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Impact of sodium and iron levels on yield, nutrient content and uptake by cowpea (Vigna unguiculata L.) 钠和铁水平对豇豆产量、养分含量及吸收的影响
IF 0.8 4区 生物学 Q3 AGRONOMY Pub Date : 2018-07-31 DOI: 10.18805/LR-4004
Kiran Doodhwal, B. Yadav, M. Yadav, M. Choudhary
A pot experiment was conducted to see the effect of sodicity and iron levels on cowpea. Four levels of sodium adsorption ratio (SAR) 10, 15, 20, 25 and iron (Fe) control, 5, 10, 15 and 20 kg Fe ha-1) were tested in completely randomized design with three replications. The results indicated that application of soil sodicity having SAR 15 recorded the maximum yield, phosphorus and potassium content and uptake and nitrogen uptake of cowpea over rest of the treatments. Application of Fe 20 kg ha-1 recorded the maximum and significantly higher yield, nitrogen, phosphorus and potassium content and uptake of cowpea over rest of the treatments. Among the treatment combinations combined application of SAR 15 with Fe @ +20 FeSo4.7H2O) proved superior in all these parameters over other treatments.
通过盆栽试验,研究了不同碱度和铁含量对豇豆生长的影响。采用完全随机设计,3个重复,试验4个水平的钠吸附比(SAR)分别为10、15、20、25和铁(Fe)对照(5、10、15和20 kg Fe ha-1)。结果表明,施用sa15土壤碱度的豇豆产量、磷、钾含量、吸收量和氮素吸收量均高于其他处理。施用Fe 20 kg hm -1处理豇豆产量、氮、磷、钾含量和吸收量最高且显著高于其他处理。在处理组合中,SAR 15与Fe @ +20 (FeSo4.7H2O)的联合应用在所有这些参数上都优于其他处理。
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引用次数: 0
Diversity analysis by SSR markers and morphological markers among Stemphylium blight tolerance genotypes with high yielding cultivars of Lentil (Lens Culinaris Medik) 小扁豆高产品种抗枯萎病基因型的SSR标记和形态标记多样性分析
IF 0.8 4区 生物学 Q3 AGRONOMY Pub Date : 2018-07-31 DOI: 10.18805/LR-4029
D. Mandal, P. Bhattacharyya, R. Das, S. Bhattacharyya
Twelve Simple Sequence Repeat (SSR) markers along with twelve morphological markers were used to assess the diversity among thirteen screened genotypes both moderate resistant and susceptible against stemphylium blight of indigenous microsperma germplasm (i.e. pilosae) type of Lentil (Lens culinaris Medik.) at BCKV, West Bengal. Polymorphic Information Content (PIC) of SSR primers ranged from 0.77 (SSR 317-2) to 0.15 (PBA LC 0233). The maximum Jaccard’s similarity was recorded between L-4769 and Ranjan (approximately 100%) whereas minimum was between K-75 and VL 126 (0.11). Genotypes K-75 also showed less similarity with DL 14-2 and VL 151 (0.17). Another popular variety WBL 77 showed minimum similarity with moderately resistant genotypes VL 151 and DL 14-2 (0.17). While using morphological characters as the basis of diversity analysis, Euclidean coefficient revealed that high yielding cultivar Subrata was maximum diverse with genotype VL 151(7.12). Asha also showed diversity with VL 151(6.31) whereas VL 151 had dissimilarity coefficient of 6.21 with K-75. Precoz and VL 126 has dissimilarity coefficient of 6.19. On the basis of above findings it could be said that diverse parental pairs VL 151 and K 75 and VL 151 and Subrata / Asha could be used in hybridization programme or biotic stress breeding programme for developing Stemphylium resistant lentil cultivars in future.
利用12个SSR标记和12个形态标记,对西孟加拉邦BCKV本地小精子种质(即毛囊)扁豆(Lens culinaris Medik.)中抗性和易感基因型的多样性进行了评价。SSR引物的多态性信息含量(PIC)范围为0.77 (SSR 317-2) ~ 0.15 (PBA LC 0233)。L-4769与Ranjan的Jaccard相似性最高(接近100%),K-75与VL 126的Jaccard相似性最低(0.11)。基因型K-75与DL 14-2和VL 151的相似性也较低(0.17)。另一个流行品种WBL 77与中抗性基因型VL 151和DL 14-2的相似性最小(0.17)。以形态特征作为多样性分析的基础,欧几里得系数显示,高产品种Subrata的多样性最大,基因型为VL 151(7.12)。Asha与VL 151的差异系数为6.31,而VL 151与K-75的差异系数为6.21。Precoz与VL 126的差异系数为6.19。综上所述,VL 151和k75亲本组合以及VL 151和Subrata / Asha亲本组合可用于杂交或生物胁迫育种,以培育抗茎蚜扁豆品种。
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引用次数: 1
Identification and characterization of microsatellite markers in fenugreek: An inter-family amplification 胡芦巴微卫星标记的鉴定与鉴定:家族间扩增
IF 0.8 4区 生物学 Q3 AGRONOMY Pub Date : 2018-07-31 DOI: 10.18805/LR-4024
G. Jethra, S. Choudhary, V. Sharma
Fenugreek (Trigonella foenum-graecum) is one of the most important and primitive medicinal plants belonging to family fabaceae and is recognized as one of the most prominent seed spices. Development, identification and classification of entirely new set of SSRs are a very expensive and time consuming process for a crop like fenugreek. Instead, a novel set of SSRs can be achieved with ease in less expense by transferring the microsatellite loci of the related species in fenugreek. SSR’s of two major crops, carrot and celery belonging to apiaceae family are available in public domain for cross-species and inter-family investigation. In the present study, we examined transferability of 100 carrot SSR loci in fenugreek of which 19% of SSR’s amplified, where 5 primers were monomorphic. 14 SSRs were polymorphic in nature and the bands ranged between 2 to 5. GSSR-87, GSSR-92, GSSR-136 and BSSR-8 showed monomorphic bands and were specific for fenugreek genotypes and can be regarded as a dominant tool for fenugreek breeding and genetic studies. However, GSSR-81 and GSSR-96 were most polymorphic in nature and can be utilized further to distinguish fenugreek from other seed spices. The analysis, revealed optimum level of cross-species transferability (>35.2%) in fenugreek, where no SSR resource is available till date, initiating cross transferability testing from the enriched SSR database of carrot.
葫芦巴(Trigonella foenum-graecum)是豆科最重要的原始药用植物之一,是公认的最重要的种子香料之一。对于胡芦巴这样的作物来说,开发、鉴定和分类全新的SSRs是一个非常昂贵和耗时的过程。相反,通过转移葫芦巴中相关物种的微卫星位点,可以以更低的成本轻松地获得一组新的SSRs。作为apiaceae科的两种主要作物,胡萝卜和芹菜的SSR已在公共领域公开,可用于跨种和科间调查。在本研究中,我们检测了100个胡萝卜SSR位点在葫芦巴中的可转移性,其中19%的SSR位点扩增,其中5个引物为单态引物。14个SSRs具有多态性,条带范围在2 ~ 5之间。GSSR-87、GSSR-92、GSSR-136和BSSR-8为单态带,特异于胡芦巴基因型,可作为胡芦巴育种和遗传研究的优势工具。然而,GSSR-81和GSSR-96在自然界中是最具多态性的,可以进一步用来区分胡芦巴和其他种子香料。分析结果表明,胡芦巴的跨种可转移性达到最佳水平(>35.2%),而胡芦巴目前还没有SSR资源,因此开始从胡萝卜丰富的SSR数据库中进行跨种可转移性测试。
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引用次数: 1
Impact of Long-term fertilizer application on soil fertility, nutrient uptake, growth and productivity of soybean under soybean-safflower cropping sequence in Vertisol 大豆-红花种植顺序下长期施肥对土壤肥力、养分吸收、大豆生长和生产力的影响
IF 0.8 4区 生物学 Q3 AGRONOMY Pub Date : 2018-07-31 DOI: 10.18805/LR-3676
N. Meshram, S. Ismail, S. Shirale, V. D. Patil
An experiment conducted during 2011-12 and 2012-13 under the ongoing All India Coordinated Research Project on Long-Term Fertilizer Experiment with soybean-safflower cropping sequence in Vertisol (Typic haplusterts) that commenced form 2006-07 at Vasantrao Naik Marathwada Krishi Vidyapeeth, Parbhani (M.S.) showed that the nutrients availability in soil, nutrient uptake, yield attributes and yield of soybean were significantly influenced by FYM along with 100% NPK application. The results revealed that the pods per plant (48.65), plant height (49.77 cm plant-1), grain (26.97 q ha-1) and straw (24.65 q ha-1) yield of soybean were significantly increased with 100% NPK+FYM@ 5 Mg ha-1. Conjoint use of FYM with 100% NPK improved the soil properties with respect to bulk density, organic carbon, exchangeable (Ca, Mg, Na & K), cation exchange capacity (CEC), base saturation (BS), available N, P, K, S, Zn and Fe over initial value of soil. However, a significant buildup of available Zn due to the application of zinc sulphate along with 100% NPK as compared to all other treatments. N, P, K, S, Zn and Fe uptake of soybean were increased significantly with the application of 100 % NPK+FYM@ 5 Mg ha 1 than other treatment. It can be concluded from these experiment that the balanced use of fertilizers continuously either alone or in combination with organic manure is necessary for sustaining soil fertility and productivity of crop.
2011-12年和2012-13年,在正在进行的全印度协调研究项目下,在2006-07年开始在Vasantrao Naik Marathwada Krishi Vidyapeeth, Parbhani (ms)进行的大豆-红花长期施肥试验(典型haplusterts)中进行的一项试验表明,FYM和100% NPK施用对土壤养分有效性、养分吸收、产量属性和大豆产量都有显著影响。结果表明,100% NPK+ fym@5mg ha-1处理显著提高了大豆单株荚果(48.65个)、株高(49.77 cm)、籽粒(26.97 q ha-1)和秸秆(24.65 q ha-1)产量。与初始值相比,FYM与100% NPK联合施用改善了土壤的容重、有机碳、交换性(Ca、Mg、Na和K)、阳离子交换容量(CEC)、碱饱和度(BS)、有效N、P、K、S、Zn和Fe。然而,与所有其他处理相比,硫酸锌与100%氮磷钾的施用显著增加了有效锌。100% NPK+FYM@ 5 Mg ha 1处理显著提高了大豆对N、P、K、S、Zn和Fe的吸收。从这些试验中可以得出结论,化肥的连续平衡使用,无论是单独使用还是与有机肥结合使用,都是保持土壤肥力和作物生产力的必要条件。
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引用次数: 2
Performance of cowpea varieties under zero tillage conditions in rice-wheat cropping system 水稻-小麦免耕条件下豇豆品种的生产性能
IF 0.8 4区 生物学 Q3 AGRONOMY Pub Date : 2018-05-24 DOI: 10.18805/LR-3972
D. Mal, S. Datta, Pranali Tulsidas Bhaisare, R. Chatterjee, A. Chowdhury, J. C. Jana
The present experimentation was undertaken during pre-kharif season of 2013 and 2014 to study the varietal performance of cowpea in rice-wheat cropping system at Uttar Banga Krishi Viswavidyalaya, Pundibai, Cooch Behar, West Bengal after harvesting of wheat under zero tillage conditions and before rice sowing. Experiment was laid out in randomized block design with three replications by using eighteen varieties of cowpea. The result of pooled data revealed that all the growth, yield parameters and quality parameters varied significantly with respect to different varieties of cowpea. The longest and lowest vine length was noticed in Lafa Sundari Bangla (112.10 cm) and VU-5 (32.64 cm), minimum and maximum days for flowering, first harvesting and last harvesting was recorded in Kaberee and Pusa Phalguni Gold. Variety Girija and Deshi Lafa produced longest pod (45.40 cm), highest individual pod weight (14.29g). For number of pods per plant Triguna (31.07) was found highest which was statistically at par with Kanak (30.70), Kashi Kanchan (29.33). Maximum yield of 18.51 t/ha was obtained from Kashi Kanchan followed by Triguna (17.07 tonnes/ ha) and Kanak (16.69 tonnes/ha). Lafa Sundari Bangala recorded with highest beta carotene content (1037 IU/100g fresh pod), ascorbic acid content (20.17 mg/100g fresh pod) as well as highest protein content of 4.69 per cent. Hence, it is concluded that the cowpea variety Kashi Kanchan, Triguna and Kanak may be recommended for commercial cultivation in rice-wheat cropping system under terai zone of West Bengal as they produced higher yield (34.52 %, 24.06 % and 21.29 %, respectively) over the check variety Lafa Sundari Bangla.
本试验于2013年和2014年收割前季节进行,旨在研究西孟加拉邦Pundibai、Cooch Behar、Uttar Banga Krishi Viswavidyalaya水稻-小麦种植系统中豇豆在免耕条件下收获小麦后和水稻播种前的品种表现。试验采用随机区组设计,3个重复,选用18个豇豆品种。汇总数据表明,不同品种豇豆的生长、产量和品质参数均存在显著差异。最长和最短的藤长分别记录在Lafa Sundari Bangla (112.10 cm)和VU-5 (32.64 cm),最短和最长的开花天数,第一次收获和最后一次收获记录在Kaberee和Pusa Phalguni Gold。品种“吉丽佳”和“德施拉法”的荚果最长(45.40厘米),单荚重最高(14.29克)。单株荚果数以Triguna(31.07)最高,与Kanak(30.70)、Kashi kan禅(29.33)持平。喀什干禅的最高产量为18.51吨/公顷,其次是特里古纳(17.07吨/公顷)和卡纳克(16.69吨/公顷)。其中-胡萝卜素含量最高(1037 IU/100g新鲜豆荚),抗坏血酸含量最高(20.17 mg/100g新鲜豆荚),蛋白质含量最高(4.69%)。因此,由于豇豆品种Kashi Kanchan, Triguna和Kanak的产量分别比对照品种Lafa Sundari Bangla高34.52%,24.06%和21.29%,因此可以推荐在西孟加拉邦terai地区的稻麦种植系统中进行商业种植。
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引用次数: 1
Effect of sowing dates, mulching and seed rates on nutrient uptake and productivity of soybean in sub - humid Punjab, India 印度旁遮普省半湿润地区播期、覆盖和播种量对大豆养分吸收和生产力的影响
IF 0.8 4区 生物学 Q3 AGRONOMY Pub Date : 2018-05-20 DOI: 10.18805/LR-3731
R. Sikka, J. Deol, Jagmohan Kaur, D. Singh
A field experiment was conducted for three years to study the effect of sowing dates, mulching and seed rates on nutrient uptake and productivity of soybean under irrigated conditions in sub-humid Punjab on a silty clay loam soil which was low in available N, medium in available P and available K. The experiment was conducted in a split plot design with the combination of three sowing dates and mulch in main plots and three seed rates in sub-plots with three replications on fixed site. The results revealed that sowing on June 30 and mulching recorded the highest number of pods plant-1.There was no significant influence of seed rates on yield attributes. Seed yield also remained at par under different sowing dates, mulching and seed rate treatments. Stover yield however, was significantly higher under the earlier sowing dates and mulching. Highest total N uptake was observed under June 30 sowing date whereas total P and K uptake was highest in May 20 sowing date. No significant effect of mulching was observed on total N and K uptake. Among seed rates maximum total N uptake was observed under 75 kg seed ha-1. The results suggest that soybean performed better when sown with 60 kg seed rate ha-1 on June 30 and mulched with wheat straw.
进行现场试验了三年学习的影响播种日期、覆盖和种子利率在灌溉条件下大豆的营养吸收和生产力在半湿润旁遮普粉砂质粘壤土土在可用低N,可用介质P和k .可用实验是在分裂阴谋进行设计组合的三个播种日期和地膜主要情节插曲和三个种子率三个固定的网站上复制。结果表明,6月30日播种和复盖时植株1号的荚果数最高。籽粒率对产量性状无显著影响。不同播期、覆盖和种子量处理的种子产量基本相同。然而,较早播种和覆盖的秸秆产量显著较高。6月30日播期全氮吸收量最高,5月20日播期全磷、全钾吸收量最高。覆盖对全氮和全钾吸收无显著影响。各种子速率下,总氮吸收量在75 kg种子hm -1时最大。结果表明,6月30日播种60 kg播种率hm -1,覆盖麦秸时,大豆表现较好。
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引用次数: 3
Interaction effect of phosphosrus and sulphur application on nutrient uptake, yield and yield attributing parameters of black gram [Vigna mungo (L.) Hepper] 施磷加硫对黑克兰养分吸收、产量及产量属性参数的互作效应玫瑰)
IF 0.8 4区 生物学 Q3 AGRONOMY Pub Date : 2018-05-20 DOI: 10.18805/LR-3963
M. Phogat, Ankush Rai, Sunil Kumar
The experiment comprising of four levels of phosphorus, i.e., 0, 20, 40 and 60 kg ha-1 and three levels of sulphur, i.e., 0, 15 and 30 kg ha-1, was conducted during summer of 2015-16 to investigate the interaction effect of phosphorus and sulphur application on nutrients uptake and yield of black gram cv. Uttara. The treatments were laid in randomized block design (Factorial) with three replications. The results revealed utmost concentration and uptake of phosphorus in seed (0.376% and 3.59 kg ha-1) and stover (0.266% and 6.38 kg ha-1) and sulphur in seed (0.397% and 3.79 kg ha-1) and stover (0.134% and 3.21 kg ha-1) with combined application of phosphorus 60 kg and sulphur 30 kg ha-1, indicating synergistic effect of phosphorus and sulphur on nutrient uptake, respectively. The results also revealed that combined application of phosphorus and sulphur showed synergistic effect on seed and stover yield of black gram with increasing levels of phosphorus and sulphur up to highest level as both the nutrients mutually help absorption and utilization by black gram probably due to balanced nutrition. The seed and stover yield were 955.50 and 2398.30 kg ha-1 with combined application of phosphorus 60 kg and sulphur 30 kg ha-1. The yield attributes of black gram viz., plant height, number of pods plant-1 and 100 seeds weight also increased significantly with increasing levels of phosphorus and sulphur up to highest level and the optimum values were recorded with combined application of phosphorus 60 kg and sulphur 30 kg ha-1. However, non-significant response of phosphorus and sulphur application has been observed in case of plant population (m-2).
本试验于2015-16年夏季采用0、20、40和60 kg ha-1 4个磷水平和0、15和30 kg ha-1 3个硫水平,研究磷和硫对黑克cv养分吸收和产量的互作效应。Uttara。处理采用随机区组设计(阶乘),3个重复。结果表明,当磷和硫分别为60 kg和30 kg ha-1时,种子和秸秆中磷(分别为0.376%和3.59 kg ha-1)和硫(分别为0.266%和6.38 kg ha-1)的浓度和吸收量最大,种子和秸秆中硫(分别为0.397%和3.79 kg ha-1)和硫(分别为0.134%和3.21 kg ha-1)的吸收量最大,表明磷和硫对养分吸收具有协同效应。结果还表明,磷硫配施对黑克种子和秸秆产量具有协同效应,磷硫配施水平提高至最高水平,这可能是由于营养平衡导致的两种养分相互促进黑克的吸收和利用。在磷60 kg、硫30 kg hm -1配施条件下,种子和秸秆产量分别为955.50和2398.30 kg hm -1。随着磷和硫用量的增加,黑克重、株高、荚果数、百粒重等产量性状均显著增加,磷和硫配施量达到最高水平,以磷60 kg、硫30 kg hm -1为最佳。然而,在植物种群(m-2)的情况下,磷和硫的施用没有显著的响应。
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引用次数: 2
Assessing the impact of agro-climatic factors on pod filling of grass pea 农业气候因子对豆荚灌浆的影响评价
IF 0.8 4区 生物学 Q3 AGRONOMY Pub Date : 2018-05-20 DOI: 10.18805/LR-4008
Argha Ghosh
Field experiments were conducted for two successive years with grass pea (cv. ‘Prateek’) sown on nine dates at weekly interval at Instructional Farm (22°58´ N, 88°31´ E), Bidhan Chandra Krishi Viswavidyalaya, West Bengal, India to investigate the impact of agroclimatic factors on pod filling of grass pea. Results showed that pod filling percentage (PFP) increased with delay in sowing dates, attaining the highest value (96.7 %) in crop sown on 30th November, beyond which it decreased gradually with further delay in sowing. Maximum and minimum temperatures, morning and afternoon soil temperatures, recorded at 5, 15 and 30 cm soil depths, morning and afternoon vapour pressure deficits at pre-flowering phase exhibited negative association and contrarily, when prevailing during reproductive and pod development phases, these parameters demonstrated positive correlation with PFP. Temperature range during reproductive phase increased with delay in sowing dates and it exhibited significant positive correlation with PFP. As demonstrated by stepwise regression analysis, Accumulated photothermal unit (APTU) prevailing at maturity phase alone accounted for 67.4 % of the total variation in PFP and together with temperature range it explained 91.3 % variation. APTU, temperature range, afternoon vapour pressure deficit and afternoon soil temperature seem to be the critical agroclimatic variables influencing the pod filling percentage significantly.
以草豆为原料,连续两年进行田间试验。“Prateek”)在印度西孟加拉邦Bidhan Chandra Krishi Viswavidyalaya的教学农场(22°58´N, 88°31´E),每周间隔9天播种,以研究农业气候因素对草豌豆荚的影响。结果表明,籽粒结实率(PFP)随播期的推迟而增加,在11月30日播种时达到最高值(96.7%),之后随播期的推迟而逐渐降低。在5、15和30 cm土壤深度记录的最高和最低温度、上午和下午土壤温度、花前期的上午和下午蒸汽压差与PFP呈负相关,而在繁殖和荚果发育阶段盛行时,这些参数与PFP呈正相关。生殖期温度变化幅度随播期的推迟而增大,与PFP呈显著正相关。逐步回归分析表明,成熟期累积光热单位(APTU)占PFP总变异的67.4%,与温度差一起占91.3%。APTU、温度差、下午蒸汽压亏缺和下午土壤温度是影响荚果灌浆率的关键农业气候变量。
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引用次数: 3
Biochemical basis of resistance in chickpea varieties against Callosobruchus chinensis Linn. (Coleoptera: Bruchidae) 鹰嘴豆品种对中国斑蛾抗性的生化基础。(鞘翅目:豆象科)
IF 0.8 4区 生物学 Q3 AGRONOMY Pub Date : 2018-05-20 DOI: 10.18805/LR-3848
M. Ahmad, M. Agnihotri, M. S. Khan, A. Dubey, Bhawna Tyagi, Rajesh Kumar, N. Prakash
Eleven chickpea varieties were screened for their biochemical resistance to the pulse beetle (Callosobruchus chinensis L.), a serious pest of the stored pulses. The varieties were found to arrest the growth and development of C. chinensis, at grub stages which were indicated by different parameters viz., oviposition, adult emergence, weight loss, developmental period and growth index. Among the various biochemical analyzed, high growth index was observed in the varieties PKG 2 (0.61), BG 1003 (0.62), BG 1053 (0.62) and PKG 1 (0.71). Low growth index recorded in PG 3 (0.52), BGM 547 and PG 186 (0.56) may be attributed to low phenol and tannin content. Similarly the varieties PKG 1, BG 1003 and BG 1053 with less phenol, flavonoids and tannin content recorded more growth index as compared to moderate resistance varieties PG 4, PBG 1 and PG 114 PBG 1, BGM 547 and PG 114 were found to be moderately resistant and PKG 1, PKG 2, BG 1053 and BG 1003 as highly susceptible, shows the major role of trypsin inhibitor in protein resistance to C. chinensis. The highest protease activity inhibition acts as antimetabolites to C. chinensis, inhibit to the feeding of grubs as result higher trypsin content varieties showed relative resistance. The correlation between different antinutritional factors and growth index of the grub also showed a negative relationship.
对11个鹰嘴豆品种进行了对脉冲甲虫(Callosobruchus chinensis L.)生化抗性的筛选。结果表明,不同品种在蛴螬期(产卵期、成虫羽化期、体重减轻期、发育期和生长指数)均能抑制蠋蝽的生长发育。在生化分析品种中,PKG 2(0.61)、bg1003(0.62)、bg1053(0.62)和PKG 1(0.71)的生长指数较高。PG 3(0.52)、BGM 547和PG 186(0.56)的生长指数较低,可能是酚和单宁含量较低所致。同样,苯酚、黄酮类和单宁含量较低的品种PKG 1、BG 1003和BG 1053的生长指数高于中抗性品种PKG 4、pbg1和pg114,其中PKG 1、BGM 547和pg114为中抗性品种,PKG 1、PKG 2、BG 1053和BG 1003为高敏感性品种,说明胰蛋白酶抑制剂在对中国紫荆蛋白抗性中的主要作用。蛋白酶活性最高的品种作为抗代谢产物,抑制了蛴螬的摄食,因此胰蛋白酶含量较高的品种表现出相对抗性。不同抗营养因子与蛴螬生长指数呈负相关。
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引用次数: 4
A comparative biochemical evaluation of in vivo and in vitro propagated Alhagi maurorum: an important medicinal plant 重要药用植物毛竹在体内和离体繁殖的比较生化评价
IF 0.8 4区 生物学 Q3 AGRONOMY Pub Date : 2018-05-20 DOI: 10.18805/LR-4016
A. Malik, K. Sehrawat, Anil.k. Ahlawat, A. R. Sehrawat
Medicinal plants are of great interest in the field of biotechnology as most of the drug industries depend in part on plants for production of pharmaceutical compounds. Biological activities of the phyoconstituents in plants could be enhanced by manipulation of the culture conditions. In the present investigation leaves and nodal segments of Alhagi maurorum were cultured on MS medium with BAP, Kinetin, NAA and other adjuvants. MS medium with BAP (1.0 and 2.0 mg/l) alone and in combination with NAA (0.1 mg/l was good for initiation of calli and bud formation. BAP (2.0 and 5.0mg/l) with NAA(0.1mg/l) plus kinetin 0.25mg/l with ascorbic acid 50mg/l and adenine sulphate, citric acid and arginine 25mg/l each was found to be the best for bud proliferation and shoot multiplication. Best rooting was found on MS medium supplemented with IAA and IBA (0.5mg/ leach). Cultured materials at different growth phase were evaluated for their biochemical estimation of primary metabolites quantitatively. Maximum content of carbohydrates, total proteins and amino acids were noticed in leaves of field grown plants and shoots of regenerated plantlets. It is further concluded that Alhagi maurorum serve as a rich source of primary metabolites which can be used as raw materials in industry.
药用植物在生物技术领域引起了极大的兴趣,因为大多数制药工业在一定程度上依赖于植物来生产药用化合物。通过调节培养条件,可以提高植物中各成分的生物活性。本研究采用MS培养基,加入BAP、Kinetin、NAA等佐剂,培养毛毛Alhagi的叶片和节段。单独添加BAP(1.0和2.0 mg/l)和NAA (0.1 mg/l)的MS培养基有利于愈伤组织的发生和芽的形成。BAP(2.0和5.0mg/l) + NAA(0.1mg/l) +动蛋白(0.25mg/l) +抗坏血酸(50mg/l)和硫酸腺嘌呤(25mg/l)、柠檬酸(25mg/l)对芽增殖和芽增殖效果最好。在添加IAA和IBA (0.5mg/ leach)的MS培养基上生根效果最好。对不同生长阶段的培养材料进行了初级代谢物的定量生化评价。田间栽培植株的叶片和再生植株的芽中碳水化合物、总蛋白质和氨基酸含量最高。进一步得出毛藻是一种丰富的初级代谢物来源,可作为工业原料。
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引用次数: 2
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