Pub Date : 2019-01-01DOI: 10.22037/AMLS.V5I3.30773
A. Niroomand
Background: Nowadays the use of herbs as an alternative to the chemical drug is considered by researchers. Aloe vera belongs to the Asphodelaceae family, a medicinal plant that has been used since ancient times for different pharmaceutical products. This study aimed to evaluate the antibacterial properties of Aloe vera grown in Khouzestan, southwest of Iran. Materials and Methods:For this purpose, ethanol extract was prepared from aerial parts of Aloe vera and its activity was tested against some gram-positive (Enterococcus faecalis, Staphylococcus aureus, Bacillus cereus) and gram-negative (Pseudomonass aeruginosa, Salmonella Typhi, Proteus mirabilis) bacterial species through standard Kirby-Bauer disc diffusion method. Minimum inhibitory concentration and minimum bactericidal concentration were also investigated. Results: The Aloe vera extract showed antibacterial activity against the majority of bacteria. The highest activity (about 25mm inhibition zone) happened against P. aeruginosa but it did not show any inhibitory activity against S.aureus and P. mirabilis. The MIC was found as 10 mg/ml while MBC ≥ 80. Conclusion: Based on the results of this study it can be suggested that Aloe vera contains active antibacterial substances that can be used efficiently for bacterial pathogen control and it should be considered as a potent antimicrobial source for finding new antibiotics, especially against resistant species.
{"title":"Assessment of the antibacterial potential of Aloe vera as a source of antibacterial agents","authors":"A. Niroomand","doi":"10.22037/AMLS.V5I3.30773","DOIUrl":"https://doi.org/10.22037/AMLS.V5I3.30773","url":null,"abstract":"Background: Nowadays the use of herbs as an alternative to the chemical drug is considered by researchers. Aloe vera belongs to the Asphodelaceae family, a medicinal plant that has been used since ancient times for different pharmaceutical products. This study aimed to evaluate the antibacterial properties of Aloe vera grown in Khouzestan, southwest of Iran. Materials and Methods:For this purpose, ethanol extract was prepared from aerial parts of Aloe vera and its activity was tested against some gram-positive (Enterococcus faecalis, Staphylococcus aureus, Bacillus cereus) and gram-negative (Pseudomonass aeruginosa, Salmonella Typhi, Proteus mirabilis) bacterial species through standard Kirby-Bauer disc diffusion method. Minimum inhibitory concentration and minimum bactericidal concentration were also investigated. Results: The Aloe vera extract showed antibacterial activity against the majority of bacteria. The highest activity (about 25mm inhibition zone) happened against P. aeruginosa but it did not show any inhibitory activity against S.aureus and P. mirabilis. The MIC was found as 10 mg/ml while MBC ≥ 80. Conclusion: Based on the results of this study it can be suggested that Aloe vera contains active antibacterial substances that can be used efficiently for bacterial pathogen control and it should be considered as a potent antimicrobial source for finding new antibiotics, especially against resistant species.","PeriodicalId":18401,"journal":{"name":"Medical laboratory sciences","volume":"4 1","pages":"21-25"},"PeriodicalIF":0.0,"publicationDate":"2019-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"88230943","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2019-01-01DOI: 10.22037/AMLS.V5I2.29313
Z. Babaei, Nikou Bahrami, S. Khalili, Iman Pouladi
Backgrounds: Cholera is an acute diarrheal intestinal infection caused by infection of intestine with vibrio cholera. Cholera is defined as endemic in Iran, thus rapid detection in the early stage of infection is crucial, especially during the disease outbreaks. Materials and Methods: We collected 172 rectal swab samples from patients with diarrheal infections, and sterile beakers were used to collect water samples from some regions of Pol-e Dokhtar city. The collected specimens were transported to the laboratory using Cary-Blair transport medium and were then grown and incubated on alkaline peptone water (APW) and thiosulfate citrate bile salts sucrose (TCBS) agar. Then, all colonies that grew in Kligler Iron Agar (KIA) were cultured. Results: In this study, colonies were grown on APW and TCBS media, and were then transferred to the KIA. None of the colonies were shown to be belonged to V. cholerae strains. Thus, all collected samples were negative for V. cholerae in all patients as well as the drinking water in most regions of the city. Conclusion: The findings of this study suggest that V. cholerae was not the major cause of severe diarrheal infections in a flood-hit population of Pol-e Dokhtar city, and therefore other bacterial and viral agents should be responsible for severe diarrheal illnesses in this population.
{"title":"The prevalence of Vibrio Cholera Infection among Patients with Diarrheal Infections in flood affected population of Pol-e Dokhtar County: 2019","authors":"Z. Babaei, Nikou Bahrami, S. Khalili, Iman Pouladi","doi":"10.22037/AMLS.V5I2.29313","DOIUrl":"https://doi.org/10.22037/AMLS.V5I2.29313","url":null,"abstract":"Backgrounds: Cholera is an acute diarrheal intestinal infection caused by infection of intestine with vibrio cholera. Cholera is defined as endemic in Iran, thus rapid detection in the early stage of infection is crucial, especially during the disease outbreaks. Materials and Methods: We collected 172 rectal swab samples from patients with diarrheal infections, and sterile beakers were used to collect water samples from some regions of Pol-e Dokhtar city. The collected specimens were transported to the laboratory using Cary-Blair transport medium and were then grown and incubated on alkaline peptone water (APW) and thiosulfate citrate bile salts sucrose (TCBS) agar. Then, all colonies that grew in Kligler Iron Agar (KIA) were cultured. Results: In this study, colonies were grown on APW and TCBS media, and were then transferred to the KIA. None of the colonies were shown to be belonged to V. cholerae strains. Thus, all collected samples were negative for V. cholerae in all patients as well as the drinking water in most regions of the city. Conclusion: The findings of this study suggest that V. cholerae was not the major cause of severe diarrheal infections in a flood-hit population of Pol-e Dokhtar city, and therefore other bacterial and viral agents should be responsible for severe diarrheal illnesses in this population.","PeriodicalId":18401,"journal":{"name":"Medical laboratory sciences","volume":"80 1","pages":"1-3"},"PeriodicalIF":0.0,"publicationDate":"2019-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"87131689","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2019-01-01DOI: 10.22037/AMLS.V5I2.29771
pedram Attaredelgosha
Background: The use of herbal remedies, either in combination with conventional drugs or as an alternative, is accepted worldwide. Silymarin derived from Milk thistle has evidence-based therapeutic potency for wide spectrum of liver diseases. The current work aimed to study the immune-modulating activity of Silymarin in HCV-infected patients by measuring the effect of pure Silymarin solution on the production of IL-17 and IL-10. Materials and Methods: Nine HCV-1a infected patients and three healthy controls were entered in this study. The mean age of patients and healthy controls were 45.53 (± 10.21) and 39.9 (±10.88), respectively. The PBMCs were isolated, cultured in 96-well plate and incubated with Silymarin solution (5µg/ml) for 24 hours. The cells and cell culture supernatant of three groups including patients treated with Silymarin, non-treated patients, and healthy controls were then subjected to Real Time PCR and ELISA to measure the levels of inflammatory and non-inflammatory cytokines including IL-17 and IL-10, respectively. Statistical analysis was conducted using SPSS software version 20.0. Results: According to Real Time PCR and ELISA results, the level of IL-17 was significantly reduced in patients treated with Silymarin while the expression of IL-10 was remarkably increased in these patients. Conclusion: The results of this study approved the immunomodulatory properties of Silymarin in HCV-infected patients. Hepatoprotective, antiviral, as well as immunomodulatory properties of the Silymarin make it a potential therapeutic option in patients with chronic hepatitis C.
背景:草药疗法的使用，无论是与常规药物联合使用还是作为一种替代疗法，在世界范围内都是被接受的。从水飞蓟中提取的水飞蓟素对广泛的肝脏疾病具有循证的治疗效力。本研究旨在通过测定水飞蓟素纯溶液对hcv感染患者IL-17和IL-10产生的影响，研究水飞蓟素对hcv感染患者的免疫调节活性。材料与方法:选取9例HCV-1a感染患者和3例健康对照。患者和健康对照组的平均年龄分别为45.53(±10.21)岁和39.9(±10.88)岁。分离PBMCs，在96孔板中培养，用水飞蓟素溶液(5µg/ml)孵育24小时。将水飞蓟素治疗组、未治疗组和健康对照组的细胞和细胞培养上清分别采用Real - Time PCR和ELISA检测炎症和非炎症细胞因子IL-17和IL-10的水平。采用SPSS 20.0软件进行统计分析。结果:Real Time PCR和ELISA结果显示，水飞蓟素治疗组IL-17水平明显降低，IL-10表达明显升高。结论:水飞蓟素对hcv感染患者具有免疫调节作用。水飞蓟素的肝保护、抗病毒和免疫调节特性使其成为慢性丙型肝炎患者的潜在治疗选择。
{"title":"Evaluation of the Sylimarine Effect on IL-10 and IL-17 Production in Blood Samples of Chronic HCV Infected patients","authors":"pedram Attaredelgosha","doi":"10.22037/AMLS.V5I2.29771","DOIUrl":"https://doi.org/10.22037/AMLS.V5I2.29771","url":null,"abstract":"Background: The use of herbal remedies, either in combination with conventional drugs or as an alternative, is accepted worldwide. Silymarin derived from Milk thistle has evidence-based therapeutic potency for wide spectrum of liver diseases. The current work aimed to study the immune-modulating activity of Silymarin in HCV-infected patients by measuring the effect of pure Silymarin solution on the production of IL-17 and IL-10. Materials and Methods: Nine HCV-1a infected patients and three healthy controls were entered in this study. The mean age of patients and healthy controls were 45.53 (± 10.21) and 39.9 (±10.88), respectively. The PBMCs were isolated, cultured in 96-well plate and incubated with Silymarin solution (5µg/ml) for 24 hours. The cells and cell culture supernatant of three groups including patients treated with Silymarin, non-treated patients, and healthy controls were then subjected to Real Time PCR and ELISA to measure the levels of inflammatory and non-inflammatory cytokines including IL-17 and IL-10, respectively. Statistical analysis was conducted using SPSS software version 20.0. Results: According to Real Time PCR and ELISA results, the level of IL-17 was significantly reduced in patients treated with Silymarin while the expression of IL-10 was remarkably increased in these patients. Conclusion: The results of this study approved the immunomodulatory properties of Silymarin in HCV-infected patients. Hepatoprotective, antiviral, as well as immunomodulatory properties of the Silymarin make it a potential therapeutic option in patients with chronic hepatitis C.","PeriodicalId":18401,"journal":{"name":"Medical laboratory sciences","volume":"21 1","pages":"10-14"},"PeriodicalIF":0.0,"publicationDate":"2019-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"85278450","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2019-01-01DOI: 10.22037/AMLS.V5I1.30140
M. Tavana, N. Varasteh, Mohadeseh Rohban, H. Ghazizadeh, M. Sadeghzade, S. Tavalaie, Samaneh Abolbashari, H. Ramshini, Z. Meshkat, G. Ferns, M. Mobarhan, F. Sadabadi
Background: Diabetes mellitus is an important risk factor for cardiovascular disease. Different biomarkers have been investigated for the diagnosis of diabetes pathogenesis or its complications. There are also reports regarding an increased level of anti-HSP27 antibodies in atherogenesis. We aimed to evaluate serum anti-heat shock protein 27 antibodies level in subjects with diabetes mellitus and undiagnosed individuals. Materials and Methods: This cross-sectional study was conducted on 6447 MASHAD study subjects, including four groups with diabetes mellitus (n=610), undiagnosed diabetes (n=162), impaired fasting glucose (IFG) (n=619) and normal (n=5056) subjects. Demographic and anthropometric data were obtained from all participants. Fasting serum glucose (FSG) and other parameters were measured. In-house enzyme-linked immune sorbent assay method was used for measuring Anti-HSP27 antibodies levels. Results: There were significant differences in weight (p=0.034), body mass index, waist, and hip circumference, systolic and diastolic blood pressure, fasting serum glucose, lipid profile and high sensitive- C reactive protein (p<0.001) between four groups of diabetes mellitus, undiagnosed diabetes, impaired fasting glucose, and normal subjects. The serum anti-HSP27 antibody titer did not show a significant difference between studied groups. Conclusion: Serum antibody titers to HSP27 were not significantly different between four groups categorized based on their FSG levels in a large population.
{"title":"Serum anti-hsp27 antibodies concentration in diabetes mellitus; population based case-control study","authors":"M. Tavana, N. Varasteh, Mohadeseh Rohban, H. Ghazizadeh, M. Sadeghzade, S. Tavalaie, Samaneh Abolbashari, H. Ramshini, Z. Meshkat, G. Ferns, M. Mobarhan, F. Sadabadi","doi":"10.22037/AMLS.V5I1.30140","DOIUrl":"https://doi.org/10.22037/AMLS.V5I1.30140","url":null,"abstract":"Background: Diabetes mellitus is an important risk factor for cardiovascular disease. Different biomarkers have been investigated for the diagnosis of diabetes pathogenesis or its complications. There are also reports regarding an increased level of anti-HSP27 antibodies in atherogenesis. We aimed to evaluate serum anti-heat shock protein 27 antibodies level in subjects with diabetes mellitus and undiagnosed individuals. Materials and Methods: This cross-sectional study was conducted on 6447 MASHAD study subjects, including four groups with diabetes mellitus (n=610), undiagnosed diabetes (n=162), impaired fasting glucose (IFG) (n=619) and normal (n=5056) subjects. Demographic and anthropometric data were obtained from all participants. Fasting serum glucose (FSG) and other parameters were measured. In-house enzyme-linked immune sorbent assay method was used for measuring Anti-HSP27 antibodies levels. Results: There were significant differences in weight (p=0.034), body mass index, waist, and hip circumference, systolic and diastolic blood pressure, fasting serum glucose, lipid profile and high sensitive- C reactive protein (p<0.001) between four groups of diabetes mellitus, undiagnosed diabetes, impaired fasting glucose, and normal subjects. The serum anti-HSP27 antibody titer did not show a significant difference between studied groups. Conclusion: Serum antibody titers to HSP27 were not significantly different between four groups categorized based on their FSG levels in a large population.","PeriodicalId":18401,"journal":{"name":"Medical laboratory sciences","volume":"60 1","pages":"12-17"},"PeriodicalIF":0.0,"publicationDate":"2019-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"90153402","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2019-01-01DOI: 10.22037/AMLS.V5I2.30110
Sakineh Monzavi, M. Shahhosseiny, Mahsa Malekmohammadi Kalahroudi
Background: Congenital infections are the most important threat to human fetal health caused by bacterial, viral, and fungal agents. In this study, PCR diagnostic method has been used on umbilical cord samples to diagnose bacterial agents such as Mycoplasmas, especially Mycoplasma genitalium. Materials and Methods: This study was performed on 100 cord blood samples from cesarean section patients. Limit of detection (LOD) and specificity tests of both PCR was performed. DNA samples were extracted by the DNG plus method and amplified by the PCR technique. The optimized PCR product of 715 bp for Mycoplasma spp. and 427 bp for M. genitalium was amplified and observed on 1.5% gel electrophoresis. DNA specificity testing of seven other organisms revealed a 100% specificity of these primers. The detection limit was set at 100 copy / reaction for Mycoplasma.spp and 1000 copy / reaction for M. genitalium. Results: Of the 100 samples examined, 5 samples were positive for Mycoplasma spp, and no positive cases were observed for M. genitalium. Maternal infections can have important consequences on the fetus. Conclusion: Molecular methods such as PCR could be used for rapid identification of important factors in congenital infections, such as mycoplasma, and provide a good prognosis.
{"title":"Evaluation of PCR for the Identification of Mycoplasma spp. and Mycoplasma genitalium congenital infection in cord blood","authors":"Sakineh Monzavi, M. Shahhosseiny, Mahsa Malekmohammadi Kalahroudi","doi":"10.22037/AMLS.V5I2.30110","DOIUrl":"https://doi.org/10.22037/AMLS.V5I2.30110","url":null,"abstract":"Background: Congenital infections are the most important threat to human fetal health caused by bacterial, viral, and fungal agents. In this study, PCR diagnostic method has been used on umbilical cord samples to diagnose bacterial agents such as Mycoplasmas, especially Mycoplasma genitalium. Materials and Methods: This study was performed on 100 cord blood samples from cesarean section patients. Limit of detection (LOD) and specificity tests of both PCR was performed. DNA samples were extracted by the DNG plus method and amplified by the PCR technique. The optimized PCR product of 715 bp for Mycoplasma spp. and 427 bp for M. genitalium was amplified and observed on 1.5% gel electrophoresis. DNA specificity testing of seven other organisms revealed a 100% specificity of these primers. The detection limit was set at 100 copy / reaction for Mycoplasma.spp and 1000 copy / reaction for M. genitalium. Results: Of the 100 samples examined, 5 samples were positive for Mycoplasma spp, and no positive cases were observed for M. genitalium. Maternal infections can have important consequences on the fetus. Conclusion: Molecular methods such as PCR could be used for rapid identification of important factors in congenital infections, such as mycoplasma, and provide a good prognosis.","PeriodicalId":18401,"journal":{"name":"Medical laboratory sciences","volume":"56 1","pages":"29-35"},"PeriodicalIF":0.0,"publicationDate":"2019-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"74790965","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2019-01-01DOI: 10.22037/AMLS.V5I3.31303
E. Rostami, Zakieh Sadat Sheikhalishahi, Vahideh Behmard, Mohammad Panji, Fatemeh Sadat Moravej, A. Namdari, Sina Dalvand, Azita Aliakbarniya, Negar Yavari, Vajiheh Najafi
Background: Vitamin D deficiency and insufficiency generally observed in chronic kidney disease (CKD) and dialysis patients. The goal of this work was to compare the serum levels of vitamin D and its-related metabolic parameters in CKD patients and hemodialysis patients. Materials and Methods: A total of 109 dialysis patients and 99 CKD patients who had not consumed vitamin D for ≥ 1 year were enrolled in this study. 25-Hydroxy vitamin D (25-(OH) D) levels, calcium, alkaline phosphatase (ALP), hemoglobin (Hb), hematocrit (HCT), total iron-binding capacity (TIBC), parathyroid hormone (PTH) and phosphorous were evaluated in the serum of the patients. Results: 25-(OH) D levels in patients with CKD and dialysis patients were 31.73±13.34 ng/mL and 15.52±5.36 ng/mL, respectively. The levels of calcium (P=0.000), ALP (P=0.006), 25(OH) D (P=0.000), FBS (P=0.000), Hb (P=0.002), HCT (P=0.002) and TIBC (P=0.000) were significantly higher, and the levels of PTH (p=0.000) and phosphorous (P=0.000) were significantly lower in the CKD group compared to dialysis group. Conclusion: 25-(OH) D levels and its metabolic parameters except PTH and phosphorus are lower in patients on dialysis compared with the patients with CKD.
{"title":"A Comparative Study of 25-Hydroxyvitamin D Level and associated Parameters in CKD and Dialysis Patients in Shiraz, Iran","authors":"E. Rostami, Zakieh Sadat Sheikhalishahi, Vahideh Behmard, Mohammad Panji, Fatemeh Sadat Moravej, A. Namdari, Sina Dalvand, Azita Aliakbarniya, Negar Yavari, Vajiheh Najafi","doi":"10.22037/AMLS.V5I3.31303","DOIUrl":"https://doi.org/10.22037/AMLS.V5I3.31303","url":null,"abstract":"Background: Vitamin D deficiency and insufficiency generally observed in chronic kidney disease (CKD) and dialysis patients. The goal of this work was to compare the serum levels of vitamin D and its-related metabolic parameters in CKD patients and hemodialysis patients. Materials and Methods: A total of 109 dialysis patients and 99 CKD patients who had not consumed vitamin D for ≥ 1 year were enrolled in this study. 25-Hydroxy vitamin D (25-(OH) D) levels, calcium, alkaline phosphatase (ALP), hemoglobin (Hb), hematocrit (HCT), total iron-binding capacity (TIBC), parathyroid hormone (PTH) and phosphorous were evaluated in the serum of the patients. Results: 25-(OH) D levels in patients with CKD and dialysis patients were 31.73±13.34 ng/mL and 15.52±5.36 ng/mL, respectively. The levels of calcium (P=0.000), ALP (P=0.006), 25(OH) D (P=0.000), FBS (P=0.000), Hb (P=0.002), HCT (P=0.002) and TIBC (P=0.000) were significantly higher, and the levels of PTH (p=0.000) and phosphorous (P=0.000) were significantly lower in the CKD group compared to dialysis group. Conclusion: 25-(OH) D levels and its metabolic parameters except PTH and phosphorus are lower in patients on dialysis compared with the patients with CKD.","PeriodicalId":18401,"journal":{"name":"Medical laboratory sciences","volume":"2 1","pages":"16-20"},"PeriodicalIF":0.0,"publicationDate":"2019-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"73257587","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2019-01-01DOI: 10.22037/AMLS.V5I1.28475
kosar eskandari
Earlier detection of diseases reduces the mortality rate. So the development of better screening techniques could be considered as a main topic of interest. In non-invasive medical imaging, specifically in Magnetic Resonance Imaging (MRI), conventional contrast agents do not have a good performance in imaging of some fine parts. The prosperity of preclinical researches in oncology that purpose at developing and evaluating curative strategies on samples, requires effective new functionalized contrast agents for portrayal tumor growth, monitoring the trace of a treatment and/or inducing the demolition of cancerous tumors. This study was performed by searching Nanotechnology, Molecular imaging, magnetic nanoparticles, and contrast agent nanoparticles keywords in Google scholar, Science direct, PubMed and Scopus websites in terms of content. We reviewed the recent studies about development of nanoparticles as contrast agents for medical imaging because they have a longer vascular half-life than molecular contrast agents. It could be indicate that nanoparticles are important items in increasing the contrast of the images so that even reducing the size of the magnetic nanoparticles escalates the contrast and half-life of the particles. Particles with a diameter of 10 nm have a greater half-life than particles with a diameter of 30 nm or larger. It was also found that to removing material defects or improve their biocompatibility; particles should be covered with other materials or doped with metals.
{"title":"Magnetic nanoparticles to improve the contrast of Magnetic Resonance Imaging","authors":"kosar eskandari","doi":"10.22037/AMLS.V5I1.28475","DOIUrl":"https://doi.org/10.22037/AMLS.V5I1.28475","url":null,"abstract":"Earlier detection of diseases reduces the mortality rate. So the development of better screening techniques could be considered as a main topic of interest. In non-invasive medical imaging, specifically in Magnetic Resonance Imaging (MRI), conventional contrast agents do not have a good performance in imaging of some fine parts. The prosperity of preclinical researches in oncology that purpose at developing and evaluating curative strategies on samples, requires effective new functionalized contrast agents for portrayal tumor growth, monitoring the trace of a treatment and/or inducing the demolition of cancerous tumors. This study was performed by searching Nanotechnology, Molecular imaging, magnetic nanoparticles, and contrast agent nanoparticles keywords in Google scholar, Science direct, PubMed and Scopus websites in terms of content. We reviewed the recent studies about development of nanoparticles as contrast agents for medical imaging because they have a longer vascular half-life than molecular contrast agents. It could be indicate that nanoparticles are important items in increasing the contrast of the images so that even reducing the size of the magnetic nanoparticles escalates the contrast and half-life of the particles. Particles with a diameter of 10 nm have a greater half-life than particles with a diameter of 30 nm or larger. It was also found that to removing material defects or improve their biocompatibility; particles should be covered with other materials or doped with metals.","PeriodicalId":18401,"journal":{"name":"Medical laboratory sciences","volume":"41 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2019-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"88548488","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2019-01-01DOI: 10.22037/AMLS.V5I1.27828
M. Eizadi, Z. Mirakhori, Ashraf Amini
Background: The insulin receptor substrate-1 (IRS-1) has an important role in insulin signaling pathways in the target tissue of obese or insulin-resistant individuals. This study aimed to assess the effect of resistance training on fasting glucose, insulin resistance, and IRS-1 gene expression in gastrocnemius muscle in male Wister rats with type 2 diabetes (T2D). Materials and Methods: For this purpose, T2D induced by high- fat diet (8 weeks) and STZ in fourteen male Wistar rats (220 ± 10 g) and then assigned into exercise (resistance training, 8 weeks, 5 days/weekly, n = 7) and control (no-training, n = 7) by randomly. Fasting blood samples were obtained for measuring glucose, insulin, and calculating insulin resistance (HOMA-IR). Also, the IRS-1 gene expression in gastrocnemius muscle was measured 48 hours after the last training session of both cases and controls. Results: Compared to control, IRS-1 gene expression in gastrocnemius muscle increased significantly by resistance training in exercise groups (p = 0.001). Fasting glucose (p < 0.001) and insulin resistance (p = 0.007) were reduced in the exercise rats compared to the control group. Conclusion: Based on the results, improved fasting glucose and insulin function after resistance training in T2D diabetes could be attributed to enhancing IRS-1 expression in gastrocnemius muscle by training.
{"title":"The effect of 8-week resistance training on IRS-1 gene expression in gastrocnemius muscle and glycemic profile in diabetes rats","authors":"M. Eizadi, Z. Mirakhori, Ashraf Amini","doi":"10.22037/AMLS.V5I1.27828","DOIUrl":"https://doi.org/10.22037/AMLS.V5I1.27828","url":null,"abstract":"Background: The insulin receptor substrate-1 (IRS-1) has an important role in insulin signaling pathways in the target tissue of obese or insulin-resistant individuals. This study aimed to assess the effect of resistance training on fasting glucose, insulin resistance, and IRS-1 gene expression in gastrocnemius muscle in male Wister rats with type 2 diabetes (T2D). Materials and Methods: For this purpose, T2D induced by high- fat diet (8 weeks) and STZ in fourteen male Wistar rats (220 ± 10 g) and then assigned into exercise (resistance training, 8 weeks, 5 days/weekly, n = 7) and control (no-training, n = 7) by randomly. Fasting blood samples were obtained for measuring glucose, insulin, and calculating insulin resistance (HOMA-IR). Also, the IRS-1 gene expression in gastrocnemius muscle was measured 48 hours after the last training session of both cases and controls. Results: Compared to control, IRS-1 gene expression in gastrocnemius muscle increased significantly by resistance training in exercise groups (p = 0.001). Fasting glucose (p < 0.001) and insulin resistance (p = 0.007) were reduced in the exercise rats compared to the control group. Conclusion: Based on the results, improved fasting glucose and insulin function after resistance training in T2D diabetes could be attributed to enhancing IRS-1 expression in gastrocnemius muscle by training.","PeriodicalId":18401,"journal":{"name":"Medical laboratory sciences","volume":"522 1","pages":"23-30"},"PeriodicalIF":0.0,"publicationDate":"2019-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"88962492","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2019-01-01DOI: 10.22037/AMLS.V5I2.31180
Fatemeh Ekhtesari, Lida Moghadam-Banaem, S. Shahbazi, S. Shahali
Background: To evaluate the birth weight and post-natal non-coagulation problems of infants of mothers with coagulopathies versus normal mothers. Materials and Methods: In a retrospective cohort study, 100 women with coagulation problems attended the Iranian Haemophilia Comprehensive Care Center, and 200 normal women attending two health centers in Tehran and Karaj, with a history of at least one pregnancy were studied. A questionnaire about mothers’ and neonates’ condition was filled out by an interview, and the data were analyzed using SPSS software, version 16. Results: Using linear regression, the maternal coagulation problem had a negative effect on birth weight (p<0.001, β= -0.31). The prevalence of early and prolonged jaundice in newborns of mothers with coagulopathy was higher than that in newborns of normal mothers (12% vs. 2%, and 7% vs. 3%, respectively), the difference between the two groups in terms of incidence of early jaundice was statistically significant (chi square-p<0.001). The frequency of using phototherapy and blood exchange for treating neonatal jaundice, in neonates of mothers with coagulopathy was higher than in neonates of normal mothers (31% vs. 21% and 8% vs. 21%, respectively) (p<0.001). Furthermore, the duration of hospitalization, hospitalization in NICU, and re-hospitalization during the first month of life in neonates of mothers with coagulation problems was higher than in neonates of normal mothers (p<0.001). Conclusion: Considering the high prevalence of neonatal problems in newborns of mothers with coagulation problems and their lower birth weight, further care measures should be provided for them.
{"title":"Effects of Maternal Coagulation disorder on Birth Weight and Post-Natal Non-Coagulation Problems of Neonates","authors":"Fatemeh Ekhtesari, Lida Moghadam-Banaem, S. Shahbazi, S. Shahali","doi":"10.22037/AMLS.V5I2.31180","DOIUrl":"https://doi.org/10.22037/AMLS.V5I2.31180","url":null,"abstract":"Background: To evaluate the birth weight and post-natal non-coagulation problems of infants of mothers with coagulopathies versus normal mothers. Materials and Methods: In a retrospective cohort study, 100 women with coagulation problems attended the Iranian Haemophilia Comprehensive Care Center, and 200 normal women attending two health centers in Tehran and Karaj, with a history of at least one pregnancy were studied. A questionnaire about mothers’ and neonates’ condition was filled out by an interview, and the data were analyzed using SPSS software, version 16. Results: Using linear regression, the maternal coagulation problem had a negative effect on birth weight (p<0.001, β= -0.31). The prevalence of early and prolonged jaundice in newborns of mothers with coagulopathy was higher than that in newborns of normal mothers (12% vs. 2%, and 7% vs. 3%, respectively), the difference between the two groups in terms of incidence of early jaundice was statistically significant (chi square-p<0.001). The frequency of using phototherapy and blood exchange for treating neonatal jaundice, in neonates of mothers with coagulopathy was higher than in neonates of normal mothers (31% vs. 21% and 8% vs. 21%, respectively) (p<0.001). Furthermore, the duration of hospitalization, hospitalization in NICU, and re-hospitalization during the first month of life in neonates of mothers with coagulation problems was higher than in neonates of normal mothers (p<0.001). Conclusion: Considering the high prevalence of neonatal problems in newborns of mothers with coagulation problems and their lower birth weight, further care measures should be provided for them.","PeriodicalId":18401,"journal":{"name":"Medical laboratory sciences","volume":"1 1","pages":"36-44"},"PeriodicalIF":0.0,"publicationDate":"2019-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"83612701","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2018-10-31DOI: 10.22037/AMLS.V3I1.17869
Maryam Bidram, F. Behzadian, F. Fotouhi, M. Fazeli
Background: The influenza virus hemagglutinin is the major surface protein of the influenza A virus which is composed of HA1 and HA2 subunits. HA1 has an important role in binding of virus to cells and designing neutralizing antibodies. Escherichia coli ( E. coli ) and Bacillus subtilis ( B. subtilis ) both are known as the most useful prokaryotic hosts to express recombinant proteins. The aim of this study was to clone and express recombinant HA1protein in E. coli and B. subtilis bacteria. Materials and Methods: HA1 gene was cloned into pET-28a vector and pHT43 shuttle vector and then, both transformed to E. coli . The recombinant plasmids were extracted and then transformed into the BL21 and WB600 as expressing hosts. After induction with isopropyl-β-d-thiogalactoside (IPTG), expressed recombinant protein was analyzed by SDS-PAGE. Finally, the expressed protein was confirmed by the Western blot. Results: HA1 gene was cloned into pET-28a vector and pHT43 shuttle vector and then, both transformed to E. coli .The recombinant plasmids were extracted and then transformed into the BL21 and WB600 as expressing hosts. After induction with isopropyl-β-d-thiogalactoside (IPTG), expressed recombinant protein was analyzed by SDS-PAGE. Finally, the expressed protein was confirmed by the Western blot. Conclusion: This study demonstrated a strategy for production and purification of recombinant protein in large scale to test as vaccine candidate against influenza and it’s potentially immunogenicity be assessed in animal models.
{"title":"Cloning and prokaryotic expression of the globular head domain of hemagglutinin antigen (HA1) of influenza A (H3N2) virus in Escherichia coli and Bacillus subtilis","authors":"Maryam Bidram, F. Behzadian, F. Fotouhi, M. Fazeli","doi":"10.22037/AMLS.V3I1.17869","DOIUrl":"https://doi.org/10.22037/AMLS.V3I1.17869","url":null,"abstract":"Background: The influenza virus hemagglutinin is the major surface protein of the influenza A virus which is composed of HA1 and HA2 subunits. HA1 has an important role in binding of virus to cells and designing neutralizing antibodies. Escherichia coli ( E. coli ) and Bacillus subtilis ( B. subtilis ) both are known as the most useful prokaryotic hosts to express recombinant proteins. The aim of this study was to clone and express recombinant HA1protein in E. coli and B. subtilis bacteria. Materials and Methods: HA1 gene was cloned into pET-28a vector and pHT43 shuttle vector and then, both transformed to E. coli . The recombinant plasmids were extracted and then transformed into the BL21 and WB600 as expressing hosts. After induction with isopropyl-β-d-thiogalactoside (IPTG), expressed recombinant protein was analyzed by SDS-PAGE. Finally, the expressed protein was confirmed by the Western blot. Results: HA1 gene was cloned into pET-28a vector and pHT43 shuttle vector and then, both transformed to E. coli .The recombinant plasmids were extracted and then transformed into the BL21 and WB600 as expressing hosts. After induction with isopropyl-β-d-thiogalactoside (IPTG), expressed recombinant protein was analyzed by SDS-PAGE. Finally, the expressed protein was confirmed by the Western blot. Conclusion: This study demonstrated a strategy for production and purification of recombinant protein in large scale to test as vaccine candidate against influenza and it’s potentially immunogenicity be assessed in animal models.","PeriodicalId":18401,"journal":{"name":"Medical laboratory sciences","volume":"27 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2018-10-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"86907853","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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