Dehai Zhang, Junheng Li, Huimin Zhu, Shujun Liu, K. Yu, Z. Guo, Liang Fu
National handcrafts are well-known at home and Abroad for their fine ingredients, exquisite blending of colors, and excellent skilled shaping and painting. What is more, they have great value and are deeply popular with people around the world. National handcrafts, as a cultural crystallization, must be protected. In order to better realize the protection, we try to apply the technology of reverse engineering to the protection. This article mainly takes a handcraft, Yan Zi YU (meaning “swallow fish”), as an example to expound and demonstrate the process of reverse engineering technology. During this process, by using scanning technology of reverse engineering, the collection and splicing of the point cloud data, the theoretical analysis of the NURBS surface in the reconstruction phase, and other practical operations in other stages, reverse engineering can be used to regenerate 3D modal data of the swallow fish and to inspect and analyse the errors in it through Geomagic Quality to ensure that the new model meets the precision requirements. Therefore, the reverse engineering technology can be completely applied to the protection of national handcrafts so that they can fully demonstrate their own value and function. If the precision of reverse engineering detection can reach nanometric level, a breakthrough will promote the protection of cultural relics.
{"title":"The Researches and Applications of Reverse Engineering in the Protection and Inheritance of National Handcrafts","authors":"Dehai Zhang, Junheng Li, Huimin Zhu, Shujun Liu, K. Yu, Z. Guo, Liang Fu","doi":"10.1166/NNL.2020.3215","DOIUrl":"https://doi.org/10.1166/NNL.2020.3215","url":null,"abstract":"National handcrafts are well-known at home and Abroad for their fine ingredients, exquisite blending of colors, and excellent skilled shaping and painting. What is more, they have great value and are deeply popular with people around the world. National handcrafts, as a cultural crystallization,\u0000 must be protected. In order to better realize the protection, we try to apply the technology of reverse engineering to the protection. This article mainly takes a handcraft, Yan Zi YU (meaning “swallow fish”), as an example to expound and demonstrate the process of reverse engineering\u0000 technology. During this process, by using scanning technology of reverse engineering, the collection and splicing of the point cloud data, the theoretical analysis of the NURBS surface in the reconstruction phase, and other practical operations in other stages, reverse engineering can be used\u0000 to regenerate 3D modal data of the swallow fish and to inspect and analyse the errors in it through Geomagic Quality to ensure that the new model meets the precision requirements. Therefore, the reverse engineering technology can be completely applied to the protection of national handcrafts\u0000 so that they can fully demonstrate their own value and function. If the precision of reverse engineering detection can reach nanometric level, a breakthrough will promote the protection of cultural relics.","PeriodicalId":18871,"journal":{"name":"Nanoscience and Nanotechnology Letters","volume":"12 1","pages":"1063-1069"},"PeriodicalIF":0.0,"publicationDate":"2020-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"48258605","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
At present, chemical biosensors still have poor reproducibility and stability due to that the density of nanomaterials particles is not ideal. Therefore, an optimal design method of electrochemical biosensor based on gold nanomaterials is proposed. Two types of sensors, namely DNA biosensor and immune biosensor, were designed in this work. A DNA biosensor with gold nanorod nanocomposite modified electrode was prepared, and its electrical activity was measured by Methylene blue. In contrast, an electrochemical immune sensor with gold nanorod-modified electrode was prepared and used for detection of alpha fetoprotein. The experimental results show that the designed DNA biosensor and electrochemical immune sensor have the advantages of simple preparation, high sensitivity, good selectivity and good stability.
{"title":"Optimization Design of Electrochemical Biosensors Based on Gold Nanomaterials","authors":"Haitao Hao","doi":"10.1166/NNL.2020.3219","DOIUrl":"https://doi.org/10.1166/NNL.2020.3219","url":null,"abstract":"At present, chemical biosensors still have poor reproducibility and stability due to that the density of nanomaterials particles is not ideal. Therefore, an optimal design method of electrochemical biosensor based on gold nanomaterials is proposed. Two types of sensors, namely DNA biosensor\u0000 and immune biosensor, were designed in this work. A DNA biosensor with gold nanorod nanocomposite modified electrode was prepared, and its electrical activity was measured by Methylene blue. In contrast, an electrochemical immune sensor with gold nanorod-modified electrode was prepared and\u0000 used for detection of alpha fetoprotein. The experimental results show that the designed DNA biosensor and electrochemical immune sensor have the advantages of simple preparation, high sensitivity, good selectivity and good stability.","PeriodicalId":18871,"journal":{"name":"Nanoscience and Nanotechnology Letters","volume":"12 1","pages":"1079-1086"},"PeriodicalIF":0.0,"publicationDate":"2020-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"46974233","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Hand soft tissue defects are a common disease in surgical emergency, which seriously affects the limb function of patients and significantly reduces their quality of life. To effectively repair hand soft tissue defects, a difficult problem posed to surgeons, we report here the use of a chitosan-based nanocomposite hydrogel (CS-GEL-PDRN). In vitro experiments showed that the nanocomposite hydrogel could promote the proliferation of human embryonic fibroblasts M-22 and human vascular endothelial cells EC-304. In addition, the hydrogel had excellent blood compatibility. In the rabbit model of soft tissue defects, CS-GEL-PDRN effectively coordinated cytokine responses, accelerated wound healing and promoted tissue repair. Based on this, CS-GEL-PDRN is expected to play a clinical role in the repair of hand and other soft tissue defects in the future.
{"title":"Study on Chitosan-Based Nanocomposite Hydrogel in Soft Tissue Defect of Hand","authors":"Jian Zhang, Zhao-xia Gao, Yao Zhang, J. Tian","doi":"10.1166/NNL.2020.3217","DOIUrl":"https://doi.org/10.1166/NNL.2020.3217","url":null,"abstract":"Hand soft tissue defects are a common disease in surgical emergency, which seriously affects the limb function of patients and significantly reduces their quality of life. To effectively repair hand soft tissue defects, a difficult problem posed to surgeons, we report here the use of\u0000 a chitosan-based nanocomposite hydrogel (CS-GEL-PDRN). In vitro experiments showed that the nanocomposite hydrogel could promote the proliferation of human embryonic fibroblasts M-22 and human vascular endothelial cells EC-304. In addition, the hydrogel had excellent blood compatibility. In\u0000 the rabbit model of soft tissue defects, CS-GEL-PDRN effectively coordinated cytokine responses, accelerated wound healing and promoted tissue repair. Based on this, CS-GEL-PDRN is expected to play a clinical role in the repair of hand and other soft tissue defects in the future.","PeriodicalId":18871,"journal":{"name":"Nanoscience and Nanotechnology Letters","volume":"69 20","pages":"1120-1126"},"PeriodicalIF":0.0,"publicationDate":"2020-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41249655","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Colorectal adenocarcinoma (COAD) is the most common type of colon cancer, and its occurrence is intimately associated with immune-related gene (IRG) expression. In this research, the IRG expression was analyzed and 484 were found significantly different between COAD and normal tissue. To construct a COAD patient IRGs-prognosis model, 11 of the IRGs were selected by multivariate Cox analysis. IRGs in the model were closely related to tumor invasion depth, lymphoid involvement, distant metastasis, stage, and age. This work lays the foundation for future scientific research and clinical work related to COAD.
{"title":"The Effect of Immune-Related Genes on Colonic Adenocarcinoma Prognosis","authors":"M. Hou, Jiakang Ma, Xiaoyan Lin, Jun Ma","doi":"10.1166/NNL.2020.3209","DOIUrl":"https://doi.org/10.1166/NNL.2020.3209","url":null,"abstract":"Colorectal adenocarcinoma (COAD) is the most common type of colon cancer, and its occurrence is intimately associated with immune-related gene (IRG) expression. In this research, the IRG expression was analyzed and 484 were found significantly different between COAD and normal tissue.\u0000 To construct a COAD patient IRGs-prognosis model, 11 of the IRGs were selected by multivariate Cox analysis. IRGs in the model were closely related to tumor invasion depth, lymphoid involvement, distant metastasis, stage, and age. This work lays the foundation for future scientific research\u0000 and clinical work related to COAD.","PeriodicalId":18871,"journal":{"name":"Nanoscience and Nanotechnology Letters","volume":"12 1","pages":"1055-1062"},"PeriodicalIF":0.0,"publicationDate":"2020-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"45462287","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
The power electronics engineering education is aimed at helping students in becoming qualified electronics engineers. However, the existing evaluation method cannot reflect the students’ performance in knowledge-structure and skillsets objectively and accurately. To address these issues and improve the effectiveness of the current evaluation method in the field, we propose a Bayesian network model-based cognitive diagnostic assessment method and demonstrate it to evaluate students’ knowledge and skills condition with the switched-mode power supply (SMPS) magnetic components design task. The paper starts with a brief introduction to the SMPS inductor design. It continues with the Bayesian network model-based inductor proficiency model, inductor evidence model, and the task model for power magnetics volume and weight in the aerospace SMPS. Then we identify the parameters in the graded response model, relations among variables, and calculate the conditional probability between variables. Finally, we use Markov Chain Monte Carlo estimation method to get the posterior probability distribution of proficiency variables by OpenBUGS. The results show that this cognitive diagnostic assessment system can effectively reflect the students’ study performances, scientifically advise their future study plans, and effectively achieve the education goals.
{"title":"Cognitive Diagnostic Assessment of Power Supply Magnetic Components Design Based on Bayesian Network","authors":"Yi Kuang, Bin Duan, Mengping Lv, Junfeng Wu","doi":"10.1166/NNL.2020.3202","DOIUrl":"https://doi.org/10.1166/NNL.2020.3202","url":null,"abstract":"The power electronics engineering education is aimed at helping students in becoming qualified electronics engineers. However, the existing evaluation method cannot reflect the students’ performance in knowledge-structure and skillsets objectively and accurately. To address these\u0000 issues and improve the effectiveness of the current evaluation method in the field, we propose a Bayesian network model-based cognitive diagnostic assessment method and demonstrate it to evaluate students’ knowledge and skills condition with the switched-mode power supply (SMPS) magnetic\u0000 components design task. The paper starts with a brief introduction to the SMPS inductor design. It continues with the Bayesian network model-based inductor proficiency model, inductor evidence model, and the task model for power magnetics volume and weight in the aerospace SMPS. Then we identify\u0000 the parameters in the graded response model, relations among variables, and calculate the conditional probability between variables. Finally, we use Markov Chain Monte Carlo estimation method to get the posterior probability distribution of proficiency variables by OpenBUGS. The results show\u0000 that this cognitive diagnostic assessment system can effectively reflect the students’ study performances, scientifically advise their future study plans, and effectively achieve the education goals.","PeriodicalId":18871,"journal":{"name":"Nanoscience and Nanotechnology Letters","volume":"12 1","pages":"1044-1053"},"PeriodicalIF":0.0,"publicationDate":"2020-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"42627361","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Lung cancer is a malignant tumor with an extremely high incidence and mortality rate in clinical practice and its pathogenesis remains unclear at present. Currently, the methods for treating this disease have relatively high limitations. However, with the gradual maturity and application of nanotechnology, a number of studies have pointed out that polymethyl methacrylate nanoparticles (PMMA-NPs) encapsulated with curcumin (Cur) possibly becomes a new and effective scheme for treating lung cancer. First of all, Cur-PMMA-NPs were prepared. Their sizes were determined by characterization techniques, and their effects on lung cancer cells A549 were detected by Cell proliferation experiment and flow cytometry. The expression of apoptosis-related proteins in the cells was detected by Western blotting. The results showed that polyacrylic acid (PAA)-Cur-PMMA-NPs had a particle size of (215.00±6.00) nm. The drug loading rate and the encapsulation rate of nanospheres were remarkably higher than those of free Cur (P < 0.05). After the intervention of PAA-Cur-PMMA-NPs in the cells, the cell proliferation and the Bcl-2 expression reduced, while the apoptotic rate and the expression of Bax, Caspase-3, and Caspase-9 increased (P < 0.05). Accordingly, Cur-PMMA-NPs can inhibit lung cancer cells from growth and induce their apoptosis, so they are expected to become an effective intervention measure to improve the therapeutic effect on lung cancer in the future.
{"title":"Effect of Curcumin Coated with Polymethyl Methacrylate Nanoparticles on Lung Cancer Cells","authors":"Ximiao Ma, F. Fu","doi":"10.1166/NNL.2020.3205","DOIUrl":"https://doi.org/10.1166/NNL.2020.3205","url":null,"abstract":"Lung cancer is a malignant tumor with an extremely high incidence and mortality rate in clinical practice and its pathogenesis remains unclear at present. Currently, the methods for treating this disease have relatively high limitations. However, with the gradual maturity and application\u0000 of nanotechnology, a number of studies have pointed out that polymethyl methacrylate nanoparticles (PMMA-NPs) encapsulated with curcumin (Cur) possibly becomes a new and effective scheme for treating lung cancer. First of all, Cur-PMMA-NPs were prepared. Their sizes were determined by characterization\u0000 techniques, and their effects on lung cancer cells A549 were detected by Cell proliferation experiment and flow cytometry. The expression of apoptosis-related proteins in the cells was detected by Western blotting. The results showed that polyacrylic acid (PAA)-Cur-PMMA-NPs had a particle\u0000 size of (215.00±6.00) nm. The drug loading rate and the encapsulation rate of nanospheres were remarkably higher than those of free Cur (P < 0.05). After the intervention of PAA-Cur-PMMA-NPs in the cells, the cell proliferation and the Bcl-2 expression reduced, while the apoptotic\u0000 rate and the expression of Bax, Caspase-3, and Caspase-9 increased (P < 0.05). Accordingly, Cur-PMMA-NPs can inhibit lung cancer cells from growth and induce their apoptosis, so they are expected to become an effective intervention measure to improve the therapeutic effect on lung\u0000 cancer in the future.","PeriodicalId":18871,"journal":{"name":"Nanoscience and Nanotechnology Letters","volume":"12 1","pages":"1015-1021"},"PeriodicalIF":0.0,"publicationDate":"2020-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"45576290","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Barnsley (Barnsley, M.F., 1986. Fractal functions and interpolation. Constr. Approx., 2, pp.303–329) introduced fractal interpolation function (FIF) whose graph is the attractor of an iterated function system (IFS) for describing the data that have an irregular or self-similar structure. Barnsley et al. (Barnsley, M.F., et al., 1989. Recurrent iterated function systems in fractal approximation. Constr. Approx., 5, pp.3–31) generalized FIF in the form of recurrent fractal interpolation function (RFIF) whose graph is the attractor of a recurrent iterated function system (RIFS) to fit data set which is piece-wise self-affine. The primary aim of the present research is investigating the RFIF approach and using it for fitting the piece-wise self-affine data set in ℜ2.
{"title":"Some Results on Recurrent Fractal Interpolation Function","authors":"Wadia Faid Hassan Al-shameri","doi":"10.1166/NNL.2020.3198","DOIUrl":"https://doi.org/10.1166/NNL.2020.3198","url":null,"abstract":"Barnsley (Barnsley, M.F., 1986. Fractal functions and interpolation. Constr. Approx., 2, pp.303–329) introduced fractal interpolation function (FIF) whose graph is the attractor of an iterated function system (IFS) for describing the data that have an irregular or self-similar\u0000 structure. Barnsley et al. (Barnsley, M.F., et al., 1989. Recurrent iterated function systems in fractal approximation. Constr. Approx., 5, pp.3–31) generalized FIF in the form of recurrent fractal interpolation function (RFIF) whose graph is the attractor of a recurrent iterated\u0000 function system (RIFS) to fit data set which is piece-wise self-affine. The primary aim of the present research is investigating the RFIF approach and using it for fitting the piece-wise self-affine data set in ℜ2.","PeriodicalId":18871,"journal":{"name":"Nanoscience and Nanotechnology Letters","volume":"12 1","pages":"1038-1043"},"PeriodicalIF":0.0,"publicationDate":"2020-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"49055669","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
The plug connector is the most widely used type of separable connector; however, the sliding friction that occurs during the plug-in process causes wear and wiping effects. Wear is a form of damage that occurs to the anti-corrosion coating during the sliding process, and wiping refers to the cleaning effect of corrosion products during the sliding process. Both have negative and positive effects on electrical contact reliability in corrosive environments. Therefore, wear and wiping are key factors in studying the life and reliability of connectors in atmospheric corrosion environments. Atmospheric corrosion is the main mechanism leading to connector contact failure. There have been many related studies on simple connector atmospheric corrosion, but research on the influence of wear and wipe on atmospheric corrosion and the influence of contact resistance degradation is still not comprehensive. To study the influence of wear and wiping on contact reliability during the plug connector-mating process, this work evaluates the metal nano-coating connector plugging process as the object, and the contact resistance degradation model under atmospheric corrosion as the basis, and the effect of wear and wiping on corrosion degradation as the research tool. Ultimately, the influence of wear and wiping on contact resistance is studied in detail. Finally, combined with the actual usage of the plug-in connector, the contact reliability of metal nano-coating plug connectors under the action of abrasion and wiping is studied.
{"title":"Study on the Wear and Abrasion Effect of Metal Nano-Coating Plug Connectors Under Atmospheric Corrosion Conditions","authors":"Peng-zhu Wang, Liang Xu","doi":"10.1166/NNL.2020.3203","DOIUrl":"https://doi.org/10.1166/NNL.2020.3203","url":null,"abstract":"The plug connector is the most widely used type of separable connector; however, the sliding friction that occurs during the plug-in process causes wear and wiping effects. Wear is a form of damage that occurs to the anti-corrosion coating during the sliding process, and wiping refers\u0000 to the cleaning effect of corrosion products during the sliding process. Both have negative and positive effects on electrical contact reliability in corrosive environments. Therefore, wear and wiping are key factors in studying the life and reliability of connectors in atmospheric corrosion\u0000 environments. Atmospheric corrosion is the main mechanism leading to connector contact failure. There have been many related studies on simple connector atmospheric corrosion, but research on the influence of wear and wipe on atmospheric corrosion and the influence of contact resistance degradation\u0000 is still not comprehensive. To study the influence of wear and wiping on contact reliability during the plug connector-mating process, this work evaluates the metal nano-coating connector plugging process as the object, and the contact resistance degradation model under atmospheric corrosion\u0000 as the basis, and the effect of wear and wiping on corrosion degradation as the research tool. Ultimately, the influence of wear and wiping on contact resistance is studied in detail. Finally, combined with the actual usage of the plug-in connector, the contact reliability of metal nano-coating\u0000 plug connectors under the action of abrasion and wiping is studied.","PeriodicalId":18871,"journal":{"name":"Nanoscience and Nanotechnology Letters","volume":"12 1","pages":"1006-1014"},"PeriodicalIF":0.0,"publicationDate":"2020-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"43221661","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Shifei Wang, C. Rao, Xin-Qiong Huang, Tianhong Xie, Linling Su, Huan Li
Age-related hearing loss (AHL) is a common, high-incidence, perceptual disease in the elderly population worldwide. Since bisphenol A (BPA) has been reported to associate with cell apoptosis, we hypothesize that BPA can inhibit the neuronal apoptosis in AHL. Forty Wistar rats were recruited to model AHL; they were then treated with different doses of BPA. We used auditory brainstem response testing to measure the BPA-induced improvement in the rats’ hearing. We examined the proliferation and apoptosis of the auditory cortical neurons in the rats with MTT assay and flow cytometry. Also, to delineate the underlying mechanism of BPA’s effect on AHL, we quantitated the expression level of long non-coding RNA X inactive specific transcript (lncRNA XIST) and miR-34a-5p in the rats’ auditory cortex with a novel method called nanoparticle PCR. We found that BPA intervention improved the hearing of AHL model rats, enhanced neuronal cell proliferation, restricted neuronal cell apoptosis, upregulated miR-34a-5p levels, and downregulated lncRNA XIST levels. The dual-luciferase reporter (DLR) assay revealed that BPA inhibited the apoptosis of auditory cortex neurons by targeting miR-34a-5p with lncRNA XIST and regulated the process of AHL. Therefore, we come to a conclusion that BPA contributes to the improvement of AHL, which may be achieved by upregulating miR-34a-5p and inhibiting the apoptosis of auditory cortex neurons via lncRNA XIST.
{"title":"Regulating Age-Related Hearing Loss with Bisphenol A via the Long Non-Coding RNA X Inactive Specific Transcript and MicroRNA-34a-5p Pathways","authors":"Shifei Wang, C. Rao, Xin-Qiong Huang, Tianhong Xie, Linling Su, Huan Li","doi":"10.1166/NNL.2020.3200","DOIUrl":"https://doi.org/10.1166/NNL.2020.3200","url":null,"abstract":"Age-related hearing loss (AHL) is a common, high-incidence, perceptual disease in the elderly population worldwide. Since bisphenol A (BPA) has been reported to associate with cell apoptosis, we hypothesize that BPA can inhibit the neuronal apoptosis in AHL. Forty Wistar rats were recruited\u0000 to model AHL; they were then treated with different doses of BPA. We used auditory brainstem response testing to measure the BPA-induced improvement in the rats’ hearing. We examined the proliferation and apoptosis of the auditory cortical neurons in the rats with MTT assay and flow\u0000 cytometry. Also, to delineate the underlying mechanism of BPA’s effect on AHL, we quantitated the expression level of long non-coding RNA X inactive specific transcript (lncRNA XIST) and miR-34a-5p in the rats’ auditory cortex with a novel method called nanoparticle PCR. We found\u0000 that BPA intervention improved the hearing of AHL model rats, enhanced neuronal cell proliferation, restricted neuronal cell apoptosis, upregulated miR-34a-5p levels, and downregulated lncRNA XIST levels. The dual-luciferase reporter (DLR) assay revealed that BPA inhibited the apoptosis of\u0000 auditory cortex neurons by targeting miR-34a-5p with lncRNA XIST and regulated the process of AHL. Therefore, we come to a conclusion that BPA contributes to the improvement of AHL, which may be achieved by upregulating miR-34a-5p and inhibiting the apoptosis of auditory cortex neurons via\u0000 lncRNA XIST.","PeriodicalId":18871,"journal":{"name":"Nanoscience and Nanotechnology Letters","volume":"12 1","pages":"987-995"},"PeriodicalIF":0.0,"publicationDate":"2020-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"44684606","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Yang Sun, Qianlong Chen, Yujing Zhang, Wenke Li, Kun Zhao, Qingzhi Wang, Hongyue Wang, Zhou Zhou
Considering the lack of information regarding the role and mechanism of low expression of PRKAR1α in cardiac myxoma, we investigated the relationship between the low expression of PRKAR1α and the cAMP responsive element binding protein (CREB) and the expression of its phosphorylated form (p-CREB) in cardiac myxoma tissue. For this purpose, we conducted a retrospective analysis of 130 cases of CM tissue obtained by surgical resection, from which paraffin-fixed tissue DNA was extracted, followed by detection of PRKAR1A DNA mutation by Sanger sequencing and detection of CREB, p-CREB, and PKAR1α protein expression by immunohistochemical SP method. Mutations in the PRKAR1A gene coding region were detected in 35 (46.05%) of 76 sporadic CM tissues, of which 48.57% (17/35 cases) had more than two mutations, and 28.57% (10/35 cases) had exon4: C.349-4-C.349-5insertTmutation. Compared with the surrounding normal tissues, 55.26% (42/76 cases) of PKAR1α protein was not expressed or weakly expressed, CREB was not expressed in myxoma and myocardial tissues, p-CREB was expressed in 51 (67.11%) CM-positive tissues, and PKAR1α and p-CREB expression demonstrated no correlation (P > 0.05). These results indicate the presence of a high level of CREB phosphorylation in cardiac myxoma tissue; however, its phosphorylation is not associated with mutations in the PRKAR1A gene coding region and PKAR1α expression.
{"title":"The Relationship Between cAMP Responsive Element Binding Protein and Its Phosphorylated Form in Cardiac Myxoma with DNA Mutation of Protein Kinase cAMP-Dependent Type I Regulatory Subunit Alpha","authors":"Yang Sun, Qianlong Chen, Yujing Zhang, Wenke Li, Kun Zhao, Qingzhi Wang, Hongyue Wang, Zhou Zhou","doi":"10.1166/NNL.2020.3199","DOIUrl":"https://doi.org/10.1166/NNL.2020.3199","url":null,"abstract":"Considering the lack of information regarding the role and mechanism of low expression of PRKAR1α in cardiac myxoma, we investigated the relationship between the low expression of PRKAR1α and the cAMP responsive element binding protein (CREB) and the\u0000 expression of its phosphorylated form (p-CREB) in cardiac myxoma tissue. For this purpose, we conducted a retrospective analysis of 130 cases of CM tissue obtained by surgical resection, from which paraffin-fixed tissue DNA was extracted, followed by detection of PRKAR1A DNA\u0000 mutation by Sanger sequencing and detection of CREB, p-CREB, and PKAR1α protein expression by immunohistochemical SP method. Mutations in the PRKAR1A gene coding region were detected in 35 (46.05%) of 76 sporadic CM tissues, of which 48.57% (17/35 cases) had more\u0000 than two mutations, and 28.57% (10/35 cases) had exon4: C.349-4-C.349-5insertTmutation. Compared with the surrounding normal tissues, 55.26% (42/76 cases) of PKAR1α protein was not expressed or weakly expressed, CREB was not expressed in myxoma and myocardial tissues, p-CREB\u0000 was expressed in 51 (67.11%) CM-positive tissues, and PKAR1α and p-CREB expression demonstrated no correlation (P > 0.05). These results indicate the presence of a high level of CREB phosphorylation in cardiac myxoma tissue; however, its phosphorylation\u0000 is not associated with mutations in the PRKAR1A gene coding region and PKAR1α expression.","PeriodicalId":18871,"journal":{"name":"Nanoscience and Nanotechnology Letters","volume":"12 1","pages":"979-986"},"PeriodicalIF":0.0,"publicationDate":"2020-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"64673216","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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