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Effect of Fatty Alcohol and 6-Benzyladenine on Fruit Set and Fruit Quality of ‘Fuji’ Apples 脂肪醇和6-苄基腺苷对富士苹果坐果和果实品质的影响
4区 农林科学 Q3 HORTICULTURE Pub Date : 2023-02-28 DOI: 10.7235/hort.20230004
Hyun-Woo Shin, Jingi Yoo, Woo-Sung Kim, Jung-Geun Kwon, Myo Win Nay, In-Kyu Kang
This study was investigated the effect of fatty alcohol (FA) and 6-benzyladenine (6-BA) thinning treatments on the fruit set and fruit quality attributes of ‘Fuji/M.9’ apples. Trees were treated with FA (single, ×500 dilution), FA (double), 6-BA (×1,000 dilution), 6-BA (×500 dilution), FA (single) + 6-BA (×1,000 dilution), FA (double) + 6-BA (×500 dilution), and carbaryl. FA (80.5% a.i.) treatment was applied at 80% blossom of terminal flowers and 60–80% blossom of lateral flowers, and 6-BA (7.4% a.i.) and carbaryl treatments were applied at 8-mm size of central fruit. The total fruit set rate per cluster was observed 61.0% for terminal flowers and 37.3% for lateral flowers in the control but it was decreased to 35.2–50.6% for terminal flowers and 9.7–23.8% for lateral flowers in the thinning treatments, respectively. The rate of central fruit per terminal flower cluster was observed 92.6% in the control but it was 65.2–94.4% in FA and 6-BA treatments. However, the rate of non-fruit set per terminal flower cluster was observed 2.9% in the control but it was increased to 7.5–14.2% in the thinning treatments, and the rate of one fruit set per terminal flower cluster was 14.1% in the control but it was increased to 20.6–33.9% in the thinning treatments. In addition, the rate of non-fruit set per lateral flower cluster was observed 19.6% in the control but it was decreased to 32.2–69.0% in the thinning treatments. The fruit quality attributes were not different in all treatment groups at harvest, except fruit weight. Therefore, our results reveal that FA and 6-BA treatments have the significant effects in reducing the fruit rate in ‘Fuji’ apple.
本试验研究了脂肪醇(FA)和6-苄基ladenine (6-BA)稀释处理对富士/M坐果性状和果实品质的影响。9的苹果。用FA(单剂,×500稀释)、FA(双剂)、6-BA (×1,000稀释)、6-BA (×500稀释)、FA(单剂)+ 6-BA (×1,000稀释)、FA(双剂)+ 6-BA (×500稀释)和西维因处理树木。顶花开花80%和侧花开花60-80%处施用FA (a.i为80.5%)处理,中心果8mm处施用6-BA (a.i为7.4%)和西威因处理。顶花和侧花每簇总坐果率在对照中分别为61.0%和37.3%,间伐处理的顶花和侧花每簇总坐果率分别为35.2% ~ 50.6%和9.7% ~ 23.8%。对照每顶花序中心果率为92.6%,FA和6-BA处理的中心果率为65.2-94.4%。未坐果率在对照中为2.9%,间伐处理提高到7.5 ~ 14.2%;间伐处理提高到20.6 ~ 33.9%,间伐处理提高到14.1%。此外,每侧花簇不坐果率在对照中为19.6%,在间伐处理中下降到32.2 ~ 69.0%。除果实重量外,各处理组果实品质性状无显著差异。综上所述,FA和6-BA处理对‘富士’苹果的果率有显著的降低作用。
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引用次数: 0
Prediction of Fruit Maturity with Starch Pattern Index in Korean Apple Cultivars 用淀粉模式指数预测韩国苹果品种果实成熟度
4区 农林科学 Q3 HORTICULTURE Pub Date : 2023-02-28 DOI: 10.7235/hort.20230003
Jingi Yoo, Jung-Geun Kwon, In-Kyu Kang
This study was conducted to evaluate the starch loss pattern and to estimate the maturity indices and harvest time of the five Korean apple cultivars (‘RubyS’, ‘Arisoo’, ‘Hwangok’, ‘Picnic’, and ‘Colorpple’) in association with the changes of fruit quality attributes using a Cornell starch pattern index (SPI) analysis method (1-8 scores) and SPI chart development for domestic use. In 5 apple cultivars, the increase in SPI was directly related with the increase in fruit weight and soluble solids content and the decrease in flesh firmness and titratable acidity. For fruit peel coloration, the decrease in L* (indicator for brightness) and b* (indicator for yellowness) was strongly associated with the increase in SPI. In addition, the increase in a* (indicator for redness) was associated with the increase in SPI in all apple cultivars, except ‘Hwangok’, a yellow apple cultivar. A slight increase in internal ethylene concentration was also observed in all cultivars while the SPI was increased. Interestingly, the changes of SPI were different depending on each apple cultivar. Averagely, ‘RubyS’, ‘Arisoo’, ‘Hwangok’, and ‘Picnic’ apples took 3, 4, 5, and 7 days, and 7, 5, 7, and 7 days to change the SPI from 6 to 7 and from 7 to 8 scores, respectively. However, ‘Colorpple’ apple took about 10 days each to change the SPI from 6 to 7 and from 7 to 8 scores. Therefore, this study suggested that the development of SPI chart could be used as basic data to estimate the fruit maturity and harvest time of domestic apple cultivars.
本研究采用康奈尔淀粉模式指数(Cornell starch pattern index, SPI)分析方法(1-8分)和国内应用的SPI图表,评价了5个韩国苹果品种(“RubyS”、“Arisoo”、“hwang gok”、“Picnic”和“colorapple”)的淀粉损失模式、成熟指数和收获时间与果实品质属性变化的关系。在5个苹果品种中,SPI的增加与果实重量和可溶性固形物含量的增加、果肉硬度和可滴定酸度的降低有直接关系。果皮颜色的L*(亮度指标)和b*(黄度指标)的降低与SPI的增加密切相关。此外,a*(红度指标)的增加与所有苹果品种SPI的增加有关,但黄谷品种除外。随着SPI的增加,所有品种的内乙烯浓度也略有增加。有趣的是,不同苹果品种间SPI的变化是不同的。“红宝石”、“阿里苏”、“黄谷”、“野餐”等苹果的SPI从6分变为7分、从7分变为8分,平均需要3、4、5、7天,平均需要7、5、7天。然而,“colorapple”苹果花了大约10天的时间将SPI从6分提高到7分,从7分提高到8分。因此,本研究建议,开发SPI图可作为国内苹果品种果实成熟度和采收期估算的基础数据。
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引用次数: 2
Fingerprint Construction and Genetic Diversity Analysis of Tree Peony Collected from Hunan Province Based on SSR Markers 基于SSR标记的湖南牡丹指纹图谱构建及遗传多样性分析
4区 农林科学 Q3 HORTICULTURE Pub Date : 2021-10-01 DOI: 10.7235/HORT.20210061
Zhen-Yu Yang, Yang Shi, Jiaqi Shuai, Yanlin Li, Tian-Yong Sun, Mengjie Chen, Changping Lv
To protect and identify the varieties of tree peony, DNA fingerprints were constructed and genetic diversity was analyzed with simple sequence repeat markers with tailed primer M13 (TP-M13-SSR) technology in 18 local tree peony varieties in Hunan Province of China. Twenty-four out of 131 pairs of polymorphic primers showing stable amplification and good capillary electrophoresis peaks were selected. The Shannon information indexes (I), polymorphism information contents (PICs), and differentiation rates of eight primer pairs were higher than average. Unweighted pair-group method with arithmetic mean (UPGMA) dendrograms indicated that the genetic similarity coefficients of the 18 Hunan tree peony varieties ranged from 0.57 to 0.86, and Paeonia suffruticosa ‘Ning Xiang Hong’ (0.57) was classified as a separate group. The 18 varieties could be completely distinguished with two pairs of primers. The variety names, flower types, fingerprinting codes, and other information were included in the quick response (QR) code, which provides a basis for the molecular identification of Hunan tree peony varieties. The construction of DNA fingerprinting of tree peony varieties in Hunan is important for its protection. Additional key words: capillary electrophoresis, molecular markers, quick response (QR) code, simple sequence repeat with tailed primer M13 (TP-M13-SSR), unweighted pair-group method with arithmetic mean (UPGMA)
为了保护和鉴定牡丹品种,利用尾引物M13(TP-M13-SSR)技术构建了湖南省18个地方牡丹品种的DNA指纹图谱,并利用简单序列重复标记对其遗传多样性进行了分析。从131对多态性引物中筛选出24对具有稳定扩增和良好毛细管电泳峰的引物。8对引物的Shannon信息指数(I)、多态性信息含量(PIC)和分化率均高于平均水平。采用算术平均(UPGMA)树状图的未加权配对群方法,表明18个湖南牡丹品种的遗传相似系数在0.57~0.86之间,将牡丹‘宁香红’(0.57)分为一个单独的类群。用两对引物可以完全区分这18个品种。快速响应码中包含了品种名称、花型、指纹码等信息,为湖南牡丹品种的分子鉴定提供了依据。湖南牡丹品种DNA指纹图谱的构建对其保护具有重要意义。附加关键词:毛细管电泳、分子标记、快速反应(QR)码、带尾引物M13的简单序列重复(TP-M13-SSR)、算术平均的未加权配对群法(UPGMA)
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引用次数: 0
Enhancement of Growth and Antioxidant Enzyme Activities on Kimchi Cabbage by Melatonin Foliar Application under High Temperature and Drought Stress Conditions 高温干旱胁迫下叶面施褪黑素对白菜生长和抗氧化酶活性的促进作用
4区 农林科学 Q3 HORTICULTURE Pub Date : 2021-10-01 DOI: 10.7235/HORT.20210052
Jin Hyoung Lee, Heesoo Lee, S. Wi, I. Yu, K. Yeo, S. An, Y. Jang, S. Jang
Melatonin, an indoleamine molecule, regulates a wide range of physiological functions during the growth, morphogenesis, and response of plants to abiotic stresses. We examined the effect of exogenous melatonin application (5, 10, and 20 mg·L -1 ) on the growth and antioxidant enzyme activity of Kimchi cabbage under high temperature (air temperature 32/26°C) and drought stress (soil moisture 20–24%) conditions. Application of 10 mg·L -1 melatonin alleviated stress-induced growth inhibition and increased the shoot fresh weight and the leaf surface area of seedlings by 22.7% and 13.9%, respectively, compared to the control under the stress condition. Indeed, foliar application of melatonin at 10 mg·L -1 caused an increase of net photosynthetic rate and transpiration rate under the stress condition. The lowest level of H2O2 content was also observed at 10 mg·L -1 concentration of melatonin treatment, while no significant alterations in lipid peroxidation or concentrations of malondialdehyde were detected by treatment with different concentrations of melatonin. In addition, significantly elevated activity levels of ascorbate peroxidase (APX) and superoxide dismutase (SOD) were measured at 10 mg·L -1 melatonin treatment without significant changes in ascorbic acid (AsA) and proline contents under the stress condition. These results suggest that foliar application of 10 mg·L -1 melatonin ameliorates physiological damages caused by the combination of water deficit and heat stress and enhances the activity of the antioxidant enzymes such as APX and SOD, thereby improving tolerance against the water deficit and heat stress in Kimchi cabbage. Additional key words: antioxidant enzyme, Brassica rapa L, foliar application, photosynthesis, superoxide dismutase
褪黑激素是一种吲哚胺分子,在植物生长、形态发生和对非生物胁迫的反应过程中调节着广泛的生理功能。我们研究了在高温(气温32/26°C)和干旱胁迫(土壤湿度20-24%)条件下,外源褪黑素(5、10和20mg·L-1)对泡菜白菜生长和抗氧化酶活性的影响。施用10mg·L-1褪黑激素减轻了胁迫引起的生长抑制,使幼苗的地上部鲜重和叶表面积分别比对照增加22.7%和13.9%。事实上,在胁迫条件下,叶面施用10mg·L-1的褪黑激素可提高净光合速率和蒸腾速率。在10 mg·L-1浓度的褪黑素处理下,H2O2含量最低,而不同浓度的褪黑素对脂质过氧化和丙二醛浓度没有显著影响。此外,在应激条件下,10mg·L-1褪黑素处理的抗坏血酸过氧化物酶(APX)和超氧化物歧化酶(SOD)活性显著升高,抗坏血酸(AsA)和脯氨酸含量没有显著变化。这些结果表明,叶面施用10mg·L-1褪黑激素可以改善由缺水和热胁迫共同引起的生理损伤,并提高APX和SOD等抗氧化酶的活性,从而提高泡菜对缺水和热应激的耐受性。附加关键词:抗氧化酶,油菜,叶面应用,光合作用,超氧化物歧化酶
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引用次数: 5
Analysis of Changes in Growth, Nutrient Absorption Characteristics, and Glucosinolate Contents of Chinese Cabbage (Brassica rapa pekinensis) Grown in Reclaimed Land according to Irrigation with Different Salt Concentrations 不同盐浓度灌溉对开垦地白菜生长、养分吸收特性及硫代葡萄糖苷含量的影响
4区 农林科学 Q3 HORTICULTURE Pub Date : 2021-10-01 DOI: 10.7235/HORT.20210051
I. Lee, D. Jung, P. Yi, Jun Gu Lee, S. Bhandari
Reclaimed land contains a large amount of soluble salts and exchangeable sodium in the soil, so it is necessary to select and cultivate tolerant crops that are resistant against salt stress. Chinese cabbage, a staple ingredient in kimchi, is an important vegetable that accounts for most of the production area of leafy vegetables in Korea, but no cases have been reported of growing cabbages in reclaimed land to examine growth and glucosinolate content. The objective of this study was to analyze the growth, nutrient absorption characteristics, and glucosinolate content of Chinese cabbages grown in spring and autumn on reclaimed land with irrigation water with different salt concentrations. The salt concentrations of the irrigation water were 0, 2, and 4 dS∙m -1 . Growth of spring and autumn Chinese cabbages did not change according to the salt concentration of the irrigation water, and only autumn Chinese cabbages showed an increase of Na content according to the salt concentration of the irrigation water. The total glucosinolate content tended to decrease when the salt concentration of irrigation water increased. The increase in salt concentration of irrigation water may reduce the bitterness of autumn Chinese cabbage. Here, it was confirmed that Chinese cabbage cultivation is possible even if the salt concentration of irrigation water reaches 4dS·m -1 on reclaimed land, and the glucosinolate content of autumn Chinese cabbage decreases when salt concentration increases. Additional key words: macronutrient, micronutrient, salt stress, secondary metabolite, sodium chloride
开垦后的土地土壤中含有大量的可溶性盐和可交换钠,因此有必要选择和培育耐盐作物。大白菜是泡菜的主要成分,是一种重要的蔬菜,占韩国叶菜生产面积的大部分,但目前还没有在开垦的土地上种植大白菜以检测其生长和硫代葡萄糖苷含量的报道。本研究的目的是分析不同盐浓度的灌溉水对春秋季开垦地上种植的大白菜的生长、营养吸收特性和硫代葡萄糖苷含量的影响。灌溉水的盐浓度分别为0、2和4dS∙m-1。春、秋两季白菜的生长不随灌溉水盐分浓度的变化而变化,只有秋两季的白菜Na含量随灌溉水的盐分浓度的增加而增加。随着灌溉水含盐量的增加,硫代葡萄糖苷总含量呈下降趋势。灌溉水中盐分浓度的增加可以减少秋白菜的苦味。结果表明,即使开垦地灌溉水的盐分浓度达到4dS·m-1,也可以种植大白菜,秋季大白菜的硫代葡萄糖苷含量随着盐分浓度的增加而降低。附加关键词:常量营养素、微量营养素、盐胁迫、次生代谢产物、氯化钠
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引用次数: 1
Variability of Chrysanthemum Cultivars Induced by Gamma Irradiation γ辐照对菊花品种变异的影响
4区 农林科学 Q3 HORTICULTURE Pub Date : 2021-10-01 DOI: 10.7235/HORT.20210059
Saika Anne, Jin Hee Lim
This study aimed to determine the exposure dose of gamma radiation on stem cuttings of chrysanthemum cultivars. Explants of 11 cultivars (‘Eldora’, ‘Rania’, ‘Aileen’, ‘Olga pink’, ‘Sevda’, ‘Heimish’, ‘Rania’, ‘Attirance’, ‘White Plume’, ‘Coral King’, and ‘Leporem’) were subjected to different levels of gamma irradiation (0, 10, 30, 50, 70, 100, and 150 Gy). The results revealed that variation in vegetative growth parameters, viz., plant height, stem diameter, number of leaves, leaf length, leaf width, total number of flowers, flower diameter, number of petals, petal length and width, and number of buds occurred in the M1V1 generation compared to the control (0 Gy). The survival rates of the gamma-irradiated cultivars changed dramatically. The number of surviving plantlets based on 50% lethal dose (LD50) was found in 100 and 150 Gy treated plants 60 days after radiation. In addition, the highest percentage of plant survival was observed in the control, while the lowest percentage was observed in plants irradiated with 100 Gy and 150 Gy, along with delayed bud initiation and flowering. The number of days from planting to flowering was low in all of the control cultivars, even though the flowering percentage was high in the control compared to the treatments. The highest number of branches was observed in plants that were subjected to 100 and 150 Gy treatment, while 10, 30, and 50 Gy gamma irradiation revealed similar vegetative characteristics to all the control cultivars. Maximum leaf abnormality and leaf color variation were observed in the 70, 100, and 150 Gy treatments. Mutant varieties were produced from those treated with 10, 30, 50, 70, and 150 Gy, in which a higher number of mutants and mutation frequency were found in the 30 and 70 Gy treatments. The results showed that more flower color mutations occurred due to higher exposure to irradiation with minimum alteration in the vegetative growth in all cultivars. These results establish a standard to identify the gamma radiation dose that can induce mutations in chrysanthemum. Additional key words: flower color, growth parameter, lethal dose, mutation frequency, stem cutting, vegetative growth
本研究旨在测定γ辐射对菊花品种茎插条的暴露剂量。对11个品种(“Aldora”、“Rania”、“Aileen”、“Olga pink”、“Sevda”、“Heimish”、“拉尼娅”、“Attrance”、“White Plume”、“Coral King”和“Leporem”)的外植体进行不同水平的γ辐照(0、10、30、50、70、100和150 Gy)。结果表明,与对照(0Gy)相比,M1V1代的营养生长参数,即株高、茎径、叶数、叶长、叶宽、总花数、花径、花瓣数、花瓣长宽和芽数发生了变化。γ辐照品种的存活率发生了显著变化。在辐射60天后,在100和150Gy处理的植物中发现了基于50%致死剂量(LD50)的存活植株的数量。此外,在对照中观察到最高的植物存活率,而在100Gy和150Gy照射的植物中观察到最低的存活率,同时延迟了芽的萌生和开花。所有对照品种从种植到开花的天数都很低,尽管与处理相比,对照品种的开花率很高。在经过100和150Gy处理的植物中观察到最高数量的枝条,而10、30和50Gy的伽马辐射显示出与所有对照品种相似的营养特征。在70、100和150Gy处理中观察到最大的叶片异常和叶片颜色变化。用10、30、50、70和150Gy处理的突变体产生了突变体品种,其中在30和70Gy处理中发现了更高数量的突变体和突变频率。结果表明,在所有品种中,由于较高的辐射暴露量和最小的营养生长变化,出现了更多的花色突变。这些结果建立了一个标准来确定可以诱导菊花突变的伽马辐射剂量。附加关键词:花色、生长参数、致死剂量、突变频率、茎切、营养生长
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引用次数: 1
Production of Grafted Vegetable Seedlings in the Republic of Korea: Achievements, Challenges and Perspectives 韩国嫁接蔬菜种苗生产的成就、挑战和展望
4区 农林科学 Q3 HORTICULTURE Pub Date : 2021-10-01 DOI: 10.7235/HORT.20210049
S. An, J. Bae, Ho Cheol Kim, Y. Kwack
Since the first commercial vegetable seedling production business was established in the Republic of Korea in 1992, technologies for the production of vegetable grafted seedlings have been developed. Commercial grafting nurseries have promoted the efficiency of grafted seedling production by the selection and concentration strategy. At present, most scions and rootstocks are concentrated on one or two cultivars and all grafted seedlings are made by the splice grafting method. To overcome the disadvantages of the splice grafting method, such as delayed formation of the grafted union and delayed rooting, light-emitting diode (LED) healing chambers (LHCs) have been developed for enhancing grafted union formation, and are currently used in 20% of commercial grafting nurseries. The commercial grafting nurseries in Korea have been facing highly difficult situations because of climate change, increased seedling production cost and labor shortage. To confront this challengeable circumstance, it is essential to optimize grafted seedling production practices to produce high-quality seedlings at lower cost and low impact on the environment, and to ensure a consistent supply to vegetable growers year round. Here, we explored several practices to improve productivity and reduce the environmental impact of grafted seedling production, including the development of disease resistant or abiotic stress tolerant rootstock cultivars, the development of more precise and efficient facilities and cultivation systems such as a grafting robot, the application of a plant factory with artificial lighting, the improvement of light condition in LHCs, and wastewater reuse. Additional key words: LED healing chamber (LHC), plug tray, rootstock, scion, splice grafting
自1992年大韩民国建立第一家商业蔬菜种苗生产企业以来,已经开发出了生产蔬菜嫁接苗的技术。商业嫁接苗圃通过选择和集中策略提高了嫁接苗的生产效率。目前,大多数接穗和砧木都集中在一个或两个品种上,所有的嫁接苗都是用剪接嫁接的方法制成的。为了克服拼接嫁接方法的缺点,如嫁接结合的延迟形成和生根延迟,已经开发了用于增强嫁接结合形成的发光二极管(LED)愈合室(LHCs),目前已在20%的商业嫁接苗圃中使用。由于气候变化、苗木生产成本增加和劳动力短缺,韩国的商业嫁接苗圃一直面临着高度困难的局面。为了应对这种具有挑战性的环境,必须优化嫁接苗的生产实践,以更低的成本和对环境的低影响生产出高质量的幼苗,并确保蔬菜种植者全年的稳定供应。在这里,我们探索了提高生产力和减少嫁接苗生产对环境影响的几种做法,包括开发抗病或非生物胁迫耐受的砧木品种,开发更精确高效的设施和栽培系统,如嫁接机器人,应用人工照明的植物工厂,LHCs中光照条件的改善以及废水的再利用。附加关键词:LED愈合室(LHC)、插盘、砧木、接穗、剪接嫁接
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引用次数: 5
Axillary Bud Development of ‘Shine Muscat’ Grapevine by Treatment of 6-benzylaminopurine, Spermidine, and Light 6-苄氨基嘌呤、亚精胺和光照处理‘亮肌’葡萄的腋芽发育
4区 农林科学 Q3 HORTICULTURE Pub Date : 2021-10-01 DOI: 10.7235/HORT.20210053
Bo Min Kim, Jun Young Park, M. Jung, Hee-Seung Park
‘Shine Muscat’ grape production is challenged by the frequent occurrence of empty branches that do not exhibit flowers on the shoot. Therefore, in this study, axillary bud development was investigated by treating with plant growth regulators [6-benzylaminopurine (BAP) and spermidine (SPD)] and an environmental factor (long-day treatment) during ecodormancy and the growth stage to reduce the occurrence of empty branches in ‘Shine Muscat’ grapevine. Treatments with growth regulators and light during ecodormancy did not affect bud size, but flower primordium formation and flower cluster appearance rates were the highest after BAP treatment. Flower primordium formation rate was the most efficient when 200 ppm BAP was applied after endodormancy break. When plants were treated with growth regulators in the growth stage, SPD treatment promoted the growth of shoots and buds but reduced the flower primordium formation rate. In contrast, BAP treatment inhibited bud growth but increased the flower primordium formation rate. In the case of long-day treatment, the formation of the flower primordium was also delayed as in an in vitro experiment. The whole bud necrosis rate was markedly low, and no significant difference was observed after all treatments. The flower primordium formation rates of the first and second buds that are mainly used for the cultivation of ‘Shine Muscat’ were remarkably low. Although BAP treatment promoted the flower primordium formation rate of the first and second buds, it was confirmed that the rate of cluster appearance in the following year was similar to the untreated group. Therefore, we suggest that 200 ppm BAP treatment during ecodormancy is the most efficient approach to reducing the occurrence of empty branches in ‘Shine Muscat’ grapevine. Additional key words: 6-benzylaminopurine, ecodormancy, empty branches, inflorescence primordium, short pruning
“Shine Muscat”葡萄的生产受到经常出现的枝条不开花的问题的挑战。因此,本研究通过植物生长调节剂[6-苄基氨基嘌呤(BAP)和亚精胺(SPD)]和环境因子(长日处理)在“亮麝香”葡萄的生态休眠和生长阶段处理腋芽发育,以减少空枝的发生。在生态休眠期间用生长调节剂和光照处理不影响芽的大小,但BAP处理后的花原基形成率和花簇出现率最高。花原基形成率在内胚层破坏后施用200ppm BAP时最有效。当植物在生长阶段用生长调节剂处理时,SPD处理促进了芽和芽的生长,但降低了花原基的形成率。相反,BAP处理抑制了芽的生长,但提高了花原基的形成率。在长日处理的情况下,花原基的形成也与体外实验一样延迟。全芽坏死率明显较低,各处理后无明显差异。主要用于“亮麝香”栽培的第一芽和第二芽的花原基形成率极低。虽然BAP处理促进了第一和第二芽的花原基形成率,但证实了第二年的簇出现率与未处理组相似。因此,我们建议在生态休眠期间用200ppm BAP处理是减少‘亮麝香’葡萄空枝发生的最有效方法。附加关键词:6-苄氨基嘌呤,生态休眠,空枝,花序原基,短修剪
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引用次数: 0
Potential Effects of Temperature Differences on the Soluble Sugar Content in Pear Fruit during the Growing Seasons of 2018 and 2019 2018年和2019年生长季节温差对梨果实可溶性糖含量的潜在影响
4区 农林科学 Q3 HORTICULTURE Pub Date : 2021-10-01 DOI: 10.7235/HORT.20210050
J. Cho, Ung Yang, S. Wi, Bok-Rye Lee, Se-Keung Oh, Minsoo Kim, Sang Hyun Lee
The impacts of climate change on crop yields and fruit quality are projected to accelerate with increased atmospheric carbon dioxide levels; however, few studies have focused on the impacts of climate change on the accumulation pattern and content of soluble sugars in pear (Pyrus pyrifolia) fruit. We compared the soluble sugars content and accumulation patterns during the 2018 and 2019 growing seasons throughout the developmental stages of pear fruit with climate data collected over the same period. Between the two years, we observed differences in the fructose and sucrose contents at the maturation stage of the pear fruit, resulting from differences in sugar accumulation following 132 days after full bloom (DAFB). Differences were also found in the meteorological data measured over the two years. In particular, the daily average temperatures from late-June to mid-August (73 to 132 DAFB) were all higher in 2018 than in 2019, and differences in the cumulative amounts of both fructose and sucrose were observed since 132 DAFB. Notable differences were confirmed in the comparison of the meteorological variables for each time interval. Among the meteorological variables, those related to temperature showed clear differences between the two years. Correlation coefficient matrices showed that sucrose and fructose accumulation responded differently depending on the meteorological variables over the two years. Furthermore, only accumulated temperature and air temperature were correlated with changes in the sucrose and fructose content in 2018, unlike in 2019. Taken together, our results indicate that temperature differences may have contributed to differences in the fructose and sucrose contents and their accumulation patterns over the two years. Additional key words: annual, comparisons, environmental, extreme, maturity, summer
气候变化对作物产量和水果质量的影响预计将随着大气二氧化碳水平的增加而加速;然而,很少有研究关注气候变化对梨果实可溶性糖积累模式和含量的影响。我们将2018年和2019年梨果实发育阶段生长季节的可溶性糖含量和积累模式与同期收集的气候数据进行了比较。在这两年之间,我们观察到梨果实成熟阶段果糖和蔗糖含量的差异,这是由于开花132天后糖积累的差异造成的。在这两年的气象数据中也发现了差异。特别是,2018年6月下旬至8月中旬(73至132 DAFB)的日均温度均高于2019年,自132 DAFB以来,果糖和蔗糖的累积量存在差异。在每个时间间隔的气象变量的比较中证实了显著的差异。在气象变量中,与温度相关的变量在两年之间表现出明显的差异。相关系数矩阵显示,蔗糖和果糖积累在两年内的反应因气象变量而异。此外,与2019年不同,2018年只有积温和气温与蔗糖和果糖含量的变化相关。总之,我们的研究结果表明,温度差异可能是果糖和蔗糖含量及其两年积累模式差异的原因之一。附加关键词:年度、比较、环境、极端、成熟、夏季
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引用次数: 0
Induction of Polyploidy in Cucumis melo ‘Chammel’ and Evaluation of Morphological and Cytogenetic Changes 甜瓜‘Chammel’多倍体的诱导及形态和细胞遗传学变化的评价
4区 农林科学 Q3 HORTICULTURE Pub Date : 2021-10-01 DOI: 10.7235/HORT.20210056
Woo-Young Cho, D. M. Deepo, Md. Mazharul Islam, Si-Chun Nam, Hong-Yul Kim, Jeungsul Han, Chang-kil Kim, M. Chung, K. Lim
This study explored the effects of oryzalin on the polyploidization of a new cultivar, Cucumis melo ‘Chammel’. 58 diploid seedlings of ‘Chammel’ were treated with oryzalin. Their ploidy levels were checked by flow cytometry and number of 5S and 18S rDNA loci were examined by fluorescence in situ hybridization (FISH). Twelve among 58 plants were identified as tetraploids. The tetraploids were self-crossed, and their seeds were harvested. The seeds and seedlings of the tetraploids were compared to the corresponding diploids using morphology and cytogenetic analyses. The seed width, the cotyledon width, and the leaf thickness of the tetraploids increased significantly over those of the diploids. However, the length of the petiole of the tetraploids was shorter than that of the corresponding diploids. The stomata length and width of the guard cells in the tetraploids were longer than in diploids, but the number of stomata per area decreased in the tetraploids. The number of chromosomes was 24 and 48 for the diploids and tetraploids, respectively. Moreover, the chromosomal changes induced by oryzalin were confirmed; the diploids had one pair of 5S rDNA loci and two pairs of 18S rDNA loci, the corresponding numbers were doubled in the tetraploids. These results will help to generate new seedless oriental melon cultivars via polyploidization. Additional key words: 5S rDNA, 18S rDNA, FISH, oryzalin, tetraploid
本研究探讨了稻谷苷对甜瓜新品种‘Chammel’多倍体化的影响。对58株‘Chammel’二倍体幼苗进行了oryzalin处理。流式细胞术检测其倍性水平,荧光原位杂交(FISH)检测其5S和18S rDNA位点数量。58株植物中有12株为四倍体。四倍体是自交的,它们的种子被收获了。将四倍体的种子和幼苗与相应的二倍体进行形态学和细胞遗传学分析。四倍体的种子宽度、子叶宽度和叶片厚度均显著高于二倍体。然而,四倍体的叶柄长度比相应的二倍体短。四倍体保护细胞的气孔长度和宽度都比二倍体长,但单位面积的气孔数量却减少了。二倍体和四倍体的染色体数分别为24条和48条。此外,还证实了oryzalin引起的染色体改变;二倍体有1对5S rDNA位点和2对18S rDNA位点,相应的数量在四倍体中增加一倍。这些结果将有助于通过多倍体分化培育无籽东方甜瓜新品种。附加关键词:5S rDNA, 18S rDNA, FISH, oryzalin,四倍体
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引用次数: 0
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Korean Journal of Horticultural Science & Technology
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