Jian Wang, Dehua Liu, Yong Liu, Gongliang Zhang, F. Peng, Zhi Wang
We evaluated the expression of estrogen receptor (ER), progesterone receptor (PR), and C-erbB-2 in patients with ovarian cancer using tissue microarrays (TMA) and preliminarily analyzed molecular typing data. Specimens from 119 ovarian cancer patients were collected and were analyzed by TMA. The expression of ER, PR, and C-erbB-2 was examined by IHC and the clinicopathological associations were analyzed. The results indicated that higher ER expression was observed in SC and EC, whereas PR exhibited a similar expression pattern, but relatively lower compared with ER expression. Conversely, very weak expression was observed in CCC and MC, especially for PR (All P <0.05). C-erbB-2 exhibited no expression pattern differences among the different histological types (All P >0.05), but exhibited higher positive expression in FIGO III and IV stages, whereas there was no difference in ER and PR expression among the different stages. Higher PR expression was observed in middle and highly differentiated tumors, whereas higher C-erbB-2 expression was associated with low degree of differentiation (P <0.05). Patients with ER (+) PR (+) C-erbB-2 (?) had a better prognosis and patients with ER (?) PR (?) C-erbB-2 (+) had the worst prognosis. In conclusion, ER and PR tend to be highly expressed in less malignant ovarian cancer subtypes such as SC and EC. Ovarian cancer patients with ER/PR double-positive and C-erbB-2 negative expression patterns survive longer.
{"title":"Tissue Microarray Detection of Estrogen Receptor, Progesterone Receptor, and C-erbB-2 in Patients with Ovarian Cancer and a Preliminary Study on the Molecular Typing of Ovarian Cancer","authors":"Jian Wang, Dehua Liu, Yong Liu, Gongliang Zhang, F. Peng, Zhi Wang","doi":"10.1166/NNL.2020.3234","DOIUrl":"https://doi.org/10.1166/NNL.2020.3234","url":null,"abstract":"We evaluated the expression of estrogen receptor (ER), progesterone receptor (PR), and C-erbB-2 in patients with ovarian cancer using tissue microarrays (TMA) and preliminarily analyzed molecular typing data. Specimens from 119 ovarian cancer patients were collected and were analyzed\u0000 by TMA. The expression of ER, PR, and C-erbB-2 was examined by IHC and the clinicopathological associations were analyzed. The results indicated that higher ER expression was observed in SC and EC, whereas PR exhibited a similar expression pattern, but relatively lower compared with ER expression.\u0000 Conversely, very weak expression was observed in CCC and MC, especially for PR (All P <0.05). C-erbB-2 exhibited no expression pattern differences among the different histological types (All P >0.05), but exhibited higher positive expression in FIGO III and IV stages, whereas\u0000 there was no difference in ER and PR expression among the different stages. Higher PR expression was observed in middle and highly differentiated tumors, whereas higher C-erbB-2 expression was associated with low degree of differentiation (P <0.05). Patients with ER (+) PR (+) C-erbB-2\u0000 (?) had a better prognosis and patients with ER (?) PR (?) C-erbB-2 (+) had the worst prognosis. In conclusion, ER and PR tend to be highly expressed in less malignant ovarian cancer subtypes such as SC and EC. Ovarian cancer patients with ER/PR double-positive and C-erbB-2 negative expression\u0000 patterns survive longer.","PeriodicalId":18871,"journal":{"name":"Nanoscience and Nanotechnology Letters","volume":"12 1","pages":"1159-1165"},"PeriodicalIF":0.0,"publicationDate":"2020-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"48784901","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
C. Pan, Guoqiu Xu, Bin Xu, Wei Gan, Yunkun Liu, Guangxia Wei, Lei Jiang, Yunhe Huang, C. Ye
In thoracoscopic segmentectomy, accurate preoperative identification of intersegmental vessels, bronchi, and the surgical safety margin is vital. We applied three dimensional computed tomography bronchography and angiography (3D-CTBA) reconstruction to appropriately plan thoracoscopic segmentectomy for Patients with pulmonary nodules. In this study, we evaluated the effectiveness and accuracy of 3D-CTBA reconstruction for the identification of segmental anatomical structures and variation during thoracoscopic segmentectomy.We retrospectively analyzed data of 30 patients who underwent 3D-CTBA reconstruction before thoracoscopic segmentectomy between January and May 2019 in the Department of Thoracic Surgery, First Affiliated Hospital of Nanchang University. We compared the individual target segment arteries, veins, and bronchi identified during surgery with the preoperative 3D-CTBA model to evaluate its effectiveness and accuracy. The accuracy of the preoperative 3D-CTBA model for the identification of target segmental arteries, veins, and bronchi was 99.08% (108/109), 98.39% (122/124), and 100% (118/118), respectively. Through 3DCTBA modeling, we found mediastinal and interlobar types of lingular segmental arteries in six patients, and central veins were not found in seven patients. In addition, we detected rare anatomical variations in two patients; one patient had the right apical segmental bronchus that stemmed solely from the right primary bronchus (tracheal bronchus), and the other had rare right basal segmental variant bronchi and vessels. The 3D-CTBA model can precisely predict segmental bronchi and vessels and identify anatomical structure variations before operation, which can aid surgeons to avoid incorrect operation and improve surgical efficiency. This has important implications for thoracoscopic segmentectomy.
{"title":"Application of Three-Dimensional Computed Tomography Bronchography and Angiography Reconstruction in Thoracoscopic Segmentectomy and Segmental Structure Analysis","authors":"C. Pan, Guoqiu Xu, Bin Xu, Wei Gan, Yunkun Liu, Guangxia Wei, Lei Jiang, Yunhe Huang, C. Ye","doi":"10.1166/NNL.2020.3218","DOIUrl":"https://doi.org/10.1166/NNL.2020.3218","url":null,"abstract":"In thoracoscopic segmentectomy, accurate preoperative identification of intersegmental vessels, bronchi, and the surgical safety margin is vital. We applied three dimensional computed tomography bronchography and angiography (3D-CTBA) reconstruction to appropriately plan thoracoscopic\u0000 segmentectomy for Patients with pulmonary nodules. In this study, we evaluated the effectiveness and accuracy of 3D-CTBA reconstruction for the identification of segmental anatomical structures and variation during thoracoscopic segmentectomy.We retrospectively analyzed data of 30 patients\u0000 who underwent 3D-CTBA reconstruction before thoracoscopic segmentectomy between January and May 2019 in the Department of Thoracic Surgery, First Affiliated Hospital of Nanchang University. We compared the individual target segment arteries, veins, and bronchi identified during surgery with\u0000 the preoperative 3D-CTBA model to evaluate its effectiveness and accuracy. The accuracy of the preoperative 3D-CTBA model for the identification of target segmental arteries, veins, and bronchi was 99.08% (108/109), 98.39% (122/124), and 100% (118/118), respectively. Through 3DCTBA modeling,\u0000 we found mediastinal and interlobar types of lingular segmental arteries in six patients, and central veins were not found in seven patients. In addition, we detected rare anatomical variations in two patients; one patient had the right apical segmental bronchus that stemmed solely from the\u0000 right primary bronchus (tracheal bronchus), and the other had rare right basal segmental variant bronchi and vessels. The 3D-CTBA model can precisely predict segmental bronchi and vessels and identify anatomical structure variations before operation, which can aid surgeons to avoid incorrect\u0000 operation and improve surgical efficiency. This has important implications for thoracoscopic segmentectomy.","PeriodicalId":18871,"journal":{"name":"Nanoscience and Nanotechnology Letters","volume":"12 1","pages":"1137-1143"},"PeriodicalIF":0.0,"publicationDate":"2020-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"44336574","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
X. Zhou, Chuan‐Yu Zhang, Song Pu, Kai Yang, Zhe Shi
A physical model of melting furnace for producing molten iron is established. Paraffin particles are used to simulate ores. Corn particles are used to simulate coke. The gas phase temperature in the furnace was measured with a temperature sensor. The temperature gradient analysis was used to study the effect of cohesive zone on the material layer. The study found that the higher the melting point of the experimental materials, the higher the cohesive zone temperature, resulting in the higher the overall temperature of the material layer, which is conducive to improving the utilization rate of gas. At the same time, the heat transfer process of cohesive zone will become more intense. The results presented in this paper will have certain guiding significance for the research of cohesive zone and solid layer of blast furnace.
{"title":"Physical Simulation Study on Influence of Iron Ore Melting Point on Cohesive Zone and Material Layer","authors":"X. Zhou, Chuan‐Yu Zhang, Song Pu, Kai Yang, Zhe Shi","doi":"10.1166/NNL.2020.3220","DOIUrl":"https://doi.org/10.1166/NNL.2020.3220","url":null,"abstract":"A physical model of melting furnace for producing molten iron is established. Paraffin particles are used to simulate ores. Corn particles are used to simulate coke. The gas phase temperature in the furnace was measured with a temperature sensor. The temperature gradient analysis was\u0000 used to study the effect of cohesive zone on the material layer. The study found that the higher the melting point of the experimental materials, the higher the cohesive zone temperature, resulting in the higher the overall temperature of the material layer, which is conducive to improving\u0000 the utilization rate of gas. At the same time, the heat transfer process of cohesive zone will become more intense. The results presented in this paper will have certain guiding significance for the research of cohesive zone and solid layer of blast furnace.","PeriodicalId":18871,"journal":{"name":"Nanoscience and Nanotechnology Letters","volume":"12 1","pages":"1095-1098"},"PeriodicalIF":0.0,"publicationDate":"2020-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"46387986","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Shengchi Zhang, Li Li, Xianfeng Wei, X. Mao, Xueqing Zhang, Yao Liu, M. Wei, Wei Wang, Luna Zhang
Tumor hypoxia is considered as an important factor for tumor metastasis and disease recurrence. Evofosfamide (TH-302) is a hypoxic prodrug, which can selectively target the hypoxic area of solid tumors, and has the potential to improve the efficacy of the commercial anticancer drug afatinib (AFT). However, free hydrophobic AFT and hydrophilic TH-302 still have several unequivocal deficiencies, such as unsatisfactory tumor inhibition rate, serious side effects and being easy to induce multidrug resistance. Moreover, the operation process of co-administration is too complicated. Therefore, this paper discussed the synergistic effects of AFT/TH-302 and developed a kind of co-loaded targeted mesoporous silica nanoparticles (MSNs) for the treatment of nasopharyngeal carcinoma (NPC). The calculated proportion of AFT and TH-302 were encapsulated by folic acid (FA) modified MSNs (FA-MSNs). In vitro experiments showed that free AFT and TH-302 had synergistic effect, while MSNs nanocarrier could significantly reduce the half maximal inhibitory concentrations (IC50) of AFT and TH-302. AFT and TH-302 show significant synergistic on NPC cells, the application of MSN carrier platform including fixed proportion of AFT and TH-302 improves the synergistic effect and provides a new idea for the treatment of NPC.
{"title":"Targeting Mesoporous Silica for Synergistic TH-302 and Afatinib Delivery to Nasopharyngeal Carcinoma","authors":"Shengchi Zhang, Li Li, Xianfeng Wei, X. Mao, Xueqing Zhang, Yao Liu, M. Wei, Wei Wang, Luna Zhang","doi":"10.1166/NNL.2020.3211","DOIUrl":"https://doi.org/10.1166/NNL.2020.3211","url":null,"abstract":"Tumor hypoxia is considered as an important factor for tumor metastasis and disease recurrence. Evofosfamide (TH-302) is a hypoxic prodrug, which can selectively target the hypoxic area of solid tumors, and has the potential to improve the efficacy of the commercial anticancer drug\u0000 afatinib (AFT). However, free hydrophobic AFT and hydrophilic TH-302 still have several unequivocal deficiencies, such as unsatisfactory tumor inhibition rate, serious side effects and being easy to induce multidrug resistance. Moreover, the operation process of co-administration is too complicated.\u0000 Therefore, this paper discussed the synergistic effects of AFT/TH-302 and developed a kind of co-loaded targeted mesoporous silica nanoparticles (MSNs) for the treatment of nasopharyngeal carcinoma (NPC). The calculated proportion of AFT and TH-302 were encapsulated by folic acid (FA) modified\u0000 MSNs (FA-MSNs). In vitro experiments showed that free AFT and TH-302 had synergistic effect, while MSNs nanocarrier could significantly reduce the half maximal inhibitory concentrations (IC50) of AFT and TH-302. AFT and TH-302 show significant synergistic on NPC cells, the application of MSN\u0000 carrier platform including fixed proportion of AFT and TH-302 improves the synergistic effect and provides a new idea for the treatment of NPC.","PeriodicalId":18871,"journal":{"name":"Nanoscience and Nanotechnology Letters","volume":"12 1","pages":"1127-1136"},"PeriodicalIF":0.0,"publicationDate":"2020-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"45526012","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Because of its antibacterial properties, wormwood can be used in the production of nanobiomaterials. In this paper, each stage of the production process of wormwood viscose fiber and flax fiber was determined. The carbon emission of each stage of the production process of 1 ton wormwood viscose fiber and flax fiber was analyzed by GaBi software, and the environmental impact of the production process was evaluated by using the CML2001 method provided by the software. The results showed that a total of 1690.04 kg of carbon dioxide was emitted in the production of 1 ton of wormwood viscose fiber, 60% in the preparation stage, 36.36% in the acid bath stage and 3.64% in the treatment stage. A total of 1541.41 kg of carbon dioxide was emitted in the production of 1 ton of flax fiber, with the pretreatment stage accounting for 39.95% of the total amount, the alkali cooking stage accounting for 50.06% of the total amount, and the pickling stage accounting for 9.99% of the total amount. The results can provide support for the production of antibacterial nanofibers.
{"title":"A Comparative Study of Carbon Emission of Wormwood Viscose Fiber and Flax Fiber for the Production of Antibacterial Nanofibers Based on GaBi Software","authors":"Jin Zhang, X. Qian, Jing Feng, Hui Liu","doi":"10.1166/NNL.2020.3213","DOIUrl":"https://doi.org/10.1166/NNL.2020.3213","url":null,"abstract":"Because of its antibacterial properties, wormwood can be used in the production of nanobiomaterials. In this paper, each stage of the production process of wormwood viscose fiber and flax fiber was determined. The carbon emission of each stage of the production process of 1 ton wormwood\u0000 viscose fiber and flax fiber was analyzed by GaBi software, and the environmental impact of the production process was evaluated by using the CML2001 method provided by the software. The results showed that a total of 1690.04 kg of carbon dioxide was emitted in the production of 1 ton of wormwood\u0000 viscose fiber, 60% in the preparation stage, 36.36% in the acid bath stage and 3.64% in the treatment stage. A total of 1541.41 kg of carbon dioxide was emitted in the production of 1 ton of flax fiber, with the pretreatment stage accounting for 39.95% of the total amount, the alkali cooking\u0000 stage accounting for 50.06% of the total amount, and the pickling stage accounting for 9.99% of the total amount. The results can provide support for the production of antibacterial nanofibers.","PeriodicalId":18871,"journal":{"name":"Nanoscience and Nanotechnology Letters","volume":"12 1","pages":"1144-1149"},"PeriodicalIF":0.0,"publicationDate":"2020-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"48861657","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
W. Haifeng, Chen Xiaoliang, Zhao Ping-yuan, Gao Zhaowei, You Xiaoyu, Tian Jiayu, Wang Jiawei
Manganese sulfate solution was in this study oxidized, using H2O2 as oxidant, to obtain new Nano-MnO2 in situ. The characterization of new MnO2 was carried out by XRD, SEM, FT-IR and BET. The effect of new manganese dioxide adsorpting molybdenum ion in manganese sulfate solution was also studied. Results showed that the main crystal form of the new MnO2 was γ type, and there was agglomeration of nanospheres with 200∼300 nm diameter from the microscopic morphology, which had abundant surface hydroxyl groups, and its specific surface area was as high as 146 m2/g. MnO2 addition was 1.0 g under optimal adsorption conditions when the pH was 2, and the reaction time was 30 min, with removal rate of 99.2% molybdenum and 0.26 ppm residual amount of molybdenum in manganese sulfate solution, which met the quality standard for high purity manganese sulfate (HG/T4823-2015) with ≤5 ppm molybdenum content. Moreover, the thermodynamics and kinetics of molybdenum adsorption by new manganese dioxide were also studied. The experiments showed that the adsorption process was in accordance with the Freundlich adsorption equation. The adsorption process of molybdenum on manganese dioxide could be described with pseudo second order kinetic model, and the internal diffusion was a controlling link of adsorption rate.
{"title":"Preparation of New Nano-MnO2 and Its Molybdenum Adsorption in Manganese Sulfate Solution","authors":"W. Haifeng, Chen Xiaoliang, Zhao Ping-yuan, Gao Zhaowei, You Xiaoyu, Tian Jiayu, Wang Jiawei","doi":"10.1166/NNL.2020.3210","DOIUrl":"https://doi.org/10.1166/NNL.2020.3210","url":null,"abstract":"Manganese sulfate solution was in this study oxidized, using H2O2 as oxidant, to obtain new Nano-MnO2 in situ. The characterization of new MnO2 was carried out by XRD, SEM, FT-IR and BET. The effect of new manganese dioxide adsorpting\u0000 molybdenum ion in manganese sulfate solution was also studied. Results showed that the main crystal form of the new MnO2 was γ type, and there was agglomeration of nanospheres with 200∼300 nm diameter from the microscopic morphology, which had abundant surface\u0000 hydroxyl groups, and its specific surface area was as high as 146 m2/g. MnO2 addition was 1.0 g under optimal adsorption conditions when the pH was 2, and the reaction time was 30 min, with removal rate of 99.2% molybdenum and 0.26 ppm residual amount of molybdenum in\u0000 manganese sulfate solution, which met the quality standard for high purity manganese sulfate (HG/T4823-2015) with ≤5 ppm molybdenum content. Moreover, the thermodynamics and kinetics of molybdenum adsorption by new manganese dioxide were also studied. The experiments showed that the adsorption\u0000 process was in accordance with the Freundlich adsorption equation. The adsorption process of molybdenum on manganese dioxide could be described with pseudo second order kinetic model, and the internal diffusion was a controlling link of adsorption rate.","PeriodicalId":18871,"journal":{"name":"Nanoscience and Nanotechnology Letters","volume":"12 1","pages":"1070-1078"},"PeriodicalIF":0.0,"publicationDate":"2020-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"48173215","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Ovarian cancer (OC) is a common form of gynecological cancer that increases in incidence annually, seriously threatening the health and safety of patients. The critical role of miR-575 in multiple neoplastic diseases has already been well established. We suspected that miRNA-575 may be strongly associated with the occurrence of OC and may be the key to OC diagnosis and treatment in the future. To test this hypothesis, we selected 68 OC patients (research group, or RG) who were admitted to our hospital and 60 concurrent healthy controls (control group, or CG) as the study participants. Human OC cell lines A2780, OVCAR-3, and SK-OV-3, and human normal ovarian epithelial cells IOSE80 were used in this study. Nanoparticle-assisted polymerase chain reaction (Nano-PCR), was used to identify miRNA-575 expression and its diagnostic implications in OC. miRNA-575 expression in OC cells and its influence on OC cell biological behaviors were investigated, and the correlation between miR-575 and the phosphatase and tensin homolog (PTEN) was discussed. The results revealed a highly expressed miRNA-575 in OC. Detection of biological behaviors was conducted after transfection of miRNA-575 into OC cells. Cell proliferation and invasion in the mimics-miRNA-575 group was statistically enhanced compared to the negative control (NC) and ininhibition-miRNA-575 groups, and the apoptosis and apoptosis protein count significantly declined (P < 0.05). Subsequently, PTEN was observed to present a lower expression in OC cells. Online target gene analysis website (http://www.targetscan.org/vert_72/) identified the related sites that could bind miR-575 and PTEN. PTEN protein detection in the mimics-miR-575, ininhibition-miR-575, and NC groups showed that the PTEN protein expression in the mimics-miR-575 group was reduced in comparison with that of the other groups, while that for ininhibition-miR-575 was elevated compared to that of the NC group (P < 0.05). Therefore, we came to the conclusion that a high expression of miR-575 can bolster OC cell proliferation and invasion and inhibit apoptosis, while the impact of miR-575 on OC cells may be achieved via targeting the PTEN protein. miR-575 presents important clinical applications in OC diagnosis as an underlying therapeutic target of OC and is a major breakthrough toward future OC research.
{"title":"Clinical Significance of miR-575 in Ovarian Cancer and Its Effect on Ovarian Cancer Cells","authors":"Jie Wang, Wei-xin Zhou, Xilun Gan, Jianbo Yang","doi":"10.1166/NNL.2020.3221","DOIUrl":"https://doi.org/10.1166/NNL.2020.3221","url":null,"abstract":"Ovarian cancer (OC) is a common form of gynecological cancer that increases in incidence annually, seriously threatening the health and safety of patients. The critical role of miR-575 in multiple neoplastic diseases has already been well established. We suspected that miRNA-575 may\u0000 be strongly associated with the occurrence of OC and may be the key to OC diagnosis and treatment in the future. To test this hypothesis, we selected 68 OC patients (research group, or RG) who were admitted to our hospital and 60 concurrent healthy controls (control group, or CG) as the study\u0000 participants. Human OC cell lines A2780, OVCAR-3, and SK-OV-3, and human normal ovarian epithelial cells IOSE80 were used in this study. Nanoparticle-assisted polymerase chain reaction (Nano-PCR), was used to identify miRNA-575 expression and its diagnostic implications in OC. miRNA-575 expression\u0000 in OC cells and its influence on OC cell biological behaviors were investigated, and the correlation between miR-575 and the phosphatase and tensin homolog (PTEN) was discussed. The results revealed a highly expressed miRNA-575 in OC. Detection of biological behaviors was conducted after transfection\u0000 of miRNA-575 into OC cells. Cell proliferation and invasion in the mimics-miRNA-575 group was statistically enhanced compared to the negative control (NC) and ininhibition-miRNA-575 groups, and the apoptosis and apoptosis protein count significantly declined (P < 0.05). Subsequently,\u0000 PTEN was observed to present a lower expression in OC cells. Online target gene analysis website (http://www.targetscan.org/vert_72/) identified the related sites that could bind miR-575 and PTEN. PTEN protein detection in the mimics-miR-575, ininhibition-miR-575, and NC groups showed that\u0000 the PTEN protein expression in the mimics-miR-575 group was reduced in comparison with that of the other groups, while that for ininhibition-miR-575 was elevated compared to that of the NC group (P < 0.05). Therefore, we came to the conclusion that a high expression of miR-575 can\u0000 bolster OC cell proliferation and invasion and inhibit apoptosis, while the impact of miR-575 on OC cells may be achieved via targeting the PTEN protein. miR-575 presents important clinical applications in OC diagnosis as an underlying therapeutic target of OC and is a major breakthrough toward\u0000 future OC research.","PeriodicalId":18871,"journal":{"name":"Nanoscience and Nanotechnology Letters","volume":"12 1","pages":"1087-1094"},"PeriodicalIF":0.0,"publicationDate":"2020-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"42054143","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
This work evaluated the quality and disinfection efficacy of nano-emulsified cresol disinfectant on common microorganisms in biosafety cabinets (BSCs). Different spraying methods (standard single spray and pulse spray) were used to investigate the effects of different doses and sealing time on the disinfection efficacy of two kinds of nano-emulsified cresol disinfectants. The results show that the nano-emulsified cresol disinfectant can kill bacteria completely when the spraying amount is more than 30 mL and the sealing time is more than 60 min. Nano-emulsified cresol disinfectant can be used for environmental disinfection in the working area of BSCs, and it is an ideal method to replace formaldehyde and chlorine dioxide in the disinfection of BSCs.
{"title":"The Efficacy of Nano-Emulsified Disinfectant in Disinfecting the Workplace Environment","authors":"Na-Kyoung You, Shouyan Wang, Huiju Han","doi":"10.1166/NNL.2020.3216","DOIUrl":"https://doi.org/10.1166/NNL.2020.3216","url":null,"abstract":"This work evaluated the quality and disinfection efficacy of nano-emulsified cresol disinfectant on common microorganisms in biosafety cabinets (BSCs). Different spraying methods (standard single spray and pulse spray) were used to investigate the effects of different doses and sealing\u0000 time on the disinfection efficacy of two kinds of nano-emulsified cresol disinfectants. The results show that the nano-emulsified cresol disinfectant can kill bacteria completely when the spraying amount is more than 30 mL and the sealing time is more than 60 min. Nano-emulsified cresol disinfectant\u0000 can be used for environmental disinfection in the working area of BSCs, and it is an ideal method to replace formaldehyde and chlorine dioxide in the disinfection of BSCs.","PeriodicalId":18871,"journal":{"name":"Nanoscience and Nanotechnology Letters","volume":"12 1","pages":"1114-1119"},"PeriodicalIF":0.0,"publicationDate":"2020-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"44274682","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Yunyan Li, Yuan-Ting Yang, Jian-Feng Chen, Yuxue Wang, Yong Zhang, Yan Zhou, Z. Wang, Yongping Lu
Venous thromboembolism (VTE) is the comorbidity of deep vein thrombosis (DVT) and pulmonary embolism (PE); it is an urgent public health problem. The primary cause of venous thrombosis is endothelial dysfunction caused by vascular injury and inflammation or overexpressed procoagulant factors. Previous studies have shown that vascular endothelial cell apoptosis is involved in venous thrombosis, causing vascular wall damage. The pro-inflammatory cytokine interleukin-17 (Il-17A) induces endothelial cell apoptosis and promotes thrombosis. However, it remains unclear whether other IL-17 family cytokines are involved in thrombus formation. Among the IL-17 family, IL-17B is less well-characterized. Several studies have reported that IL-17B could stimulate TNF-α, IL-1, and IL-6 expression in macrophages. Furthermore, IL-17B induced activation of the ERK1/2 pathway, upregulating Bcl-2 family anti-apoptotic proteins in breast tumors. However, it is unclear whether IL-17B is involved in thrombus formation by regulating endothelial apoptosis. Therefore, this study aimed to examine whether IL-17B could affect endothelial apoptosis by promoting thrombus formation.
{"title":"IL-17B Regulates Endothelial Cell Apoptosis in Venous Thrombosis","authors":"Yunyan Li, Yuan-Ting Yang, Jian-Feng Chen, Yuxue Wang, Yong Zhang, Yan Zhou, Z. Wang, Yongping Lu","doi":"10.1166/NNL.2020.3212","DOIUrl":"https://doi.org/10.1166/NNL.2020.3212","url":null,"abstract":"Venous thromboembolism (VTE) is the comorbidity of deep vein thrombosis (DVT) and pulmonary embolism (PE); it is an urgent public health problem. The primary cause of venous thrombosis is endothelial dysfunction caused by vascular injury and inflammation or overexpressed procoagulant\u0000 factors. Previous studies have shown that vascular endothelial cell apoptosis is involved in venous thrombosis, causing vascular wall damage. The pro-inflammatory cytokine interleukin-17 (Il-17A) induces endothelial cell apoptosis and promotes thrombosis. However, it remains unclear whether\u0000 other IL-17 family cytokines are involved in thrombus formation. Among the IL-17 family, IL-17B is less well-characterized. Several studies have reported that IL-17B could stimulate TNF-α, IL-1, and IL-6 expression in macrophages. Furthermore, IL-17B induced activation of the\u0000 ERK1/2 pathway, upregulating Bcl-2 family anti-apoptotic proteins in breast tumors. However, it is unclear whether IL-17B is involved in thrombus formation by regulating endothelial apoptosis. Therefore, this study aimed to examine whether IL-17B could affect endothelial apoptosis by promoting\u0000 thrombus formation.","PeriodicalId":18871,"journal":{"name":"Nanoscience and Nanotechnology Letters","volume":"12 1","pages":"1106-1113"},"PeriodicalIF":0.0,"publicationDate":"2020-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"44660179","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Li Wei, Lijun Liu, Lin Chen, Xiaoning Li, Zaiyan Wang, Qiubo Wang, Hao Chen, Li Han, Xiao-Bin Ji, Y. Miao, Zeming Zhang
This study investigated whether long noncoding RNA interfering EIF3J antisense RNA1 (EIF3JAS1) could affect lung cancer A549 cells as well as the role of microRNA-330-5p (miR-330-5p) during this process. To this end, quantitative real-time polymerase chain reaction was used to measure EIF3J-AS1 and miR-330-5p expression in 39 lung cancer cases. Small interfering RNA targeting EIF3J-AS1 (si-EIF3J-AS1), as well as the miR-330-5p inhibitor, was transfected into lung cancer A549 cells. The outcomes of cell proliferation, clone formation, migration, invasion, and E- and N-cadherin expression were analyzed using CCK-8 kit, clone formation experiment, Transwell method, and Western blot. The targeted binding between EIF3J-AS1 and miR-330-5p was explored using the luciferase experiment. The results showed higher EIF3J-AS1 expression but lower miR-330-5p expression cancer tissues. Furthermore, interfering EIF3J-AS1 increased miR-330-5p and E-cadherin protein expression, leading to a reduction in the proliferation, clone formation, migration, invasion, and N-cadherin protein expression of lung cancer A549 cells. Meanwhile, Transfecting si-EIF3J-AS1 and the miR-330-5p inhibitor could increase the proliferation, clone formation, migration, invasion, and N-cadherin protein expression of lung cancer A549 cells, suggesting the targeted relationship of EIF3J-AS1 to miR-330-5p. In summary, EIF3J-AS1 was highly expressed in lung cancer tissues, and interfering EIF3J-AS1 inhibited the proliferation,migration, and invasion of A549 cells through negative regulation of miR-330-5p.
{"title":"Interfering Eukaryotic Translation Initiation Factor 3 Subunit J Antisense RNA1 Inhibits the Proliferation, Migration, and Invasion of Lung Cancer A549 Cells by Regulating microRNA-330-5p","authors":"Li Wei, Lijun Liu, Lin Chen, Xiaoning Li, Zaiyan Wang, Qiubo Wang, Hao Chen, Li Han, Xiao-Bin Ji, Y. Miao, Zeming Zhang","doi":"10.1166/NNL.2020.3208","DOIUrl":"https://doi.org/10.1166/NNL.2020.3208","url":null,"abstract":"This study investigated whether long noncoding RNA interfering EIF3J antisense RNA1 (EIF3JAS1) could affect lung cancer A549 cells as well as the role of microRNA-330-5p (miR-330-5p) during this process. To this end, quantitative real-time polymerase chain reaction was used to measure\u0000 EIF3J-AS1 and miR-330-5p expression in 39 lung cancer cases. Small interfering RNA targeting EIF3J-AS1 (si-EIF3J-AS1), as well as the miR-330-5p inhibitor, was transfected into lung cancer A549 cells. The outcomes of cell proliferation, clone formation, migration, invasion, and E- and N-cadherin\u0000 expression were analyzed using CCK-8 kit, clone formation experiment, Transwell method, and Western blot. The targeted binding between EIF3J-AS1 and miR-330-5p was explored using the luciferase experiment. The results showed higher EIF3J-AS1 expression but lower miR-330-5p expression cancer\u0000 tissues. Furthermore, interfering EIF3J-AS1 increased miR-330-5p and E-cadherin protein expression, leading to a reduction in the proliferation, clone formation, migration, invasion, and N-cadherin protein expression of lung cancer A549 cells. Meanwhile, Transfecting si-EIF3J-AS1 and the miR-330-5p\u0000 inhibitor could increase the proliferation, clone formation, migration, invasion, and N-cadherin protein expression of lung cancer A549 cells, suggesting the targeted relationship of EIF3J-AS1 to miR-330-5p. In summary, EIF3J-AS1 was highly expressed in lung cancer tissues, and interfering\u0000 EIF3J-AS1 inhibited the proliferation,migration, and invasion of A549 cells through negative regulation of miR-330-5p.","PeriodicalId":18871,"journal":{"name":"Nanoscience and Nanotechnology Letters","volume":"12 1","pages":"1099-1105"},"PeriodicalIF":0.0,"publicationDate":"2020-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41797772","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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