R. Widayanti, Siti Naimah, Rahma Prihutami, Trini Susmiati
One of the native catfish to Indonesia is the baung fish (Hemibagrus). Baung fish are found in rivers in Sumatra, Kalimantan and Java. The catfish population is declining, thus conservation is needed to prevent extinction. To conduct an effective conservation efforts, molecular studies are needed to confirm the fish species from the three islands. Mitochondrial DNA is one of the markers that is often used to see the lineage and kinship of animals for conservation purposes. The purpose of this study is to determine the genetic diversity of the NADH Dehydrogenase Subunit I (ND1) gene catfish from each of these locations, can be used as a genetic marker. Samples were obtained from their natural habitat, namely Magelang (5), Palembang (3), Riau (2), Samarinda (2), 2 from Sintang, and 3 from Banjarmasin. The DNA of the fish sample was then isolated and then used as a template for amplification of DNA fragments using PCR techniques. Amplikon (PCR product) was then purified by gel extraction and then sequenced to determine the DNA sequence. The potential of DNA sequences as catfish genetic markers was proven by analyzing genetic diversity between species using the MEGA version 7.0 program (Kumar et al., 2016). The sequencing results 972 nucleotides composing the ND gene, and found differences in 268 nucleotide sites and 47 amino acid sites. Based on the nucleotide sequence of the ND1 gene, catfish from the Progo river (Magelang, Central Java), the Musi river (Palembang, Sumatra), the Kampar river (Riau), the Kapuas river (Sintang, Kalimantan), the Martapura river (Banjarmasin), the Mahakam river (Kalimantan) is included in the Hemibagrus sp.; catfish from the Elo river (Magelang, Central Java) belongs to the genus Mystus sp.; catfish from the Bengawan Solo river (Bojonegoro, East Java) belongs to the Pangasius sp.
印度尼西亚的本地鲶鱼之一是baung鱼(半鲶鱼)。Baung鱼在苏门答腊、加里曼丹和爪哇的河流中被发现。鲶鱼的数量正在下降，因此需要保护以防止灭绝。为了进行有效的保护工作，需要进行分子研究来确定三个岛屿的鱼类种类。线粒体DNA是一种标记，通常用于观察动物的血统和亲属关系，以保护动物。本研究的目的是确定鲶鱼NADH脱氢酶亚基I (ND1)基因在这些位点的遗传多样性，是否可以作为遗传标记。样本来自它们的自然栖息地，即马格朗(5只)、巨港(3只)、廖内(2只)、萨玛林达(2只)、辛塘(2只)和班贾玛辛(3只)。然后分离鱼样本的DNA，然后使用PCR技术作为扩增DNA片段的模板。扩增子(PCR产物)经凝胶萃取纯化，测序确定DNA序列。通过使用MEGA version 7.0程序分析物种间的遗传多样性，证明了DNA序列作为鲶鱼遗传标记的潜力(Kumar et al.， 2016)。测序结果显示，ND基因由972个核苷酸组成，发现268个核苷酸位点和47个氨基酸位点存在差异。根据ND1基因的核苷酸序列，来自Progo河(Magelang，中爪哇)、Musi河(Palembang，苏门答腊)、Kampar河(廖内省)、Kapuas河(Sintang, Kalimantan)、Martapura河(Banjarmasin)、Mahakam河(Kalimantan)的鲶鱼被包括在Hemibagrus sp.;来自Elo河(中爪哇Magelang)的鲶鱼属于Mystus属;本加万梭罗河(东爪哇Bojonegoro)鲶鱼属鲶鱼科。
{"title":"Study of Genetic Diversity of Native Indonesian Catfish Based on Nucleotide Sequences of the ND1 Gene","authors":"R. Widayanti, Siti Naimah, Rahma Prihutami, Trini Susmiati","doi":"10.22146/jsv.62755","DOIUrl":"https://doi.org/10.22146/jsv.62755","url":null,"abstract":"One of the native catfish to Indonesia is the baung fish (Hemibagrus). Baung fish are found in rivers in Sumatra, Kalimantan and Java. The catfish population is declining, thus conservation is needed to prevent extinction. To conduct an effective conservation efforts, molecular studies are needed to confirm the fish species from the three islands. Mitochondrial DNA is one of the markers that is often used to see the lineage and kinship of animals for conservation purposes. The purpose of this study is to determine the genetic diversity of the NADH Dehydrogenase Subunit I (ND1) gene catfish from each of these locations, can be used as a genetic marker. Samples were obtained from their natural habitat, namely Magelang (5), Palembang (3), Riau (2), Samarinda (2), 2 from Sintang, and 3 from Banjarmasin. The DNA of the fish sample was then isolated and then used as a template for amplification of DNA fragments using PCR techniques. Amplikon (PCR product) was then purified by gel extraction and then sequenced to determine the DNA sequence. The potential of DNA sequences as catfish genetic markers was proven by analyzing genetic diversity between species using the MEGA version 7.0 program (Kumar et al., 2016). The sequencing results 972 nucleotides composing the ND gene, and found differences in 268 nucleotide sites and 47 amino acid sites. Based on the nucleotide sequence of the ND1 gene, catfish from the Progo river (Magelang, Central Java), the Musi river (Palembang, Sumatra), the Kampar river (Riau), the Kapuas river (Sintang, Kalimantan), the Martapura river (Banjarmasin), the Mahakam river (Kalimantan) is included in the Hemibagrus sp.; catfish from the Elo river (Magelang, Central Java) belongs to the genus Mystus sp.; catfish from the Bengawan Solo river (Bojonegoro, East Java) belongs to the Pangasius sp.","PeriodicalId":17708,"journal":{"name":"Jurnal Sain Veteriner","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2022-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"78781550","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Jayanti Berliana Dewi, Delvi Ramadayani, Marchellia Dhiafifah Wahyunandha, Melati Kusuma Bunda Pertiwi, Sri Gustari, Topas Wicaksono Priyo Jr
One of the effort to provide superior rams is to pay attention to the provision of feed that has good substances and is able to improve the quality of sperm. Based on studies, snail flour has a fairly high protein content and snails can be an alternative source of protein in additional feed. This study aims to improve the quality of sheep sperm using an additional feed of snail flour (Achatina fulica) which can increase the use value and economic value. Eight sheep used in this research were divided into one group as the control and two groups as the treatment. The control group was given standard sheep forage, while the treatment groups were given standard sheep forage added with flour snail of 5% and 10%. Sperm collection using castration method. Parameters observed were motility, viability, and concentration of sperms. The results of the study showed that the addition of snail flour as additional feed for sheep affected sperm quality with a significant increase in concentration parameters, while motility and viability did not show a significant increase
{"title":"TEPUNG BEKICOT (Achatina fulica) SEBAGAI PAKAN TAMBAHAN UNTUK MENINGKATKAN KUALITAS SEMEN DOMBA (Ovis aries)","authors":"Jayanti Berliana Dewi, Delvi Ramadayani, Marchellia Dhiafifah Wahyunandha, Melati Kusuma Bunda Pertiwi, Sri Gustari, Topas Wicaksono Priyo Jr","doi":"10.22146/jsv.69114","DOIUrl":"https://doi.org/10.22146/jsv.69114","url":null,"abstract":"One of the effort to provide superior rams is to pay attention to the provision of feed that has good substances and is able to improve the quality of sperm. Based on studies, snail flour has a fairly high protein content and snails can be an alternative source of protein in additional feed. This study aims to improve the quality of sheep sperm using an additional feed of snail flour (Achatina fulica) which can increase the use value and economic value. Eight sheep used in this research were divided into one group as the control and two groups as the treatment. The control group was given standard sheep forage, while the treatment groups were given standard sheep forage added with flour snail of 5% and 10%. Sperm collection using castration method. Parameters observed were motility, viability, and concentration of sperms. The results of the study showed that the addition of snail flour as additional feed for sheep affected sperm quality with a significant increase in concentration parameters, while motility and viability did not show a significant increase","PeriodicalId":17708,"journal":{"name":"Jurnal Sain Veteriner","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2022-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"80999955","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
This study was aimed to identify the presence of E. coli O157:H7 and analyze the association between factors and frequency of E. coli in chicken meat sold at Kaget Market, Pagi Market, and Pembangunan Market in Pangkalpinang City. A total of 30 chicken meat samples were collected proportionally using simple random sampling. This study was used a cross sectional method. The examination of E. coli was carried out based on SNI 2897:2008 concerning The Testing Method of Microbial Contamination in Meat, Eggs, and Milk and Their Processed Products. The E. coli O157 was confirmed with O157 latex agglutination test and H7 antiserum test. The data were analyzed using a descriptive test and risk factors were analyzed using chi-square and odds ratio. The results showed that E. coli O157:H7 was found in 2 of 3 samples (66,7%) isolated from Kaget Market. The significant risk factors (P<0,005) for the presence of E. coli were traders with age of more 45 years, kiosk distance of less than 5 meters from the highway, and washing hands using unclean water. The presence of E. coli O157:H7 will be a threat for consumer health.
{"title":"Presence of Escherichia coli O157:H7 in Chicken Meat at Traditional Markets in Pangkalpinang City","authors":"Ahmad Nurhakim, D. Lukman, H. Pisestyani","doi":"10.22146/jsv.71162","DOIUrl":"https://doi.org/10.22146/jsv.71162","url":null,"abstract":"This study was aimed to identify the presence of E. coli O157:H7 and analyze the association between factors and frequency of E. coli in chicken meat sold at Kaget Market, Pagi Market, and Pembangunan Market in Pangkalpinang City. A total of 30 chicken meat samples were collected proportionally using simple random sampling. This study was used a cross sectional method. The examination of E. coli was carried out based on SNI 2897:2008 concerning The Testing Method of Microbial Contamination in Meat, Eggs, and Milk and Their Processed Products. The E. coli O157 was confirmed with O157 latex agglutination test and H7 antiserum test. The data were analyzed using a descriptive test and risk factors were analyzed using chi-square and odds ratio. The results showed that E. coli O157:H7 was found in 2 of 3 samples (66,7%) isolated from Kaget Market. The significant risk factors (P<0,005) for the presence of E. coli were traders with age of more 45 years, kiosk distance of less than 5 meters from the highway, and washing hands using unclean water. The presence of E. coli O157:H7 will be a threat for consumer health.","PeriodicalId":17708,"journal":{"name":"Jurnal Sain Veteriner","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2022-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"73500264","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Amalia Sutriana, T. N. Siregar, Feby Claudia Sirait, Juli Melia, Hafizuddin .
Penelitian ini bertujuan mengetahui pengaruh pemberian gonadotropin releasing hormone (GnRH) terhadap peningkatan kualitas spermatozoa sapi bali. Penelitian ini menggunakan dua ekor sapi bali berumur 2 dan 4 tahun dengan rancangan pola bujursangkar latin 2 perlakuan dan 2 periode perlakuan. Perlakuan yang dilakukan adalah P1 (NaCl fisiologis) dan P2 (GnRH 100 µg) dengan interval periode waktu perlakuan adalah satu minggu. Sampel semen dikoleksi menggunakan vagina buatan tiga hari setelah perlakuan. Data hasil pemeriksaan kualitas spermatozoa sapi bali dianalisis dengan uji Wilcoxon Signed Ranks Test. Berdasarkan uji statistik menunjukkan bahwa pemberian GnRH 100 µg tidak berpengaruh nyata (P>0,05) terhadap pemeriksaan warna, pH, konsistensi, volume, konsentrasi dan motilitas spermatozoa sapi bali. Hasil pemeriksaan menunjukkan bahwa warna semen pada kedua perlakuan adalah krem dan putih susu. Derajat keasaman (pH); konsistensi; volume semen (ml); konsentrasi (10⁶/ml); dan motilitas spermatozoa (%) sapi bali pada P1 vs P2 adalah 7,0±0,0 vs 7,0±0,0; 2,5±0,71 vs 3,0±0,0; 4,5±2,8 vs 4,8±2,8; 925±106,07 vs 1210±155,56; dan 69,0±1,41 vs 71,5±0,71 (P>0,05). Disimpulkan bahwa pemberian GnRH 100 µg tidak memengaruhi kualitas spermatozoa sapi bali.
本研究旨在研究影响影响巴厘岛牛的精子质量。这项研究使用了巴厘岛的两头2岁和4岁的奶牛，它们设计了拉丁市场模式2种治疗和2种治疗周期。做法是P1(生理食盐)的待遇和P2 (GnRH 100µg)间隔时间是一周的待遇。治疗三天后，用人造阴道收集了精液样本。巴厘岛牛的精子质量检查数据是用Wilcoxon Signed Ranks测试分析的。根据统计数据显示,给予GnRH测试100µg真的没有影响(P > 0。05)对颜色、pH值、检查的一致性,体积浓度、精子活动力牛巴厘岛。检查结果显示，治疗方法中的精液颜色为米色和乳白色。酸度(pH);一致性;混凝土体积(ml);浓度(10⁶/ ml);和牛的精子活动力(%)巴厘岛的P1与P2是7.0±0,0 vs 7.0±0,0;2.5±0,71 vs 3.0±0,0;4.5±2.8 vs 4.8±2.8;1210 3034±106.07 vs±155.56;和69.0±1.41 vs 71.5±0,71 (P > 0。05)。推断给予GnRH 100µg巴厘牛不会影响精子质量。
{"title":"Pengaruh Pemberian Gonadotropin Releasing Hormone (GnRH) Terhadap Peningkatan Kualitas Spermatozoa Sapi Bali Pengaruh Pemberian Gonadotropin Releasing Hormone (GnRH) Terhadap Peningkatan Kualitas Spermatozoa Sapi Bali","authors":"Amalia Sutriana, T. N. Siregar, Feby Claudia Sirait, Juli Melia, Hafizuddin .","doi":"10.22146/jsv.65067","DOIUrl":"https://doi.org/10.22146/jsv.65067","url":null,"abstract":"Penelitian ini bertujuan mengetahui pengaruh pemberian gonadotropin releasing hormone (GnRH) terhadap peningkatan kualitas spermatozoa sapi bali. Penelitian ini menggunakan dua ekor sapi bali berumur 2 dan 4 tahun dengan rancangan pola bujursangkar latin 2 perlakuan dan 2 periode perlakuan. Perlakuan yang dilakukan adalah P1 (NaCl fisiologis) dan P2 (GnRH 100 µg) dengan interval periode waktu perlakuan adalah satu minggu. Sampel semen dikoleksi menggunakan vagina buatan tiga hari setelah perlakuan. Data hasil pemeriksaan kualitas spermatozoa sapi bali dianalisis dengan uji Wilcoxon Signed Ranks Test. Berdasarkan uji statistik menunjukkan bahwa pemberian GnRH 100 µg tidak berpengaruh nyata (P>0,05) terhadap pemeriksaan warna, pH, konsistensi, volume, konsentrasi dan motilitas spermatozoa sapi bali. Hasil pemeriksaan menunjukkan bahwa warna semen pada kedua perlakuan adalah krem dan putih susu. Derajat keasaman (pH); konsistensi; volume semen (ml); konsentrasi (10⁶/ml); dan motilitas spermatozoa (%) sapi bali pada P1 vs P2 adalah 7,0±0,0 vs 7,0±0,0; 2,5±0,71 vs 3,0±0,0; 4,5±2,8 vs 4,8±2,8; 925±106,07 vs 1210±155,56; dan 69,0±1,41 vs 71,5±0,71 (P>0,05). Disimpulkan bahwa pemberian GnRH 100 µg tidak memengaruhi kualitas spermatozoa sapi bali.","PeriodicalId":17708,"journal":{"name":"Jurnal Sain Veteriner","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2022-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"87882123","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Sarmin Sarmin, I. Widiyono, Devita Anggraeni, C. M. Airin, P. Astuti
This study examines the prolactin hormone profile in Etaawa, Sapera and Saanen lactating goats, adult females, pregnant does, and yearlings. This study used a total of 80 randomly selected goats with details of 26 Ettawa Crossbreed goats (eight lactating, six adult females, six pregnant, and six heifers), 32 Sapera goats (eight lactating, six adult females, twelve pregnant, and six yearlings) and 22 Saanen goats (five lactating, four adult females, six pregnant and yearling). All goats were declared clinically healthy. Blood sampling was performed through the jugular vein in each animal; then, serum was analyzed with an Enzyme-linked immunosorbent assay (ELISA) to prolactin concentration. High prolactin concentrations were found in Saanen goats in pregnant physiological status compared to Sapera and Ettawa crossbreed goats.
{"title":"Kadar Hormon Prolaktin Pada Kambing Peranakan Ettawa,Sapera dan Saanen Pada Berbagai Status Fisiologi","authors":"Sarmin Sarmin, I. Widiyono, Devita Anggraeni, C. M. Airin, P. Astuti","doi":"10.22146/jsv.76207","DOIUrl":"https://doi.org/10.22146/jsv.76207","url":null,"abstract":" This study examines the prolactin hormone profile in Etaawa, Sapera and Saanen lactating goats, adult females, pregnant does, and yearlings. This study used a total of 80 randomly selected goats with details of 26 Ettawa Crossbreed goats (eight lactating, six adult females, six pregnant, and six heifers), 32 Sapera goats (eight lactating, six adult females, twelve pregnant, and six yearlings) and 22 Saanen goats (five lactating, four adult females, six pregnant and yearling). All goats were declared clinically healthy. Blood sampling was performed through the jugular vein in each animal; then, serum was analyzed with an Enzyme-linked immunosorbent assay (ELISA) to prolactin concentration. High prolactin concentrations were found in Saanen goats in pregnant physiological status compared to Sapera and Ettawa crossbreed goats. ","PeriodicalId":17708,"journal":{"name":"Jurnal Sain Veteriner","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2022-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"81781066","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
PREVALENCE OF AVIAN PATHOGENIC Escherichia coli (APEC) STRAINS ON COLIBACILLOSIS IN QUAIL Wahyu Prihtiyantoro1), Khusnan1), Mitra Slipranata2)† dan Imron Rosyidi2) 1 Akademi Peternakan Brahmaputra, Jl. Ki Ageng Pemanahan, Nitikan, Sorosutan Umbulharjo Yogyakarta,Tlp. 0274-384370, E-mail: wahyumatra@gmail.com 2 Fakultas Kedokteran Hewan, Universitas Gadjah Mada, Jl. Fauna 2, Karangmalang, Yogyakarta 55281 ABSTRACTAvian Pathogenic Escherichia coli (APEC) is a pathogen that causes colibacillosis in poultry, including salpingitis, omphalitis, cellulitis, swollen head syndrome, coligranuloma yolk sac inflammation, and air sacs inflammation. APEC is a zoonotic strain which spread through raw meat and processed meat products of animals and birds. In this research, the isolation and the identification of Escherichia coli were done by using selective media MacConkey, Kligger Iron Agar, and Gram staining. To analyse genetopically, polymerase chain reaction (PCR) was used to detect 16SrRNA genes, vt1 genes, and vt2 genes. Thirty one (55,36%) isolates of 56 specimens collected from quail were detected as Escherichia coli. The detection of APEC strains towards 31 Escherichia coli isolates were done by using polymerase chain reaction (PCR) with vt1 and vt2 specific primer. From the results showed that 32,26% (10/31) was APEC strains and 67.74% was non-APEC strains. From 10 isolates, 90% had vt1 gene and 10% had vt2 gene. Escherichia coli isolates were found in eyes (32,26%), infraorbital sinus fluid (32,26%), nasal fluid (16,20%), also in lungs, air sacs, ascites, and heart for 3,2% each. The isolates could not be found in the specimens from the skull. These isolates are zoonotic and have an impact on human health.Keywords: APEC, colibacillosis, quail, vt1 and vt2
{"title":"Prevalensi Strain Avian Pathogenik Escherichia coli (APEC) Penyebab Kolibasilosis pada Burung Puyuh","authors":"Wahyu Prihtiyantoro Sarwohadi","doi":"10.22146/jsv.23358","DOIUrl":"https://doi.org/10.22146/jsv.23358","url":null,"abstract":"PREVALENCE OF AVIAN PATHOGENIC Escherichia coli (APEC) STRAINS ON COLIBACILLOSIS IN QUAIL Wahyu Prihtiyantoro1), Khusnan1), Mitra Slipranata2)† dan Imron Rosyidi2) 1 Akademi Peternakan Brahmaputra, Jl. Ki Ageng Pemanahan, Nitikan, Sorosutan Umbulharjo Yogyakarta,Tlp. 0274-384370, E-mail: wahyumatra@gmail.com 2 Fakultas Kedokteran Hewan, Universitas Gadjah Mada, Jl. Fauna 2, Karangmalang, Yogyakarta 55281 ABSTRACTAvian Pathogenic Escherichia coli (APEC) is a pathogen that causes colibacillosis in poultry, including salpingitis, omphalitis, cellulitis, swollen head syndrome, coligranuloma yolk sac inflammation, and air sacs inflammation. APEC is a zoonotic strain which spread through raw meat and processed meat products of animals and birds. In this research, the isolation and the identification of Escherichia coli were done by using selective media MacConkey, Kligger Iron Agar, and Gram staining. To analyse genetopically, polymerase chain reaction (PCR) was used to detect 16SrRNA genes, vt1 genes, and vt2 genes. Thirty one (55,36%) isolates of 56 specimens collected from quail were detected as Escherichia coli. The detection of APEC strains towards 31 Escherichia coli isolates were done by using polymerase chain reaction (PCR) with vt1 and vt2 specific primer. From the results showed that 32,26% (10/31) was APEC strains and 67.74% was non-APEC strains. From 10 isolates, 90% had vt1 gene and 10% had vt2 gene. Escherichia coli isolates were found in eyes (32,26%), infraorbital sinus fluid (32,26%), nasal fluid (16,20%), also in lungs, air sacs, ascites, and heart for 3,2% each. The isolates could not be found in the specimens from the skull. These isolates are zoonotic and have an impact on human health.Keywords: APEC, colibacillosis, quail, vt1 and vt2","PeriodicalId":17708,"journal":{"name":"Jurnal Sain Veteriner","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2022-11-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"86605043","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
H. Pisestyani, Irena Ivania, Ardilasunu Wicaksono, D. Lukman, M. Sudarwanto
Milk products consumption has become a new trend of healthy lifestyle among students in order to meet appropriate daily nutrients. This study was aimed to measure the total number of microbes found in milk drinks sold around the IPB Dramaga and Cilibende and to determine factors that influence it. Samples were collected from 13 milk vendors within radius of 2 kilometers from outermost point of campus and taken with 3 repetitions. Calculation of the total number of microbes was done by using the plate count method in accordance with SNI 2897-2008 about testing method of microbial contamination in meat, eggs, and milk, as well as processed products and SNI 01-6366-2000 about the maximum limit of microbial contamination in food. Risk factors’ data was taken by interviewing milk drinks seller using structured questionnaire. Factors that influenced microbial contamination were analyzed using Chi-Square test. The results showed that 74.4% of milk drinks samples had a high total number of microbes. The average total number of microbes in Dramaga and Cilibende samples was 1.0 x 105 ± 1.4 x 105 cfu / ml and 1.1 x 107 ± 1.2 x 107 cfu/ml. The total number of microbes are significantly influenced (p<0.05) by equipments used, the cleanliness of the equipment, the cleanliness of the table, washing hands practice, and the distance from the crowd. The high number of microbial contamination in milk drinks could damage the quality of milk and shorten the shelf life.
{"title":"Microbiological Quality of Milk Flavored Ready to Drink and Hygiene Practice of Street Vendors","authors":"H. Pisestyani, Irena Ivania, Ardilasunu Wicaksono, D. Lukman, M. Sudarwanto","doi":"10.22146/jsv.60996","DOIUrl":"https://doi.org/10.22146/jsv.60996","url":null,"abstract":"Milk products consumption has become a new trend of healthy lifestyle among students in order to meet appropriate daily nutrients. This study was aimed to measure the total number of microbes found in milk drinks sold around the IPB Dramaga and Cilibende and to determine factors that influence it. Samples were collected from 13 milk vendors within radius of 2 kilometers from outermost point of campus and taken with 3 repetitions. Calculation of the total number of microbes was done by using the plate count method in accordance with SNI 2897-2008 about testing method of microbial contamination in meat, eggs, and milk, as well as processed products and SNI 01-6366-2000 about the maximum limit of microbial contamination in food. Risk factors’ data was taken by interviewing milk drinks seller using structured questionnaire. Factors that influenced microbial contamination were analyzed using Chi-Square test. The results showed that 74.4% of milk drinks samples had a high total number of microbes. The average total number of microbes in Dramaga and Cilibende samples was 1.0 x 105 ± 1.4 x 105 cfu / ml and 1.1 x 107 ± 1.2 x 107 cfu/ml. The total number of microbes are significantly influenced (p<0.05) by equipments used, the cleanliness of the equipment, the cleanliness of the table, washing hands practice, and the distance from the crowd. The high number of microbial contamination in milk drinks could damage the quality of milk and shorten the shelf life.","PeriodicalId":17708,"journal":{"name":"Jurnal Sain Veteriner","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2022-08-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"83023565","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
M. Z. Ritonga, T. Siregar, M. Hanafiah, Muttaqien -, Elvina Andali Putri
Back leg paresis is muscle weakness that can cause the inability to walk or support the back of the body. A domestic cat, female, black tabby hair colour with a bodyweight of 2.9 kg has weakness in both hind legs so it cannot lift the back of the body properly but there is still a reflex when pressing using tweezers sirurgis on the gluteus muscle tendon. The flexor reflexes on the digits are still present but slightly. From the physical examination, it was concluded that there was a disturbance in the segment IV spinal cord, precisely at L7-S1. Cats also experience problems with defecation, namely the patient's stool is watery inconsistency. The results of the X-Ray examination showed that the segments of the os. lumbar, os. femur, os. tibia, os. fibula, os. metatarsal to digit there are no fractures and also the visceral organs do not experience changes and abnormalities. However, there is a bias picture, namely compression on the 7th segment (S4-L5, L6 and L7). The diagnosis was that the cat had disorders of the spinal cord segments IV (L4-S2) and V, to be precise, from L7 to S2. Electroacupuncture therapy at points BL-18, BL-19, SP-6, ST-36 and LIV-3 was carried out for three weeks, namely twice in the first week and once in the second and third weeks. Oral drug therapy in the form of Neurobion® tablets is taken orally during therapy.
{"title":"Combination of Acupuncture Points in The Treatment of Hind Leg Paresis with Electroacupuncture Methods in Domestic Cats","authors":"M. Z. Ritonga, T. Siregar, M. Hanafiah, Muttaqien -, Elvina Andali Putri","doi":"10.22146/jsv.67650","DOIUrl":"https://doi.org/10.22146/jsv.67650","url":null,"abstract":"Back leg paresis is muscle weakness that can cause the inability to walk or support the back of the body. A domestic cat, female, black tabby hair colour with a bodyweight of 2.9 kg has weakness in both hind legs so it cannot lift the back of the body properly but there is still a reflex when pressing using tweezers sirurgis on the gluteus muscle tendon. The flexor reflexes on the digits are still present but slightly. From the physical examination, it was concluded that there was a disturbance in the segment IV spinal cord, precisely at L7-S1. Cats also experience problems with defecation, namely the patient's stool is watery inconsistency. The results of the X-Ray examination showed that the segments of the os. lumbar, os. femur, os. tibia, os. fibula, os. metatarsal to digit there are no fractures and also the visceral organs do not experience changes and abnormalities. However, there is a bias picture, namely compression on the 7th segment (S4-L5, L6 and L7). The diagnosis was that the cat had disorders of the spinal cord segments IV (L4-S2) and V, to be precise, from L7 to S2. Electroacupuncture therapy at points BL-18, BL-19, SP-6, ST-36 and LIV-3 was carried out for three weeks, namely twice in the first week and once in the second and third weeks. Oral drug therapy in the form of Neurobion® tablets is taken orally during therapy.","PeriodicalId":17708,"journal":{"name":"Jurnal Sain Veteriner","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2022-08-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"74395704","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pasha Glabella, Salma Ramandhanti Putri, Ery Haryani, A. Wahyuni
Biji jintan hitam (Nigella sativa L.) mengandung senyawa aktif seperti thymoquinone, carvacrol, dan thymol yang berperan sebagai antijamur. Penelitian ini bertujuan untuk mengetahui kemampuan ekstrak biji jintan hitam dalam menghambat pertumbuhan jamur Microsporum gypseum yang merupakan salah satu agen penyebab dermatitis pada anjing. Kemampuan ekstrak biji jintan hitam dalam menghambat pertumbuhan jamur Mircrosporum gypseum diuji dengan metode difusi agar dengan teknik sumuran (Agar Well Diffusion). Rancangan penelitian ini menggunakan Rancangan Acak Lengkap (RAL) dengan 7 perlakuan konsentrasi ekstrak biji jintan hitam terhadap pertumbuhan jamur Microsporum gypseum yaitu 10%, 25%, 50%, 75%, 100%, flukonazol digunakan sebagai kontrol positif dan phospat buffer saline (PBS) kontrol negatif. Hasil penelitian menunjukkan bahwa ekstrak biji jintan hitam dengan konsentrasi mulai dari 25%, 50%, 75% dan 100% dapat menghambat pertumbuhan jamur dengan rerata lebar zona hambat secara berurutan ± 3,67 mm., ± 2,84 mm., ± 3,67 mm., dan ± 4,00 mm. Ekstrak biji jintan hitam mampu menghambat pertumbuhan Microsporum gypseum. Perlu penelitian lebih lanjut untuk mendapatkan hasil optimal sehingga berpeluang sebagai anti fungal pada penyakit dermatitis pada anjing.
{"title":"Uji In Vitro Efektivitas Ekstrak Biji Jintan Hitam (Nigella Sativa L.) terhadap Pertumbuhan Microsporum gypseum Penyebab Dermatitis pada Anjing","authors":"Pasha Glabella, Salma Ramandhanti Putri, Ery Haryani, A. Wahyuni","doi":"10.22146/jsv.69251","DOIUrl":"https://doi.org/10.22146/jsv.69251","url":null,"abstract":"Biji jintan hitam (Nigella sativa L.) mengandung senyawa aktif seperti thymoquinone, carvacrol, dan thymol yang berperan sebagai antijamur. Penelitian ini bertujuan untuk mengetahui kemampuan ekstrak biji jintan hitam dalam menghambat pertumbuhan jamur Microsporum gypseum yang merupakan salah satu agen penyebab dermatitis pada anjing. Kemampuan ekstrak biji jintan hitam dalam menghambat pertumbuhan jamur Mircrosporum gypseum diuji dengan metode difusi agar dengan teknik sumuran (Agar Well Diffusion). Rancangan penelitian ini menggunakan Rancangan Acak Lengkap (RAL) dengan 7 perlakuan konsentrasi ekstrak biji jintan hitam terhadap pertumbuhan jamur Microsporum gypseum yaitu 10%, 25%, 50%, 75%, 100%, flukonazol digunakan sebagai kontrol positif dan phospat buffer saline (PBS) kontrol negatif. Hasil penelitian menunjukkan bahwa ekstrak biji jintan hitam dengan konsentrasi mulai dari 25%, 50%, 75% dan 100% dapat menghambat pertumbuhan jamur dengan rerata lebar zona hambat secara berurutan ± 3,67 mm., ± 2,84 mm., ± 3,67 mm., dan ± 4,00 mm. Ekstrak biji jintan hitam mampu menghambat pertumbuhan Microsporum gypseum. Perlu penelitian lebih lanjut untuk mendapatkan hasil optimal sehingga berpeluang sebagai anti fungal pada penyakit dermatitis pada anjing.","PeriodicalId":17708,"journal":{"name":"Jurnal Sain Veteriner","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2022-08-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"88148293","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Diza Raudya Tuzzahra, E. Ariyanto, Septiyani -, A. Rosdianto
There is a transition period that dairy cattle always pass through in their reproduction cycle, making them more stressed. Stress can simply be identified by the ratio of neutrophil (N) and lymphocyte (L) at the haematologic state of dairy cattle. The purpose of this study is to evaluate stress conditions in dairy cattle’s transition period based on neutrophil and lymphocyte ratio. Twenty samples of dairy cattle’s blood in prepartum and postpartum condition are being analyzed with a hematology analyzer. The result of this study shows that the average of neutrophil has increased insignificantly from prepartum to postpartum period as 4.14 103/µL ± 1.51 to 4.27 103/µL ± 2.08 or 1.03% with a normal interval of neutrophil is 1.7-6.0 103/µL. However, the average of lymphocytes decreased insignificantly from 3.23 103/µL ± 1.13 to 3.03 103/µL ± 1.23 or 0.93% with a normal interval of lymphocyte is 1.8-8.1 103/µL. Then, the average of neutrophil and lymphocyte ratio has increased insignificantly from 1.35 103/µL ± 0.46 to 1.47 103/µL ± 0.60 or 1.09%. Based on this result, the ratio of neutrophils per lymphocyte still in the normal range. In the transition period, only a few dairy cattle have a stressful condition. The stress condition in dairy cattle can be identified by increasing neutrophil (neutrophilia) and reducing lymphocyte (lymphocytopenia).
{"title":"Comparative of Neutrophils and Lymphocyte Ratio for Dairy Cattle in Prepartum and Postpartum Condition","authors":"Diza Raudya Tuzzahra, E. Ariyanto, Septiyani -, A. Rosdianto","doi":"10.22146/jsv.67404","DOIUrl":"https://doi.org/10.22146/jsv.67404","url":null,"abstract":"There is a transition period that dairy cattle always pass through in their reproduction cycle, making them more stressed. Stress can simply be identified by the ratio of neutrophil (N) and lymphocyte (L) at the haematologic state of dairy cattle. The purpose of this study is to evaluate stress conditions in dairy cattle’s transition period based on neutrophil and lymphocyte ratio. Twenty samples of dairy cattle’s blood in prepartum and postpartum condition are being analyzed with a hematology analyzer. The result of this study shows that the average of neutrophil has increased insignificantly from prepartum to postpartum period as 4.14 103/µL ± 1.51 to 4.27 103/µL ± 2.08 or 1.03% with a normal interval of neutrophil is 1.7-6.0 103/µL. However, the average of lymphocytes decreased insignificantly from 3.23 103/µL ± 1.13 to 3.03 103/µL ± 1.23 or 0.93% with a normal interval of lymphocyte is 1.8-8.1 103/µL. Then, the average of neutrophil and lymphocyte ratio has increased insignificantly from 1.35 103/µL ± 0.46 to 1.47 103/µL ± 0.60 or 1.09%. Based on this result, the ratio of neutrophils per lymphocyte still in the normal range. In the transition period, only a few dairy cattle have a stressful condition. The stress condition in dairy cattle can be identified by increasing neutrophil (neutrophilia) and reducing lymphocyte (lymphocytopenia).","PeriodicalId":17708,"journal":{"name":"Jurnal Sain Veteriner","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2022-08-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"83856152","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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