Pub Date : 2024-03-15DOI: 10.31276/vjste.66(1).16-23
Ngoc Danh Dang, Xuan Thiet Nguyen
Compared to conventional mechanical transmissions, hydraulic transmission has the disadvantage of lower energy efficiency. However, it is widely employed in various working machines such as construction machines, off-road vehicles, forest logging machines, and especially agricultural machinery, owing to its flexibility in design, geometric arrangement, and simplicity in operation. In the development project for carrot harvesting machinery conjugate, hydraulic transmission is the optimal solution for the design and implementation of the carrot collecting roller system. This project is oriented towards achieving a practical result where the system structure is simple and effective, utilising hydraulic components available on the market in Vietnam. Given the myriad of hydraulic transmission circuits available, this paper undertakes an analysis based on numerical simulations of the functionality and energy efficiency of potential system design options and proposes a new practical speed control circuit. This suggested circuit uses a pressure-compensated flow control valve arranged parallel to the hydraulic motor. The study is carried out in the Matlab/Simulink simulation environment, where the circuit models are constructed using hydraulic components with practical parameter settings sourced from component suppliers. The analysis results indicate that the proposed circuit strikes a balance between functionality and economic efficiency.
{"title":"Analysis of hydraulic drive circuit selection for a carrot collecting system","authors":"Ngoc Danh Dang, Xuan Thiet Nguyen","doi":"10.31276/vjste.66(1).16-23","DOIUrl":"https://doi.org/10.31276/vjste.66(1).16-23","url":null,"abstract":"Compared to conventional mechanical transmissions, hydraulic transmission has the disadvantage of lower energy efficiency. However, it is widely employed in various working machines such as construction machines, off-road vehicles, forest logging machines, and especially agricultural machinery, owing to its flexibility in design, geometric arrangement, and simplicity in operation. In the development project for carrot harvesting machinery conjugate, hydraulic transmission is the optimal solution for the design and implementation of the carrot collecting roller system. This project is oriented towards achieving a practical result where the system structure is simple and effective, utilising hydraulic components available on the market in Vietnam. Given the myriad of hydraulic transmission circuits available, this paper undertakes an analysis based on numerical simulations of the functionality and energy efficiency of potential system design options and proposes a new practical speed control circuit. This suggested circuit uses a pressure-compensated flow control valve arranged parallel to the hydraulic motor. The study is carried out in the Matlab/Simulink simulation environment, where the circuit models are constructed using hydraulic components with practical parameter settings sourced from component suppliers. The analysis results indicate that the proposed circuit strikes a balance between functionality and economic efficiency.","PeriodicalId":18650,"journal":{"name":"Ministry of Science and Technology, Vietnam","volume":"37 6","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-03-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140237418","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-03-15DOI: 10.31276/vjste.66(1).84-89
D. Chu, Hue Vu Thi, Ngoc Hoan Le
The RNeasy Mini Kit and the TRI Reagent protocol are commonly employed techniques for extracting total RNA and are widely utilised in research pertaining to the expression of adipose biomarkers. This study was undertaken to establish and determine an appropriate method for isolating RNA from frozen adipose tissue, with the objective of analysing adipose biomarkers. Total RNA was extracted from frozen white adipose tissues of mice (-80oC) using both the RNeasy Mini Kit and the TRI Reagent protocol. The concentration and purity of the RNA were assessed using NanoDrop. One-step RT-qPCR was employed to quantify the mRNA expression levels of three adipose biomarkers: MEST, SFRP5, and SCD1. The results of this investigation indicated that the RNeasy Mini Kit yielded a higher RNA concentration when compared to the TRI Reagent method. Furthermore, the RNeasy Mini Kit provided RNA samples with superior purity, as evidenced by a higher 260/230 ratio. Although RNA extracted using the TRI Reagent exhibited slightly elevated mRNA expression levels for the three biomarkers, these differences did not reach statistical significance. In summary, both methods are suitable for total RNA extraction, but the RNeasy Mini Kit is recommended for obtaining higher RNA concentration and purity. The choice of method should be contingent upon the intended downstream application of the RNA.
RNeasy Mini Kit 和 TRI Reagent 协议是提取总 RNA 的常用技术，在有关脂肪生物标志物表达的研究中被广泛使用。本研究旨在建立和确定从冷冻脂肪组织中分离 RNA 的适当方法，目的是分析脂肪生物标志物。研究人员使用 RNeasy Mini Kit 和 TRI Reagent 方法从冷冻的小鼠白色脂肪组织（-80oC）中提取总 RNA。使用 NanoDrop 对 RNA 的浓度和纯度进行了评估。采用一步式 RT-qPCR 对三种脂肪生物标志物的 mRNA 表达水平进行量化：MEST、SFRP5 和 SCD1。研究结果表明，与 TRI 试剂法相比，RNeasy Mini 试剂盒产生的 RNA 浓度更高。此外，RNeasy Mini 试剂盒提供的 RNA 样品纯度更高，260/230 比率也更高。虽然使用 TRI 试剂提取的 RNA 在三种生物标志物的 mRNA 表达水平上略有升高，但这些差异未达到统计学意义。总之，两种方法都适用于总 RNA 提取，但建议使用 RNeasy Mini 试剂盒来获得更高的 RNA 浓度和纯度。选择哪种方法应根据 RNA 的下游应用而定。
{"title":"RNA isolation by two different methods in analysing adipose-biomarkers of mouse adipose tissues","authors":"D. Chu, Hue Vu Thi, Ngoc Hoan Le","doi":"10.31276/vjste.66(1).84-89","DOIUrl":"https://doi.org/10.31276/vjste.66(1).84-89","url":null,"abstract":"The RNeasy Mini Kit and the TRI Reagent protocol are commonly employed techniques for extracting total RNA and are widely utilised in research pertaining to the expression of adipose biomarkers. This study was undertaken to establish and determine an appropriate method for isolating RNA from frozen adipose tissue, with the objective of analysing adipose biomarkers. Total RNA was extracted from frozen white adipose tissues of mice (-80oC) using both the RNeasy Mini Kit and the TRI Reagent protocol. The concentration and purity of the RNA were assessed using NanoDrop. One-step RT-qPCR was employed to quantify the mRNA expression levels of three adipose biomarkers: MEST, SFRP5, and SCD1. The results of this investigation indicated that the RNeasy Mini Kit yielded a higher RNA concentration when compared to the TRI Reagent method. Furthermore, the RNeasy Mini Kit provided RNA samples with superior purity, as evidenced by a higher 260/230 ratio. Although RNA extracted using the TRI Reagent exhibited slightly elevated mRNA expression levels for the three biomarkers, these differences did not reach statistical significance. In summary, both methods are suitable for total RNA extraction, but the RNeasy Mini Kit is recommended for obtaining higher RNA concentration and purity. The choice of method should be contingent upon the intended downstream application of the RNA.","PeriodicalId":18650,"journal":{"name":"Ministry of Science and Technology, Vietnam","volume":"116 8","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-03-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140237964","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-03-15DOI: 10.31276/vjste.66(1).59-76
Long Hoang, Mai T. T. Nguyen, D. N. Nguyen, Kim Hoang, Clair Hershey, R. Howeler
Vietnamese cassava varieties constitute the fundamental and pivotal element in the sustainable development programme for cassava. This article aims to encapsulate the advancements made over nearly five decades in breeding and enhancing Vietnamese cassava varieties. It delineates the suitable cassava variety structures for each period and ecological region. The selection of cassava varieties exhibiting high starch yield and disease resistance, coupled with the establishment of a suitable and efficient cassava cultivation model, exemplified by 10T for Vietnamese cassava varieties KM568, KM539, KM537, KM569, and KM94, stands as a cornerstone for sustaining cassava development over the years. Presently, we advocate for farmers to cultivate promising cassava varieties such as KM568 or KM539 (an enhanced version of the International Center for Tropical Agriculture (CIAT) cassava variety C39, refined through multiple breeding cycles from 2004 onwards), KM537, KM569, or HN1 (originally known as TMEB419), alongside popular cassava varieties: KM440, KM419, KM94, KM7, STB1, KM414, KM98-7, KM140, KM98-5, KM98-1. We have conducted Distinctness, Uniformity, and Stability (DUS) and Value for Cultivation and Use (VCU) tests, showcasing outstanding cassava varieties in large-scale farming, thereby providing compelling evidence for the prudent conservation and sustainable development of cassava. Vietnamese cassava progression (1975 to date) has traversed six stages, with five waves of restructuring cassava varieties, aligning with target orientations, farming conditions, and market demands, culminating in 16 popular cassava varieties and four promising cassava varieties KM568, KM539, KM537, and KM569.
{"title":"Vietnamese cassava varieties progression across 50 years","authors":"Long Hoang, Mai T. T. Nguyen, D. N. Nguyen, Kim Hoang, Clair Hershey, R. Howeler","doi":"10.31276/vjste.66(1).59-76","DOIUrl":"https://doi.org/10.31276/vjste.66(1).59-76","url":null,"abstract":"Vietnamese cassava varieties constitute the fundamental and pivotal element in the sustainable development programme for cassava. This article aims to encapsulate the advancements made over nearly five decades in breeding and enhancing Vietnamese cassava varieties. It delineates the suitable cassava variety structures for each period and ecological region. The selection of cassava varieties exhibiting high starch yield and disease resistance, coupled with the establishment of a suitable and efficient cassava cultivation model, exemplified by 10T for Vietnamese cassava varieties KM568, KM539, KM537, KM569, and KM94, stands as a cornerstone for sustaining cassava development over the years. Presently, we advocate for farmers to cultivate promising cassava varieties such as KM568 or KM539 (an enhanced version of the International Center for Tropical Agriculture (CIAT) cassava variety C39, refined through multiple breeding cycles from 2004 onwards), KM537, KM569, or HN1 (originally known as TMEB419), alongside popular cassava varieties: KM440, KM419, KM94, KM7, STB1, KM414, KM98-7, KM140, KM98-5, KM98-1. We have conducted Distinctness, Uniformity, and Stability (DUS) and Value for Cultivation and Use (VCU) tests, showcasing outstanding cassava varieties in large-scale farming, thereby providing compelling evidence for the prudent conservation and sustainable development of cassava. Vietnamese cassava progression (1975 to date) has traversed six stages, with five waves of restructuring cassava varieties, aligning with target orientations, farming conditions, and market demands, culminating in 16 popular cassava varieties and four promising cassava varieties KM568, KM539, KM537, and KM569.","PeriodicalId":18650,"journal":{"name":"Ministry of Science and Technology, Vietnam","volume":"15 3","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-03-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140237473","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-02-25DOI: 10.31276/vjst.66(2).44-48
Thi Thanh Nhan Giang, Thi Phuong Mai Pham, Van Ba Nguyen, Thi Thu Thuy Tran, Thi Quynh Chau Nguyen, Thi Hau Tran, T. A. Hoang, Khanh Van Nguyen, Q. M. Luu, Doan Lan Pham
In this study, the complete nucleotide sequence of the mitochondrial genome of the Mong chicken and an indigenous chicken breed of Vietnam, was determined by the Sanger sequencing method, structurally analysed and used to evaluate the phylogenetic relationship with different chicken breeds. The complete nucleotide sequence data of the Mong chicken’ mitochondrial genome was registered on GenBank with the accession number OR643716. The Mong chicken’ mitochondrial genome has a size of 16785 base pair (bp) and includes 22 transfer RNA genes, two ribosomal RNA genes, 13 protein-coding genes, and one non-coding control region (D-loop). A, T, G, and C nucleotide proportions were 30.28, 23.76, 13.48, and 32.48%, respectively. Results of the analysis of phylogenetic relationships based on 33 complete mitochondrial genome sequences showed that the Mong chicken has evolutional origin from the red junglefowl subspecies Gallus gallus spadiceus. The Mong chicken is in close relationship with the Dong Tao chicken and Cao Son dwarf chicken in Vietnam, Rugao yellow chicken and Xiaoxiang chicken in China.
{"title":"Analysis of the mitochondrial genome and phylogenetic relationships of Mong chicken","authors":"Thi Thanh Nhan Giang, Thi Phuong Mai Pham, Van Ba Nguyen, Thi Thu Thuy Tran, Thi Quynh Chau Nguyen, Thi Hau Tran, T. A. Hoang, Khanh Van Nguyen, Q. M. Luu, Doan Lan Pham","doi":"10.31276/vjst.66(2).44-48","DOIUrl":"https://doi.org/10.31276/vjst.66(2).44-48","url":null,"abstract":"In this study, the complete nucleotide sequence of the mitochondrial genome of the Mong chicken and an indigenous chicken breed of Vietnam, was determined by the Sanger sequencing method, structurally analysed and used to evaluate the phylogenetic relationship with different chicken breeds. The complete nucleotide sequence data of the Mong chicken’ mitochondrial genome was registered on GenBank with the accession number OR643716. The Mong chicken’ mitochondrial genome has a size of 16785 base pair (bp) and includes 22 transfer RNA genes, two ribosomal RNA genes, 13 protein-coding genes, and one non-coding control region (D-loop). A, T, G, and C nucleotide proportions were 30.28, 23.76, 13.48, and 32.48%, respectively. Results of the analysis of phylogenetic relationships based on 33 complete mitochondrial genome sequences showed that the Mong chicken has evolutional origin from the red junglefowl subspecies Gallus gallus spadiceus. The Mong chicken is in close relationship with the Dong Tao chicken and Cao Son dwarf chicken in Vietnam, Rugao yellow chicken and Xiaoxiang chicken in China.","PeriodicalId":18650,"journal":{"name":"Ministry of Science and Technology, Vietnam","volume":"24 8","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-02-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140432260","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-02-25DOI: 10.31276/vjst.66(2).60-65
Phuoc Thien Hoang Truong, Thi Thao Nhu Le, Hoang Phuc Le, Trong Nghia Tran, Uyen Tran Da Dao
The study aimed to determine the presence and composition of arbuscular mycorrhizal fungi (AMF) in the rhizospheric soils and roots of vegetable plants (14 species of leafy vegetables) grown in Hoc Mon, Cu Chi, and Binh Chanh districts, Ho Chi Minh city. The results of the isolation of AMF spores based on the wet sieving method showed the presence of AMF in the rhizospheric soils of vegetables with an average spore density of 87.6 spores/50 g of soil. For the symbiotic ratio of AMF to the root tissues, there was no symbiosis in 4 vegetable crops, including amaranth, mustard green, leaf mustard, and bok choy. Still, there was symbiosis in the remaining 10 plants, with an average symbiotic rate of 6.6%. Identification of AMF spores based on the morphological characteristics recorded in all the soil samples appeared spore phenotypes of 5 fungal genera including Glomus, Acaulospora, Gigaspora, Scutellospora, and Sclerocystis. However, 3 morphological types were not yet identified. From the obtained results, two genera, Glomus and Acaulospora, have the highest frequency at 43.9 and 39.6%, respectively.
{"title":"A survey on the composition and occurrence frequency of arbuscular mycorrhizal fungi in rhizospheric soils and roots of vegetables grown in Ho Chi Minh city","authors":"Phuoc Thien Hoang Truong, Thi Thao Nhu Le, Hoang Phuc Le, Trong Nghia Tran, Uyen Tran Da Dao","doi":"10.31276/vjst.66(2).60-65","DOIUrl":"https://doi.org/10.31276/vjst.66(2).60-65","url":null,"abstract":"The study aimed to determine the presence and composition of arbuscular mycorrhizal fungi (AMF) in the rhizospheric soils and roots of vegetable plants (14 species of leafy vegetables) grown in Hoc Mon, Cu Chi, and Binh Chanh districts, Ho Chi Minh city. The results of the isolation of AMF spores based on the wet sieving method showed the presence of AMF in the rhizospheric soils of vegetables with an average spore density of 87.6 spores/50 g of soil. For the symbiotic ratio of AMF to the root tissues, there was no symbiosis in 4 vegetable crops, including amaranth, mustard green, leaf mustard, and bok choy. Still, there was symbiosis in the remaining 10 plants, with an average symbiotic rate of 6.6%. Identification of AMF spores based on the morphological characteristics recorded in all the soil samples appeared spore phenotypes of 5 fungal genera including Glomus, Acaulospora, Gigaspora, Scutellospora, and Sclerocystis. However, 3 morphological types were not yet identified. From the obtained results, two genera, Glomus and Acaulospora, have the highest frequency at 43.9 and 39.6%, respectively.","PeriodicalId":18650,"journal":{"name":"Ministry of Science and Technology, Vietnam","volume":"30 11","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-02-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140433119","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-02-25DOI: 10.31276/vjst.66(2).75-80
Minh Tuong Le, Hong Nhieu Le, Quoc Viet Le, Quang Dung Nguyen, Van Tap Nguyen
The research was carried out at Can Tho University with the aim of investigating the ability of actinomycetes isolates to antagonise Colletotrichum sp. fungus causing anthracnose disease in durian. The results showed that among the 28 actinomycete strains evaluated, 6 actinomycetes including BT19, BL10, TG19, BT16, ĐT15, and VL9 had stronger antagonism with a radius of inhibition zone reaches 12.4, 9.4, 11.1, 9.8, 9.7, and 9.2 mm, respectively and antagonistic efficacy of 63.22, 58.62, 55.86, 53.56, 52.64, and 53.79%, respectively at 7 days after co-inoculation. The ability to degrade β-glucan of 6 strains BT19, BL10, TG19, BT16, DT15, and VL9 performed under laboratory conditions showed that the BT19 isolate had the highest β-glucanolytic activity with the β-glucan lyse halo radius of 8.53 mm and secreted β-glucanase content reached 0.678 IU/ml at 14 days after testing. The results of this study will be the basis for further research to contribute to finding effective methods to control anthracnose disease in durian.
{"title":"Antagonistic potential of actinomycete strains against Colletotrichum sp. causes anthracnose disease in durian","authors":"Minh Tuong Le, Hong Nhieu Le, Quoc Viet Le, Quang Dung Nguyen, Van Tap Nguyen","doi":"10.31276/vjst.66(2).75-80","DOIUrl":"https://doi.org/10.31276/vjst.66(2).75-80","url":null,"abstract":"The research was carried out at Can Tho University with the aim of investigating the ability of actinomycetes isolates to antagonise Colletotrichum sp. fungus causing anthracnose disease in durian. The results showed that among the 28 actinomycete strains evaluated, 6 actinomycetes including BT19, BL10, TG19, BT16, ĐT15, and VL9 had stronger antagonism with a radius of inhibition zone reaches 12.4, 9.4, 11.1, 9.8, 9.7, and 9.2 mm, respectively and antagonistic efficacy of 63.22, 58.62, 55.86, 53.56, 52.64, and 53.79%, respectively at 7 days after co-inoculation. The ability to degrade β-glucan of 6 strains BT19, BL10, TG19, BT16, DT15, and VL9 performed under laboratory conditions showed that the BT19 isolate had the highest β-glucanolytic activity with the β-glucan lyse halo radius of 8.53 mm and secreted β-glucanase content reached 0.678 IU/ml at 14 days after testing. The results of this study will be the basis for further research to contribute to finding effective methods to control anthracnose disease in durian.","PeriodicalId":18650,"journal":{"name":"Ministry of Science and Technology, Vietnam","volume":"23 12","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-02-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140432104","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-02-25DOI: 10.31276/vjst.66(2).20-25
Xuan Phuc Nguyen, Van Vien Doan, Manh Hung Tran
High blood pressure is a major cause of stroke and cardiovascular complications. The development of a hypertensive crisis model allows rapid testing of the effects of antihypertensive drugs, meeting the need for research and development of chronic pharmacological models. A model of acute hypertension was induced by intraperitoneal injections of xylometazoline at different doses and subcutaneous injections of atropine at a dose of 0.4 mg/kg. This study applied the CODA high-throughput hypertensive machine to determine a suitable model. The results of injecting xylometazoline at a dose of 1 mg/kg ( IP) and atropine (0.4 mg/kg) (SC) showed induced hypertension with systolic >130 mmHg constantly with 15 circles in 20 minutes. The hypotensive effect was found when oral administration of nifedipine (6 mg/kg), losartan (100 mg/kg), and captopril (100 mg/kg). However, the hypotensive effect of bisoprolol was not found at a dose of 50 mg/kg. This mouse model can be applied for further screening and evaluating the therapeutic effect of hypotensive drugs.
{"title":"Building a model to evaluate hypertensive crisis in mice","authors":"Xuan Phuc Nguyen, Van Vien Doan, Manh Hung Tran","doi":"10.31276/vjst.66(2).20-25","DOIUrl":"https://doi.org/10.31276/vjst.66(2).20-25","url":null,"abstract":"High blood pressure is a major cause of stroke and cardiovascular complications. The development of a hypertensive crisis model allows rapid testing of the effects of antihypertensive drugs, meeting the need for research and development of chronic pharmacological models. A model of acute hypertension was induced by intraperitoneal injections of xylometazoline at different doses and subcutaneous injections of atropine at a dose of 0.4 mg/kg. This study applied the CODA high-throughput hypertensive machine to determine a suitable model. The results of injecting xylometazoline at a dose of 1 mg/kg ( IP) and atropine (0.4 mg/kg) (SC) showed induced hypertension with systolic >130 mmHg constantly with 15 circles in 20 minutes. The hypotensive effect was found when oral administration of nifedipine (6 mg/kg), losartan (100 mg/kg), and captopril (100 mg/kg). However, the hypotensive effect of bisoprolol was not found at a dose of 50 mg/kg. This mouse model can be applied for further screening and evaluating the therapeutic effect of hypotensive drugs.","PeriodicalId":18650,"journal":{"name":"Ministry of Science and Technology, Vietnam","volume":"32 124","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-02-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140432844","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-02-25DOI: 10.31276/vjst.66(2).55-59
Van Doan Duong, Thi Thu Ha Tran, Tri-Thuc Bui, Manh Tuan Nguyen, Tien Dung Nguyen
In this study, seven clones of Acacia auriculiformis,collected in Cam Lo district, Quang Binh province were assessed for genetic diversity using 15 ISSR markers. The results showed that seven Acacia clones had an average level of genetic variation. In which, four ISSR markers including ISSR1, ISSR3, ISSR8, and ISSR15 showed higher polymorphism information content (PIC) value than the others. The results analysis indicated that 30 out of 40 DNA fragments were polymorphic (75%, PIC=0.28-0.33). The ISSR8 exhibited the highest genetic diversity index (0.33), while ISSR15 showed the lowest diversity (0.28). Seven clones of A. auriculiformis were divided into two main groups: group I comprised four clones: Clt7, Clt18, Clt19, and Clt26; group II included the remaining three clones: Clt25, Clt43, and Clt57. Genetic variation was correlated with plant growth ability. This result exhibited that the ISSR marker was an efficient tool in assessing and selecting the good genetic resource of A. auriculiformis for the forestry tree breeding program in Vietnam.
本研究利用 15 个 ISSR 标记评估了在广平省 Cam Lo 县采集的 7 个金合欢克隆的遗传多样性。结果表明，7 个金合欢克隆的遗传变异水平一般。其中，ISSR1、ISSR3、ISSR8 和 ISSR15 等四个 ISSR 标记的多态性信息含量（PIC）值高于其他标记。结果分析表明，40 个 DNA 片段中有 30 个具有多态性（75%，PIC=0.28-0.33）。其中 ISSR8 的遗传多样性指数最高（0.33），而 ISSR15 的多样性指数最低（0.28）。A. auriculiformis 的 7 个克隆被分为两大组：第一组由 4 个克隆组成：第 I 组包括四个克隆：Clt7、Clt18、Clt19 和 Clt26；第 II 组包括其余三个克隆：第二组包括其余三个克隆：Clt25、Clt43 和 Clt57。遗传变异与植物生长能力相关。这一结果表明，ISSR 标记是评估和筛选 A. auriculiformis 优良遗传资源的有效工具，可用于越南的林木育种计划。
{"title":"Identification of genetic relationships of Acacia auriculiformis clones using ISSR markers","authors":"Van Doan Duong, Thi Thu Ha Tran, Tri-Thuc Bui, Manh Tuan Nguyen, Tien Dung Nguyen","doi":"10.31276/vjst.66(2).55-59","DOIUrl":"https://doi.org/10.31276/vjst.66(2).55-59","url":null,"abstract":"In this study, seven clones of Acacia auriculiformis,collected in Cam Lo district, Quang Binh province were assessed for genetic diversity using 15 ISSR markers. The results showed that seven Acacia clones had an average level of genetic variation. In which, four ISSR markers including ISSR1, ISSR3, ISSR8, and ISSR15 showed higher polymorphism information content (PIC) value than the others. The results analysis indicated that 30 out of 40 DNA fragments were polymorphic (75%, PIC=0.28-0.33). The ISSR8 exhibited the highest genetic diversity index (0.33), while ISSR15 showed the lowest diversity (0.28). Seven clones of A. auriculiformis were divided into two main groups: group I comprised four clones: Clt7, Clt18, Clt19, and Clt26; group II included the remaining three clones: Clt25, Clt43, and Clt57. Genetic variation was correlated with plant growth ability. This result exhibited that the ISSR marker was an efficient tool in assessing and selecting the good genetic resource of A. auriculiformis for the forestry tree breeding program in Vietnam.","PeriodicalId":18650,"journal":{"name":"Ministry of Science and Technology, Vietnam","volume":"30 8","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-02-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140433122","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-02-25DOI: 10.31276/vjst.66(2).08-13
Thi Le Thuy Nguyen, Minh Tuan Nguyen, Thi Kim Tram Pham, Dang Quan Nguyen
SARS-CoV-2 infects the host through the interaction be-tween spike protein and the ACE2 (Angiotensin convert-ing enzyme 2) receptor on the host cell surface. There-fore, ACE2 is considered as a key target in drug devel-opment against COVID-19. In this study, we generated the truncated recombinant human ACE2 (trhACE2) expressed on Escherichia coli and evaluated its binding activity to spike protein of SARS-CoV-2. The sequence of ACE2 (18-119 aa) was cloned into pET28a(+) plasmid and transformed into E. coli DH5α strain and finally was transferred to E. coli BL21 (DE3) cells for protein expression. Protein trhACE2 was purified by affinity chromatography using a HisTrap HP column. Protein purity was above 95% and production yield was 75 mg/l. The purified protein was refolded by dialysis method. trhACE2 was evaluated for its ability to bind the spike protein by sandwich ELISA assay and surface plasmon resonance (SPR). The results showed that trhACE2 has the ability to bind spike protein of SARS-CoV-2 with the equilibrium dissociation constant Kd=15.7 nM. With a relatively simple and inexpensive expression and purifi-cation process on E. coli system, there is the potential to develop trhACE2 for preventive and supportive thera-pies against COVID-19.
{"title":"Expression and purification of truncated recombinant human angiotensin converting enzyme 2 (trhACE2) capable of binding spike protein of SARS-CoV-2","authors":"Thi Le Thuy Nguyen, Minh Tuan Nguyen, Thi Kim Tram Pham, Dang Quan Nguyen","doi":"10.31276/vjst.66(2).08-13","DOIUrl":"https://doi.org/10.31276/vjst.66(2).08-13","url":null,"abstract":"SARS-CoV-2 infects the host through the interaction be-tween spike protein and the ACE2 (Angiotensin convert-ing enzyme 2) receptor on the host cell surface. There-fore, ACE2 is considered as a key target in drug devel-opment against COVID-19. In this study, we generated the truncated recombinant human ACE2 (trhACE2) expressed on Escherichia coli and evaluated its binding activity to spike protein of SARS-CoV-2. The sequence of ACE2 (18-119 aa) was cloned into pET28a(+) plasmid and transformed into E. coli DH5α strain and finally was transferred to E. coli BL21 (DE3) cells for protein expression. Protein trhACE2 was purified by affinity chromatography using a HisTrap HP column. Protein purity was above 95% and production yield was 75 mg/l. The purified protein was refolded by dialysis method. trhACE2 was evaluated for its ability to bind the spike protein by sandwich ELISA assay and surface plasmon resonance (SPR). The results showed that trhACE2 has the ability to bind spike protein of SARS-CoV-2 with the equilibrium dissociation constant Kd=15.7 nM. With a relatively simple and inexpensive expression and purifi-cation process on E. coli system, there is the potential to develop trhACE2 for preventive and supportive thera-pies against COVID-19.","PeriodicalId":18650,"journal":{"name":"Ministry of Science and Technology, Vietnam","volume":"14 75","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-02-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140432683","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-02-25DOI: 10.31276/vjst.66(2).26-31
Thanh Binh Nguyen, Thi Thu Hien Nguyen, Phuong Thanh Duong, Thi Thanh Thuy Tran, Ngoc Hien Nhan, Khanh Hoa Nguyen, KC Huong Hoang, Tuan Kiet Nguyen, Minh Can Nguyen, T. Q. Ngo, Duy Thao Huynh
The human amniotic membrane has been widely used in clinical practice to treat all kinds of lesions related to the epithelial surface, corneal epithelial reconstruction, and orthopaedic trauma. Currently, another application aspect of the amniotic membrane of great interest is its use as an in vitro adherent cell culture medium. In this study, the human amniotic membranes were collected under sterile conditions in the operating room and tested negative for HIV, HBV, HCV, and VDRL. The amniotic membranes were then processed to remove the epithelial layer, to obtain a collagen membrane that can be used as a substrate for cell culture adhesion. Following that, the collagen membrane was structurally evaluated by Hematoxylin and eosin (H&E) staining, SEM. Next, we tested the use of collagen membranes as a culture medium for human fibroblasts and evaluated cell adhesion, growth, and development by histological images over time of cell culture. The image results obtained before and after processing showed that the collagen membrane has an acellular structure, maintaining the basement membrane’s structure. Cell culture results exhibited that fibroblasts adhered and developed well on this collagen membrane.
{"title":"Application of collagen membrane from amniotic membrane as a substrate for adherent cell culturing in tissue engineering","authors":"Thanh Binh Nguyen, Thi Thu Hien Nguyen, Phuong Thanh Duong, Thi Thanh Thuy Tran, Ngoc Hien Nhan, Khanh Hoa Nguyen, KC Huong Hoang, Tuan Kiet Nguyen, Minh Can Nguyen, T. Q. Ngo, Duy Thao Huynh","doi":"10.31276/vjst.66(2).26-31","DOIUrl":"https://doi.org/10.31276/vjst.66(2).26-31","url":null,"abstract":"The human amniotic membrane has been widely used in clinical practice to treat all kinds of lesions related to the epithelial surface, corneal epithelial reconstruction, and orthopaedic trauma. Currently, another application aspect of the amniotic membrane of great interest is its use as an in vitro adherent cell culture medium. In this study, the human amniotic membranes were collected under sterile conditions in the operating room and tested negative for HIV, HBV, HCV, and VDRL. The amniotic membranes were then processed to remove the epithelial layer, to obtain a collagen membrane that can be used as a substrate for cell culture adhesion. Following that, the collagen membrane was structurally evaluated by Hematoxylin and eosin (H&E) staining, SEM. Next, we tested the use of collagen membranes as a culture medium for human fibroblasts and evaluated cell adhesion, growth, and development by histological images over time of cell culture. The image results obtained before and after processing showed that the collagen membrane has an acellular structure, maintaining the basement membrane’s structure. Cell culture results exhibited that fibroblasts adhered and developed well on this collagen membrane.","PeriodicalId":18650,"journal":{"name":"Ministry of Science and Technology, Vietnam","volume":"9 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-02-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140433565","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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