Pub Date : 2025-02-11Epub Date: 2024-12-31DOI: 10.1128/mra.01054-24
Farwa Hassan, Hazrat Ali, Noor Hassan, Anam Saqib, Muhammad Hamid Rashid, Aniqa Nawaz, Rubab Zahra Naqvi
Phytopathogens with multi-drug resistance are emerging frequently, resulting in various disease outbreaks. Hence, exploring new antimicrobials is urgent. Here, we present the draft genome sequence of Streptomyces carpaticus FH1 strain, with the potential to produce various antimicrobial compounds. Genomic annotation revealed various genes encoding kanamycin, ectoine, and naringerin.
{"title":"Draft genome sequence of <i>Streptomyces carpaticus</i> FH1.","authors":"Farwa Hassan, Hazrat Ali, Noor Hassan, Anam Saqib, Muhammad Hamid Rashid, Aniqa Nawaz, Rubab Zahra Naqvi","doi":"10.1128/mra.01054-24","DOIUrl":"10.1128/mra.01054-24","url":null,"abstract":"<p><p>Phytopathogens with multi-drug resistance are emerging frequently, resulting in various disease outbreaks. Hence, exploring new antimicrobials is urgent. Here, we present the draft genome sequence of <i>Streptomyces carpaticus</i> FH1 strain, with the potential to produce various antimicrobial compounds. Genomic annotation revealed various genes encoding kanamycin, ectoine, and naringerin.</p>","PeriodicalId":18654,"journal":{"name":"Microbiology Resource Announcements","volume":" ","pages":"e0105424"},"PeriodicalIF":0.7,"publicationDate":"2025-02-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11812428/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142915431","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2025-02-11Epub Date: 2025-01-10DOI: 10.1128/mra.01025-24
Elena L Peredo, Suzanne Thomas, Zoe G Cardon
A draft genome was generated for a strain of Mycobacterium closely related to Mycobacterium sp. ENV421 isolated in vitro from plants of smooth cordgrass germinated in vitro from seeds collected in a salt marsh in Cape Cod (USA). Genomic DNA was sequenced using paired-end Illumina technologies. Here, we report the classification and genome statistics of Mycobacterium sp. SA01. A strain of Mycobacterium was isolated from seedlings of Sporobolus alterniflorus germinated under in vitro conditions after surface sterilization. The seeds were collected in Little Swippewisett Marsh, Barnstable, MA, USA.
{"title":"Draft genome sequence of <i>Mycobacterium</i> sp. SA01 isolated from <i>Sporobolus alterniflorus</i> seedlings collected in Cape Cod (USA).","authors":"Elena L Peredo, Suzanne Thomas, Zoe G Cardon","doi":"10.1128/mra.01025-24","DOIUrl":"10.1128/mra.01025-24","url":null,"abstract":"<p><p>A draft genome was generated for a strain of <i>Mycobacterium</i> closely related to <i>Mycobacterium</i> sp. ENV421 isolated <i>in vitro</i> from plants of smooth cordgrass germinated <i>in vitro</i> from seeds collected in a salt marsh in Cape Cod (USA). Genomic DNA was sequenced using paired-end Illumina technologies. Here, we report the classification and genome statistics of <i>Mycobacterium</i> sp. SA01. A strain of <i>Mycobacterium</i> was isolated from seedlings of <i>Sporobolus alterniflorus</i> germinated under in vitro conditions after surface sterilization. The seeds were collected in Little Swippewisett Marsh, Barnstable, MA, USA.</p>","PeriodicalId":18654,"journal":{"name":"Microbiology Resource Announcements","volume":" ","pages":"e0102524"},"PeriodicalIF":0.7,"publicationDate":"2025-02-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11812409/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142951514","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2025-02-11Epub Date: 2025-01-15DOI: 10.1128/mra.01186-24
Alexey V Sviridov, Sergey V Tarlachkov, Dmitry O Epiktetov, Tatyana V Shushkova, Marina P Petrakova, Alexey A Leontievsky
The genome of Ochrobactrum sp. isolated from agricultural soil polluted with the herbicide glyphosate is reported. The genome size is 5.48 Mb with an average G + C content of 56.3%. This genomic data could contribute to a better understanding of biochemistry and regulatory mechanisms of microbial degradation of glyphosate and 2-aminoethylphosphonic acid.
报道了从草甘膦污染的农业土壤中分离到的Ochrobactrum sp.的基因组。基因组大小为5.48 Mb, G + C平均含量为56.3%。这些基因组数据有助于更好地理解微生物降解草甘膦和2-氨基乙基膦酸的生物化学和调控机制。
{"title":"Draft genome sequence of glyphosate-degrading <i>Ochrobactrum</i> sp. strain GPK 3 (VKM B-2554D) isolated from agricultural soil.","authors":"Alexey V Sviridov, Sergey V Tarlachkov, Dmitry O Epiktetov, Tatyana V Shushkova, Marina P Petrakova, Alexey A Leontievsky","doi":"10.1128/mra.01186-24","DOIUrl":"10.1128/mra.01186-24","url":null,"abstract":"<p><p>The genome of <i>Ochrobactrum</i> sp. isolated from agricultural soil polluted with the herbicide glyphosate is reported. The genome size is 5.48 Mb with an average G + C content of 56.3%. This genomic data could contribute to a better understanding of biochemistry and regulatory mechanisms of microbial degradation of glyphosate and 2-aminoethylphosphonic acid.</p>","PeriodicalId":18654,"journal":{"name":"Microbiology Resource Announcements","volume":" ","pages":"e0118624"},"PeriodicalIF":0.7,"publicationDate":"2025-02-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11812299/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142984079","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2025-02-11Epub Date: 2025-01-15DOI: 10.1128/mra.00997-24
Allison R Mason, Gregory Johnson, Joseph Krampen, Jennifer N T Nguyen, Marcy J Balunas, Patrick D Schloss
mpactR automates pre-processing of liquid chromatography-tandem mass spectrometry (LC-MS/MS) data from microbiological samples to correct mispicked peaks, resolve inter-sample variation in abundance across technical replicates, account for in-source ion fragmentation, and remove background noise to yield high-quality mass spectrometry features. The package is available through CRAN and GitHub.
{"title":"mpactR: an R adaptation of the metabolomics peak analysis computational tool (MPACT) for use in reproducible data analysis pipelines.","authors":"Allison R Mason, Gregory Johnson, Joseph Krampen, Jennifer N T Nguyen, Marcy J Balunas, Patrick D Schloss","doi":"10.1128/mra.00997-24","DOIUrl":"10.1128/mra.00997-24","url":null,"abstract":"<p><p>mpactR automates pre-processing of liquid chromatography-tandem mass spectrometry (LC-MS/MS) data from microbiological samples to correct mispicked peaks, resolve inter-sample variation in abundance across technical replicates, account for in-source ion fragmentation, and remove background noise to yield high-quality mass spectrometry features. The package is available through CRAN and GitHub.</p>","PeriodicalId":18654,"journal":{"name":"Microbiology Resource Announcements","volume":" ","pages":"e0099724"},"PeriodicalIF":0.7,"publicationDate":"2025-02-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11812337/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142984084","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2025-02-11Epub Date: 2024-12-12DOI: 10.1128/mra.01097-24
Gordon Webster, Alex J Mullins, Eshwar Mahenthiralingam
Here, we report the genome sequence of Burkholderia ambifaria BCC0191, a biopesticide originally isolated from the barley rhizosphere. The genome was assembled using an Illumina-Nanopore hybrid approach and consisted of 7.62 Mbp distributed across three replicons. Several specialized metabolite biosynthetic gene clusters, including those known to be active in biocontrol, were identified.
{"title":"Complete genome sequence of the biopesticidal <i>Burkholderia ambifaria</i> strain BCC0191.","authors":"Gordon Webster, Alex J Mullins, Eshwar Mahenthiralingam","doi":"10.1128/mra.01097-24","DOIUrl":"10.1128/mra.01097-24","url":null,"abstract":"<p><p>Here, we report the genome sequence of <i>Burkholderia ambifaria</i> BCC0191, a biopesticide originally isolated from the barley rhizosphere. The genome was assembled using an Illumina-Nanopore hybrid approach and consisted of 7.62 Mbp distributed across three replicons. Several specialized metabolite biosynthetic gene clusters, including those known to be active in biocontrol, were identified.</p>","PeriodicalId":18654,"journal":{"name":"Microbiology Resource Announcements","volume":" ","pages":"e0109724"},"PeriodicalIF":0.7,"publicationDate":"2025-02-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11812388/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142813877","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Leptolyngbya phage Dor1 was induced by mitomycin C from a fishpond and was isolated on Leptolyngbya boryana IU 594. The 41,522-bp genome of Leptolyngbya phage Dor1 has 93.77% intergenomic similarity with Leptolyngbya phage LPP-1; however, unlike LPP-1, Dor1 carries an HNH endonuclease in its DNA polymerase gene.
{"title":"Genome sequence of Leptolyngbya phage Dor1, a cyanophage induced from a fish pond.","authors":"Esther Cattan-Tsaushu, Lilach Pessen, Sarit Avrani","doi":"10.1128/mra.01098-24","DOIUrl":"10.1128/mra.01098-24","url":null,"abstract":"<p><p>Leptolyngbya phage Dor1 was induced by mitomycin C from a fishpond and was isolated on <i>Leptolyngbya boryana</i> IU 594. The 41,522-bp genome of Leptolyngbya phage Dor1 has 93.77% intergenomic similarity with Leptolyngbya phage LPP-1; however, unlike LPP-1, Dor1 carries an HNH endonuclease in its DNA polymerase gene.</p>","PeriodicalId":18654,"journal":{"name":"Microbiology Resource Announcements","volume":" ","pages":"e0109824"},"PeriodicalIF":0.7,"publicationDate":"2025-02-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11812291/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142869624","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2025-02-11Epub Date: 2025-01-08DOI: 10.1128/mra.01090-24
Olga Andrievskaia, Amalia Garceac, Dara Lloyd, Mirjana Savic, Marc-Olivier Duceppe
Bovine tuberculosis is an important zoonotic infectious disease that presents a risk to human health, livestock, and wildlife. We report the complete genome sequence of a new Mycobacterium bovis strain that caused a bovine tuberculosis outbreak on a cattle farm in Saskatchewan, Canada, in 2023.
{"title":"Complete genome sequence of a <i>Mycobacterium bovis</i> strain associated with a bovine tuberculosis outbreak on a cattle farm in Saskatchewan, Canada.","authors":"Olga Andrievskaia, Amalia Garceac, Dara Lloyd, Mirjana Savic, Marc-Olivier Duceppe","doi":"10.1128/mra.01090-24","DOIUrl":"10.1128/mra.01090-24","url":null,"abstract":"<p><p>Bovine tuberculosis is an important zoonotic infectious disease that presents a risk to human health, livestock, and wildlife. We report the complete genome sequence of a new <i>Mycobacterium bovis</i> strain that caused a bovine tuberculosis outbreak on a cattle farm in Saskatchewan, Canada, in 2023.</p>","PeriodicalId":18654,"journal":{"name":"Microbiology Resource Announcements","volume":" ","pages":"e0109024"},"PeriodicalIF":0.7,"publicationDate":"2025-02-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11812355/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142951420","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2025-02-11Epub Date: 2025-01-08DOI: 10.1128/mra.01164-24
Andrew Montecillo, Jimwel Bryan Christopher Ferrer, Zyne Baybay, Ruffa Maze Balmes, Roselle Falconite-Cudal, Mary Grace Alba, Karl Alexander Fabros, Timothy James Dela Paz, Lucille C Villegas, Wreahlen Cariaso, Homer Pantua
We report two coding-complete genome sequences of porcine reproductive and respiratory syndrome virus 2 from field clinical samples obtained in 2021 (BA2021012A) and 2023 (ME20230008B-2) from the Philippines. BA2021012A (15,388 bp) is classified as a lineage L8C strain while ME20230008B-2 (15,513 bp) is a vaccine-like strain in lineage L7.
{"title":"Two coding-complete genomes of porcine reproductive and respiratory syndrome virus 2 (PRRSV-2) from field clinical samples in the Philippines.","authors":"Andrew Montecillo, Jimwel Bryan Christopher Ferrer, Zyne Baybay, Ruffa Maze Balmes, Roselle Falconite-Cudal, Mary Grace Alba, Karl Alexander Fabros, Timothy James Dela Paz, Lucille C Villegas, Wreahlen Cariaso, Homer Pantua","doi":"10.1128/mra.01164-24","DOIUrl":"10.1128/mra.01164-24","url":null,"abstract":"<p><p>We report two coding-complete genome sequences of porcine reproductive and respiratory syndrome virus 2 from field clinical samples obtained in 2021 (BA2021012A) and 2023 (ME20230008B-2) from the Philippines. BA2021012A (15,388 bp) is classified as a lineage L8C strain while ME20230008B-2 (15,513 bp) is a vaccine-like strain in lineage L7.</p>","PeriodicalId":18654,"journal":{"name":"Microbiology Resource Announcements","volume":" ","pages":"e0116424"},"PeriodicalIF":0.7,"publicationDate":"2025-02-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11812408/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142951519","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2025-02-11Epub Date: 2025-01-13DOI: 10.1128/mra.01228-24
Peter Kuhnert, Isabelle Brodard, Joerg Jores
The zoonotic pathogen Capnocytophaga canimorsus is part of the oral microbiome of dogs and cats. We report the genome sequence of strain KM3195_24, isolated from a red fox. It has a circular genome of 2,718,701 bp with a G + C content of 36%, 2,419 CDS, 3 rRNA operons, and 45 tRNAs.
{"title":"Complete genome sequence of the first <i>Capnocytophaga canimorus</i> strain isolated from a red fox (<i>Vulpes vulpes</i>) in Germany.","authors":"Peter Kuhnert, Isabelle Brodard, Joerg Jores","doi":"10.1128/mra.01228-24","DOIUrl":"10.1128/mra.01228-24","url":null,"abstract":"<p><p>The zoonotic pathogen <i>Capnocytophaga canimorsus</i> is part of the oral microbiome of dogs and cats. We report the genome sequence of strain KM3195_24, isolated from a red fox. It has a circular genome of 2,718,701 bp with a G + C content of 36%, 2,419 CDS, 3 rRNA operons, and 45 tRNAs.</p>","PeriodicalId":18654,"journal":{"name":"Microbiology Resource Announcements","volume":" ","pages":"e0122824"},"PeriodicalIF":0.7,"publicationDate":"2025-02-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11812287/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142971632","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2025-02-11Epub Date: 2025-01-13DOI: 10.1128/mra.00956-24
Sunjida Afrin, Michael Netherland, Halima Habib, Nur Hasan, Sangita Ahmed
We present the genome of Listeria monocytogenes BDSA isolated from ready-to-eat (RTE) meat collected in Dhaka, Bangladesh. The genome displays the Listeria pathogenicity island 1 and virulence, stress response, and antimicrobial resistance genes. It was phylogenetically classified as ST7, and clustered with serotype 1/2a belonging to lineage II.
{"title":"Draft genome sequence of <i>Listeria monocytogenes</i> BDSA strain isolated from ready-to-eat chicken product in Dhaka, Bangladesh.","authors":"Sunjida Afrin, Michael Netherland, Halima Habib, Nur Hasan, Sangita Ahmed","doi":"10.1128/mra.00956-24","DOIUrl":"10.1128/mra.00956-24","url":null,"abstract":"<p><p>We present the genome of <i>Listeria monocytogenes</i> BDSA isolated from ready-to-eat (RTE) meat collected in Dhaka, Bangladesh. The genome displays the Listeria pathogenicity island 1 and virulence, stress response, and antimicrobial resistance genes. It was phylogenetically classified as ST7, and clustered with serotype 1/2a belonging to lineage II.</p>","PeriodicalId":18654,"journal":{"name":"Microbiology Resource Announcements","volume":" ","pages":"e0095624"},"PeriodicalIF":0.7,"publicationDate":"2025-02-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11812353/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142971633","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}