This study investigates the mechanism of montelukast intervention on autophagy and apoptosis of airway epithelial cells. Construction of HBE cell building model, which were intervened by montelukast. The proliferation of human 16HBE cells was detected using MTT method and β-catenin level was detected. The cellular cycle distribution, autophagy and apoptosis were detected using flow cytometry. And expressions of Transcriptional activator 3 (STAT3)-Retinoic acid-associated nuclear orphan receptor (RORγt)-interleukin 17 (IL-17)/interleukin 23 (IL-23) signaling related proteins were measured using Western blot. Montelukast inhibited the proliferation of human 16HBE cells and its inhibition rate and action concentration showed time and dose dependence. The half maximal inhibitory concentrations (IC50) were (12.8±0.67) μmol/L at 24 h, (8.8±0.43) μmol/L at 48 h and (6.6±0.42) μmol/L at 72 h, respectively. Montelukast induced 16HBE cellular cycle to arrest in G2/M phase dose-dependently (5, 10 and 20 μmol/L) (P <0.05) and simultaneously increased apoptosis rate (P < 0.05). 40 μL montelukast had a protective effect on 16HBE cells. In addition, montelukast reduced β-catenin level, which suggested that STAT3-RORγt-IL-17/IL-23 signaling pathway might be inhibited. Meanwhile, montelukast reduced the expressions of STAT3, P-STAT3, RORγt (RORβ), c-myc and survivin and increased protein expressions of GSK-3 (RORα) and Th17, but had no effect on the total RORγt level. Montelukast may effectively promote the apoptosis of 16HBE airway epithelial cells via inhibition of STAT3-RORγt-IL-17/IL-23 signaling.
本研究探讨了孟鲁司特干预气道上皮细胞自噬和凋亡的机制。构建经孟鲁司特干预的 HBE 细胞模型。用MTT法检测人16HBE细胞的增殖情况，并检测β-catenin水平。流式细胞术检测了细胞周期分布、自噬和凋亡。采用 Western 印迹法测定转录激活因子 3（STAT3）-Retinoic 酸相关核孤儿受体（RORγt）-白细胞介素 17（IL-17）/白细胞介素 23（IL-23）信号转导相关蛋白的表达。孟鲁司特能抑制人 16HBE 细胞的增殖，其抑制率和作用浓度与时间和剂量有关。半最大抑制浓度（IC50）分别为：24 h (12.8±0.67) μmol/L，48 h (8.8±0.43) μmol/L，72 h (6.6±0.42) μmol/L。孟鲁司特诱导 16HBE 细胞周期停滞在 G2/M 期的剂量依赖性（5、10 和 20 μmol/L）（P <0.05），并同时增加细胞凋亡率（P <0.05）。40 μL 孟鲁司特对 16HBE 细胞有保护作用。此外，孟鲁司特降低了β-catenin水平，这表明STAT3-RORγt-IL-17/IL-23信号通路可能受到了抑制。同时，孟鲁司特降低了STAT3、P-STAT3、RORγt（RORβ）、c-myc和survivin的表达，增加了GSK-3（RORα）和Th17的蛋白表达，但对总RORγt水平没有影响。孟鲁司特可通过抑制STAT3-RORγt-IL-17/IL-23信号传导，有效促进16HBE气道上皮细胞的凋亡。
{"title":"Mechanism of montelukast on autophagy and apoptosis of airway epithelial cells through STAT3-RORγt-IL-17/IL-23 signaling pathway","authors":"Hui Xu, Xiurong Wang, Bin Du","doi":"10.1166/mex.2024.2596","DOIUrl":"https://doi.org/10.1166/mex.2024.2596","url":null,"abstract":"This study investigates the mechanism of montelukast intervention on autophagy and apoptosis of airway epithelial cells. Construction of HBE cell building model, which were intervened by montelukast. The proliferation of human 16HBE cells was detected using MTT method and β-catenin level was detected. The cellular cycle distribution, autophagy and apoptosis were detected using flow cytometry. And expressions of Transcriptional activator 3 (STAT3)-Retinoic acid-associated nuclear orphan receptor (RORγt)-interleukin 17 (IL-17)/interleukin 23 (IL-23) signaling related proteins were measured using Western blot. Montelukast inhibited the proliferation of human 16HBE cells and its inhibition rate and action concentration showed time and dose dependence. The half maximal inhibitory concentrations (IC50) were (12.8±0.67) μmol/L at 24 h, (8.8±0.43) μmol/L at 48 h and (6.6±0.42) μmol/L at 72 h, respectively. Montelukast induced 16HBE cellular cycle to arrest in G2/M phase dose-dependently (5, 10 and 20 μmol/L) (P <0.05) and simultaneously increased apoptosis rate (P < 0.05). 40 μL montelukast had a protective effect on 16HBE cells. In addition, montelukast reduced β-catenin level, which suggested that STAT3-RORγt-IL-17/IL-23 signaling pathway might be inhibited. Meanwhile, montelukast reduced the expressions of STAT3, P-STAT3, RORγt (RORβ), c-myc and survivin and increased protein expressions of GSK-3 (RORα) and Th17, but had no effect on the total RORγt level. Montelukast may effectively promote the apoptosis of 16HBE airway epithelial cells via inhibition of STAT3-RORγt-IL-17/IL-23 signaling.","PeriodicalId":18318,"journal":{"name":"Materials Express","volume":"47 16","pages":""},"PeriodicalIF":0.7,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139126611","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"材料科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
In generator circuit breakers, monitoring the decomposition of Sulfur Hexafluoride (SF6) gas is a primary method to determine insulation and fault conditions. The presence of SF6 combined with H2O impurities also significantly impacts the degradation of equipment insulation. However, there is a lack of research on the simultaneous monitoring of both SF6 decomposition and H2O. To improve the system’s rapid recoverability, recyclability, and long-term usability, a SnSe monolayer doped with TiO2 nanoparticles (SnSe–TiO2) has been proposed as an SF6 gas decomposer detection sensor with humidity detection capabilities. The SnSe–TiO2 monolayer significantly enhances the conductivity and increases its adsorption energy for H2O (73.2%), H2S (13.54%), HF (59.70%), SO2 (96.33%), and SOF2 (52.04%). Furthermore, this material can be utilized for long-term cyclic gas monitoring as the SnSe–TiO2 monolayer is physically adsorbed to these gases and can be rapidly desorbed. This study establishes a theoretical foundation for the future advancement of gas detection sensors for insulating gases in circuit breakers.
{"title":"Long-term monitoring of SF6 decomposition gases and water molecules using TiO2-doped SnSe monolayer for generator circuit breakers","authors":"Rufei He, Li Cheng, Xiaofeng Huang, Hao Xu, Xiaojing Zhang, Xiaobo Zhang","doi":"10.1166/mex.2024.2590","DOIUrl":"https://doi.org/10.1166/mex.2024.2590","url":null,"abstract":"In generator circuit breakers, monitoring the decomposition of Sulfur Hexafluoride (SF6) gas is a primary method to determine insulation and fault conditions. The presence of SF6 combined with H2O impurities also significantly impacts the degradation of equipment insulation. However, there is a lack of research on the simultaneous monitoring of both SF6 decomposition and H2O. To improve the system’s rapid recoverability, recyclability, and long-term usability, a SnSe monolayer doped with TiO2 nanoparticles (SnSe–TiO2) has been proposed as an SF6 gas decomposer detection sensor with humidity detection capabilities. The SnSe–TiO2 monolayer significantly enhances the conductivity and increases its adsorption energy for H2O (73.2%), H2S (13.54%), HF (59.70%), SO2 (96.33%), and SOF2 (52.04%). Furthermore, this material can be utilized for long-term cyclic gas monitoring as the SnSe–TiO2 monolayer is physically adsorbed to these gases and can be rapidly desorbed. This study establishes a theoretical foundation for the future advancement of gas detection sensors for insulating gases in circuit breakers.","PeriodicalId":18318,"journal":{"name":"Materials Express","volume":"59 49","pages":""},"PeriodicalIF":0.7,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139126673","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"材料科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
The design of switched-mode power supplies (SMPSs) is a material-electrical engineering interdisciplinary problem. The design of magnetic component materials can significantly affect SMPSs’ performances. In engineering education, the design and development of solutions is an important skill for engineering students. However, the traditional engineering experiments curriculum is shown as validation and single-disciplinary experiments as well as one-sided assessment results. To foster open-ended exploration, enhance students’ material design skills, and improve skills adaptation, students must tackle real engineering problems and develop practical design solutions. This paper proposes an exploratory factor analysis and knowledge graph-based method for evaluating and continuously improving magnetic components material design skills in the context of SMPS design tasks. First, we used the multiple imputation method to address the missing data. Each imputed data was analyzed to extract factors through parallel analysis, ordinary least squares estimation, and target rotation. Then, we identified four sub-skills: efficiency design skill, passive device design skill, power magnetic reduction design skill, and power economy design skill. The RMSEA for the four-factor model is 0.042, suggesting a good fit to the data. We established the relationships between these sub-skills and SMPS performance metrics. Furthermore, the average of the factor scores from each of the imputed datasets and the SMPS design constraints were combined to obtain the cut-off scores to evaluate engineering students’ achievements in these sub-skills. Finally, we constructed an SMPS magnetic components material knowledge graph, which could recommend specific experimental tasks, relevant knowledge areas, and SMPS performance metrics, providing personalized guidance to designers.
{"title":"Evaluation and continuous improvement of magnetic components material design skills in interdisciplinary switched-mode power supply","authors":"Yi Kuang, Zhiyong Zhang, Bin Duan","doi":"10.1166/mex.2024.2581","DOIUrl":"https://doi.org/10.1166/mex.2024.2581","url":null,"abstract":"The design of switched-mode power supplies (SMPSs) is a material-electrical engineering interdisciplinary problem. The design of magnetic component materials can significantly affect SMPSs’ performances. In engineering education, the design and development of solutions is an important skill for engineering students. However, the traditional engineering experiments curriculum is shown as validation and single-disciplinary experiments as well as one-sided assessment results. To foster open-ended exploration, enhance students’ material design skills, and improve skills adaptation, students must tackle real engineering problems and develop practical design solutions. This paper proposes an exploratory factor analysis and knowledge graph-based method for evaluating and continuously improving magnetic components material design skills in the context of SMPS design tasks. First, we used the multiple imputation method to address the missing data. Each imputed data was analyzed to extract factors through parallel analysis, ordinary least squares estimation, and target rotation. Then, we identified four sub-skills: efficiency design skill, passive device design skill, power magnetic reduction design skill, and power economy design skill. The RMSEA for the four-factor model is 0.042, suggesting a good fit to the data. We established the relationships between these sub-skills and SMPS performance metrics. Furthermore, the average of the factor scores from each of the imputed datasets and the SMPS design constraints were combined to obtain the cut-off scores to evaluate engineering students’ achievements in these sub-skills. Finally, we constructed an SMPS magnetic components material knowledge graph, which could recommend specific experimental tasks, relevant knowledge areas, and SMPS performance metrics, providing personalized guidance to designers.","PeriodicalId":18318,"journal":{"name":"Materials Express","volume":"21 13","pages":""},"PeriodicalIF":0.7,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139126342","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"材料科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Yang Zhang, Mengying Xiong, Shibiao Chen, Ningyan Wang, Xiuhong Wang
Perioperative neurocognitive disorders (PND) are postoperative or anesthesia-related complications of the central nervous system, characterized by intelligence decline, memory loss, attention deficit, impaired decision-making skills, reduced language comprehension performance, and psychological disorders. This study aims to investigate the impact of a subanesthetic dose of esketamine on PND in aged mice by regulating the microglia toll-like receptor 4 (TLR4)/nuclear factor κB (NF-κB) pathway. An exploratory laparotomy model was established in 18-month-old C57BL/6 mice post-anesthesia. Behavioral evaluations such as open field tests and Morris water maze tests for autonomous activity and spatial learning and memory ability. Different esketamine doses were administered to aged mice, followed by behavioral assessment for autonomous activity and learning/memory abilities. Nucleic acid extraction employed magnetic nanoparticles, while qRT-PCR measured mRNA levels of tumor necrosis factor-α, interleukin-6, and interleukin-1β. Western Blot detected the Iba-1 and TLR4 protein expression and phosphorylation status of the P65 protein. Immunofluorescence determined the fluorescence intensity of Iba-1 and P-NF-κBp65. MTT assay evaluated cell viability. As a result, we demonstrated that a subanesthetic dose of esketamine effectively inhibited microglia activation and TLR4/NF-κB signaling pathway both in vivo and in vitro, resulting in decreased levels of inflammatory factors and improved neuroinflammatory responses, ultimately alleviating postoperative neurocognitive dysfunction. Notably, our findings revealed that laparotomy exploratory surgery-induced cognitive dysfunction, which can be improved by esketamine’s neuroprotective effects. Furthermore, our study also highlighted the protective effect of esketamine against lipopolysaccharide-induced inflammatory response in BV2 cells by inhibiting the TLR4/NF-κB signaling pathway.
{"title":"Effect and mechanism of a subanesthetic dose of esketamine on postoperative neurocognitive disorders through the regulating of the TLR4/NF-κB pathway in microglia","authors":"Yang Zhang, Mengying Xiong, Shibiao Chen, Ningyan Wang, Xiuhong Wang","doi":"10.1166/mex.2024.2583","DOIUrl":"https://doi.org/10.1166/mex.2024.2583","url":null,"abstract":"Perioperative neurocognitive disorders (PND) are postoperative or anesthesia-related complications of the central nervous system, characterized by intelligence decline, memory loss, attention deficit, impaired decision-making skills, reduced language comprehension performance, and psychological disorders. This study aims to investigate the impact of a subanesthetic dose of esketamine on PND in aged mice by regulating the microglia toll-like receptor 4 (TLR4)/nuclear factor κB (NF-κB) pathway. An exploratory laparotomy model was established in 18-month-old C57BL/6 mice post-anesthesia. Behavioral evaluations such as open field tests and Morris water maze tests for autonomous activity and spatial learning and memory ability. Different esketamine doses were administered to aged mice, followed by behavioral assessment for autonomous activity and learning/memory abilities. Nucleic acid extraction employed magnetic nanoparticles, while qRT-PCR measured mRNA levels of tumor necrosis factor-α, interleukin-6, and interleukin-1β. Western Blot detected the Iba-1 and TLR4 protein expression and phosphorylation status of the P65 protein. Immunofluorescence determined the fluorescence intensity of Iba-1 and P-NF-κBp65. MTT assay evaluated cell viability. As a result, we demonstrated that a subanesthetic dose of esketamine effectively inhibited microglia activation and TLR4/NF-κB signaling pathway both in vivo and in vitro, resulting in decreased levels of inflammatory factors and improved neuroinflammatory responses, ultimately alleviating postoperative neurocognitive dysfunction. Notably, our findings revealed that laparotomy exploratory surgery-induced cognitive dysfunction, which can be improved by esketamine’s neuroprotective effects. Furthermore, our study also highlighted the protective effect of esketamine against lipopolysaccharide-induced inflammatory response in BV2 cells by inhibiting the TLR4/NF-κB signaling pathway.","PeriodicalId":18318,"journal":{"name":"Materials Express","volume":"81 6","pages":""},"PeriodicalIF":0.7,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139127601","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"材料科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Nanoscale drug delivery systems and biomedical sensors are witnessing huge research interests. Polymeric nanoparticles, nanofibers, liposomes, and nano-emulsion are regarded as novel sensors and drug delivery systems including the various types of wound dressings formulated by micro/nanofibers, hydrogels, hydrocolloids, films, foams, and sponges. Consequently, the electro-spun nanofibrous mats received the most attention since the drugs and nanoparticles embedded nanofibers have been recognized as the potential candidates for sensors and wound dressing applications. These composite materials have superior surface area-to-volume ratio, high nano-porosity, distinct skin extracellular matrix structure, and ease of electro-spinning, which also supports a prolonged drug release. In this study the main advantages of the nano-fibrillary network and the active substances that can be incorporated in fabricating the composite nanofibers as sensors and wound dressing materials are discussed. The promising “Pluronic F68” and polyvinyl alcohol (PVA), materials are used including nanoparticles (NPs) of titanium dioxide (TiO2), silver, and cobalt. The difficulties that arise by converting the Pluronic into nanofibers via electrospinning due to its low melting point and beads formation and the blending of this material with polyvinyl alcohol (PVA) are discussed. The electro-spinability of the pure Pluronic, PVA and their blends are evaluated. The microstructures and morphologies of the fabricated composite nanofibers and structures are characterized using scanning electron microscopy (SEM), thermogravimetric analysis (TGA), differential thermal analysis (DSC), Fourier transform infrared (FTIR) spectroscopy, and transmission electron microscopy (TEM). The fabricated blended nanofibers were found to be compact and entangled having a diameter from 50 nm to 80 nm and length ranging from 1 to 3 μm. Meanwhile, the pure Pluronic produced nano-beads with an average size of 40 nm. These nanocomposites can be used in biomedical applications such as biosensors by embedding nanoparticles of TiO2, silver, and cobalt and for antibacterial treatment in woundcare dressings.
{"title":"Characterization of electrospun pluronic F68/polyvinyl alcohol nanofibers composites containing titanium dioxide, silver, and cobalt nanoparticles for biomedical applications","authors":"Mahir Es-Saheb, Yasser Fouad, Khalid A. Ibrahim","doi":"10.1166/mex.2024.2609","DOIUrl":"https://doi.org/10.1166/mex.2024.2609","url":null,"abstract":"Nanoscale drug delivery systems and biomedical sensors are witnessing huge research interests. Polymeric nanoparticles, nanofibers, liposomes, and nano-emulsion are regarded as novel sensors and drug delivery systems including the various types of wound dressings formulated by micro/nanofibers, hydrogels, hydrocolloids, films, foams, and sponges. Consequently, the electro-spun nanofibrous mats received the most attention since the drugs and nanoparticles embedded nanofibers have been recognized as the potential candidates for sensors and wound dressing applications. These composite materials have superior surface area-to-volume ratio, high nano-porosity, distinct skin extracellular matrix structure, and ease of electro-spinning, which also supports a prolonged drug release. In this study the main advantages of the nano-fibrillary network and the active substances that can be incorporated in fabricating the composite nanofibers as sensors and wound dressing materials are discussed. The promising “Pluronic F68” and polyvinyl alcohol (PVA), materials are used including nanoparticles (NPs) of titanium dioxide (TiO2), silver, and cobalt. The difficulties that arise by converting the Pluronic into nanofibers via electrospinning due to its low melting point and beads formation and the blending of this material with polyvinyl alcohol (PVA) are discussed. The electro-spinability of the pure Pluronic, PVA and their blends are evaluated. The microstructures and morphologies of the fabricated composite nanofibers and structures are characterized using scanning electron microscopy (SEM), thermogravimetric analysis (TGA), differential thermal analysis (DSC), Fourier transform infrared (FTIR) spectroscopy, and transmission electron microscopy (TEM). The fabricated blended nanofibers were found to be compact and entangled having a diameter from 50 nm to 80 nm and length ranging from 1 to 3 μm. Meanwhile, the pure Pluronic produced nano-beads with an average size of 40 nm. These nanocomposites can be used in biomedical applications such as biosensors by embedding nanoparticles of TiO2, silver, and cobalt and for antibacterial treatment in woundcare dressings.","PeriodicalId":18318,"journal":{"name":"Materials Express","volume":"65 8","pages":""},"PeriodicalIF":0.7,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139128828","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"材料科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Shoujian Zong, Xiaojie Hu, Shouwei Zong, Guizhi Sun
Acute ischemic stroke (AIS) is featured as damage of blood-brain barrier in severe cases, which leads to brain tissue damage. Panax notoginseng saponins (PNS), because of its anti-inflammatory effect, can reduce the inflammatory response caused by ischemia-reperfusion, thereby reducing the degree of damage to the blood-brain barrier. Studies on AIS have confirmed that, Cazenne has an anti-inflammatory effect, but specific mechanism by which PNS regulates Cazenne to reduce the inflammatory response after cerebral ischemia-reperfusion injury (IRI) is still unclear. Therefore, this study explored PNS’ role in inhibiting the inflammatory response after cerebral IRI through Cezanne. Sprague-Dawley (SD) rats were randomly grouped and then assigned into sham operation group (sham), model group (MCAO), model+PNS (MCAO+PNS), model+PNS+Cezanne silencing lentivirus (MCAO+PNS+shCezanne), and model+PNS+empty carrier (MCAO+PNS+shNC). Neurological function was scored by mNSS while cerebral infarction volume was tested by TCC staining. The brain was tested by dry and wet method, while levels of inflammatory factors and NLRP1 and Caspase-1 were detected by ELISA, Western blot, and Cazenne expression was detected by immunofluorescence. PNS reduced the expression of pro-inflammatory factors in MCAO rats, by mainly downregulating NLRP-1 and Caspase-1. By upregulating Cezanne and inflammatory factors downstream NLRP1/Caspase-1 pathway was inhibited, thereby improving the inflammation in the rats after IRI. PNS inhibited the activity of NLRP1/Caspase-1 signaling pathway through Cezanne, thereby reducing inflammatory factors in the brain after cerebral IRI, and reducing inflammatory response.
{"title":"Reduction of inflammatory response after ischemia-reperfusion injury in rats with panax notoginseng saponins by regulating nucleotide-bound oligomerized domain-like receptor protein 1(NLRP1)/Caspase-1 signaling via Cezanne","authors":"Shoujian Zong, Xiaojie Hu, Shouwei Zong, Guizhi Sun","doi":"10.1166/mex.2024.2595","DOIUrl":"https://doi.org/10.1166/mex.2024.2595","url":null,"abstract":"Acute ischemic stroke (AIS) is featured as damage of blood-brain barrier in severe cases, which leads to brain tissue damage. Panax notoginseng saponins (PNS), because of its anti-inflammatory effect, can reduce the inflammatory response caused by ischemia-reperfusion, thereby reducing the degree of damage to the blood-brain barrier. Studies on AIS have confirmed that, Cazenne has an anti-inflammatory effect, but specific mechanism by which PNS regulates Cazenne to reduce the inflammatory response after cerebral ischemia-reperfusion injury (IRI) is still unclear. Therefore, this study explored PNS’ role in inhibiting the inflammatory response after cerebral IRI through Cezanne. Sprague-Dawley (SD) rats were randomly grouped and then assigned into sham operation group (sham), model group (MCAO), model+PNS (MCAO+PNS), model+PNS+Cezanne silencing lentivirus (MCAO+PNS+shCezanne), and model+PNS+empty carrier (MCAO+PNS+shNC). Neurological function was scored by mNSS while cerebral infarction volume was tested by TCC staining. The brain was tested by dry and wet method, while levels of inflammatory factors and NLRP1 and Caspase-1 were detected by ELISA, Western blot, and Cazenne expression was detected by immunofluorescence. PNS reduced the expression of pro-inflammatory factors in MCAO rats, by mainly downregulating NLRP-1 and Caspase-1. By upregulating Cezanne and inflammatory factors downstream NLRP1/Caspase-1 pathway was inhibited, thereby improving the inflammation in the rats after IRI. PNS inhibited the activity of NLRP1/Caspase-1 signaling pathway through Cezanne, thereby reducing inflammatory factors in the brain after cerebral IRI, and reducing inflammatory response.","PeriodicalId":18318,"journal":{"name":"Materials Express","volume":"16 12","pages":""},"PeriodicalIF":0.7,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139127889","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"材料科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Shiying Guo, Hong Chen, Ying Xu, Doudou Ding, Aihua Chen
Intrauterine adhesion (IUA) is a common complication in endometrial disorder. This study investigated the role of ATI-2341, a functionally selective allosteric regulator of CXCR4 in IUAs, and its interaction with bone marrow-derived mesenchymal stem cells (BMSCs). Following establishment of endometrial injury model, rats BMSCs were treated with ATI-2341 TFA (100 ng/mL). Endometrial tissues of rats with IUA were treated with BMSCs and estrogen, or untreated which was taken as controls. The endometrium thickness was examined and endometrial BMSC proliferation was detected by MTT assay. Levels of MMP-9, TIMP-1, CK, and VIM were determined by Western blot analysis. Treatment with ATI-2341 TFA resulted in significantly decreased level of MMP-9 (0.346±0.036 ng/mL), compared with estrogen treatment (0.467±0.029 ng/mL) and control treatment. Both BMSCs carrying ATI-2341 TFA and estrogen induced increased expression of TIMP-1 (0.734±0.034 ng/mL, 0.618±0.035 ng/mL) and enhanced endometrim growth with thicker endometrium in ATI-2341 TFA group (294.21±59.97 μm). 48 h and 72 h after treatment, ATI-2341 TFA group and estrogen group exhibited increased proliferation rate (P <0.05), and higher rate appeared upon ATI-2341 TFA treatment. Cells in ATI-2341 TFA100 ng/mL and estrogen group were greatly positive for keratin and negative for vimentin. Collectively, ATI-2341 TFA promotes the differentiation of BMSCs into endometrial epithelial cells, enhances repair of damaged endometrium, and alleviate IUA. These findings might underlie a foundation for BMSC-based treatment for endometrial disorder.
{"title":"ATI-2341 TFA promotes repair of damaged endometrium by mediating the differentiation of bone marrow mesenchymal stem cells","authors":"Shiying Guo, Hong Chen, Ying Xu, Doudou Ding, Aihua Chen","doi":"10.1166/mex.2024.2576","DOIUrl":"https://doi.org/10.1166/mex.2024.2576","url":null,"abstract":"Intrauterine adhesion (IUA) is a common complication in endometrial disorder. This study investigated the role of ATI-2341, a functionally selective allosteric regulator of CXCR4 in IUAs, and its interaction with bone marrow-derived mesenchymal stem cells (BMSCs). Following establishment of endometrial injury model, rats BMSCs were treated with ATI-2341 TFA (100 ng/mL). Endometrial tissues of rats with IUA were treated with BMSCs and estrogen, or untreated which was taken as controls. The endometrium thickness was examined and endometrial BMSC proliferation was detected by MTT assay. Levels of MMP-9, TIMP-1, CK, and VIM were determined by Western blot analysis. Treatment with ATI-2341 TFA resulted in significantly decreased level of MMP-9 (0.346±0.036 ng/mL), compared with estrogen treatment (0.467±0.029 ng/mL) and control treatment. Both BMSCs carrying ATI-2341 TFA and estrogen induced increased expression of TIMP-1 (0.734±0.034 ng/mL, 0.618±0.035 ng/mL) and enhanced endometrim growth with thicker endometrium in ATI-2341 TFA group (294.21±59.97 μm). 48 h and 72 h after treatment, ATI-2341 TFA group and estrogen group exhibited increased proliferation rate (P <0.05), and higher rate appeared upon ATI-2341 TFA treatment. Cells in ATI-2341 TFA100 ng/mL and estrogen group were greatly positive for keratin and negative for vimentin. Collectively, ATI-2341 TFA promotes the differentiation of BMSCs into endometrial epithelial cells, enhances repair of damaged endometrium, and alleviate IUA. These findings might underlie a foundation for BMSC-based treatment for endometrial disorder.","PeriodicalId":18318,"journal":{"name":"Materials Express","volume":"3 4","pages":""},"PeriodicalIF":0.7,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139124974","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"材料科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Coronary atherosclerotic heart failure (CAHF) is a common clinical cardiovascular disease that seriously endangers human health. Combination of recombinant human brain natriuretic peptide and sacubitril-valsartan sodium tablets (rhBNP-SV) may have a positive effect on CAHF patients. However, there have been few research on this. In this study, 120 patients with CAHF were collected, 30 of them were used as control group and 90 patients were randomly divided into sacubitril-valsartan sodium tablet group, rhBNP group and combination group. The cardiac function-related indicators and serum mitochondrial coupling factor 6, ACE and others were examined. The levels of various cardiac function indexes in CAHF patients showed an upward trend and the increase was the most obvious in combination group. Patients in combination group had the longest walking distance within 6 minutes. Levels of the four groups of patients showed a downward trend and the intervention of rhBNP-VS showed the most significant decrease. Proportion of IFN-γ-positive lymphocytes in the total T lymphocytes gradually decreased with different interventions. Under rhBNP-SV intervention, ACE level in patients was significantly inhibited. The treatment of rhBNP-SV improved the exercise capacity of patients, reduced inflammatory response, and regulated ACE expression in patients with CAHF. Furthermore, it reduced the degree of vascular endothelial injury, inhibited the development of the disease, and ultimately enhanced the therapeutic effect to a large extent.
{"title":"Effect of recombinant human brain natriuretic peptide combined with sacubitril and valsartan sodium tablets on the condition of patients with coronary atherosclerotic heart failure","authors":"Jun Fan, Chunxiao Yan","doi":"10.1166/mex.2024.2589","DOIUrl":"https://doi.org/10.1166/mex.2024.2589","url":null,"abstract":"Coronary atherosclerotic heart failure (CAHF) is a common clinical cardiovascular disease that seriously endangers human health. Combination of recombinant human brain natriuretic peptide and sacubitril-valsartan sodium tablets (rhBNP-SV) may have a positive effect on CAHF patients. However, there have been few research on this. In this study, 120 patients with CAHF were collected, 30 of them were used as control group and 90 patients were randomly divided into sacubitril-valsartan sodium tablet group, rhBNP group and combination group. The cardiac function-related indicators and serum mitochondrial coupling factor 6, ACE and others were examined. The levels of various cardiac function indexes in CAHF patients showed an upward trend and the increase was the most obvious in combination group. Patients in combination group had the longest walking distance within 6 minutes. Levels of the four groups of patients showed a downward trend and the intervention of rhBNP-VS showed the most significant decrease. Proportion of IFN-γ-positive lymphocytes in the total T lymphocytes gradually decreased with different interventions. Under rhBNP-SV intervention, ACE level in patients was significantly inhibited. The treatment of rhBNP-SV improved the exercise capacity of patients, reduced inflammatory response, and regulated ACE expression in patients with CAHF. Furthermore, it reduced the degree of vascular endothelial injury, inhibited the development of the disease, and ultimately enhanced the therapeutic effect to a large extent.","PeriodicalId":18318,"journal":{"name":"Materials Express","volume":"58 3","pages":""},"PeriodicalIF":0.7,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139125192","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"材料科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Ytterbium modified TiO2 nanoparticles (Yb@TiO2), synthesized by a sol–gel process, were employed as an efficient adsorbent and matrix for the analysis of Perfluorooctane sulfonate (PFOS) and Tetrabromobisphenol A (TBBPA) via surface-enhanced laser desorption/ionization time-of-flight mass spectrometry (SELDI-TOF MS). The detection limits for PFOS and TBBPA are achievement with 0.01 pg ·mL−1. The results of the analysis of camellia oil samples show good recovery (80.3%–86.8%) with a low detection limit for TBBPA.
采用溶胶-凝胶法合成的镱修饰二氧化钛纳米粒子（Yb@TiO2）被用作一种高效吸附剂和基质，通过表面增强激光解吸电离飞行时间质谱（SELDI-TOF MS）分析全氟辛烷磺酸（PFOS）和四溴双酚A（TBBPA）。全氟辛烷磺酸和四溴双酚 A 的检测限均为 0.01 pg -mL-1。山茶油样品的分析结果表明，回收率较高（80.3%-86.8%），TBBPA 的检出限较低。
{"title":"Yb@TiO2 nanoparticles as a SELDI-TOF MS matrix for the simultaneous measurement of Tetrabromobisphenol A and Perfluorooctane sulfonate","authors":"Jing Yang, Chunhua Luo, Lihao Xiong, Lichun Fu, Xiaohu Luo, Linxiao Gao, Huang Zhou, Lixia Yang, Fei Yang, Deshuai Zhen","doi":"10.1166/mex.2024.2591","DOIUrl":"https://doi.org/10.1166/mex.2024.2591","url":null,"abstract":"Ytterbium modified TiO2 nanoparticles (Yb@TiO2), synthesized by a sol–gel process, were employed as an efficient adsorbent and matrix for the analysis of Perfluorooctane sulfonate (PFOS) and Tetrabromobisphenol A (TBBPA) via surface-enhanced laser desorption/ionization time-of-flight mass spectrometry (SELDI-TOF MS). The detection limits for PFOS and TBBPA are achievement with 0.01 pg ·mL−1. The results of the analysis of camellia oil samples show good recovery (80.3%–86.8%) with a low detection limit for TBBPA.","PeriodicalId":18318,"journal":{"name":"Materials Express","volume":"26 12","pages":""},"PeriodicalIF":0.7,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139129054","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"材料科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Jiansheng Yang, Di Wu, Liling Zeng, Minzhi Zhong, Tianshan Liang, Honghong Jiang, Qiang Liu, Yanhua Wu, Hui Li
This study analyzes the differences in potential biomarkers of migraine models constructed by two commonly used modeling methods through metabolomics research. Migraine models were constructed by nitroglycerin (NTG) induction or mechanical stimulus-induced cortical spreading depression (CSD) in rats. Multivariate analysis was used for metabolomic analysis. Compared with control group, NTG migraine group had 1 different metabolite up-regulated and 14 down-regulated. Compared with NTG migraine group, 13 differential metabolites in the CSD migraine model group were up-regulated and 12 were down-regulated. Both nitroglycerin-induced and mechanical stimulation-induced CSD can construct migraine rat models, and the biomarkers of the two models are different.
{"title":"Metabonomics of brain tissue in migraine rat model induced by nitroglycerin or cortical spreading depression","authors":"Jiansheng Yang, Di Wu, Liling Zeng, Minzhi Zhong, Tianshan Liang, Honghong Jiang, Qiang Liu, Yanhua Wu, Hui Li","doi":"10.1166/mex.2024.2579","DOIUrl":"https://doi.org/10.1166/mex.2024.2579","url":null,"abstract":"This study analyzes the differences in potential biomarkers of migraine models constructed by two commonly used modeling methods through metabolomics research. Migraine models were constructed by nitroglycerin (NTG) induction or mechanical stimulus-induced cortical spreading depression (CSD) in rats. Multivariate analysis was used for metabolomic analysis. Compared with control group, NTG migraine group had 1 different metabolite up-regulated and 14 down-regulated. Compared with NTG migraine group, 13 differential metabolites in the CSD migraine model group were up-regulated and 12 were down-regulated. Both nitroglycerin-induced and mechanical stimulation-induced CSD can construct migraine rat models, and the biomarkers of the two models are different.","PeriodicalId":18318,"journal":{"name":"Materials Express","volume":"112 7","pages":""},"PeriodicalIF":0.7,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139126074","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"材料科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
京公网安备 11010802042870号
京ICP备2023020795号-1
扫码关注我们
求助内容：
应助结果提醒方式：