E. Dehghan, A. Ravasi, R. Nouri, A. Gaeini, M. Nekooei, M. Delavari, Masoud Khorsandi Kolur
Background. Aerobic training somewhat corrects endothelial dysfunction in patients with coronary artery disease (CAD), but the most effective form of exercise is still unclear; therefore, the purpose of the present study was to investigate the effect of 8 weeks of continuous aerobic exercise on serum vascular endothelial growth factor (VEGF) as an angiogenic factor and endostatin (ES) as an angiostatic factor in male rats with coronary artery disease. Methods. The subjects of the present experimental study were 30 healthy male 2-months old Wistar rats divided into three groups: the experimental group, the control group with coronary artery disease, and the healthy control group. After rupturing 20 of the rats by isoproterenol, 10 rats in the experimental group performed eight weeks of continuous aerobic exercise on a treadmill, with an incremental intensity of 50% in the first session to 78% in the last session, whereas both control groups did not exercise. Seventy-two hours after the final training session, blood samples were taken for serum VEGF and ES indices, and one-way ANOVA with Scheffer posthoc tests was used to compare the mean of variables among the studied groups. Results. The results showed that discontinuous aerobic exercise could increase the blood VEGF in rats with coronary artery disease (P≤0.01), while there was no difference in the level of endostatin in the experimental group compared with control groups (P>0.05). Conclusion. It seems that continuous aerobic exercises can be used in the rehabilitation of patients with coronary artery disease and might be effective in the process of angiogenesis.
{"title":"The effect of continuous aerobic exercise on serum angiogenic and angiostatic factors in induction model of acute myocardial infarction male rats with coronary artery disease","authors":"E. Dehghan, A. Ravasi, R. Nouri, A. Gaeini, M. Nekooei, M. Delavari, Masoud Khorsandi Kolur","doi":"10.34172/mj.2021.070","DOIUrl":"https://doi.org/10.34172/mj.2021.070","url":null,"abstract":"Background. Aerobic training somewhat corrects endothelial dysfunction in patients with coronary artery disease (CAD), but the most effective form of exercise is still unclear; therefore, the purpose of the present study was to investigate the effect of 8 weeks of continuous aerobic exercise on serum vascular endothelial growth factor (VEGF) as an angiogenic factor and endostatin (ES) as an angiostatic factor in male rats with coronary artery disease. Methods. The subjects of the present experimental study were 30 healthy male 2-months old Wistar rats divided into three groups: the experimental group, the control group with coronary artery disease, and the healthy control group. After rupturing 20 of the rats by isoproterenol, 10 rats in the experimental group performed eight weeks of continuous aerobic exercise on a treadmill, with an incremental intensity of 50% in the first session to 78% in the last session, whereas both control groups did not exercise. Seventy-two hours after the final training session, blood samples were taken for serum VEGF and ES indices, and one-way ANOVA with Scheffer posthoc tests was used to compare the mean of variables among the studied groups. Results. The results showed that discontinuous aerobic exercise could increase the blood VEGF in rats with coronary artery disease (P≤0.01), while there was no difference in the level of endostatin in the experimental group compared with control groups (P>0.05). Conclusion. It seems that continuous aerobic exercises can be used in the rehabilitation of patients with coronary artery disease and might be effective in the process of angiogenesis.","PeriodicalId":18474,"journal":{"name":"Medical journal of Tabriz University of Medical Sciences and Health Services","volume":"97 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"81270611","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
L. Emrahi, Mehrnoush Toufan Tabrizi, H. Tajsharghi
Objective: Hypertrophic cardiomyopathy is a most common genetic cardiovascular disease which is predominantly inherited with autosomal dominant pattern. In this study, Genetic analysis of a family affected by Hypertrophic cardiomyopathy with three patients was done using whole exome sequencing. Materials and Methods: whole exome sequencing was performed on two affected individuals and one healthy family member. Candidate variant was evaluated using sanger sequencing on other family members. Given the novelity of candidate variant, it was also studied in 200 healthy individuals. Different bioinformatics analyzes were performed to evaluate variant pathogenicity. Results: The candidate variant was present in two affected patients as well as in a two apparently healthy sibling. This variant was novel and did not exist in any databases. Echocardiographic results on family members showed that another proband’s brother was affected and didn’t have a candidate variant. Also, this variant was not found in a 200 healthy population. Bioinformatic analysis revealed the pathogenicity of the variant. Conclusion: Although all the early studies of the candidate variant showed it as a causative variant , further studies on more individuals of the family rejected its causality alone and from these results it can be concluded that the results of exome sequencing should be analyzed carefully and it need further study in a pedigrees with more affected individuals and functional studies to prove the variant as a pathogenic.
{"title":"New mutation in the NEBL gene in a family with Hypertrophic cardiomyopathy: Complexity of whole exome sequencing results interpretation","authors":"L. Emrahi, Mehrnoush Toufan Tabrizi, H. Tajsharghi","doi":"10.34172/mj.2021.059","DOIUrl":"https://doi.org/10.34172/mj.2021.059","url":null,"abstract":"Objective: Hypertrophic cardiomyopathy is a most common genetic cardiovascular disease which is predominantly inherited with autosomal dominant pattern. In this study, Genetic analysis of a family affected by Hypertrophic cardiomyopathy with three patients was done using whole exome sequencing. Materials and Methods: whole exome sequencing was performed on two affected individuals and one healthy family member. Candidate variant was evaluated using sanger sequencing on other family members. Given the novelity of candidate variant, it was also studied in 200 healthy individuals. Different bioinformatics analyzes were performed to evaluate variant pathogenicity. Results: The candidate variant was present in two affected patients as well as in a two apparently healthy sibling. This variant was novel and did not exist in any databases. Echocardiographic results on family members showed that another proband’s brother was affected and didn’t have a candidate variant. Also, this variant was not found in a 200 healthy population. Bioinformatic analysis revealed the pathogenicity of the variant. Conclusion: Although all the early studies of the candidate variant showed it as a causative variant , further studies on more individuals of the family rejected its causality alone and from these results it can be concluded that the results of exome sequencing should be analyzed carefully and it need further study in a pedigrees with more affected individuals and functional studies to prove the variant as a pathogenic.","PeriodicalId":18474,"journal":{"name":"Medical journal of Tabriz University of Medical Sciences and Health Services","volume":"56 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-09-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"80025016","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
S. Sane, Rahman Abasi vash, Farzaneh Joshaghani, Gonash Alizadeh, B. Gholamveisi, Parang Golabi
Backgrounds and objectives: Acute postoperative pain is still an issue in patients undergoing lumbar laminectomy surgery. We compared the effects of remifentanil and dexmedetomidine on pain intensity as well as the analgesic requirements in patients undergoing spinal surgery. Methods: This randomized clinical trial study was conducted on 60 patients in the age range of 20 to 60 years with ASA class one and two of the American Anesthesia Association under elective lumbar vertebrae laminectomy. Patients were divided into two groups: Remifentanil (group R) and Dexmedetomidine (group D). Before induction of anesthesia in R group patients, 0.01 می g / kg / min remifentanil was used. In group D, 0.3 dexmedetomidine was injected from µg / min / 50kg using a syringe pump. Postoperative pain score, nausea, and vomiting, mean arterial blood pressure, and heart rate at recovery times, 6, 12, and 24 hours after surgery were recorded. Results: The propofol infusion rate was significantly lower in the DEX group than in group R (P = 0.001). Pain scores except for 6h after surgery was significantly lower in group DEX. Analgesic consumption was significantly lower in group DEX (P=0.021). Mean arterial pressure during surgery, after laryngoscopy, and recovery was significantly lower in group DEX (P<0.05). The mean heart rate after laryngoscopy was significantly lower in group DEX (P=0.033). Conclusion: Intraoperative infusion of DEX improved hemodynamic parameters during surgery and decreased pain score, morphine use after lumbar laminectomy surgery.
背景和目的:急性术后疼痛仍然是腰椎板切除术患者的一个问题。我们比较了瑞芬太尼和右美托咪定对脊柱手术患者疼痛强度和镇痛需求的影响。方法:选取60例年龄在20 ~ 60岁的美国麻醉协会ASA 1级和2级患者择期腰椎椎板切除术进行随机临床试验研究。患者分为瑞芬太尼(R组)和右美托咪定(D组)两组，R组患者麻醉诱导前使用0.01 می g / kg / min瑞芬太尼。D组采用注射泵按µg / min / 50kg注射0.3右美托咪定。记录术后疼痛评分、恶心、呕吐、平均动脉血压、恢复时间、术后6、12、24小时心率。结果:DEX组异丙酚输注率明显低于R组(P = 0.001)。除术后6h外，DEX组疼痛评分明显降低。DEX组镇痛药用量显著低于对照组(P=0.021)。DEX组术中、喉镜检查后平均动脉压及恢复时间均显著低于对照组(P<0.05)。DEX组喉镜检查后平均心率明显低于对照组(P=0.033)。结论:术中输注DEX可改善术中血流动力学参数，降低腰椎椎板切除术后疼痛评分和吗啡使用。
{"title":"Comparison of the effect of dexmedetomidine with remifentanil on pain after lumbar laminectomy in patients under general anesthesia","authors":"S. Sane, Rahman Abasi vash, Farzaneh Joshaghani, Gonash Alizadeh, B. Gholamveisi, Parang Golabi","doi":"10.34172/mj.2021.058","DOIUrl":"https://doi.org/10.34172/mj.2021.058","url":null,"abstract":"Backgrounds and objectives: Acute postoperative pain is still an issue in patients undergoing lumbar laminectomy surgery. We compared the effects of remifentanil and dexmedetomidine on pain intensity as well as the analgesic requirements in patients undergoing spinal surgery. Methods: This randomized clinical trial study was conducted on 60 patients in the age range of 20 to 60 years with ASA class one and two of the American Anesthesia Association under elective lumbar vertebrae laminectomy. Patients were divided into two groups: Remifentanil (group R) and Dexmedetomidine (group D). Before induction of anesthesia in R group patients, 0.01 می g / kg / min remifentanil was used. In group D, 0.3 dexmedetomidine was injected from µg / min / 50kg using a syringe pump. Postoperative pain score, nausea, and vomiting, mean arterial blood pressure, and heart rate at recovery times, 6, 12, and 24 hours after surgery were recorded. Results: The propofol infusion rate was significantly lower in the DEX group than in group R (P = 0.001). Pain scores except for 6h after surgery was significantly lower in group DEX. Analgesic consumption was significantly lower in group DEX (P=0.021). Mean arterial pressure during surgery, after laryngoscopy, and recovery was significantly lower in group DEX (P<0.05). The mean heart rate after laryngoscopy was significantly lower in group DEX (P=0.033). Conclusion: Intraoperative infusion of DEX improved hemodynamic parameters during surgery and decreased pain score, morphine use after lumbar laminectomy surgery.","PeriodicalId":18474,"journal":{"name":"Medical journal of Tabriz University of Medical Sciences and Health Services","volume":"90 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-09-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"75719566","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
S. Dizaj, Masumeh Mokhtarpour, S. Z. Vahed, Simin Sharifi
Background: Scaffolds are one of the key components of tissue engineering that play an important role in the cell growth. The aim of the present study was to prepare hydroxyapatite-gelatin nanoscaffolds containing vanilla. Method: In this in vitro examination, the scaffolds were prepared using precipitation method. Then, vanilla was loaded into the prepared structures. Dynamic light scattering (DLS) and scanning electron microscopy (SEM) were used to evaluate the size and morphology of the scaffold. The surface charge of the scaffold (zeta potential) was also assessed by means of zeta sizer. The loading of vanilla was evaluated via ultraviolet–visible spectrophotometry in 372 nm. Results: The results showed that the scaffold was well prepared and had good physicochemical properties including appropriate particle size (110.23±0.42 nm), good particle distribution, high drug loading (65.03± 0.25%) and acceptable suspension stability (zeta potential equal to 36.42±0.80 mV). Descriptive statistics (mean ± SD) were used to report the results. Conclusion: Based on the obtained results of the current study, we suggested that the prepared scaffolds can be used for in vitro and in vivo applications in future studies for tissue engineering. More investigations are needed to test the usefulness of this scaffolds.
{"title":"Preparation and physicochemical assessment of hydroxyapatite-gelatin nanoscaffold containing vanilla","authors":"S. Dizaj, Masumeh Mokhtarpour, S. Z. Vahed, Simin Sharifi","doi":"10.34172/mj.2021.062","DOIUrl":"https://doi.org/10.34172/mj.2021.062","url":null,"abstract":"Background: Scaffolds are one of the key components of tissue engineering that play an important role in the cell growth. The aim of the present study was to prepare hydroxyapatite-gelatin nanoscaffolds containing vanilla. Method: In this in vitro examination, the scaffolds were prepared using precipitation method. Then, vanilla was loaded into the prepared structures. Dynamic light scattering (DLS) and scanning electron microscopy (SEM) were used to evaluate the size and morphology of the scaffold. The surface charge of the scaffold (zeta potential) was also assessed by means of zeta sizer. The loading of vanilla was evaluated via ultraviolet–visible spectrophotometry in 372 nm. Results: The results showed that the scaffold was well prepared and had good physicochemical properties including appropriate particle size (110.23±0.42 nm), good particle distribution, high drug loading (65.03± 0.25%) and acceptable suspension stability (zeta potential equal to 36.42±0.80 mV). Descriptive statistics (mean ± SD) were used to report the results. Conclusion: Based on the obtained results of the current study, we suggested that the prepared scaffolds can be used for in vitro and in vivo applications in future studies for tissue engineering. More investigations are needed to test the usefulness of this scaffolds.","PeriodicalId":18474,"journal":{"name":"Medical journal of Tabriz University of Medical Sciences and Health Services","volume":"58 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-09-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"89351591","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Masome Nobahar, M. Siahkouhian, S. Mirdar, Lily Tapak
Background: Asthma is a heterogeneous disease whose inflammatory processes are involved in the pathophysiology of atherosclerosis and endothelial dysfunction, and increases the prevalence of cardiovascular disease. This study was designed to investigate the effect of 8 weeks of interval training on some of the effective factors in atherosclerosis in heart tissue of asthmatic rats. Methods: In this experimental study, 15 rats were randomly divided into three groups of sham, asthma, and asthmatic-interval training. The model of asthma was induced by intraperitoneal injection of 1 ml ovalbumin for 2 weeks, and then asthmatic-interval training rats for 8 weeks participated in interval training. During the training protocol period, twice weekly intraperitoneal injections of ovalbumin 1 ml, and were placed into a glass container exposed to 5% ovalbumin spray. 48 hours after the last training session, the rats were anesthetized and their hearts were ejected. The levels of proteins expression of ICAM, VCAM, and galectin-3 were measured by immunohistochemistry, and western blotting, respectively. Data were analyzed using one-way ANOVA in SPSS at the significant level α=0.05. Results: The results of this study showed that the expression of ICAM (P=0.001), VCAM (P=0.001), and galectin-3 (P=0.001) was significantly increased in heart tissue of asthma rats. Whereas, interval training decreased the expression of ICAM (P=0.001), VCAM (P=0.001), and galectin-3 (P=0.01) proteins in the asthmatic-interval training group compared to the asthma group. Conclusions: It seems that, interval training is effective in preventing atherosclerosis in patients with asthma.
{"title":"A Survey of of interval training on the expression of ICAM, VCAM and galectin-3 proteins in the heart tissue of asthmatic rats","authors":"Masome Nobahar, M. Siahkouhian, S. Mirdar, Lily Tapak","doi":"10.34172/mj.2021.063","DOIUrl":"https://doi.org/10.34172/mj.2021.063","url":null,"abstract":"Background: Asthma is a heterogeneous disease whose inflammatory processes are involved in the pathophysiology of atherosclerosis and endothelial dysfunction, and increases the prevalence of cardiovascular disease. This study was designed to investigate the effect of 8 weeks of interval training on some of the effective factors in atherosclerosis in heart tissue of asthmatic rats. Methods: In this experimental study, 15 rats were randomly divided into three groups of sham, asthma, and asthmatic-interval training. The model of asthma was induced by intraperitoneal injection of 1 ml ovalbumin for 2 weeks, and then asthmatic-interval training rats for 8 weeks participated in interval training. During the training protocol period, twice weekly intraperitoneal injections of ovalbumin 1 ml, and were placed into a glass container exposed to 5% ovalbumin spray. 48 hours after the last training session, the rats were anesthetized and their hearts were ejected. The levels of proteins expression of ICAM, VCAM, and galectin-3 were measured by immunohistochemistry, and western blotting, respectively. Data were analyzed using one-way ANOVA in SPSS at the significant level α=0.05. Results: The results of this study showed that the expression of ICAM (P=0.001), VCAM (P=0.001), and galectin-3 (P=0.001) was significantly increased in heart tissue of asthma rats. Whereas, interval training decreased the expression of ICAM (P=0.001), VCAM (P=0.001), and galectin-3 (P=0.01) proteins in the asthmatic-interval training group compared to the asthma group. Conclusions: It seems that, interval training is effective in preventing atherosclerosis in patients with asthma.","PeriodicalId":18474,"journal":{"name":"Medical journal of Tabriz University of Medical Sciences and Health Services","volume":"13 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-09-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"78886276","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Hamed Masoumzadeh, Nasrin Hoseinzad, S. Jafari, A. Shayanfar, H. Vaez, Ommoleila Molavi
Background: STAT3 is an oncogenic signaling pathway found constitutively active in many types of human malignancies and plays a key role in cancer progression. Stattic is a small molecule, which selectively inhibits SH2 domain of STAT3. In most of the studies, stattic has been proposed as a promising strategy for inhibition of STAT3 in cancer cells harboring constitutively active STAT3. However, lack of proper formulation due to the poor water solubility and low bioavailability of stattic is a major limitation for its usage in clinic. The aim of this project was to develop poly(ethylene glycole)-block-poly(caprolactone) (PEG-b-PCL)-based polymeric micelles loaded with stattic and evaluate drug encapsulation efficiency and release in the developed formulations. Methods: In this experimental study, to prepare stattic loaded micellar formulations, co-solvent evaporation method was used. Mean diameter and polydispersity index (PDI) of micelles were defined by light scattering method. Encapsulated drug levels were measured using high performance liquid chromatography (HPLC). Data were analyzed using Graph pad prism software through one-way ANOVA analysis of variance. Results: Stattic was loaded in the polymeric micelles with encapsulation efficiency ranging from 40 to 73%. Drug loaded micelles were measured between 90 to 130 nm in size. PDI was obtained 0.3-1 and encapsulation of stattic in Polyethylene glycol-block-poly(α-benzyl carboxylate ε-caprolactone(PEG-b-PBCL) micellar formulation resulted in more than 6-fold increase in the water solubility of stattic (0.36 vs. 0.06 mg/mL). Respecting to high encapsulation efficiency, two micellar formulations were selected for further analysis that both of them released 70-80% of drug within the first hour, indicated burst release of drug. Conclusion: These findings show that PEG-b-PBCL copolymers can be a suitable vehicle for solubilization of stattic.
背景:STAT3是一种致癌信号通路，在许多类型的人类恶性肿瘤中被发现具有组成性活性，在癌症进展中起关键作用。Stattic是一种小分子，选择性抑制STAT3的SH2结构域。在大多数研究中，STAT3已被认为是一种很有前途的策略，可以抑制癌细胞中含有组成型活性STAT3的STAT3。然而，由于静液的水溶性差和生物利用度低，缺乏合适的配方是限制其临床应用的主要原因。本课题的目的是制备基于聚乙二醇-嵌段聚己内酯(PEG-b-PCL)的静态负载聚合物胶束，并评价其包封效率和释药效果。方法:采用共溶剂蒸发法制备静态负载胶束配方。用光散射法测定了胶束的平均直径和多分散度指数。采用高效液相色谱法测定包封药物的含量。数据采用Graph pad prism软件进行单因素方差分析。结果:静电剂被装入聚合物胶束，包封率为40% ~ 73%。载药胶束的尺寸在90 ~ 130 nm之间。PDI为0.3-1，聚乙二醇嵌段聚α-羧酸苄酯ε-己内酯(PEG-b-PBCL)胶束包封后，其水溶性提高了6倍以上(0.36 vs. 0.06 mg/mL)。考虑到较高的包封效率，我们选择了两种胶束配方进行进一步分析，这两种胶束配方均在第1小时内释放出药物的70-80%，显示出药物的爆发释放。结论:PEG-b-PBCL共聚物是一种较理想的溶液增溶载体。
{"title":"Development of polymeric nanoparticles loaded with STAT3 inhibitory, Stattic, for targeted cancer therapy","authors":"Hamed Masoumzadeh, Nasrin Hoseinzad, S. Jafari, A. Shayanfar, H. Vaez, Ommoleila Molavi","doi":"10.34172/mj.2021.060","DOIUrl":"https://doi.org/10.34172/mj.2021.060","url":null,"abstract":"Background: STAT3 is an oncogenic signaling pathway found constitutively active in many types of human malignancies and plays a key role in cancer progression. Stattic is a small molecule, which selectively inhibits SH2 domain of STAT3. In most of the studies, stattic has been proposed as a promising strategy for inhibition of STAT3 in cancer cells harboring constitutively active STAT3. However, lack of proper formulation due to the poor water solubility and low bioavailability of stattic is a major limitation for its usage in clinic. The aim of this project was to develop poly(ethylene glycole)-block-poly(caprolactone) (PEG-b-PCL)-based polymeric micelles loaded with stattic and evaluate drug encapsulation efficiency and release in the developed formulations. Methods: In this experimental study, to prepare stattic loaded micellar formulations, co-solvent evaporation method was used. Mean diameter and polydispersity index (PDI) of micelles were defined by light scattering method. Encapsulated drug levels were measured using high performance liquid chromatography (HPLC). Data were analyzed using Graph pad prism software through one-way ANOVA analysis of variance. Results: Stattic was loaded in the polymeric micelles with encapsulation efficiency ranging from 40 to 73%. Drug loaded micelles were measured between 90 to 130 nm in size. PDI was obtained 0.3-1 and encapsulation of stattic in Polyethylene glycol-block-poly(α-benzyl carboxylate ε-caprolactone(PEG-b-PBCL) micellar formulation resulted in more than 6-fold increase in the water solubility of stattic (0.36 vs. 0.06 mg/mL). Respecting to high encapsulation efficiency, two micellar formulations were selected for further analysis that both of them released 70-80% of drug within the first hour, indicated burst release of drug. Conclusion: These findings show that PEG-b-PBCL copolymers can be a suitable vehicle for solubilization of stattic.","PeriodicalId":18474,"journal":{"name":"Medical journal of Tabriz University of Medical Sciences and Health Services","volume":"43 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-09-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"79240381","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Background: The link between Frequency domain analysis of ground reaction forces and hearing loss and blind during walking is not well understood. Therefore, the purpose of this study was to investigate frequency domain analysis of ground reaction forces in deaf and blind people during walking. Methods: This study was quasi-experimental. The volunteer participants in current study consisted of thirty male that, were divided into three equal groups: blind, deaf and healthy (control) groups. Frequency Domain analysis of ground reaction forces in three groups was recorded by a foot scan system (sample rate: 300 Hz). The multivariate ANOVA test was used to compare between groups. The significance level was set at p < 0.05 for all analyses. Results: The results showed that, the frequency content with the power 99.5% in the vertical component of ground reaction forces in both deaf (p=0.020) and blind (p=0.021) groups reduced vs. control group. Also, frequency content with the power 99.5% in the Mid-foot in deaf (p=0.020) group was more than the blind group (p=0.036). Conclusion: The present study showed that the frequency content with the power 99.5% in the Mid-foot in deaf group was more than the blind group. It can be stated that the frequency domain analysis of ground reaction forces has the clinical value. Therefore, the use of therapeutic interventions to improve the frequency domain analysis of ground reaction forces in deaf and blind people is suggested.
{"title":"Frequency Domain analysis of Ground reaction forces in Deaf and blind people during walking","authors":"M. Barghamadi, Mohammad Abdollahpour Darvishani","doi":"10.34172/mj.2021.061","DOIUrl":"https://doi.org/10.34172/mj.2021.061","url":null,"abstract":"Background: The link between Frequency domain analysis of ground reaction forces and hearing loss and blind during walking is not well understood. Therefore, the purpose of this study was to investigate frequency domain analysis of ground reaction forces in deaf and blind people during walking. Methods: This study was quasi-experimental. The volunteer participants in current study consisted of thirty male that, were divided into three equal groups: blind, deaf and healthy (control) groups. Frequency Domain analysis of ground reaction forces in three groups was recorded by a foot scan system (sample rate: 300 Hz). The multivariate ANOVA test was used to compare between groups. The significance level was set at p < 0.05 for all analyses. Results: The results showed that, the frequency content with the power 99.5% in the vertical component of ground reaction forces in both deaf (p=0.020) and blind (p=0.021) groups reduced vs. control group. Also, frequency content with the power 99.5% in the Mid-foot in deaf (p=0.020) group was more than the blind group (p=0.036). Conclusion: The present study showed that the frequency content with the power 99.5% in the Mid-foot in deaf group was more than the blind group. It can be stated that the frequency domain analysis of ground reaction forces has the clinical value. Therefore, the use of therapeutic interventions to improve the frequency domain analysis of ground reaction forces in deaf and blind people is suggested.","PeriodicalId":18474,"journal":{"name":"Medical journal of Tabriz University of Medical Sciences and Health Services","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-09-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"80037910","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
A. Zare, Fatemeh Mehrasa, Afsaneh Radmehr, Lnaz Hashemizade
Abstract Introduction Chronic urticaria is a skin disorder characterized by swollen pruritic plaques and papules last long at least for 6-weeks. The aim of this study is to determine the anti TPO level in patients diagnosed with chronic urticaria, resistant to conventional treatments in order to test the possibility of thyroid hormone replacement with the aim of accelerating healing process. Materials and methods In this case-control study, 63 patients with chronic urticarial and 63 controls without chronic urticarial were studied bye anti TPO measurement.Anti TPO level upper than 40 IU/ has been recognized as positive. Results The prevalence of chronic urticaria among the case group was 3.5 times higher in women rather than men. Mean age of patients who have been diagnosed with chronic urticaria was 40.52±15.12 years. Mean Time of disease period was about 21.40±3.75 months. In this study, there has been no statistically significant association between age, Sex, TSH level and anti TPO concentration in both groups. Conclusion Anti TPO level in patients with chronic urticaria was not significantly different from the control group. However, Anti-TPO levels were significantly higher in patients with longer duration of disease.
{"title":"The level of anti-thyroperoxidase antibodies in chronic urticaria patients","authors":"A. Zare, Fatemeh Mehrasa, Afsaneh Radmehr, Lnaz Hashemizade","doi":"10.34172/mj.2021.064","DOIUrl":"https://doi.org/10.34172/mj.2021.064","url":null,"abstract":"Abstract Introduction Chronic urticaria is a skin disorder characterized by swollen pruritic plaques and papules last long at least for 6-weeks. The aim of this study is to determine the anti TPO level in patients diagnosed with chronic urticaria, resistant to conventional treatments in order to test the possibility of thyroid hormone replacement with the aim of accelerating healing process. Materials and methods In this case-control study, 63 patients with chronic urticarial and 63 controls without chronic urticarial were studied bye anti TPO measurement.Anti TPO level upper than 40 IU/ has been recognized as positive. Results The prevalence of chronic urticaria among the case group was 3.5 times higher in women rather than men. Mean age of patients who have been diagnosed with chronic urticaria was 40.52±15.12 years. Mean Time of disease period was about 21.40±3.75 months. In this study, there has been no statistically significant association between age, Sex, TSH level and anti TPO concentration in both groups. Conclusion Anti TPO level in patients with chronic urticaria was not significantly different from the control group. However, Anti-TPO levels were significantly higher in patients with longer duration of disease.","PeriodicalId":18474,"journal":{"name":"Medical journal of Tabriz University of Medical Sciences and Health Services","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-09-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"76255402","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Background: Leishmaniasis is a zoonotic disease and its the known as a health problem in all of the world. Case presentation: The patient, a 47-year-old man car driver from city of Bostan abad, with a history of traveling to cutaneous leishmaniasis endemic provinces last year, was referred to city health center with a 17 mm, no discharge and pus with necrotic appearance skin lesion. Biochemical, hematological parameters and urine culture and analysis were normal. Three smears was prepared with sterile vaccinostyle from the margins of wound and transferred to specific medium in sterile conditions. After staining the prepared smears from the wound, amastigotes with large nucleus and small kinetoplast in macrophages and active promastigotes in the liquid phase of medium were observed. Polymerase chain reaction (PCR) was performed using ITS-1 by specific primers. In the PCR product electrophoresis were compared with the marker bands and 350 bp band weight determined. With electrophoretic pattern and comparison with positive control band, isolates parasites belonged to Leishmania genus. In order to definitively diagnose the parasite species, sequencing method was used. The results showed 99% homology with Leishmania major. Finally, the patient with a diagnosis of rural cutaneous leishmaniasis was induction with Glucantime drug. Conclusion: Diagnosis of suspected cases with cutaneous leishmaniasis is the major importance. If an infected patient is diagnosed in the early stages of the disease, can be prevented the extent of the scar after recovery, as well as the possible complications of rural infection.
{"title":"Leishmania major cause of a cutaneous leishmaniasis in a car driver from east Azerbaijan province: A case report","authors":"M. Parsaei, Shalaleh Ganji, M. Khanmohammadi","doi":"10.34172/mj.2021.065","DOIUrl":"https://doi.org/10.34172/mj.2021.065","url":null,"abstract":"Background: Leishmaniasis is a zoonotic disease and its the known as a health problem in all of the world. Case presentation: The patient, a 47-year-old man car driver from city of Bostan abad, with a history of traveling to cutaneous leishmaniasis endemic provinces last year, was referred to city health center with a 17 mm, no discharge and pus with necrotic appearance skin lesion. Biochemical, hematological parameters and urine culture and analysis were normal. Three smears was prepared with sterile vaccinostyle from the margins of wound and transferred to specific medium in sterile conditions. After staining the prepared smears from the wound, amastigotes with large nucleus and small kinetoplast in macrophages and active promastigotes in the liquid phase of medium were observed. Polymerase chain reaction (PCR) was performed using ITS-1 by specific primers. In the PCR product electrophoresis were compared with the marker bands and 350 bp band weight determined. With electrophoretic pattern and comparison with positive control band, isolates parasites belonged to Leishmania genus. In order to definitively diagnose the parasite species, sequencing method was used. The results showed 99% homology with Leishmania major. Finally, the patient with a diagnosis of rural cutaneous leishmaniasis was induction with Glucantime drug. Conclusion: Diagnosis of suspected cases with cutaneous leishmaniasis is the major importance. If an infected patient is diagnosed in the early stages of the disease, can be prevented the extent of the scar after recovery, as well as the possible complications of rural infection.","PeriodicalId":18474,"journal":{"name":"Medical journal of Tabriz University of Medical Sciences and Health Services","volume":"117 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-09-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"84068299","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Nava Khalilinejad, Leila Mehdizadeh Fanid, N. Zeinalzadeh, Maedeh Alizadeh
Background: Attention deficit hyperactivity disorder (ADHD) is the most common and highly heritable psychiatric disorder among children which affects 3-7% of them all around the world. ADHD characterized by some symptoms such as extreme and inappropriate levels of motor activity, impulsivity and inattentiveness. Several studies have reported the association between dopaminergic pathway and ADHD. Dopamine D4 receptor gene and its variants is one of the major candidates that have been studied for ADHD. Here we investigated the genetic association between DRD4 rs1800955 polymorphism and ADHD among children from Northwest of Iran. Methods: This case-control study includes 200 ADHD children and 157 controls aged 6-12 years. ADHD children were diagnosed according to DSM-IV and Conners scale and were genotyped by PCR-Restriction fragment length polymorphism (RFLP) technique. Results: Frequencies of genotypes CC, TT and CT were 48 (24%), 15 (7.5%) and 137 (68.5%) in patient group and 50 (31.85% %), 7 (4.46%) and 100 (63.69 %) in control group respectively. According to results, there was no significant association between case and control groups (p>0.05). Conclusion: The results of this study indicate that there is no significant association between DRD4 rs1800955 polymorphism and attention deficit hyperactivity disorder among children from Northwest of Iran.
{"title":"Association between DRD4 rs1800955 polymorphism and Attention Deficit Hyperactivity Disorder susceptibility among children from Northwest of Iran: a case-control study","authors":"Nava Khalilinejad, Leila Mehdizadeh Fanid, N. Zeinalzadeh, Maedeh Alizadeh","doi":"10.34172/mj.2021.052","DOIUrl":"https://doi.org/10.34172/mj.2021.052","url":null,"abstract":"Background: Attention deficit hyperactivity disorder (ADHD) is the most common and highly heritable psychiatric disorder among children which affects 3-7% of them all around the world. ADHD characterized by some symptoms such as extreme and inappropriate levels of motor activity, impulsivity and inattentiveness. Several studies have reported the association between dopaminergic pathway and ADHD. Dopamine D4 receptor gene and its variants is one of the major candidates that have been studied for ADHD. Here we investigated the genetic association between DRD4 rs1800955 polymorphism and ADHD among children from Northwest of Iran. Methods: This case-control study includes 200 ADHD children and 157 controls aged 6-12 years. ADHD children were diagnosed according to DSM-IV and Conners scale and were genotyped by PCR-Restriction fragment length polymorphism (RFLP) technique. Results: Frequencies of genotypes CC, TT and CT were 48 (24%), 15 (7.5%) and 137 (68.5%) in patient group and 50 (31.85% %), 7 (4.46%) and 100 (63.69 %) in control group respectively. According to results, there was no significant association between case and control groups (p>0.05). Conclusion: The results of this study indicate that there is no significant association between DRD4 rs1800955 polymorphism and attention deficit hyperactivity disorder among children from Northwest of Iran.","PeriodicalId":18474,"journal":{"name":"Medical journal of Tabriz University of Medical Sciences and Health Services","volume":"77 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-06-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"85915908","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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