With the uncertainty of COVID-19 disease around the world, the discovery and development of novel treatments for COVID-19 becoming an emerging trend. Network pharmacology has been used for determining the potential targets from several diseases. This research mainly focused on the potential of Allium schoenoprasum against COVID-19 based on a network pharmacology approach. The methods consist of target identification of the compounds, target identification related to COVID-19 disease, compound-target interaction network, protein-protein interaction network and gene ontology and pathway enrichment analysis. Fifthy three main targets obtained from the compound-COVID-19 were identified as main targets from the compounds with MMP9, MPO, TLR4, MMP2, CCNB1, AURKB, PLK1, TOP2A, ALOX5, and CD38 becoming the top 10 core targets. Phenolic compounds in Allium schoenoprasum may act as anti-COVID-19 through several inflammatory and immune response pathways. Based on these results, it seems that phenolic compounds in Allium schoenoprasum might act as anti-COVID-19 via network pharmacology approaches.
{"title":"Identification of Potential COVID-19 Targets and Pathways Derivate from Various Phenolic Compounds from Chives (Allium schoenoprasum) by Using Network Pharmacology Approach","authors":"Iksen, B. Buana","doi":"10.24123/mpi.v4i2.5272","DOIUrl":"https://doi.org/10.24123/mpi.v4i2.5272","url":null,"abstract":"\u0000 \u0000 \u0000 \u0000With the uncertainty of COVID-19 disease around the world, the discovery and development of novel treatments for COVID-19 becoming an emerging trend. Network pharmacology has been used for determining the potential targets from several diseases. This research mainly focused on the potential of Allium schoenoprasum against COVID-19 based on a network pharmacology approach. The methods consist of target identification of the compounds, target identification related to COVID-19 disease, compound-target interaction network, protein-protein interaction network and gene ontology and pathway enrichment analysis. Fifthy three main targets obtained from the compound-COVID-19 were identified as main targets from the compounds with MMP9, MPO, TLR4, MMP2, CCNB1, AURKB, PLK1, TOP2A, ALOX5, and CD38 becoming the top 10 core targets. Phenolic compounds in Allium schoenoprasum may act as anti-COVID-19 through several inflammatory and immune response pathways. Based on these results, it seems that phenolic compounds in Allium schoenoprasum might act as anti-COVID-19 via network pharmacology approaches. \u0000 \u0000 \u0000 \u0000","PeriodicalId":18807,"journal":{"name":"MPI (Media Pharmaceutica Indonesiana)","volume":"28 10","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-12-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"72569351","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Corona virus disease 2019 caused by SARS-CoV-2 infection emerged in late 2019 and still become a worldwide pandemic up to this point with the drug remain unavailable. Meanwhile, Indonesia has an abundance variety of medicinal plants that are potential to be developed as inhibitors. By using the key role proteins as drug targets, namely spike glycoprotein and RNA-dependent RNA polymerase (RdRp) of delta variant of SARS-CoV-2 (which is known as strongly transmitted and highly virulent), we can develop inhibitors for the target proteins from potential Indonesian medicinal plants to prevent the protein interactions for viral entry and proliferation that leading to organ disfunction and death. This study aimed to identify the secondary metabolites of various Indonesian medicinal plants as SARS-CoV-2 inhibitors. The 184 ligands from nine plants were collected from IJAH webserver and their SMILES notation were collected from PubChem. Meanwhile 3D structures of spike glycoprotein (PDB ID: 6VXX) and RdRp (PDB ID: 6M71) were obtained from protein data bank (PDB). Molecular docking was conducted between ligands and the two SARS-CoV-2 proteins using Autodock Vina in PyRx with hesperidin and remdesivir as control compounds. Several potential compounds were selected for drug-likeness analysis and toxicity analysis. Results showed that lantanolic acid has the same amino acid interaction with RdRp as the control compound. It formed a hydrogen bond with Ser784 and hydrophobic bonds with Tyr32 and Ser7709. It had lower binding affinity than the control compounds, eligible as oral drug, and had LD50 of 2589 mg/kg.
{"title":"Secondary Metabolites of Various Indonesian Medicinal Plants as SARS-CoV-2 Inhibitors: In Silico Study","authors":"Emilia Tungary, M. Wahjudi, Tjie Kok","doi":"10.24123/mpi.v4i2.5255","DOIUrl":"https://doi.org/10.24123/mpi.v4i2.5255","url":null,"abstract":"\u0000 \u0000 \u0000 \u0000Corona virus disease 2019 caused by SARS-CoV-2 infection emerged in late 2019 and still become a worldwide pandemic up to this point with the drug remain unavailable. Meanwhile, Indonesia has an abundance variety of medicinal plants that are potential to be developed as inhibitors. By using the key role proteins as drug targets, namely spike glycoprotein and RNA-dependent RNA polymerase (RdRp) of delta variant of SARS-CoV-2 (which is known as strongly transmitted and highly virulent), we can develop inhibitors for the target proteins from potential Indonesian medicinal plants to prevent the protein interactions for viral entry and proliferation that leading to organ disfunction and death. This study aimed to identify the secondary metabolites of various Indonesian medicinal plants as SARS-CoV-2 inhibitors. The 184 ligands from nine plants were collected from IJAH webserver and their SMILES notation were collected from PubChem. Meanwhile 3D structures of spike glycoprotein (PDB ID: 6VXX) and RdRp (PDB ID: 6M71) were obtained from protein data bank (PDB). Molecular docking was conducted between ligands and the two SARS-CoV-2 proteins using Autodock Vina in PyRx with hesperidin and remdesivir as control compounds. Several potential compounds were selected for drug-likeness analysis and toxicity analysis. Results showed that lantanolic acid has the same amino acid interaction with RdRp as the control compound. It formed a hydrogen bond with Ser784 and hydrophobic bonds with Tyr32 and Ser7709. It had lower binding affinity than the control compounds, eligible as oral drug, and had LD50 of 2589 mg/kg. \u0000 \u0000 \u0000 \u0000","PeriodicalId":18807,"journal":{"name":"MPI (Media Pharmaceutica Indonesiana)","volume":"341 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-12-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"87790678","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Arhadianto Adi Nugroho, F. Christina, G. Buschle-Diller, M. Purwanto, Christina Mumpuni Erawati, Ardhia Deasy Rosita Dewi, Y. P. Mukti, J. Sukweenadhi
Water kefir is a fermented carbonated beverage and functional food with probiotic properties. Broccoli is an edible green plant which a rich source of vitamins A, B1, B2, B3, and C; it also contains isothiocyanate compounds that have anti-cancer and fiber for digestive system. Palm sugar is a sweetener obtained from concentrated coconut sap and is known to have a low glycaemic index. The purpose of this study was to investigate the effect of broccoli stem extract and palm sugar concentration on its biochemical and microbiological characteristics. Subsequently, the acceptance of the product by the panelists were evaluated through organoleptic tests. Water kefir with sugar content of 15% had the highest lactic acid, antioxidant activity, lactic acid bacteria, yeast, ethanol content, and water kefir with a concentration of 1:5 and sugar content of 15% found the highest acceptance by the panelists. Water kefir obtained in this study did not have contaminant bacteria. This product also met the codex standard.
{"title":"Characterization of Water Kefir from Broccoli Stem Extract with Addition of Palm Sugar","authors":"Arhadianto Adi Nugroho, F. Christina, G. Buschle-Diller, M. Purwanto, Christina Mumpuni Erawati, Ardhia Deasy Rosita Dewi, Y. P. Mukti, J. Sukweenadhi","doi":"10.24123/mpi.v4i2.5259","DOIUrl":"https://doi.org/10.24123/mpi.v4i2.5259","url":null,"abstract":"\u0000 \u0000 \u0000 \u0000Water kefir is a fermented carbonated beverage and functional food with probiotic properties. Broccoli is an edible green plant which a rich source of vitamins A, B1, B2, B3, and C; it also contains isothiocyanate compounds that have anti-cancer and fiber for digestive system. Palm sugar is a sweetener obtained from concentrated coconut sap and is known to have a low glycaemic index. The purpose of this study was to investigate the effect of broccoli stem extract and palm sugar concentration on its biochemical and microbiological characteristics. Subsequently, the acceptance of the product by the panelists were evaluated through organoleptic tests. Water kefir with sugar content of 15% had the highest lactic acid, antioxidant activity, lactic acid bacteria, yeast, ethanol content, and water kefir with a concentration of 1:5 and sugar content of 15% found the highest acceptance by the panelists. Water kefir obtained in this study did not have contaminant bacteria. This product also met the codex standard. \u0000 \u0000 \u0000 \u0000","PeriodicalId":18807,"journal":{"name":"MPI (Media Pharmaceutica Indonesiana)","volume":"114 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-12-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"80943273","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Febrina Amelia Saputri, Abdul Mun’im, Chinthia Rahadi Putri, Dewi Aryani
Rimpang temulawak (Curcuma xanthorrhiza) merupakan bagian tanaman yang banyak dimanfaatkan untuk pengobatan tradisional. Metabolit sekunder yang memberikan aktivitas farmakologisnya adalah senyawa kurkuminoid dan xantorizol. Penelitian ini bertujuan untuk memperoleh metode analisis kurkuminoid dan xantorizol yang tervalidasi, cepat, dan sederhana. Analisis dilakukan dengan menggunakan fase diam berupa lempeng KLT silika gel 60 GF254 dan fase gerak diklorometana-kloroform (4:6). Pemindaian (scanning) densitometrik dilakukan pada 224 nm untuk xantorizol dan 425 nm untuk kurkuminoid. Metode menunjukan linieritas, akurasi, dan presisi yang baik, dengan batas deteksi kurkuminoid dan xantorizol yaitu 7,88 bpj dan 28,23 bpj dan batas kuantitasi kurkuminoid dan xantorizol yaitu 23,89 bpj dan 85,55 bpj. Kadar kurkuminoid dan xantorizol pada ekstrak rimpang temulawak adalah 4,95 ± 0,01 dan 31,30 ± 0,09 mg/g serbuk simplisia. The rhizome of temulawak (Curcuma xanthorrhiza) is a part of the plant that is widely used for traditional medicine. Secondary metabolites that provide pharmacological activity are curcuminoids and xanthorrhizol. This study aims to obtain a validated, fast, and simple analytical method for curcuminoids and xanthorrhizol. The analysis was carried out with the stationary phase TLC plates silica gel 60 GF254. The mobile phase used was dichloromethane-chloroform (4:6), with densitometric scanning at 224 nm for xanthorrhizol and 425 nm for curcuminoids. The method showed good linearity, accuracy, and precision, with curcuminoids and xanthorrhizol detection limits of 7.88 ppm and 28.23 ppm, curcuminoids and xanthorrhizol quantitation limits of 23.89 ppm and 85.55 ppm, respectively. The content of curcuminoids and xanthorrhizol in temulawak rhizome extract were 4.95 ± 0.01 and 31.30 ± 0.09 mg/g Simplicia powder, respectively.
{"title":"Validasi Metode Analisis Kurkuminoid dan Xantorizol pada Rimpang Temulawak (Curcuma xanthorrhiza) dengan KLT- Densitometri","authors":"Febrina Amelia Saputri, Abdul Mun’im, Chinthia Rahadi Putri, Dewi Aryani","doi":"10.24123/mpi.v4i2.5297","DOIUrl":"https://doi.org/10.24123/mpi.v4i2.5297","url":null,"abstract":"\u0000 \u0000 \u0000 \u0000 \u0000 \u0000 \u0000 \u0000Rimpang temulawak (Curcuma xanthorrhiza) merupakan bagian tanaman yang banyak dimanfaatkan untuk pengobatan tradisional. Metabolit sekunder yang memberikan aktivitas farmakologisnya adalah senyawa kurkuminoid dan xantorizol. Penelitian ini bertujuan untuk memperoleh metode analisis kurkuminoid dan xantorizol yang tervalidasi, cepat, dan sederhana. Analisis dilakukan dengan menggunakan fase diam berupa lempeng KLT silika gel 60 GF254 dan fase gerak diklorometana-kloroform (4:6). Pemindaian (scanning) densitometrik dilakukan pada 224 nm untuk xantorizol dan 425 nm untuk kurkuminoid. Metode menunjukan linieritas, akurasi, dan presisi yang baik, dengan batas deteksi kurkuminoid dan xantorizol yaitu 7,88 bpj dan 28,23 bpj dan batas kuantitasi kurkuminoid dan xantorizol yaitu 23,89 bpj dan 85,55 bpj. Kadar kurkuminoid dan xantorizol pada ekstrak rimpang temulawak adalah 4,95 ± 0,01 dan 31,30 ± 0,09 mg/g serbuk simplisia. \u0000 \u0000 \u0000 \u0000 \u0000 \u0000 \u0000 \u0000 \u0000 \u0000 \u0000 \u0000 \u0000 \u0000The rhizome of temulawak (Curcuma xanthorrhiza) is a part of the plant that is widely used for traditional medicine. Secondary metabolites that provide pharmacological activity are curcuminoids and xanthorrhizol. This study aims to obtain a validated, fast, and simple analytical method for curcuminoids and xanthorrhizol. The analysis was carried out with the stationary phase TLC plates silica gel 60 GF254. The mobile phase used was dichloromethane-chloroform (4:6), with densitometric scanning at 224 nm for xanthorrhizol and 425 nm for curcuminoids. The method showed good linearity, accuracy, and precision, with curcuminoids and xanthorrhizol detection limits of 7.88 ppm and 28.23 ppm, curcuminoids and xanthorrhizol quantitation limits of 23.89 ppm and 85.55 ppm, respectively. The content of curcuminoids and xanthorrhizol in temulawak rhizome extract were 4.95 ± 0.01 and 31.30 ± 0.09 mg/g Simplicia powder, respectively. \u0000 \u0000 \u0000 \u0000 \u0000 \u0000 \u0000 \u0000 \u0000 \u0000 \u0000 \u0000","PeriodicalId":18807,"journal":{"name":"MPI (Media Pharmaceutica Indonesiana)","volume":"22 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-12-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"84870087","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Aguslina Kirtishanti, Dini Kesuma, Fadita Trisa C, Maria Claudia Dwiyanti Tuga
Kanker payudara dan servik merupakan kanker dengan jumlah terbanyak di Indonesia. Kemoterapi sebagai terapi kanker memiliki banyak efek samping, oleh karena itu diperlukan pengembangan obat antikanker terutama dari bahan alam yang efektif dan memiliki efek samping minimal. Salah satu bahan alam yang diprediksi mempunyai aktivitas antikanker adalah biji alpukat (Persea americana Mill.). Penelitian ini bertujuan untuk menentukan aktivitas sitotoksik ekstrak etanol biji alpukat pada sel kanker payudara dan serviks secara in silico dan in vitro. Senyawa aktif dalam biji alpukat di docking dengan reseptor estrogen (PDB code: 3ERT) and reseptor SIRT1 (PDB code: 4I5I) menggunakan program Molegro Virtual Docker 5.5 (MVD). Aktivitas sitotoksik secara in vitro dilakukan menggunakan metode Microculture Tetrazolium Technique (MTT) pada sel kanker payudara (MCF7), sel kanker serviks (HeLa) dan sel normal (Vero). Biji alpukat berisi 10 senyawa aktif yang diprediksi mempunyai aktivitas sitotoksik. Hasil uji in silico menunjukkan bahwa epicatechin gallate mempunyai nilai rerank score paling rendah yaitu -118,397 kkal/mol pada reseptor estrogen dan -133,694 kkal/mol pada reseptor SIRT1. Aktivitas sitotoksik secara in vitro ditunjukkan dengan nilai IC50 sebesar 537,37 μg/mL (MCF7), 383,21 μg/mL (HeLa) dan 541,67 μg/mL (Vero). Dari hasil uji in vitro menyatakan bahwa ekstrak etanol biji alpukat tidak memiliki aktivitas sitotoksik pada sel kanker MCF7 dan memiliki aktivitas sitotoksik lemah pada sel HeLa. Breast and cervical cancer are cancers with the highest number in Indonesia. Chemotherapy, as one of the mainstay treatments of cancer, can cause harmful side effects; and, therefore, it is necessary to develop anticancer drug from natural ingredients with good efficacy and minimal side effects. One of the natural ingredients that is predicted to have anticancer activity is avocado seed (Persea americana Mill.). This study aimed to evaluate the in-vitro and in-silico cytotoxic activity of avocado seed extract on breast and cervical cancer cells. The active compounds in avocado seeds were docked with estrogen receptors (PDB code: 3ERT) and SIRT1 receptors (PDB code: 4I5I) using the MVD program. Cytotoxic activity in vitro was carried out using the MTT method on breast cancer cells (MCF7), cervical cancer cells (HeLa) and normal cells (Vero). Avocado seed contains 10 active compounds which are predicted to have cytotoxic activity. The findings from in-silico test showed that the “epicatechin gallate” had the lowest rerank score, i.e. -118.397 kcal/mol for the estrogen receptor and -133.694 kcal/ mol for the SIRT1 receptor. Cytotoxic activity in vitro was shown by IC50 values of 537.37 μg/mL (MCF7), 383.21 μg/mL (HeLa) and 541.67 μg/mL (Vero), respectively. The findings from in-vitro test showed that the avocado seed extract did not have cytotoxic activity on MCF7 cells and had weak cytotoxic activity on HeLa cells.
乳腺癌和宫颈癌是印尼癌症数量最多的国家之一。化疗作为癌症治疗有很多副作用，所以它需要开发抗癌症药物，尤其是有效的天然成分，并产生最小的副作用。预测的天然抗癌活性之一是鳄梨籽。这项研究的目的是确定乳腺癌和二氧化硅子宫颈的卵巢癌乙醇乙醇的活性。在鳄梨种子中与雌激素感受器(gdp code: 3ERT)和SIRT1受体(gdp code: 4I5I)的活性化合物使用了虚拟文档5.5 (MVD)程序。细胞毒性在体外活动是使用乳腺癌(MCF7)、宫颈癌(HeLa)和正常细胞(Vero)的微文化四氯化技术(MTT)进行的。鳄梨籽含有10种可预测的活性化合物，其毒性很强。硅二醇的测试结果显示，epicatechin gallate的平均得分为-118,397 kkal/mol在雌激素受体和-133,694 kkal/mol在SIRT1受体。sitotoksik活动表现出体外地价值大小的螺旋藻537.37μg / mL (MCF7), 383.21μg / mL (HeLa)和541.67μg / mL(维罗)。根据体外试验的结果，鳄梨籽乙醇的提取物在MCF7癌细胞中没有细胞毒性，在HeLa细胞中有较弱的细胞毒性。欧洲乳腺和欧洲巨蟹座是印尼最顶尖的癌症患者。化疗，作为巨蟹座的主要治疗之一，可以造成伤害的一面影响;因此，必须有效地开发抗癌症药物，使其具有良好的效率和最低的副作用。一种天然的活性成分是鳄梨籽。这项研究允许评估内部vitro和二氧化硅硅藻土行为。在鳄梨种子中活跃的化合物使用MVD程序与雌激素检索器(gdp代码:3ERT)和SIRT1 receptors (gdp规范:4I5I)结合在一起。体外受精现象被考虑使用欧洲乳腺癌(MCF7)、欧洲癌症和正常病例的MTT方法。鳄梨种子接触10种活性化合物，这是预定的细胞毒性活动。来自西利科测试的最终结果显示，“epicatechin gallate”的“epicatechin gallate”的重复分数较低，i - 118,397 kcal/mol for estrogen receptor和- 133,694 kcal/mol for SIRT1 receptor。体外Cytotoxic活动是展示:螺旋藻的价值观。37μg / mL (MCF7 537), 383。21μg / mL (HeLa)和第541。67μg / mL(型号),respectively。最后的体外试验表明，鳄梨籽extract在MCF7细胞中没有细胞毒性反应，肝脏也没有发育不良。
{"title":"Aktivitas Sitotoksik Ekstrak Biji Alpukat (Persea americana Mill.) pada Sel Kanker Payudara dan Serviks Secara In Silico dan In Vitro","authors":"Aguslina Kirtishanti, Dini Kesuma, Fadita Trisa C, Maria Claudia Dwiyanti Tuga","doi":"10.24123/mpi.v4i2.5296","DOIUrl":"https://doi.org/10.24123/mpi.v4i2.5296","url":null,"abstract":"\u0000 \u0000 \u0000 \u0000Kanker payudara dan servik merupakan kanker dengan jumlah terbanyak di Indonesia. Kemoterapi sebagai terapi kanker memiliki banyak efek samping, oleh karena itu diperlukan pengembangan obat antikanker terutama dari bahan alam yang efektif dan memiliki efek samping minimal. Salah satu bahan alam yang diprediksi mempunyai aktivitas antikanker adalah biji alpukat (Persea americana Mill.). Penelitian ini bertujuan untuk menentukan aktivitas sitotoksik ekstrak etanol biji alpukat pada sel kanker payudara dan serviks secara in silico dan in vitro. Senyawa aktif dalam biji alpukat di docking dengan reseptor estrogen (PDB code: 3ERT) and reseptor SIRT1 (PDB code: 4I5I) menggunakan program Molegro Virtual Docker 5.5 (MVD). Aktivitas sitotoksik secara in vitro dilakukan menggunakan metode Microculture Tetrazolium Technique (MTT) pada sel kanker payudara (MCF7), sel kanker serviks (HeLa) dan sel normal (Vero). Biji alpukat berisi 10 senyawa aktif yang diprediksi mempunyai aktivitas sitotoksik. Hasil uji in silico menunjukkan bahwa epicatechin gallate mempunyai nilai rerank score paling rendah yaitu -118,397 kkal/mol pada reseptor estrogen dan -133,694 kkal/mol pada reseptor SIRT1. Aktivitas sitotoksik secara in vitro ditunjukkan dengan nilai IC50 sebesar 537,37 μg/mL (MCF7), 383,21 μg/mL (HeLa) dan 541,67 μg/mL (Vero). Dari hasil uji in vitro menyatakan bahwa ekstrak etanol biji alpukat tidak memiliki aktivitas sitotoksik pada sel kanker MCF7 dan memiliki aktivitas sitotoksik lemah pada sel HeLa. \u0000 \u0000 \u0000 \u0000 \u0000 \u0000 \u0000 \u0000 \u0000 \u0000Breast and cervical cancer are cancers with the highest number in Indonesia. Chemotherapy, as one of the mainstay treatments of cancer, can cause harmful side effects; and, therefore, it is necessary to develop anticancer drug from natural ingredients with good efficacy and minimal side effects. One of the natural ingredients that is predicted to have anticancer activity is avocado seed (Persea americana Mill.). This study aimed to evaluate the in-vitro and in-silico cytotoxic activity of avocado seed extract on breast and cervical cancer cells. The active compounds in avocado seeds were docked with estrogen receptors (PDB code: 3ERT) and SIRT1 receptors (PDB code: 4I5I) using the MVD program. Cytotoxic activity in vitro was carried out using the MTT method on breast cancer cells (MCF7), cervical cancer cells (HeLa) and normal cells (Vero). Avocado seed contains 10 active compounds which are predicted to have cytotoxic activity. The findings from in-silico test showed that the “epicatechin gallate” had the lowest rerank score, i.e. -118.397 kcal/mol for the estrogen receptor and -133.694 kcal/ mol for the SIRT1 receptor. Cytotoxic activity in vitro was shown by IC50 values of 537.37 μg/mL (MCF7), 383.21 μg/mL (HeLa) and 541.67 μg/mL (Vero), respectively. The findings from in-vitro test showed that the avocado seed extract did not have cytotoxic activity on MCF7 cells and had weak cytotoxic activity on HeLa cells. \u0000 \u0000 \u0000 \u0000","PeriodicalId":18807,"journal":{"name":"MPI (Media Pharmaceutica Indonesiana)","volume":"72 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-12-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"91066135","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
M. Rizki, Anna Khumaira Sari, Dewi Kartika, Amalia Khairunnisa, Normaidah
Daun cempedak (Artocarpus integer) dimanfaatkan secara tradisional oleh masyarakat Kalimantan Selatan dalam pengobatan. Golongan fenolik terkandung dalam ekstrak daun cempedak dan memiliki kemampuan sebagai antioksidan. Fraksi dari ekstrak daun cempedak belum pernah ditetapkan kadar fenolik total dan akivitas antioksidannya. Penelitian ini bertujuan untuk mengetahui fraksi dari ekstrak daun cempedak yang mengandung kadar fenolik dan aktivitas antioksidan tertinggi. Penelitian dimulai dengan pengeringan daun segar menggunakan lemari pengering, pembuatan serbuk simplisia, proses ekstraksi secara maserasi, pengeringan ekstrak dengan lemari pengering, dan proses fraksinasi dengan pelarut n-heksan, etil asetat dan aquadest. Penetapan kadar fenolik total dapat dilakukan dengan reagen Folin-Ciocalteu dan menggunakan baku pembanding asam galat. Aktivitas antioksidan dilakukan menggunakan metode DPPH berdasarkan nilai IC50 dan dengan pembanding kuersetin. Kadar fenolik total pada fraksi n-heksan 9,352 ± 0,113% b/b, fraksi etil asetat 12,595 ± 0,415% b/b, dan fraksi aquadest 9,992 ± 0,485% b/b ekivalen asam galat. Fraksi n-heksan, etil asetat, dan aquadest masing-masing memiliki nilai IC50 sebesar 89,192 ± 2,91 bpj (kuat), 64,754 ± 2,803 bpj (kuat), dan 82,247 ± 23,034 bpj (kuat). Kesimpulan dari penelitian ini bahwa fraksi etil asetat memiliki kadar fenolik total tertinggi dan aktivitas antioksidan paling kuat dibandingkan fraksi n-heksan dan fraksi aquadest dari ekstrak daun cempedak. Cempedak (Artocarpus integer) leaves are traditionally used by people in South Kalimantan as medicine. The phenolic group contained in cempedak leaf extract has known as an antioxidant. The fraction of cempedak leaf extract has never been determined for total phenolic content and antioxidant activity. This study aimed to determine the fraction of cempedak leaf extract that contains the highest levels of phenolic and antioxidant activity. The study started with drying of fresh leaves using a drying cabinet, simplicia powder preparation, maceration extraction, extract drying, and fractionation with n-hexane, ethyl acetate and aquadest as solvents. Determination of total phenolic content was obtained with Folin-Ciocalteu reagent and gallic acid as a standard for comparison. Antioxidant activity was measured using the DPPH method based on the IC50 value and comparison with quercetin. The total phenolic content of the n-hexane, ethyl acetate and aquadest fraction were 9.352 ± 0.113% w/w, ethyl acetate fraction 12.595 ± 0.415% w/w, and aquadest fraction 9.992 ± 0.485% w/w equivalent gallic acid, respectively. The n-hexane, ethyl acetate, and aquadest fractions showed IC50 values of 89.192 ± 2.91 ppm (strong), 64.754 ± 2.803 ppm (strong), and 82.247 ± 23.034 ppm (strong), respectively. The conclusion of this study was that the ethyl acetate fraction had highest total phenolic content and strongest antioxidant activity compared to n-hexane
红叶(阿托卡普integer)传统上被加里曼丹南部的人民用于治疗。酚类植物存在于淀粉提取物中，具有抗氧化剂的能力。无淀粉提取物的微量成分从未确定其总表现性和抗氧化酶酶。本研究旨在确定淀粉提取物中含有最高的酚含量和抗氧化剂活性的成分。研究开始于使用干燥柜、简单的花粉生成、成熟的提取过程、干燥柜的提取物干燥、盐乙醇和aquadest溶剂的点化和炼金术。总表型测定可与活体试剂folincioteu一起执行，并使用错误酸对照组。抗氧化剂活性使用基于IC50值和kuersetin的DPPH方法进行。总酚含量的派别n-heksan 9,352±0,113% b - b,醋酸纤维素乙醇成分12,595±0,415% b / b,派别aquadest 9,992±0,485% b / b错误ekivalen酸。n-heksan、醋酸纤维素乙醇成分和螺旋藻aquadest每个人都有自己的价值高达89.192±2,91草根阶层(强大),64.754±2,803草根阶层(强大),82.247±23,034草根阶层(强大)。这项研究得出的结论是，醋酸乙醇的总ph值和抗氧化物活性比蛋白酶提取物中的n-heksan和aquadest的提取物浓度最高。Cempedak (Artocarpus integer)的传统用法是由加里曼丹南部的人们共同使用的。被关在cempedak leaf extract的phenc group被称为反氧剂。cempedak leaf extract的股份从未确定为总油性和抗氧化剂行为。这项研究可以确定淀粉的成分暴露在酚和抗氧化剂行为的最上层。研究始于通过一个干树叶的柜子、simplicia火药的准备、大规模撤离、extract排水和与n-hexane、ethyl acetate和美国solvents的蒸馏。总phenolic的决定被死胎和gallic acid合并为标准的综合。反氧化剂活动是采用基于IC50值的DPPH方法并与quercetin进行比较的。n-hexane总酚内容》,乙基醋酸和aquadest fraction是9352±醋酸0.113% w / w,乙基fraction 12595±0.415% w / w,和aquadest fraction 9992±0.485% w / w,高卢哪里酸,respectively。《n-hexane、乙基醋酸和aquadest fractions那里螺旋藻的价值观89192±2 . 91 mtc(合编),64754±2.803 mtc(合编),和82247±23034 mtc(合编),respectively)。这项研究的结论是，ethyl acection的强度和抗氧化性行为与cempedak leaf extract的抗氧化性和抗氧化性行为的强度最高。
{"title":"Penetapan Kadar Fenolik Total dan Uji Aktivitas Antioksidan Fraksi dari Ekstrak Etanol Daun Cempedak (Artocarpus integer) dengan Metode DPPH","authors":"M. Rizki, Anna Khumaira Sari, Dewi Kartika, Amalia Khairunnisa, Normaidah","doi":"10.24123/mpi.v4i2.4937","DOIUrl":"https://doi.org/10.24123/mpi.v4i2.4937","url":null,"abstract":"\u0000 \u0000 \u0000 \u0000 \u0000 \u0000 \u0000 \u0000Daun cempedak (Artocarpus integer) dimanfaatkan secara tradisional oleh masyarakat Kalimantan Selatan dalam pengobatan. Golongan fenolik terkandung dalam ekstrak daun cempedak dan memiliki kemampuan sebagai antioksidan. Fraksi dari ekstrak daun cempedak belum pernah ditetapkan kadar fenolik total dan akivitas antioksidannya. Penelitian ini bertujuan untuk mengetahui fraksi dari ekstrak daun cempedak yang mengandung kadar fenolik dan aktivitas antioksidan tertinggi. Penelitian dimulai dengan pengeringan daun segar menggunakan lemari pengering, pembuatan serbuk simplisia, proses ekstraksi secara maserasi, pengeringan ekstrak dengan lemari pengering, dan proses fraksinasi dengan pelarut n-heksan, etil asetat dan aquadest. Penetapan kadar fenolik total dapat dilakukan dengan reagen Folin-Ciocalteu dan menggunakan baku pembanding asam galat. Aktivitas antioksidan dilakukan menggunakan metode DPPH berdasarkan nilai IC50 dan dengan pembanding kuersetin. Kadar fenolik total pada fraksi n-heksan 9,352 ± 0,113% b/b, fraksi etil asetat 12,595 ± 0,415% b/b, dan fraksi aquadest 9,992 ± 0,485% b/b ekivalen asam galat. Fraksi n-heksan, etil asetat, dan aquadest masing-masing memiliki nilai IC50 sebesar 89,192 ± 2,91 bpj (kuat), 64,754 ± 2,803 bpj (kuat), dan 82,247 ± 23,034 bpj (kuat). Kesimpulan dari penelitian ini bahwa fraksi etil asetat memiliki kadar fenolik total tertinggi dan aktivitas antioksidan paling kuat dibandingkan fraksi n-heksan dan fraksi aquadest dari ekstrak daun cempedak. \u0000 \u0000 \u0000 \u0000 \u0000 \u0000 \u0000 \u0000 \u0000 \u0000 \u0000 \u0000 \u0000 \u0000Cempedak (Artocarpus integer) leaves are traditionally used by people in South Kalimantan as medicine. The phenolic group contained in cempedak leaf extract has known as an antioxidant. The fraction of cempedak leaf extract has never been determined for total phenolic content and antioxidant activity. This study aimed to determine the fraction of cempedak leaf extract that contains the highest levels of phenolic and antioxidant activity. The study started with drying of fresh leaves using a drying cabinet, simplicia powder preparation, maceration extraction, extract drying, and fractionation with n-hexane, ethyl acetate and aquadest as solvents. Determination of total phenolic content was obtained with Folin-Ciocalteu reagent and gallic acid as a standard for comparison. Antioxidant activity was measured using the DPPH method based on the IC50 value and comparison with quercetin. The total phenolic content of the n-hexane, ethyl acetate and aquadest fraction were 9.352 ± 0.113% w/w, ethyl acetate fraction 12.595 ± 0.415% w/w, and aquadest fraction 9.992 ± 0.485% w/w equivalent gallic acid, respectively. The n-hexane, ethyl acetate, and aquadest fractions showed IC50 values of 89.192 ± 2.91 ppm (strong), 64.754 ± 2.803 ppm (strong), and 82.247 ± 23.034 ppm (strong), respectively. The conclusion of this study was that the ethyl acetate fraction had highest total phenolic content and strongest antioxidant activity compared to n-hexane","PeriodicalId":18807,"journal":{"name":"MPI (Media Pharmaceutica Indonesiana)","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-12-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"89046810","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Brevmana Anugrah Primulyanto, Yosi Irawati Wibowo, E. Setiawan, Cecilia Brata
Dispepsia merupakan salah satu gejala yang umum dijumpai di apotek, dan oleh karena itu apoteker perlu untuk mampu memberikan rekomendasi yang tepat pada pasien dengan gejala dispepsia. Penelitian ini bertujuan untuk mendeskripsikan tipe dan ketepatan rekomendasi pada dua kasus vignette dispepsia. Penelitian ini menggunakan desain potong lintang dan 42 apoteker berpartisipasi dalam penelitian ini. Instrumen penelitian menggunakan kuesioner yang berisi: (1) karakteristik apoteker dan apotek serta (2) dua kasus vignette: kasus dispepsia tanpa alarm symptoms dan kasus dispepsia karena Na diklofenak. Pengambilan data dilakukan dengan wawancara terstruktur. Analisis data dilakukan dengan inductive content analysis. Ketepatan rekomendasi dinilai berdasarkan literatur dan opini expert panel. Tipe rekomendasi yang paling sering diberikan oleh apoteker pada dua kasus di atas adalah memberikan produk untuk gejala dispepsia. Ketepatan rekomendasi pada kasus dispepsia tanpa alarm symptom dan pada kasus dispepsia karena Na diklofenak secara berturut-turut adalah 83% dan 21% dari 42 apoteker yang berpartisipasi. Dapat disimpulkan bahwa ketepatan rekomendasi bervariasi tergantung kasus. Perlu adanya peningkatan kemampuan apoteker dalam memberikan rekomendasi yang tepat, terutama pada kasus swamedikasi dispepsia yang membutuhkan rujukan ke dokter. Dyspepsia is a symptom commonly seen in community pharmacies, and therefore pharmacists need to be able to provide appropriate recommendations for patients with dyspepsia. This study aims to describe the types and the appropriateness of the recommendation provided by pharmacists when responding to two vignette cases of dyspepsia. This study was a cross sectional study, and 42 community pharmacists participated in the study. A questionnaire consisting of (1) pharmacists and pharmacy characteristics, and (2) two vignette cases of dyspepsia without alarm symptoms and dyspepsia due to Na diclofenac was developed. A structured interview was used for data collection, and an inductive content analysis was used to analyse the cases. The appropriateness of the recommendation was assessed based on the literature and expert panel opinion. The most common type of recommendation provided in the 2 vignette cases was recommending product for dyspepsia. Appropriate recommendation was provided by 83% and 21% of the 42 participating pharmacists in the case of dyspepsia without alarm symptoms and in the case of dyspepia due to Na diclofenac respectively. The appropriateness of the recommendation varied depending on the case. There is a need to improve the capability of pharmacists in providing appropriate recommendation, particularly for a dyspepsia case that needs medical referral.
{"title":"Profil Tipe dan Ketepatan Rekomendasi Apoteker pada Kasus Vignette Dispesia di Salah Satu Kabupaten di Jawa Timur Indonesia","authors":"Brevmana Anugrah Primulyanto, Yosi Irawati Wibowo, E. Setiawan, Cecilia Brata","doi":"10.24123/mpi.v4i2.5331","DOIUrl":"https://doi.org/10.24123/mpi.v4i2.5331","url":null,"abstract":"\u0000 \u0000 \u0000 \u0000Dispepsia merupakan salah satu gejala yang umum dijumpai di apotek, dan oleh karena itu apoteker perlu untuk mampu memberikan rekomendasi yang tepat pada pasien dengan gejala dispepsia. Penelitian ini bertujuan untuk mendeskripsikan tipe dan ketepatan rekomendasi pada dua kasus vignette dispepsia. Penelitian ini menggunakan desain potong lintang dan 42 apoteker berpartisipasi dalam penelitian ini. Instrumen penelitian menggunakan kuesioner yang berisi: (1) karakteristik apoteker dan apotek serta (2) dua kasus vignette: kasus dispepsia tanpa alarm symptoms dan kasus dispepsia karena Na diklofenak. Pengambilan data dilakukan dengan wawancara terstruktur. Analisis data dilakukan dengan inductive content analysis. Ketepatan rekomendasi dinilai berdasarkan literatur dan opini expert panel. Tipe rekomendasi yang paling sering diberikan oleh apoteker pada dua kasus di atas adalah memberikan produk untuk gejala dispepsia. Ketepatan rekomendasi pada kasus dispepsia tanpa alarm symptom dan pada kasus dispepsia karena Na diklofenak secara berturut-turut adalah 83% dan 21% dari 42 apoteker yang berpartisipasi. Dapat disimpulkan bahwa ketepatan rekomendasi bervariasi tergantung kasus. Perlu adanya peningkatan kemampuan apoteker dalam memberikan rekomendasi yang tepat, terutama pada kasus swamedikasi dispepsia yang membutuhkan rujukan ke dokter. \u0000 \u0000 \u0000 \u0000 \u0000 \u0000 \u0000 \u0000 \u0000 \u0000Dyspepsia is a symptom commonly seen in community pharmacies, and therefore pharmacists need to be able to provide appropriate recommendations for patients with dyspepsia. This study aims to describe the types and the appropriateness of the recommendation provided by pharmacists when responding to two vignette cases of dyspepsia. This study was a cross sectional study, and 42 community pharmacists participated in the study. A questionnaire consisting of (1) pharmacists and pharmacy characteristics, and (2) two vignette cases of dyspepsia without alarm symptoms and dyspepsia due to Na diclofenac was developed. A structured interview was used for data collection, and an inductive content analysis was used to analyse the cases. The appropriateness of the recommendation was assessed based on the literature and expert panel opinion. The most common type of recommendation provided in the 2 vignette cases was recommending product for dyspepsia. Appropriate recommendation was provided by 83% and 21% of the 42 participating pharmacists in the case of dyspepsia without alarm symptoms and in the case of dyspepia due to Na diclofenac respectively. The appropriateness of the recommendation varied depending on the case. There is a need to improve the capability of pharmacists in providing appropriate recommendation, particularly for a dyspepsia case that needs medical referral. \u0000 \u0000 \u0000 \u0000","PeriodicalId":18807,"journal":{"name":"MPI (Media Pharmaceutica Indonesiana)","volume":"55 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-12-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"83767898","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Antifungal intervention fails in approximately half of fungal keratitis patients, demonstrating its limitations. Voriconazole use for fungal keratitis has raised new interest because of its broad spectrum and good ocular penetration. However, its effectiveness has not been systematically evaluated. Here we try to clarify the benefits of voriconazole in fungal keratitis cases. Randomized controlled trials (RCTs) comparing voriconazole to placebo or other antifungal medications for fungal keratitis were searched in several databases, including PubMed, Scopus, Cochrane Library, ClinicalTrials, and WHO-ICTRP. The primary outcome that analyzed was best spectacle-corrected vision acuity (BSCVA). The secondary outcomes were treatment success, corneal perforation or need for therapeutic penetrating keratoplasty (TPK). From 621 records, nine studies were selected for analysis. The results were as follows: As an initial therapy, topical natamycin outperformed voriconazole in BSCVA (mean difference = 0.14; 95% CI 0.02 to 0.26; P =.03). Voriconazole also has a greater risk of corneal perforation or TPK than natamycin (RR=1.69; 95% CI 1.11 to 2.58; P=.02). As an adjuvant, there is no significant difference found in BSCVA, treatment success, event of corneal perforation, or need for TPK between voriconazole and the other antifungal agents (itraconazole, ketoconazole, amphotericin B, natamycin, and placebo). This study shows that voriconazole is less superior than natamycin in treating early infections of fungal keratitis. More RCTs with larger samples are needed to evaluate voriconazole's adjuvant efficacy.
抗真菌干预在大约一半的真菌性角膜炎患者中失败，表明其局限性。伏立康唑用于真菌性角膜炎引起了新的兴趣，因为它的广谱和良好的眼部穿透性。然而，其有效性尚未得到系统评价。在这里，我们试图阐明伏立康唑在真菌性角膜炎病例中的益处。在PubMed、Scopus、Cochrane Library、ClinicalTrials和WHO-ICTRP等多个数据库中检索了比较vorigo - nazole与安慰剂或其他抗真菌药物治疗真菌性角膜炎的随机对照试验(rct)。分析的主要结果是最佳眼镜矫正视力(BSCVA)。次要结果是治疗成功，角膜穿孔或需要治疗性穿透性角膜移植术(TPK)。从621项记录中，选择了9项研究进行分析。结果如下:作为初始治疗，局部纳他霉素治疗BSCVA的效果优于vori-conazole(平均差异= 0.14;95% CI 0.02 ~ 0.26;P = 03)。伏立康唑发生角膜穿孔或TPK的风险也高于纳他霉素(RR=1.69;95% CI 1.11 ~ 2.58;P = .02点)。作为辅助治疗，伏立康唑与其他抗真菌药物(伊曲康唑、酮康唑、两性霉素B、那他霉素和安慰剂)在BSCVA、治疗成功率、角膜穿孔事件或TPK需求方面无显著差异。本研究表明伏立康唑在治疗真菌性角膜炎早期感染方面不如那他霉素优越。评估伏立康唑的辅助疗效需要更多的大样本随机对照试验。
{"title":"Effectiveness of Voriconazole in Treating Fungal Keratitis: A Systematic Review and Meta-Analysis of Randomized Controlled Trials","authors":"Yulia Aryati, Elfa Ali Idrus","doi":"10.24123/mpi.v4i2.5284","DOIUrl":"https://doi.org/10.24123/mpi.v4i2.5284","url":null,"abstract":"\u0000 \u0000 \u0000 \u0000Antifungal intervention fails in approximately half of fungal keratitis patients, demonstrating its limitations. Voriconazole use for fungal keratitis has raised new interest because of its broad spectrum and good ocular penetration. However, its effectiveness has not been systematically evaluated. Here we try to clarify the benefits of voriconazole in fungal keratitis cases. Randomized controlled trials (RCTs) comparing voriconazole to placebo or other antifungal medications for fungal keratitis were searched in several databases, including PubMed, Scopus, Cochrane Library, ClinicalTrials, and WHO-ICTRP. The primary outcome that analyzed was best spectacle-corrected vision acuity (BSCVA). The secondary outcomes were treatment success, corneal perforation or need for therapeutic penetrating keratoplasty (TPK). From 621 records, nine studies were selected for analysis. The results were as follows: As an initial therapy, topical natamycin outperformed voriconazole in BSCVA (mean difference = 0.14; 95% CI 0.02 to 0.26; P =.03). Voriconazole also has a greater risk of corneal perforation or TPK than natamycin (RR=1.69; 95% CI 1.11 to 2.58; P=.02). As an adjuvant, there is no significant difference found in BSCVA, treatment success, event of corneal perforation, or need for TPK between voriconazole and the other antifungal agents (itraconazole, ketoconazole, amphotericin B, natamycin, and placebo). This study shows that voriconazole is less superior than natamycin in treating early infections of fungal keratitis. More RCTs with larger samples are needed to evaluate voriconazole's adjuvant efficacy. \u0000 \u0000 \u0000 \u0000","PeriodicalId":18807,"journal":{"name":"MPI (Media Pharmaceutica Indonesiana)","volume":"29 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-12-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"74609041","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Penelitian ini ditujukan untuk menggambarkan implementasi pelayanan telefarmasi pada pelayanan farmasi klinis di apotek yang dipandang dari perspektif apoteker pemberi layanan. Penelitian ini merupakan penelitian deskriptif dengan metode survey menggunakan kuesioner. Kuesioner disusun berdasarkan standar pelayanan kefarmasian di apotek, dan pelayanan farmasi klinis yang terdiri dari 7 domain. Validasi kuesioner terlebih dahulu diuji validitas rupa (face validity). Sampel penelitian terdiri dari 40 apoteker yang berpraktik dan menyediakan pelayanan telefarmasi di apotek jaringan di Surabaya dan Sidoarjo. Sejumlah 34 apoteker yang memenuhi kriteria inklusi dan eksklusi terlibat dalam penelitian ini. Hasil penelitian menunjukkan bahwa pengkajian resep, dispensing dan KIE, pelayanan informasi obat, konseling, pemantauan terapi obat, home pharmacy care, dan monitoring efek samping obat selalu dan sering dilakukan oleh apoteker. Namun, dokumentasi konseling dan pemantauan terapi obat hanya dilakukan oleh 47,1% dan 55,9% responden. Berdasarkan hasil penelitian, dapat disimpulkan bahwa pelayanan telefarmasi pada pelayanan farmasi klinis sudah dilakukan dengan baik oleh apoteker. Telepharmacy services have become part of pharmaceutical services in community pharmacy. Based on regulations, this service must be provided in accordance with pharmaceutical service standards in the community. This study aimed to describe the implementation of telepharmacy services in the community from the perspective of practicing pharmacists. This study is a descriptive research using survey method with a questionnaire. Questionnaires were prepared based on pharmaceutical service standards in pharmacies, and clinical pharmacy services consisting of 7 domains. The validity of the questionnaire was tested for face validity. The sample of this study was consisted of 40 pharmacists who practice and provide telepharmacy services at network pharmacies in Surabaya and Sidoarjo. A total of 34 pharmacists who met the inclusion and exclusion criteria were involved in this study. The results showed that the assessment of prescriptions, dispensing and communication-information-education, drug information services, counseling, monitoring of drug therapy, home pharmacy care, and monitoring of drug side effects were always and often carried out by pharmacists. However, documentation of counseling and monitoring of drug therapy was only carried out by 47.1% and 55.9% of respondents. Based on the results of this study, it can be concluded that telepharmacy in clinical pharmacy services have been carried out by pharmacist properly.
{"title":"Implementasi Pelayanan Telefarmasi di Apotek Sesuai Standar Pelayanan Kefarmasian","authors":"Ivonn Elsa Aulia Puspita, Ika Mulyono Putri Wibowo, Franciscus Cahyo Kristianto","doi":"10.24123/mpi.v4i2.5278","DOIUrl":"https://doi.org/10.24123/mpi.v4i2.5278","url":null,"abstract":"\u0000 \u0000 \u0000 \u0000 \u0000 \u0000 \u0000 \u0000 \u0000 \u0000 \u0000 \u0000Penelitian ini ditujukan untuk menggambarkan implementasi pelayanan telefarmasi pada pelayanan farmasi klinis di apotek yang dipandang dari perspektif apoteker pemberi layanan. Penelitian ini merupakan penelitian deskriptif dengan metode survey menggunakan kuesioner. Kuesioner disusun berdasarkan standar pelayanan kefarmasian di apotek, dan pelayanan farmasi klinis yang terdiri dari 7 domain. Validasi kuesioner terlebih dahulu diuji validitas rupa (face validity). Sampel penelitian terdiri dari 40 apoteker yang berpraktik dan menyediakan pelayanan telefarmasi di apotek jaringan di Surabaya dan Sidoarjo. Sejumlah 34 apoteker yang memenuhi kriteria inklusi dan eksklusi terlibat dalam penelitian ini. Hasil penelitian menunjukkan bahwa pengkajian resep, dispensing dan KIE, pelayanan informasi obat, konseling, pemantauan terapi obat, home pharmacy care, dan monitoring efek samping obat selalu dan sering dilakukan oleh apoteker. Namun, dokumentasi konseling dan pemantauan terapi obat hanya dilakukan oleh 47,1% dan 55,9% responden. Berdasarkan hasil penelitian, dapat disimpulkan bahwa pelayanan telefarmasi pada pelayanan farmasi klinis sudah dilakukan dengan baik oleh apoteker. \u0000 \u0000 \u0000 \u0000 \u0000 \u0000 \u0000 \u0000 \u0000 \u0000 \u0000 \u0000 \u0000 \u0000Telepharmacy services have become part of pharmaceutical services in community pharmacy. Based on regulations, this service must be provided in accordance with pharmaceutical service standards in the community. This study aimed to describe the implementation of telepharmacy services in the community from the perspective of practicing pharmacists. This study is a descriptive research using survey method with a questionnaire. Questionnaires were prepared based on pharmaceutical service standards in pharmacies, and clinical pharmacy services consisting of 7 domains. The validity of the questionnaire was tested for face validity. The sample of this study was consisted of 40 pharmacists who practice and provide telepharmacy services at network pharmacies in Surabaya and Sidoarjo. A total of 34 pharmacists who met the inclusion and exclusion criteria were involved in this study. The results showed that the assessment of prescriptions, dispensing and communication-information-education, drug information services, counseling, monitoring of drug therapy, home pharmacy care, and monitoring of drug side effects were always and often carried out by pharmacists. However, documentation of counseling and monitoring of drug therapy was only carried out by 47.1% and 55.9% of respondents. Based on the results of this study, it can be concluded that telepharmacy in clinical pharmacy services have been carried out by pharmacist properly. \u0000 \u0000 \u0000 \u0000 \u0000 \u0000 \u0000 \u0000","PeriodicalId":18807,"journal":{"name":"MPI (Media Pharmaceutica Indonesiana)","volume":"27 Sup12 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-12-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"90925481","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Alphitonia excelsa merupakan salah satu tanaman yang berpotensi sebagai antioksidan dan telah digunakan secara turun temurun di masyarakat sebagai terapi berbagai macam penyakit. Hal tersebut mendasari tujuan penelitian ini, yaitu menentukan aktivitas antioksidan dan total flavonoid kulit batang A. excelsa. Metode yang di gunakan dalam pengujian antioksidan adalah peredaman terhadap radikal 2,2-difenil-1-pikrilhidrazil (DPPH), sedangkan pengujian flavonoid total dengan AlCl3 dilakukan secara kolorimetri. Hasil yang didapatkan dari penelitian ini adalah nilai IC50 aktivitas antioksidan A. excelsa sebesar 37,00 ± 1,46 μg/ml (bpj) dan flavonoid total senilai 1,17% ± 0.04 QE (quercetin equivalent). Berdasarkan nilai tersebut dapat disimpulkan bahwa kulit batang A. excelsa memiliki kemampuan antioksidan yang sangat kuat. Alphitonia excelsa is a plant that potential to be tested for its antioxidant ability and has been used as medicine. This underlies the purpose of this study, namely to determine the antioxidant activity and total flavonoids of the stem bark of A. excelsa. The method used in antioxidant evaluaion was 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging, while the total flavonoid assay with AlCl3 was carried out colorimetrically. The results obtained from this study were the IC50 value of the antioxidant activity and total flavonoids of A. excelsa, with the value of 37.00 ± 1.46 μg/ml and 1.17% ± 0.04 quercetin equivalent, respectively. Based on these values, it can be concluded that the stem bark of A. excelsa has a very strong antioxidant capacity.
Alphitonia excelsa是一种潜在的抗氧化剂植物，它被广泛用于各种疾病的治疗。这是这项研究的目标，即确定抗氧化物和全黄酮活性A. excelsa皮肤。抗氧化剂测试中使用的方法是缓解正基2，二苯-1- pikril液压(DPPH)，而完全与AlCl3的flavonoid测试是共重度的。螺旋藻的这项研究的结果是价值大小的圆A。抗氧化能力37,00±1,46草根阶层μg / ml()和类黄酮总共价值1,17%±0。04 QE (quercetin哪里)。根据这些值，可以得出结论，茎A. excelsa具有很强的抗氧化剂能力。Alphitonia是一种可能测试其抗氧化性能力并作为药物使用的植物。这项研究的目的不足，需要确定抗氧化剂活性和总正确性的正确性。用于抗氧化剂评估的方法是2,2- 1-picrylhydrazyl (dpplhydrazyl, dpp3)的全黄酮结探(AlCl3)。The results螺旋藻价值》获得来自这个研究是antioxidant活动和援助总计flavonoids of A价值》圆里,用37点±1μg / ml和46。17%±0。04 quercetin哪里,respectively。基于这些价值，它可以得出结论，excelsa的干细胞具有很强的抗氧化剂电容器结构。
{"title":"Uji Flavonoid Total dan Antioksidan Kulit Batang Balik Angin (Alphitonia excelsa (Fenzl) Reis Ex. Endl)","authors":"Samsul Hadi, Ana Muliana, Amalia Khairunnisa","doi":"10.24123/mpi.v4i2.5208","DOIUrl":"https://doi.org/10.24123/mpi.v4i2.5208","url":null,"abstract":"\u0000 \u0000 \u0000 \u0000 \u0000 \u0000 \u0000 \u0000Alphitonia excelsa merupakan salah satu tanaman yang berpotensi sebagai antioksidan dan telah digunakan secara turun temurun di masyarakat sebagai terapi berbagai macam penyakit. Hal tersebut mendasari tujuan penelitian ini, yaitu menentukan aktivitas antioksidan dan total flavonoid kulit batang A. excelsa. Metode yang di gunakan dalam pengujian antioksidan adalah peredaman terhadap radikal 2,2-difenil-1-pikrilhidrazil (DPPH), sedangkan pengujian flavonoid total dengan AlCl3 dilakukan secara kolorimetri. Hasil yang didapatkan dari penelitian ini adalah nilai IC50 aktivitas antioksidan A. excelsa sebesar 37,00 ± 1,46 μg/ml (bpj) dan flavonoid total senilai 1,17% ± 0.04 QE (quercetin equivalent). Berdasarkan nilai tersebut dapat disimpulkan bahwa kulit batang A. excelsa memiliki kemampuan antioksidan yang sangat kuat. \u0000 \u0000 \u0000 \u0000 \u0000 \u0000 \u0000 \u0000 \u0000 \u0000 \u0000 \u0000 \u0000 \u0000Alphitonia excelsa is a plant that potential to be tested for its antioxidant ability and has been used as medicine. This underlies the purpose of this study, namely to determine the antioxidant activity and total flavonoids of the stem bark of A. excelsa. The method used in antioxidant evaluaion was 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging, while the total flavonoid assay with AlCl3 was carried out colorimetrically. The results obtained from this study were the IC50 value of the antioxidant activity and total flavonoids of A. excelsa, with the value of 37.00 ± 1.46 μg/ml and 1.17% ± 0.04 quercetin equivalent, respectively. Based on these values, it can be concluded that the stem bark of A. excelsa has a very strong antioxidant capacity. \u0000 \u0000 \u0000 \u0000 \u0000 \u0000 \u0000 \u0000 \u0000 \u0000 \u0000 \u0000","PeriodicalId":18807,"journal":{"name":"MPI (Media Pharmaceutica Indonesiana)","volume":"11 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-12-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"90675123","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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