Pub Date : 2017-02-05DOI: 10.4172/2157-7552.100I101
Saeed Ahmadi Majd, M. R. Khorasgani, Firouzeh Khosravi
Nowadays, Diabetes is one of the most common diseases in the world. These diseases and disorders of the problems for the patient and the health system create. One of the long term complications of diabetes is neuropathy, or nerve problems which are involved in diabetic foot ulcers and prevalence of this disorder in patients between 12 and 50 percent mentioned. Diabetes mellitus is a group of metabolic diseases characterized by hyperglycemia resulting from defects in insulin secretion, insulin action, or both. Regarding the absence of sufficient efficacy of the available methods in diabetic wound healing, the use of alternative or complementary compounds has been considered (Figure 1 and 2).
{"title":"Novel Combinations for Wound Healing: Chitosan/PVA Nano Fiber; Royal Jelly,Probiotic","authors":"Saeed Ahmadi Majd, M. R. Khorasgani, Firouzeh Khosravi","doi":"10.4172/2157-7552.100I101","DOIUrl":"https://doi.org/10.4172/2157-7552.100I101","url":null,"abstract":"Nowadays, Diabetes is one of the most common diseases in the world. These diseases and disorders of the problems for the patient and the health system create. One of the long term complications of diabetes is neuropathy, or nerve problems which are involved in diabetic foot ulcers and prevalence of this disorder in patients between 12 and 50 percent mentioned. Diabetes mellitus is a group of metabolic diseases characterized by hyperglycemia resulting from defects in insulin secretion, insulin action, or both. Regarding the absence of sufficient efficacy of the available methods in diabetic wound healing, the use of alternative or complementary compounds has been considered (Figure 1 and 2).","PeriodicalId":17539,"journal":{"name":"Journal of Tissue Science and Engineering","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2017-02-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"82589754","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2017-02-03DOI: 10.4172/2157-7552.1000194
Xiao-Feng Pang, Li Gun
The influences of electromagnetic field(EMF) of high voltage transmission lines(HVTL) with 50 Hz on the features of blood in the animals, including the biochemical indicators, routine index, refractive index and infrared absorption of the serum and adtevak as well as the features of hemoglobin molecules in it, are researched and measured extensively by using the biological- chemical methods, LQ-300 K automatic biochemistry analyzer, 670Nicolet FTIR spectrometers and Abbe refractometer, respectively. The results obtained showed the variations for the alanine aminotransferase (ALT), total protein (TP), albumin (ALB), total bilirubin (TB), direct bilirubin (DB), indirect bilirubin (IB), globulin (CLO), aspartate aminotransferase (AST), ratio of ALB with CLO (A/C), urea nitrogen (BUN), direct bilirubin (DB), globulin (GLO) as well as refractive index in the blood under the influences of the EMF, but their variations are differences, in which the variation of ALT is most significant (P<0.05), the increase of number of white blood cells is also significant (P<0.01). Thus we can affirm that the EMF of HVTL can vary the properties of the blood in rats. At the same time, we further elucidated the reasons or mechanisms of these change, for which we can say totally that they are due to the variations of the states and properties of the protein molecules, such as the hemoglobin, in blood. This conclusion can be verified by the experimental results in the infrared absorptions of the serum, adtevak and hemoglobin molecules, respectively. Therefore, our investigations manifested clearly that the EMF irradiated by HVTL can influence and vary the properties of the blood in rats; it has an obvious biological effect.
{"title":"The Influences of Electromagnetic Field Irradiated by High Voltage Transmission Lines with 50 Hz on the Features of Blood in Animals","authors":"Xiao-Feng Pang, Li Gun","doi":"10.4172/2157-7552.1000194","DOIUrl":"https://doi.org/10.4172/2157-7552.1000194","url":null,"abstract":"The influences of electromagnetic field(EMF) of high voltage transmission lines(HVTL) with 50 Hz on the features of blood in the animals, including the biochemical indicators, routine index, refractive index and infrared absorption of the serum and adtevak as well as the features of hemoglobin molecules in it, are researched and measured extensively by using the biological- chemical methods, LQ-300 K automatic biochemistry analyzer, 670Nicolet FTIR spectrometers and Abbe refractometer, respectively. The results obtained showed the variations for the alanine aminotransferase (ALT), total protein (TP), albumin (ALB), total bilirubin (TB), direct bilirubin (DB), indirect bilirubin (IB), globulin (CLO), aspartate aminotransferase (AST), ratio of ALB with CLO (A/C), urea nitrogen (BUN), direct bilirubin (DB), globulin (GLO) as well as refractive index in the blood under the influences of the EMF, but their variations are differences, in which the variation of ALT is most significant (P<0.05), the increase of number of white blood cells is also significant (P<0.01). Thus we can affirm that the EMF of HVTL can vary the properties of the blood in rats. At the same time, we further elucidated the reasons or mechanisms of these change, for which we can say totally that they are due to the variations of the states and properties of the protein molecules, such as the hemoglobin, in blood. This conclusion can be verified by the experimental results in the infrared absorptions of the serum, adtevak and hemoglobin molecules, respectively. Therefore, our investigations manifested clearly that the EMF irradiated by HVTL can influence and vary the properties of the blood in rats; it has an obvious biological effect.","PeriodicalId":17539,"journal":{"name":"Journal of Tissue Science and Engineering","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2017-02-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"84205788","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2017-01-30DOI: 10.4172/2157-7552.1000190
Rahul Upadhya
microRNA (miRNA) is non-coding RNA sequences that modify DNA, typically through cleavages. They can have an effect on cell cycle progression, namely through inhibiting regulators for tumorigenesis control. Because of this, specific miRNA sequences can be monitored for cancer detection purposes. This detection is conducted through a variety of methods, such as in tissue, blood plasma, serum, ductal lavage fluid and nipple aspirate fluid. Recent research has been done to compare miRNA detection accuracy between these methods, especially with tissue analyses since this tends to be more invasive. Imaging methods have been utilized for cancer detection, but they are less effective in staging cancers, which is necessary for clinicians to provide individualized treatments. In several cases, detection accuracy has been compared to the accuracy from biopsy methods, which is a common practice but also invasive. In the future, non-invasive techniques of detection and personalized treatments will be the emphasis of research in cancer detection.
{"title":"Tissue microRNAs as Cancer Tissue Biomarkers","authors":"Rahul Upadhya","doi":"10.4172/2157-7552.1000190","DOIUrl":"https://doi.org/10.4172/2157-7552.1000190","url":null,"abstract":"microRNA (miRNA) is non-coding RNA sequences that modify DNA, typically through cleavages. They can have an effect on cell cycle progression, namely through inhibiting regulators for tumorigenesis control. Because of this, specific miRNA sequences can be monitored for cancer detection purposes. This detection is conducted through a variety of methods, such as in tissue, blood plasma, serum, ductal lavage fluid and nipple aspirate fluid. Recent research has been done to compare miRNA detection accuracy between these methods, especially with tissue analyses since this tends to be more invasive. Imaging methods have been utilized for cancer detection, but they are less effective in staging cancers, which is necessary for clinicians to provide individualized treatments. In several cases, detection accuracy has been compared to the accuracy from biopsy methods, which is a common practice but also invasive. In the future, non-invasive techniques of detection and personalized treatments will be the emphasis of research in cancer detection.","PeriodicalId":17539,"journal":{"name":"Journal of Tissue Science and Engineering","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2017-01-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"88686344","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2017-01-01DOI: 10.4172/2157-7552.1000206
S. Shrivastava, S. Naik, Bhushan Patil, Pankaj Kharabe, Anurag Gupta, Swapnil Joshi
With the advent of Regenerative medicine products in medicine, newer solutions are getting evolved for complex conditions. The Cellular therapies offer a new ray of hope by inducing regeneration of tissue at the site of damage. The cellular therapy led by platelet rich plasma (PRP) is one such key regenerative product. But there is a huge variability in its usage, particularly for wounds. The standardisation for clinical usage is not yet evolved, hence limiting the clinical application of the same. The authors have developed a standard protocol “STARS” therapy, which utilises PRP as biotechnological tool, re-engineering the damaged tissue and gradually assisting the regeneration. In this study we report 8 cases of wounds / ulcers over the sole being treated by it. The results are excellent and offer a milestone solution to non-healing wounds/ ulcers over soles. These wounds are otherwise, very difficult to treat particularly as they are associated with high risks factors such as infection, Diabetes and old age. They are under constant pressure due to weight bearing and give way easily and do not heal. The bioenginreeing of the sole tissues through PRP led STARS intervention is safe, effective and easily reproducible.
{"title":"Bio Technological Intervention with Platelet Rich Plasma for Assisted Regeneration of Sole","authors":"S. Shrivastava, S. Naik, Bhushan Patil, Pankaj Kharabe, Anurag Gupta, Swapnil Joshi","doi":"10.4172/2157-7552.1000206","DOIUrl":"https://doi.org/10.4172/2157-7552.1000206","url":null,"abstract":"With the advent of Regenerative medicine products in medicine, newer solutions are getting evolved for complex conditions. The Cellular therapies offer a new ray of hope by inducing regeneration of tissue at the site of damage. The cellular therapy led by platelet rich plasma (PRP) is one such key regenerative product. But there is a huge variability in its usage, particularly for wounds. The standardisation for clinical usage is not yet evolved, hence limiting the clinical application of the same. The authors have developed a standard protocol “STARS” therapy, which utilises PRP as biotechnological tool, re-engineering the damaged tissue and gradually assisting the regeneration. In this study we report 8 cases of wounds / ulcers over the sole being treated by it. The results are excellent and offer a milestone solution to non-healing wounds/ ulcers over soles. These wounds are otherwise, very difficult to treat particularly as they are associated with high risks factors such as infection, Diabetes and old age. They are under constant pressure due to weight bearing and give way easily and do not heal. The bioenginreeing of the sole tissues through PRP led STARS intervention is safe, effective and easily reproducible.","PeriodicalId":17539,"journal":{"name":"Journal of Tissue Science and Engineering","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2017-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"90559672","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2017-01-01DOI: 10.4172/2157-7552.1000212
F. Alimirzaei, E. Vasheghani-Farahani, Ali Ghiaseddin, M. Soleimani, Pouri, Zeinab Najafi-Gharavi
Recently in situ injectable hydrogels have received considerable attention to regenerate failure heart tissue after myocardial infarction (MI). But two criteria remain crucial: first, defining their chemical composition and structural parameters because they must be fabricated to withstand physiological condition while having high cell retention; second, providing the hydrogel with short gelation time to protect the cells from washout and extrusion in the injection site. In this study, two different pH-sensitive hydrogels with instant gelation in the presence of aqueous acetic acid (WH sample), and DMEM medium along with acetic acid (MH sample) were prepared. The morphology and structural and mechanical properties of the hydrogels were evaluated by SEM, FTIR and compressive strength test, respectively. Also, the equilibrium swelling ratio of each hydrogel and their degradation rate as a desirable property were also determined. MTT test confirmed that both hydrogels were biocompatible and non-toxic for human Bone Marrow Mesenchymal Stem Cells (hBMSCs) within 14 days as well as 31 and 36% cell growth during this time for WH and MH samples, respectively. The viability investigation of human Adipose Mesenchymal Stem Cells (hADSCs), tested by live/dead assay with Acridine Orange and Ethidium bromide staining, showed high cell survival and cell density increase during 1, 7, 14, 21 days. The overall results demonstrated that both in situ forming hydrogels are promising candidates for regeneration of the infarcted myocardium.
{"title":"pH-Sensitive Chitosan Hydrogel with Instant Gelation for Myocardial Regeneration","authors":"F. Alimirzaei, E. Vasheghani-Farahani, Ali Ghiaseddin, M. Soleimani, Pouri, Zeinab Najafi-Gharavi","doi":"10.4172/2157-7552.1000212","DOIUrl":"https://doi.org/10.4172/2157-7552.1000212","url":null,"abstract":"Recently in situ injectable hydrogels have received considerable attention to regenerate failure heart tissue after myocardial infarction (MI). But two criteria remain crucial: first, defining their chemical composition and structural parameters because they must be fabricated to withstand physiological condition while having high cell retention; second, providing the hydrogel with short gelation time to protect the cells from washout and extrusion in the injection site. In this study, two different pH-sensitive hydrogels with instant gelation in the presence of aqueous acetic acid (WH sample), and DMEM medium along with acetic acid (MH sample) were prepared. The morphology and structural and mechanical properties of the hydrogels were evaluated by SEM, FTIR and compressive strength test, respectively. Also, the equilibrium swelling ratio of each hydrogel and their degradation rate as a desirable property were also determined. MTT test confirmed that both hydrogels were biocompatible and non-toxic for human Bone Marrow Mesenchymal Stem Cells (hBMSCs) within 14 days as well as 31 and 36% cell growth during this time for WH and MH samples, respectively. The viability investigation of human Adipose Mesenchymal Stem Cells (hADSCs), tested by live/dead assay with Acridine Orange and Ethidium bromide staining, showed high cell survival and cell density increase during 1, 7, 14, 21 days. The overall results demonstrated that both in situ forming hydrogels are promising candidates for regeneration of the infarcted myocardium.","PeriodicalId":17539,"journal":{"name":"Journal of Tissue Science and Engineering","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2017-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"74919816","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2017-01-01DOI: 10.4172/2157-7552.1000205
N. Khan
Employing cationic lipids and polymers, inorganic nanoparticles, etc. as DNA carriers for gene delivery systems are gaining interest with the passage of time such chemical vectors form condensed complexes or aggregates with negatively charged DNA to not only protect the DNA from nucleases but also facilitate its intracellular uptake and site specific delivery.
{"title":"Gene Delivery System: Non-Viral Mediated Chemical Approaches","authors":"N. Khan","doi":"10.4172/2157-7552.1000205","DOIUrl":"https://doi.org/10.4172/2157-7552.1000205","url":null,"abstract":"Employing cationic lipids and polymers, inorganic nanoparticles, etc. as DNA carriers for gene delivery systems are gaining interest with the passage of time such chemical vectors form condensed complexes or aggregates with negatively charged DNA to not only protect the DNA from nucleases but also facilitate its intracellular uptake and site specific delivery.","PeriodicalId":17539,"journal":{"name":"Journal of Tissue Science and Engineering","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2017-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"78276111","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2016-12-26DOI: 10.4172/2157-7552.1000189
Ruhma Mahmood, Mahmood S Choudhery, Shaheen N. Khan, S. Riazuddin
Considering importance of stem cell based therapies for wound healing, we have previously established protocols for successful differentiation of stem cells (isolated from placenta) into skin-like cells (keratinocytes and fibroblasts). In the current study we aim to evaluate the effect of pre-differentiated skin-like-cells on wound healing potential using an in vitro burn injury model. The amniotic epithelial cells (AECs) and umbilical cord mesenchymal stem cells (UCMSCs) obtained from amniotic membrane and umbilical cord tissue; respectively, were differentiated into skin-like cells (keratinocyte and fibroblasts respectively). In order to make an in vitro burn injury model, keratinocytes and fibroblasts were isolated from rats and insulted with high temperature (up to 50oC). The optimal temperature for burn injury models was determined using viability assay, cytotoxicity level, proliferation, and expression of pro-apoptotic. In order to assess effectiveness of pre-differentiated skin like cells, the keratinocyte- and fibroblasts-like cells were co-cultured with injured keratinocytes and injured fibroblasts, respectively. Results indicated that optimal temperature for the induction of heat injury for both keratinocytes and fibroblasts was 50oC. At this temperature both types of cells (keratinocytes and fibroblasts) showed modified morphology, drastic cellular injury, least viability, higher expression of pro-apoptotic markers and lower expression of proliferation genes. Co-culturing of pre-differentiated cells leads to an increase in viability and proliferation concurrent with decreased apoptosis. We have established successfully in vitro model of burn injury that might be helpful to evaluate the wound healing potential of cells. Further, predifferentiated skin-like cells are a potential source for the treatment of severe burn injuries.
{"title":"Pre-Differentiated Skin-Like Cells can Alleviate In Vitro Burn Injury","authors":"Ruhma Mahmood, Mahmood S Choudhery, Shaheen N. Khan, S. Riazuddin","doi":"10.4172/2157-7552.1000189","DOIUrl":"https://doi.org/10.4172/2157-7552.1000189","url":null,"abstract":"Considering importance of stem cell based therapies for wound healing, we have previously established protocols for successful differentiation of stem cells (isolated from placenta) into skin-like cells (keratinocytes and fibroblasts). In the current study we aim to evaluate the effect of pre-differentiated skin-like-cells on wound healing potential using an in vitro burn injury model. The amniotic epithelial cells (AECs) and umbilical cord mesenchymal stem cells (UCMSCs) obtained from amniotic membrane and umbilical cord tissue; respectively, were differentiated into skin-like cells (keratinocyte and fibroblasts respectively). In order to make an in vitro burn injury model, keratinocytes and fibroblasts were isolated from rats and insulted with high temperature (up to 50oC). The optimal temperature for burn injury models was determined using viability assay, cytotoxicity level, proliferation, and expression of pro-apoptotic. In order to assess effectiveness of pre-differentiated skin like cells, the keratinocyte- and fibroblasts-like cells were co-cultured with injured keratinocytes and injured fibroblasts, respectively. Results indicated that optimal temperature for the induction of heat injury for both keratinocytes and fibroblasts was 50oC. At this temperature both types of cells (keratinocytes and fibroblasts) showed modified morphology, drastic cellular injury, least viability, higher expression of pro-apoptotic markers and lower expression of proliferation genes. Co-culturing of pre-differentiated cells leads to an increase in viability and proliferation concurrent with decreased apoptosis. We have established successfully in vitro model of burn injury that might be helpful to evaluate the wound healing potential of cells. Further, predifferentiated skin-like cells are a potential source for the treatment of severe burn injuries.","PeriodicalId":17539,"journal":{"name":"Journal of Tissue Science and Engineering","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2016-12-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"74599368","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2016-12-16DOI: 10.4172/2157-7552.1000188
S. Cook, G. Sandusky
A post mortem examination was performed on a 45 year old white male who died suddenly during a competitive athletic swimming event. There were few clinical events seen in the year before his death. Clinical workup was negative and lung scan showed a small coin lesion was attributed to focal bacterial/fungal infection. Antibiotic treatment appeared to clear this condition on another follow up scan and he was sent home with an inhaler. On gross post mortem examination the lungs revealed diffuse pulmonary congestion, focal hemorrhage and edema. Small focal white areas of calcified lesions were also seen. In addition, an enlarged heart with left ventricular hypertrophy and thickened interventricular septum were seen. There was moderate atherosclerosis midway down the left anterior descending branch of the coronary artery observed. Histologic examination of lung tissue proved confirmatory for diffuse pulmonary ossification. Heart microscopic examination was consistent with cardiomyocyte hypertrophy. In conclusion, the diffuse pulmonary ossification was probably related to the sudden death with concomitant myocardial hypertrophy.
{"title":"A Post Mortem Case Study: Diffuse Pulmonary Ossification and Sudden Death","authors":"S. Cook, G. Sandusky","doi":"10.4172/2157-7552.1000188","DOIUrl":"https://doi.org/10.4172/2157-7552.1000188","url":null,"abstract":"A post mortem examination was performed on a 45 year old white male who died suddenly during a competitive athletic swimming event. There were few clinical events seen in the year before his death. Clinical workup was negative and lung scan showed a small coin lesion was attributed to focal bacterial/fungal infection. Antibiotic treatment appeared to clear this condition on another follow up scan and he was sent home with an inhaler. On gross post mortem examination the lungs revealed diffuse pulmonary congestion, focal hemorrhage and edema. Small focal white areas of calcified lesions were also seen. In addition, an enlarged heart with left ventricular hypertrophy and thickened interventricular septum were seen. There was moderate atherosclerosis midway down the left anterior descending branch of the coronary artery observed. Histologic examination of lung tissue proved confirmatory for diffuse pulmonary ossification. Heart microscopic examination was consistent with cardiomyocyte hypertrophy. In conclusion, the diffuse pulmonary ossification was probably related to the sudden death with concomitant myocardial hypertrophy.","PeriodicalId":17539,"journal":{"name":"Journal of Tissue Science and Engineering","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2016-12-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"87416247","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2016-12-10DOI: 10.4172/2157-7552.1000187
Deb, I. Maria, C. Bernal, N. Francois
In the present work, chitosan/glycerol blends were used to prepare biodegradable films with potential application in dermal treatment. While the presence of glycerol gives the hydrating effect, the chitosan characteristics contribute to dermal regeneration. Swelling degree measurements, water vapor permeability determinations, mechanical tests, scanning electronic microscopy and Fourier transform infrared spectroscopy analysis; biodegradability studies, thermogravimetric and differential scanning calorimetry analysis were used to analyze the influence of the incorporation of significant amounts of glycerol in film properties. The obtained results suggested that the glycerol addition provide the films with the required properties for the intended application related to dermal treatment. Increasing the glycerol concentration, a cluster effect produced by the glycerol molecules surrounding the macromolecular chains led to a decrease in the swelling degree and an increase in the WVP along with improvements in mechanical properties.
{"title":"Development of Biodegradable Films Based on Chitosan/Glycerol Blends Suitable for Biomedical Applications","authors":"Deb, I. Maria, C. Bernal, N. Francois","doi":"10.4172/2157-7552.1000187","DOIUrl":"https://doi.org/10.4172/2157-7552.1000187","url":null,"abstract":"In the present work, chitosan/glycerol blends were used to prepare biodegradable films with potential application in dermal treatment. While the presence of glycerol gives the hydrating effect, the chitosan characteristics contribute to dermal regeneration. Swelling degree measurements, water vapor permeability determinations, mechanical tests, scanning electronic microscopy and Fourier transform infrared spectroscopy analysis; biodegradability studies, thermogravimetric and differential scanning calorimetry analysis were used to analyze the influence of the incorporation of significant amounts of glycerol in film properties. The obtained results suggested that the glycerol addition provide the films with the required properties for the intended application related to dermal treatment. Increasing the glycerol concentration, a cluster effect produced by the glycerol molecules surrounding the macromolecular chains led to a decrease in the swelling degree and an increase in the WVP along with improvements in mechanical properties.","PeriodicalId":17539,"journal":{"name":"Journal of Tissue Science and Engineering","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2016-12-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"80834565","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2016-11-30DOI: 10.4172/2157-7552.1000186
S. Shrivastava, C. Mahakalkar, P. Singh, A. Chandak, S. Tayde
Diabetic non-healing Ulcers are very difficult to treat. Many of such Patients undergo Amputations to lessen the morbidity. There is no defined management for them. Current management requires multiple surgical interventions, drugs and intense local dressing and care. The authors have developed an innovative therapy called as STARS (Sandeep’s technique for assisted regeneration of skin) for wound management. It is basically based on principle of regenerative medicine with autologous platelet rich Plasma (PRP) being the source of regenerative growth factors leading to complete healing of wounds. This case is a young female, a Juvenile Diabetic with non-healing infected ulcers on both heels since 3 years. She received STARS therapy and her one ulcer healed completely and second ulcer healed 40%. A complete healing of non healing diabetic ulcer without surgery, drugs and intense dressing, in an uncontrolled diabetic patient, is a milestone in such wound management. STARS therapy led by Platelet regeneration, is an innovative protocol for such cases.
{"title":"Platelet Rich Plasma as a Mono Therapy for Diabetic Ulcer","authors":"S. Shrivastava, C. Mahakalkar, P. Singh, A. Chandak, S. Tayde","doi":"10.4172/2157-7552.1000186","DOIUrl":"https://doi.org/10.4172/2157-7552.1000186","url":null,"abstract":"Diabetic non-healing Ulcers are very difficult to treat. Many of such Patients undergo Amputations to lessen the morbidity. There is no defined management for them. Current management requires multiple surgical interventions, drugs and intense local dressing and care. The authors have developed an innovative therapy called as STARS (Sandeep’s technique for assisted regeneration of skin) for wound management. It is basically based on principle of regenerative medicine with autologous platelet rich Plasma (PRP) being the source of regenerative growth factors leading to complete healing of wounds. This case is a young female, a Juvenile Diabetic with non-healing infected ulcers on both heels since 3 years. She received STARS therapy and her one ulcer healed completely and second ulcer healed 40%. A complete healing of non healing diabetic ulcer without surgery, drugs and intense dressing, in an uncontrolled diabetic patient, is a milestone in such wound management. STARS therapy led by Platelet regeneration, is an innovative protocol for such cases.","PeriodicalId":17539,"journal":{"name":"Journal of Tissue Science and Engineering","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2016-11-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"81373678","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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