Simvastatin is used to treat Alzheimer’s disease with impaired cognitive function through blocking of PI3K/Akt signaling to inhibit survival of new neurons and growth of neurons. This study investigated the potential of simvastatin on vascular cognitive impairment (VCI) caused by lacunar cerebral infarction (LCI) in the hippocampus of rats. After establishment of LCI models, rats were administered with NaOH solution (model group), simvastatin (simvastatin group), and PI3K/Akt pathway inhibitor (inhibitor group), respectively, and 10 healthy rats served as control group. Upon treatment, Y-maze and Morris water maze detected spatial cognition ability of rats; while cleaved caspase-3 staining was used to assess apoptosis in the hippocampus. Western blot analysis detected Akt and ERK1/2 phosphorylation levels and enzyme-linked immuno sorbent assay (ELISA) determined the content of brain-derived neurotrophic factor (BDNF). The Y maze Morris water maze confirmed impairment of long-term memory abilities in the LCI rats from the model group. Treatment with simvastatin significantly improved correct rate of arm alternates in rats and shorted the stage latency in the hidden platform experiment. The number of apoptotic cells increased in the hippocampal DG sub-granular area of rats with LCI, and BDNF level was significantly reduced. Administration of simvastatin suppressed cell apoptosis and increased BDNF level when improving phosphorylation level of Akt and ERK1/2 as well. Cerebral ischemia following LCI affects short and long-term memory, which leads to VCI. Simvastatin increases Akt and ERK1 in PI3K/Akt pathway, and hence up-regulates hippocampal BDNF levels, which promotes the regeneration of rat hippocampal nerve cells to relieve VCI.
{"title":"Simvastatin mitigates vascular cognitive impairment in rat’s hippocampus in lacunar cerebral infarction","authors":"Sihan Chen, Yening Zhu, Zhuqin Li, Donger Chen","doi":"10.1166/mex.2024.2612","DOIUrl":"https://doi.org/10.1166/mex.2024.2612","url":null,"abstract":"Simvastatin is used to treat Alzheimer’s disease with impaired cognitive function through blocking of PI3K/Akt signaling to inhibit survival of new neurons and growth of neurons. This study investigated the potential of simvastatin on vascular cognitive impairment (VCI) caused by lacunar cerebral infarction (LCI) in the hippocampus of rats. After establishment of LCI models, rats were administered with NaOH solution (model group), simvastatin (simvastatin group), and PI3K/Akt pathway inhibitor (inhibitor group), respectively, and 10 healthy rats served as control group. Upon treatment, Y-maze and Morris water maze detected spatial cognition ability of rats; while cleaved caspase-3 staining was used to assess apoptosis in the hippocampus. Western blot analysis detected Akt and ERK1/2 phosphorylation levels and enzyme-linked immuno sorbent assay (ELISA) determined the content of brain-derived neurotrophic factor (BDNF). The Y maze Morris water maze confirmed impairment of long-term memory abilities in the LCI rats from the model group. Treatment with simvastatin significantly improved correct rate of arm alternates in rats and shorted the stage latency in the hidden platform experiment. The number of apoptotic cells increased in the hippocampal DG sub-granular area of rats with LCI, and BDNF level was significantly reduced. Administration of simvastatin suppressed cell apoptosis and increased BDNF level when improving phosphorylation level of Akt and ERK1/2 as well. Cerebral ischemia following LCI affects short and long-term memory, which leads to VCI. Simvastatin increases Akt and ERK1 in PI3K/Akt pathway, and hence up-regulates hippocampal BDNF levels, which promotes the regeneration of rat hippocampal nerve cells to relieve VCI.","PeriodicalId":18318,"journal":{"name":"Materials Express","volume":"8 15","pages":""},"PeriodicalIF":0.7,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139125013","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"材料科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
The influence of twins on laser additive CoCrNi MEA was analyzed by experiments and molecular dynamic simulation. Results showed that the traditional laser additive CoCrNi MEA was prone to slip in the (111) direction. EBSD analysis showed an obvious 60°(111} axial angle pair relationship, which is related to the slower cooling rate. The results of molecular dynamic simulation showed that the yield strength of CoCrNi MEA increases first and then decreases, and the material has a critical twin density. When the twin density is less than the critical twin density, the strength of the material increases due to the thinning effect of the twin boundary on the grain. When the twin density is greater than the boundary twin density, the nucleation and proliferation of the twin boundary and the junction of the two become the dominant factors of the material deformation. When the twin density is far from the critical value, the twin spacing becomes smaller, the dislocation sources increase, the dislocation nucleation and proliferation increase, and the strength of the material decreases.
通过实验和分子动力学模拟分析了孪晶对激光添加剂钴铬镍 MEA 的影响。结果表明，传统的激光添加钴铬镍 MEA 在（111）方向上容易发生滑移。EBSD 分析显示出明显的 60°(111} 轴角对关系，这与冷却速度较慢有关。分子动力学模拟结果表明，钴铬镍 MEA 的屈服强度先增大后减小，材料具有临界孪晶密度。当孪晶密度小于临界孪晶密度时，由于孪晶边界对晶粒的减薄作用，材料的强度会增加。当孪晶密度大于边界孪晶密度时，孪晶边界的成核和增殖以及两者的交界成为材料变形的主导因素。 当孪晶密度远离临界值时，孪晶间距变小，位错源增加，位错成核和增殖增加，材料强度降低。
{"title":"Effect of initial twins on mechanical properties of additive manufacturing CoCrNi alloy and molecular dynamic simulation","authors":"Xu Kong, Haiyi Cheng, Yuying Jiang, Di Hu","doi":"10.1166/mex.2024.2593","DOIUrl":"https://doi.org/10.1166/mex.2024.2593","url":null,"abstract":"The influence of twins on laser additive CoCrNi MEA was analyzed by experiments and molecular dynamic simulation. Results showed that the traditional laser additive CoCrNi MEA was prone to slip in the (111) direction. EBSD analysis showed an obvious 60°(111} axial angle pair relationship, which is related to the slower cooling rate. The results of molecular dynamic simulation showed that the yield strength of CoCrNi MEA increases first and then decreases, and the material has a critical twin density. When the twin density is less than the critical twin density, the strength of the material increases due to the thinning effect of the twin boundary on the grain. When the twin density is greater than the boundary twin density, the nucleation and proliferation of the twin boundary and the junction of the two become the dominant factors of the material deformation. When the twin density is far from the critical value, the twin spacing becomes smaller, the dislocation sources increase, the dislocation nucleation and proliferation increase, and the strength of the material decreases.","PeriodicalId":18318,"journal":{"name":"Materials Express","volume":"14 11","pages":""},"PeriodicalIF":0.7,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139126380","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"材料科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
The medicinal value of curcumin has been confirmed in many diseases. This study explores miR-1297’s role in lung cancer cells. The targeting regulation between miR-1297 and BH3-only was analyzed by luciferase activity. Cisplatin (DDP)-resistant cell lines A549/DDP and NCI-H460/DDP were established followed by analysis of cell proliferation activity, BH3-only expression, and cell proliferation by EdU staining. miR-1297 targets BH3-only. miR-1297 expression in A549/DDP and NCI-H460/DDP cells was significantly increased while BH3-only was reduced. Same dose of DDP inhibited the proliferation of A549/DDP and NCI-H460/DDP cells. BH3-only in A549/DDP and NCI-H460/DDP cells in miR-1297 inhibitor transfection group was significantly increased, apoptosis was increased, proliferation was weakened, and drug resistance was reduced. Lung cancer DDP resistance is related to the expression of miR-1297. Down-regulation of miR-1297 can reduce the DDP drug resistance by increasing the expression of BH3-only.
{"title":"Curcumin targets miR-1297 to promote mitochondrial apoptosis and cisplatin sensitization of lung cancer stem cells","authors":"Jiayang Sun, Luzhi Gang, Chunlin Li","doi":"10.1166/mex.2024.2597","DOIUrl":"https://doi.org/10.1166/mex.2024.2597","url":null,"abstract":"The medicinal value of curcumin has been confirmed in many diseases. This study explores miR-1297’s role in lung cancer cells. The targeting regulation between miR-1297 and BH3-only was analyzed by luciferase activity. Cisplatin (DDP)-resistant cell lines A549/DDP and NCI-H460/DDP were established followed by analysis of cell proliferation activity, BH3-only expression, and cell proliferation by EdU staining. miR-1297 targets BH3-only. miR-1297 expression in A549/DDP and NCI-H460/DDP cells was significantly increased while BH3-only was reduced. Same dose of DDP inhibited the proliferation of A549/DDP and NCI-H460/DDP cells. BH3-only in A549/DDP and NCI-H460/DDP cells in miR-1297 inhibitor transfection group was significantly increased, apoptosis was increased, proliferation was weakened, and drug resistance was reduced. Lung cancer DDP resistance is related to the expression of miR-1297. Down-regulation of miR-1297 can reduce the DDP drug resistance by increasing the expression of BH3-only.","PeriodicalId":18318,"journal":{"name":"Materials Express","volume":"14 24","pages":""},"PeriodicalIF":0.7,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139129650","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"材料科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Mycoplasma pneumonia (MPP) belongs to atypical pneumonia, which is caused by Mycoplasma pneumonia infection, accounting for 10–40% of children’s community pneumonia. It is more common in children at preschool age. The clinical symptoms of MPP are varied and difficult to cure. The research analyze the expression of inflammatory factors in MPP, neutrophil/lymphocyte ratio (NLR), insulin-like growth factor 1 (IGF1). The aim is to provide relevant basis for treatment and diagnosis of the disease. 40 normal children were selected as the control group. 78 children with MPP were selected as the observation group. The levels of interleukin-6 (IL-6) and interleukin-2 (IL-2) in peripheral blood were detected. Platelet/lymphocyte ratio (PLR) and NLR were tested. IGF1 expression was determined by ELISA. The correlation between IGF1, IL-6, IL-2NLR, PLR and the severity of MPP was analyzed. IL-2, NLR, and PLR significantly increased. The IGF1 in MPP group was lower than control group (P <0.05). IL-6 level in two group was no significant change. NLR, PLR and IL-2 were negatively correlated with IGF1. NLR, IL-2, PLR were positively correlated with MPP severity (P <0.05). NLR, PLR, and IL-2 were upregulated, whereas IGF1 was declined in MPP, which can be used to evaluate the progression of MPP.
{"title":"Expression analysis of insulin-like growth factor 1, neutrophil/lymphocyte ratio, and inflammatory factors in children with Mycoplasmal pneumonia","authors":"Zhaoyan Wang, Xian Wang, Wanglan Zhao, Jun Luo","doi":"10.1166/mex.2023.2572","DOIUrl":"https://doi.org/10.1166/mex.2023.2572","url":null,"abstract":"Mycoplasma pneumonia (MPP) belongs to atypical pneumonia, which is caused by Mycoplasma pneumonia infection, accounting for 10–40% of children’s community pneumonia. It is more common in children at preschool age. The clinical symptoms of MPP are varied and\u0000 difficult to cure. The research analyze the expression of inflammatory factors in MPP, neutrophil/lymphocyte ratio (NLR), insulin-like growth factor 1 (IGF1). The aim is to provide relevant basis for treatment and diagnosis of the disease. 40 normal children were selected as the control group.\u0000 78 children with MPP were selected as the observation group. The levels of interleukin-6 (IL-6) and interleukin-2 (IL-2) in peripheral blood were detected. Platelet/lymphocyte ratio (PLR) and NLR were tested. IGF1 expression was determined by ELISA. The correlation between IGF1, IL-6, IL-2NLR,\u0000 PLR and the severity of MPP was analyzed. IL-2, NLR, and PLR significantly increased. The IGF1 in MPP group was lower than control group (P <0.05). IL-6 level in two group was no significant change. NLR, PLR and IL-2 were negatively correlated with IGF1. NLR, IL-2, PLR were positively\u0000 correlated with MPP severity (P <0.05). NLR, PLR, and IL-2 were upregulated, whereas IGF1 was declined in MPP, which can be used to evaluate the progression of MPP.","PeriodicalId":18318,"journal":{"name":"Materials Express","volume":" 52","pages":""},"PeriodicalIF":0.7,"publicationDate":"2023-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138612269","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"材料科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Dry eye is a relatively common clinical condition caused by abnormal fluid dynamics and tear quality, for which effective pharmacological treatment is lacking. In this study, a new nano-siRNA delivery system loaded with anti-ESR1 siRNA was designed and synthesized, and its effect in the treatment of dry eye was evaluated. The system significantly inhibited the NaCl-induced increase in inflammatory factors, thereby suppressing the inflammatory response. In addition, the system downregulated the expression level of MMP-9 and STRA6. This system could be developed as a drug for the treatment of diabetes-induced dry eye.
{"title":"Novel nano-SiRNA delivery system loaded with anti-ESR1 siRNA and its application as a cyclosporine drug carrier in dry eye caused by diabetes","authors":"Jing He, Yunzhi Lin, Fen Ye, Ying Gao, Ting Yu, Bilian Ke, Weihong Zhou","doi":"10.1166/mex.2023.2561","DOIUrl":"https://doi.org/10.1166/mex.2023.2561","url":null,"abstract":"Dry eye is a relatively common clinical condition caused by abnormal fluid dynamics and tear quality, for which effective pharmacological treatment is lacking. In this study, a new nano-siRNA delivery system loaded with anti-ESR1 siRNA was designed and synthesized, and its effect in\u0000 the treatment of dry eye was evaluated. The system significantly inhibited the NaCl-induced increase in inflammatory factors, thereby suppressing the inflammatory response. In addition, the system downregulated the expression level of MMP-9 and STRA6. This system could be developed as a drug\u0000 for the treatment of diabetes-induced dry eye.","PeriodicalId":18318,"journal":{"name":"Materials Express","volume":" 31","pages":""},"PeriodicalIF":0.7,"publicationDate":"2023-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138614287","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"材料科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Metformin (MET) is the preferred first-line treatment for patients with type 2 diabetes. However, the mechanism of diabetic peripheral neuropathy (DPN) is still unclear. To improve the oral utilization of metformin, a metformin nanoemulsion (MET-NE) was prepared to investigate its effects on DPN and its underlying mechanism. In this study, a DPN model was established in Wistar rats induced by streptozotocin (STZ). The diabetic rats were randomly divided into four groups: the diabetic model group (DM group), the metformin tablet-treated group (MET group), the metformin nanoemulsion-treated group (MET-NE group), and a normal control group consisting of five normal Wistar rats. All groups were administered the treatment orally for a period of 10 weeks. The findings of the study demonstrated that both MET and MET-NE significantly reduced blood glucose levels, glycated serum protein levels, food intake, and water intake in DM rats. It was also observed that MET-NE was more effective than MET in reducing blood glucose levels. Additionally, both MET and MET-NE treatments significantly increased the motor nerve conduction velocity (MNCV), sensory nerve conduction velocity (SNCV), and sensory nerve action potential (SNAP) amplitude in DM rats. Furthermore, these treatments improved mechanical withdrawal threshold (MWT) and thermal withdrawal latency (TWL), resulting in a reduced sensitivity to pain stimuli. Moreover, both MET and MET-NE treatments promoted the phosphorylation of AMP-activated protein kinase (AMPK) and acetyl-CoA carboxylase (ACC) proteins, as well as increased the activity of autophagic proteins in the sciatic nerve. However, no significant differences were observed between MET and MET-NE treatments in terms of these effects. In conclusion, the MET-NE demonstrated a rapid decrease in blood glucose levels and improved glucose tolerance and metabolism, which was found to be superior to MET. Furthermore, MET-NE significantly improved the neurophysiological function and sciatic nerve pain threshold in DPN rats. These beneficial effects may be attributed to the regulation of AMPK-mediated autophagy by MET-NE.
{"title":"Effect of metformin nanoemulsion through AMP-activated protein kinase (AMPK)-mediated autophagy on diabetic neuropathy","authors":"Fengmin Liu, Siyun Wang, Xiaojuan Du, Diya Xie","doi":"10.1166/mex.2023.2560","DOIUrl":"https://doi.org/10.1166/mex.2023.2560","url":null,"abstract":"Metformin (MET) is the preferred first-line treatment for patients with type 2 diabetes. However, the mechanism of diabetic peripheral neuropathy (DPN) is still unclear. To improve the oral utilization of metformin, a metformin nanoemulsion (MET-NE) was prepared to investigate its effects\u0000 on DPN and its underlying mechanism. In this study, a DPN model was established in Wistar rats induced by streptozotocin (STZ). The diabetic rats were randomly divided into four groups: the diabetic model group (DM group), the metformin tablet-treated group (MET group), the metformin nanoemulsion-treated\u0000 group (MET-NE group), and a normal control group consisting of five normal Wistar rats. All groups were administered the treatment orally for a period of 10 weeks. The findings of the study demonstrated that both MET and MET-NE significantly reduced blood glucose levels, glycated serum protein\u0000 levels, food intake, and water intake in DM rats. It was also observed that MET-NE was more effective than MET in reducing blood glucose levels. Additionally, both MET and MET-NE treatments significantly increased the motor nerve conduction velocity (MNCV), sensory nerve conduction velocity\u0000 (SNCV), and sensory nerve action potential (SNAP) amplitude in DM rats. Furthermore, these treatments improved mechanical withdrawal threshold (MWT) and thermal withdrawal latency (TWL), resulting in a reduced sensitivity to pain stimuli. Moreover, both MET and MET-NE treatments promoted the\u0000 phosphorylation of AMP-activated protein kinase (AMPK) and acetyl-CoA carboxylase (ACC) proteins, as well as increased the activity of autophagic proteins in the sciatic nerve. However, no significant differences were observed between MET and MET-NE treatments in terms of these effects. In\u0000 conclusion, the MET-NE demonstrated a rapid decrease in blood glucose levels and improved glucose tolerance and metabolism, which was found to be superior to MET. Furthermore, MET-NE significantly improved the neurophysiological function and sciatic nerve pain threshold in DPN rats. These\u0000 beneficial effects may be attributed to the regulation of AMPK-mediated autophagy by MET-NE.","PeriodicalId":18318,"journal":{"name":"Materials Express","volume":" 2","pages":""},"PeriodicalIF":0.7,"publicationDate":"2023-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138615692","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"材料科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Xia Zhang, Lei Zhang, Rongbin Huang, Xianghui Zhang
Reinforced concrete beam bridges have been extensively utilized in transportation engineering due to their advantages such as simplified material selection and low cost. However, the mechanical performance of steel bars and concrete deteriorates over time due to factors such as prolonged bridge service life, fluctuations in environmental temperature and humidity, and other influences. To achieve a precise and effective prediction of the bearing capacity of reinforced concrete structures, this study proposes a method for assessing bearing capacity in the context of material performance degradation. The study commenced by examining the factors that impact the degradation of bridge performance and subsequently utilized material performance test values along with predicted and corrected values to estimate the reduced bearing capacity of the bridge. Additionally, the mechanical and bonding properties of modified reinforced concrete materials were calculated. The experiment results demonstrate that the calculated values from the modified model closely align with the measured values with a differential range of 0.06 to 0.38. At a micro level, nano-SiO2 modification enhances rubber concrete by improving the compactness of the matrix and enhancing bond strength between steel fiber-and-nanosilica-reinforced crumb rubber concrete and deformed steel bars. The revised model in this study exhibits excellent predictive capability for concrete strength, thereby enhancing the accuracy of outcomes in evaluating bridge technical conditions. This method holds potential for practical engineering applications and scientific research on bridge evaluation.
{"title":"Construction of a bearing capacity testing model for reinforced bridges based on material performance deterioration","authors":"Xia Zhang, Lei Zhang, Rongbin Huang, Xianghui Zhang","doi":"10.1166/mex.2023.2556","DOIUrl":"https://doi.org/10.1166/mex.2023.2556","url":null,"abstract":"Reinforced concrete beam bridges have been extensively utilized in transportation engineering due to their advantages such as simplified material selection and low cost. However, the mechanical performance of steel bars and concrete deteriorates over time due to factors such as prolonged\u0000 bridge service life, fluctuations in environmental temperature and humidity, and other influences. To achieve a precise and effective prediction of the bearing capacity of reinforced concrete structures, this study proposes a method for assessing bearing capacity in the context of material\u0000 performance degradation. The study commenced by examining the factors that impact the degradation of bridge performance and subsequently utilized material performance test values along with predicted and corrected values to estimate the reduced bearing capacity of the bridge. Additionally,\u0000 the mechanical and bonding properties of modified reinforced concrete materials were calculated. The experiment results demonstrate that the calculated values from the modified model closely align with the measured values with a differential range of 0.06 to 0.38. At a micro level, nano-SiO2\u0000 modification enhances rubber concrete by improving the compactness of the matrix and enhancing bond strength between steel fiber-and-nanosilica-reinforced crumb rubber concrete and deformed steel bars. The revised model in this study exhibits excellent predictive capability for concrete strength,\u0000 thereby enhancing the accuracy of outcomes in evaluating bridge technical conditions. This method holds potential for practical engineering applications and scientific research on bridge evaluation.","PeriodicalId":18318,"journal":{"name":"Materials Express","volume":" 34","pages":""},"PeriodicalIF":0.7,"publicationDate":"2023-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138616387","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"材料科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Currently, surgery is the only treatment that can completely remove gastric tumor. This study investigated the effect of curcumin on sevoflurane anesthesia-induced nerve damage in rats with gastric cancer (GC). A rat GC model was established and received sevoflurane for anesthesia. The rats were then divided into model group, low-dose (20 mg/kg), high-dose (60 mg/kg) group and blank control group (n = 5, each group). To identify the interaction between curcumin and mitogen-activated protein kinase (MAPK) expression, we set up MAPK mimic group, MAPK inhibitor group, and high-dose curcumin+MAPK inhibitor group (n = 5). The cognitive ability of rats after anesthesia and neuronal damage was assessed using Morris water maze test, whilst the expressions of brain-derived neurotrophic factor (BDNF), nerve growth factor (NGF) and pyroptosis were also detected. Administration of curcumin attenuated neuronal injury and pyroptosis in the hippocampus induced by sevoflurane, and facilitated the recovery of memory impairment in rats, resulting in decreased expression of MAPK and increased expressions of BNDF and NGF. After treatment with MAPK inhibitors, the nerve damage in the rat hippocampus tissue was controlled, and more prominently neuroprotective effect appeared in the curcumin high-dose+MAPK inhibitor group. Curcumin can thus effectively up-regulate BNDF and NGF in hippocampal tissue, which is closely related to inhibited MAPK pathway activity. Curcumin protects the nervous system by inhibiting the expression of pyroptin, contributing toimprovement of cognitive impairment in rats.
{"title":"Management of nerve injury by curcumin after sevoflurane anesthesia in gastric cancer through MAPK signaling pathway","authors":"Jianguang Ren, Ben Cai","doi":"10.1166/mex.2023.2545","DOIUrl":"https://doi.org/10.1166/mex.2023.2545","url":null,"abstract":"Currently, surgery is the only treatment that can completely remove gastric tumor. This study investigated the effect of curcumin on sevoflurane anesthesia-induced nerve damage in rats with gastric cancer (GC). A rat GC model was established and received sevoflurane for anesthesia.\u0000 The rats were then divided into model group, low-dose (20 mg/kg), high-dose (60 mg/kg) group and blank control group (n = 5, each group). To identify the interaction between curcumin and mitogen-activated protein kinase (MAPK) expression, we set up MAPK mimic group, MAPK inhibitor group,\u0000 and high-dose curcumin+MAPK inhibitor group (n = 5). The cognitive ability of rats after anesthesia and neuronal damage was assessed using Morris water maze test, whilst the expressions of brain-derived neurotrophic factor (BDNF), nerve growth factor (NGF) and pyroptosis were also detected.\u0000 Administration of curcumin attenuated neuronal injury and pyroptosis in the hippocampus induced by sevoflurane, and facilitated the recovery of memory impairment in rats, resulting in decreased expression of MAPK and increased expressions of BNDF and NGF. After treatment with MAPK inhibitors,\u0000 the nerve damage in the rat hippocampus tissue was controlled, and more prominently neuroprotective effect appeared in the curcumin high-dose+MAPK inhibitor group. Curcumin can thus effectively up-regulate BNDF and NGF in hippocampal tissue, which is closely related to inhibited MAPK pathway\u0000 activity. Curcumin protects the nervous system by inhibiting the expression of pyroptin, contributing toimprovement of cognitive impairment in rats.","PeriodicalId":18318,"journal":{"name":"Materials Express","volume":" 40","pages":""},"PeriodicalIF":0.7,"publicationDate":"2023-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138611707","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"材料科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Renal injury, which is a prevalent condition, significantly impacts the health of competitive wrestlers. However, there is a limited number of therapeutic methods and drugs available for its treatment. To tackle this issue, a renal targeted drug based on functionalized polymer nanoparticles (FPNPs) is being studied. The primary methods in the preparation of FPNPs involve utilizing the thin film hydration technique to create nanoparticles and encapsulating the drug TP within them as a treatment for renal injury, thus forming TP-FPNPs. Subsequently, the renal targeting of TP-FPNPs is analyzed using in vitro organ fluorescence imaging techniques. Furthermore, a renal IRI model is constructed to assess the pharmacokinetics of TP-FPNPs using the application method. The preparation results of TP-FPNPs reveal a hydrated particle size of approximately 90 nm, which remains in the renal region for over 48 hours. Additionally, the concentration content surpasses that of other organs by more than threefold after 12 hours. Moreover, in the application results, TP-FPNPs continue to possess a concentration of 302.5 ng/g even after 48 hours post-injection. Based on the aforementioned results, it can be concluded that TP-FPNPs exhibit specific targeting, biocompatibility, and an extended retention time. Thus, this study is expected to effectively achieve the effect of treating renal injury in martial arts athletes, establishing an experimental foundation for its clinical treatment.
{"title":"Preparation of functionalized polymer nanomaterials and application in renal injury","authors":"Qin Liu","doi":"10.1166/mex.2023.2554","DOIUrl":"https://doi.org/10.1166/mex.2023.2554","url":null,"abstract":"Renal injury, which is a prevalent condition, significantly impacts the health of competitive wrestlers. However, there is a limited number of therapeutic methods and drugs available for its treatment. To tackle this issue, a renal targeted drug based on functionalized polymer nanoparticles\u0000 (FPNPs) is being studied. The primary methods in the preparation of FPNPs involve utilizing the thin film hydration technique to create nanoparticles and encapsulating the drug TP within them as a treatment for renal injury, thus forming TP-FPNPs. Subsequently, the renal targeting of TP-FPNPs\u0000 is analyzed using in vitro organ fluorescence imaging techniques. Furthermore, a renal IRI model is constructed to assess the pharmacokinetics of TP-FPNPs using the application method. The preparation results of TP-FPNPs reveal a hydrated particle size of approximately 90 nm, which\u0000 remains in the renal region for over 48 hours. Additionally, the concentration content surpasses that of other organs by more than threefold after 12 hours. Moreover, in the application results, TP-FPNPs continue to possess a concentration of 302.5 ng/g even after 48 hours post-injection.\u0000 Based on the aforementioned results, it can be concluded that TP-FPNPs exhibit specific targeting, biocompatibility, and an extended retention time. Thus, this study is expected to effectively achieve the effect of treating renal injury in martial arts athletes, establishing an experimental\u0000 foundation for its clinical treatment.","PeriodicalId":18318,"journal":{"name":"Materials Express","volume":" 2","pages":""},"PeriodicalIF":0.7,"publicationDate":"2023-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138613458","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"材料科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Diabetic nephropathy (DN) is a common chronic disease affecting people’s life. Total glucosides of peony (TGP) regulates TLR4/NF-κB activation. This study aims at investigating the underlying mechanism of TGP in DN treatment. A retrospective analysis of clinical data was conducted to observe periodic acid-silver metheramine (PASM) staining and detect the expression of TLR4 and VEGF in DN. Additionally, rats were injected with Streptozocin (STZ) (30–60 mg) according to the ratio of 45–65 mg/kg to establish DN model. 8 weeks later, rats of experimental group were intraperitoneally injected with TGP. Kidney was collected for HE staining to observe morphological changes. Fibronectin expression was detected by histochemistry and the level of TLR4/NF-κB pathway-related proteins and apoptosis were detected by Western blot. PASM staining revealed focal tubular atrophy and interstitial fibrosis in DN patients. The renal medulla gradually disappeared and infiltration increased in control group, while inflammatory cells decreased in experimental group. The expression of fibronectin, TLR4 and p-p65 was decreased after TGP treatment. TGP treatment significantly decreased fibronectin expression and TGP treatment-induced inhibition of NF-κB signaling enhanced apoptotic activation of HK-2 cells, which was partially reversed by TLR4 and NF-κB inhibitors. TGP can inhibit inflammatory response of DN via regulating TLR4/NF-κB.
{"title":"Total glucosides of peony regulate toll-like receptor to inhibit inflammatory response in diabetic nephropathy","authors":"Lang Li, Huan Wang, Chunyu Zhao","doi":"10.1166/mex.2023.2568","DOIUrl":"https://doi.org/10.1166/mex.2023.2568","url":null,"abstract":"Diabetic nephropathy (DN) is a common chronic disease affecting people’s life. Total glucosides of peony (TGP) regulates TLR4/NF-κB activation. This study aims at investigating the underlying mechanism of TGP in DN treatment. A retrospective analysis of clinical data\u0000 was conducted to observe periodic acid-silver metheramine (PASM) staining and detect the expression of TLR4 and VEGF in DN. Additionally, rats were injected with Streptozocin (STZ) (30–60 mg) according to the ratio of 45–65 mg/kg to establish DN model. 8 weeks later, rats of experimental\u0000 group were intraperitoneally injected with TGP. Kidney was collected for HE staining to observe morphological changes. Fibronectin expression was detected by histochemistry and the level of TLR4/NF-κB pathway-related proteins and apoptosis were detected by Western blot. PASM staining\u0000 revealed focal tubular atrophy and interstitial fibrosis in DN patients. The renal medulla gradually disappeared and infiltration increased in control group, while inflammatory cells decreased in experimental group. The expression of fibronectin, TLR4 and p-p65 was decreased after TGP treatment.\u0000 TGP treatment significantly decreased fibronectin expression and TGP treatment-induced inhibition of NF-κB signaling enhanced apoptotic activation of HK-2 cells, which was partially reversed by TLR4 and NF-κB inhibitors. TGP can inhibit inflammatory response of DN\u0000 via regulating TLR4/NF-κB.","PeriodicalId":18318,"journal":{"name":"Materials Express","volume":" 24","pages":""},"PeriodicalIF":0.7,"publicationDate":"2023-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138611459","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"材料科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
京公网安备 11010802042870号
京ICP备2023020795号-1
扫码关注我们
求助内容：
应助结果提醒方式：