Pub Date : 2023-07-01DOI: 10.55230/mabjournal.v52i2.2510
Muhammad Arif Samshuri, C. Safuan, N. A. Maznan, S. Jaafar, Z. Bachok
A healthy coral reef is linked to the mutualistic relationship between scleractinian coral and the symbiotic Symbiodiniacea (SD). However, there is limited research on SD in Malaysia, despite its important role in reef-building coral. In this study, the SD density of scleractinian corals from the family Acroporidae was evaluated to (i) examine the pattern of SD density and (ii) comprehend the regulation of the SD density by the host. The mean SD density ranged between 0.46 ± 0.01 × 106 cell cm-2 and 2.98 ± 1.17 × 106 cell cm-2. It is hypothetically proven that the SD density differed significantly between genera and morphological factors such as colony surface area (CSA) and dry weight coral tissue per unit colony surface area (DWCT/CSA) were significantly correlated with the SD density. The results show that the significant variation in SD density among coral genera can be influenced by coral growth forms and tissue biomass. There was a significant relationship between SD density and CSA as well as DWCT/CSA. Coral genera with a wider CSA and lower DWCT/CSA such as Anacropora and Acropora with branching, digitate and tabulate growth forms contained lower SD density than massive, laminar, and encrusting such as Montipora and Astreopora which hold more DWCT/CSA at lower CSA, resulting in higher SD density. The findings provide valuable information on SD density in different types of corals from the southern part of the South China Sea and reveal the coral host’s SD regulation.
{"title":"Symbiodiniaceae Density Pattern in Relation To Colony Morphology of Scleractinian Corals in Pulau Tioman and Pulau Bidong, Malaysia","authors":"Muhammad Arif Samshuri, C. Safuan, N. A. Maznan, S. Jaafar, Z. Bachok","doi":"10.55230/mabjournal.v52i2.2510","DOIUrl":"https://doi.org/10.55230/mabjournal.v52i2.2510","url":null,"abstract":"A healthy coral reef is linked to the mutualistic relationship between scleractinian coral and the symbiotic Symbiodiniacea (SD). However, there is limited research on SD in Malaysia, despite its important role in reef-building coral. In this study, the SD density of scleractinian corals from the family Acroporidae was evaluated to (i) examine the pattern of SD density and (ii) comprehend the regulation of the SD density by the host. The mean SD density ranged between 0.46 ± 0.01 × 106 cell cm-2 and 2.98 ± 1.17 × 106 cell cm-2. It is hypothetically proven that the SD density differed significantly between genera and morphological factors such as colony surface area (CSA) and dry weight coral tissue per unit colony surface area (DWCT/CSA) were significantly correlated with the SD density. The results show that the significant variation in SD density among coral genera can be influenced by coral growth forms and tissue biomass. There was a significant relationship between SD density and CSA as well as DWCT/CSA. Coral genera with a wider CSA and lower DWCT/CSA such as Anacropora and Acropora with branching, digitate and tabulate growth forms contained lower SD density than massive, laminar, and encrusting such as Montipora and Astreopora which hold more DWCT/CSA at lower CSA, resulting in higher SD density. The findings provide valuable information on SD density in different types of corals from the southern part of the South China Sea and reveal the coral host’s SD regulation.","PeriodicalId":18160,"journal":{"name":"Malaysian applied biology","volume":"28 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"77705788","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-06-30DOI: 10.55230/mabjournal.v52i2.2617
A. Dasuki, Y. Esa, A. Christianus, Mohammed Fadhil Syukri Ismail
Rapid genetic improvements from selective breeding are anticipated in many aquaculture species and mitogenome is used to complement the morphological taxonomy of hybrids to evaluate its genetic structures. A study of the genetic variations within the two strains (Malaysian strain and Golden Thai strain) of Anabas testudineus (Bloch, 1792) and their hybrids, phylogenetic trees based on cytochrome oxidase subunit 1 (CO1) of the partial mitochondrial DNA gene were constructed using Maximum Likelihood and Neighbor Joining approaches. The findings support the monophyletic status of the genus with only one haplogroup from which other haplotypes were descended and of a single common ancestor. The individual fishes’ phylogenetic relationships revealed two major clades and Saurida undosquamis as an outgroup. All the groups had high haplotype diversity, except for the hybrid Malaysian strain × golden Thai strain (0.1540±0.126). This suggests that different fish species in each of these studies had different nucleotide compositions in their mitochondrial genomes. The highest number of haplotypes and the presence of distinct haplotypes in the Golden Thai strain × Malaysian strain hybrid point to the absence of recent or regular gene flow as well as high genetic diversity within the hybrids. This study demonstrated that the mtDNA diversity of Anabas testudineus from Malaysia and Thailand had been preserved. Studies on the population genetic diversity and molecular evolution of anabas fish species can benefit from the data provided by this work.
{"title":"Genetic Variations of Malaysian And Golden Thai Strains of Climbing Perch and Their Hybrids Based On The Partial Mitochondrial Cytochrome Oxidase Subunit 1 Gene","authors":"A. Dasuki, Y. Esa, A. Christianus, Mohammed Fadhil Syukri Ismail","doi":"10.55230/mabjournal.v52i2.2617","DOIUrl":"https://doi.org/10.55230/mabjournal.v52i2.2617","url":null,"abstract":"Rapid genetic improvements from selective breeding are anticipated in many aquaculture species and mitogenome is used to complement the morphological taxonomy of hybrids to evaluate its genetic structures. A study of the genetic variations within the two strains (Malaysian strain and Golden Thai strain) of Anabas testudineus (Bloch, 1792) and their hybrids, phylogenetic trees based on cytochrome oxidase subunit 1 (CO1) of the partial mitochondrial DNA gene were constructed using Maximum Likelihood and Neighbor Joining approaches. The findings support the monophyletic status of the genus with only one haplogroup from which other haplotypes were descended and of a single common ancestor. The individual fishes’ phylogenetic relationships revealed two major clades and Saurida undosquamis as an outgroup. All the groups had high haplotype diversity, except for the hybrid Malaysian strain × golden Thai strain (0.1540±0.126). This suggests that different fish species in each of these studies had different nucleotide compositions in their mitochondrial genomes. The highest number of haplotypes and the presence of distinct haplotypes in the Golden Thai strain × Malaysian strain hybrid point to the absence of recent or regular gene flow as well as high genetic diversity within the hybrids. This study demonstrated that the mtDNA diversity of Anabas testudineus from Malaysia and Thailand had been preserved. Studies on the population genetic diversity and molecular evolution of anabas fish species can benefit from the data provided by this work.","PeriodicalId":18160,"journal":{"name":"Malaysian applied biology","volume":"77 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-06-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"74842484","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-06-30DOI: 10.55230/mabjournal.v52i2.2599
Y. V. Saraswati, F. Mustofa, A. P. Z. N. L. Sari, H. Sasongko, B. Ariyadi, D. Maharani
The uniformity of live weight in the early stage (DOC) is an indicator applied to evaluate the quality of brooding, raising, and growth management. This study aims to compare the live weight uniformity of DOC in three local breeds as a parental group, namely Merawang A and B (n:196), Murung Panggang (n:157), and KUB (n:416), as well as in five groups of Bulaksumur (BS) for crossbred group, namely BS-1 (n:136), BS-2 (n:76), BS-3 (n:106), BS-5 (n:81), BS-6 (n:164). The live weight data of DOC was obtained in Gunungkidul Regency under similar management. The results show that the BS groups had higher live weight (31.9 g ± 3.1) compared to Merawang A (24 g ± 3.1), Murung Panggang (28.2 g ± 2.3), and KUB (28.1 g ± 2.8). Furthermore, the live weight uniformity on the BS DOC averaged 71% across all groups, with the highest uniformity being BS-3 (87%). Murung Panggang had a higher live weight uniformity (82%) compared to Merawang A and B (42%, 64%) and KUB (63%). The average coefficient variance was less than 10%, except for Merawang A and BS-2 with 13.09% and 11.35%, respectively. In conclusion, the DOC of the crossbred (BS) was more uniform than its parental groups. However, to distinguish the significantly different in the chicken live weight in early stage, further analysis needs to be conducted in the future.
{"title":"Live Weight Uniformity on The Day-Old Chick of Indonesian Local Chickens","authors":"Y. V. Saraswati, F. Mustofa, A. P. Z. N. L. Sari, H. Sasongko, B. Ariyadi, D. Maharani","doi":"10.55230/mabjournal.v52i2.2599","DOIUrl":"https://doi.org/10.55230/mabjournal.v52i2.2599","url":null,"abstract":"The uniformity of live weight in the early stage (DOC) is an indicator applied to evaluate the quality of brooding, raising, and growth management. This study aims to compare the live weight uniformity of DOC in three local breeds as a parental group, namely Merawang A and B (n:196), Murung Panggang (n:157), and KUB (n:416), as well as in five groups of Bulaksumur (BS) for crossbred group, namely BS-1 (n:136), BS-2 (n:76), BS-3 (n:106), BS-5 (n:81), BS-6 (n:164). The live weight data of DOC was obtained in Gunungkidul Regency under similar management. The results show that the BS groups had higher live weight (31.9 g ± 3.1) compared to Merawang A (24 g ± 3.1), Murung Panggang (28.2 g ± 2.3), and KUB (28.1 g ± 2.8). Furthermore, the live weight uniformity on the BS DOC averaged 71% across all groups, with the highest uniformity being BS-3 (87%). Murung Panggang had a higher live weight uniformity (82%) compared to Merawang A and B (42%, 64%) and KUB (63%). The average coefficient variance was less than 10%, except for Merawang A and BS-2 with 13.09% and 11.35%, respectively. In conclusion, the DOC of the crossbred (BS) was more uniform than its parental groups. However, to distinguish the significantly different in the chicken live weight in early stage, further analysis needs to be conducted in the future.","PeriodicalId":18160,"journal":{"name":"Malaysian applied biology","volume":"47 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-06-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"85544951","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-06-30DOI: 10.55230/mabjournal.v52i2.2619
Pornhathai Putthawan, Surachai Chaiso
This study was aimed at developing a protein-rich food formulation for the elderly using ingredients derived from soybean, sacha inchi, wheat flour, quinoa, and perilla seed. First, the protein content of all ingredients was analyzed. The results showed that the highest protein level (48.54%) was seen in sacha inchi. Then, sensory test by elderly adults was evaluated. The formulation which had the highest sensory acceptance comprised 33% soybean, 40% sacha inchi, 20% wheat flour, 5% quinoa, and 2% perilla seed. The effect of stabilizers (xanthan gum and sodium alginate) at levels of 0.1 and 0.2%, respectively, was studied. It was found that 0.1% sodium alginate produced the highest sensory score. Measurements of the texture and water absorption of the formulation showed that the values for hardness, cohesiveness, springiness, adhesiveness, chewiness and gumminess were 1003, 0.25, 0.45, 0.17, 110, and 222, respectively, while water absorption was 51.10%. An aqueous extract of Amaranthus dubius was subjected to analysis of levels of polyphenols and anthocyanins, as well as antioxidant capacity and cytotoxicity. The aqueous extract had polyphenol, anthocyanin, DPPH radical-scavenging and FRAP levels of 41.13 µg GAE/mL, 458 mg/L, 62.7%, and 14.8 µg Trolox/mL, respectively. At a concentration of 2000 µg/mL, the crude extract exerted 22% average anti-proliferative effect on P388, KB, Hela and HepG2 cells. Different extract levels were added to the product, and the acceptability of the concentrated extract was re-evaluated. The results showed that the concentrated extract at 0.5% level of incorporation had the highest acceptance rating as a meat analogue. The energy per 100 g of the plant meat sample was 247.95 kcal, while its contents of protein, fat, carbohydrate, ash, and dietary fibre were 24.71, 6.27, 23.17, 2.52 and 6.76%, respectively. Thus, the plant meat product supplemented with A. dubius extract could be an alternative and safe diet for the elderly.
{"title":"Development of A Plant-Based Meat Product Supplemented With Red Amaranth Antioxidants For The Elderly","authors":"Pornhathai Putthawan, Surachai Chaiso","doi":"10.55230/mabjournal.v52i2.2619","DOIUrl":"https://doi.org/10.55230/mabjournal.v52i2.2619","url":null,"abstract":"This study was aimed at developing a protein-rich food formulation for the elderly using ingredients derived from soybean, sacha inchi, wheat flour, quinoa, and perilla seed. First, the protein content of all ingredients was analyzed. The results showed that the highest protein level (48.54%) was seen in sacha inchi. Then, sensory test by elderly adults was evaluated. The formulation which had the highest sensory acceptance comprised 33% soybean, 40% sacha inchi, 20% wheat flour, 5% quinoa, and 2% perilla seed. The effect of stabilizers (xanthan gum and sodium alginate) at levels of 0.1 and 0.2%, respectively, was studied. It was found that 0.1% sodium alginate produced the highest sensory score. Measurements of the texture and water absorption of the formulation showed that the values for hardness, cohesiveness, springiness, adhesiveness, chewiness and gumminess were 1003, 0.25, 0.45, 0.17, 110, and 222, respectively, while water absorption was 51.10%. An aqueous extract of Amaranthus dubius was subjected to analysis of levels of polyphenols and anthocyanins, as well as antioxidant capacity and cytotoxicity. The aqueous extract had polyphenol, anthocyanin, DPPH radical-scavenging and FRAP levels of 41.13 µg GAE/mL, 458 mg/L, 62.7%, and 14.8 µg Trolox/mL, respectively. At a concentration of 2000 µg/mL, the crude extract exerted 22% average anti-proliferative effect on P388, KB, Hela and HepG2 cells. Different extract levels were added to the product, and the acceptability of the concentrated extract was re-evaluated. The results showed that the concentrated extract at 0.5% level of incorporation had the highest acceptance rating as a meat analogue. The energy per 100 g of the plant meat sample was 247.95 kcal, while its contents of protein, fat, carbohydrate, ash, and dietary fibre were 24.71, 6.27, 23.17, 2.52 and 6.76%, respectively. Thus, the plant meat product supplemented with A. dubius extract could be an alternative and safe diet for the elderly.","PeriodicalId":18160,"journal":{"name":"Malaysian applied biology","volume":"62 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-06-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"73575602","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-06-30DOI: 10.55230/mabjournal.v52i2.2542
Liana Mohd Zulkamal, Nurul Afifah Ainna Zolhalim, Farizan Aris, Nurul Aili Zakaria, F. Yusof, D. Ibrahim, M. T. M. Jalil
Clitoria ternatea, sometimes referred to as the Asian pigeon wings blue pea, the butterfly pea, or the Darwin pea, is a Fabaceae plant species that has been shown to possess antibacterial effects against several pathogenic microbes. Hence, the present study has been carried out to access the antibacterial activity of C. ternatea flower extracted with water and methanol against pathogenic bacteria. The well and disk diffusion assays were performed to determine the antibacterial activity of C. ternatea flower extracts. The efficacy of the extracts was then evaluated via broth microdilution assay to obtain MIC and MBC values and the growth reduction assay. Meanwhile, the DPPH scavenging test was used to assess the antioxidant activity of the crude extracts. The results of the well and disc diffusion assays showed that Gram-positive bacteria were more sensitive to both extracts compared to Gram-negative bacteria. Meanwhile, the methanolic extract showed higher antibacterial activity on both Gram-positive and Gram-negative bacteria compared to the aqueous extract. The results of the MIC and MBC tests showed that the methanolic extract was bactericidal to both Gram-positive and Gram-negative bacteria. The aqueous extract, however, demonstrated bacteriostatic activity against Gram-negative bacteria and bactericidal activity solely against Gram-positive bacteria. After a 24-h exposure period, a growth reduction assay showed that the methanolic extract could suppress both Gram-positive and Gram-negative bacteria by up to 99%. Meanwhile, the aqueous extract showed an inhibition percentage value ranging from 75% to 96% after an incubation period. The aqueous extract had the lowest antioxidant activity, with an EC50 value of 87.78 µg/mL, whereas the methanolic extract had a fair amount of antioxidant activity when compared to the control (quercetin), according to the DPPH scavenging assay. The present study suggests that C. ternatea extracts as a potential antibacterial agent against pathogenic bacteria with significant antioxidant activity and this activity may be due to the presence of anthocyanin and its derivatives.
{"title":"Bioactivity of Clitoria ternatea Crude Extracts Against Pathogenic Bacteria","authors":"Liana Mohd Zulkamal, Nurul Afifah Ainna Zolhalim, Farizan Aris, Nurul Aili Zakaria, F. Yusof, D. Ibrahim, M. T. M. Jalil","doi":"10.55230/mabjournal.v52i2.2542","DOIUrl":"https://doi.org/10.55230/mabjournal.v52i2.2542","url":null,"abstract":"Clitoria ternatea, sometimes referred to as the Asian pigeon wings blue pea, the butterfly pea, or the Darwin pea, is a Fabaceae plant species that has been shown to possess antibacterial effects against several pathogenic microbes. Hence, the present study has been carried out to access the antibacterial activity of C. ternatea flower extracted with water and methanol against pathogenic bacteria. The well and disk diffusion assays were performed to determine the antibacterial activity of C. ternatea flower extracts. The efficacy of the extracts was then evaluated via broth microdilution assay to obtain MIC and MBC values and the growth reduction assay. Meanwhile, the DPPH scavenging test was used to assess the antioxidant activity of the crude extracts. The results of the well and disc diffusion assays showed that Gram-positive bacteria were more sensitive to both extracts compared to Gram-negative bacteria. Meanwhile, the methanolic extract showed higher antibacterial activity on both Gram-positive and Gram-negative bacteria compared to the aqueous extract. The results of the MIC and MBC tests showed that the methanolic extract was bactericidal to both Gram-positive and Gram-negative bacteria. The aqueous extract, however, demonstrated bacteriostatic activity against Gram-negative bacteria and bactericidal activity solely against Gram-positive bacteria. After a 24-h exposure period, a growth reduction assay showed that the methanolic extract could suppress both Gram-positive and Gram-negative bacteria by up to 99%. Meanwhile, the aqueous extract showed an inhibition percentage value ranging from 75% to 96% after an incubation period. The aqueous extract had the lowest antioxidant activity, with an EC50 value of 87.78 µg/mL, whereas the methanolic extract had a fair amount of antioxidant activity when compared to the control (quercetin), according to the DPPH scavenging assay. The present study suggests that C. ternatea extracts as a potential antibacterial agent against pathogenic bacteria with significant antioxidant activity and this activity may be due to the presence of anthocyanin and its derivatives.","PeriodicalId":18160,"journal":{"name":"Malaysian applied biology","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-06-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"90106023","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-06-30DOI: 10.55230/mabjournal.v52i2.1994
R. Soelistijono, Angga Wulindra Jakti, Agus Budiyono, E. Suprapti, Daryanti
Orchids are cultivated by tissue culture because their seeds do not have food reserves (endosperm) for their growth. All nutrient requirements are obtained from tissue culture media. Propagation by tissue culture causes them to have no association with Rhizoctonia mycorrhizae, which are needed in their growth so that they can interfere with orchid growth both vegetatively and generatively. Rhizoctonia mycorrhizae are a group of Rhizoctonia sp. which form a mutual symbiotic association with orchids. Each orchid has a different association with Rhizoctonia mycorrhizae. At the time of association with orchids, Rhizoctonia mycorrhizae will form a peloton structure in the orchid root cortex, which supplies several nutrients needed by orchids from their environment during drought. Rhizoctonia mycorrhizae inoculation has been carried out on orchid seedlings of the same species orchids in vitro. However, Rhizoctonia mycorrhizae inoculation has never been carried out on seedlings of different species of orchid using a simpler spraying method. The success of simpler Rhizoctonia mycorrhizae inoculation on Dendrobium aggregatum seedlings might provide an understanding of the increased resistance of orchids to water stress. The materials used were isolates of Rhizoctonia mycorrhizae isolated from the roots of Dendrobium lasiantera and seedlings of Dendrobium aggregatum aged 6 months. The seedlings consisted of two groups. The former included seedlings exposed to Rhizoctonia mycorrhizae (M1) and watering with the intervals of 1, 2, and 3 days and those treated without Rhizoctonia mycorrhizae (M0) but with watering at the same intervals. The experiment used RCBD with 14 replications. Meanwhile, the control group was not exposed to both Rhizoctonia mycorrhizae and watering. The results showed that Rhizoctonia mycorrhizae inoculation had a significant effect on seedling height, leaf length, number of roots, and fresh weight of seedlings. Meanwhile, the watering interval did not give a real effect.
{"title":"The Uses of Rhizoctonia Mycorrhizae As a Dry Resistance Induction of Dendrobium aggregatum Seedlings","authors":"R. Soelistijono, Angga Wulindra Jakti, Agus Budiyono, E. Suprapti, Daryanti","doi":"10.55230/mabjournal.v52i2.1994","DOIUrl":"https://doi.org/10.55230/mabjournal.v52i2.1994","url":null,"abstract":"Orchids are cultivated by tissue culture because their seeds do not have food reserves (endosperm) for their growth. All nutrient requirements are obtained from tissue culture media. Propagation by tissue culture causes them to have no association with Rhizoctonia mycorrhizae, which are needed in their growth so that they can interfere with orchid growth both vegetatively and generatively. Rhizoctonia mycorrhizae are a group of Rhizoctonia sp. which form a mutual symbiotic association with orchids. Each orchid has a different association with Rhizoctonia mycorrhizae. At the time of association with orchids, Rhizoctonia mycorrhizae will form a peloton structure in the orchid root cortex, which supplies several nutrients needed by orchids from their environment during drought. Rhizoctonia mycorrhizae inoculation has been carried out on orchid seedlings of the same species orchids in vitro. However, Rhizoctonia mycorrhizae inoculation has never been carried out on seedlings of different species of orchid using a simpler spraying method. The success of simpler Rhizoctonia mycorrhizae inoculation on Dendrobium aggregatum seedlings might provide an understanding of the increased resistance of orchids to water stress. The materials used were isolates of Rhizoctonia mycorrhizae isolated from the roots of Dendrobium lasiantera and seedlings of Dendrobium aggregatum aged 6 months. The seedlings consisted of two groups. The former included seedlings exposed to Rhizoctonia mycorrhizae (M1) and watering with the intervals of 1, 2, and 3 days and those treated without Rhizoctonia mycorrhizae (M0) but with watering at the same intervals. The experiment used RCBD with 14 replications. Meanwhile, the control group was not exposed to both Rhizoctonia mycorrhizae and watering. The results showed that Rhizoctonia mycorrhizae inoculation had a significant effect on seedling height, leaf length, number of roots, and fresh weight of seedlings. Meanwhile, the watering interval did not give a real effect.","PeriodicalId":18160,"journal":{"name":"Malaysian applied biology","volume":"24 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-06-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"76688642","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-06-30DOI: 10.55230/mabjournal.v52i2.2646
Nurul Alina Muhamad Suhaini, Mohd Feiz Pauzi, Siti Norazlina Juhari, K. S. Mohd, Noor Azlina Abu Bakar
Geniotrigona thoracica is a stingless bee species of Trigona genus. Propolis resinous is a natural product obtained from a honeybees hive with geographical and floral specifications or exudate as such by-products resulting from a variety of botanical processes. Despite its long use for a variety of health conditions, the toxicity profile of propolis sourced from Malaysian stingless bees has not been sufficiently evaluated. For in vivo toxicity assessment, the acute oral toxicity on the effects of aqueous propolis extracts (APE) was examined. Male mice swiss strain, were subjected to acute toxicity testing for 14 days. The APE at doses of 400, 1000 and 2000 mg/kg body weight was supplemented daily to the mice through oral gavage. The clinical signs of toxicity and general behaviour, body weight, relative organ weight, and histopathology changes were investigated. In vivo study was focused on the acute toxicity testing group consisting of 4 groups including Normal (NS), 400 mg/kg (APE 400), 1000 mg/kg (APE 1000) dan 2000 mg/kg (APE 2000). Regarding the toxicity profile, it is proposed that APE supplementation did not induce any mortality and no visible signs of toxicity. No significant changes in the body and relative organ weight were recorded. All the internal organs of the mice were macroscopically healthy with no gross lesion. Likewise, histopathological examinations of the kidney showed mild to moderate histological lesions. Interestingly, the lesion was adverse with an increased dosage of the extract supplementation. This study proposed APE has considerable anti-inflammatory activities. It also demonstrated that the propolis extract is relatively safe to be consumed orally at a dose of 2000 mg/kg body weight.
{"title":"In Vivo Toxicity Study on The Effects of Aqueous Propolis Extract From Malaysian Stingless Bee (Geniotrigona thoracica) in Mice","authors":"Nurul Alina Muhamad Suhaini, Mohd Feiz Pauzi, Siti Norazlina Juhari, K. S. Mohd, Noor Azlina Abu Bakar","doi":"10.55230/mabjournal.v52i2.2646","DOIUrl":"https://doi.org/10.55230/mabjournal.v52i2.2646","url":null,"abstract":"Geniotrigona thoracica is a stingless bee species of Trigona genus. Propolis resinous is a natural product obtained from a honeybees hive with geographical and floral specifications or exudate as such by-products resulting from a variety of botanical processes. Despite its long use for a variety of health conditions, the toxicity profile of propolis sourced from Malaysian stingless bees has not been sufficiently evaluated. For in vivo toxicity assessment, the acute oral toxicity on the effects of aqueous propolis extracts (APE) was examined. Male mice swiss strain, were subjected to acute toxicity testing for 14 days. The APE at doses of 400, 1000 and 2000 mg/kg body weight was supplemented daily to the mice through oral gavage. The clinical signs of toxicity and general behaviour, body weight, relative organ weight, and histopathology changes were investigated. In vivo study was focused on the acute toxicity testing group consisting of 4 groups including Normal (NS), 400 mg/kg (APE 400), 1000 mg/kg (APE 1000) dan 2000 mg/kg (APE 2000). Regarding the toxicity profile, it is proposed that APE supplementation did not induce any mortality and no visible signs of toxicity. No significant changes in the body and relative organ weight were recorded. All the internal organs of the mice were macroscopically healthy with no gross lesion. Likewise, histopathological examinations of the kidney showed mild to moderate histological lesions. Interestingly, the lesion was adverse with an increased dosage of the extract supplementation. This study proposed APE has considerable anti-inflammatory activities. It also demonstrated that the propolis extract is relatively safe to be consumed orally at a dose of 2000 mg/kg body weight.","PeriodicalId":18160,"journal":{"name":"Malaysian applied biology","volume":"89 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-06-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"85536202","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-06-30DOI: 10.55230/mabjournal.v52i2.2520
Khairunnisa Mohammad Hamdi, S. Lihan, Stanley Sait, Scholastica Ramih, Nur Azzah Osman, N. N. Mohamad, Tay Meng Guan, Fazia Mohamad Sinang, Hashimatul Fatma Hashim
Water quality monitoring of a particular river is crucially important to determine if the river water meets the requirement for its designated uses. Therefore, an assessment of the physiochemical water quality at Satow Waterfall, Sarawak, and the prevalence of antibiotic-resistant bacteria (ARB) was conducted to corroborate the safety of the water from the waterfall. A total of 42 water samples were collected at the subsurface water of the upstream, midstream, and downstream on two sampling trips in June 2021 (dry season) and December 2021 (wet season), with six months intervals. The water quality index (WQI) of the sampling stations ranged from 92.60 to 95, classifying the water under Class I. However, the water is microbiologically polluted, with the highest coliform count of 2.59 × 105 CFU/mL recorded at the middle stream during the wet season, which has surpassed the regulatory standard set by the Malaysian Department of Environment (DOE). A total of 54 bacterial isolates were chosen as representatives and identified by 16S rRNA sequencing, which confirmed the existence of 21 bacterial genera. All identified isolates were tested against 15 antibiotics of various classes employing the Clinical and Laboratory Standards Institute (CLSI) protocols. The antibiotic susceptibility test (AST) demonstrated substantial resistance to sulfamethoxazole (48%) and erythromycin (48%), as well as high susceptibility to chloramphenicol (84%), levofloxacin (60%) and ofloxacin (60%). The Multiple Antibiotic Resistance Index (MARI) evaluations demonstrated the MARI varied from 0-0.60, with 33% of the isolates having a MARI greater than 0.2. In this study, the occurrence of ARB in a water environment where there is an undisclosed source of antibiotic utilization is still inevitable, highlighting the necessity to constantly monitor the water quality and the pervasiveness of ARB in recreational water.
{"title":"Water Quality Analysis and The Occurrence of Antibiotic-Resistant Bacteria (ARB) From Satow Waterfall in Bau, Malaysian Borneo","authors":"Khairunnisa Mohammad Hamdi, S. Lihan, Stanley Sait, Scholastica Ramih, Nur Azzah Osman, N. N. Mohamad, Tay Meng Guan, Fazia Mohamad Sinang, Hashimatul Fatma Hashim","doi":"10.55230/mabjournal.v52i2.2520","DOIUrl":"https://doi.org/10.55230/mabjournal.v52i2.2520","url":null,"abstract":"Water quality monitoring of a particular river is crucially important to determine if the river water meets the requirement for its designated uses. Therefore, an assessment of the physiochemical water quality at Satow Waterfall, Sarawak, and the prevalence of antibiotic-resistant bacteria (ARB) was conducted to corroborate the safety of the water from the waterfall. A total of 42 water samples were collected at the subsurface water of the upstream, midstream, and downstream on two sampling trips in June 2021 (dry season) and December 2021 (wet season), with six months intervals. The water quality index (WQI) of the sampling stations ranged from 92.60 to 95, classifying the water under Class I. However, the water is microbiologically polluted, with the highest coliform count of 2.59 × 105 CFU/mL recorded at the middle stream during the wet season, which has surpassed the regulatory standard set by the Malaysian Department of Environment (DOE). A total of 54 bacterial isolates were chosen as representatives and identified by 16S rRNA sequencing, which confirmed the existence of 21 bacterial genera. All identified isolates were tested against 15 antibiotics of various classes employing the Clinical and Laboratory Standards Institute (CLSI) protocols. The antibiotic susceptibility test (AST) demonstrated substantial resistance to sulfamethoxazole (48%) and erythromycin (48%), as well as high susceptibility to chloramphenicol (84%), levofloxacin (60%) and ofloxacin (60%). The Multiple Antibiotic Resistance Index (MARI) evaluations demonstrated the MARI varied from 0-0.60, with 33% of the isolates having a MARI greater than 0.2. In this study, the occurrence of ARB in a water environment where there is an undisclosed source of antibiotic utilization is still inevitable, highlighting the necessity to constantly monitor the water quality and the pervasiveness of ARB in recreational water.","PeriodicalId":18160,"journal":{"name":"Malaysian applied biology","volume":"35 1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-06-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"73354564","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-06-30DOI: 10.55230/mabjournal.v52i2.2614
Nur Anisah Johari, Mohd Shafiq Aazmi, M. Yahya
The present work was performed to determine the impacts of commercial disinfectants against biomass, viability, and biochemical composition of Salmonella enterica serovar Thyphimurium ATCC14028 biofilm. Salmonella Thyphimurium biofilm grown in microplates was exposed to commercial disinfectants namely sodium hypochlorite, benzalkonium chloride, chloroxylenol, and sodium dodecyl-benzene sulfonate-based disinfectants. Biofilm biomass, biofilm viability, and biochemical composition of the biofilm were determined using crystal violet assay, resazurin assay and Fourier transform infrared (FTIR) spectroscopy respectively. Results demonstrated that, among four commercial disinfectants, chloroxylenol-based disinfectant showed the highest inhibition against S. Thyphimurium biofilm. It remarkably hindered biofilm biomass and biofilm viability at all tested concentrations (0.78%-25%). Half-maximal biofilm inhibitory concentration (BIC50) of chloroxylenol-based disinfectant (5.06%) was found to be the lowest among the tested disinfectants. Meanwhile, S. Thyphimurium biofilm treated with chloroxylenol-based disinfectant exhibited changes in FTIR spectral peaks associated with lipid (1460 cm-1), protein (630 cm-1, 702 cm-1, 1550 cm-1 & 1650 cm-1), and nucleic acid (1080 cm-1 & 1229 cm-1). The findings of the present study suggest that the inhibition of chloroxylenol-based disinfectant against S. Thyphimurium biofilm is mediated by structural changes of biofilm.
{"title":"FTIR Spectroscopic Study Of Inhibition of Chloroxylenol-Based Disinfectant Against Salmonella enterica serovar Thyphimurium Biofilm","authors":"Nur Anisah Johari, Mohd Shafiq Aazmi, M. Yahya","doi":"10.55230/mabjournal.v52i2.2614","DOIUrl":"https://doi.org/10.55230/mabjournal.v52i2.2614","url":null,"abstract":"The present work was performed to determine the impacts of commercial disinfectants against biomass, viability, and biochemical composition of Salmonella enterica serovar Thyphimurium ATCC14028 biofilm. Salmonella Thyphimurium biofilm grown in microplates was exposed to commercial disinfectants namely sodium hypochlorite, benzalkonium chloride, chloroxylenol, and sodium dodecyl-benzene sulfonate-based disinfectants. Biofilm biomass, biofilm viability, and biochemical composition of the biofilm were determined using crystal violet assay, resazurin assay and Fourier transform infrared (FTIR) spectroscopy respectively. Results demonstrated that, among four commercial disinfectants, chloroxylenol-based disinfectant showed the highest inhibition against S. Thyphimurium biofilm. It remarkably hindered biofilm biomass and biofilm viability at all tested concentrations (0.78%-25%). Half-maximal biofilm inhibitory concentration (BIC50) of chloroxylenol-based disinfectant (5.06%) was found to be the lowest among the tested disinfectants. Meanwhile, S. Thyphimurium biofilm treated with chloroxylenol-based disinfectant exhibited changes in FTIR spectral peaks associated with lipid (1460 cm-1), protein (630 cm-1, 702 cm-1, 1550 cm-1 & 1650 cm-1), and nucleic acid (1080 cm-1 & 1229 cm-1). The findings of the present study suggest that the inhibition of chloroxylenol-based disinfectant against S. Thyphimurium biofilm is mediated by structural changes of biofilm.","PeriodicalId":18160,"journal":{"name":"Malaysian applied biology","volume":"10 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-06-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"88920394","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-06-30DOI: 10.55230/mabjournal.v52i2.2565
C. Chong, Muhamad Faris Ab Aziz, M. R. Ismail‐Fitry
Sardine fish patty is a processed seafood product that easily spoiled because of oxidation. Oregano, cloves, cinnamon, turmeric, and green tea contain antioxidant properties that could preserve the fish patty. This study aimed to incorporate these ingredients into sardine fish patties and investigate their effects on the physicochemical properties, sensory evaluation, and lipid oxidation of the patties. The fresh sardine patties were treated with 0.5% of oregano (PWO), cloves (PWC), cinnamon (PWCI), turmeric (PWT) or green tea (PWGT) before being cooked, cooled and chill-stored for 12 days. No significant effects of the treatments were observed on moisture content, cooking yield, and shrinkage of the patties. The incorporation of turmeric significantly (P<0.05) affected all colour parameters on the patties (PWT). Although PWGT showed slightly increased values in some texture profiling parameters, the PWGT also showed positive acceptability regarding sensory evaluation for most of the eating quality parameters. A significant (P<0.05) reduction in TBARS values in the patties incorporated with green tea (PWGT), while increased scavenging activity values of the PWGT and cloves (PWC) were observed. Thus, the study implied that green tea demonstrated a good effect on sardine fish patty, as compared to spices, which could be a potential natural preservative to preserve the fish product during storage.
{"title":"Physicochemical, Sensorial and Antioxidant Properties of Sardine Fish Patties Incorporated with Different Natural Additives","authors":"C. Chong, Muhamad Faris Ab Aziz, M. R. Ismail‐Fitry","doi":"10.55230/mabjournal.v52i2.2565","DOIUrl":"https://doi.org/10.55230/mabjournal.v52i2.2565","url":null,"abstract":"Sardine fish patty is a processed seafood product that easily spoiled because of oxidation. Oregano, cloves, cinnamon, turmeric, and green tea contain antioxidant properties that could preserve the fish patty. This study aimed to incorporate these ingredients into sardine fish patties and investigate their effects on the physicochemical properties, sensory evaluation, and lipid oxidation of the patties. The fresh sardine patties were treated with 0.5% of oregano (PWO), cloves (PWC), cinnamon (PWCI), turmeric (PWT) or green tea (PWGT) before being cooked, cooled and chill-stored for 12 days. No significant effects of the treatments were observed on moisture content, cooking yield, and shrinkage of the patties. The incorporation of turmeric significantly (P<0.05) affected all colour parameters on the patties (PWT). Although PWGT showed slightly increased values in some texture profiling parameters, the PWGT also showed positive acceptability regarding sensory evaluation for most of the eating quality parameters. A significant (P<0.05) reduction in TBARS values in the patties incorporated with green tea (PWGT), while increased scavenging activity values of the PWGT and cloves (PWC) were observed. Thus, the study implied that green tea demonstrated a good effect on sardine fish patty, as compared to spices, which could be a potential natural preservative to preserve the fish product during storage.","PeriodicalId":18160,"journal":{"name":"Malaysian applied biology","volume":"33 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-06-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"73603226","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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