Pub Date : 2024-03-15DOI: 10.31276/vjste.66(1).34-45
Huong Ngoc Thi Dao, T. Nguyen, Tran Huyen Thi Nguyen, Thuy Hong Ngoc Phan, Phuong Hoang Tran
1-(4-sulfobutyl)pyridinium chlorozincate ionic liquid was synthesised in high yield (89%) through a simple route and characterised using FT-IR, NMR, and HRMS spectroscopy. The one-pot reaction was employed for the condensation reaction of benzaldehydes, 5,5-dimethyl-1,3-cyclohexanedione, and dicyanomethane, utilising the prepared ionic liquid catalyst. The optimal conditions for the reaction were achieved with a catalyst loading of 10 mol%, a solvent mixture of water and ethanol (1:1, v/v), maintained at 100°C for 180 minutes. A broad spectrum of benzopyran-5(6H)-one derivatives was prepared, yielding moderate to good results. The notable features of this procedure include readily available substrates, short reaction times, the use of environmentally friendly solvents, and the elimination of the need for chromatography in the isolation of products. Anew acidic pyridinium-based ionic liquid was successfully synthesised and used as a catalyst in the synthetic process of 2-amino-3-cyano-4-phenyl-7,7-dimethyl-7,8-dihydro-4H-1-benzopyran-5(6H)-ones. These reactions involved a condensation reaction of aromatic benzaldehydes, 5,5-dimethyl-1,3-cyclohexanedione, and dicyanomethane in a solvent mixture of ethanol and water, resulting in the desired products in moderate to good yields. The procedure is efficient and eco-friendly, and the products can be easily obtained by recrystallisation. Thus, this protocol offers an alternative to existing methods.
{"title":"Preparation of pyridinium-based ionic liquid and application as a green catalyst for the synthetic route of 4H-1-benzopyran-5(6H)-ones","authors":"Huong Ngoc Thi Dao, T. Nguyen, Tran Huyen Thi Nguyen, Thuy Hong Ngoc Phan, Phuong Hoang Tran","doi":"10.31276/vjste.66(1).34-45","DOIUrl":"https://doi.org/10.31276/vjste.66(1).34-45","url":null,"abstract":"1-(4-sulfobutyl)pyridinium chlorozincate ionic liquid was synthesised in high yield (89%) through a simple route and characterised using FT-IR, NMR, and HRMS spectroscopy. The one-pot reaction was employed for the condensation reaction of benzaldehydes, 5,5-dimethyl-1,3-cyclohexanedione, and dicyanomethane, utilising the prepared ionic liquid catalyst. The optimal conditions for the reaction were achieved with a catalyst loading of 10 mol%, a solvent mixture of water and ethanol (1:1, v/v), maintained at 100°C for 180 minutes. A broad spectrum of benzopyran-5(6H)-one derivatives was prepared, yielding moderate to good results. The notable features of this procedure include readily available substrates, short reaction times, the use of environmentally friendly solvents, and the elimination of the need for chromatography in the isolation of products. Anew acidic pyridinium-based ionic liquid was successfully synthesised and used as a catalyst in the synthetic process of 2-amino-3-cyano-4-phenyl-7,7-dimethyl-7,8-dihydro-4H-1-benzopyran-5(6H)-ones. These reactions involved a condensation reaction of aromatic benzaldehydes, 5,5-dimethyl-1,3-cyclohexanedione, and dicyanomethane in a solvent mixture of ethanol and water, resulting in the desired products in moderate to good yields. The procedure is efficient and eco-friendly, and the products can be easily obtained by recrystallisation. Thus, this protocol offers an alternative to existing methods.","PeriodicalId":18650,"journal":{"name":"Ministry of Science and Technology, Vietnam","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2024-03-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140240388","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-03-15DOI: 10.31276/vjste.66(1).96-103
Van-Hoai Bui, Hong-Tham N Vo, Thanh-Tuan Duong, Se-Kwon Kim, Dai-Nghiep Ngo
The aim of the present study is to investigate the anti-inflammatory effect of gallic acid grafted onto COS chains (GA-COS), focusing on the reduction of nitric oxide production, downregulation of inflammatory signals such as inducible nitric oxide synthase (iNOS), gene expression of cytokines such as TNF-α, IL-1β, IL-6, and nuclear factor kappa B (NF-κB) signalling, including the p50 and p65 subunits. The anti-inflammatory effect is mediated through the reduction of nitric oxide production and downregulation of inflammatory proteins such as inducible nitric oxide synthase (iNOS), gene expression of cytokines like TNF-α, IL-1β, IL-6, and nuclear factor kappa B (NF-κB) signalling, including the p50 and p65 subunits. Target proteins were identified by western blot analysis with specific monoclonal antibodies. The levels of gene expression were determined by the RT-PCR method. The results demonstrate that GA-COS effectively reduces nitric oxide generation and downregulates iNOS protein and cytokine expression and NF-κB signalling in lipopolysaccharide (LPS) -induced RAW 264.7 cells. GA-COS exhibits significantly enhanced anti-inflammatory activity compared to the free chitooligosaccharide chain. This study lays the groundwork for future research to demonstrate that GA-COS holds significant potential as a novel compound for the prevention of inflammatory diseases.
{"title":"Anti-inflammatory effect of gallic acid-conjugated chitooligosaccharides in lipopolysaccharide-stimulated RAW 264.7 macrophages","authors":"Van-Hoai Bui, Hong-Tham N Vo, Thanh-Tuan Duong, Se-Kwon Kim, Dai-Nghiep Ngo","doi":"10.31276/vjste.66(1).96-103","DOIUrl":"https://doi.org/10.31276/vjste.66(1).96-103","url":null,"abstract":"The aim of the present study is to investigate the anti-inflammatory effect of gallic acid grafted onto COS chains (GA-COS), focusing on the reduction of nitric oxide production, downregulation of inflammatory signals such as inducible nitric oxide synthase (iNOS), gene expression of cytokines such as TNF-α, IL-1β, IL-6, and nuclear factor kappa B (NF-κB) signalling, including the p50 and p65 subunits. The anti-inflammatory effect is mediated through the reduction of nitric oxide production and downregulation of inflammatory proteins such as inducible nitric oxide synthase (iNOS), gene expression of cytokines like TNF-α, IL-1β, IL-6, and nuclear factor kappa B (NF-κB) signalling, including the p50 and p65 subunits. Target proteins were identified by western blot analysis with specific monoclonal antibodies. The levels of gene expression were determined by the RT-PCR method. The results demonstrate that GA-COS effectively reduces nitric oxide generation and downregulates iNOS protein and cytokine expression and NF-κB signalling in lipopolysaccharide (LPS) -induced RAW 264.7 cells. GA-COS exhibits significantly enhanced anti-inflammatory activity compared to the free chitooligosaccharide chain. This study lays the groundwork for future research to demonstrate that GA-COS holds significant potential as a novel compound for the prevention of inflammatory diseases.","PeriodicalId":18650,"journal":{"name":"Ministry of Science and Technology, Vietnam","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2024-03-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140240671","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-03-15DOI: 10.31276/vjste.66(1).24-33
Thi Lan Phung, Thi Kim Giang Nguyen, Phuong Hien Ho, Thanh Nga Pham
In this study, cellulose was produced by activating peanut shells with 5% NaOH as an alkaline agent and bleaching with 5 wt.% H2O2. Before being modified with chitosan, the cellulose was slightly oxidized with ammonium persulfate to introduce carboxyl groups. The equilibrium and kinetics of nickel adsorption on the as-prepared material were investigated. Langmuir, Freundlich, and Temkin isotherm models were used to describe the equilibrium isotherms. The Langmuir and Temkin adsorption isotherms were better fits to the equilibrium data than the Freundlich equation. The Langmuir monolayer adsorption capacity of nickel ions on cellulose from peanut shells modified with chitosan was found to be 25.70 mg/g. The Temkin adsorption constant was calculated as 0.45 kJ/mol. Therefore, the interaction between nickel ions and the surface of the as-prepared material are assumed to be weak. Kinetic data were evaluated by pseudo-first-order and pseudo-second-order models, and rate constants were determined. Simulations demonstrated that the pseudo-second-order equation could adequately describe the adsorption of nickel ions.
{"title":"Study on the equilibrium and kinetics of nickel (II) adsorption on cellulose from peanut shell modified with chitosan","authors":"Thi Lan Phung, Thi Kim Giang Nguyen, Phuong Hien Ho, Thanh Nga Pham","doi":"10.31276/vjste.66(1).24-33","DOIUrl":"https://doi.org/10.31276/vjste.66(1).24-33","url":null,"abstract":"In this study, cellulose was produced by activating peanut shells with 5% NaOH as an alkaline agent and bleaching with 5 wt.% H2O2. Before being modified with chitosan, the cellulose was slightly oxidized with ammonium persulfate to introduce carboxyl groups. The equilibrium and kinetics of nickel adsorption on the as-prepared material were investigated. Langmuir, Freundlich, and Temkin isotherm models were used to describe the equilibrium isotherms. The Langmuir and Temkin adsorption isotherms were better fits to the equilibrium data than the Freundlich equation. The Langmuir monolayer adsorption capacity of nickel ions on cellulose from peanut shells modified with chitosan was found to be 25.70 mg/g. The Temkin adsorption constant was calculated as 0.45 kJ/mol. Therefore, the interaction between nickel ions and the surface of the as-prepared material are assumed to be weak. Kinetic data were evaluated by pseudo-first-order and pseudo-second-order models, and rate constants were determined. Simulations demonstrated that the pseudo-second-order equation could adequately describe the adsorption of nickel ions.","PeriodicalId":18650,"journal":{"name":"Ministry of Science and Technology, Vietnam","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2024-03-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140239664","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-03-15DOI: 10.31276/vjste.66(1).10-15
Nguyen Tri Tuan, T. Tam, Nguyen Tu, D. Q. Trung, N. V. Du, Tran Minh Tien, Vu Thi Hang, Nguyen Trong Tuan, N. V. Quang, M. Tran
In this study, we successfully synthesized europium-doped TiO2 (TiO2:Eu3+) nanomaterials (NMs) with crystalline sizes ranging from 6.5 to 71.7 nm through a sol-gel method. Analysis of X-ray diffraction (XRD) patterns and ultraviolet-visible (UV-Vis) spectra indicates that the substitution of smaller-sized Ti4+ ions (0.745 Å) by larger-sized Eu3+ ions (0.947 Å) was more efficient at higher doping concentrations and annealing temperatures. Photoluminescence (PL) and photoluminescence excitation (PLE) spectra of the synthesized TiO2:7%Eu3+NMs exhibit a strong red emission band peaking at 613 nm and remarkable absorption in the blue light region centered at 463 nm, making them suitable for use in white light-emitting diode (WLED) applications. Our findings reveal that both annealing temperature and doping concentration significantly influence the structure and properties of these materials. Besides, the research team has seen, under the experimental conditions, the TiO2:7%Eu3+ sample annealed at 800°C exhibits the highest PL intensity. These results underscore the potential of the synthesized TiO2:Eu3+ NMs as red-emitting materials for WLED applications.
{"title":"Effect of annealing temperature and doping concentrations on structure and optical properties of Eu3+-doped TiO2 nanomaterials","authors":"Nguyen Tri Tuan, T. Tam, Nguyen Tu, D. Q. Trung, N. V. Du, Tran Minh Tien, Vu Thi Hang, Nguyen Trong Tuan, N. V. Quang, M. Tran","doi":"10.31276/vjste.66(1).10-15","DOIUrl":"https://doi.org/10.31276/vjste.66(1).10-15","url":null,"abstract":"In this study, we successfully synthesized europium-doped TiO2 (TiO2:Eu3+) nanomaterials (NMs) with crystalline sizes ranging from 6.5 to 71.7 nm through a sol-gel method. Analysis of X-ray diffraction (XRD) patterns and ultraviolet-visible (UV-Vis) spectra indicates that the substitution of smaller-sized Ti4+ ions (0.745 Å) by larger-sized Eu3+ ions (0.947 Å) was more efficient at higher doping concentrations and annealing temperatures. Photoluminescence (PL) and photoluminescence excitation (PLE) spectra of the synthesized TiO2:7%Eu3+NMs exhibit a strong red emission band peaking at 613 nm and remarkable absorption in the blue light region centered at 463 nm, making them suitable for use in white light-emitting diode (WLED) applications. Our findings reveal that both annealing temperature and doping concentration significantly influence the structure and properties of these materials. Besides, the research team has seen, under the experimental conditions, the TiO2:7%Eu3+ sample annealed at 800°C exhibits the highest PL intensity. These results underscore the potential of the synthesized TiO2:Eu3+ NMs as red-emitting materials for WLED applications.","PeriodicalId":18650,"journal":{"name":"Ministry of Science and Technology, Vietnam","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2024-03-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140240391","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-03-15DOI: 10.31276/vjste.66(1).03-09
Nguyen Chi Cuong, T. X. Thang, Lam Minh Thinh, Vuong Dinh Duy Phuc, Phan Minh Duc Truong, Truong Huu Ly, Ngo Vo Ke Thanh, Le Quoc Cuong
At atmospheric pressure (p=101325 Pa), the effects of humidity and temperature on moist air become important when discussing the quality factor of micro-cantilever and micro-bridge resonators. The squeeze film damping (SFD) problem, the dominant damping source for micro-beam resonators, is modelled using the modified molecular gas lubrication (MMGL) equation with finite element modelling (FEM) in the eigenvalue problem. The MMGL equation is modified with the effective viscosity of moist air (μeff) to account for the effects of humidity and temperature. Other damping sources, such as thermoelastic damping (TED) and the support loss of micro-beam resonators, are also calculated. The quality factor of micro-beam resonators is then discussed over a wide range of temperatures and relative humidity levels at atmospheric pressure and gas rarefaction. The results show that the quality factor of micro-cantilever and micro-bridge resonators increases as both humidity and temperature rise in atmospheric pressure and gas rarefaction. Furthermore, the quality factor of a micro-bridge resonator with changes in humidity and temperature is significantly higher than that of a micro-cantilever resonator in atmospheric pressure and gas rarefaction.
{"title":"Effects of humidity and temperature on quality factor of micro-beam resonators in atmospheric pressure and gas rarefaction","authors":"Nguyen Chi Cuong, T. X. Thang, Lam Minh Thinh, Vuong Dinh Duy Phuc, Phan Minh Duc Truong, Truong Huu Ly, Ngo Vo Ke Thanh, Le Quoc Cuong","doi":"10.31276/vjste.66(1).03-09","DOIUrl":"https://doi.org/10.31276/vjste.66(1).03-09","url":null,"abstract":"At atmospheric pressure (p=101325 Pa), the effects of humidity and temperature on moist air become important when discussing the quality factor of micro-cantilever and micro-bridge resonators. The squeeze film damping (SFD) problem, the dominant damping source for micro-beam resonators, is modelled using the modified molecular gas lubrication (MMGL) equation with finite element modelling (FEM) in the eigenvalue problem. The MMGL equation is modified with the effective viscosity of moist air (μeff) to account for the effects of humidity and temperature. Other damping sources, such as thermoelastic damping (TED) and the support loss of micro-beam resonators, are also calculated. The quality factor of micro-beam resonators is then discussed over a wide range of temperatures and relative humidity levels at atmospheric pressure and gas rarefaction. The results show that the quality factor of micro-cantilever and micro-bridge resonators increases as both humidity and temperature rise in atmospheric pressure and gas rarefaction. Furthermore, the quality factor of a micro-bridge resonator with changes in humidity and temperature is significantly higher than that of a micro-cantilever resonator in atmospheric pressure and gas rarefaction.","PeriodicalId":18650,"journal":{"name":"Ministry of Science and Technology, Vietnam","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2024-03-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140238194","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-03-15DOI: 10.31276/vjste.66(1).77-83
Tien Hai Ly, Le Hoang Vu, Lý Văn Khánh, Nguyen Thi Ngoc Anh
This study was conducted to assess the effects of stocking densities on the water quality, survival and growth of the freshwater prawn Macrobrachium rosenbergii postlarvae (PL) cultured in a biofloc system integrated with red seaweed (Gracilaria tenuistipitata). Postlarvae prawns weighing 0.012±0.001 g were stocked at densities of 1,000, 1,500, 2,000, and 2,500 PL/m3, noted as D1, D1.5, D2, and D2.5, respectively. Each density treatment was replicated three times and randomly assigned to 12 plastic tanks of 150 l at a salinity of 10 ppt. Red seaweed was added at a rate of 1.5 kg/m3, and molasses was used as a carbon source to maintain a C:N ratio of 15:1. No water exchange occurred during the 30-day rearing period. Results indicated that water quality parameters, including TAN and NO2-, biofloc volume, total heterotrophic bacteria, and Vibrio spp. counts, increased at higher prawn densities but remained within the appropriate range for prawn performance. Growth rate in weight and survival of prawns decreased as stocking density increased, with D1 and D1.5 showing comparable and significantly higher results compared to D2 and D2.5. Prawn production increased with rising stocking density, with a significant difference among treatments (p<0.05). Furthermore, prawns in D1 and D1.5 exhibited significantly higher feed efficiency than those in D2 and D2.5. These findings suggest that nursery rearing of prawn PL at a density of 1,500 ind/m3 in a biofloc system integrated with red seaweed offers optimal growth and feed efficiency, while maintaining good water quality and conserving water resources.
{"title":"Nursery rearing freshwater prawn, Macrobrachium rosenbergii, in biofloc system integrated with red seaweed, Gracilaria tenuistipitata, at different stocking densities under zero water exchange","authors":"Tien Hai Ly, Le Hoang Vu, Lý Văn Khánh, Nguyen Thi Ngoc Anh","doi":"10.31276/vjste.66(1).77-83","DOIUrl":"https://doi.org/10.31276/vjste.66(1).77-83","url":null,"abstract":"This study was conducted to assess the effects of stocking densities on the water quality, survival and growth of the freshwater prawn Macrobrachium rosenbergii postlarvae (PL) cultured in a biofloc system integrated with red seaweed (Gracilaria tenuistipitata). Postlarvae prawns weighing 0.012±0.001 g were stocked at densities of 1,000, 1,500, 2,000, and 2,500 PL/m3, noted as D1, D1.5, D2, and D2.5, respectively. Each density treatment was replicated three times and randomly assigned to 12 plastic tanks of 150 l at a salinity of 10 ppt. Red seaweed was added at a rate of 1.5 kg/m3, and molasses was used as a carbon source to maintain a C:N ratio of 15:1. No water exchange occurred during the 30-day rearing period. Results indicated that water quality parameters, including TAN and NO2-, biofloc volume, total heterotrophic bacteria, and Vibrio spp. counts, increased at higher prawn densities but remained within the appropriate range for prawn performance. Growth rate in weight and survival of prawns decreased as stocking density increased, with D1 and D1.5 showing comparable and significantly higher results compared to D2 and D2.5. Prawn production increased with rising stocking density, with a significant difference among treatments (p<0.05). Furthermore, prawns in D1 and D1.5 exhibited significantly higher feed efficiency than those in D2 and D2.5. These findings suggest that nursery rearing of prawn PL at a density of 1,500 ind/m3 in a biofloc system integrated with red seaweed offers optimal growth and feed efficiency, while maintaining good water quality and conserving water resources.","PeriodicalId":18650,"journal":{"name":"Ministry of Science and Technology, Vietnam","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2024-03-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140241455","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
In the present study, we investigated the antifungal activity of a methanolic extract of Glycyrrhiza uralensisFisch. root, which inhibited the growth of the plant pathogens Fusarium oxysporum and Phytophthora capsici. The EC50 values of the G. uralensis Fisch. extract three, six, and nine days after treatment were 113.4, 150.6, and 191.4 μg/ml for F. oxysporum and 84.7, 148.6, and 190.1 μg/ml for P. capsici, respectively, and the antifungal activity exhibited a dose-dependent response. The MIC value of G. uralensis Fisch. extract was found to be 100, 200, and 400 μg/ml at three, six, and nine days after treatment for F. oxysporum. In contrast, for P. capsici, the MIC value was 50, 100, and 100 μg/ml at three, six, and nine days after treatment. After three days of incubation with 400 μg/ml G. uralensis Fisch. extract, the hyphae of F. oxysporum exhibited abnormalities and atrophy, leading to mycelial collapse. In contrast, the hyphae of P. capsici displayed swelling with increased branching. These results furnish valuable insights into the antifungal metabolites present in G. uralensis Fisch. extract, contributing to the development of environmentally friendly products aimed at controlling the growth of pathogenic fungi.
在本研究中，我们研究了甘草根甲醇提取物的抗真菌活性，它能抑制植物病原体 Fusarium oxysporum 和 Phytophthora capsici 的生长。G. uralensis Fisch.提取物在处理后 3 天、6 天和 9 天对 F. oxysporum 的 EC50 值分别为 113.4、150.6 和 191.4 μg/ml，对 P. capsici 的 EC50 值分别为 84.7、148.6 和 190.1 μg/ml，抗真菌活性呈剂量依赖性反应。G. uralensis Fisch.提取物在处理后 3、6 和 9 天对 F. oxysporum 的 MIC 值分别为 100、200 和 400 μg/ml。相比之下，在处理后 3 天、6 天和 9 天，荚膜穗霉的 MIC 值分别为 50、100 和 100 μg/ml。用 400 μg/ml G. uralensis Fisch.提取物培养三天后，F. oxysporum 的菌丝出现异常和萎缩，导致菌丝崩溃。与此相反，P. capsici 的菌丝膨胀，分枝增多。这些结果为了解 G. uralensis Fisch.提取物中的抗真菌代谢物提供了宝贵的信息，有助于开发旨在控制病原真菌生长的环境友好型产品。
{"title":"Antifungal activity of a methanolic extract of Glycyrrhiza uralensis Fisch. root against Fusarium oxysporum and Phytophthora capsici","authors":"Dang-Minh-Chanh Nguyen, Thi-Hoan Luong, Van-Viet Nguyen, Woo-Jin Jung","doi":"10.31276/vjste.66(1).46-52","DOIUrl":"https://doi.org/10.31276/vjste.66(1).46-52","url":null,"abstract":"In the present study, we investigated the antifungal activity of a methanolic extract of Glycyrrhiza uralensisFisch. root, which inhibited the growth of the plant pathogens Fusarium oxysporum and Phytophthora capsici. The EC50 values of the G. uralensis Fisch. extract three, six, and nine days after treatment were 113.4, 150.6, and 191.4 μg/ml for F. oxysporum and 84.7, 148.6, and 190.1 μg/ml for P. capsici, respectively, and the antifungal activity exhibited a dose-dependent response. The MIC value of G. uralensis Fisch. extract was found to be 100, 200, and 400 μg/ml at three, six, and nine days after treatment for F. oxysporum. In contrast, for P. capsici, the MIC value was 50, 100, and 100 μg/ml at three, six, and nine days after treatment. After three days of incubation with 400 μg/ml G. uralensis Fisch. extract, the hyphae of F. oxysporum exhibited abnormalities and atrophy, leading to mycelial collapse. In contrast, the hyphae of P. capsici displayed swelling with increased branching. These results furnish valuable insights into the antifungal metabolites present in G. uralensis Fisch. extract, contributing to the development of environmentally friendly products aimed at controlling the growth of pathogenic fungi.","PeriodicalId":18650,"journal":{"name":"Ministry of Science and Technology, Vietnam","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2024-03-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140238533","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-03-15DOI: 10.31276/vjste.66(1).120-128
Linh-Thy Le, Khanh-Bang V. Quang, Trieu-Vi Vo, Thanh-Mai T. Nguyen, Thi-Viet-Huong Dao, Xuan-Thanh Bui
Air pollution is one of the leading risk factors for death but also a significant contributor to the global disease burden, affecting quality of life. According to a World Health Organisation (WHO) report, seven million people die from air pollution every year, and 9 out of 10 people worldwide breathe polluted air. Any person can be affected by exposure to polluted air, especially the elderly, children, pregnant women, and people with comorbidities. Some studies indicate that the diseases most affected by air pollution are respiratory infections, chronic obstructive pulmonary disease (COPD), lung cancer, and cardiovascular diseases. The degree of effect on the body depends on the pollutant composition, source and dose, level and duration of exposure to polluted air. Particulate matter (PM10 and PM2.5), carbon monoxide (CO), ozone (O3), nitrogen dioxide (NO2), and sulphur dioxide (SO2) could lead to air pollution. Long-term exposure to air pollution can affect every organ in the body and worsen existing health conditions. Short-term exposure to contaminants can include unpleasant sensations such as coughing, wheezing, shortness of breath, eye, nose, and throat irritation, headache, dizziness, and fatigue. Community and individual solutions such as using clean fuel, wearing personal masks, filtering indoor air, and ventilating need to be taken to reduce the impact of air pollution.
{"title":"Environmental and health impacts of air pollution: A mini-review","authors":"Linh-Thy Le, Khanh-Bang V. Quang, Trieu-Vi Vo, Thanh-Mai T. Nguyen, Thi-Viet-Huong Dao, Xuan-Thanh Bui","doi":"10.31276/vjste.66(1).120-128","DOIUrl":"https://doi.org/10.31276/vjste.66(1).120-128","url":null,"abstract":"Air pollution is one of the leading risk factors for death but also a significant contributor to the global disease burden, affecting quality of life. According to a World Health Organisation (WHO) report, seven million people die from air pollution every year, and 9 out of 10 people worldwide breathe polluted air. Any person can be affected by exposure to polluted air, especially the elderly, children, pregnant women, and people with comorbidities. Some studies indicate that the diseases most affected by air pollution are respiratory infections, chronic obstructive pulmonary disease (COPD), lung cancer, and cardiovascular diseases. The degree of effect on the body depends on the pollutant composition, source and dose, level and duration of exposure to polluted air. Particulate matter (PM10 and PM2.5), carbon monoxide (CO), ozone (O3), nitrogen dioxide (NO2), and sulphur dioxide (SO2) could lead to air pollution. Long-term exposure to air pollution can affect every organ in the body and worsen existing health conditions. Short-term exposure to contaminants can include unpleasant sensations such as coughing, wheezing, shortness of breath, eye, nose, and throat irritation, headache, dizziness, and fatigue. Community and individual solutions such as using clean fuel, wearing personal masks, filtering indoor air, and ventilating need to be taken to reduce the impact of air pollution.","PeriodicalId":18650,"journal":{"name":"Ministry of Science and Technology, Vietnam","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2024-03-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140239970","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-03-15DOI: 10.31276/vjste.66(1).111-119
Minh Hieu Vu, Doan Hong Ngoc Tran, Minh-Anh Dang-Trinh, Huynh Chan Khon
The Coronavirus (SARS-CoV-2) is an enveloped RNA virus that caused a dangerous COVID-19 pandemic following an outbreak in Wuhan, China in 2019. In response to the pandemic, the development of lateral flow assays (LFAs) has been crucial for the detection of viruses, commonly targeting the spike (S) or nucleocapsid (N) protein in nasopharyngeal swab (NS) specimens. COVID-19, caused by the SARS-CoV-2 virus, predominantly manifests as a respiratory tract infection-like illness, characterized by symptoms such as fever, dry cough, upper airway congestion, runny nose, sore throat, myalgia, headache, and exhaustion. This study presents the development of an LFA targeting the N protein to detect the coronavirus in saliva specimens, using a sandwich format. The use of 80 nm gold nanoparticles (AuNPs) has been investigated, and the application of horseradish peroxidase (HRP) and TMB substrate has been employed to enhance the limit of detection (LOD). The results demonstrate a significant 200-fold improvement in LOD, from 10 to 50 pg/ml, for N protein spiked in saliva samples after the application of HRP-TMB. This finding highlights an important advancement towards the utilization of saliva samples in diagnostic applications.
{"title":"Development of a lateral flow immunoassay with HRP enhancement for spiked SARS-CoV-2 protein N detection in human saliva","authors":"Minh Hieu Vu, Doan Hong Ngoc Tran, Minh-Anh Dang-Trinh, Huynh Chan Khon","doi":"10.31276/vjste.66(1).111-119","DOIUrl":"https://doi.org/10.31276/vjste.66(1).111-119","url":null,"abstract":"The Coronavirus (SARS-CoV-2) is an enveloped RNA virus that caused a dangerous COVID-19 pandemic following an outbreak in Wuhan, China in 2019. In response to the pandemic, the development of lateral flow assays (LFAs) has been crucial for the detection of viruses, commonly targeting the spike (S) or nucleocapsid (N) protein in nasopharyngeal swab (NS) specimens. COVID-19, caused by the SARS-CoV-2 virus, predominantly manifests as a respiratory tract infection-like illness, characterized by symptoms such as fever, dry cough, upper airway congestion, runny nose, sore throat, myalgia, headache, and exhaustion. This study presents the development of an LFA targeting the N protein to detect the coronavirus in saliva specimens, using a sandwich format. The use of 80 nm gold nanoparticles (AuNPs) has been investigated, and the application of horseradish peroxidase (HRP) and TMB substrate has been employed to enhance the limit of detection (LOD). The results demonstrate a significant 200-fold improvement in LOD, from 10 to 50 pg/ml, for N protein spiked in saliva samples after the application of HRP-TMB. This finding highlights an important advancement towards the utilization of saliva samples in diagnostic applications.","PeriodicalId":18650,"journal":{"name":"Ministry of Science and Technology, Vietnam","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2024-03-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140237977","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-03-15DOI: 10.31276/vjste.66(1).104-110
Chinh Nghia Nguyen, Hang Ngan Dinh, Thu Trang Nguyen, Ha Trang Nguyen, Thi Trang Nguyen, Giang Hoang, Ky Son Chu, Thu Trang Vu
In recent years, cheese consumption in Vietnam is markedly rising, leading to the attention of local milk processors on cheese making. Quark-type cheese, a traditional food in Central Europe, is a type of well-known acid-coagulated cheese product. Milk coagulation properties (MCPs) play an important role to the success of the cheese-making process and are influenced by different parameters. In this study, the quality of raw milk for cheese making and the coagulation conditions in quark-type cheese processing were investigated. Raw milk collected from Phu Dong dairy farm met the requirement of Vietnamese standard for cheese making. The appropriate conditions for coagulation were identified: the heat treatment of milk at 60°C for 15 minutes, coagulation temperature at 40oC and pH 5.5, CaCl2 concentration at 0.04g/l. Under these conditions, a short coagulation time (72.33 seconds) and high curd yields (50.70%) were obtained. These results suggested that quark-type cheese using raw milk in Vietnam could be successfully developed at larger scale.
{"title":"Factors affecting the coagulation of milk protein during quark cheese processing in Vietnam","authors":"Chinh Nghia Nguyen, Hang Ngan Dinh, Thu Trang Nguyen, Ha Trang Nguyen, Thi Trang Nguyen, Giang Hoang, Ky Son Chu, Thu Trang Vu","doi":"10.31276/vjste.66(1).104-110","DOIUrl":"https://doi.org/10.31276/vjste.66(1).104-110","url":null,"abstract":"In recent years, cheese consumption in Vietnam is markedly rising, leading to the attention of local milk processors on cheese making. Quark-type cheese, a traditional food in Central Europe, is a type of well-known acid-coagulated cheese product. Milk coagulation properties (MCPs) play an important role to the success of the cheese-making process and are influenced by different parameters. In this study, the quality of raw milk for cheese making and the coagulation conditions in quark-type cheese processing were investigated. Raw milk collected from Phu Dong dairy farm met the requirement of Vietnamese standard for cheese making. The appropriate conditions for coagulation were identified: the heat treatment of milk at 60°C for 15 minutes, coagulation temperature at 40oC and pH 5.5, CaCl2 concentration at 0.04g/l. Under these conditions, a short coagulation time (72.33 seconds) and high curd yields (50.70%) were obtained. These results suggested that quark-type cheese using raw milk in Vietnam could be successfully developed at larger scale.","PeriodicalId":18650,"journal":{"name":"Ministry of Science and Technology, Vietnam","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2024-03-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140240263","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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