This study aimed to eveluate effect of combination powder of red ginger (Zingiber officinale var. Rubrum), turmeric (Curcuma domestica), and temulawak (Curcuma xanthorrhiza) on the calculation of normal Liberkhun crypts by insilico and invivo methods. Molecular docking conducted in the pocket site of Interferon Lambda Receptor (IFN-λR) to modulate anti-inflammatory activity in chicken caecum. The study used 20 day old chickens (DOC) which were randomly grouped into 5 groups: negative control without treatment (P0) and powder addition group {0,5% (P1); 1%(P2); 1.5%(P3); 2%(P4)}. All chickens were reared for 35 days then chickens were euthanized using halal slaughter method and caecum organs were collected for histopathological observations. all active substances red ginger (gingerol, shogaol); turmeric (curcumin,tetrahydrocurcumin); and temulawak (demethoxycurcumin, bisdemethoxycurcumin) can bind to IFN-λR. Curcumin (-6.0 kcal/mol) have highest affinity values. Invivo treatment showed significant effect (p<0.05) on normal calculation of Liberkhun crypts due to tissue damage. Tukey's test confirmed that negative group was significantly different from P2, P3, and P4 with an average (4.35 ± 1.09b; 5.6 ± 1.54c; and 5.7 ± 1.78c). This research suggests that the addition of combination powder by 1,5% can potentially used for anti-inflamatory agent that confirmed by insilico method.
{"title":"Analisis Pemberian Serbuk Jahe Merah, Kunyit, dan Temulawak Dengan Metode Insilico dan Invivo pada Ayam Broiler","authors":"H. ., Renanda Nur Al Jabbar, Yudit Oktanella","doi":"10.22146/jsv.76111","DOIUrl":"https://doi.org/10.22146/jsv.76111","url":null,"abstract":"This study aimed to eveluate effect of combination powder of red ginger (Zingiber officinale var. Rubrum), turmeric (Curcuma domestica), and temulawak (Curcuma xanthorrhiza) on the calculation of normal Liberkhun crypts by insilico and invivo methods. Molecular docking conducted in the pocket site of Interferon Lambda Receptor (IFN-λR) to modulate anti-inflammatory activity in chicken caecum. The study used 20 day old chickens (DOC) which were randomly grouped into 5 groups: negative control without treatment (P0) and powder addition group {0,5% (P1); 1%(P2); 1.5%(P3); 2%(P4)}. All chickens were reared for 35 days then chickens were euthanized using halal slaughter method and caecum organs were collected for histopathological observations. all active substances red ginger (gingerol, shogaol); turmeric (curcumin,tetrahydrocurcumin); and temulawak (demethoxycurcumin, bisdemethoxycurcumin) can bind to IFN-λR. Curcumin (-6.0 kcal/mol) have highest affinity values. Invivo treatment showed significant effect (p<0.05) on normal calculation of Liberkhun crypts due to tissue damage. Tukey's test confirmed that negative group was significantly different from P2, P3, and P4 with an average (4.35 ± 1.09b; 5.6 ± 1.54c; and 5.7 ± 1.78c). This research suggests that the addition of combination powder by 1,5% can potentially used for anti-inflamatory agent that confirmed by insilico method.","PeriodicalId":17708,"journal":{"name":"Jurnal Sain Veteriner","volume":"17 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-04-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"76828738","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
R. A. Primatika, Bambang Sumiarto, Dyah Ayu Widiasih, Yatri Drastini, Heru Susetya, W. S. Nugroho, M. Putri
African Swine Fever (ASF) is an infectious disease in pigs caused by the African Swine Fever virus (ASFV) with virus species in the Asfarviridae family and Asfivirus genus. African Swine Fever causes economic losses for pig farmers with the number of deaths until 100%. This study aims to determine the distribution of ASF disease directions in Dairi Regency, North Sumatra Province and clusters and outliers in ASF cases in 2019 and 2020. This research method uses cartographic analysis, namely the distribution of directions and clusters and outliers with Moran's Index. Software using ArcGIS 10.5. This study obtained the results that the distribution of ASF disease in Dairi Regency in 2019 had an average center in Siempat Nempu Hulu District with longitude coordinates of 98.265459 and latitude 2.8055450. The distribution of ASF cases in 2019 is in the northwest – southeast region with a rotation of 121.05°. The ASF case in 2020 has a mean center in Lae Parira District with coordinates of longitude 98.3998 and latitude 2.7367. Meanwhile, the distribution of ASF cases in 2020 is northwest – southeast with a rotation of 133.58°. The conclusion of this study can be seen that cases of swine mortality due to ASF virus have a pattern of distribution of ASF disease and ASF case groups in 2019 and 2020.
{"title":"ANALISIS KARTOGRAFI KASUS WABAH AFRICAN SWINE FEVER DI KABUPATEN DAIRI PROVINSI SUMATERA UTARA","authors":"R. A. Primatika, Bambang Sumiarto, Dyah Ayu Widiasih, Yatri Drastini, Heru Susetya, W. S. Nugroho, M. Putri","doi":"10.22146/jsv.70218","DOIUrl":"https://doi.org/10.22146/jsv.70218","url":null,"abstract":"African Swine Fever (ASF) is an infectious disease in pigs caused by the African Swine Fever virus (ASFV) with virus species in the Asfarviridae family and Asfivirus genus. African Swine Fever causes economic losses for pig farmers with the number of deaths until 100%. This study aims to determine the distribution of ASF disease directions in Dairi Regency, North Sumatra Province and clusters and outliers in ASF cases in 2019 and 2020. This research method uses cartographic analysis, namely the distribution of directions and clusters and outliers with Moran's Index. Software using ArcGIS 10.5. This study obtained the results that the distribution of ASF disease in Dairi Regency in 2019 had an average center in Siempat Nempu Hulu District with longitude coordinates of 98.265459 and latitude 2.8055450. The distribution of ASF cases in 2019 is in the northwest – southeast region with a rotation of 121.05°. The ASF case in 2020 has a mean center in Lae Parira District with coordinates of longitude 98.3998 and latitude 2.7367. Meanwhile, the distribution of ASF cases in 2020 is northwest – southeast with a rotation of 133.58°. The conclusion of this study can be seen that cases of swine mortality due to ASF virus have a pattern of distribution of ASF disease and ASF case groups in 2019 and 2020.","PeriodicalId":17708,"journal":{"name":"Jurnal Sain Veteriner","volume":"113 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-04-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"75866182","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Heris Kustiningsih, E. Sudarnika, A. Saleh, C. Basri, M. Sudarwanto
Brucellosis is an infectious and zoonotic disease that causes a negative impact on the health and economy of people in almost all countries. The prevalence of brucellosis in several regions in Indonesia is still quite high. The purpose of this study was to analyze the role of farmers in the brucellosis control and surveillance program in Bogor Regency. The study was conducted through a survey of 151 farmers in Bogor Regency from May to July 2022. A total of 68.9% of respondents are aged 25-50 years with an education level of 69.8% are low educated (Elementary School, Junior High School) and not in school . The experience of raising more than five years (58.2%) with the number of dairy cattle ownership is less than five heads, namely 41.7%. The brucellosis prevention practices that need to be improved are related to the use of disinfectants when cleaning the cage, livestock group management practices (separating pregnant cows from other cows, separating cows with suspected brucellosis from other healthy cows, separating newly purchased cows for two weeks before being merged with cows). long in the cage). The practice of brucellosis control that needs to be improved is proper handling of the placenta and cows infected with brucellosis properly. Surveillance practices that need to be improved are the practice of recording and reporting cases of miscarriage in the final trimester. After identifying farmer practices, it is very important to plan interventions in the form of training with a curriculum that refers to the level of farmer practice that is still lacking. It is hoped that by conducting training interventions for farmers, the level of knowledge of farmers will increase and the level of practice of farmers will also increase.
{"title":"The Role of Dairy Farmers in Surveillance and Control Program of Brucellosis in Bogor Regency","authors":"Heris Kustiningsih, E. Sudarnika, A. Saleh, C. Basri, M. Sudarwanto","doi":"10.22146/jsv.77617","DOIUrl":"https://doi.org/10.22146/jsv.77617","url":null,"abstract":"Brucellosis is an infectious and zoonotic disease that causes a negative impact on the health and economy of people in almost all countries. The prevalence of brucellosis in several regions in Indonesia is still quite high. The purpose of this study was to analyze the role of farmers in the brucellosis control and surveillance program in Bogor Regency. The study was conducted through a survey of 151 farmers in Bogor Regency from May to July 2022. A total of 68.9% of respondents are aged 25-50 years with an education level of 69.8% are low educated (Elementary School, Junior High School) and not in school . The experience of raising more than five years (58.2%) with the number of dairy cattle ownership is less than five heads, namely 41.7%. The brucellosis prevention practices that need to be improved are related to the use of disinfectants when cleaning the cage, livestock group management practices (separating pregnant cows from other cows, separating cows with suspected brucellosis from other healthy cows, separating newly purchased cows for two weeks before being merged with cows). long in the cage). The practice of brucellosis control that needs to be improved is proper handling of the placenta and cows infected with brucellosis properly. Surveillance practices that need to be improved are the practice of recording and reporting cases of miscarriage in the final trimester. After identifying farmer practices, it is very important to plan interventions in the form of training with a curriculum that refers to the level of farmer practice that is still lacking. It is hoped that by conducting training interventions for farmers, the level of knowledge of farmers will increase and the level of practice of farmers will also increase.","PeriodicalId":17708,"journal":{"name":"Jurnal Sain Veteriner","volume":"12 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"87567833","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Kurnia Desiandura, I. P. Hermawan, Hana Cipka Pramuda Wardhani, Era Hari Mudji Restijono, Palestin .
Abstract Background: The causes of cases of Acute Moist Dermatitis (AMD) or also known as hotspots are numerous. Early AMD is accompanied by symptoms of pruritus or different behaviors triggered by itching such as scratching or licking. Many causes of pruritus in cats such as hypersensitivity dermatitis, ectoparasites, fungal infections, bacterial infections, or skin reactions to systemic diseases. Gradually clinical symptoms will occur alopecia and erythema because it is very itchy, moist and smells on the surface of the skin. Case Description: A male Persian cat, 1.6 years old and weighing 3.4 kg, presented with a history of pruritus, alopecia, erythema and wet skin in several locations, especially around the neck, and had been present for 3 months. Appetite to eat and drink is not very good because the cat is busy scratching and biting its fur due to excessive itching. Examination Results and Treatments: After clinical examination and microscopic examination of samples, the main trigger in this case was furmite in a cat, Lynxacarus radovskyi with secondary bacterial infection causing Acute Moist Dermatitis (AMD). Not only that, ectoparasites in this case also cause other systemic diseases, namely suspicion of blood parasites that cause thrombocytopenia as evidenced by the results of a Complete Blood Count (CBC), and is characterized by clinical symptoms of hematuria and epistaxis. This cat underwent intensive treatment for several weeks with several combinations of drugs such as antiparasitics, antibiotics. antihistamines, NSID and vitamins. Conclusion: This
{"title":"Acute Moist Dermatitis with Thrombocytopenia in Cat","authors":"Kurnia Desiandura, I. P. Hermawan, Hana Cipka Pramuda Wardhani, Era Hari Mudji Restijono, Palestin .","doi":"10.22146/jsv.75944","DOIUrl":"https://doi.org/10.22146/jsv.75944","url":null,"abstract":"Abstract Background: The causes of cases of Acute Moist Dermatitis (AMD) or also known as hotspots are numerous. Early AMD is accompanied by symptoms of pruritus or different behaviors triggered by itching such as scratching or licking. Many causes of pruritus in cats such as hypersensitivity dermatitis, ectoparasites, fungal infections, bacterial infections, or skin reactions to systemic diseases. Gradually clinical symptoms will occur alopecia and erythema because it is very itchy, moist and smells on the surface of the skin. Case Description: A male Persian cat, 1.6 years old and weighing 3.4 kg, presented with a history of pruritus, alopecia, erythema and wet skin in several locations, especially around the neck, and had been present for 3 months. Appetite to eat and drink is not very good because the cat is busy scratching and biting its fur due to excessive itching. Examination Results and Treatments: After clinical examination and microscopic examination of samples, the main trigger in this case was furmite in a cat, Lynxacarus radovskyi with secondary bacterial infection causing Acute Moist Dermatitis (AMD). Not only that, ectoparasites in this case also cause other systemic diseases, namely suspicion of blood parasites that cause thrombocytopenia as evidenced by the results of a Complete Blood Count (CBC), and is characterized by clinical symptoms of hematuria and epistaxis. This cat underwent intensive treatment for several weeks with several combinations of drugs such as antiparasitics, antibiotics. antihistamines, NSID and vitamins. Conclusion: This","PeriodicalId":17708,"journal":{"name":"Jurnal Sain Veteriner","volume":"29 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"84675711","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Penelitian ini bertujuan untuk mengetahui efektivitas pemberian salep ekstrak etanol daun kelor 20% (EEDK 20%) pada penyembuhan luka berdasarkan gambaran histopatologi yang meliputi jumlah leukosit pada jaringan luka dan ketebalan epitel. Enam belas ekor tikus Sprague-Dawley betina, umur 3-4 bulan dengan berat 150-200 g, dibagi menjadi 4 kelompok perlakuan (A,B,C dan D) secara acak. Seluruh tikus diovariektomi dan diberi perlakuan pakan selama 8 minggu. Kelompok A dan C diberi pakan normal, sedangkan kelompok B dan D diberi pakan tinggi lemak. Setelah 8 minggu, dilakukan pengambilan darah untuk analisis kadar lemak (kolesterol dan trigliserida) dan pembuatan luka di area punggung dengan biopsy punch (8 mm). Perawatan dilakukan dengan pemberian salep selama 7 hari dan injeksi antibiotik. Kelompok A dan B diberi salep basis, sedangkan C dan D diberi salep EEDK 20%. Hari ke-7 dilakukan pengambilan jaringan kulit untuk analisis histopatologi dengan pengecatan Hematoksilin Eosin. Kadar lemak darah (kolesterol dan trigliserida) dianalisis secara statistik dengan one way ANOVA, sedangkan jumlah leukosit di jaringan dan ketebalan epitel dianalisis dengan two way ANOVA. Hasil penelitian menunjukkan bahwa jumlah leukosit di jaringan dipengaruhi oleh jenis salep (P<0.05), sedangkan ketebalan epitel dipengaruhi oleh jenis salep dan pakan (P<0,05). Salep EEDK 20% dapat menurunkan jumlah leukosit di jaringan, meningkatkan ketebalan epitel dan memiliki gambaran histopatologi kulit yang baik. Dapat disimpulkan bahwa pemberian salep EEDK 20% dapat meningkatkan proses penyembuhan luka tikus yang diovariektomi dan diberi pakan tinggi lemak. Kata kunci : daun kelor; luka; penyembuhan; salep EEDK 20%; tinggi lemak
{"title":"Pemanfaatan Ekstrak Daun Kelor (Moringa oleifera lamk) untuk Penyembuhan Luka Tikus Ovariektomi yang Diberi Diet Tinggi Lemak","authors":"S. Fadillah, Dhirgo Aji, Devita Anggraeni","doi":"10.22146/jsv.77161","DOIUrl":"https://doi.org/10.22146/jsv.77161","url":null,"abstract":"Penelitian ini bertujuan untuk mengetahui efektivitas pemberian salep ekstrak etanol daun kelor 20% (EEDK 20%) pada penyembuhan luka berdasarkan gambaran histopatologi yang meliputi jumlah leukosit pada jaringan luka dan ketebalan epitel. Enam belas ekor tikus Sprague-Dawley betina, umur 3-4 bulan dengan berat 150-200 g, dibagi menjadi 4 kelompok perlakuan (A,B,C dan D) secara acak. Seluruh tikus diovariektomi dan diberi perlakuan pakan selama 8 minggu. Kelompok A dan C diberi pakan normal, sedangkan kelompok B dan D diberi pakan tinggi lemak. Setelah 8 minggu, dilakukan pengambilan darah untuk analisis kadar lemak (kolesterol dan trigliserida) dan pembuatan luka di area punggung dengan biopsy punch (8 mm). Perawatan dilakukan dengan pemberian salep selama 7 hari dan injeksi antibiotik. Kelompok A dan B diberi salep basis, sedangkan C dan D diberi salep EEDK 20%. Hari ke-7 dilakukan pengambilan jaringan kulit untuk analisis histopatologi dengan pengecatan Hematoksilin Eosin. Kadar lemak darah (kolesterol dan trigliserida) dianalisis secara statistik dengan one way ANOVA, sedangkan jumlah leukosit di jaringan dan ketebalan epitel dianalisis dengan two way ANOVA. Hasil penelitian menunjukkan bahwa jumlah leukosit di jaringan dipengaruhi oleh jenis salep (P<0.05), sedangkan ketebalan epitel dipengaruhi oleh jenis salep dan pakan (P<0,05). Salep EEDK 20% dapat menurunkan jumlah leukosit di jaringan, meningkatkan ketebalan epitel dan memiliki gambaran histopatologi kulit yang baik. Dapat disimpulkan bahwa pemberian salep EEDK 20% dapat meningkatkan proses penyembuhan luka tikus yang diovariektomi dan diberi pakan tinggi lemak. Kata kunci : daun kelor; luka; penyembuhan; salep EEDK 20%; tinggi lemak","PeriodicalId":17708,"journal":{"name":"Jurnal Sain Veteriner","volume":"162 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"86189011","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Elisabet Tangkonda, Antin Yeftanti Nugrahening Widi, Bergita Soge
Snot adalah salah satu gejala infeksi saluran pernafasan atas pada ayam, yang biasanya ditandai oleh kesulitan bernafas, eksudat dari rongga hidung, pembengkakan sinus infraorbital, ngorok, dan bersin. Meskipun seringkali dihubungkan dengan snot, tidak semua kejadian penyakit dengan gejala snot disebabkan oleh Avibaterium paragallinarum. Penyakit unggas dengan gejala snot sulit untuk diberantas karena manajemen peternakan yang kurang baik, variasi umur unggas dalam peternakan yang sama, fluktuasi suhu, dan kelembaban yang lebih tinggi, seperti yang sering ditemui Indonesia. Penyakit unggas dengan gejala snot telah dilaporkan dari berbagai provinsi di Indonesia. Penelitian ini bertujuan untuk mengisolasi dan mengidentifikasi bakteri penyebab snot pada ayam broiler di Kabupaten Kupang. Sebanyak 4 sampel swab eksudat sinus infraorbital dan trakea dari unggas yang menunjukkan gejala khas snot diambil dari 2 peternakan broiler komersial di Kabupaten Kupang, dikultur pada agar darah dan agar coklat (disiapkan dengan 5% darah domba), dan diinkubasi pada suhu 37ºC selama 18-24 jam pada kondisi aerob dan anaerob. Sejumlah 23 isolat dimurnikan pada media nutrient agar kemudian dilanjutkan dengan untuk uji biokimia (katalase, oksidase, uji motilitas dan produksi indol) dan uji fermentasi gula. Hasil pengamatan menunjukkan, dari 23 isolat yang diuji, 17 isolat (73,91%) teridentifikasi sebagai Avibacterium paragallinarum (4 isolat), Ornithobacterium rhinotracheale (4 isolat), Pasteurella multocida (3 isolat) dan Mycoplasma sp. (6 isolat), sedangkan 6 isolat (26,09%) tidak teridentifikasi.. Dapat disimpulkan bahwa Avibacterium paragallinarum, Ornithobacterium rhinotracheale, Pasteurella multocida, dan Mycoplasma sp adalah penyebab gejala snot pada ayam broiler di Kabupaten Kupang.
{"title":"Isolasi dan Identifikasi Agen Etiologi Gejala Snot pada Ayam Broiler di Kabupaten Kupang","authors":"Elisabet Tangkonda, Antin Yeftanti Nugrahening Widi, Bergita Soge","doi":"10.22146/jsv.75848","DOIUrl":"https://doi.org/10.22146/jsv.75848","url":null,"abstract":"Snot adalah salah satu gejala infeksi saluran pernafasan atas pada ayam, yang biasanya ditandai oleh kesulitan bernafas, eksudat dari rongga hidung, pembengkakan sinus infraorbital, ngorok, dan bersin. Meskipun seringkali dihubungkan dengan snot, tidak semua kejadian penyakit dengan gejala snot disebabkan oleh Avibaterium paragallinarum. Penyakit unggas dengan gejala snot sulit untuk diberantas karena manajemen peternakan yang kurang baik, variasi umur unggas dalam peternakan yang sama, fluktuasi suhu, dan kelembaban yang lebih tinggi, seperti yang sering ditemui Indonesia. Penyakit unggas dengan gejala snot telah dilaporkan dari berbagai provinsi di Indonesia. Penelitian ini bertujuan untuk mengisolasi dan mengidentifikasi bakteri penyebab snot pada ayam broiler di Kabupaten Kupang. Sebanyak 4 sampel swab eksudat sinus infraorbital dan trakea dari unggas yang menunjukkan gejala khas snot diambil dari 2 peternakan broiler komersial di Kabupaten Kupang, dikultur pada agar darah dan agar coklat (disiapkan dengan 5% darah domba), dan diinkubasi pada suhu 37ºC selama 18-24 jam pada kondisi aerob dan anaerob. Sejumlah 23 isolat dimurnikan pada media nutrient agar kemudian dilanjutkan dengan untuk uji biokimia (katalase, oksidase, uji motilitas dan produksi indol) dan uji fermentasi gula. Hasil pengamatan menunjukkan, dari 23 isolat yang diuji, 17 isolat (73,91%) teridentifikasi sebagai Avibacterium paragallinarum (4 isolat), Ornithobacterium rhinotracheale (4 isolat), Pasteurella multocida (3 isolat) dan Mycoplasma sp. (6 isolat), sedangkan 6 isolat (26,09%) tidak teridentifikasi.. Dapat disimpulkan bahwa Avibacterium paragallinarum, Ornithobacterium rhinotracheale, Pasteurella multocida, dan Mycoplasma sp adalah penyebab gejala snot pada ayam broiler di Kabupaten Kupang.","PeriodicalId":17708,"journal":{"name":"Jurnal Sain Veteriner","volume":"45 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"77521849","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Christin Melkianus, Hary Purnamaningsih, Yanuartono Yanuartono, I. Widiyono
Tujuan dari penelitian ini adalah mengkaji pengaruh aplikasi gliserol secara oral drenching terhadap kadar glukosa darah pada kambing Kacang (Capra aegagrus hircus). Sebanyak 7 ekor kambing Kacang betina, umur ± 2 tahun, bobot badan 26 kg, kondisi tubuh BCS (2,5–3), tidak bunting, dan secara klinis sehat. Hewan dibagi menjadi 2 kelompok yakni kelompok perlakuan dengan larutan gliserol (G) dan kelompok perlakuan dengan air (A) sebagai kontrol. Larutan gliserol dibuat dengan melarutkan gliserol dalam air dengan perbandingan 1:1. Setelah melampaui masa adaptasi sekitar satu bulan, setiap hewan pada kelompok G diberi 2 kali perlakuan oral drenching larutan gliserol dengan dosis 4 ml/kg BB (G2) dan 8 ml/kg BB (G4), sedang setiap hewan kelompok A diberi air dengan dosis 4 ml/kg BB (A4) dan 8 ml/kg BB (A8). Pelaksanaan kedua perlakuan pada setiap hewan berselang 3 pekan. Sampel darah diambil sebelum (pada menit ke-0) dan pada menit ke-60, 120, dan 180 menit setelah oral drencing. Data yang diperoleh dianalisis dengan menggunakan ANOVA. P<0,05 ditentukan sebagai kriteria signifikansi. Hasil dari penelitian menunjukkan bahwa pemberian gliserol pada kelompok G2 dan G4 mengakibatkan peningkatan yang signifikan pada jam kedua dan belum menunjukkan penurunan sampai menit ke 180 yakni berada pada level 80.75 g/dL dan 88.30 mg/dL (P<0,05), sedang pemberian air dengan dosis yang sama pada kelompok A4 dan A8 tidak mengakibatkan perubahan kadar glukosa darah secara signifikan dan berada pada kisaran 58-66 mg/dL (P>0,05). Berdasarkan hasil penelitian tersebut dapat disimpulkan bahwan pemberian gliserol secara oral drenching dengan dosis sampai 2-4 ml/kg BB pada kambing Kacang potensial meningkatkan kadar glukosa darah secara cepat dan bertahan tidak kurang dari 3 jam setelah pemberian.
{"title":"Pemberian Gliserol secara Oral dengan Dosis 2-4 Ml/Kg Berat Badan Meningkatkan Kadar Fisiologik Glukosa Darah: Kajian pada Kambing Kacang (Capra Aegagrus Hircus)","authors":"Christin Melkianus, Hary Purnamaningsih, Yanuartono Yanuartono, I. Widiyono","doi":"10.22146/jsv.75525","DOIUrl":"https://doi.org/10.22146/jsv.75525","url":null,"abstract":"Tujuan dari penelitian ini adalah mengkaji pengaruh aplikasi gliserol secara oral drenching terhadap kadar glukosa darah pada kambing Kacang (Capra aegagrus hircus). Sebanyak 7 ekor kambing Kacang betina, umur ± 2 tahun, bobot badan 26 kg, kondisi tubuh BCS (2,5–3), tidak bunting, dan secara klinis sehat. Hewan dibagi menjadi 2 kelompok yakni kelompok perlakuan dengan larutan gliserol (G) dan kelompok perlakuan dengan air (A) sebagai kontrol. Larutan gliserol dibuat dengan melarutkan gliserol dalam air dengan perbandingan 1:1. Setelah melampaui masa adaptasi sekitar satu bulan, setiap hewan pada kelompok G diberi 2 kali perlakuan oral drenching larutan gliserol dengan dosis 4 ml/kg BB (G2) dan 8 ml/kg BB (G4), sedang setiap hewan kelompok A diberi air dengan dosis 4 ml/kg BB (A4) dan 8 ml/kg BB (A8). Pelaksanaan kedua perlakuan pada setiap hewan berselang 3 pekan. Sampel darah diambil sebelum (pada menit ke-0) dan pada menit ke-60, 120, dan 180 menit setelah oral drencing. Data yang diperoleh dianalisis dengan menggunakan ANOVA. P<0,05 ditentukan sebagai kriteria signifikansi. Hasil dari penelitian menunjukkan bahwa pemberian gliserol pada kelompok G2 dan G4 mengakibatkan peningkatan yang signifikan pada jam kedua dan belum menunjukkan penurunan sampai menit ke 180 yakni berada pada level 80.75 g/dL dan 88.30 mg/dL (P<0,05), sedang pemberian air dengan dosis yang sama pada kelompok A4 dan A8 tidak mengakibatkan perubahan kadar glukosa darah secara signifikan dan berada pada kisaran 58-66 mg/dL (P>0,05). Berdasarkan hasil penelitian tersebut dapat disimpulkan bahwan pemberian gliserol secara oral drenching dengan dosis sampai 2-4 ml/kg BB pada kambing Kacang potensial meningkatkan kadar glukosa darah secara cepat dan bertahan tidak kurang dari 3 jam setelah pemberian.","PeriodicalId":17708,"journal":{"name":"Jurnal Sain Veteriner","volume":"75 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"77067006","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Asmarani Kusumawati, Lalu Unsunnidhal, Agung Budiyanto, Erif Maha Nugraha Setyawan, Sri Gustari
Virus Penyakit Jembrana adalah virus yang menyebabkan jembrana pada sapi bali. Vaksin protein rekombinan dibuat dengan memasukkan DNA atau gen yang mengkode protein imunogenik ke dalam vektor ekspresi prokariotik sehingga dapat mengekspresikan protein rekombinan yang akan dijadikan vaksin. Salah satu gen struktural virus penyakit jembrana adalah gen tat yang mengekspresikan protein tat yang digunakan sebagai vaksin protein rekombinan. Tujuan dari penelitian ini adalah untuk mengkloning pET15b dengan gen tat. Konfirmasi gen pET-15b pada bakteri transforman dilakukan dengan koloni PCR, kemudian dilakukan isolasi plasmid dan dilaksanakan konfirmasi menggunakan metode PCR dengan primer untuk deteksi gen tat dan primer sekuensing untuk vektor pET15b. Hasilnya menunjukkan bahwa bakteri transforman sudah berisikan DNA rekombinan pET15b-tat yang terkonfirmasi melalui koloni PCR dengan band sebesar 179 bp, kemudian dikonfirmasi lanjut melalui PCR pada hasil isolasi plasmid dari bakteri transforman yang didapatkan band sebesar 179 bp untuk primer deteksi gen tat dan 514 bp untuk primer sekuensing pada vektor pET15b, 179 bp dan 514 bp sesuai dengan target amplikasi untuk primer deteksi dan primer sekuensing yang digunakan.
Jembrana病毒是导致巴厘岛牛患Jembrana的病毒。重组蛋白疫苗是通过将免疫蛋白编码的DNA或基因输入原核表达媒介，从而表达将被制成疫苗的重组蛋白。jembrana病毒的结构基因之一是tat基因，它表达了一种用作蛋白质重组蛋白疫苗的tat蛋白。本研究的目的是用tat基因克隆pET15b。确认transforman细菌上的pET-15b基因与PCR菌落有关，然后通过PCR的原发性方法进行分离和确认，使用PCR的原始方法检测tat的基因和对pET15b的主序列。结果表明,细菌transforman已经确认的重组DNA包含pET15b-tat乐队通过PCR殖民地179万英国石油(bp),然后通过PCR的结果确认进一步孤立bp transforman乐队的179万细菌的质粒为初级tat和514 bp基因检测主要pET15b载体上,179 bp (bp)和514测序检测符合初级amplikasi目标的方向和主要使用的测序。
{"title":"Konstruksi Plasmid pET-15b dengan Gen tat Virus Penyakit Jembrana","authors":"Asmarani Kusumawati, Lalu Unsunnidhal, Agung Budiyanto, Erif Maha Nugraha Setyawan, Sri Gustari","doi":"10.22146/jsv.79217","DOIUrl":"https://doi.org/10.22146/jsv.79217","url":null,"abstract":"Virus Penyakit Jembrana adalah virus yang menyebabkan jembrana pada sapi bali. Vaksin protein rekombinan dibuat dengan memasukkan DNA atau gen yang mengkode protein imunogenik ke dalam vektor ekspresi prokariotik sehingga dapat mengekspresikan protein rekombinan yang akan dijadikan vaksin. Salah satu gen struktural virus penyakit jembrana adalah gen tat yang mengekspresikan protein tat yang digunakan sebagai vaksin protein rekombinan. Tujuan dari penelitian ini adalah untuk mengkloning pET15b dengan gen tat. Konfirmasi gen pET-15b pada bakteri transforman dilakukan dengan koloni PCR, kemudian dilakukan isolasi plasmid dan dilaksanakan konfirmasi menggunakan metode PCR dengan primer untuk deteksi gen tat dan primer sekuensing untuk vektor pET15b. Hasilnya menunjukkan bahwa bakteri transforman sudah berisikan DNA rekombinan pET15b-tat yang terkonfirmasi melalui koloni PCR dengan band sebesar 179 bp, kemudian dikonfirmasi lanjut melalui PCR pada hasil isolasi plasmid dari bakteri transforman yang didapatkan band sebesar 179 bp untuk primer deteksi gen tat dan 514 bp untuk primer sekuensing pada vektor pET15b, 179 bp dan 514 bp sesuai dengan target amplikasi untuk primer deteksi dan primer sekuensing yang digunakan.","PeriodicalId":17708,"journal":{"name":"Jurnal Sain Veteriner","volume":"37 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"75561861","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Maria Yohana Simarmata, Rantimah ., Syafri Edwar, Kusdiantoro Mohamad
Singa afrika merupakan salah satu hewan yang dikategorikan rentan terhadap kepunahan (vulnerable) menurut IUCN. Kebun Binatang Ragunan merupakan lembaga konservasi ex-situ yang memiliki peran penting dalam upaya konservasi satwa liar. Seekor singa afrika di Taman Margasatwa Ragunan bernama Suki, berumur ± 14 tahun, betina, dilaporkan mengalami pembengkakan di telinga kanannya. Pembengkakan sudah berlangsung sekitar seminggu dan singa sering menggosokkan telinganya ke dinding kandang. Hasil pemeriksaan menunjukkan singa tersebut menderita auricular hematoma (othematoma). Perawatan pilihan adalah metode bedah dengan sayatan linier untuk menghilangkan cairan hematoma. Singa Suki diberi antibiotik, antiradang, dan multivitamin untuk perawatan pascabedah. Auricular hematoma pada singa Suki mengalami proses penyembuhan yang lambat dan sembuh setelah 47 hari pascabedah.
{"title":"Laporan Kasus Auricular Hematoma pada Singa Afrika (Panthera leo) di Taman Margasatwa Ragunan","authors":"Maria Yohana Simarmata, Rantimah ., Syafri Edwar, Kusdiantoro Mohamad","doi":"10.22146/jsv.75951","DOIUrl":"https://doi.org/10.22146/jsv.75951","url":null,"abstract":"Singa afrika merupakan salah satu hewan yang dikategorikan rentan terhadap kepunahan (vulnerable) menurut IUCN. Kebun Binatang Ragunan merupakan lembaga konservasi ex-situ yang memiliki peran penting dalam upaya konservasi satwa liar. Seekor singa afrika di Taman Margasatwa Ragunan bernama Suki, berumur ± 14 tahun, betina, dilaporkan mengalami pembengkakan di telinga kanannya. Pembengkakan sudah berlangsung sekitar seminggu dan singa sering menggosokkan telinganya ke dinding kandang. Hasil pemeriksaan menunjukkan singa tersebut menderita auricular hematoma (othematoma). Perawatan pilihan adalah metode bedah dengan sayatan linier untuk menghilangkan cairan hematoma. Singa Suki diberi antibiotik, antiradang, dan multivitamin untuk perawatan pascabedah. Auricular hematoma pada singa Suki mengalami proses penyembuhan yang lambat dan sembuh setelah 47 hari pascabedah.","PeriodicalId":17708,"journal":{"name":"Jurnal Sain Veteriner","volume":"104 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"74670452","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Infrared thermal camera is an alternative and non-invasive method used to identify various physiological and pathological process related to body surface temperature changes. The aim of this research was to study the body surface temperature pattern based on the observed regios. Three horses were randomly selected as animal experiments. Thermal image data was collected at 17.00 – 19.00 WIB for five days. Images were retrieved from seven regios: head, thorax, abdomen, and four lower legs. The result of horse A’s surface temperature in five days on head, thorax, abdomen, front left leg, front right leg, rear left leg, and rear right leg regios were 33.42, 33.49, 33.56, 31.30, 31.48, 31. 33.14, 31.34 °C, respectively. The resulf of horse B’s surface tempereture were 33.55, 33.65, 33.64, 31.45, 31.35, 31.53, 31.48 °C, respectively. The resulf of horse C’s surface tempereture were 33.45, 33.55, 33.60, 31.37, 31.45, 31.34, 31.42 °C, respectively. The result showed that difference on horse A’s rear left leg (p<0.05) because of inflamation. Inflamation recovery process on horse A occured on the fifth day, indicated by a drop in temperature and a change in colors on thermal image. Color noises on some images were color difference between objects and poor color saturation. Color noises on thermal images did not affect the results of temperature interpretation.
{"title":"PEMETAAN POLA SUHU PERMUKAAN TUBUH KUDA MENGGUNAKAN KAMERA TERMAL INFRAMERAH","authors":"Koekoeh Santoso, Rivangga Yuda Hendika, Mokhamad Fakhrul Ulum, Amrozi ., Ridi Arif, A. Suprayogi, Kudang Boro Seminar","doi":"10.22146/jsv.66859","DOIUrl":"https://doi.org/10.22146/jsv.66859","url":null,"abstract":"Infrared thermal camera is an alternative and non-invasive method used to identify various physiological and pathological process related to body surface temperature changes. The aim of this research was to study the body surface temperature pattern based on the observed regios. Three horses were randomly selected as animal experiments. Thermal image data was collected at 17.00 – 19.00 WIB for five days. Images were retrieved from seven regios: head, thorax, abdomen, and four lower legs. The result of horse A’s surface temperature in five days on head, thorax, abdomen, front left leg, front right leg, rear left leg, and rear right leg regios were 33.42, 33.49, 33.56, 31.30, 31.48, 31. 33.14, 31.34 °C, respectively. The resulf of horse B’s surface tempereture were 33.55, 33.65, 33.64, 31.45, 31.35, 31.53, 31.48 °C, respectively. The resulf of horse C’s surface tempereture were 33.45, 33.55, 33.60, 31.37, 31.45, 31.34, 31.42 °C, respectively. The result showed that difference on horse A’s rear left leg (p<0.05) because of inflamation. Inflamation recovery process on horse A occured on the fifth day, indicated by a drop in temperature and a change in colors on thermal image. Color noises on some images were color difference between objects and poor color saturation. Color noises on thermal images did not affect the results of temperature interpretation. ","PeriodicalId":17708,"journal":{"name":"Jurnal Sain Veteriner","volume":"22 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"75733230","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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