Pub Date : 2023-12-25DOI: 10.31276/vjst.65(12).64-69
Thi Thao Ninh, Hong Thuong Nguyen, Truong Son Dinh, Thi Dung Pham, Thi Hue Nong
This study aims to examine the in vitro propagation of Philodendron hederaceum “Lemon Lime”. The results showed that MS medium supplemented with 1.0 mg/l BA was suitable for the rapid multiplication stage, with the rate of shoot proliferation being 2.73 times, the shoots being 5.38 cm in height and the number of leaves/shoot was 4.33 after 6 weeks of culture. In the next stage, in vitro shoots were transferred to MS medium containing 0.25 mg/l IBA for rooting. Within five weeks of culture, 100% of shoots induced roots with a mean of 3.9 roots/plant and the root length was 11.04 cm. In the acclimatisation phase, plantlets achieved 100% ex vitro survival, increased by 2.19 cm in height and 1.96 leaves after four-week acclimatisation in a substrate composed of peatmoss:soil:coconut husk (1:1:1). These research results could be used to cultivate this plant with high proliferation rate and good commercial quality providing seedlings for the floriculture market in Vietnam.
本研究旨在考察 "柠檬莱姆"（Philodendron hederaceum "Lemon Lime"）的离体繁殖。结果表明，添加 1.0 毫克/升 BA 的 MS 培养基适用于快速繁殖阶段，培养 6 周后，芽的增殖率为 2.73 倍，芽的高度为 5.38 厘米，叶片数/芽为 4.33。下一阶段，将离体芽转移到含 0.25 毫克/升 IBA 的 MS 培养基中进行生根。在 5 周的培养过程中，100% 的芽都生出了根，平均每株生根 3.9 根，根长 11.04 厘米。在适应阶段，在泥炭藓：土壤：椰子壳（1:1:1）组成的基质中适应四周后，小植株的离体存活率达到 100%，高度增加了 2.19 厘米，叶片增加了 1.96 片。这些研究成果可用于培育这种具有高增殖率和良好商业品质的植物，为越南花卉市场提供种苗。
{"title":"In vitro propagation of Philodendron hederaceum “Lemon Lime”","authors":"Thi Thao Ninh, Hong Thuong Nguyen, Truong Son Dinh, Thi Dung Pham, Thi Hue Nong","doi":"10.31276/vjst.65(12).64-69","DOIUrl":"https://doi.org/10.31276/vjst.65(12).64-69","url":null,"abstract":"This study aims to examine the in vitro propagation of Philodendron hederaceum “Lemon Lime”. The results showed that MS medium supplemented with 1.0 mg/l BA was suitable for the rapid multiplication stage, with the rate of shoot proliferation being 2.73 times, the shoots being 5.38 cm in height and the number of leaves/shoot was 4.33 after 6 weeks of culture. In the next stage, in vitro shoots were transferred to MS medium containing 0.25 mg/l IBA for rooting. Within five weeks of culture, 100% of shoots induced roots with a mean of 3.9 roots/plant and the root length was 11.04 cm. In the acclimatisation phase, plantlets achieved 100% ex vitro survival, increased by 2.19 cm in height and 1.96 leaves after four-week acclimatisation in a substrate composed of peatmoss:soil:coconut husk (1:1:1). These research results could be used to cultivate this plant with high proliferation rate and good commercial quality providing seedlings for the floriculture market in Vietnam.","PeriodicalId":18650,"journal":{"name":"Ministry of Science and Technology, Vietnam","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-12-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139159987","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-12-25DOI: 10.31276/vjst.65(12).15-18
Dang Hung Nguyen, Duc Huan Pham, Hoang Nguyen
Objectives: This study was carried out to evaluate surgical outcomes of laparoscopic Heller-Toupet surgery for achalasia. Methodology: This is a retrospective case series implemented at the Gastrointestinal Department, Hanoi Medical University Hospital between January 2015 and August 2022, including 71 patients with achalasia who underwent laparoscopic Heller-Toupet. Results: 52.1% of patients in the study are male (more than female 47.9%). The average age of the cohort was 44.9±13.9 years. The incidence of dysphagia was 100%. Most patients report esophageal dilatation grade II (76.1%) and disease severity stage I (57.7%). The mean muscle opening length was 7.4±1.3 cm, the operative time was 135±25 minutes. There was no conversion, no intra-and postoperative adverse events. Long-term outcomes: good (no or very mild dysphagia) was 90.1%, moderate (moderate dysphagia) was 9.9% and clinical incidence of reflux syndrome was 17%. There was no case requiring intervention or re-operation. Conclusions: Laparoscopic Heller-Toupet is a safe, low rate of intra- and postoperative complication, and brings good long-term outcomes in the treatment of achalasia.
研究目的本研究旨在评估腹腔镜 Heller-Toupet 手术治疗贲门失弛缓症的效果。研究方法：这是一项回顾性病例系列研究，于2015年1月至2022年8月期间在河内医科大学附属医院胃肠科实施，包括71名接受腹腔镜Heller-Toupet手术的贲门失弛缓症患者。研究结果52.1%的患者为男性（47.9%多于女性）。平均年龄为（44.9±13.9）岁。吞咽困难发生率为 100%。大多数患者的食管扩张程度为 II 级（76.1%），疾病严重程度为 I 级（57.7%）。平均肌肉开口长度为（7.4±1.3）厘米，手术时间为（135±25）分钟。无转归，术中术后无不良反应。长期疗效：良好（无或极轻度吞咽困难）占90.1%，中度（中度吞咽困难）占9.9%，反流综合征的临床发生率为17%。没有需要干预或再次手术的病例。结论腹腔镜海勒-图佩特术是一种安全、术中和术后并发症发生率低、长期疗效好的贲门失弛缓症治疗方法。
{"title":"Results of laparoscopic Heller-Toupet operation for achalasia","authors":"Dang Hung Nguyen, Duc Huan Pham, Hoang Nguyen","doi":"10.31276/vjst.65(12).15-18","DOIUrl":"https://doi.org/10.31276/vjst.65(12).15-18","url":null,"abstract":"Objectives: This study was carried out to evaluate surgical outcomes of laparoscopic Heller-Toupet surgery for achalasia. Methodology: This is a retrospective case series implemented at the Gastrointestinal Department, Hanoi Medical University Hospital between January 2015 and August 2022, including 71 patients with achalasia who underwent laparoscopic Heller-Toupet. Results: 52.1% of patients in the study are male (more than female 47.9%). The average age of the cohort was 44.9±13.9 years. The incidence of dysphagia was 100%. Most patients report esophageal dilatation grade II (76.1%) and disease severity stage I (57.7%). The mean muscle opening length was 7.4±1.3 cm, the operative time was 135±25 minutes. There was no conversion, no intra-and postoperative adverse events. Long-term outcomes: good (no or very mild dysphagia) was 90.1%, moderate (moderate dysphagia) was 9.9% and clinical incidence of reflux syndrome was 17%. There was no case requiring intervention or re-operation. Conclusions: Laparoscopic Heller-Toupet is a safe, low rate of intra- and postoperative complication, and brings good long-term outcomes in the treatment of achalasia.","PeriodicalId":18650,"journal":{"name":"Ministry of Science and Technology, Vietnam","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-12-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139159529","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-12-25DOI: 10.31276/vjst.65(12).19-22
Thi Ngoan Vu, Quynh Hoa Ngo
Objectives: To describe the clinical and paraclinical features of inpatients with acute bronchitis treated at Hanoi Hospital of Traditional Medical. Subjects and methods: A cross-sectional study was conducted on 60 acute bronchitis patients from April to December 2021. Eligible patients included in the study were carried out with clinical examination, blood test, and chest X-ray. Results: The mean age in the study was 66.78±18.33 years old; the patients over 70 years old are 56.67%; the female/male ratio is 1/1.03; 21.70% of patients had smoking tobacco and pipe tobacco. Cough, thick sticky yellow sputum difficult to expectorate, and crackles were common symptoms in all patients in this study. Fever, dyspnea, and chest pain while coughing accounted for 63.30, 28.33, and 46.67%. Most of the patients had a red tongue, yellow tongue moss, and float fast pulse. Peripheral blood tests showed that increased amount of leukocytes, mainly in neutrophils. Erythrocyte sedimentation rate and C-reactive protein (CRP) increased with mean values of 36.33±15.47 mm and 25.49±16.08 mg/l.
目的描述河内传统医学医院收治的急性支气管炎住院患者的临床和辅助临床特征。研究对象和方法：2021 年 4 月至 12 月期间，对 60 名急性支气管炎患者进行了横断面研究。对符合条件的患者进行了临床检查、血液检测和胸部 X 光检查。研究结果平均年龄为（66.78±18.33）岁；70 岁以上患者占 56.67%；男女比例为 1/1.03；21.70%的患者吸烟及烟斗。咳嗽、难以排出的黄色粘稠痰液和噼啪声是所有患者的常见症状。发热、呼吸困难和咳嗽时胸痛分别占 63.30%、28.33% 和 46.67%。大多数患者舌质红，舌苔黄，脉浮快。外周血检查显示白细胞增多，以中性粒细胞为主。红细胞沉降率和 C 反应蛋白（CRP）升高，平均值分别为（36.33±15.47）毫米和（25.49±16.08）毫克/升。
{"title":"Clinical and paraclinical characteristics of acute bronchitis patients at Hanoi Hospital of Traditional Medical","authors":"Thi Ngoan Vu, Quynh Hoa Ngo","doi":"10.31276/vjst.65(12).19-22","DOIUrl":"https://doi.org/10.31276/vjst.65(12).19-22","url":null,"abstract":"Objectives: To describe the clinical and paraclinical features of inpatients with acute bronchitis treated at Hanoi Hospital of Traditional Medical. Subjects and methods: A cross-sectional study was conducted on 60 acute bronchitis patients from April to December 2021. Eligible patients included in the study were carried out with clinical examination, blood test, and chest X-ray. Results: The mean age in the study was 66.78±18.33 years old; the patients over 70 years old are 56.67%; the female/male ratio is 1/1.03; 21.70% of patients had smoking tobacco and pipe tobacco. Cough, thick sticky yellow sputum difficult to expectorate, and crackles were common symptoms in all patients in this study. Fever, dyspnea, and chest pain while coughing accounted for 63.30, 28.33, and 46.67%. Most of the patients had a red tongue, yellow tongue moss, and float fast pulse. Peripheral blood tests showed that increased amount of leukocytes, mainly in neutrophils. Erythrocyte sedimentation rate and C-reactive protein (CRP) increased with mean values of 36.33±15.47 mm and 25.49±16.08 mg/l.","PeriodicalId":18650,"journal":{"name":"Ministry of Science and Technology, Vietnam","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-12-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139158991","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-12-25DOI: 10.31276/vjst.65(12).10-14
Thi Le Nguyen, Thi Phuong Thanh Hoang, Thi Thu Hang Hoang, Tuan Hung Phan
Background: In modern life, diffuse alveolar haemorrhage (DAH) is a serious and rare condition in children. Due to the subtle clinical manifestations and diverse causes of the disease, it is easy to be missed or diagnosed late. This study describes clinical and paraclinical characteristics, with the aim to find signs suggesting early diagnosis as well as determine the cause of DAH in children. Objects and methods: Case series. Results: From January 2018 to March 2023, there were 14 children diagnosed with DAH. The age of onset is 4.08 (1.58-5.41) years old, but most patients are diagnosed late with age at diagnosis being 5.25 (1.67-6.16) years old, male/female ratio is 1:1.8. The most common symptoms reported were anaemia (100%), hemoptysis (57.1%), shortness of breath (71.4%), and respiratory failure (85.7%). The common X-ray finding was diffuse infiltrates in both lungs (92.9%). On chest computed tomography scans, 92.9% of patients exhibited images of blurred opacity spreading, 57.1% showed interlobar pleural thickening, and 28.6% presented with images of lung consolidation. 3 patients (22%) had milk allergy, 2 patients (14%) had ANCA (Anti-neutrophil cytoplasmic antibody) vasculitis, and 9 patients (64%) had idiopathic pulmonary haemosiderosis (IPH).
背景：在现代生活中，弥漫性肺泡出血（DAH）是一种严重而罕见的儿童疾病。由于该病临床表现隐匿，病因多样，很容易被漏诊或晚诊。本研究描述了该病的临床和辅助临床特征，旨在发现提示早期诊断的体征，并确定儿童 DAH 的病因。对象和方法：病例系列。结果：自2018年1月至2023年3月，共有14名儿童确诊为DAH。发病年龄为 4.08（1.58-5.41）岁，但大多数患者确诊较晚，确诊年龄为 5.25（1.67-6.16）岁，男女比例为 1:1.8。最常见的症状是贫血（100%）、咯血（57.1%）、气短（71.4%）和呼吸衰竭（85.7%）。常见的 X 光检查结果是双肺弥漫性浸润（92.9%）。在胸部计算机断层扫描中，92.9%的患者表现为模糊的翳扩散图像，57.1%的患者表现为叶间胸膜增厚，28.6%的患者表现为肺部合并症图像。3名患者（22%）对牛奶过敏，2名患者（14%）患有ANCA（抗中性粒细胞胞浆抗体）血管炎，9名患者（64%）患有特发性肺血肿（IPH）。
{"title":"Clinical features, paraclinical characteristics, and etiology of mediastinal bleeding diffusion in children at the Vietnam National Children’s Hospital from 2018 to 2023","authors":"Thi Le Nguyen, Thi Phuong Thanh Hoang, Thi Thu Hang Hoang, Tuan Hung Phan","doi":"10.31276/vjst.65(12).10-14","DOIUrl":"https://doi.org/10.31276/vjst.65(12).10-14","url":null,"abstract":"Background: In modern life, diffuse alveolar haemorrhage (DAH) is a serious and rare condition in children. Due to the subtle clinical manifestations and diverse causes of the disease, it is easy to be missed or diagnosed late. This study describes clinical and paraclinical characteristics, with the aim to find signs suggesting early diagnosis as well as determine the cause of DAH in children. Objects and methods: Case series. Results: From January 2018 to March 2023, there were 14 children diagnosed with DAH. The age of onset is 4.08 (1.58-5.41) years old, but most patients are diagnosed late with age at diagnosis being 5.25 (1.67-6.16) years old, male/female ratio is 1:1.8. The most common symptoms reported were anaemia (100%), hemoptysis (57.1%), shortness of breath (71.4%), and respiratory failure (85.7%). The common X-ray finding was diffuse infiltrates in both lungs (92.9%). On chest computed tomography scans, 92.9% of patients exhibited images of blurred opacity spreading, 57.1% showed interlobar pleural thickening, and 28.6% presented with images of lung consolidation. 3 patients (22%) had milk allergy, 2 patients (14%) had ANCA (Anti-neutrophil cytoplasmic antibody) vasculitis, and 9 patients (64%) had idiopathic pulmonary haemosiderosis (IPH).","PeriodicalId":18650,"journal":{"name":"Ministry of Science and Technology, Vietnam","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-12-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139159477","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-12-25DOI: 10.31276/vjst.65(12).34-37
Le Bao Ha Tran, Nguyen Vu Doan, Thi Ngoc My Nguyen, Minh Quan To, Thi Vy Tuyet Le, Thuan Nho Nguyen, Thanh Vy Truong, Thi Hong Tham Nguyen, Thi Thanh Tam Vu, H. Phan, Quang Tri Le
Objective:The tight connection between the cell-extracellular matrix (ECM - Extracellular matrix) is the foundation of tissue development and repair. The extracellular framework simulates the biological properties of organism-based ECM. The ECM of the skin’s dermis is a natural material, containing ingredients ideal for creating products that help tissue heal. This study focuses on the production of gel products from human skin ECM. The skin ECM is gelled by dissolving in a pepsin enzyme solution.Method: 3D gel formation process using neutral enzyme solution and incubation under physiological conditions. Gel samples were evaluated for speed and formability. Additionally, the dermal gel preparation was evaluated for its effects on the survival and proliferation of human fibroblasts in vitro by toxicity testing and proliferation testing, for 10 days. Results: Direct and extract toxicity assessments showed that the ECM gel regimen was safe for fibroblast survival. At the same time, fibroblasts had proliferation properties after 10 days in culture conditions with gel extract.Conclusion: These results showed that the ECM gel product regimen has the ability to positively impact the biological properties of cells and has the potential for application in the field of regenerative medicine.
{"title":"Fabrication and evaluation of some characteristics of the skin's dermis gel direction to application in regenerative medicine","authors":"Le Bao Ha Tran, Nguyen Vu Doan, Thi Ngoc My Nguyen, Minh Quan To, Thi Vy Tuyet Le, Thuan Nho Nguyen, Thanh Vy Truong, Thi Hong Tham Nguyen, Thi Thanh Tam Vu, H. Phan, Quang Tri Le","doi":"10.31276/vjst.65(12).34-37","DOIUrl":"https://doi.org/10.31276/vjst.65(12).34-37","url":null,"abstract":"Objective:The tight connection between the cell-extracellular matrix (ECM - Extracellular matrix) is the foundation of tissue development and repair. The extracellular framework simulates the biological properties of organism-based ECM. The ECM of the skin’s dermis is a natural material, containing ingredients ideal for creating products that help tissue heal. This study focuses on the production of gel products from human skin ECM. The skin ECM is gelled by dissolving in a pepsin enzyme solution.Method: 3D gel formation process using neutral enzyme solution and incubation under physiological conditions. Gel samples were evaluated for speed and formability. Additionally, the dermal gel preparation was evaluated for its effects on the survival and proliferation of human fibroblasts in vitro by toxicity testing and proliferation testing, for 10 days. Results: Direct and extract toxicity assessments showed that the ECM gel regimen was safe for fibroblast survival. At the same time, fibroblasts had proliferation properties after 10 days in culture conditions with gel extract.Conclusion: These results showed that the ECM gel product regimen has the ability to positively impact the biological properties of cells and has the potential for application in the field of regenerative medicine.","PeriodicalId":18650,"journal":{"name":"Ministry of Science and Technology, Vietnam","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-12-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139159484","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-12-25DOI: 10.31276/vjst.65(12).43-51
H. N. Nguyen, Trong Binh Phan, Thi Minh Nguyet Nguyen, Duc-Binh Nguyen, T. Pham, Thi Thanh Pham
This study synthesised and analysed water environmental parameters data of water supply of brackish water shrimp farming in Northern Vietnam over 5 years from 2017 to 2021 to find out the changing patterns of the parameters, thereby making appropriate recommendations for stakeholders. There were 7 analysed and evaluated water environment parameters, including water temperature, dissolved oxygen (DO), salinity, pH, alkalinity, chemical oxygen demand (COD), nitrite (N-NO2), with monitoring frequency of 2 times/month, 10 months/year at 13 monitoring locations for brackish water shrimp farming in 6 provinces: Nam Dinh, Nghe An, Ha Tinh, Quang Binh, Quang Tri, and Thua Thien Hue. Water temperature was low at the beginning of the year, gradually increased to the highest level in June, then gradually decreased to the end of the year. The temperature fluctuated strongly at the beginning and end of the year and tended to increase gradually from the North to the South. DO tended to decrease from the beginning of the year to the end of the year. Salinity was lower in the rainy season, especially in Nghe An and Quang Tri, compared to the others. The pH and alkalinity levels were suitable for brackish shrimp farming. COD in Ha Tinh and Nam Dinh, NO2 in Nam Dinh were often high, indicating that the supply water was contaminated with organic matter and needed a long-term solution.
{"title":"Changes in basic water environmental parameters of the water intake of brackish water shrimp farming in Northern Vietnam period 2017-2021","authors":"H. N. Nguyen, Trong Binh Phan, Thi Minh Nguyet Nguyen, Duc-Binh Nguyen, T. Pham, Thi Thanh Pham","doi":"10.31276/vjst.65(12).43-51","DOIUrl":"https://doi.org/10.31276/vjst.65(12).43-51","url":null,"abstract":"This study synthesised and analysed water environmental parameters data of water supply of brackish water shrimp farming in Northern Vietnam over 5 years from 2017 to 2021 to find out the changing patterns of the parameters, thereby making appropriate recommendations for stakeholders. There were 7 analysed and evaluated water environment parameters, including water temperature, dissolved oxygen (DO), salinity, pH, alkalinity, chemical oxygen demand (COD), nitrite (N-NO2), with monitoring frequency of 2 times/month, 10 months/year at 13 monitoring locations for brackish water shrimp farming in 6 provinces: Nam Dinh, Nghe An, Ha Tinh, Quang Binh, Quang Tri, and Thua Thien Hue. Water temperature was low at the beginning of the year, gradually increased to the highest level in June, then gradually decreased to the end of the year. The temperature fluctuated strongly at the beginning and end of the year and tended to increase gradually from the North to the South. DO tended to decrease from the beginning of the year to the end of the year. Salinity was lower in the rainy season, especially in Nghe An and Quang Tri, compared to the others. The pH and alkalinity levels were suitable for brackish shrimp farming. COD in Ha Tinh and Nam Dinh, NO2 in Nam Dinh were often high, indicating that the supply water was contaminated with organic matter and needed a long-term solution.","PeriodicalId":18650,"journal":{"name":"Ministry of Science and Technology, Vietnam","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-12-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139159669","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-12-25DOI: 10.31276/vjst.65(12).06-09
Thi Lan Anh Ta, Thi Que Trinh, T. Vu, Thi Thu Hien Dao, Thi Ngoc Lan Hoang, Thi Kim Phuong Doan
Objective: Survey some factors affecting Glucose-6-phosphate dehydrogenase (G6PD) enzyme activity from newborn heel blood samples and analyse screening results of some cases of G6PD enzyme deficiency. Research method: Retrospective and prospective cross-sectional description combined with case description. Results: Research on 3223 children who performed the newborn heel blood screening test from September 2022 to September 2023 showed that the high-risk rate of G6PD enzyme deficiency in boys is significantly higher than in girls. The difference is statistically significant when comparing the proportion of neonates with high-risk screening results in the Muong ethnic group and other ethnic minorities compared to the Kinh ethnic group (p<0.01). Between different sampling age groups and different weight groups, the proportion of children with enzyme activity at the high-risk threshold for G6PD enzyme deficiency did not have a statistically significant difference (p>0.05). The mutation encountered in the study was G6PD Union (c.1360C>T) (p.Arg454Cys), the enzyme activity decreased by <10% corresponding to the level of class II enzyme deficiency, showing mild hemolysis and no severe consequences in infancy. Conclusion: Children's gender and ethnicity are related to enzyme activity at risk thresholds for G6PD enzyme deficiency. Studying the correlation between genotype and phenotype is of great significance in genetic counselling, predicting prognosis and managing G6PD enzyme deficiency.
{"title":"Research of newborn screening of Glucose-6-phosphate dehydrogenase (G6PD) deficiency at Medlatec General Hospital","authors":"Thi Lan Anh Ta, Thi Que Trinh, T. Vu, Thi Thu Hien Dao, Thi Ngoc Lan Hoang, Thi Kim Phuong Doan","doi":"10.31276/vjst.65(12).06-09","DOIUrl":"https://doi.org/10.31276/vjst.65(12).06-09","url":null,"abstract":"Objective: Survey some factors affecting Glucose-6-phosphate dehydrogenase (G6PD) enzyme activity from newborn heel blood samples and analyse screening results of some cases of G6PD enzyme deficiency. Research method: Retrospective and prospective cross-sectional description combined with case description. Results: Research on 3223 children who performed the newborn heel blood screening test from September 2022 to September 2023 showed that the high-risk rate of G6PD enzyme deficiency in boys is significantly higher than in girls. The difference is statistically significant when comparing the proportion of neonates with high-risk screening results in the Muong ethnic group and other ethnic minorities compared to the Kinh ethnic group (p<0.01). Between different sampling age groups and different weight groups, the proportion of children with enzyme activity at the high-risk threshold for G6PD enzyme deficiency did not have a statistically significant difference (p>0.05). The mutation encountered in the study was G6PD Union (c.1360C>T) (p.Arg454Cys), the enzyme activity decreased by <10% corresponding to the level of class II enzyme deficiency, showing mild hemolysis and no severe consequences in infancy. Conclusion: Children's gender and ethnicity are related to enzyme activity at risk thresholds for G6PD enzyme deficiency. Studying the correlation between genotype and phenotype is of great significance in genetic counselling, predicting prognosis and managing G6PD enzyme deficiency.","PeriodicalId":18650,"journal":{"name":"Ministry of Science and Technology, Vietnam","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-12-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139158030","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-12-25DOI: 10.31276/vjst.65(12).56-63
N. G. Khong, Thi Nhu Le
In this paper, the research focuses on determining some components and culture conditions in order to optimise the 4 main steps of somatic embryogenesis and embryo regeneration by Bioreactor of two Arabica F1 coffee varieties H1 and H16, including: (1) formation of embryogenic callus, (2) multiplication of embryogenic cells, (3) embryonic differentiation, and (4) regeneration of somatic embryo using the temporary immersion Bioreactor RITA 1L. The results showed that on the medium supplemented with Kinetin 2 mg/ll and Phytagel 4 g/l, the callus formation rate reached 94.2 and 92.1% for 2 varieties H1 and H16, respectively. Besides, the addition of Glycine 20 mg/l to the culture medium increased the embryogenic callus rate by 58.1% in both varieties. Adding Trichostatin A 0.03 mg/l in the embryo differentiation medium in the first 7 days of culture showed that the number of torpedo embryos was scored from 1g of initial callus up to 1287 in H1 and 1635 in H16. Finally, using Bioreactor 1L-RITA, soaking for 1 min every 6 hours, the regeneration efficiency reached 75.02% in H1 and 82.03% in H16.
{"title":"Study on rapid multiplication of two hybrid Arabica coffee varieties F1 Centroamericano (H1) and Mundo Maya (H16) through somatic embryogenesis and Bioreactor technology","authors":"N. G. Khong, Thi Nhu Le","doi":"10.31276/vjst.65(12).56-63","DOIUrl":"https://doi.org/10.31276/vjst.65(12).56-63","url":null,"abstract":"In this paper, the research focuses on determining some components and culture conditions in order to optimise the 4 main steps of somatic embryogenesis and embryo regeneration by Bioreactor of two Arabica F1 coffee varieties H1 and H16, including: (1) formation of embryogenic callus, (2) multiplication of embryogenic cells, (3) embryonic differentiation, and (4) regeneration of somatic embryo using the temporary immersion Bioreactor RITA 1L. The results showed that on the medium supplemented with Kinetin 2 mg/ll and Phytagel 4 g/l, the callus formation rate reached 94.2 and 92.1% for 2 varieties H1 and H16, respectively. Besides, the addition of Glycine 20 mg/l to the culture medium increased the embryogenic callus rate by 58.1% in both varieties. Adding Trichostatin A 0.03 mg/l in the embryo differentiation medium in the first 7 days of culture showed that the number of torpedo embryos was scored from 1g of initial callus up to 1287 in H1 and 1635 in H16. Finally, using Bioreactor 1L-RITA, soaking for 1 min every 6 hours, the regeneration efficiency reached 75.02% in H1 and 82.03% in H16.","PeriodicalId":18650,"journal":{"name":"Ministry of Science and Technology, Vietnam","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-12-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139158004","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-12-25DOI: 10.31276/vjst.65(12).23-27
Duc Vinh Le, Trinh Vuong Tran, Kim Thach Nguyen
Hookworm infections are common in Vietnam and are caused by two known pathogens: Necator americanus and Ancylostoma duodenale. Recent studies have identified a new hookworm species - Ancylostoma ceylanicum, which typically parasitises in dogs. To determine the prevalence of these three hookworm species: A. duodenale, A. ceylanicum, and N. americanus, nested PCR and gene sequencing were employed in fecal samples from suspected individuals residing in Thanh Binh commune, Tan Bien district, Tay Ninh province. Larvae were collected and identified by morphology before 65 DNA samples were extracted and analysed using molecular biology techniques. Results showed that 67.7% (44/65) of the samples were infected with N. americanus, while 16.9% (11/65) and 1.6% (1/65) were infected with A. ceylanicumand A. duodenale, respectively. Co-infection was present in 13.8% of cases, with A. ceylanicum and N. americanus being the most common combination. The origin of the N. americanus species identified was found to be closely related to that reported in Malaysia, while A. ceylanicum was more closely related to the species reported in Thailand. In conclusion, the study found that the new hookworm species A. ceylanicum was more prevalent than the traditional species A. duodenale in Thanh Binh commune (95.2 vs 4.8% of hookworm infections).
钩虫感染在越南很常见，由两种已知的病原体引起：Necator americanus 和 Ancylostoma duodenale。最近的研究发现了一种新的钩虫--宿根钩虫（Ancylostoma ceylanicum），这种钩虫通常寄生在狗身上。为了确定这三种钩虫的流行率，我们对这三种钩虫进行了研究：为了确定这三种钩虫（A. duodenale、A. ceylanicum 和 N. americanus）的流行情况，我们对居住在西宁省 Tan Bien 县 Thanh Binh 乡的疑似个体的粪便样本进行了巢式 PCR 和基因测序。在使用分子生物学技术提取和分析 65 份 DNA 样本之前，先收集幼虫并通过形态学进行鉴定。结果表明，67.7%（44/65）的样本感染了美洲金龟子，16.9%（11/65）和 1.6%（1/65）的样本分别感染了疟原虫和杜氏疟原虫。13.8%的病例存在合并感染，其中最常见的组合是A. ceylanicum和N. americanus。经鉴定，N. americanus 的来源与马来西亚报告的物种关系密切，而 A. ceylanicum 与泰国报告的物种关系更为密切。总之，该研究发现，在清平乡，新钩虫种类 A. ceylanicum 比传统种类 A. duodenale 更为普遍（在钩虫感染中分别占 95.2% 和 4.8%）。
{"title":"Determination of the composition of hookworm species (Ancylostoma duodenale, Ancylostoma ceylanicum)and Necator americanus in humans at community in Tay Ninh province by biomolecular technique","authors":"Duc Vinh Le, Trinh Vuong Tran, Kim Thach Nguyen","doi":"10.31276/vjst.65(12).23-27","DOIUrl":"https://doi.org/10.31276/vjst.65(12).23-27","url":null,"abstract":"Hookworm infections are common in Vietnam and are caused by two known pathogens: Necator americanus and Ancylostoma duodenale. Recent studies have identified a new hookworm species - Ancylostoma ceylanicum, which typically parasitises in dogs. To determine the prevalence of these three hookworm species: A. duodenale, A. ceylanicum, and N. americanus, nested PCR and gene sequencing were employed in fecal samples from suspected individuals residing in Thanh Binh commune, Tan Bien district, Tay Ninh province. Larvae were collected and identified by morphology before 65 DNA samples were extracted and analysed using molecular biology techniques. Results showed that 67.7% (44/65) of the samples were infected with N. americanus, while 16.9% (11/65) and 1.6% (1/65) were infected with A. ceylanicumand A. duodenale, respectively. Co-infection was present in 13.8% of cases, with A. ceylanicum and N. americanus being the most common combination. The origin of the N. americanus species identified was found to be closely related to that reported in Malaysia, while A. ceylanicum was more closely related to the species reported in Thailand. In conclusion, the study found that the new hookworm species A. ceylanicum was more prevalent than the traditional species A. duodenale in Thanh Binh commune (95.2 vs 4.8% of hookworm infections).","PeriodicalId":18650,"journal":{"name":"Ministry of Science and Technology, Vietnam","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-12-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139159958","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-12-25DOI: 10.31276/vjst.65(12).01-05
T. Le, Vu Viet Ha Vuong, Van Khanh Tran
Vietnam is a country with a high rate of people carrying α-globin gene mutations, therefore, it is highly important to screen for α-thalassemia carriers, especially in couples with one diagnosed α-carrier, in order to prevent and avoid having α-thalassemia major offspring. α-thalassemia is an inherited autosomal recessive disease, of which the phenotype depends on the degree of α-globin chain deficiency. Research samples were collected and analysed gene mutations at the Gene - Protein Research Center, Hanoi Medical University. With the objective of screening for deletional and nondeletional α-globin gene by using Multiplex ligation-dependent probe amplification (MLPA) method on eighty five people, whose spouses were α-thalassemia carriers, the results of this study have successfully identified 38/85 carriers including thirty five deletional carriers (26 --SEA, 5 -α3.7, 1 -α4.2, 1 --THAI, 1 whole α-globin deletion, and 1 POLR3K-ITFG3 gene deletion) and 3 cases of non-deletional mutation (2 -αHbCs, 1 anti3.7). Deletional mutations account for 92.1% while non-deletional mutations account for only 7.9%.
{"title":"Detection of α-thalassemia mutations by Multiplex ligation-dependent probe amplification","authors":"T. Le, Vu Viet Ha Vuong, Van Khanh Tran","doi":"10.31276/vjst.65(12).01-05","DOIUrl":"https://doi.org/10.31276/vjst.65(12).01-05","url":null,"abstract":"Vietnam is a country with a high rate of people carrying α-globin gene mutations, therefore, it is highly important to screen for α-thalassemia carriers, especially in couples with one diagnosed α-carrier, in order to prevent and avoid having α-thalassemia major offspring. α-thalassemia is an inherited autosomal recessive disease, of which the phenotype depends on the degree of α-globin chain deficiency. Research samples were collected and analysed gene mutations at the Gene - Protein Research Center, Hanoi Medical University. With the objective of screening for deletional and nondeletional α-globin gene by using Multiplex ligation-dependent probe amplification (MLPA) method on eighty five people, whose spouses were α-thalassemia carriers, the results of this study have successfully identified 38/85 carriers including thirty five deletional carriers (26 --SEA, 5 -α3.7, 1 -α4.2, 1 --THAI, 1 whole α-globin deletion, and 1 POLR3K-ITFG3 gene deletion) and 3 cases of non-deletional mutation (2 -αHbCs, 1 anti3.7). Deletional mutations account for 92.1% while non-deletional mutations account for only 7.9%.","PeriodicalId":18650,"journal":{"name":"Ministry of Science and Technology, Vietnam","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-12-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139159510","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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