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Quantification of Bioactive Compounds in Piper Betle Leaf Extract by Gas Chromatography-Mass Spectrometry (GC-MS) 气相色谱-质谱联用(GC-MS)定量分析槟榔叶提取物中生物活性成分
Pub Date : 2022-01-01 DOI: 10.29321/maj.10.000650
Sathiamoorthy V, G. P., B. M.
Piper betleis a Piperaceae family scented perennial creeper. The leaves are high in phenol, a compound with anti-tumor, anti-mutagenic, and immunomodulatory activities. This study aims to evaluate the bioactive compound in Piper betle leaves extract using Gas Chromatography Mass Spectrometry (GC-MS). Over 100 compounds were found in the GC-MS results, with 19 of them having a probability of greater than 30. The compound phentermine shows the highest peak at a retention time of 9.346 minutes, followed by Hexadecanoic acid, Tetradecanoic acid, Eugenol, Dodecanoic acid. Hexadecanoic acid shows the highest area% about 26.665%, indicating the highest composition of hexadecanoic acid in the betel leaf extract. The result revealed that the compound in betel leaf extract possesses medicinal properties.
腊梅是腊梅科多年生有香味的爬山虎植物。其叶子富含酚,酚是一种具有抗肿瘤、抗诱变和免疫调节活性的化合物。本研究旨在利用气相色谱-质谱联用技术(GC-MS)对花椒叶提取物中的生物活性成分进行评价。在GC-MS结果中发现了100多个化合物,其中19个化合物的概率大于30。芬特明的保留时间最长,为9.346 min,其次为十六酸、十四酸、丁香酚、十二酸。槟榔叶提取物中棕榈酸的面积%最高,约为26.665%,说明槟榔叶提取物中棕榈酸的含量最高。结果表明,槟榔叶提取物中的化合物具有一定的药用价值。
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引用次数: 0
Study on Isolation, Effectiveness and Formulation of Native Trichoderma spp. from Jhum, Virgin and Cultivated Land of Nagaland 那加兰邦原生、原生和耕地木霉的分离、药效及配方研究
Pub Date : 2022-01-01 DOI: 10.29321/maj.10.000703
Chavan Digvijay Rajaram, S. Banik
Ten Trichoderma isolates named as Trichoderma isolate1 to Trichoderma isolate 10, were isolated from different soil samples collected from virgin, Jhum, cultivated, and fallow land of Medziphema block of Nagaland state by serial dilution agar plate method on Trichoderma selective medium (TSM). All the Trichoderma isolates were tested against Fusarium solani and Sclerotium rolfsii by dual culture method. All the isolates were highly effective against both the pathogens with 83-93 percent and 72-89 per cent growth inhibition of Fusarium solani and Sclerotium rolfsii, respectively. Among the tested Trichoderma isolates, Trichoderma isolate1 was observed most effective; therefore, it was mass multiplied in Potato Dextrose Broth and formulated in talc powder. The colony forming units were counted thrice to determine the number of viable conidia in product; the first counting was done on the same day of formulation, the second counting was done one month, and the last was done six months after formulation. It was observed that the colony forming units decreased from 13 × 106 c.f.u. to 5.66 × 106 c.f.u. after six months of storage from the initial formulation which indicates that even after six months of storage, the Trichoderma isolate 1 formulated by using talc powder still contained good number of viable conidia. Thus, from the overall results obtained from the present work, it can be concluded that Trichoderma spp. as a ubiquitous fungus and an effective biological control agent can be one of the paramount components of integrated disease management.
以木霉选择培养基(Trichoderma selective medium, TSM)为培养基,采用琼脂平板连续稀释法,从印度那加兰邦Medziphema区块的原始、Jhum、耕地和休耕地不同土壤样品中分离出10株木霉,分别命名为Trichoderma isolate1至Trichoderma isolate 10。采用双重培养法对分离的木霉进行了番茄枯萎菌和罗尔夫菌核菌的抑菌试验。所有分离菌株对茄枯菌和罗氏菌核菌的生长抑制率分别为83% ~ 93%和72% ~ 89%。实验分离的木霉菌株中,分离木霉1效果最好;因此,在马铃薯葡萄糖肉汤中大量繁殖,并在滑石粉中配制。对菌落形成单位进行三次计数,以确定产物中有活孢子的数量;第一次计数在配药当天进行,第二次计数在配药一个月后进行,最后一次计数在配药六个月后进行。结果表明,经过6个月的发酵,原配方的菌落形成单位由13 × 106 c.f.u.下降到5.66 × 106 c.f.u.,说明滑石粉配方的木霉分离物1在发酵6个月后仍含有大量的活孢子。因此,从本研究获得的总体结果可以得出结论,木霉作为一种普遍存在的真菌和有效的生物防治剂,可以成为综合疾病管理的重要组成部分之一。
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引用次数: 0
Financial Inclusion through Self Help Groups – An Economic Catalyst to transform Rural India 通过自助团体实现金融包容性——改变印度农村的经济催化剂
Pub Date : 2022-01-01 DOI: 10.29321/maj.10.000713
Kalaiarasi D, R. A
This study attempted to create a comprehensive measure of Financial Inclusion (FI) with specific focus on SHGs among 33 major states in India from the period of 2016 to 2020. Principal Component Analysis was employed to track the status of inclusion in Indian financial sector. The link between FI and development indicators like agricultural and industrial growth, access to basic education was explored through Ordered Probit Random effect model using panel data. Results indicated that majority of Eastern and North Eastern states have poor FI, in addition Financial Inclusion Index (FII) showed a positive relation with agricultural growth
本研究试图创建一个全面的金融包容性(FI)衡量标准,特别关注2016年至2020年期间印度33个主要州的shg。采用主成分分析来跟踪印度金融部门的包容性状况。通过使用面板数据的有序Probit随机效应模型,探讨金融融资与农业和工业增长、基础教育普及等发展指标之间的联系。结果表明,东部和东北部大多数邦的金融包容性指数较差,金融包容性指数(FII)与农业增长呈正相关
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引用次数: 0
Content Analysis of Agriculture News Published in English Daily (Business Line) 《英语日报》(业务线)农业新闻内容分析
Pub Date : 2022-01-01 DOI: 10.29321/maj.10.000591
A. M, K. C., S. M
Content analysis can be used with various data sources, including textual data, visual stimuli (e.g., photographs/videos), and audio data. An English daily newspaper (Business Line) was purposively selected for content analysis study. From June 2020 to May 2021, the daily newspapers were surveyed to study the subject matter coverage and monthly frequency. The study shows that the news published was highly about government policies/plans/reforms, weather & climate, commodities sales & trends, and exports and imports. From the selected year, it was found that highly frequent coverage of the agricultural news was observed during October, followed by June and May months. Readability analysis was done by randomly selecting five percent of the articles from the total news items identified. From the analysis, it can be viewed that the level of difficulty in reading the articles ranged from standard to very difficult. The average score was found as 46.9, which is “Difficult” to read. It is concluded that the newspaper had good coverage of agriculture articles and it has been suggested to reduce the difficulty level of the contents of the articles.
内容分析可用于各种数据源,包括文本数据、视觉刺激(例如,照片/视频)和音频数据。有意选择一份英文日报(Business Line)进行内容分析研究。从2020年6月至2021年5月,对日报进行调查,研究主题覆盖率和月度频率。研究表明,发布的新闻高度关注政府政策/计划/改革、天气和气候、商品销售和趋势以及进出口。从所选年份来看,农业新闻报道频率最高的月份是10月份,其次是6月和5月。可读性分析是通过从所有确定的新闻项目中随机选择5%的文章来完成的。从分析中可以看出,阅读文章的难度程度从一般到非常困难。平均分数为46.9分,属于“难读”。结果表明,该报农业类文章报道较好,建议降低文章内容的难度。
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引用次数: 0
Estimation of Water Use Efficiency and Osmolyte accumulation to Evaluate Drought Stress Tolerance in Mulberry 桑树水分利用效率和渗透物积累评价抗旱性的研究
Pub Date : 2022-01-01 DOI: 10.29321/maj.10.000662
D. R, V. D
The present study was under taken to assess the drought stress tolerance in four mulberry genotypes and three varieties. Four months old mulberry plants were subjected to three water regimes viz., 100% PC, 50% PC, 25% PC for 30 days. Chlorophyll Stability Index, intrinsic Water Use Efficiency and osmolyte accumulation (proline content) were estimated before imposing drought stress and 30 days after drought stress. WUEi and proline accumulation was increased, while CSI, carbohydrate and protein contents were found to be decreased as severity of drought stress progressed. Mulberry genotype, MI-0425 was found to be drought tolerant with higher WUEi (4.13 mmol CO2 mol-1 H2O) and proline accumulation (8.54 μg g-1). This line also showed lesser protein degradation at severe drought stress. The genotype MI-0613 recorded lower WUEi (3.18 mmol CO2 mol-1 H2O) and proline content (5.87 μg g-1) under severe drought stress. It also recorded severe reduction in yield (45.96%) under stress. Hence, MI-0613 was identified as a drought susceptible genotype. Variety V1 recorded higher CSI (77.68%) and carbohydrate (29.03 mg/g) and yield (95.48 g/plant) under both in moderate and intense water stress treatments. Hence, V1 was found to mitigate drought stress by maintaining higher CSI and carbohydrate content.
对桑树4个基因型和3个品种的抗旱性进行了研究。4个月大的桑树植株分别施用100% PC、50% PC、25% PC三种水处理30天。测定了干旱胁迫前和干旱胁迫后30 d叶绿素稳定指数、内在水分利用效率和渗透物积累量(脯氨酸含量)。随着干旱胁迫程度的加重,WUEi和脯氨酸积累增加,而CSI、碳水化合物和蛋白质含量降低。桑基因型MI-0425具有较高的WUEi (4.13 mmol CO2 mol-1 H2O)和脯氨酸积累(8.54 μg -1)的耐旱性。该品系在严重干旱胁迫下也表现出较少的蛋白质降解。基因型MI-0613在严重干旱胁迫下WUEi (3.18 mmol CO2 mol-1 H2O)和脯氨酸含量(5.87 μg -1)较低。在胁迫下,其产量也出现了严重下降(45.96%)。因此,MI-0613被确定为干旱易感基因型。在中度和重度水分胁迫下,V1品种CSI(77.68%)、碳水化合物(29.03 mg/g)和产量(95.48 g/株)均较高。因此,V1通过维持较高的CSI和碳水化合物含量来缓解干旱胁迫。
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引用次数: 0
Parasitic Potential of Bracon brevicornis Wesmael (Hymenoptera: Braconidae) on Spodoptera frugiperda (J. E. Smith, 1797) (Lepidoptera: Noctuidae) 短翅小茧蜂(膜翅目:小茧蜂科)对夜蛾的寄生潜力(J. E. Smith, 1797)
Pub Date : 2022-01-01 DOI: 10.29321/maj.10.000676
Lekha priyanka S, Jeyarani S, S. N, M. S.
Maize is one of the most important cereal crops and is called ‘the queen of cereals’ because of its high yielding potential than other cereals. Fall armyworm, Spodoptera frugiperda is one of the serious invasive pests found to cause higher yield loss and extensive damage on maize. Considering the ecological and environmental consequences, biological control of insect pests is gaining importance as an alternative to insecticides. However, the success of any biological control programme depends on the appropriate parasitoid: host ratio and the biological stage of the pest susceptible to parasitoid. To fortify the above facts, the present study was aimed to determine the parasitic potential of B. brevicornis on S. frugiperda through free and no choice tests. This study indicated highest parasitization at the parasitoid: host ratio of 5:1 with 68.60 and 64.95 per cent parasitization on the VI instar followed by V instar (57.75 and 56.95 %), IV instar (48.80 and 46.60 %) and III instar (32.40 and 30.67 %) respectively under free and no choice conditions, respectively. Among the parasitoid host ratios tested 5: 1 was found to be the best indicating that the parasitic potential of B. brevicornis was directly proportional to the parasitoid densities. Biological parameters of B. brevicornis studied on different S. frugiperda instars recorded highest number of eggs (32.00), grub (30.75), cocoon (29.75) and adults (28.00) on sixth instar larva of S. frugiperda followed by fifth instar larva of S. frugiperda. The results revealed that the larval size was directly proportional to the progeny development of B. brevicornis. The egg, grub and cocoon period of B. brevicornis on S. frugiperda ranged from 24.20 to 26.20 h, 72.26 to 73.20 h, 96.20 to 98.20 h, respectively. The total life cycle was completed in 192.66 to 195.80 h in all instars and was found to be on par with all the instars studied. The longevity of adults emerged from different instars were found to be on par with each other and it ranged from 362.0 to 362.8 h.
玉米是最重要的谷类作物之一,因其比其他谷物具有更高的产量潜力而被称为“谷物女王”。秋粘虫(Spodoptera frugiperda)是危害玉米产量损失大、危害广泛的严重入侵害虫之一。考虑到害虫对生态和环境的影响,生物防治作为一种杀虫剂的替代品正变得越来越重要。然而,任何生物防治方案的成功取决于适当的拟寄主比例和易受拟寄主影响的害虫的生物学阶段。为了证实上述事实,本研究旨在通过自由试验和无选择试验确定短角贝螨对frugiperda的寄生潜力。结果表明,在无选择和无选择条件下,寄生率最高的寄生蜂为5:1,寄生率为68.60%和64.95%,其次为V龄(57.75%和56.95%)、IV龄(48.80%和46.60%)和III龄(32.40%和30.67%)。以5:1的寄主比例为最佳,说明短喙小蠊的寄生潜力与寄主密度成正比。在不同龄期对短角蠓的生物学参数进行了研究,结果显示,短角蠓卵(32.00)、蛴螬(30.75)、茧(29.75)、成虫(28.00)在6龄幼虫上最多,5龄幼虫次之。结果表明,短喙贝的幼虫大小与后代发育成正比。短角布氏螨对frugiperda的卵期为24.20 ~ 26.20 h,蛴螬期为72.26 ~ 73.20 h,茧期为96.20 ~ 98.20 h。所有龄期的总生命周期为192.66 ~ 195.80 h,与所研究的所有龄期基本一致。不同龄期的成虫寿命在362.0 ~ 362.8 h之间,基本一致。
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引用次数: 1
Assessment of Influential Factors for Farmers towards Arecanut (Areca catechu) Cultivation in Tamil Nadu 泰米尔纳德邦农民种植槟榔的影响因素评价
Pub Date : 2022-01-01 DOI: 10.29321/maj.10.000643
M. V., V. R, P. K., B. A
In India, one of the most important cash crops is Arecanut (Areca catechu), which is referred to as a traditional medicine in Indian Ayurveda texts. India ranks first in area and production of arecanut, accounting for 54.07 per cent of its world production. In Tamil Nadu arecanut is cultivated in 6,884 ha of area with 35% area is under Salem district with a sample size of 120 arecanut farmers was selected by proportionate random sampling technique. Kaiser Meyer Olkin Measures the sampling adequacy, for the present study, the value was 0.717, which signifies the sample is adequate and middling as used by most scientists. Bartlett’s test of sphericity is 676.687, while the significance is 0.000,which implies the variables are adequate for the study. Five factors were extracted from the sixteen components with Eigen values and factor loadings witha total variance of 64.95 per cent. The functional factor had high variance of 18.32% with seven-factor loadings, cognizance factors with 15.30% variance with six-factor loadings followed by empirical factor with 15.12% variance for three-factor loadings, household factor having variance of 8.48% with two-factor loadings and decisive factor having variance of 7.71% with three-factor loadings. This study suggests promoting improved methods of cultivation at the farm level to increase household income and for expansion of arecanut areas.
在印度,最重要的经济作物之一是槟榔(槟榔),在印度阿育吠陀文献中被称为传统药物。印度在槟榔的面积和产量方面排名第一,占世界槟榔产量的54.07%。在泰米尔纳德邦,种植槟榔的面积为6,884公顷,其中35%的面积在Salem地区,通过比例随机抽样技术选择了120名槟榔种植者。Kaiser Meyer Olkin测量样本充分性,对于本研究,该值为0.717,这意味着大多数科学家使用的样本是充足的和中等的。Bartlett的球形度检验为676.687,显著性为0.000,说明变量适合本研究。从具有特征值和因子负荷的16个成分中提取出5个因子,总方差为64.95%,其中功能因子在7因素负荷下方差高达18.32%,认知因子在6因素负荷下方差高达15.30%,经验因子在3因素负荷下方差高达15.12%,家庭因子在2因素负荷下方差高达8.48%,决定性因子在3因素负荷下方差高达7.71%。本研究建议在农场一级推广改良的种植方法,以增加家庭收入和扩大槟榔种植面积。
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引用次数: 0
Energy Densification of Groundnut Shell through Microwave-Assisted Hydrothermal Carbonization 微波辅助水热碳化花生壳的能量致密化研究
Pub Date : 2022-01-01 DOI: 10.29321/maj.10.000639
K. P, S. P
Modern technology of microwave synthesis was utilized for hydrochar production from groundnut shell biomass. Hydrochar was produced at three different temperatures (180, 200, and 220°C) with 20 minutes of holding time and analyzed for the physicochemical characteristics. The yield of hydrochar and hydrocrude were in the range of 45 to 61 % and 51 to 56 % respectively. The maximum value of the higher heating value of 19.11 MJ/kg was obtained for hydrochar produced at 220°C, which is similar to the higher heating value of lignite (19 MJ/kg). The energy densification ratio of respective hydrochar was 1.13 with 51.77 % energy yield. The result implied that microwave-assisted hydrothermal carbonization was an energyefficient method to produce hydrochar. Hydrochar produced from groundnut shells was an eco-friendly, energy enriched product that can be used as a solid fuel.
利用现代微波合成技术,对花生壳生物质进行了制烃研究。在三种不同的温度下(180、200和220℃),保温时间为20分钟,生成碳氢化合物,并分析其物理化学特性。烃类和原油的收率分别为45% ~ 61%和51% ~ 56%。220℃下生成的烃类的较高热值最大值为19.11 MJ/kg,与褐煤的较高热值(19 MJ/kg)相似。各馏分的能量密度比为1.13,产能率为51.77%。结果表明,微波辅助水热碳化是一种高效的制备烃类的方法。从花生壳中产生的碳氢化合物是一种环保的、能量丰富的产品,可以用作固体燃料。
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引用次数: 0
Determination of Physical Properties of Cotton Plant in the Development of Cotton Uprooter cum Shredder 棉花连根拔碎机研制过程中棉花植株物理特性的测定
Pub Date : 2022-01-01 DOI: 10.29321/maj.10.000690
Raju Yadav K, Reddy Sj, A. A, M. K
The cotton in India provides direct livelihood to 6 million farmers and about 40-50 million people are employed in the cotton trade and its processing in India. Cotton is a tropical and subtropical crop. For successful germination of its seeds, a minimum temperature of 15ºC is required. It is grown mainly as a dry crop in black cotton and medium black soil. The physical properties of the cotton plant are very important to optimize the design parameters of cottonuprooter cum shredder. Physical properties such asstalk stem diameter, plant height, and taproot depth were determined for the development of cotton uprooter cum shredder. The obtained results for stalk stem diameter, plant height, and taproot depth were2.2 cm,117.4 cm, and 32.7 cm, respectively. The moisture content of the cotton stalks is 27.3% and the soil moisture content was found to be 11.1%. The maximum deviation of the cotton plant was found to be 7.5 cm.
印度的棉花为600万农民提供了直接生计,印度约有4000万至5000万人从事棉花贸易及其加工。棉花是热带和亚热带作物。为了使其种子成功发芽,最低温度需要15℃。它主要作为一种干性作物种植在黑色棉花和中等黑色土壤上。棉花植株的物理特性对拔棉碎纸机设计参数的优化至关重要。测定了棉花根茎直径、株高、主根深度等物理特性,为棉花除根碎纸机的研制奠定了基础。茎粗、株高和主根深分别为2.2 cm、117.4 cm和32.7 cm。棉花秸秆含水率为27.3%,土壤含水率为11.1%。棉花植株的最大偏差为7.5 cm。
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引用次数: 0
Potentiality of Novel Chitosan-O-Arginine as Insecticidal and Growth Inhibitory Compound against Diamondback Moth, Plutella xylostella L. on Cauliflower 新型壳聚糖- o -精氨酸作为菜花小菜蛾杀虫抑生化合物的潜力
Pub Date : 2022-01-01 DOI: 10.29321/maj.10.000588
S. S., S. M., M. M., V. S., H. S, S. K
Chitosan (Cs), a natural biodegradable polysaccharide polymer, prepared from sea waste of crustacean origin, has the potential for plant protection application. In the present study, a novel chitosan derivative, called Chitosan-O-Arginine, synthesized from crude chitosan, was evaluated for its chronic toxicity against second instar larvae of Plutella xylostella by leaf dip method. The results revealed the toxic potential of Chitosan-O-Arginine, which could inflict cent per cent mortality after four days of continuous feeding on the 1000 ppm treated leaf, and the larva could accomplish 0.12 mg weight (99.66% reduction) on the day before death. The next lower concentration (700 ppm) resulted in the maximum larval weight of 0.45 mg (93.71% reduction) after nine days of feeding and resulted in cent per cent mortality after the tenth day. While the untreated larva attained a maximum weight of 5.54 mg. All the larvae were pupated in the next lower concentration (500 ppm) and recorded minimum pupal (1.00 mg; 80% reduction) and adult (0.73 mg; 77.45% reduction) weight, whereas the untreated pupa and adult weighed the maximum of 5.00 mg and 3.20 mg. Considering the developmental period, there was no significant difference in the larval duration and adult life span. While there was an extension of the pupal period by one day at 500 ppm compared to untreated larva. The larval, pupal, and adult malformations were noticed in 300 and 100 ppm concentrations. These findings suggest that chitosan was potent in imposing chronic toxicity and growth inhibitory effect on P. xylostella larvae, and hence it can be recommended as an eco-friendly component in the Integrated Pest Management module.
壳聚糖(Cs)是一种天然的可生物降解的多糖聚合物,由海洋甲壳类废弃物制备而成,具有潜在的植物保护应用前景。本研究以壳聚糖为原料合成一种新型壳聚糖衍生物壳聚糖- o -精氨酸,并采用叶浸法研究其对小菜蛾二龄幼虫的慢性毒性。结果显示,壳聚糖- o -精氨酸的毒性潜力,在1000 ppm处理的叶片上连续取食4天后,其死亡率可达到百分之百,幼虫在死亡前一天可达到0.12 mg(99.66%)。下一个较低浓度(700 ppm)在饲养9天后,幼虫最大体重为0.45 mg(减少93.71%),第10天后死亡率为100%。而未经处理的幼虫最大体重为5.54 mg。所有幼虫均在较低浓度(500 ppm)下化蛹,记录最低化蛹量(1.00 mg;减少80%)和成人(0.73毫克;而未经处理的蛹和成虫的最大体重分别为5.00 mg和3.20 mg。从发育期来看,幼虫期和成虫寿命差异不显著。而与未经处理的幼虫相比,在500ppm的浓度下蛹期延长了一天。在300和100 ppm浓度下,幼虫、蛹和成虫均出现畸形。综上所述,壳聚糖对小菜蛾幼虫具有较强的慢性毒性和生长抑制作用,可作为生态友好型有害生物综合防治模块的组成部分。
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引用次数: 0
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Madras Agricultural Journal
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