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In vitro propagation of Philodendron hederaceum “Lemon Lime” 试管繁殖 Philodendron hederaceum "Lemon Lime" (柠檬柠条
Pub Date : 2023-12-25 DOI: 10.31276/vjst.65(12).64-69
Thi Thao Ninh, Hong Thuong Nguyen, Truong Son Dinh, Thi Dung Pham, Thi Hue Nong
This study aims to examine the in vitro propagation of Philodendron hederaceum “Lemon Lime”. The results showed that MS medium supplemented with 1.0 mg/l BA was suitable for the rapid multiplication stage, with the rate of shoot proliferation being 2.73 times, the shoots being 5.38 cm in height and the number of leaves/shoot was 4.33 after 6 weeks of culture. In the next stage, in vitro shoots were transferred to MS medium containing 0.25 mg/l IBA for rooting. Within five weeks of culture, 100% of shoots induced roots with a mean of 3.9 roots/plant and the root length was 11.04 cm. In the acclimatisation phase, plantlets achieved 100% ex vitro survival, increased by 2.19 cm in height and 1.96 leaves after four-week acclimatisation in a substrate composed of peatmoss:soil:coconut husk (1:1:1). These research results could be used to cultivate this plant with high proliferation rate and good commercial quality providing seedlings for the floriculture market in Vietnam.
本研究旨在考察 "柠檬莱姆"(Philodendron hederaceum "Lemon Lime")的离体繁殖。结果表明,添加 1.0 毫克/升 BA 的 MS 培养基适用于快速繁殖阶段,培养 6 周后,芽的增殖率为 2.73 倍,芽的高度为 5.38 厘米,叶片数/芽为 4.33。下一阶段,将离体芽转移到含 0.25 毫克/升 IBA 的 MS 培养基中进行生根。在 5 周的培养过程中,100% 的芽都生出了根,平均每株生根 3.9 根,根长 11.04 厘米。在适应阶段,在泥炭藓:土壤:椰子壳(1:1:1)组成的基质中适应四周后,小植株的离体存活率达到 100%,高度增加了 2.19 厘米,叶片增加了 1.96 片。这些研究成果可用于培育这种具有高增殖率和良好商业品质的植物,为越南花卉市场提供种苗。
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引用次数: 0
Results of laparoscopic Heller-Toupet operation for achalasia 腹腔镜海勒-图佩特手术治疗贲门失弛缓症的效果
Pub Date : 2023-12-25 DOI: 10.31276/vjst.65(12).15-18
Dang Hung Nguyen, Duc Huan Pham, Hoang Nguyen
Objectives: This study was carried out to evaluate surgical outcomes of laparoscopic Heller-Toupet surgery for achalasia. Methodology: This is a retrospective case series implemented at the Gastrointestinal Department, Hanoi Medical University Hospital between January 2015 and August 2022, including 71 patients with achalasia who underwent laparoscopic Heller-Toupet. Results: 52.1% of patients in the study are male (more than female 47.9%). The average age of the cohort was 44.9±13.9 years. The incidence of dysphagia was 100%. Most patients report esophageal dilatation grade II (76.1%) and disease severity stage I (57.7%). The mean muscle opening length was 7.4±1.3 cm, the operative time was 135±25 minutes. There was no conversion, no intra-and postoperative adverse events. Long-term outcomes: good (no or very mild dysphagia) was 90.1%, moderate (moderate dysphagia) was 9.9% and clinical incidence of reflux syndrome was 17%. There was no case requiring intervention or re-operation. Conclusions: Laparoscopic Heller-Toupet is a safe, low rate of intra- and postoperative complication, and brings good long-term outcomes in the treatment of achalasia.
研究目的本研究旨在评估腹腔镜 Heller-Toupet 手术治疗贲门失弛缓症的效果。研究方法:这是一项回顾性病例系列研究,于2015年1月至2022年8月期间在河内医科大学附属医院胃肠科实施,包括71名接受腹腔镜Heller-Toupet手术的贲门失弛缓症患者。研究结果52.1%的患者为男性(47.9%多于女性)。平均年龄为(44.9±13.9)岁。吞咽困难发生率为 100%。大多数患者的食管扩张程度为 II 级(76.1%),疾病严重程度为 I 级(57.7%)。平均肌肉开口长度为(7.4±1.3)厘米,手术时间为(135±25)分钟。无转归,术中术后无不良反应。长期疗效:良好(无或极轻度吞咽困难)占90.1%,中度(中度吞咽困难)占9.9%,反流综合征的临床发生率为17%。没有需要干预或再次手术的病例。结论腹腔镜海勒-图佩特术是一种安全、术中和术后并发症发生率低、长期疗效好的贲门失弛缓症治疗方法。
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引用次数: 0
Clinical and paraclinical characteristics of acute bronchitis patients at Hanoi Hospital of Traditional Medical 河内传统医学医院急性支气管炎患者的临床和辅助临床特征
Pub Date : 2023-12-25 DOI: 10.31276/vjst.65(12).19-22
Thi Ngoan Vu, Quynh Hoa Ngo
Objectives: To describe the clinical and paraclinical features of inpatients with acute bronchitis treated at Hanoi Hospital of Traditional Medical. Subjects and methods: A cross-sectional study was conducted on 60 acute bronchitis patients from April to December 2021. Eligible patients included in the study were carried out with clinical examination, blood test, and chest X-ray. Results: The mean age in the study was 66.78±18.33 years old; the patients over 70 years old are 56.67%; the female/male ratio is 1/1.03; 21.70% of patients had smoking tobacco and pipe tobacco. Cough, thick sticky yellow sputum difficult to expectorate, and crackles were common symptoms in all patients in this study. Fever, dyspnea, and chest pain while coughing accounted for 63.30, 28.33, and 46.67%. Most of the patients had a red tongue, yellow tongue moss, and float fast pulse. Peripheral blood tests showed that increased amount of leukocytes, mainly in neutrophils. Erythrocyte sedimentation rate and C-reactive protein (CRP) increased with mean values of 36.33±15.47 mm and 25.49±16.08 mg/l.
目的描述河内传统医学医院收治的急性支气管炎住院患者的临床和辅助临床特征。研究对象和方法:2021 年 4 月至 12 月期间,对 60 名急性支气管炎患者进行了横断面研究。对符合条件的患者进行了临床检查、血液检测和胸部 X 光检查。研究结果平均年龄为(66.78±18.33)岁;70 岁以上患者占 56.67%;男女比例为 1/1.03;21.70%的患者吸烟及烟斗。咳嗽、难以排出的黄色粘稠痰液和噼啪声是所有患者的常见症状。发热、呼吸困难和咳嗽时胸痛分别占 63.30%、28.33% 和 46.67%。大多数患者舌质红,舌苔黄,脉浮快。外周血检查显示白细胞增多,以中性粒细胞为主。红细胞沉降率和 C 反应蛋白(CRP)升高,平均值分别为(36.33±15.47)毫米和(25.49±16.08)毫克/升。
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引用次数: 0
Clinical features, paraclinical characteristics, and etiology of mediastinal bleeding diffusion in children at the Vietnam National Children’s Hospital from 2018 to 2023 2018年至2023年越南国家儿童医院儿童纵隔出血弥漫的临床特征、副临床特征和病因
Pub Date : 2023-12-25 DOI: 10.31276/vjst.65(12).10-14
Thi Le Nguyen, Thi Phuong Thanh Hoang, Thi Thu Hang Hoang, Tuan Hung Phan
Background: In modern life, diffuse alveolar haemorrhage (DAH) is a serious and rare condition in children. Due to the subtle clinical manifestations and diverse causes of the disease, it is easy to be missed or diagnosed late. This study describes clinical and paraclinical characteristics, with the aim to find signs suggesting early diagnosis as well as determine the cause of DAH in children. Objects and methods: Case series. Results: From January 2018 to March 2023, there were 14 children diagnosed with DAH. The age of onset is 4.08 (1.58-5.41) years old, but most patients are diagnosed late with age at diagnosis being 5.25 (1.67-6.16) years old, male/female ratio is 1:1.8. The most common symptoms reported were anaemia (100%), hemoptysis (57.1%), shortness of breath (71.4%), and respiratory failure (85.7%). The common X-ray finding was diffuse infiltrates in both lungs (92.9%). On chest computed tomography scans, 92.9% of patients exhibited images of blurred opacity spreading, 57.1% showed interlobar pleural thickening, and 28.6% presented with images of lung consolidation. 3 patients (22%) had milk allergy, 2 patients (14%) had ANCA (Anti-neutrophil cytoplasmic antibody) vasculitis, and 9 patients (64%) had idiopathic pulmonary haemosiderosis (IPH).
背景:在现代生活中,弥漫性肺泡出血(DAH)是一种严重而罕见的儿童疾病。由于该病临床表现隐匿,病因多样,很容易被漏诊或晚诊。本研究描述了该病的临床和辅助临床特征,旨在发现提示早期诊断的体征,并确定儿童 DAH 的病因。对象和方法:病例系列。结果:自2018年1月至2023年3月,共有14名儿童确诊为DAH。发病年龄为 4.08(1.58-5.41)岁,但大多数患者确诊较晚,确诊年龄为 5.25(1.67-6.16)岁,男女比例为 1:1.8。最常见的症状是贫血(100%)、咯血(57.1%)、气短(71.4%)和呼吸衰竭(85.7%)。常见的 X 光检查结果是双肺弥漫性浸润(92.9%)。在胸部计算机断层扫描中,92.9%的患者表现为模糊的翳扩散图像,57.1%的患者表现为叶间胸膜增厚,28.6%的患者表现为肺部合并症图像。3名患者(22%)对牛奶过敏,2名患者(14%)患有ANCA(抗中性粒细胞胞浆抗体)血管炎,9名患者(64%)患有特发性肺血肿(IPH)。
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引用次数: 0
Fabrication and evaluation of some characteristics of the skin's dermis gel direction to application in regenerative medicine 制作和评估皮肤真皮层凝胶的一些特征,以便应用于再生医学
Pub Date : 2023-12-25 DOI: 10.31276/vjst.65(12).34-37
Le Bao Ha Tran, Nguyen Vu Doan, Thi Ngoc My Nguyen, Minh Quan To, Thi Vy Tuyet Le, Thuan Nho Nguyen, Thanh Vy Truong, Thi Hong Tham Nguyen, Thi Thanh Tam Vu, H. Phan, Quang Tri Le
Objective:The tight connection between the cell-extracellular matrix (ECM - Extracellular matrix) is the foundation of tissue development and repair. The extracellular framework simulates the biological properties of organism-based ECM. The ECM of the skin’s dermis is a natural material, containing ingredients ideal for creating products that help tissue heal. This study focuses on the production of gel products from human skin ECM. The skin ECM is gelled by dissolving in a pepsin enzyme solution.Method: 3D gel formation process using neutral enzyme solution and incubation under physiological conditions. Gel samples were evaluated for speed and formability. Additionally, the dermal gel preparation was evaluated for its effects on the survival and proliferation of human fibroblasts in vitro by toxicity testing and proliferation testing, for 10 days. Results: Direct and extract toxicity assessments showed that the ECM gel regimen was safe for fibroblast survival. At the same time, fibroblasts had proliferation properties after 10 days in culture conditions with gel extract.Conclusion: These results showed that the ECM gel product regimen has the ability to positively impact the biological properties of cells and has the potential for application in the field of regenerative medicine.
目的:细胞-细胞外基质(ECM - Extracellular matrix)之间的紧密联系是组织发育和修复的基础。细胞外基质框架模拟了生物体细胞外基质的生物特性。皮肤真皮层的 ECM 是一种天然材料,其中的成分非常适合用于制造帮助组织愈合的产品。本研究的重点是利用人体皮肤 ECM 生产凝胶产品。皮肤 ECM 在胃蛋白酶溶液中溶解后形成凝胶:方法:使用中性酶溶液进行三维凝胶形成过程,并在生理条件下进行培养。对凝胶样品的速度和成型性进行了评估。此外,还通过毒性测试和增殖测试对真皮凝胶制剂对人成纤维细胞体外存活和增殖的影响进行了为期 10 天的评估。结果显示直接和提取物毒性评估表明,ECM 凝胶疗法对成纤维细胞的存活是安全的。同时,在凝胶提取物的培养条件下,成纤维细胞在 10 天后具有增殖特性:这些结果表明,ECM 凝胶产品方案能够对细胞的生物特性产生积极影响,具有在再生医学领域应用的潜力。
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引用次数: 0
Changes in basic water environmental parameters of the water intake of brackish water shrimp farming in Northern Vietnam period 2017-2021 2017-2021 年越南北部咸水虾养殖取水口基本水环境参数的变化
Pub Date : 2023-12-25 DOI: 10.31276/vjst.65(12).43-51
H. N. Nguyen, Trong Binh Phan, Thi Minh Nguyet Nguyen, Duc-Binh Nguyen, T. Pham, Thi Thanh Pham
This study synthesised and analysed water environmental parameters data of water supply of brackish water shrimp farming in Northern Vietnam over 5 years from 2017 to 2021 to find out the changing patterns of the parameters, thereby making appropriate recommendations for stakeholders. There were 7 analysed and evaluated water environment parameters, including water temperature, dissolved oxygen (DO), salinity, pH, alkalinity, chemical oxygen demand (COD), nitrite (N-NO2), with monitoring frequency of 2 times/month, 10 months/year at 13 monitoring locations for brackish water shrimp farming in 6 provinces: Nam Dinh, Nghe An, Ha Tinh, Quang Binh, Quang Tri, and Thua Thien Hue. Water temperature was low at the beginning of the year, gradually increased to the highest level in June, then gradually decreased to the end of the year. The temperature fluctuated strongly at the beginning and end of the year and tended to increase gradually from the North to the South. DO tended to decrease from the beginning of the year to the end of the year. Salinity was lower in the rainy season, especially in Nghe An and Quang Tri, compared to the others. The pH and alkalinity levels were suitable for brackish shrimp farming. COD in Ha Tinh and Nam Dinh, NO2 in Nam Dinh were often high, indicating that the supply water was contaminated with organic matter and needed a long-term solution.
本研究综合分析了2017年至2021年5年间越南北部咸淡水养虾供水的水环境参数数据,以找出参数的变化规律,从而为利益相关者提出适当的建议。共分析和评估了 7 项水环境参数,包括水温、溶解氧 (DO)、盐度、pH 值、碱度、化学需氧量 (COD)、亚硝酸盐 (N-NO2),监测频率为每月 2 次,每年 10 个月,在 6 个省的 13 个咸水虾养殖监测点进行:在南定、义安、河静、广平、广治省和顺化省的 13 个咸水虾养殖监测点进行了监测,监测频率为每月 2 次,每年 10 个月。年初水温较低,6 月份逐渐升至最高,然后逐渐下降至年底。温度在年初和年末波动较大,并有从北向南逐渐升高的趋势。溶解氧从年初到年末呈下降趋势。与其他地区相比,雨季的盐度较低,尤其是义安省和广治省。pH 值和碱度适合咸水养虾。河静省和南定省的化学需氧量、南定省的二氧化氮通常较高,表明供水受到有机物污染,需要长期解决。
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引用次数: 0
Research of newborn screening of Glucose-6-phosphate dehydrogenase (G6PD) deficiency at Medlatec General Hospital Medlatec 综合医院对新生儿葡萄糖-6-磷酸脱氢酶(G6PD)缺乏症筛查的研究
Pub Date : 2023-12-25 DOI: 10.31276/vjst.65(12).06-09
Thi Lan Anh Ta, Thi Que Trinh, T. Vu, Thi Thu Hien Dao, Thi Ngoc Lan Hoang, Thi Kim Phuong Doan
Objective: Survey some factors affecting Glucose-6-phosphate dehydrogenase (G6PD) enzyme activity from newborn heel blood samples and analyse screening results of some cases of G6PD enzyme deficiency. Research method: Retrospective and prospective cross-sectional description combined with case description. Results: Research on 3223 children who performed the newborn heel blood screening test from September 2022 to September 2023 showed that the high-risk rate of G6PD enzyme deficiency in boys is significantly higher than in girls. The difference is statistically significant when comparing the proportion of neonates with high-risk screening results in the Muong ethnic group and other ethnic minorities compared to the Kinh ethnic group (p<0.01). Between different sampling age groups and different weight groups, the proportion of children with enzyme activity at the high-risk threshold for G6PD enzyme deficiency did not have a statistically significant difference (p>0.05). The mutation encountered in the study was G6PD Union (c.1360C>T) (p.Arg454Cys), the enzyme activity decreased by <10% corresponding to the level of class II enzyme deficiency, showing mild hemolysis and no severe consequences in infancy. Conclusion: Children's gender and ethnicity are related to enzyme activity at risk thresholds for G6PD enzyme deficiency. Studying the correlation between genotype and phenotype is of great significance in genetic counselling, predicting prognosis and managing G6PD enzyme deficiency.
目的调查影响新生儿足跟血样本中葡萄糖-6-磷酸脱氢酶(G6PD)酶活性的一些因素,并分析一些 G6PD 酶缺乏症病例的筛查结果。研究方法:回顾性和前瞻性横断面描述与病例描述相结合。研究结果:对2022年9月至2023年9月进行新生儿足跟血筛查的3223名儿童的研究表明,男孩G6PD酶缺乏症的高风险率明显高于女孩。与京族相比,苗族和其他少数民族的新生儿高危筛查结果比例差异有统计学意义(P0.05)。研究中发现的基因突变为 G6PD 联盟(c.1360C>T)(p.Arg454Cys),酶活性降低<10%,相当于 II 类酶缺乏水平,在婴儿期表现为轻度溶血,无严重后果。结论儿童的性别和种族与 G6PD 酶缺乏症风险阈值的酶活性有关。研究基因型和表型之间的相关性对遗传咨询、预测预后和管理 G6PD 酶缺乏症具有重要意义。
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引用次数: 0
Study on rapid multiplication of two hybrid Arabica coffee varieties F1 Centroamericano (H1) and Mundo Maya (H16) through somatic embryogenesis and Bioreactor technology 通过体细胞胚胎发生和生物反应器技术快速繁殖两个阿拉比卡杂交咖啡品种 F1 Centroamericano (H1) 和 Mundo Maya (H16) 的研究
Pub Date : 2023-12-25 DOI: 10.31276/vjst.65(12).56-63
N. G. Khong, Thi Nhu Le
In this paper, the research focuses on determining some components and culture conditions in order to optimise the 4 main steps of somatic embryogenesis and embryo regeneration by Bioreactor of two Arabica F1 coffee varieties H1 and H16, including: (1) formation of embryogenic callus, (2) multiplication of embryogenic cells, (3) embryonic differentiation, and (4) regeneration of somatic embryo using the temporary immersion Bioreactor RITA 1L. The results showed that on the medium supplemented with Kinetin 2 mg/ll and Phytagel 4 g/l, the callus formation rate reached 94.2 and 92.1% for 2 varieties H1 and H16, respectively. Besides, the addition of Glycine 20 mg/l to the culture medium increased the embryogenic callus rate by 58.1% in both varieties. Adding Trichostatin A 0.03 mg/l in the embryo differentiation medium in the first 7 days of culture showed that the number of torpedo embryos was scored from 1g of initial callus up to 1287 in H1 and 1635 in H16. Finally, using Bioreactor 1L-RITA, soaking for 1 min every 6 hours, the regeneration efficiency reached 75.02% in H1 and 82.03% in H16.
本文的研究重点是确定一些成分和培养条件,以优化使用生物反应器对两个阿拉比卡 F1 咖啡品种 H1 和 H16 进行体细胞胚胎发生和胚胎再生的 4 个主要步骤,包括:(1) 胚胎发生胼胝体的形成;(2) 胚胎发生细胞的繁殖;(3) 胚胎分化;(4) 使用临时浸泡生物反应器 RITA 1L 进行体细胞胚胎再生。结果表明,在添加了 Kinetin 2 mg/ll 和 Phytagel 4 g/l 的培养基上,H1 和 H16 两个品种的胼胝体形成率分别达到 94.2%和 92.1%。此外,在培养基中添加甘氨酸 20 毫克/升,两个品种的胚性胼胝体形成率均提高了 58.1%。在胚分化培养基中添加 0.03 毫克/升的 Trichostatin A,在培养的前 7 天,鱼雷胚的数量从 1 克初始胼胝体增加到 H1 的 1287 个和 H16 的 1635 个。最后,使用生物反应器 1L-RITA,每 6 小时浸泡 1 分钟,H1 的再生效率达到 75.02%,H16 的再生效率达到 82.03%。
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引用次数: 0
Determination of the composition of hookworm species (Ancylostoma duodenale, Ancylostoma ceylanicum)and Necator americanus in humans at community in Tay Ninh province by biomolecular technique 利用生物分子技术确定西宁省社区中人体内钩虫种类(十二指肠肛门疽、塞兰肛门疽)和美洲钩虫的组成成分
Pub Date : 2023-12-25 DOI: 10.31276/vjst.65(12).23-27
Duc Vinh Le, Trinh Vuong Tran, Kim Thach Nguyen
Hookworm infections are common in Vietnam and are caused by two known pathogens: Necator americanus and Ancylostoma duodenale. Recent studies have identified a new hookworm species - Ancylostoma ceylanicum, which typically parasitises in dogs. To determine the prevalence of these three hookworm species: A. duodenale, A. ceylanicum, and N. americanus, nested PCR and gene sequencing were employed in fecal samples from suspected individuals residing in Thanh Binh commune, Tan Bien district, Tay Ninh province. Larvae were collected and identified by morphology before 65 DNA samples were extracted and analysed using molecular biology techniques. Results showed that 67.7% (44/65) of the samples were infected with N. americanus, while 16.9% (11/65) and 1.6% (1/65) were infected with A. ceylanicumand A. duodenale, respectively. Co-infection was present in 13.8% of cases, with A. ceylanicum and N. americanus being the most common combination. The origin of the N. americanus species identified was found to be closely related to that reported in Malaysia, while A. ceylanicum was more closely related to the species reported in Thailand. In conclusion, the study found that the new hookworm species A. ceylanicum was more prevalent than the traditional species A. duodenale in Thanh Binh commune (95.2 vs 4.8% of hookworm infections).
钩虫感染在越南很常见,由两种已知的病原体引起:Necator americanus 和 Ancylostoma duodenale。最近的研究发现了一种新的钩虫--宿根钩虫(Ancylostoma ceylanicum),这种钩虫通常寄生在狗身上。为了确定这三种钩虫的流行率,我们对这三种钩虫进行了研究:为了确定这三种钩虫(A. duodenale、A. ceylanicum 和 N. americanus)的流行情况,我们对居住在西宁省 Tan Bien 县 Thanh Binh 乡的疑似个体的粪便样本进行了巢式 PCR 和基因测序。在使用分子生物学技术提取和分析 65 份 DNA 样本之前,先收集幼虫并通过形态学进行鉴定。结果表明,67.7%(44/65)的样本感染了美洲金龟子,16.9%(11/65)和 1.6%(1/65)的样本分别感染了疟原虫和杜氏疟原虫。13.8%的病例存在合并感染,其中最常见的组合是A. ceylanicum和N. americanus。经鉴定,N. americanus 的来源与马来西亚报告的物种关系密切,而 A. ceylanicum 与泰国报告的物种关系更为密切。总之,该研究发现,在清平乡,新钩虫种类 A. ceylanicum 比传统种类 A. duodenale 更为普遍(在钩虫感染中分别占 95.2% 和 4.8%)。
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引用次数: 0
Detection of α-thalassemia mutations by Multiplex ligation-dependent probe amplification 通过多重连接依赖性探针扩增检测α地中海贫血突变
Pub Date : 2023-12-25 DOI: 10.31276/vjst.65(12).01-05
T. Le, Vu Viet Ha Vuong, Van Khanh Tran
Vietnam is a country with a high rate of people carrying α-globin gene mutations, therefore, it is highly important to screen for α-thalassemia carriers, especially in couples with one diagnosed α-carrier, in order to prevent and avoid having α-thalassemia major offspring. α-thalassemia is an inherited autosomal recessive disease, of which the phenotype depends on the degree of α-globin chain deficiency. Research samples were collected and analysed gene mutations at the Gene - Protein Research Center, Hanoi Medical University. With the objective of screening for deletional and nondeletional α-globin gene by using Multiplex ligation-dependent probe amplification (MLPA) method on eighty five people, whose spouses were α-thalassemia carriers, the results of this study have successfully identified 38/85 carriers including thirty five deletional carriers (26 --SEA, 5 -α3.7, 1 -α4.2, 1 --THAI, 1 whole α-globin deletion, and 1 POLR3K-ITFG3 gene deletion) and 3 cases of non-deletional mutation (2 -αHbCs, 1 anti3.7). Deletional mutations account for 92.1% while non-deletional mutations account for only 7.9%.
越南是一个α-球蛋白基因突变携带率很高的国家,因此,筛查α-地中海贫血症携带者非常重要,尤其是在有一名确诊α-地中海贫血症携带者的夫妇中,以预防和避免后代患上重型α-地中海贫血症。河内医科大学基因-蛋白质研究中心收集了研究样本并对基因突变进行了分析。这项研究采用多重连接依赖性探针扩增(MLPA)方法,对 85 名配偶为α-地中海贫血症携带者的人进行了α-球蛋白基因缺失和非缺失筛查,结果成功鉴定出 38/85 名携带者,其中包括 35 名缺失携带者(26 --SEA、5 -α3.7、1 个 -α4.2、1 个 -THAI、1 个全α-球蛋白缺失和 1 个 POLR3K-ITFG3 基因缺失)和 3 例非缺失突变(2 个 -αHbC、1 个 anti3.7)。缺失突变占 92.1%,而非缺失突变仅占 7.9%。
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引用次数: 0
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Ministry of Science and Technology, Vietnam
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