A pot experiment was conducted to see the effect of sodicity and iron levels on cowpea. Four levels of sodium adsorption ratio (SAR) 10, 15, 20, 25 and iron (Fe) control, 5, 10, 15 and 20 kg Fe ha-1) were tested in completely randomized design with three replications. The results indicated that application of soil sodicity having SAR 15 recorded the maximum yield, phosphorus and potassium content and uptake and nitrogen uptake of cowpea over rest of the treatments. Application of Fe 20 kg ha-1 recorded the maximum and significantly higher yield, nitrogen, phosphorus and potassium content and uptake of cowpea over rest of the treatments. Among the treatment combinations combined application of SAR 15 with Fe @ +20 FeSo4.7H2O) proved superior in all these parameters over other treatments.
通过盆栽试验，研究了不同碱度和铁含量对豇豆生长的影响。采用完全随机设计，3个重复，试验4个水平的钠吸附比(SAR)分别为10、15、20、25和铁(Fe)对照(5、10、15和20 kg Fe ha-1)。结果表明，施用sa15土壤碱度的豇豆产量、磷、钾含量、吸收量和氮素吸收量均高于其他处理。施用Fe 20 kg hm -1处理豇豆产量、氮、磷、钾含量和吸收量最高且显著高于其他处理。在处理组合中，SAR 15与Fe @ +20 (FeSo4.7H2O)的联合应用在所有这些参数上都优于其他处理。
{"title":"Impact of sodium and iron levels on yield, nutrient content and uptake by cowpea (Vigna unguiculata L.)","authors":"Kiran Doodhwal, B. Yadav, M. Yadav, M. Choudhary","doi":"10.18805/LR-4004","DOIUrl":"https://doi.org/10.18805/LR-4004","url":null,"abstract":"A pot experiment was conducted to see the effect of sodicity and iron levels on cowpea. Four levels of sodium adsorption ratio (SAR) 10, 15, 20, 25 and iron (Fe) control, 5, 10, 15 and 20 kg Fe ha-1) were tested in completely randomized design with three replications. The results indicated that application of soil sodicity having SAR 15 recorded the maximum yield, phosphorus and potassium content and uptake and nitrogen uptake of cowpea over rest of the treatments. Application of Fe 20 kg ha-1 recorded the maximum and significantly higher yield, nitrogen, phosphorus and potassium content and uptake of cowpea over rest of the treatments. Among the treatment combinations combined application of SAR 15 with Fe @ +20 FeSo4.7H2O) proved superior in all these parameters over other treatments.","PeriodicalId":18181,"journal":{"name":"Legume Research","volume":null,"pages":null},"PeriodicalIF":0.8,"publicationDate":"2018-07-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"72851780","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
D. Mandal, P. Bhattacharyya, R. Das, S. Bhattacharyya
Twelve Simple Sequence Repeat (SSR) markers along with twelve morphological markers were used to assess the diversity among thirteen screened genotypes both moderate resistant and susceptible against stemphylium blight of indigenous microsperma germplasm (i.e. pilosae) type of Lentil (Lens culinaris Medik.) at BCKV, West Bengal. Polymorphic Information Content (PIC) of SSR primers ranged from 0.77 (SSR 317-2) to 0.15 (PBA LC 0233). The maximum Jaccard’s similarity was recorded between L-4769 and Ranjan (approximately 100%) whereas minimum was between K-75 and VL 126 (0.11). Genotypes K-75 also showed less similarity with DL 14-2 and VL 151 (0.17). Another popular variety WBL 77 showed minimum similarity with moderately resistant genotypes VL 151 and DL 14-2 (0.17). While using morphological characters as the basis of diversity analysis, Euclidean coefficient revealed that high yielding cultivar Subrata was maximum diverse with genotype VL 151(7.12). Asha also showed diversity with VL 151(6.31) whereas VL 151 had dissimilarity coefficient of 6.21 with K-75. Precoz and VL 126 has dissimilarity coefficient of 6.19. On the basis of above findings it could be said that diverse parental pairs VL 151 and K 75 and VL 151 and Subrata / Asha could be used in hybridization programme or biotic stress breeding programme for developing Stemphylium resistant lentil cultivars in future.
{"title":"Diversity analysis by SSR markers and morphological markers among Stemphylium blight tolerance genotypes with high yielding cultivars of Lentil (Lens Culinaris Medik)","authors":"D. Mandal, P. Bhattacharyya, R. Das, S. Bhattacharyya","doi":"10.18805/LR-4029","DOIUrl":"https://doi.org/10.18805/LR-4029","url":null,"abstract":"Twelve Simple Sequence Repeat (SSR) markers along with twelve morphological markers were used to assess the diversity among thirteen screened genotypes both moderate resistant and susceptible against stemphylium blight of indigenous microsperma germplasm (i.e. pilosae) type of Lentil (Lens culinaris Medik.) at BCKV, West Bengal. Polymorphic Information Content (PIC) of SSR primers ranged from 0.77 (SSR 317-2) to 0.15 (PBA LC 0233). The maximum Jaccard’s similarity was recorded between L-4769 and Ranjan (approximately 100%) whereas minimum was between K-75 and VL 126 (0.11). Genotypes K-75 also showed less similarity with DL 14-2 and VL 151 (0.17). Another popular variety WBL 77 showed minimum similarity with moderately resistant genotypes VL 151 and DL 14-2 (0.17). While using morphological characters as the basis of diversity analysis, Euclidean coefficient revealed that high yielding cultivar Subrata was maximum diverse with genotype VL 151(7.12). Asha also showed diversity with VL 151(6.31) whereas VL 151 had dissimilarity coefficient of 6.21 with K-75. Precoz and VL 126 has dissimilarity coefficient of 6.19. On the basis of above findings it could be said that diverse parental pairs VL 151 and K 75 and VL 151 and Subrata / Asha could be used in hybridization programme or biotic stress breeding programme for developing Stemphylium resistant lentil cultivars in future.","PeriodicalId":18181,"journal":{"name":"Legume Research","volume":null,"pages":null},"PeriodicalIF":0.8,"publicationDate":"2018-07-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"83275402","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Fenugreek (Trigonella foenum-graecum) is one of the most important and primitive medicinal plants belonging to family fabaceae and is recognized as one of the most prominent seed spices. Development, identification and classification of entirely new set of SSRs are a very expensive and time consuming process for a crop like fenugreek. Instead, a novel set of SSRs can be achieved with ease in less expense by transferring the microsatellite loci of the related species in fenugreek. SSR’s of two major crops, carrot and celery belonging to apiaceae family are available in public domain for cross-species and inter-family investigation. In the present study, we examined transferability of 100 carrot SSR loci in fenugreek of which 19% of SSR’s amplified, where 5 primers were monomorphic. 14 SSRs were polymorphic in nature and the bands ranged between 2 to 5. GSSR-87, GSSR-92, GSSR-136 and BSSR-8 showed monomorphic bands and were specific for fenugreek genotypes and can be regarded as a dominant tool for fenugreek breeding and genetic studies. However, GSSR-81 and GSSR-96 were most polymorphic in nature and can be utilized further to distinguish fenugreek from other seed spices. The analysis, revealed optimum level of cross-species transferability (>35.2%) in fenugreek, where no SSR resource is available till date, initiating cross transferability testing from the enriched SSR database of carrot.
{"title":"Identification and characterization of microsatellite markers in fenugreek: An inter-family amplification","authors":"G. Jethra, S. Choudhary, V. Sharma","doi":"10.18805/LR-4024","DOIUrl":"https://doi.org/10.18805/LR-4024","url":null,"abstract":"Fenugreek (Trigonella foenum-graecum) is one of the most important and primitive medicinal plants belonging to family fabaceae and is recognized as one of the most prominent seed spices. Development, identification and classification of entirely new set of SSRs are a very expensive and time consuming process for a crop like fenugreek. Instead, a novel set of SSRs can be achieved with ease in less expense by transferring the microsatellite loci of the related species in fenugreek. SSR’s of two major crops, carrot and celery belonging to apiaceae family are available in public domain for cross-species and inter-family investigation. In the present study, we examined transferability of 100 carrot SSR loci in fenugreek of which 19% of SSR’s amplified, where 5 primers were monomorphic. 14 SSRs were polymorphic in nature and the bands ranged between 2 to 5. GSSR-87, GSSR-92, GSSR-136 and BSSR-8 showed monomorphic bands and were specific for fenugreek genotypes and can be regarded as a dominant tool for fenugreek breeding and genetic studies. However, GSSR-81 and GSSR-96 were most polymorphic in nature and can be utilized further to distinguish fenugreek from other seed spices. The analysis, revealed optimum level of cross-species transferability (>35.2%) in fenugreek, where no SSR resource is available till date, initiating cross transferability testing from the enriched SSR database of carrot.","PeriodicalId":18181,"journal":{"name":"Legume Research","volume":null,"pages":null},"PeriodicalIF":0.8,"publicationDate":"2018-07-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"80928403","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
An experiment conducted during 2011-12 and 2012-13 under the ongoing All India Coordinated Research Project on Long-Term Fertilizer Experiment with soybean-safflower cropping sequence in Vertisol (Typic haplusterts) that commenced form 2006-07 at Vasantrao Naik Marathwada Krishi Vidyapeeth, Parbhani (M.S.) showed that the nutrients availability in soil, nutrient uptake, yield attributes and yield of soybean were significantly influenced by FYM along with 100% NPK application. The results revealed that the pods per plant (48.65), plant height (49.77 cm plant-1), grain (26.97 q ha-1) and straw (24.65 q ha-1) yield of soybean were significantly increased with 100% NPK+FYM@ 5 Mg ha-1. Conjoint use of FYM with 100% NPK improved the soil properties with respect to bulk density, organic carbon, exchangeable (Ca, Mg, Na & K), cation exchange capacity (CEC), base saturation (BS), available N, P, K, S, Zn and Fe over initial value of soil. However, a significant buildup of available Zn due to the application of zinc sulphate along with 100% NPK as compared to all other treatments. N, P, K, S, Zn and Fe uptake of soybean were increased significantly with the application of 100 % NPK+FYM@ 5 Mg ha 1 than other treatment. It can be concluded from these experiment that the balanced use of fertilizers continuously either alone or in combination with organic manure is necessary for sustaining soil fertility and productivity of crop.
{"title":"Impact of Long-term fertilizer application on soil fertility, nutrient uptake, growth and productivity of soybean under soybean-safflower cropping sequence in Vertisol","authors":"N. Meshram, S. Ismail, S. Shirale, V. D. Patil","doi":"10.18805/LR-3676","DOIUrl":"https://doi.org/10.18805/LR-3676","url":null,"abstract":"An experiment conducted during 2011-12 and 2012-13 under the ongoing All India Coordinated Research Project on Long-Term Fertilizer Experiment with soybean-safflower cropping sequence in Vertisol (Typic haplusterts) that commenced form 2006-07 at Vasantrao Naik Marathwada Krishi Vidyapeeth, Parbhani (M.S.) showed that the nutrients availability in soil, nutrient uptake, yield attributes and yield of soybean were significantly influenced by FYM along with 100% NPK application. The results revealed that the pods per plant (48.65), plant height (49.77 cm plant-1), grain (26.97 q ha-1) and straw (24.65 q ha-1) yield of soybean were significantly increased with 100% NPK+FYM@ 5 Mg ha-1. Conjoint use of FYM with 100% NPK improved the soil properties with respect to bulk density, organic carbon, exchangeable (Ca, Mg, Na & K), cation exchange capacity (CEC), base saturation (BS), available N, P, K, S, Zn and Fe over initial value of soil. However, a significant buildup of available Zn due to the application of zinc sulphate along with 100% NPK as compared to all other treatments. N, P, K, S, Zn and Fe uptake of soybean were increased significantly with the application of 100 % NPK+FYM@ 5 Mg ha 1 than other treatment. It can be concluded from these experiment that the balanced use of fertilizers continuously either alone or in combination with organic manure is necessary for sustaining soil fertility and productivity of crop.","PeriodicalId":18181,"journal":{"name":"Legume Research","volume":null,"pages":null},"PeriodicalIF":0.8,"publicationDate":"2018-07-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"83250301","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
D. Mal, S. Datta, Pranali Tulsidas Bhaisare, R. Chatterjee, A. Chowdhury, J. C. Jana
The present experimentation was undertaken during pre-kharif season of 2013 and 2014 to study the varietal performance of cowpea in rice-wheat cropping system at Uttar Banga Krishi Viswavidyalaya, Pundibai, Cooch Behar, West Bengal after harvesting of wheat under zero tillage conditions and before rice sowing. Experiment was laid out in randomized block design with three replications by using eighteen varieties of cowpea. The result of pooled data revealed that all the growth, yield parameters and quality parameters varied significantly with respect to different varieties of cowpea. The longest and lowest vine length was noticed in Lafa Sundari Bangla (112.10 cm) and VU-5 (32.64 cm), minimum and maximum days for flowering, first harvesting and last harvesting was recorded in Kaberee and Pusa Phalguni Gold. Variety Girija and Deshi Lafa produced longest pod (45.40 cm), highest individual pod weight (14.29g). For number of pods per plant Triguna (31.07) was found highest which was statistically at par with Kanak (30.70), Kashi Kanchan (29.33). Maximum yield of 18.51 t/ha was obtained from Kashi Kanchan followed by Triguna (17.07 tonnes/ ha) and Kanak (16.69 tonnes/ha). Lafa Sundari Bangala recorded with highest beta carotene content (1037 IU/100g fresh pod), ascorbic acid content (20.17 mg/100g fresh pod) as well as highest protein content of 4.69 per cent. Hence, it is concluded that the cowpea variety Kashi Kanchan, Triguna and Kanak may be recommended for commercial cultivation in rice-wheat cropping system under terai zone of West Bengal as they produced higher yield (34.52 %, 24.06 % and 21.29 %, respectively) over the check variety Lafa Sundari Bangla.
{"title":"Performance of cowpea varieties under zero tillage conditions in rice-wheat cropping system","authors":"D. Mal, S. Datta, Pranali Tulsidas Bhaisare, R. Chatterjee, A. Chowdhury, J. C. Jana","doi":"10.18805/LR-3972","DOIUrl":"https://doi.org/10.18805/LR-3972","url":null,"abstract":"The present experimentation was undertaken during pre-kharif season of 2013 and 2014 to study the varietal performance of cowpea in rice-wheat cropping system at Uttar Banga Krishi Viswavidyalaya, Pundibai, Cooch Behar, West Bengal after harvesting of wheat under zero tillage conditions and before rice sowing. Experiment was laid out in randomized block design with three replications by using eighteen varieties of cowpea. The result of pooled data revealed that all the growth, yield parameters and quality parameters varied significantly with respect to different varieties of cowpea. The longest and lowest vine length was noticed in Lafa Sundari Bangla (112.10 cm) and VU-5 (32.64 cm), minimum and maximum days for flowering, first harvesting and last harvesting was recorded in Kaberee and Pusa Phalguni Gold. Variety Girija and Deshi Lafa produced longest pod (45.40 cm), highest individual pod weight (14.29g). For number of pods per plant Triguna (31.07) was found highest which was statistically at par with Kanak (30.70), Kashi Kanchan (29.33). Maximum yield of 18.51 t/ha was obtained from Kashi Kanchan followed by Triguna (17.07 tonnes/ ha) and Kanak (16.69 tonnes/ha). Lafa Sundari Bangala recorded with highest beta carotene content (1037 IU/100g fresh pod), ascorbic acid content (20.17 mg/100g fresh pod) as well as highest protein content of 4.69 per cent. Hence, it is concluded that the cowpea variety Kashi Kanchan, Triguna and Kanak may be recommended for commercial cultivation in rice-wheat cropping system under terai zone of West Bengal as they produced higher yield (34.52 %, 24.06 % and 21.29 %, respectively) over the check variety Lafa Sundari Bangla.","PeriodicalId":18181,"journal":{"name":"Legume Research","volume":null,"pages":null},"PeriodicalIF":0.8,"publicationDate":"2018-05-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"79199949","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
A field experiment was conducted for three years to study the effect of sowing dates, mulching and seed rates on nutrient uptake and productivity of soybean under irrigated conditions in sub-humid Punjab on a silty clay loam soil which was low in available N, medium in available P and available K. The experiment was conducted in a split plot design with the combination of three sowing dates and mulch in main plots and three seed rates in sub-plots with three replications on fixed site. The results revealed that sowing on June 30 and mulching recorded the highest number of pods plant-1.There was no significant influence of seed rates on yield attributes. Seed yield also remained at par under different sowing dates, mulching and seed rate treatments. Stover yield however, was significantly higher under the earlier sowing dates and mulching. Highest total N uptake was observed under June 30 sowing date whereas total P and K uptake was highest in May 20 sowing date. No significant effect of mulching was observed on total N and K uptake. Among seed rates maximum total N uptake was observed under 75 kg seed ha-1. The results suggest that soybean performed better when sown with 60 kg seed rate ha-1 on June 30 and mulched with wheat straw.
{"title":"Effect of sowing dates, mulching and seed rates on nutrient uptake and productivity of soybean in sub - humid Punjab, India","authors":"R. Sikka, J. Deol, Jagmohan Kaur, D. Singh","doi":"10.18805/LR-3731","DOIUrl":"https://doi.org/10.18805/LR-3731","url":null,"abstract":"A field experiment was conducted for three years to study the effect of sowing dates, mulching and seed rates on nutrient uptake and productivity of soybean under irrigated conditions in sub-humid Punjab on a silty clay loam soil which was low in available N, medium in available P and available K. The experiment was conducted in a split plot design with the combination of three sowing dates and mulch in main plots and three seed rates in sub-plots with three replications on fixed site. The results revealed that sowing on June 30 and mulching recorded the highest number of pods plant-1.There was no significant influence of seed rates on yield attributes. Seed yield also remained at par under different sowing dates, mulching and seed rate treatments. Stover yield however, was significantly higher under the earlier sowing dates and mulching. Highest total N uptake was observed under June 30 sowing date whereas total P and K uptake was highest in May 20 sowing date. No significant effect of mulching was observed on total N and K uptake. Among seed rates maximum total N uptake was observed under 75 kg seed ha-1. The results suggest that soybean performed better when sown with 60 kg seed rate ha-1 on June 30 and mulched with wheat straw.","PeriodicalId":18181,"journal":{"name":"Legume Research","volume":null,"pages":null},"PeriodicalIF":0.8,"publicationDate":"2018-05-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"81654995","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
The experiment comprising of four levels of phosphorus, i.e., 0, 20, 40 and 60 kg ha-1 and three levels of sulphur, i.e., 0, 15 and 30 kg ha-1, was conducted during summer of 2015-16 to investigate the interaction effect of phosphorus and sulphur application on nutrients uptake and yield of black gram cv. Uttara. The treatments were laid in randomized block design (Factorial) with three replications. The results revealed utmost concentration and uptake of phosphorus in seed (0.376% and 3.59 kg ha-1) and stover (0.266% and 6.38 kg ha-1) and sulphur in seed (0.397% and 3.79 kg ha-1) and stover (0.134% and 3.21 kg ha-1) with combined application of phosphorus 60 kg and sulphur 30 kg ha-1, indicating synergistic effect of phosphorus and sulphur on nutrient uptake, respectively. The results also revealed that combined application of phosphorus and sulphur showed synergistic effect on seed and stover yield of black gram with increasing levels of phosphorus and sulphur up to highest level as both the nutrients mutually help absorption and utilization by black gram probably due to balanced nutrition. The seed and stover yield were 955.50 and 2398.30 kg ha-1 with combined application of phosphorus 60 kg and sulphur 30 kg ha-1. The yield attributes of black gram viz., plant height, number of pods plant-1 and 100 seeds weight also increased significantly with increasing levels of phosphorus and sulphur up to highest level and the optimum values were recorded with combined application of phosphorus 60 kg and sulphur 30 kg ha-1. However, non-significant response of phosphorus and sulphur application has been observed in case of plant population (m-2).
本试验于2015-16年夏季采用0、20、40和60 kg ha-1 4个磷水平和0、15和30 kg ha-1 3个硫水平，研究磷和硫对黑克cv养分吸收和产量的互作效应。Uttara。处理采用随机区组设计(阶乘)，3个重复。结果表明，当磷和硫分别为60 kg和30 kg ha-1时，种子和秸秆中磷(分别为0.376%和3.59 kg ha-1)和硫(分别为0.266%和6.38 kg ha-1)的浓度和吸收量最大，种子和秸秆中硫(分别为0.397%和3.79 kg ha-1)和硫(分别为0.134%和3.21 kg ha-1)的吸收量最大，表明磷和硫对养分吸收具有协同效应。结果还表明，磷硫配施对黑克种子和秸秆产量具有协同效应，磷硫配施水平提高至最高水平，这可能是由于营养平衡导致的两种养分相互促进黑克的吸收和利用。在磷60 kg、硫30 kg hm -1配施条件下，种子和秸秆产量分别为955.50和2398.30 kg hm -1。随着磷和硫用量的增加，黑克重、株高、荚果数、百粒重等产量性状均显著增加，磷和硫配施量达到最高水平，以磷60 kg、硫30 kg hm -1为最佳。然而，在植物种群(m-2)的情况下，磷和硫的施用没有显著的响应。
{"title":"Interaction effect of phosphosrus and sulphur application on nutrient uptake, yield and yield attributing parameters of black gram [Vigna mungo (L.) Hepper]","authors":"M. Phogat, Ankush Rai, Sunil Kumar","doi":"10.18805/LR-3963","DOIUrl":"https://doi.org/10.18805/LR-3963","url":null,"abstract":"The experiment comprising of four levels of phosphorus, i.e., 0, 20, 40 and 60 kg ha-1 and three levels of sulphur, i.e., 0, 15 and 30 kg ha-1, was conducted during summer of 2015-16 to investigate the interaction effect of phosphorus and sulphur application on nutrients uptake and yield of black gram cv. Uttara. The treatments were laid in randomized block design (Factorial) with three replications. The results revealed utmost concentration and uptake of phosphorus in seed (0.376% and 3.59 kg ha-1) and stover (0.266% and 6.38 kg ha-1) and sulphur in seed (0.397% and 3.79 kg ha-1) and stover (0.134% and 3.21 kg ha-1) with combined application of phosphorus 60 kg and sulphur 30 kg ha-1, indicating synergistic effect of phosphorus and sulphur on nutrient uptake, respectively. The results also revealed that combined application of phosphorus and sulphur showed synergistic effect on seed and stover yield of black gram with increasing levels of phosphorus and sulphur up to highest level as both the nutrients mutually help absorption and utilization by black gram probably due to balanced nutrition. The seed and stover yield were 955.50 and 2398.30 kg ha-1 with combined application of phosphorus 60 kg and sulphur 30 kg ha-1. The yield attributes of black gram viz., plant height, number of pods plant-1 and 100 seeds weight also increased significantly with increasing levels of phosphorus and sulphur up to highest level and the optimum values were recorded with combined application of phosphorus 60 kg and sulphur 30 kg ha-1. However, non-significant response of phosphorus and sulphur application has been observed in case of plant population (m-2).","PeriodicalId":18181,"journal":{"name":"Legume Research","volume":null,"pages":null},"PeriodicalIF":0.8,"publicationDate":"2018-05-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"82044322","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
M. Ahmad, M. Agnihotri, M. S. Khan, A. Dubey, Bhawna Tyagi, Rajesh Kumar, N. Prakash
Eleven chickpea varieties were screened for their biochemical resistance to the pulse beetle (Callosobruchus chinensis L.), a serious pest of the stored pulses. The varieties were found to arrest the growth and development of C. chinensis, at grub stages which were indicated by different parameters viz., oviposition, adult emergence, weight loss, developmental period and growth index. Among the various biochemical analyzed, high growth index was observed in the varieties PKG 2 (0.61), BG 1003 (0.62), BG 1053 (0.62) and PKG 1 (0.71). Low growth index recorded in PG 3 (0.52), BGM 547 and PG 186 (0.56) may be attributed to low phenol and tannin content. Similarly the varieties PKG 1, BG 1003 and BG 1053 with less phenol, flavonoids and tannin content recorded more growth index as compared to moderate resistance varieties PG 4, PBG 1 and PG 114 PBG 1, BGM 547 and PG 114 were found to be moderately resistant and PKG 1, PKG 2, BG 1053 and BG 1003 as highly susceptible, shows the major role of trypsin inhibitor in protein resistance to C. chinensis. The highest protease activity inhibition acts as antimetabolites to C. chinensis, inhibit to the feeding of grubs as result higher trypsin content varieties showed relative resistance. The correlation between different antinutritional factors and growth index of the grub also showed a negative relationship.
{"title":"Biochemical basis of resistance in chickpea varieties against Callosobruchus chinensis Linn. (Coleoptera: Bruchidae)","authors":"M. Ahmad, M. Agnihotri, M. S. Khan, A. Dubey, Bhawna Tyagi, Rajesh Kumar, N. Prakash","doi":"10.18805/LR-3848","DOIUrl":"https://doi.org/10.18805/LR-3848","url":null,"abstract":"Eleven chickpea varieties were screened for their biochemical resistance to the pulse beetle (Callosobruchus chinensis L.), a serious pest of the stored pulses. The varieties were found to arrest the growth and development of C. chinensis, at grub stages which were indicated by different parameters viz., oviposition, adult emergence, weight loss, developmental period and growth index. Among the various biochemical analyzed, high growth index was observed in the varieties PKG 2 (0.61), BG 1003 (0.62), BG 1053 (0.62) and PKG 1 (0.71). Low growth index recorded in PG 3 (0.52), BGM 547 and PG 186 (0.56) may be attributed to low phenol and tannin content. Similarly the varieties PKG 1, BG 1003 and BG 1053 with less phenol, flavonoids and tannin content recorded more growth index as compared to moderate resistance varieties PG 4, PBG 1 and PG 114 PBG 1, BGM 547 and PG 114 were found to be moderately resistant and PKG 1, PKG 2, BG 1053 and BG 1003 as highly susceptible, shows the major role of trypsin inhibitor in protein resistance to C. chinensis. The highest protease activity inhibition acts as antimetabolites to C. chinensis, inhibit to the feeding of grubs as result higher trypsin content varieties showed relative resistance. The correlation between different antinutritional factors and growth index of the grub also showed a negative relationship.","PeriodicalId":18181,"journal":{"name":"Legume Research","volume":null,"pages":null},"PeriodicalIF":0.8,"publicationDate":"2018-05-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"72404960","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Field experiments were conducted for two successive years with grass pea (cv. ‘Prateek’) sown on nine dates at weekly interval at Instructional Farm (22°58´ N, 88°31´ E), Bidhan Chandra Krishi Viswavidyalaya, West Bengal, India to investigate the impact of agroclimatic factors on pod filling of grass pea. Results showed that pod filling percentage (PFP) increased with delay in sowing dates, attaining the highest value (96.7 %) in crop sown on 30th November, beyond which it decreased gradually with further delay in sowing. Maximum and minimum temperatures, morning and afternoon soil temperatures, recorded at 5, 15 and 30 cm soil depths, morning and afternoon vapour pressure deficits at pre-flowering phase exhibited negative association and contrarily, when prevailing during reproductive and pod development phases, these parameters demonstrated positive correlation with PFP. Temperature range during reproductive phase increased with delay in sowing dates and it exhibited significant positive correlation with PFP. As demonstrated by stepwise regression analysis, Accumulated photothermal unit (APTU) prevailing at maturity phase alone accounted for 67.4 % of the total variation in PFP and together with temperature range it explained 91.3 % variation. APTU, temperature range, afternoon vapour pressure deficit and afternoon soil temperature seem to be the critical agroclimatic variables influencing the pod filling percentage significantly.
{"title":"Assessing the impact of agro-climatic factors on pod filling of grass pea","authors":"Argha Ghosh","doi":"10.18805/LR-4008","DOIUrl":"https://doi.org/10.18805/LR-4008","url":null,"abstract":"Field experiments were conducted for two successive years with grass pea (cv. ‘Prateek’) sown on nine dates at weekly interval at Instructional Farm (22°58´ N, 88°31´ E), Bidhan Chandra Krishi Viswavidyalaya, West Bengal, India to investigate the impact of agroclimatic factors on pod filling of grass pea. Results showed that pod filling percentage (PFP) increased with delay in sowing dates, attaining the highest value (96.7 %) in crop sown on 30th November, beyond which it decreased gradually with further delay in sowing. Maximum and minimum temperatures, morning and afternoon soil temperatures, recorded at 5, 15 and 30 cm soil depths, morning and afternoon vapour pressure deficits at pre-flowering phase exhibited negative association and contrarily, when prevailing during reproductive and pod development phases, these parameters demonstrated positive correlation with PFP. Temperature range during reproductive phase increased with delay in sowing dates and it exhibited significant positive correlation with PFP. As demonstrated by stepwise regression analysis, Accumulated photothermal unit (APTU) prevailing at maturity phase alone accounted for 67.4 % of the total variation in PFP and together with temperature range it explained 91.3 % variation. APTU, temperature range, afternoon vapour pressure deficit and afternoon soil temperature seem to be the critical agroclimatic variables influencing the pod filling percentage significantly.","PeriodicalId":18181,"journal":{"name":"Legume Research","volume":null,"pages":null},"PeriodicalIF":0.8,"publicationDate":"2018-05-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"80208560","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
A. Malik, K. Sehrawat, Anil.k. Ahlawat, A. R. Sehrawat
Medicinal plants are of great interest in the field of biotechnology as most of the drug industries depend in part on plants for production of pharmaceutical compounds. Biological activities of the phyoconstituents in plants could be enhanced by manipulation of the culture conditions. In the present investigation leaves and nodal segments of Alhagi maurorum were cultured on MS medium with BAP, Kinetin, NAA and other adjuvants. MS medium with BAP (1.0 and 2.0 mg/l) alone and in combination with NAA (0.1 mg/l was good for initiation of calli and bud formation. BAP (2.0 and 5.0mg/l) with NAA(0.1mg/l) plus kinetin 0.25mg/l with ascorbic acid 50mg/l and adenine sulphate, citric acid and arginine 25mg/l each was found to be the best for bud proliferation and shoot multiplication. Best rooting was found on MS medium supplemented with IAA and IBA (0.5mg/ leach). Cultured materials at different growth phase were evaluated for their biochemical estimation of primary metabolites quantitatively. Maximum content of carbohydrates, total proteins and amino acids were noticed in leaves of field grown plants and shoots of regenerated plantlets. It is further concluded that Alhagi maurorum serve as a rich source of primary metabolites which can be used as raw materials in industry.
{"title":"A comparative biochemical evaluation of in vivo and in vitro propagated Alhagi maurorum: an important medicinal plant","authors":"A. Malik, K. Sehrawat, Anil.k. Ahlawat, A. R. Sehrawat","doi":"10.18805/LR-4016","DOIUrl":"https://doi.org/10.18805/LR-4016","url":null,"abstract":"Medicinal plants are of great interest in the field of biotechnology as most of the drug industries depend in part on plants for production of pharmaceutical compounds. Biological activities of the phyoconstituents in plants could be enhanced by manipulation of the culture conditions. In the present investigation leaves and nodal segments of Alhagi maurorum were cultured on MS medium with BAP, Kinetin, NAA and other adjuvants. MS medium with BAP (1.0 and 2.0 mg/l) alone and in combination with NAA (0.1 mg/l was good for initiation of calli and bud formation. BAP (2.0 and 5.0mg/l) with NAA(0.1mg/l) plus kinetin 0.25mg/l with ascorbic acid 50mg/l and adenine sulphate, citric acid and arginine 25mg/l each was found to be the best for bud proliferation and shoot multiplication. Best rooting was found on MS medium supplemented with IAA and IBA (0.5mg/ leach). Cultured materials at different growth phase were evaluated for their biochemical estimation of primary metabolites quantitatively. Maximum content of carbohydrates, total proteins and amino acids were noticed in leaves of field grown plants and shoots of regenerated plantlets. It is further concluded that Alhagi maurorum serve as a rich source of primary metabolites which can be used as raw materials in industry.","PeriodicalId":18181,"journal":{"name":"Legume Research","volume":null,"pages":null},"PeriodicalIF":0.8,"publicationDate":"2018-05-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"75494871","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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