Mitochondrial DNA. Part B, Resources最新文献

This study sequenced and analyzed the complete mitochondrial genome (16,571 bp) of Schistura yingjiangensis, a species endemic to Yunnan, China. The genome contains 13 protein-coding genes, 22 tRNAs, and 2 rRNAs. Phylogenetic analysis revealed a close relationship between S. yingjiangensis, S. polytaenia, and S. longa. This work helps clarify the evolution of the large, paraphyletic genus Schistura and contributes to understanding the evolutionary dynamics of Cypriniformes.

Deep-sea organisms exhibit remarkable adaptations to extreme environmental conditions. It is important to elucidate the survival mechanisms of deep-sea life by exploring their genetic characteristics and origins. This study reports the complete mitochondrial genome of a deep-sea holothurian species (Benthodytes gosarsi). The mitochondrial genome of B. gosarsi is 17,133 bp in length and encodes 13 PCGs, 2 rRNAs, and 22 tRNAs. The heavy strand of B. gosarsi mtDNA consists of 31.62% A, 13.30% G, 37.38% T, and 17.70% C bases. Phylogenetic analysis with 18 mitogenomes from five sea cucumber orders determined the phylogenetic position of B. gosarsi within Holothuroidea. This study provides new insights into the genetic structure and phylogenetic relationships of B. gosarsi, contributing to a better understanding of deep-sea adaptation in holothurians.

Hiptage benghalensis (L.) Kurz 1874, an economically significant woody liana in the Malpighiaceae family, lacks a reported chloroplast genome, hindering its phylogenetic analysis. To address this, we sequenced and assembled the complete chloroplast genome of H. benghalensis, spanning 160,719 bp with an overall GC content of 36.7%, and containing 132 genes (87 protein-coding, 37 tRNA, and 8 rRNA). Phylogenetic analysis revealed that H. benghalensis exhibits the closest evolutionary relationship with species from the genus Aspidopterys. This study provides the first complete chloroplast genome for H. benghalensis, enhancing our understanding of its evolution and potential applications within the Malpighiaceae family.

In this study, the complete mitochondrial genomes of four Xanthoparmelia species (X. chlorochroa, X. coreana, X. pulvinaris, and X. stenophylla) were sequenced, assembled and annotated. The four mitochondrial genomes are all composed of circular DNA molecules, with a total length ranging from 81,294 bp to 88,296 bp, containing 41-42 genes (14 protein-coding genes (PCGs), 2 rRNA genes, and 25-26 tRNA genes), the GC content is 30%-30.7% and the AT skew is positive. Phylogenetic analysis based on 14 PCGs and rRNA genes revealed that the four Xanthoparmelia species form a well-supported clade within Parmeliaceae.

Barathrites iris Zugmayer, 1911 is an abyssal fish species inhabiting depths exceeding 3,000 m. We successfully sequenced and characterized the complete mitochondrial genome of B. iris, which represents a closed circular molecule of 16,646 bp with a GC content of 48.98%. Phylogenetic analysis of Ophidiiformes based on whole mitochondrial genome sequences demonstrated that B. iris forms a sister group with Penopus microphthalmus while supporting the polyphyly of Bythitidae as well as the paraphyletic status of Ophidioidei. As the first report of a complete mitochondrial genome for B. iris, this study provides valuable genomic resources for investigating deep-sea adaptation mechanisms in Ophidiiformes and contributes to our understanding of evolutionary patterns in abyssal teleost fishes.

This study presents the first mitochondrial genome (16,342 bp) of the invasive species Cladonema digitatum Fang et al., 2022, revealing a conserved Capitata-like gene arrangement, comprising 13 protein-coding genes, two rRNA genes, and two tRNA genes, along with a duplicated pseudo-cox1 fragment. Phylogenetic analysis robustly supported C. digitatum and Cladonema multiramosum as reciprocally monophyletic mitogenome lineages, forming a robust clade sister to Cladonema pacificum. This topology supports the morphological dichotomy between the Cladonema radiatum-like lineage (filiform tentacles, gastric pouches) and C. pacificum-like species. These findings contribute a critical genomic resource for advancing the understanding of medusozoan phylogeny.

Phyllostachys lithophila Hayata 1916 is a unique bamboo species endemic to Taiwan, typically found at elevations ranging from 500 to 1,500 meters. This study provides a detailed analysis of the complete chloroplast genome of P. lithophila for the first time. The genome spans 139,664 base pairs (bp) and consists of a large single-copy (LSC) region of 83,192 bp, a small single-copy (SSC) region of 12,869 bp, and two inverted repeat (IR) regions, each 21,798 bp in length. The plastid genome encodes a total of 129 genes, including 83 protein-coding genes, 38 transfer RNA (tRNA) genes, and 8 ribosomal RNA (rRNA) genes. Phylogenetic analysis confirms that P. lithophila shares a closer phylogenetic relationship with species of Phyllostachys within the Arundinarieae tribe, while being phylogenetically distinct from the morphologically similar P. makinoi.

Scrophularia marilandica Linnaeus 1753 is a herbaceous perennial medicinal plant of the family Scrophulariaceae, native throughout eastern and central North America. In this study, the first complete chloroplast genome of S. marilandica was reported and phylogenetic analysis was conducted with other 11 species from Scrophulariaceae. The chloroplast genome was 152,414 bp with 132 genes and includes a large single-copy (LSC) region (83,583 bp), a small single-copy (SSC) region (17,925 bp), and a pair of inverted repeat (IRs) regions (25,453 bp). The chloroplast genome reported here will be beneficial for its interspecific identification and evolutionary studies of Scrophularia.

Leptomastidea (Hymenoptera, Encyrtidae) are parasitoids of agricultural/forestry mealybug pests. This study first sequences and annotates L. bifasciata's complete mitochondrial genome (15,768 bp), which contains 13 PCGs, 22 tRNAs, 2 rRNAs. All 13 PCGs start with ATN (ATT, ATG, ATA); most end with TAA, except ND1 (TAG). Phylogenetic analysis shows L. bifasciata is sister to Anagyrus spp.

We have sequenced the complete mitochondrial genome of the firefly Luciola parvula Kiesenwetter, 1874 (Coleoptera, Lampyridae, Luciolinae) from Niigata Prefecture, Honshu, Japan, and inferred the Luciolinae phylogeny. The circular genome of 16,685 bp (GenBank accession number, LC677171) has a base composition of A (43.52%), C (13.21%), G (8.52%) and T (34.74%). Our sequence is similar to other Metazoa, which contains 13 protein coding genes (PCGs), 2 rRNA genes, 22 tRNA genes and D-loop. Molecular phylogenetic analyses based on mitochondrial whole genome sequences revealed L. parvula as a sister group of (L. unmunsana [MT134039] + L. parvula [OL944082]). Identity of the two L. parvula sequences (LC677171 and OL944082) is only 90.5% (14,064/15,537 bp), suggesting the two L. parvula might be different species. Further taxonomical examination including morphological analyses should be required to test if the two populations are different species.