Pub Date : 2018-04-01DOI: 10.1016/j.jtcm.2018.03.004
Li Yan , Gu Jianzhong , Zhou Huamiao , Wang Hui , Zou Ying , Yang Weihong , Guo Yong
OBJECTIVE
To observe the symptom patterns (or syndromes) according to Traditional Chinese Medicine (TCM) theory in patients with various stages of colorectal cancer, and to observe the dynamic evolution process of these TCM patterns.
METHODS
A prospective and cross-sectional questionnaire-based investigation was performed. Clinical data on TCM symptom patterns in patients with colorectal cancer in the perioperative period (210 cases) and adjuvant treatment period (160 cases) were collected. EPIData 3.1 together with frequency statistics and cluster analyses were performed to identify the TCM patterns based on symptom characteristics in patients with colorectal cancer, and to assess the dynamic changes in these patterns.
RESULTS
In the perioperative period, from the first day of perioperative care to postoperative days 3, 7, and 10, the TCM pattern showed a process of dynamic change from blood deficiency to deficiency of both Qi and Yin and the pattern of dampness and hot accumulative knotting. In the adjuvant treatment period, the TCM pattern changed from Qi deficiency and Yin deficiency inner-heat with dampness to a deficiency pattern, primarily including Yin deficiency of the liver and kidney, deficiency of Qi and blood, and spleen deficiency.
CONCLUSION
Our study confirmed that variations in the dynamic evolution of TCM symptom patterns exist in patients with colorectal cancer during different treatment periods. This information is of great value in the individualized management of colorectal cancer.
{"title":"Traditional Chinese Medicine symptom patterns in patients with colorectal carcinoma","authors":"Li Yan , Gu Jianzhong , Zhou Huamiao , Wang Hui , Zou Ying , Yang Weihong , Guo Yong","doi":"10.1016/j.jtcm.2018.03.004","DOIUrl":"10.1016/j.jtcm.2018.03.004","url":null,"abstract":"<div><h3>OBJECTIVE</h3><p>To observe the symptom patterns (or syndromes) according to Traditional Chinese Medicine (TCM) theory in patients with various stages of colorectal cancer, and to observe the dynamic evolution process of these TCM patterns.</p></div><div><h3>METHODS</h3><p>A prospective and cross-sectional questionnaire-based investigation was performed. Clinical data on TCM symptom patterns in patients with colorectal cancer in the perioperative period (210 cases) and adjuvant treatment period (160 cases) were collected. EPIData 3.1 together with frequency statistics and cluster analyses were performed to identify the TCM patterns based on symptom characteristics in patients with colorectal cancer, and to assess the dynamic changes in these patterns.</p></div><div><h3>RESULTS</h3><p>In the perioperative period, from the first day of perioperative care to postoperative days 3, 7, and 10, the TCM pattern showed a process of dynamic change from blood deficiency to deficiency of both <em>Qi</em> and <em>Yin</em> and the pattern of dampness and hot accumulative knotting. In the adjuvant treatment period, the TCM pattern changed from <em>Qi</em> deficiency and <em>Yin</em> deficiency inner-heat with dampness to a deficiency pattern, primarily including <em>Yin</em> deficiency of the liver and kidney, deficiency of <em>Qi</em> and blood, and spleen deficiency.</p></div><div><h3>CONCLUSION</h3><p>Our study confirmed that variations in the dynamic evolution of TCM symptom patterns exist in patients with colorectal cancer during different treatment periods. This information is of great value in the individualized management of colorectal cancer.</p></div>","PeriodicalId":17513,"journal":{"name":"Journal of Traditional Chinese Medicine","volume":"38 2","pages":"Pages 299-308"},"PeriodicalIF":2.6,"publicationDate":"2018-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/j.jtcm.2018.03.004","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"47554241","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2018-04-01DOI: 10.1016/j.jtcm.2018.03.002
Zhao Yiming , Zheng Dongxue , Cheng Ran , Xu Xin , Lian Fengmei , Tong Xiaolin , Liu Xinmin
OBJECTIVE
To observe the efficacy and safety of different dosages of modified Zhibaidihuang decoction (MZBDD) on polycystic ovary syndrome (PCOS) hyperandrogenism (HA) patients.
METHODS
Ninety PCOS HA patients and 30 infertile patients due to tubal factor were selected. Ninety PCOS HA patients were randomly divided into three groups: low dosage group (LDG), medium dosage group (MDG) and high dosage group (HDG) and infertile patients were selected as normal control group (NCG). PCOS HA patients were treated with different dosage of MZBDD for 4 weeks. For HA patients, serum total testosterone (T), estrodial (E2), follicle stimulating hormone (FSH), luteinizing hormone (LH), prolatin (PRL), alanine aminotransferase (AST), aspartate amino transferase (AST) were determined before and after treatment, while acne scores (Rosenfield) were signed. Basal body temperatures (BBT) were asked to be measured every day. And for the else, T, E2, FSH, LH, PRL, AST, and AST were determined before treatment.
RESULTS
Totally 111 patients completed the clinical research. There were no differences among the four groups on serum T before treatment (P = 0.221). Serum T concentration of both MDG and HDG after treatment significantly were lower than that of before treatment (P = 0.039, P = 0.000), while there was no obvious difference in LDG (P = 0.829). Serum T concentration of both MDG and HDG were significantly lower than that of LDG after treatment (P = 0.048, P = 0.006). To compared with before treatment, there were no differences in Serum FSH, LH, E2, P and PRL among the three groups (LDG, MDG, HDG) (as for FSH, P = 0.136, P = 0.503, P = 0.062; as for LH, P = 0.473, P = 0.513, P = 0.096; as for E2, P = 0.206, P = 0.927, P = 0.076; as for PRL, P = 0.120, P = 0.903, P = 0.407, as for P, P = 0.308, P = 0.866, P = 0480). Acne scores of all the three groups were obviously lower than that of before treatment (P = 0.031; P = 0.033; P = 0.002). 39.5% of the patients had biphasic BBT, but there were no differences among the three groups (P = 0.510). There were no differences with ALT and AST between after and before treatment among the three groups (LDG, MDG, HDG) (as for ALT, P = 0.742, P = 0.383, P = 0.053; as for AST, P = 0.732, P = 0.519, P = 0.120).
CONCLUSION
Different dosage of MZBDD has dose-effect relationship in treating PCOS HA.
目的观察不同剂量栀白地黄汤加味治疗多囊卵巢综合征(PCOS)高雄激素症(HA)的疗效和安全性。方法选择90例PCOS HA患者和30例输卵管因素导致的不孕患者。90例PCOS HA患者随机分为低剂量组(LDG)、中剂量组(MDG)、高剂量组(HDG) 3组，选取不育患者作为正常对照组(NCG)。PCOS HA患者采用不同剂量的MZBDD治疗4周。HA患者治疗前后测定血清总睾酮(T)、雌二醇(E2)、促卵泡激素(FSH)、促黄体生成素(LH)、催乳素(PRL)、丙氨酸转氨酶(AST)、天冬氨酸转氨酶(AST)，并标记痤疮评分(Rosenfield)。要求每天测量基础体温(BBT)。治疗前检测T、E2、FSH、LH、PRL、AST、AST。结果共111例患者完成临床研究。四组患者治疗前血清T水平比较，差异无统计学意义(P = 0.221)。治疗后MDG、HDG血清T浓度均显著低于治疗前(P = 0.039, P = 0.000)， LDG无明显差异(P = 0.829)。治疗后MDG和HDG血清T浓度均显著低于LDG (P = 0.048, P = 0.006)。三组(LDG、MDG、HDG)患者血清FSH、LH、E2、P、PRL与治疗前比较，差异均无统计学意义(FSH, P = 0.136、P = 0.503、P = 0.062;LH, P = 0.473, P = 0.513, P = 0.096;E2, P = 0.206, P = 0.927, P = 0.076;PRL P = 0.120, P = 0.903, P = 0.407, P = 0.308, P = 0.866, P = 0480)。三组患者痤疮评分均显著低于治疗前(P = 0.031;P = 0.033;P = 0.002)。39.5%的患者为双期BBT，但三组间差异无统计学意义(P = 0.510)。LDG、MDG、HDG三组患者治疗前后ALT、AST差异无统计学意义(ALT, P = 0.742, P = 0.383, P = 0.053;AST, P = 0.732, P = 0.519, P = 0.120)。结论不同剂量MZBDD治疗PCOS HA有剂量效应关系。
{"title":"Dose-effect analysis of treatment by modified Zhibaidihuang decoction on polycystic ovary syndrome hyperandrogenism","authors":"Zhao Yiming , Zheng Dongxue , Cheng Ran , Xu Xin , Lian Fengmei , Tong Xiaolin , Liu Xinmin","doi":"10.1016/j.jtcm.2018.03.002","DOIUrl":"10.1016/j.jtcm.2018.03.002","url":null,"abstract":"<div><h3>OBJECTIVE</h3><p>To observe the efficacy and safety of different dosages of modified Zhibaidihuang decoction (MZBDD) on polycystic ovary syndrome (PCOS) hyperandrogenism (HA) patients.</p></div><div><h3>METHODS</h3><p>Ninety PCOS HA patients and 30 infertile patients due to tubal factor were selected. Ninety PCOS HA patients were randomly divided into three groups: low dosage group (LDG), medium dosage group (MDG) and high dosage group (HDG) and infertile patients were selected as normal control group (NCG). PCOS HA patients were treated with different dosage of MZBDD for 4 weeks. For HA patients, serum total testosterone (T), estrodial (E<sub>2</sub>), follicle stimulating hormone (FSH), luteinizing hormone (LH), prolatin (PRL), alanine aminotransferase (AST), aspartate amino transferase (AST) were determined before and after treatment, while acne scores (Rosenfield) were signed. Basal body temperatures (BBT) were asked to be measured every day. And for the else, T, E<sub>2</sub>, FSH, LH, PRL, AST, and AST were determined before treatment.</p></div><div><h3>RESULTS</h3><p>Totally 111 patients completed the clinical research. There were no differences among the four groups on serum T before treatment (<em>P</em> = 0.221). Serum T concentration of both MDG and HDG after treatment significantly were lower than that of before treatment (<em>P</em> = 0.039, <em>P</em> = 0.000), while there was no obvious difference in LDG (<em>P</em> = 0.829). Serum T concentration of both MDG and HDG were significantly lower than that of LDG after treatment (<em>P</em> = 0.048, <em>P</em> = 0.006). To compared with before treatment, there were no differences in Serum FSH, LH, E<sub>2</sub>, P and PRL among the three groups (LDG, MDG, HDG) (as for FSH, <em>P</em> = 0.136, <em>P</em> = 0.503, <em>P</em> = 0.062; as for LH, <em>P</em> = 0.473, <em>P</em> = 0.513, <em>P</em> = 0.096; as for E<sub>2</sub>, <em>P</em> = 0.206, <em>P</em> = 0.927, <em>P</em> = 0.076; as for PRL, <em>P</em> = 0.120, <em>P</em> = 0.903, <em>P</em> = 0.407, as for P, <em>P</em> = 0.308, <em>P</em> = 0.866, <em>P</em> = 0480). Acne scores of all the three groups were obviously lower than that of before treatment (<em>P</em> = 0.031; <em>P</em> = 0.033; <em>P</em> = 0.002). 39.5% of the patients had biphasic BBT, but there were no differences among the three groups (<em>P</em> = 0.510). There were no differences with ALT and AST between after and before treatment among the three groups (LDG, MDG, HDG) (as for ALT, <em>P</em> = 0.742, <em>P</em> = 0.383, <em>P</em> = 0.053; as for AST, <em>P</em> = 0.732, <em>P</em> = 0.519, <em>P</em> = 0.120).</p></div><div><h3>CONCLUSION</h3><p>Different dosage of MZBDD has dose-effect relationship in treating PCOS HA.</p></div>","PeriodicalId":17513,"journal":{"name":"Journal of Traditional Chinese Medicine","volume":"38 2","pages":"Pages 280-286"},"PeriodicalIF":2.6,"publicationDate":"2018-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/j.jtcm.2018.03.002","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"120815022","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2018-04-01DOI: 10.1016/j.jtcm.2018.03.007
Zhang Hewei, Ma Shuran, Tian Tian, Xu Ya, Zhao Dandan, Wang Tong, Hou Chunying, Zhang Shujing
OBJECTIVE
To investigate the inhibitory effect of Sumu (Lignum Sappan) plus Fuzi (Radix Aconiti Lateralis Praeparata) (SF) on the growth and metastasis of Lewis lung carcinoma.
METHODS
A lung carcinoma model was established by subcutaneously inoculating Lewis lung carcinoma cells into C57BL/6 mice. The mice were randomly divided into four groups of 13 mice (control, low-dose, moderate-dose, and high-dose), and gavaged once-daily for 21 consecutive days. The rates of tumor inhibition, metastasis, and metastasis inhibition were observed. The differential expressions of sP-selectin and vascular endothelial growth factor C (VEGFC) were compared between the treatment groups and the control group.
RESULTS
The tumor weights differed significantly between the treatment groups and the control group (P < 0.05). Administration of SF in the moderate-dosage and low-dose groups significantly inhibited the expression of sP-selectin and VEGFC (both P < 0.05), suggesting anti-tumor activity.
CONCLUSION
SF can inhibit the growth and metastasis of Lewis lung carcinoma.
{"title":"Inhibitory effect of Sumu (Lignum Sappan) plus Fuzi (Radix Aconiti Lateralis Praeparata) on a lung carcinoma model","authors":"Zhang Hewei, Ma Shuran, Tian Tian, Xu Ya, Zhao Dandan, Wang Tong, Hou Chunying, Zhang Shujing","doi":"10.1016/j.jtcm.2018.03.007","DOIUrl":"10.1016/j.jtcm.2018.03.007","url":null,"abstract":"<div><h3>OBJECTIVE</h3><p>To investigate the inhibitory effect of Sumu (<em>Lignum Sappan</em>) plus Fuzi (<em>Radix Aconiti Lateralis Praeparata</em>) (SF) on the growth and metastasis of Lewis lung carcinoma.</p></div><div><h3>METHODS</h3><p>A lung carcinoma model was established by subcutaneously inoculating Lewis lung carcinoma cells into C57BL/6 mice. The mice were randomly divided into four groups of 13 mice (control, low-dose, moderate-dose, and high-dose), and gavaged once-daily for 21 consecutive days. The rates of tumor inhibition, metastasis, and metastasis inhibition were observed. The differential expressions of sP-selectin and vascular endothelial growth factor C (VEGFC) were compared between the treatment groups and the control group.</p></div><div><h3>RESULTS</h3><p>The tumor weights differed significantly between the treatment groups and the control group (<em>P</em> < 0.05). Administration of SF in the moderate-dosage and low-dose groups significantly inhibited the expression of sP-selectin and VEGFC (both <em>P</em> < 0.05), suggesting anti-tumor activity.</p></div><div><h3>CONCLUSION</h3><p>SF can inhibit the growth and metastasis of Lewis lung carcinoma.</p></div>","PeriodicalId":17513,"journal":{"name":"Journal of Traditional Chinese Medicine","volume":"38 2","pages":"Pages 211-217"},"PeriodicalIF":2.6,"publicationDate":"2018-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/j.jtcm.2018.03.007","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"77804705","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2018-02-01DOI: 10.1016/j.jtcm.2018.01.005
Li Wenxiong , Zhang Kuaiqiang , Liu Zhu , Liu Li , Cheng Yan , Yin Jichao , Sun Yindi , Yang Feng
OBJECTIVE
To evaluate the effect and safety of Zuogui pill and Yougui pill, classic Yin and Yang tonic formula (CYYTF), in the treatment of osteoporosis and the underlying mechanism.
METHODS
Participants aged 55 to 75 with osteoporosis and Kidney deficiency in Traditional Chinese Medicine (TCM) will be included and randomly allocated into two groups: treatment group and control group. Participants in the treatment group were treated with Zuogui pill or Yougui pill TCM formula granule, while the control group received placebo. Primary outcomes are the lumbar spine on bone mineral density (BMD) (L1-4) and femoral BMD. Secondary outcomes include pain intensity, health-related quality of life (HRQoL), bone turnover markers and safety.
RESULTS
Totally 200 patients were enrolled from December 2014 to April 2016 from four hospitals. There were no statistically significant differences between the two groups at baseline (P > 0.05) and it was good to comparability. Statistically significant differences between the two groups were observed for the lumbar BMD (L1-4), pain VAS scores and HRQoL at six months and twelve months and femoral BMD at twelve months (P < 0.05), but no significant differences for femoral BMD and bone turnover markers at six months (P > 0.05). Moreover, significant difference was observed at different time before and after treatment in terms of lumbar spine (L1-4) BMD, femoral BMD, pain VAS scores and health-related quality of life, and there was an crossover effect between the time and groups before and after treatment. In additional, in the treatment group, 8 patients lost to follow-up and 3 patients had adverse events (AEs) and in the control group, 10 patients lost to follow-up and 2 patients had AEs. No remarkable differences were observed between the two groups with regard to AEs, lost rate and safety (P > 0.05).
CONCLUSION
Zuogui pill or Yougui pill could improve BMD, ease pain, relieve Kidney deficiency syndrome, improve the quality of life osteoporosis patients, inhibit bone conversion and regulate the coupling balance of bone formation and bone resorption, but long-term efficacy should be confirmed by a longer term follow-up and larger of samples clinical randomized controlled trials.
{"title":"Effect of Zuogui pill and Yougui pill on osteoporosis: a randomized controlled trial","authors":"Li Wenxiong , Zhang Kuaiqiang , Liu Zhu , Liu Li , Cheng Yan , Yin Jichao , Sun Yindi , Yang Feng","doi":"10.1016/j.jtcm.2018.01.005","DOIUrl":"10.1016/j.jtcm.2018.01.005","url":null,"abstract":"<div><h3>OBJECTIVE</h3><p>To evaluate the effect and safety of Zuogui pill and Yougui pill, classic <em>Yin</em> and <em>Yang</em> tonic formula (CYYTF), in the treatment of osteoporosis and the underlying mechanism.</p></div><div><h3>METHODS</h3><p>Participants aged 55 to 75 with osteoporosis and Kidney deficiency in Traditional Chinese Medicine (TCM) will be included and randomly allocated into two groups: treatment group and control group. Participants in the treatment group were treated with Zuogui pill or Yougui pill TCM formula granule, while the control group received placebo. Primary outcomes are the lumbar spine on bone mineral density (BMD) (L1-4) and femoral BMD. Secondary outcomes include pain intensity, health-related quality of life (HRQoL), bone turnover markers and safety.</p></div><div><h3>RESULTS</h3><p>Totally 200 patients were enrolled from December 2014 to April 2016 from four hospitals. There were no statistically significant differences between the two groups at baseline (<em>P</em> > 0.05) and it was good to comparability. Statistically significant differences between the two groups were observed for the lumbar BMD (L1-4), pain VAS scores and HRQoL at six months and twelve months and femoral BMD at twelve months (<em>P</em> < 0.05), but no significant differences for femoral BMD and bone turnover markers at six months (<em>P</em> > 0.05). Moreover, significant difference was observed at different time before and after treatment in terms of lumbar spine (L1-4) BMD, femoral BMD, pain VAS scores and health-related quality of life, and there was an crossover effect between the time and groups before and after treatment. In additional, in the treatment group, 8 patients lost to follow-up and 3 patients had adverse events (AEs) and in the control group, 10 patients lost to follow-up and 2 patients had AEs. No remarkable differences were observed between the two groups with regard to AEs, lost rate and safety (<em>P</em> > 0.05).</p></div><div><h3>CONCLUSION</h3><p>Zuogui pill or Yougui pill could improve BMD, ease pain, relieve Kidney deficiency syndrome, improve the quality of life osteoporosis patients, inhibit bone conversion and regulate the coupling balance of bone formation and bone resorption, but long-term efficacy should be confirmed by a longer term follow-up and larger of samples clinical randomized controlled trials.</p></div>","PeriodicalId":17513,"journal":{"name":"Journal of Traditional Chinese Medicine","volume":"38 1","pages":"Pages 33-42"},"PeriodicalIF":2.6,"publicationDate":"2018-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/j.jtcm.2018.01.005","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"73007192","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2018-02-01DOI: 10.1016/j.jtcm.2018.01.004
Xu Wenjuan , Han Qinrui , Liang Shuntian , Li Lu , Shao Meng , Yao Xueqing , Sun Xuegang
OBJECTIVE
To test the hypothesis that modified Shenlingbaizhu decoction (MSD) attenuates the formation of intestinal adenomas by regulating activation of CD4+CD25+ forkhead box P3 (FoxP3) regulatory T cells (Tregs) by downregulation of hypoxia-inducible factor 1α (HIF-1α).
METHODS
Chemical fingerprints of ginsenoside Rb1, ginsenoside Rc, paeoniflorin, and dioscin in standard extractions were used as material bases of MSD. Adenomatous polyposis coli multiple intestinal neoplasia (ApcMin/+) mice, which harbor a mutation in adenomatous polyposis coli, were used to host intestinal adenomas. Peripheral blood and spleen Tregs were analyzed by flow cytometry. Protein expression was analyzed by immunohistochemistry and Western blotting.
RESULTS
The number and size of intestinal adenomas were significantly reduced by MSD treatment. Mucosal thickening and the spleen size were also substantially decreased by MSD. The carcinogenesis process in ApcMin/+ mice resembled that of human colorectal cancer. Molecular markers of neoplasms, such as β-catenin, cyclooxygenase-2, proliferating cell nuclear antigen, and p53, were substantially ameliorated by MSD treatment. Moreover, MSD downregulated peripheral and spleen CD4 + CD25+FoxP3+Tregs and reduced in situ expression of CD4, CD25, and FoxP3 in intestinal adenomas. MSD also suppressed HIF-1α expression in the intestinal adenomas, and HIF-1α inhibition decreased expression of FoxP3 in Jurkat T cells under hypoxic conditions.
CONCLUSION
MSD is a valid prescription to control the formation of intestinal adenomas in ApcMin/+ mice. It exerts anti-cancer effects partially through suppression of HIF-1α that induced activation of CD4+CD25+FoxP3+ Tregs in vivo and in vitro.
{"title":"Modified Shenlingbaizhu decoction reduces intestinal adenoma formation in adenomatous polyposis coli multiple intestinal neoplasia mice by suppression of hypoxia-inducible factor 1α-induced CD4 + CD25+ forkhead box P3 regulatory T cells","authors":"Xu Wenjuan , Han Qinrui , Liang Shuntian , Li Lu , Shao Meng , Yao Xueqing , Sun Xuegang","doi":"10.1016/j.jtcm.2018.01.004","DOIUrl":"10.1016/j.jtcm.2018.01.004","url":null,"abstract":"<div><h3>OBJECTIVE</h3><p>To test the hypothesis that modified Shenlingbaizhu decoction (MSD) attenuates the formation of intestinal adenomas by regulating activation of CD4+CD25+ forkhead box P3 (FoxP3) regulatory T cells (Tregs) by downregulation of hypoxia-inducible factor 1α (HIF-1α).</p></div><div><h3>METHODS</h3><p>Chemical fingerprints of ginsenoside Rb1, ginsenoside Rc, paeoniflorin, and dioscin in standard extractions were used as material bases of MSD. Adenomatous polyposis coli multiple intestinal neoplasia (Apc<sup>Min/+</sup>) mice, which harbor a mutation in adenomatous polyposis coli, were used to host intestinal adenomas. Peripheral blood and spleen Tregs were analyzed by flow cytometry. Protein expression was analyzed by immunohistochemistry and Western blotting.</p></div><div><h3>RESULTS</h3><p>The number and size of intestinal adenomas were significantly reduced by MSD treatment. Mucosal thickening and the spleen size were also substantially decreased by MSD. The carcinogenesis process in Apc<sup>Min/+</sup> mice resembled that of human colorectal cancer. Molecular markers of neoplasms, such as β-catenin, cyclooxygenase-2, proliferating cell nuclear antigen, and p53, were substantially ameliorated by MSD treatment. Moreover, MSD downregulated peripheral and spleen CD4 + CD25+FoxP3+Tregs and reduced in situ expression of CD4, CD25, and FoxP3 in intestinal adenomas. MSD also suppressed HIF-1α expression in the intestinal adenomas, and HIF-1α inhibition decreased expression of FoxP3 in Jurkat T cells under hypoxic conditions.</p></div><div><h3>CONCLUSION</h3><p>MSD is a valid prescription to control the formation of intestinal adenomas in Apc<sup>Min/+</sup> mice. It exerts anti-cancer effects partially through suppression of HIF-1α that induced activation of CD4+CD25+FoxP3+ Tregs <em>in vivo</em> and <em>in vitro.</em></p></div>","PeriodicalId":17513,"journal":{"name":"Journal of Traditional Chinese Medicine","volume":"38 1","pages":"Pages 22-32"},"PeriodicalIF":2.6,"publicationDate":"2018-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/j.jtcm.2018.01.004","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"48057762","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2018-02-01DOI: 10.1016/j.jtcm.2018.02.002
Liang Chao , Wang Yuan , Xu Bin , Yu Zhi
OBJECTIVE
To observe the effect of gastric distention (GD) and acupuncture at three different acupoints on the spontaneous discharge of neurons in the medial vestibular nucleus (MVN), and to clarify the specific function of the MVN in the central integration mechanism underlying acupuncture regulation.
METHODS
GD was conducted using a balloon inserted in the stomach cavity, and acupuncture was performed separately at each of three acupoints: Zusanli (ST 36), Quchi (LI 11), and Weishu (BL 21). The effect of acupuncture and GD on the spontaneous discharge of MVN neurons was assessed using a glass microelectrode filled with a sodium acetate electrolyte solution containing 1% pontamine sky blue; the discharge signals from the neurons were amplified by the microelectrode amplifier and recorded in the Spike2 system.
RESULTS
GD and acupuncture significantly affected the spontaneous discharge of MVN neurons. Furthermore, acupuncture at Zusanli (ST 36) and Weishu (BL 21) was significantly more effective at altering the discharge of GD-responsive MVN neurons compared with GD-nonresponsive neurons.
CONCLUSION
GD and acupuncture at three different acupoints affected the electrical activity of MVN neurons. The MVN is involved in the central integration mechanism underlying acupuncture regulation of gastric functions. The effects of acupuncture on gastric function may therefore be mediated via these particular MVN neurons.
{"title":"Effect of acupuncture at three different acupoints on electrical activity of gastric distention-affected neurons in rat medial vestibular nucleus","authors":"Liang Chao , Wang Yuan , Xu Bin , Yu Zhi","doi":"10.1016/j.jtcm.2018.02.002","DOIUrl":"10.1016/j.jtcm.2018.02.002","url":null,"abstract":"<div><h3>OBJECTIVE</h3><p>To observe the effect of gastric distention (GD) and acupuncture at three different acupoints on the spontaneous discharge of neurons in the medial vestibular nucleus (MVN), and to clarify the specific function of the MVN in the central integration mechanism underlying acupuncture regulation.</p></div><div><h3>METHODS</h3><p>GD was conducted using a balloon inserted in the stomach cavity, and acupuncture was performed separately at each of three acupoints: Zusanli (ST 36), Quchi (LI 11), and Weishu (BL 21). The effect of acupuncture and GD on the spontaneous discharge of MVN neurons was assessed using a glass microelectrode filled with a sodium acetate electrolyte solution containing 1% pontamine sky blue; the discharge signals from the neurons were amplified by the microelectrode amplifier and recorded in the Spike2 system.</p></div><div><h3>RESULTS</h3><p>GD and acupuncture significantly affected the spontaneous discharge of MVN neurons. Furthermore, acupuncture at Zusanli (ST 36) and Weishu (BL 21) was significantly more effective at altering the discharge of GD-responsive MVN neurons compared with GD-nonresponsive neurons.</p></div><div><h3>CONCLUSION</h3><p>GD and acupuncture at three different acupoints affected the electrical activity of MVN neurons. The MVN is involved in the central integration mechanism underlying acupuncture regulation of gastric functions. The effects of acupuncture on gastric function may therefore be mediated <em>via</em> these particular MVN neurons.</p></div>","PeriodicalId":17513,"journal":{"name":"Journal of Traditional Chinese Medicine","volume":"38 1","pages":"Pages 125-131"},"PeriodicalIF":2.6,"publicationDate":"2018-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/j.jtcm.2018.02.002","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"44336013","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2018-02-01DOI: 10.1016/j.jtcm.2018.01.006
Han Li , Liu Changxi , Liu Ping , Hu Hai , Yang Jia , Cai Hong , Lim Minyee , Zhu Maoxiang , Pan Xiujie , Huang Jian , Cui Yingxue , Lao Lixing , Zhao Baixiao
OBJECTIVE
To assess toxicities of the air in Chinese medicine clinics polluted by moxa-burning smoke due to moxibustion-derived burning products (MBP).
METHODS
Both acute and chronic toxicity studies were conducted. For the acute toxicity study, five groups of Wistar rats (n = 16/group, male: female = 1:1) were exposed to five different concentrations (95%, 90%, 85%, 80% and 75%, respectively) of MBP for 2 h. For the chronic toxicity study, another three groups of male rats (n = 21/group) were exposed to MBP in three concentrations (10%, 40% and 70%, respectively) and one control group exposed to clean air 20 min/d for 144 d. Routine examinations were performed and analyzed by analysis of variance and dose-response relationship.
RESULTS
In the acute toxicity study, the number of dead rats in the 95%, 90%, 85%, 80% and 75% groups were 16, 13, 7, 6 and 3, respectively, with LD50 of 86.274% after or during the 2 h exposure. In the chronic toxicity study, MBP exposure induced a decline in activity of the rats. Rats in the 10% group showed no signs of toxicity, while those in the 40% MBP group showed toxicity effects on the body weights (P < 0.05) and lung. Rats in the 70% MBP group also presented with reversible damage in the blood coagulation system (P < 0.05).
CONCLUSION
Exposure to 10% MBP, which is equivalent to 27.45 mg/m3, was under the critical threshold for male rats’ safety. Exposure to MBP above that limit induced lung damage. MBP in clinics need to be reduced to a safe level with enhanced ventilation.
{"title":"Repeated exposure to moxa-burning smoke: its acute and chronic toxicities in rats","authors":"Han Li , Liu Changxi , Liu Ping , Hu Hai , Yang Jia , Cai Hong , Lim Minyee , Zhu Maoxiang , Pan Xiujie , Huang Jian , Cui Yingxue , Lao Lixing , Zhao Baixiao","doi":"10.1016/j.jtcm.2018.01.006","DOIUrl":"10.1016/j.jtcm.2018.01.006","url":null,"abstract":"<div><h3>OBJECTIVE</h3><p>To assess toxicities of the air in Chinese medicine clinics polluted by moxa-burning smoke due to moxibustion-derived burning products (MBP).</p></div><div><h3>METHODS</h3><p>Both acute and chronic toxicity studies were conducted. For the acute toxicity study, five groups of Wistar rats (<em>n</em> = 16/group, male: female = 1:1) were exposed to five different concentrations (95%, 90%, 85%, 80% and 75%, respectively) of MBP for 2 h. For the chronic toxicity study, another three groups of male rats (<em>n</em> = 21/group) were exposed to MBP in three concentrations (10%, 40% and 70%, respectively) and one control group exposed to clean air 20 min/d for 144 d. Routine examinations were performed and analyzed by analysis of variance and dose-response relationship.</p></div><div><h3>RESULTS</h3><p>In the acute toxicity study, the number of dead rats in the 95%, 90%, 85%, 80% and 75% groups were 16, 13, 7, 6 and 3, respectively, with LD50 of 86.274% after or during the 2 h exposure. In the chronic toxicity study, MBP exposure induced a decline in activity of the rats. Rats in the 10% group showed no signs of toxicity, while those in the 40% MBP group showed toxicity effects on the body weights (<em>P</em> < 0.05) and lung. Rats in the 70% MBP group also presented with reversible damage in the blood coagulation system (<em>P</em> < 0.05).</p></div><div><h3>CONCLUSION</h3><p>Exposure to 10% MBP, which is equivalent to 27.45 mg/m<sup>3</sup>, was under the critical threshold for male rats’ safety. Exposure to MBP above that limit induced lung damage. MBP in clinics need to be reduced to a safe level with enhanced ventilation.</p></div>","PeriodicalId":17513,"journal":{"name":"Journal of Traditional Chinese Medicine","volume":"38 1","pages":"Pages 67-75"},"PeriodicalIF":2.6,"publicationDate":"2018-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/j.jtcm.2018.01.006","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"48142436","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2018-02-01DOI: 10.1016/j.jtcm.2018.02.006
Zhang Jing, Li Liangyong, Chen Huaizhen, Yang Wenming
OBJECTIVE
To investigate the effect and safety of Gandouling plus low-dose D-penicillamine for treating patients with Wilson's disease (WD) who have neurological symptoms.
METHODS
WD patients with neurological symptoms were divided into two groups: a treatment group (n = 53) and a control group (n = 50). The treatment group received anti-copper therapy with a combination of Gandouling and low-dose D-penicillamine (10 mg/kg), whereas the control group was with conventional dose D-penicillamine (20 mg/kg) monotherapy. The clinical efficacies, adverse reactions, and results of the various hematological and biochemical investigations were recorded and analyzed statistically.
RESULTS
Overall, 98.11% of the WD patients treated with the combined therapy experienced alleviation of their neurological condition (paralleled by a significantly improved Global Assessment Scale score or remained stable). Their white blood cell and platelet counts stabilized, and their liver function was improved or remained stable. The combined therapy also obviously promoted improved 24-h urinary copper excretion. Only 15.09% of the WD patients with the combined therapy experienced adverse reactions, including neurological deterioration in one case (1.89%) and hepatic worsening in one case (1.89%), which was less frequent than that in the control group given conventional-dose D-penicillamine monotherapy.
CONCLUSION
Treating WD patients with neurological symptoms using Gandouling plus low-dose D-penicillamine is effective and safe.
{"title":"Clinical efficacy and safety of Gandouling plus low-dose D-penicillamine for treatment of Wilson's disease with neurological symptoms","authors":"Zhang Jing, Li Liangyong, Chen Huaizhen, Yang Wenming","doi":"10.1016/j.jtcm.2018.02.006","DOIUrl":"10.1016/j.jtcm.2018.02.006","url":null,"abstract":"<div><h3>OBJECTIVE</h3><p>To investigate the effect and safety of Gandouling plus low-dose D-penicillamine for treating patients with Wilson's disease (WD) who have neurological symptoms.</p></div><div><h3>METHODS</h3><p>WD patients with neurological symptoms were divided into two groups: a treatment group (<em>n</em> = 53) and a control group (<em>n</em> = 50). The treatment group received anti-copper therapy with a combination of Gandouling and low-dose D-penicillamine (10 mg/kg), whereas the control group was with conventional dose D-penicillamine (20 mg/kg) monotherapy. The clinical efficacies, adverse reactions, and results of the various hematological and biochemical investigations were recorded and analyzed statistically.</p></div><div><h3>RESULTS</h3><p>Overall, 98.11% of the WD patients treated with the combined therapy experienced alleviation of their neurological condition (paralleled by a significantly improved Global Assessment Scale score or remained stable). Their white blood cell and platelet counts stabilized, and their liver function was improved or remained stable. The combined therapy also obviously promoted improved 24-h urinary copper excretion. Only 15.09% of the WD patients with the combined therapy experienced adverse reactions, including neurological deterioration in one case (1.89%) and hepatic worsening in one case (1.89%), which was less frequent than that in the control group given conventional-dose D-penicillamine monotherapy.</p></div><div><h3>CONCLUSION</h3><p>Treating WD patients with neurological symptoms using Gandouling plus low-dose D-penicillamine is effective and safe.</p></div>","PeriodicalId":17513,"journal":{"name":"Journal of Traditional Chinese Medicine","volume":"38 1","pages":"Pages 89-94"},"PeriodicalIF":2.6,"publicationDate":"2018-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/j.jtcm.2018.02.006","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"44367020","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2018-02-01DOI: 10.1016/j.jtcm.2018.02.003
Li Jing , Zheng Chunquan , Lin Hai , Yang Chen , Gu Siyuan , Wang Yi , Duan Honggang
OBJECTIVE
To investigate the effectiveness and safety of Zhu-yuan decoction (ZYD) in patients after functional endoscopic sinus surgery (FESS).
METHODS
A total of 85 patients were randomized into two groups: 44 were treated with intranasal corticosteroids (INC), and 41 were given Chinese herbal medicine (CHM). Patients with chronic rhinosinusitis (CRS) who underwent FESS were prospectively enrolled in the study. Before surgery, they were evaluated by visual analog scale (VAS), nasal endoscopy, computed tomography (CT), and routine blood test. After surgery, they were randomized to take ZYD or INC for 12 weeks and revaluated by VAS; nasal endoscopy at 4, 8, and 12 weeks; and CT at 12 weeks after surgery.
RESULTS
In the both groups, VAS and endoscopy scores decreased significantly at 4, 8, and 12 weeks, and CT scores after treatment declined at 12 weeks compared with baseline scores. No significant differences were observed with regard to postoperative VAS, endoscopy, or CT scores between groups. ZYD, combined with surgery, can reduce VAS, nasal endoscopy, and CT scores and has the same efficacy and safety profile as INC in post-FESS management. No fatalities or major adverse events occurred in either group.
CONCLUSION
Our findings suggest that ZYD has similar effects and safety profiles in patients after FESS compared with INC.
{"title":"Effect of Zhu-yuan decoction in patients with chronic rhinosinusitis after functional endoscopic sinus surgery","authors":"Li Jing , Zheng Chunquan , Lin Hai , Yang Chen , Gu Siyuan , Wang Yi , Duan Honggang","doi":"10.1016/j.jtcm.2018.02.003","DOIUrl":"10.1016/j.jtcm.2018.02.003","url":null,"abstract":"<div><h3>OBJECTIVE</h3><p>To investigate the effectiveness and safety of Zhu-yuan decoction (ZYD) in patients after functional endoscopic sinus surgery (FESS).</p></div><div><h3>METHODS</h3><p>A total of 85 patients were randomized into two groups: 44 were treated with intranasal corticosteroids (INC), and 41 were given Chinese herbal medicine (CHM). Patients with chronic rhinosinusitis (CRS) who underwent FESS were prospectively enrolled in the study. Before surgery, they were evaluated by visual analog scale (VAS), nasal endoscopy, computed tomography (CT), and routine blood test. After surgery, they were randomized to take ZYD or INC for 12 weeks and revaluated by VAS; nasal endoscopy at 4, 8, and 12 weeks; and CT at 12 weeks after surgery.</p></div><div><h3>RESULTS</h3><p>In the both groups, VAS and endoscopy scores decreased significantly at 4, 8, and 12 weeks, and CT scores after treatment declined at 12 weeks compared with baseline scores. No significant differences were observed with regard to postoperative VAS, endoscopy, or CT scores between groups. ZYD, combined with surgery, can reduce VAS, nasal endoscopy, and CT scores and has the same efficacy and safety profile as INC in post-FESS management. No fatalities or major adverse events occurred in either group.</p></div><div><h3>CONCLUSION</h3><p>Our findings suggest that ZYD has similar effects and safety profiles in patients after FESS compared with INC.</p></div>","PeriodicalId":17513,"journal":{"name":"Journal of Traditional Chinese Medicine","volume":"38 1","pages":"Pages 83-88"},"PeriodicalIF":2.6,"publicationDate":"2018-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/j.jtcm.2018.02.003","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"49507084","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2018-02-01DOI: 10.1016/j.jtcm.2018.01.007
Yang Jiamin , Wu Jihui , Shen Xiaoyu , Zhang Mei , Liu Zhenzhen , Qi Dandan , Zhou Jin , Cui Xiao , Zhang Lufen , Li Yuhang
OBJECTIVE
To evaluate the efficacy of moxibustion, through stimulating acupoints of Danzhong (CV 17) and Ganshu (BL 18) in rats with hyperplasia of mammary gland (HMG) which induced by estrogen and progestogen.
METHODS
Thirty female Sprague-Dawley rats were randomly divided into saline control group, HMG model group, and HMG moxibustion group with 10 in each group. Saline control was the group injected by saline. HMG model were created by injection of estrogen and progestogen. Moxibustion group was also injected of estrogen and progestogen with moxibustion at the same time. The Changes of nipple diameter and height were measured. The rats’ skin temperature was recorded by an infrared thermal camera at the nipples, mammary areas, Danzhong (CV 17) and Ganshu (BL 18). Pathological changes of mammary gland in rats were also observed under light microscope.
RESULTS
The diameter and height of the nipples in model group were prominently bigger and higher than that in control group (P < 0.01). The diameter and height in moxibustion group were prominently smaller and lower than that in model group (P < 0.01), and there was no significant difference between moxibustion group and control group. Compared with control group, skin temperature of the nipples, mammary area, and acupoints Danzhong (CV 17) and Ganshu (BL 18) decreased prominently in model group (P < 0.01-0.05). Compared with model group, skin temperature of that in moxibustion group increased prominently (P < 0.05).
CONCLUSION
Treatment with moxibustion can effectively decrease the HMG rats’ nipple diameter and height, and increase the skin temperature in HMG model rats at the nipples, mammary areas, Danzhong (CV 17) and Ganshu (BL 18). This study convinces the therapeutic effect of moxibustion on mammary gland hyperplasia.
{"title":"Efficacy of moxibustion by stimulating acupoints of Danzhong (CV 17) and Ganshu (BL 18) on hyperplasia of mammary gland in rats","authors":"Yang Jiamin , Wu Jihui , Shen Xiaoyu , Zhang Mei , Liu Zhenzhen , Qi Dandan , Zhou Jin , Cui Xiao , Zhang Lufen , Li Yuhang","doi":"10.1016/j.jtcm.2018.01.007","DOIUrl":"10.1016/j.jtcm.2018.01.007","url":null,"abstract":"<div><h3>OBJECTIVE</h3><p>To evaluate the efficacy of moxibustion, through stimulating acupoints of Danzhong (CV 17) and Ganshu (BL 18) in rats with hyperplasia of mammary gland (HMG) which induced by estrogen and progestogen.</p></div><div><h3>METHODS</h3><p>Thirty female Sprague-Dawley rats were randomly divided into saline control group, HMG model group, and HMG moxibustion group with 10 in each group. Saline control was the group injected by saline. HMG model were created by injection of estrogen and progestogen. Moxibustion group was also injected of estrogen and progestogen with moxibustion at the same time. The Changes of nipple diameter and height were measured. The rats’ skin temperature was recorded by an infrared thermal camera at the nipples, mammary areas, Danzhong (CV 17) and Ganshu (BL 18). Pathological changes of mammary gland in rats were also observed under light microscope.</p></div><div><h3>RESULTS</h3><p>The diameter and height of the nipples in model group were prominently bigger and higher than that in control group (<em>P</em> < 0.01). The diameter and height in moxibustion group were prominently smaller and lower than that in model group (<em>P</em> < 0.01), and there was no significant difference between moxibustion group and control group. Compared with control group, skin temperature of the nipples, mammary area, and acupoints Danzhong (CV 17) and Ganshu (BL 18) decreased prominently in model group (<em>P</em> < 0.01-0.05). Compared with model group, skin temperature of that in moxibustion group increased prominently (<em>P</em> < 0.05).</p></div><div><h3>CONCLUSION</h3><p>Treatment with moxibustion can effectively decrease the HMG rats’ nipple diameter and height, and increase the skin temperature in HMG model rats at the nipples, mammary areas, Danzhong (CV 17) and Ganshu (BL 18). This study convinces the therapeutic effect of moxibustion on mammary gland hyperplasia.</p></div>","PeriodicalId":17513,"journal":{"name":"Journal of Traditional Chinese Medicine","volume":"38 1","pages":"Pages 76-82"},"PeriodicalIF":2.6,"publicationDate":"2018-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/j.jtcm.2018.01.007","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"45044037","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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