Fauziah Fitriana, Riza Resita, Y. Disastra, Gioknio Happy Alfatik, C. A. Artdita, Ari Haryanto, Fatkhanuddin Aziz
Penentuan jenis kelamin pada beberapa spesies burung cukup sulit dilakukan dikarenakan jantan dan betina memiliki ciri morfologi yang sama (monomorfik), salah satunya famili Columbidae. Teknik penentuan jenis kelamin burung secara molekuler yang populer adalah metode Polymerase Chain Reaction (PCR) dengan gen target Chromo Helicase DNA-binding (CHD), namun keberhasilan amplifikasi gen target pada PCR tersebut dipengaruhi salah satunya kesesuaian DNA template dengan primer yang digunakan. Penelitian ini bertujuan untuk mengevaluasi 5 jenis primer PCR P2/P8, 2550F/2718R, CHD1F/CHD1R, 1237L/1272H dan CHD1LF/CHD1LR untuk menentukan jenis kelamin famili Columbidae. Penelitian ini dilakukan dengan menguji 5 jenis primer tersebut di atas pada sampel DNA tiap pasang jantan dan betina dari burung merpati, balam jambi, punai, derkuku, dan perkutut. Hasil penelitian diketahui primer CHD1LF/CHD1LR menunjukkan hasil terbaik dan direkomendasikan untuk menentukan jenis kelamin famili Columbidae.
{"title":"Komparasi Lima Jenis Primer Polymerase Chain Reaction Untuk Mengidentifikasi Kelamin Burung Famili Columbidae Yang Akurat","authors":"Fauziah Fitriana, Riza Resita, Y. Disastra, Gioknio Happy Alfatik, C. A. Artdita, Ari Haryanto, Fatkhanuddin Aziz","doi":"10.22146/jsv.68787","DOIUrl":"https://doi.org/10.22146/jsv.68787","url":null,"abstract":"Penentuan jenis kelamin pada beberapa spesies burung cukup sulit dilakukan dikarenakan jantan dan betina memiliki ciri morfologi yang sama (monomorfik), salah satunya famili Columbidae. Teknik penentuan jenis kelamin burung secara molekuler yang populer adalah metode Polymerase Chain Reaction (PCR) dengan gen target Chromo Helicase DNA-binding (CHD), namun keberhasilan amplifikasi gen target pada PCR tersebut dipengaruhi salah satunya kesesuaian DNA template dengan primer yang digunakan. Penelitian ini bertujuan untuk mengevaluasi 5 jenis primer PCR P2/P8, 2550F/2718R, CHD1F/CHD1R, 1237L/1272H dan CHD1LF/CHD1LR untuk menentukan jenis kelamin famili Columbidae. Penelitian ini dilakukan dengan menguji 5 jenis primer tersebut di atas pada sampel DNA tiap pasang jantan dan betina dari burung merpati, balam jambi, punai, derkuku, dan perkutut. Hasil penelitian diketahui primer CHD1LF/CHD1LR menunjukkan hasil terbaik dan direkomendasikan untuk menentukan jenis kelamin famili Columbidae.","PeriodicalId":17708,"journal":{"name":"Jurnal Sain Veteriner","volume":"7 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-08-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"85588769","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Desy Cahya Widianingrum, Satrio Adi Prakoso, Mila Riskiatul Rohma, Muhammad Faza Hunafah, Muhammad Iqbal, Dhimas Yusantoro
Agen penyebab penyakit Chronic Respiratory Disease (CRD) berasal dari bakteri Mycoplasma gallisepticum yang menginfeksi saluran pernapasan pada ternak unggas seperti ayam, itik, angsa, entok, kalkun, burung dara, dan lain-lain. Faktor yang dapat memperburuk terjadinya infeksi penyakit ini yakni umur ternak, jenis kelamin, stres, dan lingkungan. Infeksi bakteri ini lebih rentan pada ayam yang berumur muda dan ayam betina dibandingkan dengan ayam dewasa dan ayam jantan. Gejala klinis dari penyakit ini yaitu terdengarnya suara ngorok pada ayam di malam hari, keluarnya catarrhal dari rongga hidung, batuk, radang conjunctiva, dan bersin. Masa penyakit CRD berkisar antara 4 - 21 hari dan mudah menular. Metode uji laboratrium untuk mengidentifikasi pemeriksaan terhadap kontaminasi bakteri Mycoplasma gallispeticum di kandang diantarnya uji seroligi seperti HI (Hemaglutination Inhibition Test), RSA (Rapid Serum Aglutination Test), maupun ELISA (Enzyme Linked Immuno Sorbent Assay). Kejadian penyakit CRD hingga saat ini yang masih ditemukan di seluruh wilayah Indonesia menyebabkan kerugian bagi peternak. Kerugian ekonomis akibat penyakit ini dapat diminimalisir dengan pengetahuan peternak akan pentingnya program biosekuriti yang harus diterapkan secara konsisten, serta peningkatan imunitas ternak seperti vaksinasi serta penggunaan antimikroba yang aman dan tidak menimbulkan residu.
{"title":"Penyakit Chronic Respiratory Disease (CRD) : Etiologi, Epidemiologi, Patogenesis, Gejala Klinis, Diagnosis, Pengobatan dan Pencegahan","authors":"Desy Cahya Widianingrum, Satrio Adi Prakoso, Mila Riskiatul Rohma, Muhammad Faza Hunafah, Muhammad Iqbal, Dhimas Yusantoro","doi":"10.22146/jsv.56683","DOIUrl":"https://doi.org/10.22146/jsv.56683","url":null,"abstract":"Agen penyebab penyakit Chronic Respiratory Disease (CRD) berasal dari bakteri Mycoplasma gallisepticum yang menginfeksi saluran pernapasan pada ternak unggas seperti ayam, itik, angsa, entok, kalkun, burung dara, dan lain-lain. Faktor yang dapat memperburuk terjadinya infeksi penyakit ini yakni umur ternak, jenis kelamin, stres, dan lingkungan. Infeksi bakteri ini lebih rentan pada ayam yang berumur muda dan ayam betina dibandingkan dengan ayam dewasa dan ayam jantan. Gejala klinis dari penyakit ini yaitu terdengarnya suara ngorok pada ayam di malam hari, keluarnya catarrhal dari rongga hidung, batuk, radang conjunctiva, dan bersin. Masa penyakit CRD berkisar antara 4 - 21 hari dan mudah menular. Metode uji laboratrium untuk mengidentifikasi pemeriksaan terhadap kontaminasi bakteri Mycoplasma gallispeticum di kandang diantarnya uji seroligi seperti HI (Hemaglutination Inhibition Test), RSA (Rapid Serum Aglutination Test), maupun ELISA (Enzyme Linked Immuno Sorbent Assay). Kejadian penyakit CRD hingga saat ini yang masih ditemukan di seluruh wilayah Indonesia menyebabkan kerugian bagi peternak. Kerugian ekonomis akibat penyakit ini dapat diminimalisir dengan pengetahuan peternak akan pentingnya program biosekuriti yang harus diterapkan secara konsisten, serta peningkatan imunitas ternak seperti vaksinasi serta penggunaan antimikroba yang aman dan tidak menimbulkan residu. ","PeriodicalId":17708,"journal":{"name":"Jurnal Sain Veteriner","volume":"22 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-08-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"76628341","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
N. Hasanah, P. E. Sudaryatma, Imanuddin Razaq, Ni Nyoman Eriawati, W. Nugraha, Hidayati Kumalasari, Ni Putu Arya Shintya Anggraeni, Ida Ayu Mirah Meliana Dewi
The fisheries sector provided a significant contribution to the Indonesian economy by increasing export activities in every year. The exported fisheries product are categorized of live fish, frozen fish, preservation products from various types of fish, crustaceans, and molluscs. The contamination of pathogenic bacteria Vibrio parahaemolyticus and Escherichia coli causing healthy problems originating from the fishery sector (sea-food borne disease). These two bacteria contaminated fisheries product is due to mishandling and storaging in the processing, which causes acute diarrhea, gastrointestinal infections and fever. The multiplex polymerase chain reaction (mPCR) method was developed to increase the efficiency of time, effort and accuracy of the bacterial contamination testing process. The mPCR method begins with the optimization of the two bacterial gene targets, sensitivity test, specificity test and then applied to samples of fishery products. The mPCR method is carried out in two mechanisms, namely “one-run” conducted from bacterial colonies isolated on agar media and “one-tube” which is applied directly from fishery products. The results of the development of the mPCR method on V. parahaemolyticus and E. coli resulted in sensitivity at concentrations of DNA 5.6 pg/ml and DNA 5.5 pg/ml, respectively. One-tube mPCR application obtained 7 positive colonies of V. parahaemolyticus and 38 positive colonies of E. coli. Meanwhile, one-tube mPCR which was applied directly from shrimp samples could identify the two bacteria.
{"title":"Early Detection of Contamination Vibrio parahaemolyticus and Escherichia coli in Fisheries Product Using Multiplex Polymerase Chain Reaction","authors":"N. Hasanah, P. E. Sudaryatma, Imanuddin Razaq, Ni Nyoman Eriawati, W. Nugraha, Hidayati Kumalasari, Ni Putu Arya Shintya Anggraeni, Ida Ayu Mirah Meliana Dewi","doi":"10.22146/jsv.73314","DOIUrl":"https://doi.org/10.22146/jsv.73314","url":null,"abstract":"The fisheries sector provided a significant contribution to the Indonesian economy by increasing export activities in every year. The exported fisheries product are categorized of live fish, frozen fish, preservation products from various types of fish, crustaceans, and molluscs. The contamination of pathogenic bacteria Vibrio parahaemolyticus and Escherichia coli causing healthy problems originating from the fishery sector (sea-food borne disease). These two bacteria contaminated fisheries product is due to mishandling and storaging in the processing, which causes acute diarrhea, gastrointestinal infections and fever. The multiplex polymerase chain reaction (mPCR) method was developed to increase the efficiency of time, effort and accuracy of the bacterial contamination testing process. The mPCR method begins with the optimization of the two bacterial gene targets, sensitivity test, specificity test and then applied to samples of fishery products. The mPCR method is carried out in two mechanisms, namely “one-run” conducted from bacterial colonies isolated on agar media and “one-tube” which is applied directly from fishery products. The results of the development of the mPCR method on V. parahaemolyticus and E. coli resulted in sensitivity at concentrations of DNA 5.6 pg/ml and DNA 5.5 pg/ml, respectively. One-tube mPCR application obtained 7 positive colonies of V. parahaemolyticus and 38 positive colonies of E. coli. Meanwhile, one-tube mPCR which was applied directly from shrimp samples could identify the two bacteria.","PeriodicalId":17708,"journal":{"name":"Jurnal Sain Veteriner","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-08-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"86225099","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
A. P. Cahyani, Budi Purwo Widiarso, Supriyanto -, Wida Wahidah Mubarokah
Haemonchosis is a gastrointestinal parasitic disease in sheep caused by Haemonchus contortus. The biggest economic losses due to this disease are mortality, decreased production, stunted growth, and low body weight. The existence of resistance to synthetic anthelmintics causes the development of research on alternative treatments to herbal anthelmintics to be a strategic step. This study aims to analyze the effect of Areca catechu on Haemonchus contortus in vitro so that the Lethal Concentration 50 (LC 50) can be determined. This study was divided into 9 groups. Group I was treated with 2.5% A. catechu crude aqueous extract; group II was treated with 5% of A. catechu crude aqueous extract; group III was treated with 7.5% A. catechu crude aqueous extract; group IV was treated with 10% of A. catechu crude aqueous extract; group V was treated with 12.5% A. catechu crude aqueous extract; group VI was treated with 15% A. catechu crude aqueous extract; Group VII was treated with 17.5% A. catechu crude aqueous extract, group VIII was a negative control (0.9% NaCl) and group IX was a positive control (Albendazole). The mortality of H. contortus was recorded every hour until the worm mortality was 100%. The results were then analyzed using the Reed and Muench method. Based on the in vitro test of Areca catechu crude aqueous extract against H.contortus it can cause mortality in worms with an Lethal Concentration 50 (LC 50) calculation result of 11.11%.
{"title":"In Vitro Anthelmintic Areca catechu Crude Aqueous Extract Against Haemonchus contortus in The Sheep","authors":"A. P. Cahyani, Budi Purwo Widiarso, Supriyanto -, Wida Wahidah Mubarokah","doi":"10.22146/jsv.70399","DOIUrl":"https://doi.org/10.22146/jsv.70399","url":null,"abstract":"Haemonchosis is a gastrointestinal parasitic disease in sheep caused by Haemonchus contortus. The biggest economic losses due to this disease are mortality, decreased production, stunted growth, and low body weight. The existence of resistance to synthetic anthelmintics causes the development of research on alternative treatments to herbal anthelmintics to be a strategic step. This study aims to analyze the effect of Areca catechu on Haemonchus contortus in vitro so that the Lethal Concentration 50 (LC 50) can be determined. This study was divided into 9 groups. Group I was treated with 2.5% A. catechu crude aqueous extract; group II was treated with 5% of A. catechu crude aqueous extract; group III was treated with 7.5% A. catechu crude aqueous extract; group IV was treated with 10% of A. catechu crude aqueous extract; group V was treated with 12.5% A. catechu crude aqueous extract; group VI was treated with 15% A. catechu crude aqueous extract; Group VII was treated with 17.5% A. catechu crude aqueous extract, group VIII was a negative control (0.9% NaCl) and group IX was a positive control (Albendazole). The mortality of H. contortus was recorded every hour until the worm mortality was 100%. The results were then analyzed using the Reed and Muench method. Based on the in vitro test of Areca catechu crude aqueous extract against H.contortus it can cause mortality in worms with an Lethal Concentration 50 (LC 50) calculation result of 11.11%.","PeriodicalId":17708,"journal":{"name":"Jurnal Sain Veteriner","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-08-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"85581914","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Hilma Halimatus Sadiah, Adi Imam Cahyadi, S. Windria
AbstractBacterial infection is one of the biggest health issues in animals and humans. Antibiotic is commonly used in bacterial infection therapy, yet antibiotic misuse can lead into bacterial antibiotic resistance. This means antibiotic is less effective in therapy of resistant bacteria. Green betel leaf has been known as traditional herbal medicine with rich essential oil and consists of phenol with several derivatives that have antibacterial activity. The results of the research that have been carried out can be reported that green betel leaf has the potential to be antibacterial. This herbal can be used as alternative therapy because it is easy to get and have low-level toxicity. In this study we try to discuss about green betel leaf (Piper betle L) as antibacterial with activity against Gram-positive and Gram-negative bacteria by analyzing the component of the active compound and mechanism of action.keywords: antibacterial activity; green betel leaf; Piper betle L AbstrakInfeksi bakteri menjadi masalah yang sudah sering terjadi dan berdampak buruk bagi kesehatan manusia dan hewan. Pengobatan untuk penyakit infeksi biasanya menggunakan antibiotik, namun banyak penggunaan antibiotik yang disalahgunakan sehingga dapat mengakibatkan terjadinya resistensi dan bakteri menjadi tidak sensitif terhadap pengobatan antibiotik. Daun sirih hijau merupakan salah satu obat tradisional yang memiliki kandungan minyak atsiri dan komponen utamanya terdiri fenol dengan beberapa turunannya yang berperan sebagai antibakteri. Hasil dari penelitian yang telah dilakukan dapat dinyatakan bahwa daun sirih hijau dapat berpotensi sebagai antibakteri. Selain itu daun sirih hijau mudah didapat dan memiliki toksisitas rendah yang menyebabkan tanaman tradisional tersebut dijadikan sebagai alternatif dalam mengobati infeksi bakteri. Studi literatur ini membahas daun sirih hijau (Piper betle L) sebagai antibakteri terhadap bakteri Gram positif dan Gram negatif meliputi komponen senyawa aktif dan mekanismenya.Kata kunci : aktivitas antibakteri; daun sirih hijau; Piper betle L
摘要细菌感染是动物和人类最大的健康问题之一。抗生素是细菌感染治疗中常用的抗生素，但抗生素的滥用会导致细菌产生耐药性。这意味着抗生素对耐药细菌的治疗效果较差。槟榔叶是一种传统的中草药，含有丰富的精油，由苯酚和几种具有抗菌活性的衍生物组成。已经开展的研究结果表明，槟榔叶具有抗菌的潜力。这种草药可以作为替代疗法，因为它很容易获得，而且毒性很低。本文通过分析槟榔叶的活性成分及其作用机制，探讨槟榔叶对革兰氏阳性菌和革兰氏阴性菌具有抗菌作用。关键词:抗菌活性;槟榔叶;[摘要][中文摘要][font =宋体][font =宋体][font =宋体][font =宋体][font =宋体]Pengobatan为她penyakit infeksi biasanya menggunakan antibiotik, namun banyak penggunaan antibiotik杨disalahgunakan sehingga dapat mengakibatkan terjadinya resistensi丹bakteri menjadi有些sensitif terhadap Pengobatan antibiotik。在传统的杨氏菌群中，有一种叫“杨氏菌群”的菌群，这种菌群通常被称为“杨氏菌群”。Hasil dari penelitian yang telah dilakukan dapat dinyatakan bahwa dauh hijau dapat berpoteni sebagai antibakteri。Selain itu dauh hijau mudah didapat dan memiliki toksisitas rendah yang menyebabkan tanaman传统的tersebut dijadikan sebagai alternatif dalam mengobati infeksi bakteri。研究文献中有学者报道了sirih hijau (Piper betle L)的抗革兰氏菌、抗革兰氏菌、抗革兰氏菌、抗革兰氏菌、抗革兰氏菌、抗革兰氏菌等。Kata kunci:活性菌;dawn sirih hijau;吹笛手
{"title":"Kajian Daun Sirih Hijau (Piper betle L) Sebagai Antibakteri","authors":"Hilma Halimatus Sadiah, Adi Imam Cahyadi, S. Windria","doi":"10.22146/jsv.58745","DOIUrl":"https://doi.org/10.22146/jsv.58745","url":null,"abstract":"AbstractBacterial infection is one of the biggest health issues in animals and humans. Antibiotic is commonly used in bacterial infection therapy, yet antibiotic misuse can lead into bacterial antibiotic resistance. This means antibiotic is less effective in therapy of resistant bacteria. Green betel leaf has been known as traditional herbal medicine with rich essential oil and consists of phenol with several derivatives that have antibacterial activity. The results of the research that have been carried out can be reported that green betel leaf has the potential to be antibacterial. This herbal can be used as alternative therapy because it is easy to get and have low-level toxicity. In this study we try to discuss about green betel leaf (Piper betle L) as antibacterial with activity against Gram-positive and Gram-negative bacteria by analyzing the component of the active compound and mechanism of action.keywords: antibacterial activity; green betel leaf; Piper betle L AbstrakInfeksi bakteri menjadi masalah yang sudah sering terjadi dan berdampak buruk bagi kesehatan manusia dan hewan. Pengobatan untuk penyakit infeksi biasanya menggunakan antibiotik, namun banyak penggunaan antibiotik yang disalahgunakan sehingga dapat mengakibatkan terjadinya resistensi dan bakteri menjadi tidak sensitif terhadap pengobatan antibiotik. Daun sirih hijau merupakan salah satu obat tradisional yang memiliki kandungan minyak atsiri dan komponen utamanya terdiri fenol dengan beberapa turunannya yang berperan sebagai antibakteri. Hasil dari penelitian yang telah dilakukan dapat dinyatakan bahwa daun sirih hijau dapat berpotensi sebagai antibakteri. Selain itu daun sirih hijau mudah didapat dan memiliki toksisitas rendah yang menyebabkan tanaman tradisional tersebut dijadikan sebagai alternatif dalam mengobati infeksi bakteri. Studi literatur ini membahas daun sirih hijau (Piper betle L) sebagai antibakteri terhadap bakteri Gram positif dan Gram negatif meliputi komponen senyawa aktif dan mekanismenya.Kata kunci : aktivitas antibakteri; daun sirih hijau; Piper betle L","PeriodicalId":17708,"journal":{"name":"Jurnal Sain Veteriner","volume":"2 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-08-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"85574677","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Dedek Wahyuni, H. Latif, M. Sudarwanto, Chaerul Basri
Burung walet di Indonesia umumnya dibudidayakan pada rumah burung walet (RBW) secara tradisional dengan pola pemeliharaan tertentu. Pola pemeliharaan menjadi salah satu faktor pendukung bagi burung walet untuk memproduksi sarang burung walet (SBW) dengan kualitas baik secara berkelanjutan. Penelitian ini bertujuan untuk menganalisis pola pemeliharaan burung walet di RBW di pulau-pulau utama penghasil SBW di Indonesia. Suatu survei terhadap total 44 RBW di pulau Jawa, Sumatera, Sulawesi, dan Kalimantan telah dilakukan untuk mengetahui pola pemeliharaan di masing-masing pulau tersebut. Data dikumpulkan melalui wawancara secara langsung dengan menggunakan kuesioner terstruktur. Pertanyaan dalam kuesioner terdiri atas karakteristik bangunan, kebersihan, sumber makanan dan udara, dan lingkungan RBW. Hasil penelitian menunjukkan bahwa bangunan RBW umumnya bertingkat, dengan atap beton, dinding bata semen, lantai plester semen, dan sirip kayu. Rumah burung walet dibersihkan dibersihkan dengan cara digores/disapu dalam waktu kurang dari dua bulan. Kotoran burung walet umumnya digunakan untuk kebutuhan sendiri. Rumah burung walet umumnya tidak menyediakan bahan untuk menarik serangga atau pakan tambahan. Pakan burung walet umumnyaHymenoptera , dan sumber airnya adalah kolam di dalam gedung RBW. Lingkungan RBW merupakan kawasan pemukiman dan dekat dengan jalan raya. Pembinaan dan pemantauan terhadap pola pemeliharaan burung walet perlu terus dilakukan untuk mendapatkan SBW yang berkualitas baik.
{"title":"POLA PEMELIHARAAN BURUNG WALET PADA PULAU-PULAU UTAMA PENGHASIL SARANG BURUNG WALET DI INDONESIA","authors":"Dedek Wahyuni, H. Latif, M. Sudarwanto, Chaerul Basri","doi":"10.22146/jsv.69112","DOIUrl":"https://doi.org/10.22146/jsv.69112","url":null,"abstract":"Burung walet di Indonesia umumnya dibudidayakan pada rumah burung walet (RBW) secara tradisional dengan pola pemeliharaan tertentu. Pola pemeliharaan menjadi salah satu faktor pendukung bagi burung walet untuk memproduksi sarang burung walet (SBW) dengan kualitas baik secara berkelanjutan. Penelitian ini bertujuan untuk menganalisis pola pemeliharaan burung walet di RBW di pulau-pulau utama penghasil SBW di Indonesia. Suatu survei terhadap total 44 RBW di pulau Jawa, Sumatera, Sulawesi, dan Kalimantan telah dilakukan untuk mengetahui pola pemeliharaan di masing-masing pulau tersebut. Data dikumpulkan melalui wawancara secara langsung dengan menggunakan kuesioner terstruktur. Pertanyaan dalam kuesioner terdiri atas karakteristik bangunan, kebersihan, sumber makanan dan udara, dan lingkungan RBW. Hasil penelitian menunjukkan bahwa bangunan RBW umumnya bertingkat, dengan atap beton, dinding bata semen, lantai plester semen, dan sirip kayu. Rumah burung walet dibersihkan dibersihkan dengan cara digores/disapu dalam waktu kurang dari dua bulan. Kotoran burung walet umumnya digunakan untuk kebutuhan sendiri. Rumah burung walet umumnya tidak menyediakan bahan untuk menarik serangga atau pakan tambahan. Pakan burung walet umumnyaHymenoptera , dan sumber airnya adalah kolam di dalam gedung RBW. Lingkungan RBW merupakan kawasan pemukiman dan dekat dengan jalan raya. Pembinaan dan pemantauan terhadap pola pemeliharaan burung walet perlu terus dilakukan untuk mendapatkan SBW yang berkualitas baik. ","PeriodicalId":17708,"journal":{"name":"Jurnal Sain Veteriner","volume":"23 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-08-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"83637788","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
I. G. K. Oka Wirawan, Suryawati -, T. N. I. Koni, Redempta Wea
This research aims at determining the differences in the vermicidal activity between EADMAN and EADMDV in-vitro based on concentration and treatment time. It encompassed three groups of concentrations of EADMAN and EADMDV solution (2.5%, 3.5%, 4.5% from 0.2 g/mL each extract solution), a group of negative control (aqua pro injection), and a group of positive control (Oxfendazole 0.055%). The EADMAN and EADMDV was diluted in 1.5 mL aqua pro injection, then placed on petri dish with a concentration of 2.5%, 3.5%, 4.5% of 0.2 g/mL extract solution. Eight worms placed on each petri dish afterwards. The effectivity of EADMAN and EADMDV was determined based on the percentage of dead worms. The dead and live worms was counted from each immerse group for 1, 3, 5, 7 hours with four replications for each treatment. The vermicidal activity was indicated by touching the worms using a spatula; if it did not react, it was put in warm water. If the worms did not move, it was declared as the dead one, otherwise it was paralysed. Data of effective concentration from both extracts against vermicidal activity was analyzed using a two-way Anova, followed by Tukey test (P>0.05). The result showed the vermicidal activity of 2,5% EADMDV was significantly greater than 4.5% EADMDV, the negative control, and EADMAN at all concentrations. Meanwhile, its difference to 3.5% EADMDV and positive controls revealed insignificant results statistically. Therefore, 2.5% concentration of EADMDV with 7 hours of immersions gave the most effective vermicidal activity against Haemonchus contortus.
{"title":"The Vermicidal Activity of Water Extract Two Types of Ethnopharmacology Against Haemonchus contortus in Kacang Goat (Capra hircus) In-vitro","authors":"I. G. K. Oka Wirawan, Suryawati -, T. N. I. Koni, Redempta Wea","doi":"10.22146/jsv.70779","DOIUrl":"https://doi.org/10.22146/jsv.70779","url":null,"abstract":"This research aims at determining the differences in the vermicidal activity between EADMAN and EADMDV in-vitro based on concentration and treatment time. It encompassed three groups of concentrations of EADMAN and EADMDV solution (2.5%, 3.5%, 4.5% from 0.2 g/mL each extract solution), a group of negative control (aqua pro injection), and a group of positive control (Oxfendazole 0.055%). The EADMAN and EADMDV was diluted in 1.5 mL aqua pro injection, then placed on petri dish with a concentration of 2.5%, 3.5%, 4.5% of 0.2 g/mL extract solution. Eight worms placed on each petri dish afterwards. The effectivity of EADMAN and EADMDV was determined based on the percentage of dead worms. The dead and live worms was counted from each immerse group for 1, 3, 5, 7 hours with four replications for each treatment. The vermicidal activity was indicated by touching the worms using a spatula; if it did not react, it was put in warm water. If the worms did not move, it was declared as the dead one, otherwise it was paralysed. Data of effective concentration from both extracts against vermicidal activity was analyzed using a two-way Anova, followed by Tukey test (P>0.05). The result showed the vermicidal activity of 2,5% EADMDV was significantly greater than 4.5% EADMDV, the negative control, and EADMAN at all concentrations. Meanwhile, its difference to 3.5% EADMDV and positive controls revealed insignificant results statistically. Therefore, 2.5% concentration of EADMDV with 7 hours of immersions gave the most effective vermicidal activity against Haemonchus contortus.","PeriodicalId":17708,"journal":{"name":"Jurnal Sain Veteriner","volume":"38 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"87181624","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Hidayati Kumalasari, P. E. Sudaryatma, Artanti Tri Lestari, Wahyu Nurlita, W. Nugraha, Nur Hasanah, Ida Ayu Mirah Meliana Dewi, Ni Putu Arya Shintya Anggraeni
Indonesia become a maritime country with large of ocean area and beach line with marine species diversity reaches 37% of the world’s fish. This makes Indonesia have aquaculture potential that supported by an appropriate climate. However, the challenge of viral diseases caused by Infectious Spleen and Kidney Necrosis Virus (ISKNV) and Viral Nervous Necrosis (VNN) would hamper marine aquaculture and causing huge economic losses. Therefore, it is necessary to carry out early detection methods that are efficient, fast, precise and accurate to identifying these viral disease. Here we developed a multiplex-PCR (mPCR) a method that can detect simultaneously of the ISKNV and VNN. The results of this method showed high sensitivity and specificity by using MCP and CP gene target primers to detect ISKNV and VNN, respectively. From a total of 353 samples of seawater fish examined by mPCR, positive results following of single infection of ISKNV and VNN were 14 and 2, respectively. We also found that 18 grouper fish was co-infection with these viruses. From these results, it shows that the mPCR method developed has efficiency, faster and high accuracy. This founding was possible to be applied in laboratory testing or early detection system in the field for ISKNV and VNN.
{"title":"Infection of Infectious Spleen and Kidney Necrosis Virus and Viral Nervouse Necrosis in Seawater Fish Identifed Using Multiplex Polymerase Chain Reaction","authors":"Hidayati Kumalasari, P. E. Sudaryatma, Artanti Tri Lestari, Wahyu Nurlita, W. Nugraha, Nur Hasanah, Ida Ayu Mirah Meliana Dewi, Ni Putu Arya Shintya Anggraeni","doi":"10.22146/jsv.73599","DOIUrl":"https://doi.org/10.22146/jsv.73599","url":null,"abstract":"Indonesia become a maritime country with large of ocean area and beach line with marine species diversity reaches 37% of the world’s fish. This makes Indonesia have aquaculture potential that supported by an appropriate climate. However, the challenge of viral diseases caused by Infectious Spleen and Kidney Necrosis Virus (ISKNV) and Viral Nervous Necrosis (VNN) would hamper marine aquaculture and causing huge economic losses. Therefore, it is necessary to carry out early detection methods that are efficient, fast, precise and accurate to identifying these viral disease. Here we developed a multiplex-PCR (mPCR) a method that can detect simultaneously of the ISKNV and VNN. The results of this method showed high sensitivity and specificity by using MCP and CP gene target primers to detect ISKNV and VNN, respectively. From a total of 353 samples of seawater fish examined by mPCR, positive results following of single infection of ISKNV and VNN were 14 and 2, respectively. We also found that 18 grouper fish was co-infection with these viruses. From these results, it shows that the mPCR method developed has efficiency, faster and high accuracy. This founding was possible to be applied in laboratory testing or early detection system in the field for ISKNV and VNN. ","PeriodicalId":17708,"journal":{"name":"Jurnal Sain Veteriner","volume":"3 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"73161629","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
A. Ibrahim, W. Artama, R. Widayanti, B. A. Atmoko, I. Budisatria, D. Larasati
The accuracy of the estrus detection was important in sheep breeding management. This study aimed to determine the correlation between the farmers’ characteristics and the knowledge on estrus detection of their sheep. The data were collected by interviewing 33 farmers, who have raised sheep with a breeding system for at least two years of experience in Batur Village, Banjarnegara, Indonesia. The farmers’ knowledge for estrus detection was divided into three-level, namely low, medium, and high levels. The results showed that the farmers’ knowledge for estrus detection was 51.5%, 27.3%, and 21.2% on the low, medium, and high levels, respectively. There was a significant relationship between formal education, informal education, number of sheep, the sheepfold position, stay duration in the sheepfold, and the ram ownership for mating toward knowledge of estrus detection level. It can be concluded that there is a relationship between farmer characteristics in Batur Village and the ability to detect estrus in ewes, which is dominated at low levels, so it is necessary to increase farmers' knowledge regarding estrus detection.
{"title":"Farmers' Characteristics Associations with the Knowledge for Sheep Estrus Detection in Batur Village, Banjarnegara, Indonesia","authors":"A. Ibrahim, W. Artama, R. Widayanti, B. A. Atmoko, I. Budisatria, D. Larasati","doi":"10.22146/jsv.68462","DOIUrl":"https://doi.org/10.22146/jsv.68462","url":null,"abstract":"The accuracy of the estrus detection was important in sheep breeding management. This study aimed to determine the correlation between the farmers’ characteristics and the knowledge on estrus detection of their sheep. The data were collected by interviewing 33 farmers, who have raised sheep with a breeding system for at least two years of experience in Batur Village, Banjarnegara, Indonesia. The farmers’ knowledge for estrus detection was divided into three-level, namely low, medium, and high levels. The results showed that the farmers’ knowledge for estrus detection was 51.5%, 27.3%, and 21.2% on the low, medium, and high levels, respectively. There was a significant relationship between formal education, informal education, number of sheep, the sheepfold position, stay duration in the sheepfold, and the ram ownership for mating toward knowledge of estrus detection level. It can be concluded that there is a relationship between farmer characteristics in Batur Village and the ability to detect estrus in ewes, which is dominated at low levels, so it is necessary to increase farmers' knowledge regarding estrus detection.","PeriodicalId":17708,"journal":{"name":"Jurnal Sain Veteriner","volume":"10 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"88447693","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Z. Zulfan, S. Sugito, C. A. Fitri, H. Latif, Khairul Murdani
Abstrak Pencegahan dampak cekaman panas pada ayam broiler dapat dilakukan dengan pemberian ekstrak tanaman yang juga berguna dalam pengembangan obat-obat tradisional sebagai alternatif pengurangan penggunaan bahan-bahan kimiawi. Tujuan penelitian ini adalah untuk mengevaluasi performan ayam broiler yang diberikan ekstrak daun jaloh melalui air minum. Penelitian dilakukan di Laboratorium Lapangan Peternakan (LLP), Jurusan Peternakan, Fakultas Pertanian, Universitas Syiah Kuala. Sebanyak 80 ekor anak ayam broiler strain MB 202 produksi PT Japfa, Medan digunakan dalam penelitian ini. Perlakuan adalah suplementasi ekstrak daun jaloh melalui air minum pada dosis 0, 500, 1000, and 1500 ml/l air. Rancangan yang digunakan adalah Rancangan Acak Blok terdiri 4 perlakuan dan 4 blok. Tiap blok merupakan eksperimental unit yang masing-masing ditempati 5 ekor ayam. Hasil penelitian memperlihatkan walaupun secara statistik tidak berbeda nyata (P>0,05), suplementasi ekstrak daun jaloh di dalam air minum cenderung meningkatkan performan ayam broiler. Berat badan akhir, konsumsi ransum, dan konsumsi air minum meningkat sedangkan FCR menurun. Hasil penelitian disimpulkan bahwa pemberian ekstrak daun jaloh melalui air minum dapat menurunkan cekaman panas dan kematian ayam broiler dengan penggunaan terbaik pada pengenceran 1000 ml /l water air. Kata kunci: Jaloh; leaf, ekstrak, broiler, performan Abstract Prevent heat stress in broilers has been optionally created by using plants as starting points for drug development, specifically those used in traditional medicine. The objective of this study was to evaluate the performances of broiler chickens raised by offering jaloh leaf extract (JLE) through drinking water. The research has been conducted at the Field Laboratory of Animal Husbandry, Animal Husbandry Department, Syiah Kuala University. As many as 80 broiler chicks strain MB 202 produced by PT Japfa, Medan were used in this study. The treatment was supplementation JLE within drinking water at a dose of 0, 500, 1000, and 1500 ml/l of water, respectively. The research was performed into a Block Randomized Design (RBD), consisting of 4 treatments and 4 blocks. Each block was an experimental unit, each of which was placed by 5 chickens. The results of study indicated that although statistically no significant differences were detected (P>0.05) supplementation of JLE through drinking water had tendency to improve the performances of broilers. Final body weight, body weight gain, feed intake. and drinking water intake increased and FCR decreased. It was concluded that giving JLE through drinking water could minimize heat stress and reduce the mortality of broilers with the best provision of JLE at the level of 1000 ml /l water.Key words: Jaloh; leaf, extract, broiler, performances
{"title":"Improve the Performances of Broilers as Reduce Heat Stress by the Supplementation Jaloh (Salix tetrasperma Roxb) in Drinking Water","authors":"Z. Zulfan, S. Sugito, C. A. Fitri, H. Latif, Khairul Murdani","doi":"10.22146/jsv.59711","DOIUrl":"https://doi.org/10.22146/jsv.59711","url":null,"abstract":"Abstrak Pencegahan dampak cekaman panas pada ayam broiler dapat dilakukan dengan pemberian ekstrak tanaman yang juga berguna dalam pengembangan obat-obat tradisional sebagai alternatif pengurangan penggunaan bahan-bahan kimiawi. Tujuan penelitian ini adalah untuk mengevaluasi performan ayam broiler yang diberikan ekstrak daun jaloh melalui air minum. Penelitian dilakukan di Laboratorium Lapangan Peternakan (LLP), Jurusan Peternakan, Fakultas Pertanian, Universitas Syiah Kuala. Sebanyak 80 ekor anak ayam broiler strain MB 202 produksi PT Japfa, Medan digunakan dalam penelitian ini. Perlakuan adalah suplementasi ekstrak daun jaloh melalui air minum pada dosis 0, 500, 1000, and 1500 ml/l air. Rancangan yang digunakan adalah Rancangan Acak Blok terdiri 4 perlakuan dan 4 blok. Tiap blok merupakan eksperimental unit yang masing-masing ditempati 5 ekor ayam. Hasil penelitian memperlihatkan walaupun secara statistik tidak berbeda nyata (P>0,05), suplementasi ekstrak daun jaloh di dalam air minum cenderung meningkatkan performan ayam broiler. Berat badan akhir, konsumsi ransum, dan konsumsi air minum meningkat sedangkan FCR menurun. Hasil penelitian disimpulkan bahwa pemberian ekstrak daun jaloh melalui air minum dapat menurunkan cekaman panas dan kematian ayam broiler dengan penggunaan terbaik pada pengenceran 1000 ml /l water air. Kata kunci: Jaloh; leaf, ekstrak, broiler, performan Abstract Prevent heat stress in broilers has been optionally created by using plants as starting points for drug development, specifically those used in traditional medicine. The objective of this study was to evaluate the performances of broiler chickens raised by offering jaloh leaf extract (JLE) through drinking water. The research has been conducted at the Field Laboratory of Animal Husbandry, Animal Husbandry Department, Syiah Kuala University. As many as 80 broiler chicks strain MB 202 produced by PT Japfa, Medan were used in this study. The treatment was supplementation JLE within drinking water at a dose of 0, 500, 1000, and 1500 ml/l of water, respectively. The research was performed into a Block Randomized Design (RBD), consisting of 4 treatments and 4 blocks. Each block was an experimental unit, each of which was placed by 5 chickens. The results of study indicated that although statistically no significant differences were detected (P>0.05) supplementation of JLE through drinking water had tendency to improve the performances of broilers. Final body weight, body weight gain, feed intake. and drinking water intake increased and FCR decreased. It was concluded that giving JLE through drinking water could minimize heat stress and reduce the mortality of broilers with the best provision of JLE at the level of 1000 ml /l water.Key words: Jaloh; leaf, extract, broiler, performances","PeriodicalId":17708,"journal":{"name":"Jurnal Sain Veteriner","volume":"30 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-04-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"76892080","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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