Journal of Traditional Chinese Medicine最新文献_第3页

Exploring pathogenesis in subjects with subjective Tinnitus having kidney deficiency pattern in terms of Traditional Chinese Medicine based on serum metabolic profiles 基于血清代谢谱探讨肾虚型主观性耳鸣的中医发病机制

IF 2.6 4区医学 Q2 INTEGRATIVE & COMPLEMENTARY MEDICINE

Journal of Traditional Chinese Medicine

Pub Date : 2018-10-01 DOI: 10.1016/S0254-6272(18)30918-X

Tan Yong , Shen Shanshan , Guan Fulan , He Bing , Lu Cheng , Xiao Cheng , Jiang Miao , Zhao Ning , Li Li , Cheng Shiping , Zu Xianpeng , Zhang Weidong , Liu Xinru , Lü Aiping

OBJECTIVE

To investigate the metabolic pathogenesis in subjects with subjective tinnitus (ST) having kidney deficiency pattern (KDP) (ST/KDP) in terms of Traditional Chinese Medicine.

METHODS

Three groups of subjects, including healthy individuals, subjects with ST/KDP, and subjects who were healthy initially and then developed ST/KDP one year later (healthy → ST/KDP), were recruited for this study. Serum metabolic profiles of all subjects were analyzed using ultra-performance liquid chromatography coupled with quadruple-time-of-flight mass spectrometry. The metabolic characteristics of the ST/KDP subjects were determined, and the corresponding biomarkers were predicted. The metabolomics data from the healthy → ST/KDP subjects were collected for further verification.

RESULTS

Twelve metabolites in the ST/KDP subjects were different from those of the healthy control subjects. Of these metabolites, according to the prediction, except for octanoic acid, other metabolites might characterize ST/KDP. Ten metabolites at the outcome ST/KDP stage were different from those at the initial (control) stage. Through the comparison of these metabolites with the predicted metabolites, five common metabolites, including upregulated glutamate, serotonin, orotic acid and 8-oxoguanine, as well as downregulated taurine, were found. These common metabolites were significantly associated with canonical pathways including calcium signaling, γ-aminobutyric acid (GABA) receptor signaling, purine and pyrimidine biosynthesis, taurine biosynthesis, and serotonin receptor signaling.

CONCLUSION

The metabolic pathogenesis in ST/KDP subjects was characterized by upregulated glutamate, serotonin, orotic acid and 8-oxoguanine, as well as downregulated taurine, additionally, perturbations of calcium signaling, GABA receptor signaling, purine and pyrimidine biosynthesis, taurine biosynthesis, and serotonin receptor signaling.

目的从中医角度探讨肾虚型(ST/KDP)主观性耳鸣(ST/KDP)患者的代谢发病机制。方法将健康个体、ST/KDP患者和ST/KDP患者(健康→ST/KDP)分为3组进行研究。采用超高效液相色谱联用四倍飞行时间质谱法分析所有受试者的血清代谢谱。测定ST/KDP受试者的代谢特征，并预测相应的生物标志物。收集健康→ST/KDP受试者的代谢组学数据进行进一步验证。结果ST/KDP组12种代谢物与健康对照组存在差异。在这些代谢物中，根据预测，除辛酸外，其他代谢物可能是ST/KDP的特征。结果ST/KDP阶段的10种代谢物与初始(对照)阶段不同。将这些代谢物与预测代谢物进行比较，发现谷氨酸、血清素、山茱萸酸和8-氧鸟嘌呤上调、牛磺酸下调等5种常见代谢物。这些常见代谢物与钙信号、γ-氨基丁酸(GABA)受体信号、嘌呤和嘧啶生物合成、牛磺酸生物合成和5 -羟色胺受体信号等典型信号通路显著相关。结论ST/KDP患者的代谢发病机制以谷氨酸、5 -羟色胺、山羊酸和8-氧鸟嘌呤上调，牛磺酸下调，钙信号、GABA受体信号、嘌呤和嘧啶生物合成、牛磺酸生物合成和5 -羟色胺受体信号干扰为特征。

{"title":"Exploring pathogenesis in subjects with subjective Tinnitus having kidney deficiency pattern in terms of Traditional Chinese Medicine based on serum metabolic profiles","authors":"Tan Yong , Shen Shanshan , Guan Fulan , He Bing , Lu Cheng , Xiao Cheng , Jiang Miao , Zhao Ning , Li Li , Cheng Shiping , Zu Xianpeng , Zhang Weidong , Liu Xinru , Lü Aiping","doi":"10.1016/S0254-6272(18)30918-X","DOIUrl":"10.1016/S0254-6272(18)30918-X","url":null,"abstract":"<div><h3>OBJECTIVE</h3><p>To investigate the metabolic pathogenesis in subjects with subjective tinnitus (ST) having kidney deficiency pattern (KDP) (ST/KDP) in terms of Traditional Chinese Medicine.</p></div><div><h3>METHODS</h3><p>Three groups of subjects, including healthy individuals, subjects with ST/KDP, and subjects who were healthy initially and then developed ST/KDP one year later (healthy → ST/KDP), were recruited for this study. Serum metabolic profiles of all subjects were analyzed using ultra-performance liquid chromatography coupled with quadruple-time-of-flight mass spectrometry. The metabolic characteristics of the ST/KDP subjects were determined, and the corresponding biomarkers were predicted. The metabolomics data from the healthy → ST/KDP subjects were collected for further verification.</p></div><div><h3>RESULTS</h3><p>Twelve metabolites in the ST/KDP subjects were different from those of the healthy control subjects. Of these metabolites, according to the prediction, except for octanoic acid, other metabolites might characterize ST/KDP. Ten metabolites at the outcome ST/KDP stage were different from those at the initial (control) stage. Through the comparison of these metabolites with the predicted metabolites, five common metabolites, including upregulated glutamate, serotonin, orotic acid and 8-oxoguanine, as well as downregulated taurine, were found. These common metabolites were significantly associated with canonical pathways including calcium signaling, γ-aminobutyric acid (GABA) receptor signaling, purine and pyrimidine biosynthesis, taurine biosynthesis, and serotonin receptor signaling.</p></div><div><h3>CONCLUSION</h3><p>The metabolic pathogenesis in ST/KDP subjects was characterized by upregulated glutamate, serotonin, orotic acid and 8-oxoguanine, as well as downregulated taurine, additionally, perturbations of calcium signaling, GABA receptor signaling, purine and pyrimidine biosynthesis, taurine biosynthesis, and serotonin receptor signaling.</p></div>","PeriodicalId":17513,"journal":{"name":"Journal of Traditional Chinese Medicine","volume":"38 5","pages":"Pages 773-780"},"PeriodicalIF":2.6,"publicationDate":"2018-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/S0254-6272(18)30918-X","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"85539195","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Weipixiao ameliorates gastric precancerous lesions in a rat's model by regulating GSK3β and C-myc 胃皮消通过调节GSK3β和C-myc改善大鼠胃癌前病变模型

IF 2.6 4区医学 Q2 INTEGRATIVE & COMPLEMENTARY MEDICINE

Journal of Traditional Chinese Medicine

Pub Date : 2018-10-01 DOI: 10.1016/S0254-6272(18)30909-9

Zeng Jinhao , Pan Huafeng , Guo Jing , Gong Daoyin , Cai Tiantian , Chen Xiaodong , Zhang Yi , You Fengming , Chen Longhui , Zhao Ziming , Liang Chao

OBJECTIVE

To investigate the mechanism underlying the action of Weipixiao (WPX) in a rat's model with ameliorating gastric precancerous lesions (GPL).

METHODS

HPLC analysis was performed to identify the chemical constituents of WPX preparation. Sprague- Dawley rats were randomly assigned into control group, model group, vitacoenzyme group, high-dose WPX group (H-WPX), medium-dose WPX group (M-WPX) and low-dose WPX group (L-WPX). After modeling, the treated rats were administrated WPX or vitacoenzyme intragastrically for consecutive 10 weeks. Gene and protein expressions of GSK3β, C-myc, Cylin E were evaluated by quantitative real-time reverse transcription-polymerase chain reaction (RT-qPCR) and immunohistochemistry, respectively.

RESULTS

WPX could efficiently attenuate the pathological alterations of “non-progressive GPL” in rats. As expected, mRNA and protein levels of C-myc and Cylin E were up-regulated in model rats, while GSK3β expression down-regulated (P < 0.01). WPX treatment, especially at low dose, could significantly down-regulate the mRNA as well as protein levels of C-myc, and could lead to remarkable up-regulation of mRNA and protein levels of GSK3β in GPL rats (P < 0.05). However, no significant changes were observed in WPX-treated rats.

CONCLUSION

Our findings suggested that WPX-mediated attenuation of GPL pathological alterations might be due to its regulatory effect on the expressions of GSK3β and C-myc, and on the dysregulation of Wnt/GSK3β pathway.

目的探讨胃脾消(WPX)对大鼠胃癌前病变(GPL)的改善作用机制。方法采用高效液相色谱法对WPX制剂进行化学成分鉴定。将Sprague- Dawley大鼠随机分为对照组、模型组、维生素酶组、高剂量WPX组(H-WPX)、中剂量WPX组(M-WPX)和低剂量WPX组(L-WPX)。造模后，给药大鼠灌胃WPX或维酵素，连续10周。采用实时定量逆转录聚合酶链反应(RT-qPCR)和免疫组织化学分别检测GSK3β、C-myc、Cylin E基因和蛋白的表达。结果swpx能有效减轻大鼠“非进行性GPL”的病理改变。正如预期的那样，模型大鼠C-myc和Cylin E的mRNA和蛋白水平上调，而GSK3β的表达下调(P <0.01)。WPX处理，特别是低剂量处理，可以显著下调GPL大鼠C-myc mRNA和蛋白水平，并导致GSK3β mRNA和蛋白水平显著上调(P <0.05)。然而，在wpx治疗的大鼠中没有观察到明显的变化。结论wpx介导的GPL病理改变的减弱可能与其对GSK3β和C-myc的表达以及对Wnt/GSK3β通路失调的调节作用有关。

{"title":"Weipixiao ameliorates gastric precancerous lesions in a rat's model by regulating GSK3β and C-myc","authors":"Zeng Jinhao , Pan Huafeng , Guo Jing , Gong Daoyin , Cai Tiantian , Chen Xiaodong , Zhang Yi , You Fengming , Chen Longhui , Zhao Ziming , Liang Chao","doi":"10.1016/S0254-6272(18)30909-9","DOIUrl":"10.1016/S0254-6272(18)30909-9","url":null,"abstract":"<div><h3>OBJECTIVE</h3><p>To investigate the mechanism underlying the action of Weipixiao (WPX) in a rat's model with ameliorating gastric precancerous lesions (GPL).</p></div><div><h3>METHODS</h3><p>HPLC analysis was performed to identify the chemical constituents of WPX preparation. Sprague- Dawley rats were randomly assigned into control group, model group, vitacoenzyme group, high-dose WPX group (H-WPX), medium-dose WPX group (M-WPX) and low-dose WPX group (L-WPX). After modeling, the treated rats were administrated WPX or vitacoenzyme intragastrically for consecutive 10 weeks. Gene and protein expressions of GSK3β, C-myc, Cylin E were evaluated by quantitative real-time reverse transcription-polymerase chain reaction (RT-qPCR) and immunohistochemistry, respectively.</p></div><div><h3>RESULTS</h3><p>WPX could efficiently attenuate the pathological alterations of “non-progressive GPL” in rats. As expected, mRNA and protein levels of C-myc and Cylin E were up-regulated in model rats, while GSK3β expression down-regulated (<em>P</em> < 0.01). WPX treatment, especially at low dose, could significantly down-regulate the mRNA as well as protein levels of C-myc, and could lead to remarkable up-regulation of mRNA and protein levels of GSK3β in GPL rats (<em>P</em> < 0.05). However, no significant changes were observed in WPX-treated rats.</p></div><div><h3>CONCLUSION</h3><p>Our findings suggested that WPX-mediated attenuation of GPL pathological alterations might be due to its regulatory effect on the expressions of GSK3β and C-myc, and on the dysregulation of Wnt/GSK3β pathway.</p></div>","PeriodicalId":17513,"journal":{"name":"Journal of Traditional Chinese Medicine","volume":"38 5","pages":"Pages 705-713"},"PeriodicalIF":2.6,"publicationDate":"2018-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/S0254-6272(18)30909-9","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41318371","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 3

Effect of the herbal medicine Danggui Sini plus Wuzhuyu Shengjiang Tang on erythrocyte deformability in normal subjects: a cross-over trial 当归四逆加五柱瘀生姜汤对正常人红细胞变形能力的影响:交叉试验

IF 2.6 4区医学 Q2 INTEGRATIVE & COMPLEMENTARY MEDICINE

Journal of Traditional Chinese Medicine

Pub Date : 2018-10-01 DOI: 10.1016/S0254-6272(18)30917-8

JiEun Lee , Seung-Yeon Cho , Seong-Uk Park , Woo-Sang Jung , Sang-Kwan Moon , Jung-Mi Park , Chang-Nam Ko , Ki-Ho Cho , Seungwon Kwon

OBJECTIVE

To show whether Danggui Sini plus Wuzhuyu Shengjiang Tang (DSWST) has any transient effect on erythrocyte deformability in normal subjects.

METHODS

A total of 25 subjects [mean age (27.8 ± 1.8) years] was enrolled in this study. The study was designed as a cross-over trial in which the subjects took part for 2 d. On the first day, blood samples were collected at baseline and 1-2 h after administration of water, whereas, on the second day, instead of water, the subjects were administered DSWST after the baseline blood sampling. The blood samples collected at baseline and after the administration water or DSWST, were examined for erythrocyte deformability.

RESULTS

The elongation index increased significantly after 2 h (P = 0.009) compared to the baseline after DSWST intake. However, after water intake, there was no significant difference observed. When comparing the percent change of erythrocyte deformability between DSWST and water, we found that after 2 h of administration, DSWST improved erythrocyte deformability significantly compared to water (P < 0.001).

CONCLUSION

DSWST has a transient effect on erythrocyte deformability in normal subjects.

目的观察当归四逆联合五柱瘀生姜汤对正常人红细胞变形能力的影响。方法共纳入25例受试者，平均年龄(27.8±1.8)岁。该研究被设计为交叉试验，受试者参加2天。第一天，在基线和给水后1-2小时采集血液样本，而第二天，在基线血液采样后，受试者被给予DSWST而不是水。在基线和给水或DSWST后采集血液样本，检查红细胞变形能力。结果与摄入DSWST后的基线相比，延长指数在2 h后显著增加(P = 0.009)。然而，在饮水后，没有观察到显著差异。在比较DSWST与水对红细胞变形能力的百分比变化时，我们发现，在给药2 h后，与水相比，DSWST显著改善了红细胞变形能力(P <0.001)。结论dswst对正常人红细胞变形能力有短暂性影响。

{"title":"Effect of the herbal medicine Danggui Sini plus Wuzhuyu Shengjiang Tang on erythrocyte deformability in normal subjects: a cross-over trial","authors":"JiEun Lee , Seung-Yeon Cho , Seong-Uk Park , Woo-Sang Jung , Sang-Kwan Moon , Jung-Mi Park , Chang-Nam Ko , Ki-Ho Cho , Seungwon Kwon","doi":"10.1016/S0254-6272(18)30917-8","DOIUrl":"10.1016/S0254-6272(18)30917-8","url":null,"abstract":"<div><h3>OBJECTIVE</h3><p>To show whether Danggui Sini plus Wuzhuyu Shengjiang Tang (DSWST) has any transient effect on erythrocyte deformability in normal subjects.</p></div><div><h3>METHODS</h3><p>A total of 25 subjects [mean age (27.8 ± 1.8) years] was enrolled in this study. The study was designed as a cross-over trial in which the subjects took part for 2 d. On the first day, blood samples were collected at baseline and 1-2 h after administration of water, whereas, on the second day, instead of water, the subjects were administered DSWST after the baseline blood sampling. The blood samples collected at baseline and after the administration water or DSWST, were examined for erythrocyte deformability.</p></div><div><h3>RESULTS</h3><p>The elongation index increased significantly after 2 h (<em>P</em> = 0.009) compared to the baseline after DSWST intake. However, after water intake, there was no significant difference observed. When comparing the percent change of erythrocyte deformability between DSWST and water, we found that after 2 h of administration, DSWST improved erythrocyte deformability significantly compared to water (<em>P</em> < 0.001).</p></div><div><h3>CONCLUSION</h3><p>DSWST has a transient effect on erythrocyte deformability in normal subjects.</p></div>","PeriodicalId":17513,"journal":{"name":"Journal of Traditional Chinese Medicine","volume":"38 5","pages":"Pages 769-772"},"PeriodicalIF":2.6,"publicationDate":"2018-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/S0254-6272(18)30917-8","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"47814723","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Effect of Ginkgo biloba leaf extract on cerebral cortex amino acid levels in cerebral ischemia model rats 银杏叶提取物对脑缺血模型大鼠大脑皮层氨基酸水平的影响

IF 2.6 4区医学 Q2 INTEGRATIVE & COMPLEMENTARY MEDICINE

Journal of Traditional Chinese Medicine

Pub Date : 2018-10-01 DOI: 10.1016/S0254-6272(18)30906-3

Cui Yiran , Wu Hongwei , Liu Mengting , Qin Haijiao , Liu Xin , Yang Hongjun

OBJECTIVE

To investigate the effect of Ginkgo biloba leaf extract on amino acid levels in the cerebral cortex of cerebral ischemia model rats induced by middle cerebral artery occlusion (MCAO).

METHODS

A rat model of cerebral ischemia was established by MCAO. Male rats were divided into a negative control group (Control), a sham-operated group (Sham), an ischemic group (MCAO), and an ischemic group treated with Ginkgo biloba leaf extract (MCAO_D). All groups were divided into two subgroups with occlusion times of 12 and 24 h, respectively. The levels of 18 endogenous amino acids in the cerebral cortex were quantified by triple quadrupole-liquid chromatography-mass spectrometry.

RESULTS

Compared with the MCAO group, behavioral performance, neurological deficit score, and cerebral infarct volume were significantly improved in the MCAO_D group (P < 0.05, P < 0.01). Compared with the sham group, the levels of 17 amino acids in the cerebral cortex were markedly changed in the MCAO group. The levels of Alanine (Ala), Isoleucine (Ile), Glutamic acid (Glu), Serine (Ser), Valine (Val), Phenylalanine (Phe), Proline (Pro), Threonine (Thr), Lysine (Lys), Tyrosine (Tyr), Hydroxyproline (Hyp), Arginine (Arg), Leucine (Leu), Tryptophan (Trp), and Glycine (Gly) were increased (P < 0.001, P < 0.05), while levels of Gln and Tau were decreased (P < 0.001, P < 0.05). Compared with the MCAO group, Ginkgo biloba extract treatment in the MCAO_D group significantly down-regulated the levels of 11 amino acids, especially those of Arg, Thr, and Ser in 12 or 24 h.

CONCLUSION

Injection of Ginkgo biloba leaf extract has a therapeutic effect on model rats with MCAO-induced cerebral ischemia by acting on amino acids in the cerebral cortex. This effect might be associated with the regulation of amino acid metabolism in the cerebral cortex.

目的探讨银杏叶提取物对大脑中动脉闭塞(MCAO)致脑缺血模型大鼠大脑皮层氨基酸水平的影响。方法采用MCAO建立大鼠脑缺血模型。将雄性大鼠分为阴性对照组(control)、假手术组(Sham)、缺血组(MCAO)和银杏叶提取物缺血组(MCAO_D)。各组均分为2个亚组，阻断时间分别为12和24 h。采用三重四极柱-液相色谱-质谱联用法定量测定大鼠大脑皮层中18种内源氨基酸的含量。结果与MCAO组比较，MCAO_D组大鼠行为表现、神经功能缺损评分、脑梗死体积均显著改善(P <0.05, P <0.01)。与假手术组比较，MCAO组大鼠大脑皮层17种氨基酸水平明显改变。丙氨酸(Ala)、异亮氨酸(Ile)、谷氨酸(Glu)、丝氨酸(Ser)、缬氨酸(Val)、苯丙氨酸(Phe)、脯氨酸(Pro)、苏氨酸(Thr)、赖氨酸(Lys)、酪氨酸(Tyr)、羟脯氨酸(Hyp)、精氨酸(Arg)、亮氨酸(Leu)、色氨酸(Trp)和甘氨酸(Gly)水平均升高(P <0.001, P <0.05)， Gln和Tau水平降低(P <0.001, P <0.05)。与MCAO组比较，MCAO_D组银杏叶提取物在12 h和24 h显著下调了11种氨基酸的水平，尤其是精氨酸、苏氨酸和丝氨酸的水平。结论注射银杏叶提取物通过作用于大脑皮层的氨基酸对MCAO诱导的脑缺血模型大鼠有治疗作用。这种作用可能与调节大脑皮层的氨基酸代谢有关。

{"title":"Effect of Ginkgo biloba leaf extract on cerebral cortex amino acid levels in cerebral ischemia model rats","authors":"Cui Yiran , Wu Hongwei , Liu Mengting , Qin Haijiao , Liu Xin , Yang Hongjun","doi":"10.1016/S0254-6272(18)30906-3","DOIUrl":"10.1016/S0254-6272(18)30906-3","url":null,"abstract":"<div><h3>OBJECTIVE</h3><p>To investigate the effect of Ginkgo biloba leaf extract on amino acid levels in the cerebral cortex of cerebral ischemia model rats induced by middle cerebral artery occlusion (MCAO).</p></div><div><h3>METHODS</h3><p>A rat model of cerebral ischemia was established by MCAO. Male rats were divided into a negative control group (Control), a sham-operated group (Sham), an ischemic group (MCAO), and an ischemic group treated with Ginkgo biloba leaf extract (MCAO_D). All groups were divided into two subgroups with occlusion times of 12 and 24 h, respectively. The levels of 18 endogenous amino acids in the cerebral cortex were quantified by triple quadrupole-liquid chromatography-mass spectrometry.</p></div><div><h3>RESULTS</h3><p>Compared with the MCAO group, behavioral performance, neurological deficit score, and cerebral infarct volume were significantly improved in the MCAO_D group (<em>P</em> < 0.05, <em>P</em> < 0.01). Compared with the sham group, the levels of 17 amino acids in the cerebral cortex were markedly changed in the MCAO group. The levels of Alanine (Ala), Isoleucine (Ile), Glutamic acid (Glu), Serine (Ser), Valine (Val), Phenylalanine (Phe), Proline (Pro), Threonine (Thr), Lysine (Lys), Tyrosine (Tyr), Hydroxyproline (Hyp), Arginine (Arg), Leucine (Leu), Tryptophan (Trp), and Glycine (Gly) were increased (<em>P</em> < 0.001, <em>P</em> < 0.05), while levels of Gln and Tau were decreased (<em>P</em> < 0.001, <em>P</em> < 0.05). Compared with the MCAO group, Ginkgo biloba extract treatment in the MCAO_D group significantly down-regulated the levels of 11 amino acids, especially those of Arg, Thr, and Ser in 12 or 24 h.</p></div><div><h3>CONCLUSION</h3><p>Injection of Ginkgo biloba leaf extract has a therapeutic effect on model rats with MCAO-induced cerebral ischemia by acting on amino acids in the cerebral cortex. This effect might be associated with the regulation of amino acid metabolism in the cerebral cortex.</p></div>","PeriodicalId":17513,"journal":{"name":"Journal of Traditional Chinese Medicine","volume":"38 5","pages":"Pages 676-684"},"PeriodicalIF":2.6,"publicationDate":"2018-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/S0254-6272(18)30906-3","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41620552","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Effects of Ziyin Jianghuo Ningxin decoction plus dehydroepiandrosterone and femoston in treatment of patients with menopausal symptoms 紫饮降活宁心汤联合脱氢表雄酮、铁素治疗绝经期症状的疗效观察

IF 2.6 4区医学 Q2 INTEGRATIVE & COMPLEMENTARY MEDICINE

Journal of Traditional Chinese Medicine

Pub Date : 2018-10-01 DOI: 10.1016/S0254-6272(18)30920-8

Lin Jing , Zhu Jun , Tian Fubo , Chen Lanting , Zhang Yang , Wang Yan , Wang Mingyan , Hans-Jürgen Gober , Li Dajin , Wang Ling

OBJECTIVE

To determine the therapeutic effect of Ziyin Jianghuo Ningxin Decoction (ZYJHNXD) plus dehydroepiandrosterone (DHEA) and menopausal hormone therapy (MHT) in patients suffering from menopausal symptoms identified as, in terms of Traditional Chinese Medicine, symptom pattern of Yin deficiency with hyperactive fire.

METHODS

Totally 180 postmenopausal women aged 40 to 60 years were assigned into four groups and accepted femoston, femoston with ZYJHNXD, femoston with DHEA, femoston with ZYJHNXD and DHEA therapies, respectively, for three months. Common questionnaire-based measure instruments included modified Kupperman index (MKI), Hamilton Rating Scale for Anxiety (HAMA), and Hamilton Rating Scale for Depression (HAMD). Follicle-stimulating hormone (FSH), luteinizing hormone (LH), estradiol (E2), 5-hydroxyindole-3-acetic acid (5-HIAA), norepinephrine (NE), dopamine (DA), bone mineral density (BMD), and sleep quality were evaluated before and three months after the treatments.

RESULTS

In all four groups, the scores of MKI, HAMA, HAMD and the levels of FSH, LH decreased significantly (P < 0.05) after the treatment, while the levels of E2, 5-HIAA, NE, and DA showed obvious elevation (P < 0.05). The group receiving ZYJHNXD and DHEA combined with femoston had superiority in the preservation of bone mineral density and improvement of total sleep time and nighttime sleep time over the other three groups.

CONCLUSION

ZYJHNXD and DHEA combined with MHT therapy have a favorable outcome in managing menopausal symptoms, restoring hormone levels, preventing skeletal rarefaction or osteoporosis, and improving sleep quality for postmenopausal women.

目的观察紫饮降活宁心汤联合脱氢表雄酮(DHEA)和更年期激素治疗(MHT)对中医认为阴虚火旺型绝经期症状的疗效。方法将180名年龄在40 ~ 60岁的绝经后妇女分为4组，分别接受激素、激素联合ZYJHNXD、激素联合脱氢表雄酮、激素联合ZYJHNXD和脱氢表雄酮治疗，疗程3个月。常见的基于问卷的测量工具包括改良的Kupperman指数(MKI)、汉密尔顿焦虑量表(HAMA)和汉密尔顿抑郁量表(HAMD)。分别于治疗前和治疗后3个月测定促卵泡激素(FSH)、促黄体生成素(LH)、雌二醇(E2)、5-羟基吲哚-3-乙酸(5-HIAA)、去甲肾上腺素(NE)、多巴胺(DA)、骨密度(BMD)和睡眠质量。结果4组患者MKI、HAMA、HAMD评分及FSH、LH水平均显著降低(P <0.05)， E2、5-HIAA、NE、DA水平明显升高(P <0.05)。ZYJHNXD和DHEA联合铁素组在保持骨密度、改善总睡眠时间和夜间睡眠时间方面优于其他三组。结论zyjhnxd联合DHEA联合MHT治疗绝经后妇女在控制绝经期症状、恢复激素水平、预防骨骼疏松或骨质疏松、改善睡眠质量等方面效果良好。

{"title":"Effects of Ziyin Jianghuo Ningxin decoction plus dehydroepiandrosterone and femoston in treatment of patients with menopausal symptoms","authors":"Lin Jing , Zhu Jun , Tian Fubo , Chen Lanting , Zhang Yang , Wang Yan , Wang Mingyan , Hans-Jürgen Gober , Li Dajin , Wang Ling","doi":"10.1016/S0254-6272(18)30920-8","DOIUrl":"10.1016/S0254-6272(18)30920-8","url":null,"abstract":"<div><h3>OBJECTIVE</h3><p>To determine the therapeutic effect of Ziyin Jianghuo Ningxin Decoction (ZYJHNXD) plus dehydroepiandrosterone (DHEA) and menopausal hormone therapy (MHT) in patients suffering from menopausal symptoms identified as, in terms of Traditional Chinese Medicine, symptom pattern of <em>Yin</em> deficiency with hyperactive fire.</p></div><div><h3>METHODS</h3><p>Totally 180 postmenopausal women aged 40 to 60 years were assigned into four groups and accepted femoston, femoston with ZYJHNXD, femoston with DHEA, femoston with ZYJHNXD and DHEA therapies, respectively, for three months. Common questionnaire-based measure instruments included modified Kupperman index (MKI), Hamilton Rating Scale for Anxiety (HAMA), and Hamilton Rating Scale for Depression (HAMD). Follicle-stimulating hormone (FSH), luteinizing hormone (LH), estradiol (E2), 5-hydroxyindole-3-acetic acid (5-HIAA), norepinephrine (NE), dopamine (DA), bone mineral density (BMD), and sleep quality were evaluated before and three months after the treatments.</p></div><div><h3>RESULTS</h3><p>In all four groups, the scores of MKI, HAMA, HAMD and the levels of FSH, LH decreased significantly (<em>P</em> < 0.05) after the treatment, while the levels of E2, 5-HIAA, NE, and DA showed obvious elevation (<em>P</em> < 0.05). The group receiving ZYJHNXD and DHEA combined with femoston had superiority in the preservation of bone mineral density and improvement of total sleep time and nighttime sleep time over the other three groups.</p></div><div><h3>CONCLUSION</h3><p>ZYJHNXD and DHEA combined with MHT therapy have a favorable outcome in managing menopausal symptoms, restoring hormone levels, preventing skeletal rarefaction or osteoporosis, and improving sleep quality for postmenopausal women.</p></div>","PeriodicalId":17513,"journal":{"name":"Journal of Traditional Chinese Medicine","volume":"38 5","pages":"Pages 787-796"},"PeriodicalIF":2.6,"publicationDate":"2018-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/S0254-6272(18)30920-8","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41914128","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 4

Protective effects of effective ingredients of Danshen (Radix Salviae Miltiorrhizae) and Honghua (Flos Carthami) compatibility after rat hippocampal neurons induced by hypoxia injury 丹参、红花有效成分对缺氧损伤大鼠海马神经元的保护作用

IF 2.6 4区医学 Q2 INTEGRATIVE & COMPLEMENTARY MEDICINE

Journal of Traditional Chinese Medicine

Pub Date : 2018-10-01 DOI: 10.1016/S0254-6272(18)30907-5

Yu Li , Wan Haofang , Jin Weifeng , Yang Jiehong , Li Chang , Dai Liuling , Ge Lijun , Zhou Huifen , Wan Haitong , He Yu

OBJECTIVE

To investigate the effective ingredients of Danshen (Radix Salviae Miltiorrhizae) and Honghua (Flos Carthami) (Danhong) on protective properties towards neonatal rat hippocampal neurons under hypoxia condition.

METHODS

Primary culture of neonatal rat hippocampal neurons was used to model hypoxia damage on the hippocampus. Methyl thiazolyl tetrazolium (MTT) assay and preliminary experiments were conducted to identify the four effective ingredients of Danhong, along with the injection of positive drug onto hippocampal neurons at a non-toxic dosage level. The cultured cells were randomly divided into 12 groups: the normal group, model group, positive drug control group and nine compatibility groups of the four effective ingredients. Different test methods were applied to determine lactate dehydrogenasein (LDH), total superoxide dismutase (T-SOD), malondialdehyde (MDA), 6-keto prostaglandin F_la (6-keto-PGF_1a), thromboxane B₂ (TXB₂), mitochondrial membrane potential (MMP), free calcium ions concentration ([Ca²⁺]i) and early onset cell apoptosis.

RESULTS

Different compatibility groups could inhibit the content of LDH and intracellular calcium overload, increase activity in T-SOD, decrease level of MDA and TXB₂, improve level of 6-keto-PGF_1a and MMP, and prevent the early onset cell apoptosis.

CONCLUSION

The compatibility of four effective ingredients of Danhong had protective effect toward hippocampi hypoxia. The mechanism might be related to inhibit oxidative stress damage and cell apoptosis, resist thrombosis, and reduce the intracellular calcium ion of overload.

目的探讨丹参、红花对缺氧条件下新生大鼠海马神经元的保护作用。方法采用新生大鼠海马神经元原代培养法模拟海马缺氧损伤。采用甲基噻唑四氮唑(MTT)法和初步实验鉴定丹红的四种有效成分，并在无毒剂量水平下向海马神经元注射阳性药物。将培养细胞随机分为12组:正常组、模型组、阳性药物对照组和4种有效成分的9个配伍组。采用不同的检测方法检测乳酸脱氢酶(LDH)、总超氧化物歧化酶(T-SOD)、丙二醛(MDA)、6-酮前列腺素(6-酮- pgf1a)、血栓素B2 (TXB2)、线粒体膜电位(MMP)、游离钙离子浓度([Ca2+]i)和早发性细胞凋亡。结果不同配型组均能抑制LDH含量和细胞内钙超载，提高T-SOD活性，降低MDA和TXB2水平，提高6-酮- pgf1a和MMP水平，防止早发性细胞凋亡。结论丹红四种有效成分配伍对海马缺氧有保护作用。其机制可能与抑制氧化应激损伤和细胞凋亡、抗血栓形成、减少细胞内钙离子过载有关。

{"title":"Protective effects of effective ingredients of Danshen (Radix Salviae Miltiorrhizae) and Honghua (Flos Carthami) compatibility after rat hippocampal neurons induced by hypoxia injury","authors":"Yu Li , Wan Haofang , Jin Weifeng , Yang Jiehong , Li Chang , Dai Liuling , Ge Lijun , Zhou Huifen , Wan Haitong , He Yu","doi":"10.1016/S0254-6272(18)30907-5","DOIUrl":"10.1016/S0254-6272(18)30907-5","url":null,"abstract":"<div><h3>OBJECTIVE</h3><p>To investigate the effective ingredients of Danshen (<em>Radix Salviae Miltiorrhizae</em>) and Honghua (<em>Flos Carthami</em>) (Danhong) on protective properties towards neonatal rat hippocampal neurons under hypoxia condition.</p></div><div><h3>METHODS</h3><p>Primary culture of neonatal rat hippocampal neurons was used to model hypoxia damage on the hippocampus. Methyl thiazolyl tetrazolium (MTT) assay and preliminary experiments were conducted to identify the four effective ingredients of Danhong, along with the injection of positive drug onto hippocampal neurons at a non-toxic dosage level. The cultured cells were randomly divided into 12 groups: the normal group, model group, positive drug control group and nine compatibility groups of the four effective ingredients. Different test methods were applied to determine lactate dehydrogenasein (LDH), total superoxide dismutase (T-SOD), malondialdehyde (MDA), 6-keto prostaglandin F<sub>la</sub> (6-keto-PGF<sub>1a</sub>), thromboxane B<sub>2</sub> (TXB<sub>2</sub>), mitochondrial membrane potential (MMP), free calcium ions concentration ([Ca<sup>2+</sup>]i) and early onset cell apoptosis.</p></div><div><h3>RESULTS</h3><p>Different compatibility groups could inhibit the content of LDH and intracellular calcium overload, increase activity in T-SOD, decrease level of MDA and TXB<sub>2</sub>, improve level of 6-keto-PGF<sub>1a</sub> and MMP, and prevent the early onset cell apoptosis.</p></div><div><h3>CONCLUSION</h3><p>The compatibility of four effective ingredients of Danhong had protective effect toward hippocampi hypoxia. The mechanism might be related to inhibit oxidative stress damage and cell apoptosis, resist thrombosis, and reduce the intracellular calcium ion of overload.</p></div>","PeriodicalId":17513,"journal":{"name":"Journal of Traditional Chinese Medicine","volume":"38 5","pages":"Pages 685-697"},"PeriodicalIF":2.6,"publicationDate":"2018-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/S0254-6272(18)30907-5","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"91448411","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 8

Mixture prepared with Chinese medicines in terms of releasing exterior cold and clearing interior heat: its efficacy and safety in patients with seasonal influenza-a randomized controlled trial 中药复方外冷清热对季节性流感患者的疗效及安全性——随机对照试验

IF 2.6 4区医学 Q2 INTEGRATIVE & COMPLEMENTARY MEDICINE

Journal of Traditional Chinese Medicine

Pub Date : 2018-10-01 DOI: 10.1016/S0254-6272(18)30914-2

Wu Zhisong , Li Guodong , Wei Wan , Liu Zhaoheng , Wang Wei , Ma Ruihong , Liu Wenhong , Ma Jiaju , Wang Yuguang , Jiao Yang

OBJECTIVE

To assess the efficacy and safety in 105 patients with seasonal Influenza in Beijing, the mixture prepared with Chinese medicines follows the treatment regimen of releasing exterior cold and clearing interior heat.

METHODS

Total 330 patients with seasonal influenza were enrolled and randomly and averagely divided into the Chinese herbal medicine, the western medicine and the Chinese patent medicine group. They were treated with Chinese medicine Oseltamivir Phosphate Capsules and the Scattering Wind and Resolving Toxins Capsules. The main efficacy indicators were the antifebrile onset time and recovery time of body temperature. The efficacy and safety of the mixture was scientifically evaluated. Comparisons of several variables were analyzed.

RESULTS

Median antifebrile onset time of the Chinese herbal medicine group was significantly shorter than the western medicine group (P < 0.05) and the Chinese patent medicine group (P < 0.05). The median antifebrile recovery time of the Chinese herbal medicine group was significantly shorter than the Chinese patent medicine group (P < 0.05). The groups evaluated by TCM symptom pattern effect, both the Chinese herbal medicine group and Western Medicine group were better than the Chinese patent medicine group (P < 0.05). The disappearance rate of main symptoms and some minor symptom patterns of the Chinese herbal medicine group were higher than the other 2 groups.

CONCLUSION

The mixture of releasing exterior cold and clearing interior heat could significantly shorten the fever time with safety.

目的观察中药配伍治疗北京地区105例季节性流感患者的疗效和安全性，治疗方案为外寒清热。方法将330例季节性流感患者随机平均分为中草药组、西药组和中成药组。采用中药磷酸奥司他韦胶囊和散风解毒胶囊治疗。主要疗效指标为退热起效时间和体温恢复时间。科学评价了合剂的有效性和安全性。对几个变量进行了比较分析。结果中草药组抗热起效时间中位数显著短于西药组(P <0.05)，中成药组(P <0.05)。中草药组的中位解热恢复时间显著短于中成药组(P <0.05)。以中医证型疗效评价各组，中草药组和西药组均优于中成药组(P <0.05)。中草药治疗组的主要症状及部分次要症状消失率均高于其他两组。结论外冷清热混合使用能明显缩短发热时间，且安全。

{"title":"Mixture prepared with Chinese medicines in terms of releasing exterior cold and clearing interior heat: its efficacy and safety in patients with seasonal influenza-a randomized controlled trial","authors":"Wu Zhisong , Li Guodong , Wei Wan , Liu Zhaoheng , Wang Wei , Ma Ruihong , Liu Wenhong , Ma Jiaju , Wang Yuguang , Jiao Yang","doi":"10.1016/S0254-6272(18)30914-2","DOIUrl":"10.1016/S0254-6272(18)30914-2","url":null,"abstract":"<div><h3>OBJECTIVE</h3><p>To assess the efficacy and safety in 105 patients with seasonal Influenza in Beijing, the mixture prepared with Chinese medicines follows the treatment regimen of releasing exterior cold and clearing interior heat.</p></div><div><h3>METHODS</h3><p>Total 330 patients with seasonal influenza were enrolled and randomly and averagely divided into the Chinese herbal medicine, the western medicine and the Chinese patent medicine group. They were treated with Chinese medicine Oseltamivir Phosphate Capsules and the Scattering Wind and Resolving Toxins Capsules. The main efficacy indicators were the antifebrile onset time and recovery time of body temperature. The efficacy and safety of the mixture was scientifically evaluated. Comparisons of several variables were analyzed.</p></div><div><h3>RESULTS</h3><p>Median antifebrile onset time of the Chinese herbal medicine group was significantly shorter than the western medicine group (<em>P</em> < 0.05) and the Chinese patent medicine group (<em>P</em> < 0.05). The median antifebrile recovery time of the Chinese herbal medicine group was significantly shorter than the Chinese patent medicine group (<em>P</em> < 0.05). The groups evaluated by TCM symptom pattern effect, both the Chinese herbal medicine group and Western Medicine group were better than the Chinese patent medicine group (<em>P</em> < 0.05). The disappearance rate of main symptoms and some minor symptom patterns of the Chinese herbal medicine group were higher than the other 2 groups.</p></div><div><h3>CONCLUSION</h3><p>The mixture of releasing exterior cold and clearing interior heat could significantly shorten the fever time with safety.</p></div>","PeriodicalId":17513,"journal":{"name":"Journal of Traditional Chinese Medicine","volume":"38 5","pages":"Pages 746-753"},"PeriodicalIF":2.6,"publicationDate":"2018-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/S0254-6272(18)30914-2","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"85192569","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 2

Effectiveness of ZiYin Xiehuo granules and Zishen Qinggan granules on partial precocious puberty in girls: a multicenter, randomized, single-blind, controlled trial 紫阴泻活颗粒与紫肾清肝颗粒治疗女童部分性早熟的疗效:一项多中心、随机、单盲、对照试验

IF 2.6 4区医学 Q2 INTEGRATIVE & COMPLEMENTARY MEDICINE

Journal of Traditional Chinese Medicine

Pub Date : 2018-10-01 DOI: 10.1016/S0254-6272(18)30913-0

Sun Wen , Han Xinghui , Wang Yonghong , Yu Jian , Yan Weili , Zhao Jun , Chen Weibin , Xue Zheng

OBJECTIVE

To evaluate the effect of ZiYin Xiehuo granules (ZYXH) and Zishen Qinggan granules (ZSQG) on partial precocious puberty (PPP).

METHODS

This was a multicenter, randomized, single-blind, positive-controlled trial. A total of 143 patients were assigned to either the ZYXH group or the ZSQG group using a random number table. The ZYXH group received ZYXH three times daily for 6 months and the ZSQG group received ZSQG three times daily for 6 months. Mammary nucleus diameter; the results of uterus, ovarian, and maximum follicle measures; and Chinese medicine symptom pattern scores were compared at baseline and after 3 months and 6 months of treatment.

RESULTS

After 3 months’ treatment, there were no significant differences between the two groups in mammary nucleus index changes (left 3.4 ± 3.1 vs 3.5 ± 3.1, P = 0.790; right 3.0 ± 2.9 vs 3.6 ± 3.0, P = 0.719). The uterine volume in the ZYXH group was smaller than that in the ZSQG group (2.1 ± 1.6 vs 2.6 ± 2.2, P = 0.006). There were no significant between-group differences in ovarian volume and maximum follicular diameter on either side (ovarian volume: left 1.2 ± 0.7 vs 1.3 ± 0.6, P = 0.8; right 1.2 ± 0.7 vs 1.4 ± 1.1, P = 0.984; maximum follicular diameter: left 3.9 ± 1.7 vs 3.5 ± 2.2, P = 0.158; right 3.5 ± 1.7 vs 3.9 ± 2.1, P = 0.314).

CONCLUSION

ZYXH granules and ZSQG granules both affected the size of the mammary nucleus in girls with PPP, and improved Chinese medicine symptom patterns. ZYXH granules showed slight advantages over ZSQG granules in terms of the decrease in the size of the uterus, ovaries, and ovarian follicles.

目的评价紫饮泻活颗粒(ZYXH)与紫肾清肝颗粒(ZSQG)治疗部分性性早熟(PPP)的疗效。方法多中心、随机、单盲、正对照试验。使用随机数字表将143例患者分配到ZYXH组或ZSQG组。ZYXH组给予ZYXH每日3次，连续6个月;ZSQG组给予ZSQG每日3次，连续6个月。乳核直径;子宫、卵巢和最大卵泡测量结果;比较两组患者治疗3个月、6个月时的中医症状分型评分。结果治疗3个月后，两组乳腺核指数变化差异无统计学意义(左3.4±3.1 vs 3.5±3.1,P = 0.790;右侧3.0±2.9 vs 3.6±3.0,P = 0.719)。ZYXH组子宫体积小于ZSQG组(2.1±1.6 vs 2.6±2.2,P = 0.006)。双侧卵巢体积和最大卵泡直径组间差异无统计学意义(左侧卵巢体积:1.2±0.7 vs 1.3±0.6,P = 0.8;右侧1.2±0.7 vs 1.4±1.1,P = 0.984;最大卵泡直径:左侧3.9±1.7 vs 3.5±2.2,P = 0.158;右3.5±1.7 vs 3.9±2.1,P = 0.314)。结论zyxh颗粒和ZSQG颗粒均能影响PPP女孩乳腺核的大小，改善其中医证型。ZYXH颗粒在子宫、卵巢和卵泡大小的减小方面比ZSQG颗粒略有优势。

{"title":"Effectiveness of ZiYin Xiehuo granules and Zishen Qinggan granules on partial precocious puberty in girls: a multicenter, randomized, single-blind, controlled trial","authors":"Sun Wen , Han Xinghui , Wang Yonghong , Yu Jian , Yan Weili , Zhao Jun , Chen Weibin , Xue Zheng","doi":"10.1016/S0254-6272(18)30913-0","DOIUrl":"10.1016/S0254-6272(18)30913-0","url":null,"abstract":"<div><h3>OBJECTIVE</h3><p>To evaluate the effect of ZiYin Xiehuo granules (ZYXH) and Zishen Qinggan granules (ZSQG) on partial precocious puberty (PPP).</p></div><div><h3>METHODS</h3><p>This was a multicenter, randomized, single-blind, positive-controlled trial. A total of 143 patients were assigned to either the ZYXH group or the ZSQG group using a random number table. The ZYXH group received ZYXH three times daily for 6 months and the ZSQG group received ZSQG three times daily for 6 months. Mammary nucleus diameter; the results of uterus, ovarian, and maximum follicle measures; and Chinese medicine symptom pattern scores were compared at baseline and after 3 months and 6 months of treatment.</p></div><div><h3>RESULTS</h3><p>After 3 months’ treatment, there were no significant differences between the two groups in mammary nucleus index changes (left 3.4 ± 3.1 <em>vs</em> 3.5 ± 3.1, <em>P</em> = 0.790; right 3.0 ± 2.9 <em>vs</em> 3.6 ± 3.0, <em>P</em> = 0.719). The uterine volume in the ZYXH group was smaller than that in the ZSQG group (2.1 ± 1.6 <em>vs</em> 2.6 ± 2.2, <em>P</em> = 0.006). There were no significant between-group differences in ovarian volume and maximum follicular diameter on either side (ovarian volume: left 1.2 ± 0.7 <em>vs</em> 1.3 ± 0.6, <em>P</em> = 0.8; right 1.2 ± 0.7 <em>vs</em> 1.4 ± 1.1, <em>P</em> = 0.984; maximum follicular diameter: left 3.9 ± 1.7 <em>vs</em> 3.5 ± 2.2, <em>P</em> = 0.158; right 3.5 ± 1.7 <em>vs</em> 3.9 ± 2.1, <em>P</em> = 0.314).</p></div><div><h3>CONCLUSION</h3><p>ZYXH granules and ZSQG granules both affected the size of the mammary nucleus in girls with PPP, and improved Chinese medicine symptom patterns. ZYXH granules showed slight advantages over ZSQG granules in terms of the decrease in the size of the uterus, ovaries, and ovarian follicles.</p></div>","PeriodicalId":17513,"journal":{"name":"Journal of Traditional Chinese Medicine","volume":"38 5","pages":"Pages 740-745"},"PeriodicalIF":2.6,"publicationDate":"2018-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/S0254-6272(18)30913-0","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"49601723","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Pricking and penetrating moxibustion therapy in patients with refractory insomnia: a randomized and controlled clinical trial 刺穿灸治疗难治性失眠:一项随机对照临床试验

IF 2.6 4区医学 Q2 INTEGRATIVE & COMPLEMENTARY MEDICINE

Journal of Traditional Chinese Medicine

Pub Date : 2018-10-01 DOI: 10.1016/S0254-6272(18)30915-4

Chen Yan , Gao Xiyan , Sun Cuiying

OBJECTIVE

To observe the clinical effect of pricking and penetrating moxibustion therapy on refractory insomnia.

METHODS

Totally 60 subjects were randomly divided into treatment group and control group, 30 in each group. The treatment group was treated with pricking and penetrating moxibustion therapy, the control group with penetrating moxibustion therapy, the treatment was given once every day, two groups of patients were treated 20 times, compared the efficacy, pinsburgh sleep quality index (PSQI) and Traditional Chinese Medicine symptom, safety evaluation between the two groups.

RESULTS

Compared with 10, 20 times after the treatment, the effective rate of the treatment group (93.3%) higher than that of the control group (80.0%) (P < 0.05); Compared with before treatment, 20 times after treatment, the PSQI and TCM symptom pattern scores of the two groups were significantly decreased (P < 0.01); Compared with 10, 20 times after the treatment, the treatment group are better than the control group in improving the total score and the integral of PSQI (P < 0.05 or P < 0.01); The treatment group are better than the control group in improving the TCM symptom pattern score, difficulty in going to sleep, palpitation, amnesia, fidget, sweating, impaired concentration (P < 0.01 or P < 0.05).

CONCLUSION

The pricking and penetrating moxibustion therapy is safe and effective in the treatment of refractory insomnia and the treatment can improve the curative effect of the therapy that uses penetrating moxibustion only.

目的观察刺穿灸法治疗难治性失眠的临床疗效。方法将60例患者随机分为治疗组和对照组，每组30例。治疗组采用刺穿灸法治疗，对照组采用刺穿灸法治疗，每天给予1次治疗，两组患者共治疗20次，比较两组患者的疗效、睡眠质量指数(PSQI)及中医症状、安全性评价。结果与治疗后10、20次相比，治疗组有效率(93.3%)高于对照组(80.0%)(P <0.05);与治疗前、治疗后20倍比较，两组患者PSQI及中医证候分均显著降低(P <0.01);与治疗后10、20次比较，治疗组在改善总评分和PSQI积分(P <0.05或P <0.01);治疗组在改善中医证型评分、入睡困难、心悸、健忘、坐立不安、多汗、注意力不集中等方面均优于对照组(P <0.01或P <0.05)。结论刺穿灸法治疗难治性失眠安全有效，可提高单纯刺穿灸法的疗效。

{"title":"Pricking and penetrating moxibustion therapy in patients with refractory insomnia: a randomized and controlled clinical trial","authors":"Chen Yan , Gao Xiyan , Sun Cuiying","doi":"10.1016/S0254-6272(18)30915-4","DOIUrl":"10.1016/S0254-6272(18)30915-4","url":null,"abstract":"<div><h3>OBJECTIVE</h3><p>To observe the clinical effect of pricking and penetrating moxibustion therapy on refractory insomnia.</p></div><div><h3>METHODS</h3><p>Totally 60 subjects were randomly divided into treatment group and control group, 30 in each group. The treatment group was treated with pricking and penetrating moxibustion therapy, the control group with penetrating moxibustion therapy, the treatment was given once every day, two groups of patients were treated 20 times, compared the efficacy, pinsburgh sleep quality index (PSQI) and Traditional Chinese Medicine symptom, safety evaluation between the two groups.</p></div><div><h3>RESULTS</h3><p>Compared with 10, 20 times after the treatment, the effective rate of the treatment group (93.3%) higher than that of the control group (80.0%) (<em>P</em> < 0.05); Compared with before treatment, 20 times after treatment, the PSQI and TCM symptom pattern scores of the two groups were significantly decreased (<em>P</em> < 0.01); Compared with 10, 20 times after the treatment, the treatment group are better than the control group in improving the total score and the integral of PSQI (<em>P</em> < 0.05 or <em>P</em> < 0.01); The treatment group are better than the control group in improving the TCM symptom pattern score, difficulty in going to sleep, palpitation, amnesia, fidget, sweating, impaired concentration (<em>P</em> < 0.01 or <em>P</em> < 0.05).</p></div><div><h3>CONCLUSION</h3><p>The pricking and penetrating moxibustion therapy is safe and effective in the treatment of refractory insomnia and the treatment can improve the curative effect of the therapy that uses penetrating moxibustion only.</p></div>","PeriodicalId":17513,"journal":{"name":"Journal of Traditional Chinese Medicine","volume":"38 5","pages":"Pages 754-762"},"PeriodicalIF":2.6,"publicationDate":"2018-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/S0254-6272(18)30915-4","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"45732269","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 2

Ethyl acetate fraction in ethanol extract from root of “Dai-Bai-Jie” (Marsdenia tenacissima): anti-tumor activity in A549 cancer cells 大白节根乙醇提取物乙酸乙酯部分:对A549癌细胞的抗肿瘤活性

IF 2.6 4区医学 Q2 INTEGRATIVE & COMPLEMENTARY MEDICINE

Journal of Traditional Chinese Medicine

Pub Date : 2018-10-01 DOI: 10.1016/S0254-6272(18)30905-1

Li Xiaohua , Li Haitao , Jin Lingyu , Chen Leixi , Niu Yingfeng , Guan Yanhong , Ma Xiaojun , Zhang Lixia

OBJECTIVE

To evaluate the anti-tumor activity of ethyl acetate fraction (EFA), extracted with ethanol from the root of “Dai-Bai-Jie” in A549 cancer cells and its underlying mechanism.

METHODS

“Dai-Bai-Jie” was extracted with 95% ethanol-aqueous (DBJ-1), 50% ethanol-aqueous (DBJ-2), and water (DBJ-3) by reflux method. 95% ethanol-aqueous extract was separated byethyl acetate (EFA) and n-butyl alcohol (DBJ-5), consecutively. The SRB method was used to evaluate the cytotoxic activity. Annexin V-FITC staining was applied to observe the apoptosis and analyze the cell cycle activated by EFA in A549 tumor cell. Western blot was used to detect the apoptosis/related proteins expressions. A549 tumor cellsbearing nude mice model was employed to measure the tumor volume, mice weight, and tumor inhibition ratio in order to verify the antitumor activity in vivo.

RESULTS

DBJ-1 and EFA showed better cytotoxic activity on A549 tumor cells with IC₅₀ 25 and 3.5 µg/mL, respectively. EFA can exhibit the proliferation, arrest cell cycle at G0/G1 phase, and induce apoptosis in A549 tumor cells in vitro. The mechanisms of apoptosis induced by EFA may be associated with decreasing Bcl-2 protein expression and increasing p53, Bax, Caspase-3, and Caspase-8 proteins expression. EFA also possessed significant anti-tumor efficacy in nude mice, and little toxicity was observed in the host.

CONCLUSION

EAF could induce A549 tumor cells apoptosis and G0/G1 cell cycle arrest. A549 tumor cells apoptosis induced by EAF may be associated with the decrease in the ratio of Bcl-2 and Bax mRNA levels, and increase in the expression of p53, Caspase-3, and Caspase-8 proteins.

目的研究代白节根乙醇提取乙酸乙酯部位对A549肿瘤细胞的抗肿瘤作用及其机制。方法以95%乙醇水溶液(DBJ-1)、50%乙醇水溶液(DBJ-2)、水(DBJ-3)为萃取剂，采用回流法提取“代白节”。用乙酸乙酯(EFA)和正丁醇(DBJ-5)分别对95%乙醇-水提物进行分离。采用SRB法评价其细胞毒活性。采用Annexin V-FITC染色法观察A549肿瘤细胞凋亡情况，分析EFA激活的细胞周期。Western blot检测细胞凋亡/相关蛋白的表达。采用携带A549肿瘤细胞的裸鼠模型，测定肿瘤体积、小鼠体重和肿瘤抑制率，验证其体内抗肿瘤活性。结果dbj -1和EFA对A549肿瘤细胞具有较好的细胞毒活性，IC50分别为25和3.5µg/mL。EFA能在体外诱导A549肿瘤细胞增殖，使细胞周期停留在G0/G1期，并诱导细胞凋亡。EFA诱导细胞凋亡的机制可能与降低Bcl-2蛋白表达，增加p53、Bax、Caspase-3、Caspase-8蛋白表达有关。EFA对裸鼠也有明显的抗肿瘤作用，对宿主的毒性很小。结论eaf可诱导A549肿瘤细胞凋亡，阻滞G0/G1细胞周期。EAF诱导A549肿瘤细胞凋亡可能与Bcl-2和Bax mRNA水平比值降低，p53、Caspase-3和Caspase-8蛋白表达升高有关。

{"title":"Ethyl acetate fraction in ethanol extract from root of “Dai-Bai-Jie” (Marsdenia tenacissima): anti-tumor activity in A549 cancer cells","authors":"Li Xiaohua , Li Haitao , Jin Lingyu , Chen Leixi , Niu Yingfeng , Guan Yanhong , Ma Xiaojun , Zhang Lixia","doi":"10.1016/S0254-6272(18)30905-1","DOIUrl":"10.1016/S0254-6272(18)30905-1","url":null,"abstract":"<div><h3>OBJECTIVE</h3><p>To evaluate the anti-tumor activity of ethyl acetate fraction (EFA), extracted with ethanol from the root of “Dai-Bai-Jie” in A549 cancer cells and its underlying mechanism.</p></div><div><h3>METHODS</h3><p>“Dai-Bai-Jie” was extracted with 95% ethanol-aqueous (DBJ-1), 50% ethanol-aqueous (DBJ-2), and water (DBJ-3) by reflux method. 95% ethanol-aqueous extract was separated byethyl acetate (EFA) and n-butyl alcohol (DBJ-5), consecutively. The SRB method was used to evaluate the cytotoxic activity. Annexin V-FITC staining was applied to observe the apoptosis and analyze the cell cycle activated by EFA in A549 tumor cell. Western blot was used to detect the apoptosis/related proteins expressions. A549 tumor cellsbearing nude mice model was employed to measure the tumor volume, mice weight, and tumor inhibition ratio in order to verify the antitumor activity <em>in vivo.</em></p></div><div><h3>RESULTS</h3><p>DBJ-1 and EFA showed better cytotoxic activity on A549 tumor cells with IC<sub>50</sub> 25 and 3.5 µg/mL, respectively. EFA can exhibit the proliferation, arrest cell cycle at G0/G1 phase, and induce apoptosis in A549 tumor cells <em>in vitro.</em> The mechanisms of apoptosis induced by EFA may be associated with decreasing Bcl-2 protein expression and increasing p53, Bax, Caspase-3, and Caspase-8 proteins expression. EFA also possessed significant anti-tumor efficacy in nude mice, and little toxicity was observed in the host.</p></div><div><h3>CONCLUSION</h3><p>EAF could induce A549 tumor cells apoptosis and G0/G1 cell cycle arrest. A549 tumor cells apoptosis induced by EAF may be associated with the decrease in the ratio of Bcl-2 and Bax mRNA levels, and increase in the expression of p53, Caspase-3, and Caspase-8 proteins.</p></div>","PeriodicalId":17513,"journal":{"name":"Journal of Traditional Chinese Medicine","volume":"38 5","pages":"Pages 668-675"},"PeriodicalIF":2.6,"publicationDate":"2018-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/S0254-6272(18)30905-1","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"44573953","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1