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[Determination of the blood thiol disulfide balance using the coulometric titration method]. 库仑滴定法测定血中硫醇二硫平衡
Laboratornoe delo
Pub Date : 1991-01-01
S V Zits
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引用次数: 0
[Gamma-glutamyl transpeptidase: methodologic approaches to its determination and its diagnostic value (review of the literature)]. [γ -谷氨酰转肽酶:测定方法及其诊断价值(文献综述)]。
Laboratornoe delo
Pub Date : 1991-01-01
V N Titov, M G Tvorogova
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引用次数: 0
[The evaluation of lyophilized plasma as a reference standard for the prothrombin complex]. 【冻干血浆作为凝血酶原复合物参考标准物的评价】。
Laboratornoe delo
Pub Date : 1991-01-01
L N Tarasova, G K Platonova, E Iu Savinykh

Analysis of a number of parameters of a lyophilized donor plasma reference sample to be used for the assessment of the prothrombin complex factors has shown the stability of this sample over the course of 14 months (a follow-up period). The sample may be used as a reference one in estimation of plasma prothrombin complex factor activities, and of PPSB activity. The reference sample activity should be estimated from the fresh donor plasma native pool, whose procoagulant activity is conventionally estimated as 100% or 1 U/ml. Use of a lyophilized reference sample helps obtain objective results compatible within this country, particularly so if all biologic reagents included in the test systems are standardized.

对冻干供体血浆参考样品的一些参数进行分析，用于评估凝血酶原复合物因子，表明该样品在14个月(随访期间)的过程中具有稳定性。该样品可作为测定血浆凝血酶原复合因子活性和PPSB活性的参考样品。参考样本活性应从新鲜供体血浆原生池中估计，其促凝剂活性通常估计为100%或1 U/ml。使用冻干参比样品有助于获得在这个国家内兼容的客观结果，特别是如果测试系统中包含的所有生物试剂都是标准化的。
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引用次数: 0
[The use of Soviet antisera for determining immunoglobulins in the ICS-11 system (USA)]. [使用苏联抗血清测定ICS-11系统中的免疫球蛋白(美国)]。
Laboratornoe delo
Pub Date : 1991-01-01
N I Senishin, A V Petrukh
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引用次数: 0
[Tissue typing using a Soviet automated luminescent microscope-photometer]. [使用苏联自动发光显微光度计进行组织分型]。
Laboratornoe delo
Pub Date : 1991-01-01
E V Beliaeva, M I Zaraĭskiĭ, N P Timofeeva, L N Bubnova, S A Khomenkova, L V Solov'eva, S B Lopatkina, L D Serova

Automated fluorescent microscope developed by the authors permits photometry of microquantities of cellular suspensions in scanning standard 60-well plates with a flat bottom. Automated and semiautomated modes of operation are possible. Fluorescent stains and schemes of staining the examined cells that yield stable results in the lymphocytotoxic test have been selected. Comparative analysis of the efficacies of histocompatibility antigens detection by the routine and the fluorescent technique has shown a number of advantages of the fluorescent method. Specific features of fluorescent stains used for staining live cells are described.

作者开发的自动荧光显微镜允许在扫描标准60孔平板上对微量细胞悬浮液进行光度测定。自动化和半自动化的操作模式是可能的。选择了在淋巴细胞毒性试验中产生稳定结果的荧光染色和染色被检查细胞的方案。对常规法和荧光法检测组织相容性抗原的效果进行了比较分析，显示了荧光法的许多优点。描述了用于染色活细胞的荧光染色的具体特征。
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引用次数: 0
[The effect of human plasma and cattle blood serum on the culture properties of liquid nutrient media]. 人血浆和牛血清对液体营养培养基培养性能的影响。
Laboratornoe delo
Pub Date : 1991-01-01
E A Bibergal'

Cultivation characteristics of Shkolnikova's medium and medium No. 23 with human plasma or cattle blood serum added were studied by the radioactive indicator method. Cattle blood serum was preinactivated at 60 and 70 degrees C or intact. Plasma and serum preheated at 70 degrees C enhanced the growth of Mycobacterium tuberculosis. Intact serum or serum heated at lower temperature inhibited cell mitosis.

采用放射性指示剂法研究了添加人血浆或牛血清的施科尔尼科娃培养基和23号培养基的培养特性。牛血清在60℃和70℃预灭活或完整灭活。70℃预热的血浆和血清促进结核分枝杆菌的生长。完整血清或低温加热血清抑制细胞有丝分裂。
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引用次数: 0
[Apparatus for electrophoresis on cellulose acetate films]. [醋酸纤维素薄膜电泳装置]。
Laboratornoe delo
Pub Date : 1991-01-01
T I Cherkashina, V F Gruneva, E I Zorin, O V Troitskaia

Type EFPA-30 apparatus for electrophoresis on cellulose acetate films was developed at the Specialized Design Office for Biophysical Equipment of the Moscow Research and Production Association BIOFIZPRIBOR. Three autonomic separation cameras are united in one block, that permits separation of 12, 24, and 36 samples; the apparatus is supplied with time transducer, acoustic and visual indication. Clinical trials of the apparatus were carried out at the Institute of Biological and Medical Chemistry of the USSR Academy of Medical Sciences, its commercial production is to be started. The apparatus is intended for protein separation and may be used for studies of hemoglobins, serum proteins, lipoproteins, enzymes, and other biologic compounds. Methods for hemoglobin and serum protein separation are presented.

EFPA-30型醋酸纤维素薄膜电泳装置是由莫斯科研究和生产协会BIOFIZPRIBOR生物物理设备专门设计室开发的。三个自主分离相机统一在一个块中，允许分离12,24和36个样本;仪器配有时间传感器、声光显示。该仪器在苏联医学科学院生物和医学化学研究所进行了临床试验，即将开始商业化生产。该装置用于蛋白质分离，并且可用于血红蛋白、血清蛋白、脂蛋白、酶和其它生物化合物的研究。介绍了分离血红蛋白和血清蛋白的方法。
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引用次数: 0
[The advantages of using anti-A and anti-B TsOLI-clones for determining blood groups]. [使用抗a和抗b tsoli克隆测定血型的优势]。
Laboratornoe delo
Pub Date : 1991-01-01
L A Kharchenko, V F Iakshov, O N Bil'
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引用次数: 0
[Determination of the monoamine oxidase and the diamine oxidase activity of a single blood sample]. [单血单胺氧化酶和二胺氧化酶活性的测定]。
Laboratornoe delo
Pub Date : 1991-01-01
G A Sivoraksha, Iu N Sidel'nikov

The known methods for simultaneous measurements of blood histamine and serotonin have prompted the authors to search for approaches to simultaneous measurements of monoamine oxidase and diamine oxidase, the enzymes contributing to serotonin and histamine inactivation. The authors suggest a method for simultaneous measurements of the blood serum monoamine oxidase and diamine oxidase activities that permits cut down the time of the blood and substrate incubation to 10 min, essentially shortens the procedure and simplifies it. The method is recommended for research and practice.

同时测量血液组胺和血清素的已知方法促使作者寻找同时测量单胺氧化酶和二胺氧化酶的方法，这两种酶有助于血清素和组胺失活。作者提出了一种同时测量血清单胺氧化酶和二胺氧化酶活性的方法，可以将血液和底物的孵育时间减少到10分钟，从根本上缩短了程序并简化了它。该方法具有一定的研究和实践价值。
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引用次数: 0
[A method of detecting phages in lysogenic strains of Vibrio cholerae]. 一种检测霍乱弧菌溶原菌株中噬菌体的方法。
Laboratornoe delo
Pub Date : 1991-01-01
T A Kudriakova, L D Makedonova, L R Cherkasova, O V Dudkina
{"title":"[A method of detecting phages in lysogenic strains of Vibrio cholerae].","authors":"T A Kudriakova,&nbsp;L D Makedonova,&nbsp;L R Cherkasova,&nbsp;O V Dudkina","doi":"","DOIUrl":"","url":null,"abstract":"","PeriodicalId":18012,"journal":{"name":"Laboratornoe delo","volume":" 2","pages":"56-7"},"PeriodicalIF":0.0,"publicationDate":"1991-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"12874071","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
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