Mitochondrial DNA. Part B, Resources最新文献

We described the complete mitogenome sequence of Rhacophorus chenfui in this research. The circular mitogenome of R. chenfui is total length with 20,520 bp, encoded 39 genes (24 tRNA genes, 13 protein-coding genes, 2 rRNA genes) and two D-loop regions. The base composition of the mitogenome is 33.46% A, 30.80% T, 23.05% C, and 12.69% G. All tRNAs have the typical clover-leaf structure except for tRNA^Ser1(AGN) which have a reduced DHU arm. The results showed that R. chenfui is closely related with R. schlegelii, R. arboreus, Zhangixalus omeimontis and Z. dugritei. This work enriches the library of Rhacophoridae mitoenomes and provides a valuable resource for understanding the evolutionary history of Rhacophorus.

Lonicera microphylla Willd. ex Roem. & Schult. 1819 is a medicinal plant species, especially for treating chronic fever and dysentery. This study aimed to characterize the chloroplast genome of L. microphylla and reconstruct the phylogenetic relationships among Lonicera Linn. 1753. The circular complete chloroplast genome is 154,945 bp in length, with two inverted repeat regions (IRs; 23,841 bp), separated by a small single-copy region (SSC; 18,789 bp) and a large single-copy region (LSC; 88,474 bp). The overall GC content of L. microphylla plastome was 38.36%. The chloroplast genome encoded a total of 131 genes, including 84 protein-coding genes, 39 tRNA genes, and eight rRNA genes. Phylogenetic analysis reveals that L. microphylla was more closely related to Lonicera tangutica Maxim. 1878. Our result can provide reference for the phylogenetic relationship, resource development, and utilization of Lonicera.

Erodium stephanianum (Erodium stephanianum Willd. Sp. Pl. 1800) has not had its complete chloroplast genome reported, which limits our understanding of its genetics and evolution. In this study, we assembled and annotated its chloroplast genome, revealing a quadripartite structure with 76 protein-coding genes. Repeat analysis indicated the presence of simple sequence repeats. Phylogenetic analysis confirmed E. stephanianum's placement within the genus Erodium of the Geraniaceae family. These findings offer valuable genomic resources for comparative studies in Erodium and Geraniaceae, aiding genetic diversity and phylogenetic analyses.

In this study, the mitochondrial genome of Cylicocyclus ultrajectinus (Ihle, 1920) was sequenced for the first time using next-generation sequencing technology, and its compositional characteristics, structure, and phylogenetic relationship with other strongylid nematodes were analyzed by biological software. The results showed that these sequences contained 12 protein-coding genes, 22 tRNA genes, 2 rRNA genes, and 2 non-coding regions, all exhibiting a significant AT bias. Phylogenetic studies showed that C. ultrajectinus formed a distinct branch form other Cylicocyclus nematodes. This study contributes to the mitochondrial genome database of Strongylidae, laying a foundation for genetic variation, molecular classification, and evolutionary studies of strongylid nematodes.

Epicauta sibirica is both an important pest and an important economic insect. The mitogenome is 15,717 bp in length and consists of 13 protein-coding genes, 2 rRNA genes, 22 tRNA genes, and a control region. The nucleotide composition of E. sibirica is 37.0% of A, 18.3% of C, 11.3% of G, and 33.4% of T. The genus Epicauta was detected as a monophyletic group and more closely related to the genus Lytta than other genera within Meloidae. This study supplemented additional genetic data for future research on the phylogeny and evolution of Meloidae species.

The complete chloroplast genome of a Chinese endemic species Hemiboea subacaulis var. jiangxiensis was reported and characterized. The length of chloroplast genome is 153,311 bp, with 37.6% GC content. It has a typical quadripartite structure, including a large single-copy region (LSC, 84,287 bp), a small single-copy region (SSC, 18,094 bp) and a pair of inverted repeats (IRs, 25,465 bp). Totally, 133 unique genes were predicted, including 88 protein-coding genes, 37 tRNA genes and eight rRNA genes. Phylogenetic analysis and genetic distance revealed this species is sister to its typic H. subacaulis var. subacaulis. This study offers novel genetic data for the molecular identification and phylogenetic analysis of the Hemiboea.

We sequenced the complete mitochondrial genome of the aphid Periphyllus koelreuteriae. It is 16,828 bp long and includes 13 protein-coding genes (PCGs), 22 transfer RNA genes (tRNAs), two ribosome RNA genes (rRNAs), a control region, and a repeat region. The control region contains a 273-bp repeat motif repeated 2.3 times. The species-specific repeat region between trnE and trnF comprises two 340-bp repeat units. The maximum-likelihood tree based on all 37 mitochondrial genes indicated a close relationship between P. koelreuteriae and Periphyllus diacerivorus. This study provides a valuable mitogenomic resource for future research on Chaitophorinae phylogeny and P. koelreuteriae diversification.

Bidens aurea (Asteraceae), a native of tropical America is now widespread in Asia and the Americas. We explored the B. aurea chloroplast genome and conducted a phylogenetic analysis. The chloroplast genome was circular, consisting of a large single copy (LSC) of 83,909 base pairs (bp), a small single copy (SSC) of 18,407 bp, and two inverted repeat regions (IR) of 24,729 bp each. Phylogenetic analysis showed that the 19 Bidens taxa were divided into five major clades, and B. aurea was most closely related to two species. Our findings offer a high-quality B. aurea chloroplast genome, aiding DNA barcode development and evolutionary history reconstruction.

Phalaenopsis deliciosa (Rchb. f.), an ornamental orchid known for vibrant flowers, has a 148,090 bp chloroplast genome with 36.78% GC content. It includes an 85,241 bp large single-copy (LSC) region, an 11,649 bp small single-copy (SSC) region, and two 13,800 bp inverted repeats (IRs), encoding 122 genes (76 protein-coding, 38 tRNA, and 8 rRNA). This genome data refines the Phalaenopsis gene database and supports research on phylogeny and molecular breeding.

The complete mitochondrial genome of Mylabris sibirica Fischer von Waldheim, 1823 was sequenced and characterized. The mitogenome is 15,794 bp long with 37 annotated genes, comprising 13 protein-coding genes, 22 transfer RNA genes, and two ribosomal RNA genes. The phylogenetic analysis based on the mitochondrial genome sequences revealed that M. sibirica clustered with M. quadripunctata. This study presents the complete mitochondrial genome of M. sibirica for the first time, which could be beneficial for systematic studies on Mylabrini.