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Oblique stagnation point flow of hybrid nanofluid on a stretching sheet with modified thermal conductivity models and Darcy's law 基于改进导热模型和达西定律的混合纳米流体在拉伸片上的斜滞止点流动
IF 1.1 4区 综合性期刊 Q3 MULTIDISCIPLINARY SCIENCES Pub Date : 2025-07-28 DOI: 10.1016/j.kjs.2025.100481
Z. Abbas , J. Hasnain , M.Y. Rafiq , M.A. Saeed , H. Shahzad
Hybrid nanofluids have demonstrated improved thermal performance and stability across a wide range of applications, including thermal efficiency systems, solar collectors, energy production, nuclear processes, and enhanced heat transfer. Motivated by these promising applications, this study investigates two-dimensional incompressible oblique stagnation point flow over a stretching/shrinking sheet embedded in a porous medium. The analysis compares two widely used thermal conductivity models, the Yamada-Ota and Xue models, for hybrid nanofluids composed of graphene oxide (GO) and iron oxide, magnetite (Fe3O4) nanoparticles dispersed in a non-Newtonian engine oil (EO) base fluid. Additionally, the influences of thermal radiation, heat generation/absorption, and chemical reactions are incorporated to explore the heat and mass transfer characteristics. The governing equations are transformed into a system of ordinary differential equations using similarity transformations, and the resulting system is solved numerically via the fourth-order Runge-Kutta-Fehlberg integration method combined with an efficient shooting technique. The results reveal that increasing the magnetic field strength enhances the fluid temperature while reducing its velocity. Higher heat generation parameters lead to intensified thermal distribution, while lower permeability increases velocity instability. Quantitatively, the Xue model demonstrates a higher surface heat transfer rate than the Yamada-Ota model, and a larger Schmidt number reduces mass transfer rates. These insights are particularly valuable for optimizing heat and mass transfer in porous thermal management systems.
混合纳米流体在热效率系统、太阳能集热器、能源生产、核过程和强化传热等广泛应用中表现出了更好的热性能和稳定性。在这些有前景的应用的激励下,本研究研究了嵌入多孔介质的拉伸/收缩薄片上的二维不可压缩斜驻点流动。该分析比较了两种广泛使用的导热模型,即Yamada-Ota和Xue模型,这两种模型是由分散在非牛顿发动机油(EO)基液中的氧化石墨烯(GO)和氧化铁、磁铁矿(Fe3O4)纳米颗粒组成的混合纳米流体。此外,还考虑了热辐射、热产生/吸收和化学反应的影响,以探索传热传质特性。利用相似变换将控制方程转化为常微分方程组,利用四阶Runge-Kutta-Fehlberg积分法结合高效射击技术对控制方程组进行数值求解。结果表明,增大磁场强度,流体温度升高,速度降低;较高的产热参数导致热分布加剧,而较低的渗透率则增加了速度不稳定性。定量地,Xue模型比Yamada-Ota模型显示出更高的表面传热率,更大的施密特数降低了传质率。这些见解对于优化多孔热管理系统中的传热和传质特别有价值。
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引用次数: 0
Fractional perspective of HH f-divergence, midpoint and trapezoidal type estimates for superquadratic function 超二次函数的分形发散、中点和梯形估计
IF 1.1 4区 综合性期刊 Q3 MULTIDISCIPLINARY SCIENCES Pub Date : 2025-07-28 DOI: 10.1016/j.kjs.2025.100452
Saad Ihsan Butt , Dawood Khan , Sanja Tipurić-Spužević , Bandar Bin Mohsin
In this study, we take into account the notion of superquadraticity and come up with the fractional version of midpoint and trapezoidal types estimations for differentiable superquadratic function via Riemann–Liouville’s fractional integral operators. It is to be noted that the inequalities derived from superquadratic functions exhibit greater refinement in comparison to those derived from convex functions. The findings are validated through simplified results, numerical analyses, and graphical representations, using a selection of suitable examples. We also introduce the Hermite–Hadamard (HH) f-divergence for superquadratic function and obtain its associate properties via Riemann–Liouville’s fractional integral operators. The work is enhanced with applications of modified Bessel’s function of Type-1 and Mittag-Leffler function which is another motivating factor. The new results significantly extend and enhance the existing work available in the literature.
本文考虑了超二次性的概念,利用Riemann-Liouville分数阶积分算子,给出了可微超二次函数的中点估计和梯形估计的分数阶形式。值得注意的是,由超二次函数导出的不等式比由凸函数导出的不等式更精细。通过选择合适的例子,简化结果、数值分析和图形表示验证了研究结果。引入了超二次函数的Hermite-Hadamard (HH) f-散度,并利用Riemann-Liouville分数阶积分算子得到了其相关性质。改进的贝塞尔函数(Type-1)和Mittag-Leffler函数(另一个激励因素)的应用加强了工作。新的结果显着扩展和加强现有的工作,可在文献。
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引用次数: 0
Cilia-assisted flow for the Johnson–Segalman fluid inside a convergent complex wavy passage with the magnetic field, hall effect, and porous medium 具有磁场、霍尔效应和多孔介质的复杂波状通道中Johnson-Segalman流体的纤毛辅助流动
IF 1.1 4区 综合性期刊 Q3 MULTIDISCIPLINARY SCIENCES Pub Date : 2025-07-28 DOI: 10.1016/j.kjs.2025.100480
Muhammad Waris Saeed Khan , Zeeshan Asghar , Wasfi Shatanawi , Muhammad Asif Gondal
This study investigates the cilia-driven flow of a Johnson–Segalman (J–S) non-Newtonian fluid through a two-dimensional converging channel under the influence of a vertically applied magnetic field. The problem is motivated by its relevance to biological fluid transport and the design of bio-inspired microfluidic devices. Key physical effects such as the Hall current, magnetic field interaction, and porous medium resistance are incorporated to reflect realistic conditions found in physiological and industrial settings. The governing equations, derived under the assumptions of low Reynolds number and long wavelength approximations, are numerically solved using the bvp4c method. The results reveal that the interplay between geometric convergence, complex ciliary wall undulations, magnetic forces, and Hall currents significantly alters the fluid's axial velocity, pressure gradient, and shear stress distributions. In particular, increases in the Darcy number and Hall parameter enhance fluid transport, while stronger magnetic fields and higher wall slip lengths tend to suppress flow. The novelty of this work lies in its comprehensive coupling of cilia-induced transport with Hall magnetohydrodynamics in a converging porous duct, an area scarcely explored in existing literature. These insights offer potential applications in optimizing next-generation microfluidic designs, peristaltic transport systems, and bio-mimetic pumping technologies.
本文研究了在垂直外加磁场作用下,由纤毛驱动的Johnson-Segalman (J-S)非牛顿流体在二维收敛通道中的流动。这个问题的动机是它与生物流体传输和仿生微流体装置的设计相关。关键的物理效应,如霍尔电流,磁场相互作用,和多孔介质电阻被纳入,以反映在生理和工业环境中发现的现实条件。在低雷诺数和长波长近似假设下导出的控制方程,采用bvp4c方法进行了数值求解。结果表明,几何收敛、复杂纤毛壁波动、磁力和霍尔电流之间的相互作用显著改变了流体的轴向速度、压力梯度和剪应力分布。特别是,达西数和霍尔参数的增加增强了流体的输运,而更强的磁场和更高的壁面滑移长度往往会抑制流动。这项工作的新颖之处在于它将纤毛诱导的输移与霍尔磁流体力学在会聚多孔管道中的综合耦合,这是现有文献中很少探索的领域。这些见解为优化下一代微流体设计、蠕动输送系统和仿生泵技术提供了潜在的应用。
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引用次数: 0
A new stochastic compartmental epidemic model driven by a multidimensional stochastic differential equation under a voluntary and imperfect vaccination strategy 非完全自愿接种策略下多维随机微分方程驱动的随机区室流行病模型
IF 1.1 4区 综合性期刊 Q3 MULTIDISCIPLINARY SCIENCES Pub Date : 2025-07-26 DOI: 10.1016/j.kjs.2025.100473
Benasquar Mohammed , Aissa Sghir , Dr. Said El Idrissi
This work introduces a novel compartmental epidemic model that integrates both deterministic and stochastic components to capture the dynamics of disease transmission under a voluntary and imperfect vaccination strategy. The model features a nonlinear incidence rate and differentiates between two classes of susceptible individuals: those who opt for vaccination and those who decline it. The deterministic structure is formulated as a system of multidimensional ordinary differential equations. To incorporate environmental fluctuations, the model is extended by introducing stochastic perturbations into the transmission rate via a white noise term, yielding a system of multidimensional stochastic differential equations. The basic reproduction number is derived to establish the conditions under which the disease either dies out or persists. The effects of the vaccination strategy, as well as the model’s sensitivity to parameter variations, are thoroughly analyzed. Furthermore, the existence and uniqueness of solutions are proven, and the model’s equilibria are characterized. Finally, numerical simulations are conducted to illustrate and validate the analytical results.
这项工作引入了一种新的区隔流行病模型,该模型集成了确定性和随机成分,以捕获自愿和不完善的疫苗接种策略下疾病传播的动力学。该模型以非线性发病率为特征,并区分了两类易感个体:选择接种疫苗的人和拒绝接种疫苗的人。确定性结构被表述为一个多维常微分方程系统。为了纳入环境波动,通过白噪声项将随机扰动引入传输速率来扩展模型,从而产生一个多维随机微分方程系统。导出基本繁殖数是为了确定疾病消灭或持续存在的条件。深入分析了疫苗接种策略的影响以及模型对参数变化的敏感性。进一步证明了模型解的存在唯一性,并刻画了模型的平衡点。最后通过数值模拟对分析结果进行了说明和验证。
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引用次数: 0
Application of scanning electron microscopy unraveling seed micromorphological characteristics in laticifers of family Apocynaceae and subfamily Asclepiadoideae 应用扫描电镜研究夹竹桃科和麻瓜亚科乳汁管种子微形态特征
IF 1.2 4区 综合性期刊 Q3 MULTIDISCIPLINARY SCIENCES Pub Date : 2025-07-21 DOI: 10.1016/j.kjs.2025.100477
Saeeda Naz , Moona Nazish , Jawaher Alzahrani , Haseeb-Ur Rehman , Fozia Bibi , Muhammad Zubair , Muhammad Ishfaq Khan , Muhammad Tahir Naseem , Wajid Zaman
The understanding of trait variation within the seed collection provides an understanding of genetic variation and knowledge about plant conservation. In this study, twelve species of the lactiferous family Apocynaceae and subfamily Asclepiadoideae have been evaluated for seed macro-micromorphological characterization. Seed surface texture, dimension, seed shape and sculpture, seed wing surface cell, coma position, level and color, hilum position and shape were examined by using a scanning electron microscope (SEM). This is the first study reporting coma surface sculpturing in Pakistan. Qualitative and quantitative features of seeds exhibited alteration among selected species of both families. The seed shapes were found oblong and flattened. Eight types of seed coat surface (reticulate-regular, reticulate-irregular, regular-rugose, aculeate, papillate, reticulate, ruminate, and striate), nine types of seed wing sculpturing, and four types of hilum surface cells that varied from reticulate to striate were observed. The maximum number of seed surfaces exhibited spiral to undulate hairy anticlinal wall ornamentation. The periclinal wall varied from smooth to warty. A taxonomic key was developed using seed morphological characters for the correct identification of lactiferous taxa. The variations among the studied species described the significance of SEM as a beneficial tool to vitalize the hidden micromorphological features among seeds of lactiferous taxa that eventually aid in seeds' accurate identification, delimitation, classification, and exploration in the future. Laticifer features are useful to understand the generic relationships within the family.
对种子收集性状变异的了解有助于了解遗传变异和植物保护知识。本研究对夹竹桃科和麻瓜亚科的12种种子进行了宏显微形态鉴定。采用扫描电子显微镜(SEM)对种子表面纹理、尺寸、种子形状和雕刻、种子翅表面细胞、彗发位置、水平和颜色、种脐位置和形状进行了观察。这是巴基斯坦首次报道昏迷表面雕刻的研究。两科所选种种子的定性和定量特征都发生了变化。种子的形状是椭圆形和扁平的。种皮表面有八种类型(网状-规则、网状-不规则、规则-褶皱、针状、乳头状、网状、反刍和条纹),九种类型的种子翼雕刻,四种类型的种脐表面细胞从网状到条纹不等。最大数量的种子表面呈螺旋状至波状的毛状背斜壁纹饰。周壁从光滑到疣状不等。利用种子的形态特征,建立了正确识别乳系分类群的分类钥匙。研究物种之间的差异说明了扫描电镜作为一种有益的工具的重要性,它可以激活哺乳类群种子中隐藏的微形态特征,最终有助于种子在未来的准确识别、划分、分类和探索。乳汁管的特征有助于理解家族内部的一般关系。
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引用次数: 0
Leveraging airport CCTV footage through video understanding techniques for visibility prediction 利用机场闭路电视录像,通过视频理解技术进行能见度预测
IF 1.2 4区 综合性期刊 Q3 MULTIDISCIPLINARY SCIENCES Pub Date : 2025-07-18 DOI: 10.1016/j.kjs.2025.100470
Zeonlung Pun , Xinyu Tian , Shan Gao
Accurate atmospheric visibility prediction is critical for enhancing airport safety, especially under adverse weather conditions. However, existing visibility prediction methods predominantly rely on single-image analysis, using either traditional image processing techniques or deep learning models, which often fail to fully capture the dynamic and temporal characteristics inherent in video data. In this study, we explore various video understanding models for visibility prediction, achieving promising results and pioneering the use of video understanding techniques in this domain. Unlike traditional static image-based methods, our proposed three-stream network model integrates spatial information from individual frames, motion dynamics through optical flow, and key reference points extracted via SIFT (scale-invariant feature transform) descriptors. This enables the model to capture both short-term and long-term environmental changes. Experimental results show that our three-stream network significantly outperforms single-frame and image-based models in predicting RVR_1A (average runway visibility range of one minute). Our model achieves an R2 mean of 0.896 and an accuracy mean of 0.860 on the test set, substantially outperforming traditional methods. These results not only demonstrate the superior performance of our approach in real-world scenarios but also highlight the potential of video understanding techniques for airport safety monitoring applications.
准确的大气能见度预测对加强机场安全至关重要,特别是在恶劣天气条件下。然而,现有的可见性预测方法主要依赖于单图像分析,使用传统的图像处理技术或深度学习模型,往往无法充分捕捉视频数据固有的动态和时间特征。在本研究中,我们探索了用于可见性预测的各种视频理解模型,取得了令人鼓舞的结果,并开创了视频理解技术在该领域的应用。与传统的基于静态图像的方法不同,我们提出的三流网络模型集成了来自单个帧的空间信息,通过光流的运动动态以及通过SIFT(尺度不变特征变换)描述符提取的关键参考点。这使模型能够捕捉短期和长期的环境变化。实验结果表明,我们的三流网络在预测RVR_1A(一分钟跑道平均能见度范围)方面明显优于单帧模型和基于图像的模型。我们的模型在测试集上的R2均值为0.896,准确率均值为0.860,大大优于传统方法。这些结果不仅证明了我们的方法在现实场景中的优越性能,而且突出了视频理解技术在机场安全监控应用中的潜力。
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引用次数: 0
Enhanced wheat growth and stress resilience under copper toxicity through ZnO─NPs and Azospirillum brasilense 氧化锌NPs和巴西氮螺旋菌对铜毒小麦生长和胁迫恢复能力的促进作用
IF 1.2 4区 综合性期刊 Q3 MULTIDISCIPLINARY SCIENCES Pub Date : 2025-07-17 DOI: 10.1016/j.kjs.2025.100476
Mohammad K. Okla , Sadia Javed , Muhammad Faran Tahir , Ibrahim A. Saleh , Ambreen Qadir , Muhammad Anas , Muhammad Hamzah Saleem , Temoor Ahmed , Abdulrahman Abdullah Alatar , Bandar M. AlMunqedhi , Shafaqat Ali
Copper (Cu) pollution in agricultural soils is considered a serious health risk due to its accumulation in plants, but fewer studies have been conducted on its effects and alleviation strategies by using nanoparticles (NPs) and plant growth-promoting rhizobacteria (PGPR). Taking into consideration the positive effects of zinc oxide nanoparticles (ZnO─NPs) and Azospirillum brasilense in reducing Cu toxicity in plants, the present study was conducted. A pot experiment was conducted to determine the effects of the application of different levels of 25 and 50 ppm of ZnO─NPs and A. brasilense i.e., 10 and 20 ppm, on Cu accumulation, morpho-physiological and antioxidative defense attributes of wheat (Triticum aestivum L.) exposed to severe Cu stress (0, 100, and 200 mg kg−1). Results from the present study showed that the increasing levels of Cu in the soil significantly (P < 0.05) decreased plant growth and biomass, photosynthetic pigments, gas exchange attributes, sugars, and nutritional contents from the roots and shoots of the plants. In contrast, increasing levels of Cu in the soil significantly (P < 0.05) increased oxidative stress indicators in terms of malondialdehyde, hydrogen peroxide, and electrolyte leakage, and also increased organic acid exudation patterns in the roots of T. aestivum. Although the activities of enzymatic antioxidants and the response of their gene expressions in the roots and shoots of the plants and non-enzymatic ones, such as phenolic, flavonoid, ascorbic acid, and anthocyanin contents, were increased by increasing the Cu concentration in the soil. The negative impacts of Cu injury were reduced by the application of ZnO─NPs and A. brasilense, which increased plant growth and biomass, improved photosynthetic apparatus, antioxidant enzymes, and mineral uptake, as well as diminished the exudation of organic acids and oxidative stress indicators in roots of T. aestivum by decreasing Cr toxicity. Research findings, therefore, suggest that the application of ZnO─NPs and A. brasilense can ameliorate Cu toxicity in T. aestivum, resulting in improved plant growth and composition under metal stress, as depicted by balanced exudation of organic acids.
农业土壤中的铜(Cu)污染由于其在植物体内的积累而被认为是严重的健康风险,但利用纳米颗粒(NPs)和植物促生长根瘤菌(PGPR)对其影响和缓解策略的研究较少。考虑到氧化锌纳米颗粒(ZnO─NPs)和巴西氮螺旋菌(Azospirillum brasilense)在降低植物铜毒性方面的积极作用,进行了本研究。通过盆栽试验,研究了施用25和50 ppm氧化锌(NPs)和10和20 ppm巴西小麦(A. brasilense)对铜胁迫(0、100和200 mg kg - 1)下小麦(Triticum aestivum L.) Cu积累、形态生理和抗氧化防御特性的影响。本研究结果表明,土壤中Cu含量显著增加(P <;0.05)降低了植物的生长和生物量、光合色素、气体交换属性、糖以及植物根和芽的营养含量。相反,土壤中Cu含量显著增加(P <;0.05)增加了丙二醛、过氧化氢和电解质泄漏等氧化应激指标,并增加了柽柳根部有机酸的渗出模式。土壤中Cu浓度的增加增加了植物根、芽中酶促抗氧化剂活性及其基因表达的响应,增加了非酶促抗氧化剂的酚类、类黄酮、抗坏血酸和花青素含量。氧化锌─NPs和巴西木通过降低Cr毒性,提高了巴西木的生长和生物量,改善了光合机构、抗氧化酶和矿物质的吸收,减少了巴西木根系有机酸的分泌和氧化胁迫指标,从而减轻了铜胁迫对巴西木的负面影响。因此,研究结果表明,ZnO─NPs和A. brasilense的应用可以改善铜对T. aestivum的毒性,从而改善金属胁迫下植物的生长和组成,如有机酸的平衡渗出所示。
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引用次数: 0
A compact structure of four half-circle resonator metasurfaces for microwave sensing applications 用于微波传感应用的四个半圆谐振腔超表面的紧凑结构
IF 1.2 4区 综合性期刊 Q3 MULTIDISCIPLINARY SCIENCES Pub Date : 2025-07-16 DOI: 10.1016/j.kjs.2025.100474
Khalid Saeed Lateef Al-Badri , Moayed H. Hameed , Shihab A. Shawkat , Abdulsattar Abdullah Hamad
This work presents a simple and compact quadrilateral half-circle resonator metasurface for microwave sensing applications. The metasurface (MTS) unit cell exhibits carrier resonance surface characteristics for the K-band. It has an excellent absorption response at fo = 21.45 GHz and at fo = 23.04 GHz, indicating its coherence and efficiency. The proposed unit cell was developed for sensing applications to detect the sensitivity of liquid or solid materials within an electromagnetic simulation environment at K-band. The sensing methods are investigated to determine which is more effective for measuring sensitivity and its potential in microwave sensing. The resonance frequency fluctuates based on permittivity and refractive index. The MTS unit cell amplifies this resonance using electric field intensity. The measured results demonstrate that the proposed metasurface has excellent sensitivity, a high Q-factor (Q > 55), and a strong Figure of Merit (FoM). The good sensitivity, Q-factor, and FoM results indicate that the proposed MTS is a viable solution for microwave sensing applications.
本文提出了一种简单紧凑的四边形半圆谐振腔超表面,用于微波传感应用。超表面(MTS)单元电池表现出k波段的载流子共振表面特性。它在fo = 21.45 GHz和fo = 23.04 GHz处具有优异的吸收响应,表明了它的相干性和效率。所提出的单元电池是为传感应用而开发的,用于检测k波段电磁模拟环境中液体或固体材料的灵敏度。研究了几种传感方法,以确定哪种方法更有效地测量灵敏度及其在微波传感中的潜力。谐振频率根据介电常数和折射率波动。MTS单元电池利用电场强度放大这种共振。测量结果表明,所提出的超表面具有优异的灵敏度,高Q因子(Q >;55),以及强大的功勋图(FoM)。良好的灵敏度、q因子和FoM结果表明,所提出的MTS是一种可行的微波传感解决方案。
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引用次数: 0
GC-MS, FTIR, XRD, UV–Vis, and HPLC profiling of the natural bioactive constituents of Lemna minor L. extract and its in-vitro antimicrobial and anti-parasitic activities against pathogenic organisms GC-MS、FTIR、XRD、UV-Vis和HPLC分析了柠檬草提取物的天然活性成分及其对病原菌的体外抗菌和抗寄生活性
IF 1.2 4区 综合性期刊 Q3 MULTIDISCIPLINARY SCIENCES Pub Date : 2025-07-15 DOI: 10.1016/j.kjs.2025.100469
Shabnum Shaheen , Moneeza Abbas , Riffat Siddique , Zaryab Khalid , Tahira Alyas , Sana Khalid , Jawaher Alzahrani , Nadia Ghani , Sobia Sarwar , Asif Kamal , Moona Nazish , Maximilian Lackner
The current study focuses on Lemna minor L. (common duckweed) through proximate analysis, qualitative and quantitative phytochemical analysis by UV spectroscopy (UV), gas chromatography and mass spectroscopy (GC-MS), Fourier-transform infrared (FTIR) spectroscopy, and X-ray diffraction analysis (XRD). The phytochemical investigation of the fresh sample of L. minor was compared with the powdered sample extracted using different drying procedures. The highest moisture content was recorded in fresh samples (87.94 %), while maximum percentages of ash, fiber, and protein were found in oven-dried powder as 14.81 %, 24.01 %, and 34.92 %, respectively. The freeze-dried powder contained the greatest percentages of fat (8.48 %) and carbohydrates (17.5 %). The qualitative phytochemical analysis detected variable concentrations of bioactive compounds. Quantitative analysis revealed that tannins were found in the maximum concentration across all solvents, while non-polar or polar steroids were extracted the least. In terms of mineral content, the freeze-dried plant samples contained the maximum levels of potassium (54.51 mg/100g), followed by phosphorus (31.750.25 mg/100g), magnesium (18.25 ± 0.25 mg/100g) and sodium (4.4 ± 1 mg/100g). The maximum antibacterial potential was achieved against E. coli, that was 21.4 mm zone of inhibition (ZOI). Similarly, the maximum fungal inhibition was observed against A. niger, which was 21 mm ZOI at the highest concentration of 75 μl. The parasite's promastigote and amastigote forms each had a significant death rate of 75.40 ± 1.16 and 60.15 ± 0.12 at 150 g/mL, respectively.
目前的研究重点是通过近似分析、紫外光谱(UV)、气相色谱和质谱(GC-MS)、傅里叶变换红外光谱(FTIR)和x射线衍射分析(XRD)等方法对普通浮萍进行定性和定量的植物化学分析。对不同干燥方法提取的粉末样品和新鲜样品的植物化学性质进行了比较。新鲜样品的水分含量最高(87.94%),干粉的灰分、纤维和蛋白质含量最高(14.81%、24.01%和34.92%)。冻干粉的脂肪含量最高(8.48%),碳水化合物含量最高(17.5%)。定性植物化学分析检测到不同浓度的生物活性化合物。定量分析表明,在所有溶剂中单宁含量最高,而非极性或极性类固醇的提取量最少。在矿物质含量方面,冻干植物样品中钾含量最高(54.51 mg/100g),其次是磷(31.750.25 mg/100g)、镁(18.25±0.25 mg/100g)和钠(4.4±1 mg/100g)。对大肠杆菌的抑菌潜力最大,为21.4 mm的抑制区(ZOI)。同样,在最高浓度为75 μl时,对黑曲霉的抑制效果为21 mm。在150 g/mL浓度下,虫体的promastigoi和amastigoi的死亡率分别为75.40±1.16和60.15±0.12。
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引用次数: 0
Protective effect of aqueous methanolic bark extracts of Alstonia scholaris (L.) R. Br. against RSL3-induced ferroptosis 金菖蒲树皮甲醇水提物的保护作用r . Br。抗rsl3诱导的铁下垂
IF 1.2 4区 综合性期刊 Q3 MULTIDISCIPLINARY SCIENCES Pub Date : 2025-07-14 DOI: 10.1016/j.kjs.2025.100472
Katrina Lorraine C. Chua , Anne Nicole S. Tensuan , Mica Xiena Yungca , Charisse T. Tugahan , Virgilio T. Linis , Rafael A. Espiritu
Ferroptosis is an iron-dependent form of non-apoptotic cell death that has been implicated in various human pathophysiological conditions and its regulation may have therapeutic potential. Plants have been a rich source of bioactive compounds targeting various diseases, and extracts from Alstonia scholaris (L.) R. Br., locally known as “Dita”, are known for their biological activities such as antioxidant, anti-inflammatory, and hepatoprotective properties. However, its ability to regulate cell death, specifically ferroptosis, has not yet been explored. In this study, the antioxidant potential of A. scholaris bark extract and its effects in the execution of ferroptosis in HT-29 cell line were investigated. 2,2-diphenyl-1-picrylhydrazyl (DPPH) assay demonstrated that A. scholaris extract possesses significant radical scavenging activity (IC50 = 0.24 mg/mL) which may be accounted for by its modest amount of total phenolic (26.83 ± 3.51 mg gallic acid equivalent/g crude extract) and flavonoid (7.59 ± 1.59 mg quercetin equivalent/g crude extract) compounds. At a concentration of 0.1875 mg/mL, the extract rescued HT-29 cells against RAS-selective lethal 3 (RSL3)-induced ferroptosis to levels comparable with the standard inhibitor ferrostatin-1. Gas chromatography-mass spectrometry (GC-MS) and liquid chromatography-mass spectrometry (LC-MS) analyses suggest the possible presence of quinic acid and magnolol, respectively, both of which could contribute to the observed antioxidant property of the A. scholaris extract. Finally, to explore the possible role of quinic acid and magnolol in inhibiting ferroptosis, molecular docking simulations with heme oxygenase 1 (HO-1) were carried out which demonstrated preferable interactions that were comparable with a co-crystalized inhibitor (binding free energy, BFE = −7.64 kcal/mol), particularly magnolol (BFE = −7.22 kcal/mol). These results demonstrate the potential of finding novel ferroptosis regulators in A. scholaris extracts, nevertheless, further studies are needed to conclusively confirm both the presence and activity of these compounds in the extract.
铁下垂是一种铁依赖性的非凋亡细胞死亡形式,与各种人类病理生理状况有关,其调节可能具有治疗潜力。植物中含有丰富的针对多种疾病的生物活性化合物,而Alstonia scholaris (L.)r . Br。在当地被称为“Dita”,以其生物活性而闻名,如抗氧化、抗炎和保护肝脏的特性。然而,其调节细胞死亡的能力,特别是铁下垂,尚未被探索。本研究探讨了槐皮提取物的抗氧化作用及其对HT-29细胞凋亡的影响。2,2-二苯基-1-苦味酰肼(DPPH)测定结果表明,黄芪提取物具有显著的自由基清除活性(IC50 = 0.24 mg/mL),这可能是由于其总酚(26.83±3.51 mg没食子酸当量/g粗提物)和类黄酮(7.59±1.59 mg槲皮素当量/g粗提物)含量有限所致。在0.1875 mg/mL的浓度下,提取物对ras -选择性致死3 (RSL3)诱导的铁死亡的HT-29细胞的保护水平与标准抑制剂铁抑素-1相当。气相色谱-质谱联用(GC-MS)和液相色谱-质谱联用(LC-MS)分析表明,槲皮提取物中可能分别含有奎宁酸和厚朴酚,这两种物质可能与槲皮提取物的抗氧化作用有关。最后,为了探索奎宁酸和厚朴酚在抑制铁死亡中的可能作用,研究人员进行了与血红素加氧酶1 (HO-1)的分子对接模拟,结果表明,与共晶抑制剂(结合自由能,BFE =−7.64 kcal/mol),特别是厚朴酚(BFE =−7.22 kcal/mol)的相互作用相当。这些结果表明,有可能发现新的铁下垂调节剂在金针叶提取物,然而,需要进一步的研究来最终确认这些化合物在提取物中的存在和活性。
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Kuwait Journal of Science
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