A method for measuring the anticoagulant activities of plasma low- and medium-molecular fractions is described. Use of this method permits differentiation between bleedings induced by high blood levels of these substances.
A method for measuring the anticoagulant activities of plasma low- and medium-molecular fractions is described. Use of this method permits differentiation between bleedings induced by high blood levels of these substances.
Analysis of a number of parameters of a lyophilized donor plasma reference sample to be used for the assessment of the prothrombin complex factors has shown the stability of this sample over the course of 14 months (a follow-up period). The sample may be used as a reference one in estimation of plasma prothrombin complex factor activities, and of PPSB activity. The reference sample activity should be estimated from the fresh donor plasma native pool, whose procoagulant activity is conventionally estimated as 100% or 1 U/ml. Use of a lyophilized reference sample helps obtain objective results compatible within this country, particularly so if all biologic reagents included in the test systems are standardized.
Cultivation characteristics of Shkolnikova's medium and medium No. 23 with human plasma or cattle blood serum added were studied by the radioactive indicator method. Cattle blood serum was preinactivated at 60 and 70 degrees C or intact. Plasma and serum preheated at 70 degrees C enhanced the growth of Mycobacterium tuberculosis. Intact serum or serum heated at lower temperature inhibited cell mitosis.
Hemolytic activities of Klebsiella-237 strains of various origins were measured in solid nutrient media by three methods making use of six different agar bases for the preparation of blood nutrient media. The authors analyze the agar bases, discuss the advantages and shortcomings of all the methods used with consideration for the purpose of the investigation. The findings evidence a higher hemolytic activity in Klebsiella isolated from patients as against the hemolytic activities of reference strains.
Blood antioxidant system parameters were examined in elderly subjects. The authors have developed methods for measurements of catalase, superoxide dismutase, and lipid peroxidation products. They introduce a new factor 'F' that is supposed to characterize the blood antioxidant system; this factor is based on the values of catalase and superoxide dismutase activities and the intensity of lipid peroxidation. The authors come to a conclusion that the blood antioxidant and oxidant systems may be more accurately described with the use of this new factor F. In case of an abdominal tumor whole blood catalase level is elevated and superoxide dismutase activity significantly reduced. Factor F values were found extremely low before death, therefore this factor may be considered an important criterion of a critical state. The blood antioxidant parameters of patients with diabetes mellitus and essential hypertension did not much differ from those of age-matched healthy subjects.
The authors suggest a method of functional probing of the complement alternative cascade, the jist of it being analysis of the neutrophil adhesion to sephadex granules treated with serum or another complement-containing substrate. The advantages of the method are analyzed, and the range of its application suggested.
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