of aqueous extracts from buds and flowers of Lonicera japonica Thunb.","authors":"Zhou Yunfeng ,&nbsp;Li Lin ,&nbsp;Sun Lan ,&nbsp;Zhou Lidong ,&nbsp;Xu Yang","doi":"10.1016/S0254-6272(18)30627-7","DOIUrl":"10.1016/S0254-6272(18)30627-7","url":null,"abstract":"<div><h3>OBJECTIVE</h3><p>To assess the antioxidant capacity of aqueous extracts of buds and flowers of Lonicera japonica Thunb. (BLJ and FLJ) using <em>in vitro</em> assays.</p></div><div><h3>METHODS</h3><p>We assessed the <em>in vitro</em> antioxidant activities of aqueous extracts of BLJ and FLJ and compared with that of classical antioxidants vitamin C and butylated hydroxytoluene, using several well-established methods including the 1,1-diphenyl-2-picryl-hydrazyl assay, 2,2'-azino-bis (3-ethylbenzthiazoline-6-sulfonic acid) assay, reducing power assay, fluorescence recovery after photo-bleaching assay, β-carotene bleaching assay, ferric thiocyanate assay, and thiobarbituric acid method.</p></div><div><h3>RESULTS</h3><p>The aqueous extracts of both BLJ and FLJ had similarly potent antioxidant capacity. There were no significance differences between BLJ and FLJ in all the assays.</p></div><div><h3>CONCLUSION</h3><p>The aqueous extracts of both BLJ and FLJ have antioxidant activity with comparable efficacy. These findings suggest that both BLJ and FLJ may have the potential as natural antioxidants.</p></div>","PeriodicalId":17513,"journal":{"name":"Journal of Traditional Chinese Medicine","volume":"38 3","pages":"Pages 373-379"},"PeriodicalIF":2.6,"publicationDate":"2018-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/S0254-6272(18)30627-7","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"46602436","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Protective effects of Jiayan Kangtai granules on autoimmune thyroiditis in a rat model by modulating Th17/Treg cell balance","authors":"Hou Yi ,&nbsp;Wang Tieshan ,&nbsp;Guo Xiangyu ,&nbsp;Sun Wen ,&nbsp;Guo Xuan ,&nbsp;Wu Lili ,&nbsp;Qin Lingling ,&nbsp;Zhang Chengfei ,&nbsp;Liu Tonghua","doi":"10.1016/S0254-6272(18)30628-9","DOIUrl":"10.1016/S0254-6272(18)30628-9","url":null,"abstract":"<div><h3>OBJECTIVE</h3><p>To investigate the protective effects of Jiayan Kangtai (JYKT) granules, consisting of 9 Chinese herbs, in a rat model of autoimmune thyroiditis (AIT), and the possible underlying mechanism.</p></div><div><h3>METHODS</h3><p>Female Lewis rats (6–8 weeks) were randomly apportioned to 5 groups of 10, including a normal control. AIT was induced in the untreated AIT-model group, and rats treated subsequently with daily low, medium, or high dose JYKT granules. After 12 weeks, plasma levels of thyroid autoantibodies and morphological changes in the thyroid were detected by enzyme-linked immunosorbent assay and histological examination, respectively. The presence of interleukin (IL)-6, IL23p19, and IL-2 in thyroid tissue was assessed by immunohistochemical staining. The percentages of T helper (Th)17 cells and regulatory T cells (Tregs) in the peripheral blood were analyzed by flow cytometry. Relevant levels of cytokines and proteins were examined <em>via</em> bead-based multiplex flow cytometry and ELISA, respectively. Expressions of genes and proteins regulated by Th17 cells and Tregs were shown by real-time PCR and Western blot.</p></div><div><h3>RESULTS</h3><p>Compared to the control, AIT-model rats had higher plasma concentrations of thyroid autoantibodies. The high-dose JYKT rats showed significantly lower levels of thyroid autoantibodies compared with the AIT model group. Rats in the AIT-JYKT groups also had fewer thyroid lesions and less lymphocytic infiltration, a lower percentage of Th17 cells, and a higher percentage of Tregs, compared with the AIT-model. Rats given high-dose JYKT had a significantly lower Th17/Treg ratio compared with the AIT model. Differences in plasma cytokine concentrations and relevant gene and protein expressions in the spleens of JYKT-treated rats and the AIT group suggested an association between JYKT treatment and lower Th17 cell percentage and higher Treg activity.</p></div><div><h3>CONCLUSION</h3><p>JYKT treatment appeared to be protective against AIT in rats, possibly <em>via</em> the regulation of the Th17 cell/Treg imbalance in AIT.</p></div>","PeriodicalId":17513,"journal":{"name":"Journal of Traditional Chinese Medicine","volume":"38 3","pages":"Pages 380-390"},"PeriodicalIF":2.6,"publicationDate":"2018-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/S0254-6272(18)30628-9","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41717740","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Observation of changes in the number of myocardial capillaries in rabbits after treatment of acute myocardial infarction by Tongxinluo superfine powder","authors":"Wu Haotian ,&nbsp;Zhang Fengshun ,&nbsp;Liu Jinyi ,&nbsp;Li Xuezhao ,&nbsp;Guo Fei ,&nbsp;Wu Yiling","doi":"10.1016/S0254-6272(18)30631-9","DOIUrl":"10.1016/S0254-6272(18)30631-9","url":null,"abstract":"<div><h3>OBJECTIVE</h3><p>To investigate the effects of Tongxinluo superfine powder on cardiac function, infarct size and the number of myocardial capillaries in a rabbit model of acute myocardial infarction.</p></div><div><h3>METHODS</h3><p>A total of 32 New Zealand white rabbits were randomly divided into four groups: sham operation group, model group, treatment group, and pre-treatment, the experiment of pre-treatment group was performed 6 weeks early than the treat) group, The four groups use a unified modeling technique. An acute myocardial infarction model was established through external application of 70% ferric chloride on the coronary artery. After 7 d, electrocardiogram, ultrasonography of cardiac function, micro-computed tomography, pathology and other data were collected.</p></div><div><h3>RESULTS</h3><p>In the treatment and pre-treatment groups, ejection fraction, left ventricular short axis shortening rate, left ventricular end-systolic diameter and cardiac output significantly improved, the number of capillaries significantly increased, and infarct size significantly decreased. In addition, the results suggest that the value of intra-ventricular pressure and the situation of electrocardiogram also changed to different degrees with the increasing of treatment of cycle.</p></div><div><h3>CONCLUSION</h3><p>Tongxinluo superfine powder can protect the myocardium, improve the blood supply of the myocardium and reduce the degree of myocardial injury, during acute stage of myocardial infarction.</p></div>","PeriodicalId":17513,"journal":{"name":"Journal of Traditional Chinese Medicine","volume":"38 3","pages":"Pages 406-411"},"PeriodicalIF":2.6,"publicationDate":"2018-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/S0254-6272(18)30631-9","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"47199159","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}