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Properties and applications of the Tan Weibull loss distribution Tan Weibull 损失分布的性质和应用
IF 1.2 4区 综合性期刊 Q3 MULTIDISCIPLINARY SCIENCES Pub Date : 2024-07-24 DOI: 10.1016/j.kjs.2024.100304

Probability models play crucial role in modeling loss in the finance and actuarial sciences. In this article, a new family of loss distributions known as the Tan F-Loss family of distributions is proposed with the Tan Weibull Loss distribution as a special case. The density exhibits decreasing, right skewed, symmetric, and approximately symmetric shapes. The hazard rate function shows decreasing and modified bathtub shapes. The statistical properties of the Tan Weibull Loss distribution including the quantile function, moments, expansion of the general rate, moment generating function, incomplete moment, and order statistics are studied. The maximum likelihood estimators of the distribution are also studied. Simulations are carried out to examine the behavior of the estimators. The results show that the estimators are consistent. The usefulness of the proposed distribution is demonstrated with two insurance loss datasets. The results show that the proposed distribution gives a better parametric to the two datasets compared with the competing distributions.

概率模型在金融和精算科学的损失建模中发挥着至关重要的作用。本文以 Tan Weibull 损失分布为特例,提出了一个新的损失分布族,即 Tan F-Loss 分布族。其密度呈现递减、右倾、对称和近似对称的形状。危险率函数呈现递减和修正的浴缸形状。研究了 Tan Weibull Loss 分布的统计特性,包括量化函数、矩、一般率的扩展、矩产生函数、不完全矩和阶次统计。还研究了该分布的最大似然估计值。通过模拟来检验估计器的行为。结果表明,估计值是一致的。利用两个保险损失数据集证明了所提出的分布的实用性。结果表明,与其他同类分布相比,所提出的分布为两个数据集提供了更好的参数。
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引用次数: 0
Chitosan (CTS) induced secondary metabolite production in Canscora decussata Schult. - An endangered medicinal plant 壳聚糖(CTS)诱导 Canscora decussata Schult.- 一种濒危药用植物
IF 1.2 4区 综合性期刊 Q3 MULTIDISCIPLINARY SCIENCES Pub Date : 2024-07-24 DOI: 10.1016/j.kjs.2024.100306

Bioengineering has excellent potential in plant-based in vitro cultures for efficiently synthesizing desired plant products. Due to the rapid destruction of natural habitats, in vitro methods might save endangered species. Winged-Stem Canscora is an endangered medicinal herb used in traditional medicine for brain disorders. This study investigates the induction of in-vitro metabolite production using chitosan. Among the various concentrations of auxins tested, 82% of callus was obtained from Murashige and Skoog (MS) medium with 3 mg L−1 Kinetin (KIN) + 1 mg L−1 1-Naphthaleneacetic acid (NAA). The highest callus content was after the 3rd subculture at 200 mg L−1 chitosan treatment. On the 70th day of culture, levels of total phenolics and flavonoids are increased in the elicited and normal callus. However, antioxidant activity was higher in elicited callus compared to normal callus and wild plants. Higher secondary metabolite concentrations of elicited callus have superior anti-bacterial and anti-fungal activity. Secondary metabolites from elicited callus have a lower inhibitory concentration than those from wild plant and normal callus. High-performance thin layer chromatography (HPTLC) analysis showed that the elicited callus had higher amounts of mangiferin (0.178 μg mL−1) and scopoletin (0.133 μg mL−1). An efficient approach was employed to ensure that sufficient amounts of the widely used plant secondary metabolites from medicinal plants, such as mangiferin and scopoletin, were accessible to meet demand.

生物工程在以植物为基础的体外培养方面具有巨大潜力,可有效合成所需的植物产品。由于自然栖息地迅速遭到破坏,体外培养方法有可能拯救濒危物种。翅茎苍术是一种濒危药材,在传统医学中用于治疗脑部疾病。本研究利用壳聚糖诱导体外代谢物的产生。在测试的各种浓度的辅助剂中,82%的胼胝体是从含有 3 mg L-1 Kinetin (KIN) + 1 mg L-1 1-Naphthaleneacetic acid (NAA) 的 Murashige and Skoog (MS) 培养基中获得的。第 3 次亚培养后,在 200 mg L-1 的壳聚糖处理下,胼胝体含量最高。在培养的第 70 天,诱导胼胝体和正常胼胝体的总酚类和类黄酮含量都有所增加。然而,与正常胼胝体和野生植物相比,诱导胼胝体的抗氧化活性更高。诱导胼胝体中次生代谢物浓度较高,具有较强的抗菌和抗真菌活性。与野生植物和正常胼胝体相比,诱导胼胝体中次生代谢物的抑制浓度较低。高效薄层色谱(HPTLC)分析表明,诱导胼胝体中的芒果苷(0.178 μg mL-1)和莨菪亭(0.133 μg mL-1)含量较高。该研究采用了一种高效的方法,以确保从药用植物中获得足够数量的广泛使用的植物次生代谢物,如芒果苷和莨菪亭,以满足需求。
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引用次数: 0
Antimicrobial, phytochemical, and antioxidant characterization of the leaf extracts of Clematis montana and Clematis grata 铁线莲(Clematis montana)和大铁线莲(Clematis grata)叶提取物的抗菌、植物化学和抗氧化特性分析
IF 1.2 4区 综合性期刊 Q3 MULTIDISCIPLINARY SCIENCES Pub Date : 2024-07-23 DOI: 10.1016/j.kjs.2024.100305

The worldwide emergence of multidrug-resistant bacterial infections among the population has impaired the existing effectiveness of antimicrobial treatments, necessitating the hunt for other alternatives. We aimed to find the antibacterial and antioxidant activities of Clematis montana and C. grata leaf extracts (both species have medicinal use among the local population). Extracts were assessed against four pathogenic bacterial strains (Escherichia coli, Salmonella typhi, Pseudomonas aeruginosa, and Staphylococcus aureus), through the agar well diffusion method. Total flavonoid content (TFC), total phenolic content (TPC), and 2,2-diphenyl-1-picrylhydrazyl (DPPH) scavenging activity were quantified. Results suggested that all plant extracts, particularly those extracted with methanol, were potentially active in suppressing pathogenic bacterial growth (particularly against P. aeruginosa and E. coli) although their efficiency varied when compared to vancomycin. However, the antibacterial activity of methanolic extracts from both species was not greatly influenced by the method of extraction (maceration/soxhlet apparatus). TFC was significantly higher in the methanolic extracts of both species in comparison to the n-hexane extracts, whereas TPC and DPPH scavenging activity were detected to be highest in the methanolic extract from the leaves of C. grata. Our results suggested that the leaf extracts from both Clematis species, particularly the methanolic-based extract of C. montana, might serve as a promising source of an effective antibacterial agent.

全球范围内出现的耐多药细菌感染损害了现有抗菌药物的疗效,因此有必要寻找其他替代品。我们的目的是研究铁线莲和 C. grata(这两种植物在当地人中都有药用价值)叶片提取物的抗菌和抗氧化活性。我们通过琼脂井扩散法评估了提取物对四种致病细菌菌株(大肠杆菌、伤寒沙门氏菌、绿脓杆菌和金黄色葡萄球菌)的抗菌活性。对总黄酮含量(TFC)、总酚含量(TPC)和 2,2-二苯基-1-苦基肼(DPPH)清除活性进行了量化。结果表明,所有植物提取物,尤其是用甲醇提取的植物提取物,在抑制病原菌生长(尤其是铜绿假单胞菌和大肠杆菌)方面都具有潜在的活性,但与万古霉素相比,它们的效率各不相同。不过,这两个物种的甲醇提取物的抗菌活性受提取方法(浸渍/索氏提取器)的影响不大。与正己烷提取物相比,两种植物的甲醇提取物的 TFC 都明显较高,而从 C. grata 的叶片中提取的甲醇提取物的 TPC 和 DPPH 清除活性最高。我们的研究结果表明,这两种铁线莲的叶提取物,尤其是 C. montana 的甲醇提取物,可能是一种很有前景的有效抗菌剂来源。
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引用次数: 0
An application of textual document classification for Arabic governmental correspondence 阿拉伯文政府公文的文本文件分类应用
IF 1.2 4区 综合性期刊 Q3 MULTIDISCIPLINARY SCIENCES Pub Date : 2024-07-22 DOI: 10.1016/j.kjs.2024.100299

The automation of classifying Arabic documents is becoming increasingly in demand, especially when dealing with an ever-growing amount of linguistic data. Natural language processing (NLP) has recently become one of the most significant fields in artificial intelligence (AI) thanks to recent advances in introducing transformer-based models. Transformers facilitate the use of reusable models by using pre-trained models (PTMs). This study aims to fine-tune monolingual (AraBERT (Antoun et al., 2020)), bilingual (GigaBERT (Lan et al., 2020)), and multilingual (XLM-RoBERTa (Conneau et al., 2020)) transformer-based encoder models to classify official Arabic correspondence in pre-defined classes and compare their predictive performance in terms of accuracy, using a new balanced dataset. The new balanced dataset has 22,741 Arabic texts and is categorized into six categories labeled with the most common ministries’ names. The results in this study show that GigaBERT achieved the highest accuracy rate of 98%. The implemented models may contribute to the domain of information systems (ISs) to facilitate the classification process in ministries without human intervention.

阿拉伯语文档的自动化分类需求日益增长,尤其是在处理日益增长的语言数据时。由于最近在引入基于转换器的模型方面取得了进展,自然语言处理(NLP)最近已成为人工智能(AI)中最重要的领域之一。转换器通过使用预训练模型(PTM)促进了可重用模型的使用。本研究旨在微调单语(AraBERT (Antoun et al., 2020))、双语(GigaBERT (Lan et al., 2020))和多语种(XLM-RoBERTa (Conneau et al., 2020))基于变换器的编码器模型,使用新的平衡数据集将阿拉伯语官方信函划分为预定义的类别,并比较它们在准确性方面的预测性能。新的平衡数据集包含 22,741 个阿拉伯语文本,并以最常见的部委名称将其分为六类。研究结果表明,GigaBERT 的准确率最高,达到 98%。所实施的模型可为信息系统(IS)领域做出贡献,从而在无需人工干预的情况下促进部委分类过程。
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引用次数: 0
Correlation, path-coefficient and principal component analysis association among quantitative traits in strawberry to unlock potential of vertical farming system 草莓数量性状之间的相关性、路径系数和主成分分析,以挖掘垂直耕作系统的潜力
IF 1.2 4区 综合性期刊 Q3 MULTIDISCIPLINARY SCIENCES Pub Date : 2024-07-22 DOI: 10.1016/j.kjs.2024.100303

The study explores the complex relationship between quantitative and qualitative characteristics in strawberries grown using a vertical farming system and artificial full spectrum light (AFSL). The research reveals the interdependencies between traits, identifies direct, and indirect effects on yield and quality, and highlights critical factors influencing overall variation in strawberry characteristics using correlation analysis, path-coefficient analysis, and principal component analysis (PCA). These discoveries open the door for specialized breeding and cultivation techniques that optimize vertical farming procedures and increase the sustainability and productivity of strawberries. This study confirmed the suitability of a four-layered vertical farming system for strawberry cultivation; however, an additional supply of AFSL at lower levels of verticals ensures a higher yield. The number of fruits per plant and average berry weight had a high (pij > 0.3) degree of association with yield per plant at genotypic and phenotypic levels. The principal component analysis revealed a close association of T1 (top level of vertical with natural light only), T2 (third level from the top of vertical with natural light only), and T5 (third level from the top of vertical with natural light and AFSL for 2 h) with yield contributing traits (number of bud formation, number of flowers, number of fruits per plant, fruit setting (%), fruit volume, average berry weight, average yield, and estimated yield per hectare).

这项研究探讨了使用垂直耕作系统和人工全光谱光(美标所)种植草莓的定量和定性特征之间的复杂关系。研究利用相关性分析、路径系数分析和主成分分析(PCA)揭示了性状之间的相互依存关系,确定了对产量和质量的直接和间接影响,并强调了影响草莓整体特性变化的关键因素。这些发现为专业化育种和栽培技术打开了大门,从而优化了垂直栽培程序,提高了草莓的可持续性和生产率。这项研究证实了四层垂直耕作系统对草莓栽培的适用性;然而,在垂直耕作的较低层额外供应美标所可确保更高的产量。在基因型和表型水平上,单株果实数和平均浆果重量与单株产量的关联度很高(pij > 0.3)。主成分分析表明,T1(仅有自然光的垂直顶层)、T2(仅有自然光的垂直顶层第三层)和 T5(自然光和美标所照射 2 小时的垂直顶层第三层)与产量贡献性状(花芽形成数、花朵数、单株果实数、坐果率(%)、果实体积、平均浆果重量、平均产量和每公顷估计产量)密切相关。
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引用次数: 0
Identification of algD and oprL genes in Pseudomonas aeruginosa isolated from multiple environments and different local sources in Iraq 从伊拉克多种环境和不同地方来源分离的铜绿假单胞菌中鉴定 algD 和 oprL 基因
IF 1.2 4区 综合性期刊 Q3 MULTIDISCIPLINARY SCIENCES Pub Date : 2024-07-21 DOI: 10.1016/j.kjs.2024.100302

The aim of this study was to investigate the spread of the genes algD and oprL in P. aeruginosa within the genetic structure of environmental sources of isolates and various clinical studies, and to evaluate a successful alternative for diagnosing Pseudomonas aeruginosa (P. aeruginosa) among various sources. In this study, 100 samples were collected from various clinical and environmental sources. Twenty-eight isolates of P. aeruginosa bacteria were isolated. Nine pathological samples were taken from wounds, burns, blood, and ear infections in three governorates: Salah al-Din, Baghdad, and Kirkuk. Five isolates of P. aeruginosa bacteria were collected from contaminated water from the Salah Al-Din and Baghdad governorates. The isolates were diagnosed phenotypically through the colony's shape, colour, odour and interaction with Gram stain. Additionally, the VITEK 2 system was used to confirm the diagnosis of the isolates. The results show that all the isolates belonged to the bacteria P. aeruginosa. The results of the molecular diagnosis using a polymerase chain reaction (PCR) technique for three genes with specialised primers (16SrRNA, algD, and oprL) show that most of the bacterial isolates from different sources belong to the type P. aeruginosa because they contain the specific gene 16SrRNA except for five isolates (M5, M7, M8, M9 and W1). Four of these belong to isolates with a pathogenic source and one was isolated from water. The results of the PCR for the algD gene show that all isolates contain the gene except for four diseased samples. Samples M5, M7, M8, and M9 were without the gene, while all isolates had the oprL gene. A sequencing analysis of the DNA was then performed for the isolates that were not associated with the specialised primers of the above genes to ensure their genera (M5, M7, M8, and M9), in addition to a sample of 15 isolated from soil (S6) that was associated with all special primers for the same genes for the purpose of comparison. The results show that isolates M5, M7, M8 and M9 were diseased isolates belonging to Escherichia coli, while the 15 isolated from soil (S6) were diagnosed as P. aeruginosa.

本研究旨在调查铜绿假单胞菌(P. aeruginosa)中的基因 algD 和 oprL 在环境分离物来源和各种临床研究的遗传结构中的传播情况,并评估在各种来源中诊断铜绿假单胞菌(P. aeruginosa)的成功替代方法。本研究从各种临床和环境来源收集了 100 份样本。分离出 28 株铜绿假单胞菌。9 份病理样本取自三个省的伤口、烧伤、血液和耳部感染:萨拉赫丁、巴格达和基尔库克。从萨拉赫丁省和巴格达省受污染的水中采集了 5 个铜绿假单胞菌分离物。通过菌落的形状、颜色、气味以及与革兰氏染色法的相互作用对分离菌进行了表型诊断。此外,还使用 VITEK 2 系统对分离物进行确诊。结果显示,所有分离物都属于铜绿假单胞菌。利用聚合酶链式反应(PCR)技术,使用专用引物对三个基因(16SrRNA、algD 和 oprL)进行分子诊断的结果表明,除了五个分离物(M5、M7、M8、M9 和 W1)外,大多数从不同来源分离的细菌都属于铜绿假单胞菌,因为它们都含有 16SrRNA 的特异基因。其中四个属于致病源分离物,一个是从水中分离出来的。藻胆 D 基因的 PCR 检测结果表明,除 4 个患病样本外,所有分离物都含有该基因。样本 M5、M7、M8 和 M9 没有该基因,而所有分离物都有 oprL 基因。然后对与上述基因的专用引物无关的分离物(M5、M7、M8 和 M9)的 DNA 进行了测序分析,以确定其属种,此外还对从土壤中分离出来的 15 个样本(S6)进行了测序分析,该样本与相同基因的所有专用引物都有关联,以便进行比较。结果表明,M5、M7、M8 和 M9 分离物是属于大肠杆菌的病原分离物,而从土壤(S6)中分离的 15 个样本被诊断为铜绿假单胞菌。
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引用次数: 0
Assessing low-cost sensor for characterizing temporal variation of PM2.5 in Bandung, Indonesia 评估用于描述印度尼西亚万隆 PM2.5 时空变化的低成本传感器
IF 1.2 4区 综合性期刊 Q3 MULTIDISCIPLINARY SCIENCES Pub Date : 2024-07-20 DOI: 10.1016/j.kjs.2024.100297

Fine particulate matter (PM2.5) is a concern due to its health effects, necessitating critical monitoring for both quantity and variability. Utilizing low-cost sensors to track PM2.5 is essential to augment other monitoring instruments, which are effective in generating temporal and spatial data. Therefore, in this study, we employed the low-cost PurpleAir (low-cost PA-II) to characterize the seasonal and diurnal variation of PM2.5 in Bandung, Indonesia, representing the densely populated metropolitan area of Bandung. During the sampling period from June 2022 to May 2023, co-location sampling with the filter-based Super Speciation Air Sampling System (SuperSASS) was employed to assess the low-cost PA-II. The PM2.5 data collected by the low-cost PA-II were compared to the SuperSASS data. The annual average mass concentration of PM2.5 measured by SuperSASS and the low-cost sensor was 31.51 ± 15.53 μg/m3 and 39.04 ± 15.16 μg/m3, respectively, surpassing the Indonesian government's regulation limit for an annual average of 15 μg/m3. The comparative results of the two methods were obtained with R2 = 0.96, and low-cost PA-II data was 1.24 higher than SuperSASS. The difference may be attributed to several factors, including differences in sensor technology, calibration, location, and data processing. The seasonal variation indicated an increase in concentration during the dry season and a decrease during the wet season. The diurnal pattern indicates the morning peak between 06:00 and 08:00 during the rush hour, as well as the evening peak between 18:00 and 23:00, attributed to low temperatures and stagnant conditions. The diurnal pattern of PM2.5, which often exhibits the lowest peak at midday, is influenced by a combination of meteorological, atmospheric, and human activity factors. The findings suggested that the utilization of low-cost PA-II for a broader spatial scope is promising for real-time monitoring of PM2.5, to increase air pollution awareness in society.

细颗粒物(PM2.5)因其对健康的影响而备受关注,因此有必要对其数量和变异性进行关键监测。利用低成本传感器来跟踪 PM2.5 对增强其他监测仪器至关重要,这些仪器能有效地生成时间和空间数据。因此,在本研究中,我们采用了低成本的 PurpleAir(低成本 PA-II)来描述印尼万隆 PM2.5 的季节和昼夜变化特征,万隆是印尼人口稠密的大都市地区。在 2022 年 6 月至 2023 年 5 月的采样期间,使用基于过滤器的超级物种空气采样系统(SuperSASS)进行了同地采样,以评估低成本 PA-II。低成本 PA-II 收集的 PM2.5 数据与 SuperSASS 数据进行了比较。SuperSASS 和低成本传感器测得的 PM2.5 年平均质量浓度分别为 31.51 ± 15.53 μg/m3 和 39.04 ± 15.16 μg/m3,超过了印尼政府规定的年平均 15 μg/m3 的限值。两种方法的比较结果为 R2 = 0.96,低成本 PA-II 数据比 SuperSASS 高 1.24。这种差异可归因于多种因素,包括传感器技术、校准、位置和数据处理方面的差异。季节变化表明,旱季浓度上升,雨季浓度下降。昼夜模式表明,由于气温低和停滞条件,高峰时段的早高峰为 6:00 至 8:00,晚高峰为 18:00 至 23:00。PM2.5 的昼夜模式通常在正午呈现最低峰值,受到气象、大气和人类活动因素的综合影响。研究结果表明,在更大的空间范围内利用低成本的 PA-II 实时监测 PM2.5,以提高社会对空气污染的认识是很有前景的。
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引用次数: 0
First-principles calculations to investigate structural, electronic, magnetic, thermodynamic, and thermoelectric properties of RbSrZ (Z = Ge and Sn) d0-d0 half-Heuslers for renewable energy applications 通过第一性原理计算研究用于可再生能源应用的 RbSrZ(Z = Ge 和 Sn)d-d 半赫兹勒的结构、电子、磁性、热力学和热电特性
IF 1.2 4区 综合性期刊 Q3 MULTIDISCIPLINARY SCIENCES Pub Date : 2024-07-19 DOI: 10.1016/j.kjs.2024.100300

The aim of this study is to examine the thermoelectric properties alongside the structural, electronic, and magnetic properties of RbSrGe and RbSrSn d0-d0 half Heuslers. The full potential-linearized augment plane wave (FP-LAPW) system and Boltzmann transport equation are employed, utilizing classical Boltzmann approximation (CBA) in the background of density functional theory (DFT) as configured within WIEN2K. The structure optimization employed the PBE approximations and the Murnaghan equation of state, leading to a stable phase for RbSrGe and RbSrSn. The observed negative formation energy implies that the synthesis of RbSrGe and RbSrSn are possible. The half Heusler compounds under investigation were found to exhibit a narrow band gap in the majority spin direction and metallic character in the minority, which classifies these materials as half-metallic alloys based on the analysis of their electronic properties. The examination of elastic properties reveals that RbSrGe is brittle and RbSrSn is ductile. Critical analysis of thermoelectric properties demonstrates that RbSrGe and RbSrSn half Heuslers are possible materials for thermoelectrical applications.

本研究的目的是在研究掺镱硒锗和掺镱硒 d0-d0 半 Heuslers 的结构、电子和磁性能的同时,研究它们的热电性能。在 WIEN2K 中配置的密度泛函理论(DFT)背景下,利用经典玻尔兹曼近似(CBA),采用了全电位线性化增强平面波(FP-LAPW)系统和玻尔兹曼输运方程。结构优化采用了 PBE 近似值和 Murnaghan 状态方程,从而为 RbSrGe 和 RbSrSn 确定了稳定相。观察到的负形成能意味着 RbSrGe 和 RbSrSn 的合成是可能的。研究发现,半 Heusler 化合物在多数自旋方向上表现出窄带隙,而在少数自旋方向上表现出金属特性,因此根据对其电子特性的分析,可将这些材料归类为半金属合金。对弹性特性的研究表明,掺杂锶的锗是脆性的,而掺杂锶的锰是韧性的。对热电性能的严格分析表明,掺镱锰锗和掺镱锰硒的半 Heuslers 是可能用于热电应用的材料。
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引用次数: 0
Control of the size distribution of AuNPs for colorimetric sensing by pulsed laser ablation in liquids 通过脉冲激光烧蚀控制液体中用于比色传感的 AuNPs 的尺寸分布
IF 1.2 4区 综合性期刊 Q3 MULTIDISCIPLINARY SCIENCES Pub Date : 2024-07-18 DOI: 10.1016/j.kjs.2024.100294

Gold nanoparticles (AuNPs) are extensively employed in colorimetric sensing, taking advantage of their optical properties to detect variables via observable color changes. These properties are primarily driven by localized surface plasmon resonance (LSPR), particularly pronounced in AuNPs within the visible spectrum. In this study, AuNPs were synthesized via pulsed laser ablation in liquids (PLAL) with a laser pulse energy (Ep) ranging from 25 mJ to 75 mJ. Size distributions, hydrodynamic diameters, polydispersity indices (PDI), absorbance intensity, and LSPR were characterized. Spherical AuNPs with FCC structure were synthesized, exhibiting a maximum absorption peak centered at approximately 529 nm wavelength and a size range between 50 nm and 178 nm, easily adjustable depending on the laser pulse energy used in the synthesis process. An anomalous behavior was noted at Ep=50 mJ, exhibiting three peaks in size distribution, high PDI, low absorbance intensity, and indistinct LSPR. By extending the ablation time from 10 min to 30 min, particle size decreased alongside lower PDI. Size distributions transitioned from three to two peaks, absorbance increased, and LSPR became readily identifiable. These findings underscore the size control over AuNP characteristics achievable through PLAL synthesis parameters, promising significant implications for the optimization of colorimetric sensor design and development.

金纳米粒子(AuNPs)被广泛应用于比色传感,利用其光学特性通过可观察到的颜色变化来检测变量。这些特性主要是由局部表面等离子体共振(LSPR)驱动的,在可见光谱范围内的 AuNPs 尤为明显。本研究通过液体脉冲激光烧蚀(PLAL)合成了 AuNPs,激光脉冲能量(Ep)从 25 mJ 到 75 mJ 不等。对其粒度分布、流体力学直径、多分散指数(PDI)、吸光度和 LSPR 进行了表征。合成出了具有 FCC 结构的球形 AuNPs,其最大吸收峰的中心波长约为 529 nm,尺寸范围在 50 nm 至 178 nm 之间,可根据合成过程中使用的激光脉冲能量轻松调节。在 Ep=50 mJ 时出现了异常行为,表现出三个尺寸分布峰、高 PDI、低吸光度和不清晰的 LSPR。将烧蚀时间从 10 分钟延长到 30 分钟后,颗粒尺寸减小,PDI 降低。粒度分布从三个峰过渡到两个峰,吸光度增加,LSPR 变得易于识别。这些发现强调了通过 PLAL 合成参数可实现对 AuNP 特性的粒度控制,有望对比色传感器的优化设计和开发产生重大影响。
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引用次数: 0
A statistical approach to study anatomical changes of pink guava cultivar (Psidium guajava L. cv Arka Kiran) during its ripening at the room temperature storage 研究粉红番石榴(Psidium guajava L. cv Arka Kiran)在室温储藏成熟过程中的解剖变化的统计方法
IF 1.2 4区 综合性期刊 Q3 MULTIDISCIPLINARY SCIENCES Pub Date : 2024-07-16 DOI: 10.1016/j.kjs.2024.100295

The ripening of climacteric fruit like guava is a complex process that is highly coordinated with its cellular backbone. In the present study, we combined microscopy, spectrophotometry, and statistical analysis to evaluate the anatomical changes in the pink variety of guava during five ripening stages (pre-ripe, ripe, color-turn, half over-ripe, and over-ripe) during its storage at room temperature (28±2 °C). The cholorophyll content of the peel, as determined by the measurement of chlorophyll a, b, and total chlorophyll, showed a significant decrease during the maturation process (4.05, 4.53, and 8.62 μg/cm2, respectively, in the pre-ripe stage to ‘not detectable’ in the over-ripe stage). Gradual loss of integrity of the fruit pulp (pericarp) from the preserved bee-hive structure to cell mass was also monitored by studying the cellular anatomy with brightfield and scanning electron microscopy. The epidermal thickness and width of the cortical parenchyma cells revealed statistical differences from the initial pre-ripe stage to the final full-ripe stage. Finally, based on the cellular dimensions, multivariate analysis using PCA (Principal Component Analysis) tool grouped the stages into three clusters, namely, pre-ripe: ripe, color-turn: half-over ripe, and over-ripe stages. In conclusion, this study provided significant insights into cultivar-specific anatomical changes in guava fruit, with potential for future research to develop variants with longer post-harvest storage life.

番石榴等气候性水果的成熟是一个复杂的过程,与其细胞骨架高度协调。在本研究中,我们结合显微镜、分光光度法和统计分析,对粉红番石榴在室温(28±2 °C)贮藏期间的五个成熟阶段(预熟、成熟、转色、半熟和过熟)的解剖变化进行了评估。根据叶绿素 a、b 和总叶绿素的测定结果,果皮的叶绿素含量在成熟过程中显著下降(从成熟前的 4.05、4.53 和 8.62 μg/cm2 到过熟阶段的 "检测不到")。此外,还通过明视野和扫描电子显微镜研究细胞解剖结构,监测果肉(果皮)从保存的蜂巢结构到细胞块的完整性逐渐丧失的情况。皮层实质细胞的表皮厚度和宽度显示了从最初的预成熟阶段到最后的完全成熟阶段的统计学差异。最后,根据细胞维度,利用 PCA(主成分分析)工具进行多元分析,将各阶段分为三组,即成熟前、转色:半熟和过熟阶段。总之,这项研究为了解番石榴果实中特定栽培品种的解剖变化提供了重要依据,为未来研究开发采后贮藏期更长的变种提供了潜力。
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Kuwait Journal of Science
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