Pub Date : 2024-02-25DOI: 10.31276/vjst.66(2).01-07
Hoa Nguyen, Van Du Vu, Thi Nhu Quynh Nguyen, Le Van Nguyen, Ngoc Anh Do
This study aimed to identify the distribution and analysis of genetic diversity of Chlamydia trachomatisgenotypes in infertile Vietnamese women. A total of 119 endocervical swabs were isolated from infertile women at the National Hospital of Obstetrics and Gynecology, Vietnam, during the period from January 2020 to December 2021, and was diagnosed with Chlamydiatrachomatis infection. 81 sequences of the ompA gene with a size of approximately 1,100 bp were used to determine the Chlamydia trachomatis genotypes by the currently reference sequences from GenBank. Sequence analysis of the ompA gene from the 81 positive patients showed nine genotypes, including E, D/Da, F, G/Ga, J, H, K, B/Ba, and I/Ia. In that, E, D/Da, and F were most prevalent (61.74%). Genotypes E, F, and G/Ga were highly conserved, showing 100% similarity to the alternative reference sequences, whereas ompA sequences of genotypes B/Ba, D/Da, H, I/Ia, J, and K displayed from one to ten nucleotide substitutions. In total, nineteen nucleotide changes were detected, seven of which led to amino-acid substitutions. The findings of the current study showed high prevalence of the genotypes E, D/Da, and F of C. trachomatis in infertile Vietnamese women.
{"title":"Genotype identification and genetic diversity of the ompA gene among Chlamydia trachomatis","authors":"Hoa Nguyen, Van Du Vu, Thi Nhu Quynh Nguyen, Le Van Nguyen, Ngoc Anh Do","doi":"10.31276/vjst.66(2).01-07","DOIUrl":"https://doi.org/10.31276/vjst.66(2).01-07","url":null,"abstract":"This study aimed to identify the distribution and analysis of genetic diversity of Chlamydia trachomatisgenotypes in infertile Vietnamese women. A total of 119 endocervical swabs were isolated from infertile women at the National Hospital of Obstetrics and Gynecology, Vietnam, during the period from January 2020 to December 2021, and was diagnosed with Chlamydiatrachomatis infection. 81 sequences of the ompA gene with a size of approximately 1,100 bp were used to determine the Chlamydia trachomatis genotypes by the currently reference sequences from GenBank. Sequence analysis of the ompA gene from the 81 positive patients showed nine genotypes, including E, D/Da, F, G/Ga, J, H, K, B/Ba, and I/Ia. In that, E, D/Da, and F were most prevalent (61.74%). Genotypes E, F, and G/Ga were highly conserved, showing 100% similarity to the alternative reference sequences, whereas ompA sequences of genotypes B/Ba, D/Da, H, I/Ia, J, and K displayed from one to ten nucleotide substitutions. In total, nineteen nucleotide changes were detected, seven of which led to amino-acid substitutions. The findings of the current study showed high prevalence of the genotypes E, D/Da, and F of C. trachomatis in infertile Vietnamese women.","PeriodicalId":18650,"journal":{"name":"Ministry of Science and Technology, Vietnam","volume":"25 6","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-02-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140432076","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-02-25DOI: 10.31276/vjst.66(2).39-43
Dang Thai Phuong Phan, Thuy Duong Nguyen, Van Ba Vu
In the hybridisation process, pollen grains directly participate in the process through flower pollination. Pollen grain quality determines fruit formation and seed setting. In this study, the pollen grains of the local bitter gourd variety (BD2) and F1 commercial variety were collected at 8 different flowering times (8-11h30) for fertility evaluation in the 2020 Spring-Winter crop season in Ho Chi Minh city. Self-pollinate the 2 varieties over 8-9 hours to evaluate their ability to set seeds. The obtained results indicated that the local variety (BD2) had the highest fertility pollen (61.26%) at 08:30 am and the lowest (46.22%) at 9:00 am. Meanwhile, the F1 commercial variety showed the highest fertility pollen (94.05%) at 11:30 am and the lowest (79.58%) at 8:00 am. The fertility of variety BD2 is 60.21%, resulting in 33.50 seeds per fruit in which the percentage of mature seed is 71.72%. Similarly, the commercial variety found the pollen fertility is 85.55%, resulting in 11.25 seeds per fruit in which the percentage of mature seeds is 84.90%. This information is useful for bitter gourd hybridisation.
{"title":"Pollen fertility at different flowering times and its effect on seed setting of some bitter gourd (Momordica charantia L.) varieties in Ho Chi Minh city","authors":"Dang Thai Phuong Phan, Thuy Duong Nguyen, Van Ba Vu","doi":"10.31276/vjst.66(2).39-43","DOIUrl":"https://doi.org/10.31276/vjst.66(2).39-43","url":null,"abstract":"In the hybridisation process, pollen grains directly participate in the process through flower pollination. Pollen grain quality determines fruit formation and seed setting. In this study, the pollen grains of the local bitter gourd variety (BD2) and F1 commercial variety were collected at 8 different flowering times (8-11h30) for fertility evaluation in the 2020 Spring-Winter crop season in Ho Chi Minh city. Self-pollinate the 2 varieties over 8-9 hours to evaluate their ability to set seeds. The obtained results indicated that the local variety (BD2) had the highest fertility pollen (61.26%) at 08:30 am and the lowest (46.22%) at 9:00 am. Meanwhile, the F1 commercial variety showed the highest fertility pollen (94.05%) at 11:30 am and the lowest (79.58%) at 8:00 am. The fertility of variety BD2 is 60.21%, resulting in 33.50 seeds per fruit in which the percentage of mature seed is 71.72%. Similarly, the commercial variety found the pollen fertility is 85.55%, resulting in 11.25 seeds per fruit in which the percentage of mature seeds is 84.90%. This information is useful for bitter gourd hybridisation.","PeriodicalId":18650,"journal":{"name":"Ministry of Science and Technology, Vietnam","volume":"35 3","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-02-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140432902","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-02-25DOI: 10.31276/vjst.66(2).49-54
The Bien Le, T. Hoang, Thi Nhu Mai Nguyen, Dac Khai Hoang, Manh Cuong Do, Hoai Phong Truong, Quoc-Luan Vu, Van The Vinh Bui, T. Duong
In this study, 4-week-old internodes of in vitroPhyllanthus amarus with 1 cm in length were cut in a half longitudinal section and cultured under 2D clinostat and control conditions to evaluate in vitro morphogenesis, antioxidant enzyme activity and secondary compound accumulation. The results showed that 34.33% of explants induced callus and 65.67% of explants formed adventitious root under 2D clinostat compared with 100% explants induced callus under control after 4 weeks of culture. In addition, the fresh and dry weights of callus clusters (792.00 and 79.17 mg, respectively) under 2D clinostat were higher than those under control (313.33 and 30.07 mg, respectively). Superoxide dismutase (SOD), catalase (CAT), and phenolic under 2D clinostat were higher than those of control. For hypophyllanthin and phyllanthin, the concentrations in the callus under 2D clinostat condition were higher than control. The hypophyllanthin content in the callus (29.06 μg/g fresh weight) was higher than in the adventitious root (9.03 μg/g fresh weight) under 2D clinostat, while the phyllanthin only obtained in the callus that was absent in the adventitious root.
{"title":"In vitro morphogenesis, antioxidant enzyme activity and secondary compound accumulation of Phyllanthus amarus under Clinostat 2D condition","authors":"The Bien Le, T. Hoang, Thi Nhu Mai Nguyen, Dac Khai Hoang, Manh Cuong Do, Hoai Phong Truong, Quoc-Luan Vu, Van The Vinh Bui, T. Duong","doi":"10.31276/vjst.66(2).49-54","DOIUrl":"https://doi.org/10.31276/vjst.66(2).49-54","url":null,"abstract":"In this study, 4-week-old internodes of in vitroPhyllanthus amarus with 1 cm in length were cut in a half longitudinal section and cultured under 2D clinostat and control conditions to evaluate in vitro morphogenesis, antioxidant enzyme activity and secondary compound accumulation. The results showed that 34.33% of explants induced callus and 65.67% of explants formed adventitious root under 2D clinostat compared with 100% explants induced callus under control after 4 weeks of culture. In addition, the fresh and dry weights of callus clusters (792.00 and 79.17 mg, respectively) under 2D clinostat were higher than those under control (313.33 and 30.07 mg, respectively). Superoxide dismutase (SOD), catalase (CAT), and phenolic under 2D clinostat were higher than those of control. For hypophyllanthin and phyllanthin, the concentrations in the callus under 2D clinostat condition were higher than control. The hypophyllanthin content in the callus (29.06 μg/g fresh weight) was higher than in the adventitious root (9.03 μg/g fresh weight) under 2D clinostat, while the phyllanthin only obtained in the callus that was absent in the adventitious root.","PeriodicalId":18650,"journal":{"name":"Ministry of Science and Technology, Vietnam","volume":"28 4","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-02-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140431961","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-02-25DOI: 10.31276/vjst.66(2).32-38
Quang Tri Le, H. Phan, Thi Thanh Tam Vu, Gia Huy Truong, Nguyen Nhu Hieu Tran, Thi Dieu Thuan Hoang, Le Bao Ha Tran, Minh Quan To, Thi Ngoc My Nguyen, Nguyen Vu Doan
Objective: Develop a process and evaluate the effects of local treatment with protein gel on the patient’s skin graft wound. Subjects and methods: The case series study reported the results of treating skin loss wounds with protein gel application on 5 patients at the Department of Orthopedics and Traumatology - 7A Military Hospital from August 2023 to November 2023. Results: At the time of removing the skin graft bandage (day 7), a very good adhesion rate of the skin graft to the graft base was recorded (from 92-97%). After 2 weeks of transplantation, the graft skin adhesion rate reached 97-100%. By 4 weeks after transplantation, the grafted skin adhered 100% to the graft base in all 5 patients and all patients reported good results after skin grafting. Conclusion: The use of protein gel in the skin grafting process increases the adhesion of the grafted skin, reduces pain, reduces exudate at the wound, and increases the survival rate of the grafted skin.
{"title":"Initial evaluation of the application of autologous protein gel in the treatment of extensive skin loss wounds","authors":"Quang Tri Le, H. Phan, Thi Thanh Tam Vu, Gia Huy Truong, Nguyen Nhu Hieu Tran, Thi Dieu Thuan Hoang, Le Bao Ha Tran, Minh Quan To, Thi Ngoc My Nguyen, Nguyen Vu Doan","doi":"10.31276/vjst.66(2).32-38","DOIUrl":"https://doi.org/10.31276/vjst.66(2).32-38","url":null,"abstract":"Objective: Develop a process and evaluate the effects of local treatment with protein gel on the patient’s skin graft wound. Subjects and methods: The case series study reported the results of treating skin loss wounds with protein gel application on 5 patients at the Department of Orthopedics and Traumatology - 7A Military Hospital from August 2023 to November 2023. Results: At the time of removing the skin graft bandage (day 7), a very good adhesion rate of the skin graft to the graft base was recorded (from 92-97%). After 2 weeks of transplantation, the graft skin adhesion rate reached 97-100%. By 4 weeks after transplantation, the grafted skin adhered 100% to the graft base in all 5 patients and all patients reported good results after skin grafting. Conclusion: The use of protein gel in the skin grafting process increases the adhesion of the grafted skin, reduces pain, reduces exudate at the wound, and increases the survival rate of the grafted skin.","PeriodicalId":18650,"journal":{"name":"Ministry of Science and Technology, Vietnam","volume":"26 6","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-02-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140432239","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-02-25DOI: 10.31276/vjst.66(2).14-19
Pisey Chantha, Thanh Khiem Nguyen, Ham Hoi Nguyen, N. H. Nguyen, Hieu Hoc Tran, Tuan Hiep Luong, Van Minh Do, Hai Dang Do, The Duy Nguyen, Hong Quang Pham
Objectives: This study aims to research on the safety and effectiveness of the modified Blumgart pancreaticojejunostomy anastomosis by Satoi in Bach Mai Hospital. Research subjects: Seventy-three patients underwent modified Blumgart pancreaticojejunostomy anastomosis by Satoi after pancreaticoduodenectomy at Gastrointestinal and Hepato-Biliary-Pancreatic Surgery Department, Bach Mai Hospital between October 2020 and April 2023. Method: Cross-sectional study combining retrospective and prospective studies. The Blumgart anastomosis was modified to simplify with 2 U-shaped sutures. Results: The average age of the study subjects was 58.49±12.69 years; the age group over 60 accounted for over 50%; male/female ratio was ~1. The number of patients with complications was 22/73; the total number of complications was 33, including 3 grade-III-or-above complications, according to Clavien-Dindo: 1 case had complications of grade C pancreatic fistula causing late hemorrhage, from the common hepatic artery wall, needs re-surgery, 1 case needs hemostasis under intervention radiology, 1 case needs to be drained under ultrasound guidance, and no patient deaths. Conclusion: The modified Blumgart pancreaticojejunostomy by Satoi is a safe and effective technique.
{"title":"Evaluation of the results of the modified Blumgartpancreatic-intestinal anastomosis technique according to Satoi in pancreaticoduodenectomy at Bach Mai Hospital","authors":"Pisey Chantha, Thanh Khiem Nguyen, Ham Hoi Nguyen, N. H. Nguyen, Hieu Hoc Tran, Tuan Hiep Luong, Van Minh Do, Hai Dang Do, The Duy Nguyen, Hong Quang Pham","doi":"10.31276/vjst.66(2).14-19","DOIUrl":"https://doi.org/10.31276/vjst.66(2).14-19","url":null,"abstract":"Objectives: This study aims to research on the safety and effectiveness of the modified Blumgart pancreaticojejunostomy anastomosis by Satoi in Bach Mai Hospital. Research subjects: Seventy-three patients underwent modified Blumgart pancreaticojejunostomy anastomosis by Satoi after pancreaticoduodenectomy at Gastrointestinal and Hepato-Biliary-Pancreatic Surgery Department, Bach Mai Hospital between October 2020 and April 2023. Method: Cross-sectional study combining retrospective and prospective studies. The Blumgart anastomosis was modified to simplify with 2 U-shaped sutures. Results: The average age of the study subjects was 58.49±12.69 years; the age group over 60 accounted for over 50%; male/female ratio was ~1. The number of patients with complications was 22/73; the total number of complications was 33, including 3 grade-III-or-above complications, according to Clavien-Dindo: 1 case had complications of grade C pancreatic fistula causing late hemorrhage, from the common hepatic artery wall, needs re-surgery, 1 case needs hemostasis under intervention radiology, 1 case needs to be drained under ultrasound guidance, and no patient deaths. Conclusion: The modified Blumgart pancreaticojejunostomy by Satoi is a safe and effective technique.","PeriodicalId":18650,"journal":{"name":"Ministry of Science and Technology, Vietnam","volume":"43 19","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-02-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140432722","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-02-25DOI: 10.31276/vjst.66(2).66-74
Thi Thu Hien Phan, Thi Kim Anh Nguyen, T. Le, Thi Thuy Hang Vu
Gerbera buds are double sterilised, the clean sample rate is up to 90% (ancient yellow gerbera daisies) and 92% (ancient pink gerbera daisies). With the medium supplemented with 1.5 mg/l BAP and 2 mg/l TDZ, the yellow gerbera daisy variety obtained a regeneration rate of 52.17%, the number of regenerated buds was 5 buds/sample. Ancient pink gerbera daisies have a regeneration rate of 53.85%, the number of regenerated buds was 6 buds/sample. When using 1.5 mg/l IBA combined with 2 mg/l BA, the gerbera daisies obtained a bud formation rate of 44.45% with an average number of 5 buds/sample; ancient pink gerbera daisies achieved a high bud formation rate of 46.15%, with an average number of 6 buds/sample. Media with 0.05 mg/l NAA and 0.3 mg/l IBA produced 6-7 roots, about 2.5 cm in length; the temperature of 20-25oC was most suitable for rooting in 2 varieties of gerbera daisy. After forming roots, the plantlets were planted in a substrate consisting of 50% sand + 30% soil + 20% smoked rice husks. The survival rate after 8 weeks and the height of the ancient yellow gerbera daisies and ancient pink gerbera daisies reached 98.26%, 14.8 cm, 99.05%, and 12.6 cm, respectively.
{"title":"In vitro rapid multiplication of two ancient gerbera varieties (Gerbera jamesonii Bolus)","authors":"Thi Thu Hien Phan, Thi Kim Anh Nguyen, T. Le, Thi Thuy Hang Vu","doi":"10.31276/vjst.66(2).66-74","DOIUrl":"https://doi.org/10.31276/vjst.66(2).66-74","url":null,"abstract":"Gerbera buds are double sterilised, the clean sample rate is up to 90% (ancient yellow gerbera daisies) and 92% (ancient pink gerbera daisies). With the medium supplemented with 1.5 mg/l BAP and 2 mg/l TDZ, the yellow gerbera daisy variety obtained a regeneration rate of 52.17%, the number of regenerated buds was 5 buds/sample. Ancient pink gerbera daisies have a regeneration rate of 53.85%, the number of regenerated buds was 6 buds/sample. When using 1.5 mg/l IBA combined with 2 mg/l BA, the gerbera daisies obtained a bud formation rate of 44.45% with an average number of 5 buds/sample; ancient pink gerbera daisies achieved a high bud formation rate of 46.15%, with an average number of 6 buds/sample. Media with 0.05 mg/l NAA and 0.3 mg/l IBA produced 6-7 roots, about 2.5 cm in length; the temperature of 20-25oC was most suitable for rooting in 2 varieties of gerbera daisy. After forming roots, the plantlets were planted in a substrate consisting of 50% sand + 30% soil + 20% smoked rice husks. The survival rate after 8 weeks and the height of the ancient yellow gerbera daisies and ancient pink gerbera daisies reached 98.26%, 14.8 cm, 99.05%, and 12.6 cm, respectively.","PeriodicalId":18650,"journal":{"name":"Ministry of Science and Technology, Vietnam","volume":"19 10","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-02-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140433180","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-01-25DOI: 10.31276/vjst.66(1).68-74
Thi Ngoc Lan Nguyen, Hoang Bich Nga Le, Thanh Huyen Tran, Thi Thuy Quynh Tran
Blood glucose testing, including capillary blood glucose, is the recommended test for the diagnosis and monitoring of diabetes treatment. Currently, capillary blood glucose testing is performed on point-of-care testing (POCT) with reasonable cost, fast time, convenience, supporting timely diagnosis and treatment. However, in order to select POCT devices that meet quality standards, it is necessary to strictly control the laboratory. Object and method: Confirmation of the capillary blood sugar testing method on the Accu-chek Inform II (Acc-II) machine including the following indicators: accuracy, precision, linear range, comparison of test results according to the recommendations of the Clinical & Laboratory Standards Institute (CLSI) (EP15A3, EP06A, EP09A3). The authors compared results on the testing machine in the central laboratory (Cobas 8000) of blood glucose testing on the POCT machine with the results of whole capillary blood, whole venous blood, and plasma venous samples. Results: Precision, accuracy, and linearity were confirmed as published. The linear range of the method is from 1.6 to 31 mmol/l. The difference between capillary blood glucose pairs and plasma venous glucose, whole blood venous glucose on Accu-chek Inform II and plasma venous blood glucose on Cobas 8000 machine are 6.89, -8.62, and -10.97%, respectively.
{"title":"Validation of the blood glucose testing method on the Accu-chek Inform II","authors":"Thi Ngoc Lan Nguyen, Hoang Bich Nga Le, Thanh Huyen Tran, Thi Thuy Quynh Tran","doi":"10.31276/vjst.66(1).68-74","DOIUrl":"https://doi.org/10.31276/vjst.66(1).68-74","url":null,"abstract":"Blood glucose testing, including capillary blood glucose, is the recommended test for the diagnosis and monitoring of diabetes treatment. Currently, capillary blood glucose testing is performed on point-of-care testing (POCT) with reasonable cost, fast time, convenience, supporting timely diagnosis and treatment. However, in order to select POCT devices that meet quality standards, it is necessary to strictly control the laboratory. Object and method: Confirmation of the capillary blood sugar testing method on the Accu-chek Inform II (Acc-II) machine including the following indicators: accuracy, precision, linear range, comparison of test results according to the recommendations of the Clinical & Laboratory Standards Institute (CLSI) (EP15A3, EP06A, EP09A3). The authors compared results on the testing machine in the central laboratory (Cobas 8000) of blood glucose testing on the POCT machine with the results of whole capillary blood, whole venous blood, and plasma venous samples. Results: Precision, accuracy, and linearity were confirmed as published. The linear range of the method is from 1.6 to 31 mmol/l. The difference between capillary blood glucose pairs and plasma venous glucose, whole blood venous glucose on Accu-chek Inform II and plasma venous blood glucose on Cobas 8000 machine are 6.89, -8.62, and -10.97%, respectively.","PeriodicalId":18650,"journal":{"name":"Ministry of Science and Technology, Vietnam","volume":"123 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-01-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140495497","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-01-25DOI: 10.31276/vjst.66(1).50-55
V. V. Nguyen, Thi Hang Pham, D. P. Huynh
Cotton fabric is commonly used in the textile industry owing to good ventilation and absorption capacity. However, dirt resistance and water resistance of cotton fabrics are low. Therefore, coating cotton fabrics with water-repellent polysiloxane is a special requirement for outdoor and protective clothing products. The effects of polysiloxane content on the water resistance and mechanical properties of materials were studied. The morphology of coated cotton fabric was characterised by SEM (Scanning electron microscope), and EDX (Energy dispersive X-ray) was used to determine the presence of polysiloxane on the surface of cotton fabric. The effects of polysiloxane content on the water resistance and mechanical properties of materials were studied. Compared with raw cotton fabrics, the tensile strength of the cotton fabrics impregnated with 20%wt polysiloxane solution increased, but the elongation at break was reduced. The hydrostatic pressure (AATCC 127) and water spray test (AATCC 22) results showed that the water repellence of polysiloxane-coated cotton fabrics increased significantly compared to pure cotton fabrics. In addition, the polysiloxane content has no almost effect on the water repellency of the material. Therefore, the appropriate use of polysiloxane can contribute to minimising the cost of the product.
棉织物具有良好的透气性和吸湿性，因此常用于纺织业。然而，棉织物的抗污性和防水性较低。因此，在棉织物上涂覆防水聚硅氧烷是户外和防护服产品的特殊要求。研究了聚硅氧烷含量对材料耐水性和机械性能的影响。利用 SEM（扫描电子显微镜）和 EDX（能量色散 X 射线）对涂层棉织物的形态进行了表征，以确定棉织物表面是否存在聚硅氧烷。研究了聚硅氧烷含量对材料耐水性和机械性能的影响。与原棉织物相比，浸渍了 20%wt 聚硅氧烷溶液的棉织物的拉伸强度提高了，但断裂伸长率降低了。静水压试验（AATCC 127）和水喷雾试验（AATCC 22）结果表明，与纯棉织物相比，聚硅氧烷涂层棉织物的拒水性显著提高。此外，聚硅氧烷含量对材料的拒水性几乎没有影响。因此，适当使用聚硅氧烷有助于最大限度地降低产品成本。
{"title":"The influence of polysiloxane on the mechanical properties and water repellency of cotton fabrics","authors":"V. V. Nguyen, Thi Hang Pham, D. P. Huynh","doi":"10.31276/vjst.66(1).50-55","DOIUrl":"https://doi.org/10.31276/vjst.66(1).50-55","url":null,"abstract":"Cotton fabric is commonly used in the textile industry owing to good ventilation and absorption capacity. However, dirt resistance and water resistance of cotton fabrics are low. Therefore, coating cotton fabrics with water-repellent polysiloxane is a special requirement for outdoor and protective clothing products. The effects of polysiloxane content on the water resistance and mechanical properties of materials were studied. The morphology of coated cotton fabric was characterised by SEM (Scanning electron microscope), and EDX (Energy dispersive X-ray) was used to determine the presence of polysiloxane on the surface of cotton fabric. The effects of polysiloxane content on the water resistance and mechanical properties of materials were studied. Compared with raw cotton fabrics, the tensile strength of the cotton fabrics impregnated with 20%wt polysiloxane solution increased, but the elongation at break was reduced. The hydrostatic pressure (AATCC 127) and water spray test (AATCC 22) results showed that the water repellence of polysiloxane-coated cotton fabrics increased significantly compared to pure cotton fabrics. In addition, the polysiloxane content has no almost effect on the water repellency of the material. Therefore, the appropriate use of polysiloxane can contribute to minimising the cost of the product.","PeriodicalId":18650,"journal":{"name":"Ministry of Science and Technology, Vietnam","volume":"11 4","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-01-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140495092","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-01-25DOI: 10.31276/vjst.66(1).24-28
Tien Dung Nguyen, Thi Thu Ha Tran, Duc-Huy Nguyen, Ngoc-Ai Trinh, Phi Hung Nguyen
Nowadays, the demand for using herb plants to treat and protect human health is increasing. Along with the growing herbal market, the adulteration of herbal medicinal materials is increasing in many countries, including Vietnam. It is difficult to determine the origin of herbal medicinal materials by morphological or chemical methods when they are substituted by herbs from closely related species or adulterated intentionally by materials from unrelated plants or processed materials. Therefore, it is necessary to use molecular methods based on DNA markers. ITS is a highly conservative nuclear ribosomal marker that has been widely used in the identification of plant species. In this study, the ITS2 marker was used to identify 8 herbal medicinal materials, including 2 samples of polyscias(DLTN, DLBG), 2 samples of morinda (BKTN, BKQN), 2 samples of black zingiber (GDTN, GDYB) and 2 samples of black curcuma (NDTN, NDYB). Gene analysis and sequencing results showed that the DLTN and DLBG were Polyscias fruticosa; BKTN and BKQN were different species, Gynochthodes officinalis (BKTN) and Morinda officinalis (BKQN); GDTN and GDYB were Kaempferia parviflora; NDTN and NDYB were Curcuma caesia. The research results show that the ITS2 indicator can be used to identify medicinal species.
{"title":"Application of DNA barcode ITS2to identify herbal medicinal materials","authors":"Tien Dung Nguyen, Thi Thu Ha Tran, Duc-Huy Nguyen, Ngoc-Ai Trinh, Phi Hung Nguyen","doi":"10.31276/vjst.66(1).24-28","DOIUrl":"https://doi.org/10.31276/vjst.66(1).24-28","url":null,"abstract":"Nowadays, the demand for using herb plants to treat and protect human health is increasing. Along with the growing herbal market, the adulteration of herbal medicinal materials is increasing in many countries, including Vietnam. It is difficult to determine the origin of herbal medicinal materials by morphological or chemical methods when they are substituted by herbs from closely related species or adulterated intentionally by materials from unrelated plants or processed materials. Therefore, it is necessary to use molecular methods based on DNA markers. ITS is a highly conservative nuclear ribosomal marker that has been widely used in the identification of plant species. In this study, the ITS2 marker was used to identify 8 herbal medicinal materials, including 2 samples of polyscias(DLTN, DLBG), 2 samples of morinda (BKTN, BKQN), 2 samples of black zingiber (GDTN, GDYB) and 2 samples of black curcuma (NDTN, NDYB). Gene analysis and sequencing results showed that the DLTN and DLBG were Polyscias fruticosa; BKTN and BKQN were different species, Gynochthodes officinalis (BKTN) and Morinda officinalis (BKQN); GDTN and GDYB were Kaempferia parviflora; NDTN and NDYB were Curcuma caesia. The research results show that the ITS2 indicator can be used to identify medicinal species.","PeriodicalId":18650,"journal":{"name":"Ministry of Science and Technology, Vietnam","volume":"118 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-01-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140495456","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-01-25DOI: 10.31276/vjst.66(1).08-13
Thi Lan Anh Nguyen, Thi Thu Hien Tran, Thi Phuong Pham, Thi Thu Hien Tran, Hai Yen Dao
Organophosphate flame retardants (OPFRs) are degraded by a consortium of 10 bacterial strains isolated from the To Lich river, contaminated with OPFRs. The bacterial consortium is cultured in an A-Cl medium supplemented individually with OPFRs (10 mg/l), resulting in optical density at 600 nm ranging from 1 to 1.5. The consortium of 10 bacterial strains exhibits the ability to degrade tris(2-ethylhexyl) phosphate (TEHP) by 98.5 and 100% after two and three days of cultivation, respectively. Triethyl phosphate (TEP) is also degraded by 76.1 and 100% after two and five days of cultivation, respectively. The degradation efficiency of trimethyl phosphate (TMP), tris(2-chloroethyl) phosphate (TCEP), tris(1,3-dichloro-2-propyl) phosphate (TCDPP), and tributoxy ethyl phosphate (TBEP) is also in the range of 97 to 100% after four days of cultivation. In the experiment adding a mixture of 7 substances in OPFRs at a concentration of 10 mg/l for each compound, only TEHP is completely degraded after six days of cultivation. The degradation efficiency of other OPFRs increases from 93.2 to 100% after six days of cultivation. Comparing the degradation rates of OPFRs in both experiments, the results indicate that the cultures supplemented with each compound individually exhibit a faster degradation rate than when a mixture of OPFRs compounds is added.
{"title":"Degradation of organophosphate flame retardants by a consortium of bacterial strains isolated from the To Lich river, Hanoi","authors":"Thi Lan Anh Nguyen, Thi Thu Hien Tran, Thi Phuong Pham, Thi Thu Hien Tran, Hai Yen Dao","doi":"10.31276/vjst.66(1).08-13","DOIUrl":"https://doi.org/10.31276/vjst.66(1).08-13","url":null,"abstract":"Organophosphate flame retardants (OPFRs) are degraded by a consortium of 10 bacterial strains isolated from the To Lich river, contaminated with OPFRs. The bacterial consortium is cultured in an A-Cl medium supplemented individually with OPFRs (10 mg/l), resulting in optical density at 600 nm ranging from 1 to 1.5. The consortium of 10 bacterial strains exhibits the ability to degrade tris(2-ethylhexyl) phosphate (TEHP) by 98.5 and 100% after two and three days of cultivation, respectively. Triethyl phosphate (TEP) is also degraded by 76.1 and 100% after two and five days of cultivation, respectively. The degradation efficiency of trimethyl phosphate (TMP), tris(2-chloroethyl) phosphate (TCEP), tris(1,3-dichloro-2-propyl) phosphate (TCDPP), and tributoxy ethyl phosphate (TBEP) is also in the range of 97 to 100% after four days of cultivation. In the experiment adding a mixture of 7 substances in OPFRs at a concentration of 10 mg/l for each compound, only TEHP is completely degraded after six days of cultivation. The degradation efficiency of other OPFRs increases from 93.2 to 100% after six days of cultivation. Comparing the degradation rates of OPFRs in both experiments, the results indicate that the cultures supplemented with each compound individually exhibit a faster degradation rate than when a mixture of OPFRs compounds is added.","PeriodicalId":18650,"journal":{"name":"Ministry of Science and Technology, Vietnam","volume":"37 2","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-01-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140495013","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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