Natural Product Research最新文献

Feverfew (Tanacetum parthenium) has been evaluated in multiple clinical studies for migraine management, with earlier meta-analyses suggesting therapeutic potential. Given recent randomised controlled trials (RCTs), this updated systematic review and meta-analysis aimed to assess feverfew's efficacy in reducing migraine frequency, severity, and associated symptoms. A comprehensive search of PubMed, EMBASE, and Google Scholar up to August 2025 identified nine double-masked, placebo-controlled RCTs involving 899 participants. Feverfew significantly reduced migraine attack frequency (IV: -1.11 [-1.23 to -0.99], p < 0.00001; I² = 28%), migraine duration (IV: -4.43 [-7.63, -1.23] at 95% CI, p = 0.007; I² = 98% and showed a non-significant trend towards reduced pain severity (IV: -0.63 [-1.48 to 0.21], p = 0.14; I² = 85%). No significant effects were observed on migraine associated symptoms such as nausea, vomiting, photophobia, and phonophobia. Moderate heterogeneity, risks of selection, and attrition bias limited the overall quality of the evidence. Feverfew may offer modest benefits in migraine prevention, warranting further high-quality, large-scale RCTs with standardised protocols.

The stems of Dendrobium chrysanthum Lindl. yielded three previously undescribed compounds, namely 2-(5-methyl-6-ethoxyphenyl)-flavone (1), a flavonoid; 3,12-dioxo-19-methoxy-friedelane (2), a triterpenoid; and (2'-methylpiperidine-7-ethenyl ester)-cinnamic acid (3), an alkaloid. Additionally, seven previously known compounds are newly recorded in this species, including phenylpropanoids (4), fatty acid derivatives (5), triterpenoids (6 and 7), and steroidal saponins (8-10). Structural elucidation of the new compounds was achieved through extensive spectroscopic techniques, including HR-ESI-MS,¹H and ¹³C-NMR, HSQC, and HMBC, supplemented by comparison with literature data. These findings enhance the phytochemical understanding of D. chrysanthum Lindl. and support its potential as a source of bioactive and structurally diverse natural products.

Fomitopsis betulina (birch polypore) is a medicinal polypore with limited biochemical characterisation. This study provides the first evidence of polyamines and a detailed phenolic profile in the 80% methanolic (MeOH) extract of its fruiting bodies. HPLC analysis revealed spermidine (60.21 ± 0.97 mg/kg d.w.), spermine (41.71 ± 2.56 mg/kg d.w.), and putrescine (0.97 ± 0.10 mg/kg d.w.), with spermidine as the predominant form. Gallic acid (14,415.07 ± 1,297.36 ng/g d.e.) and quinic acid (11,332.40 ± 1,133.24 ng/g d.e.) were accompanied by apiin (514.81 ± 25.74 ng/g d.e.), reported here for the first time in this species. The MeOH extract exhibited notable antioxidant capacity (DPPH: 2.99 ± 0.11 mM TE/g; ABTS: 63.50 ± 0.51 mM TE/g) and strong acetylcholinesterase inhibition (99.03 ± 5.52%). These findings highlight F. betulina as a source of polyamines and phenolics with promising antioxidant and neuroprotective potential.

Datura, a widely cultivated plant of the Solanaceae family, is well known for its medicinal properties. Among the various Datura species found globally, Datura metel L. and Datura stramonium L. have garnered significant research interest due to their historical transoceanic connections between the Old and New World. The alkaloids present in Datura exhibit notable ethnobotanical and medicinal properties. This study employs chemoinformatics analysis on 46 and 49 screened alkaloids from D. metel and D. stramonium, respectively, to explore their chemical space, scaffold diversity, protein target interactions, and toxicity profiles. Additionally, chemical similarity analysis with FDA-approved drugs identified 10 alkaloids, predominantly belonging to the tropane-class, with potential drug-like properties. The scaffold 'N1C2CCCC1CC2' emerged as a key structural motif with promising lead-like characteristics for future drug-design efforts. Modifying-enzymes and transmembrane signal-receptors are major interacting proteins, with pathway analysis underscoring their neuromodulatory role. These findings pave the way for their application in drug-discovery.

A new monosubstituted phenol, Diaphenol A (1), along with nine related analogues, 2-phenylethanol (2), (4-methoxyphenyl)acetic acid (3), 3-hydroxybenzyl alcohol (4), 1-phenyl-1,2-ethanediol (5), bungein A (6), 2-(4-hydroxyphenyl)ethyl acetate (7), hydroxyphenylacetic acid (8), tyrosol (9), and orcinol (10) were isolated from the mangrove-derived fungus Diaporthe spinosa ZMU-32-1. The chemical structures of these compounds were elucidated through comprehensive spectroscopic analysis, including 1D and 2D NMR and high-resolution electrospray ionisation mass spectrometry (HR-ESIMS), and by comparison with previously reported spectral data. The antifungal activity of compounds 1-10 was evaluated, among which compound 6 exhibited notable antifungal activity against Bipolaris sorokiniana, with a minimum inhibitory concentration (MIC) value of 80 μg/mL.

Beyond the extract, which protects the intestine via ROS scavenging and inhibition of JAK/STAT and JNK pathways, this study examines the anti-parasitic activity of Hylotelephium erythrostictum (Miq.) H. Ohba polysaccharides (HEPs). HEPs were isolated from H. erythrostictum (Miq.) H. Ohba via optimised hot-water extraction, Sevage deproteinization, and dialysis. Monosaccharide composition revealed HEPs are heteropolymers (Rha:Ara:Gal:Glc:GulUA ≈ 1.0:1.8:2.1:3.5:3.2), dominated by glucose (≈32 mol%) and unprecedented guluronic acid (GulUA, ≈28 mol%. SEC-MALLS-RI showed weight-average Mw = 37.78 kDa, number-average Mn = 5.20 kDa, indicating a bimodal distribution (≈38 kDa GulUA-rich and ≈5 kDa fractions). The lack of therapeutic selectivity in HEPs, due to their low selectivity index (SI ≈ 0.85), contrasts with their protective potency, which is comparable to the standard drug. These findings elucidate a unique architecture whose therapeutic potential against toxoplasmosis may be realised upon addressing the issue of low selectivity.

Lindera glauca, an edible medicinal plant native to Northeastern Asia, has been reported to possess various pharmacological activities. This study employed cell extraction (CE) in conjunction with LC-QTOF-MS/MS to identify bioactive components that bind to target cells. The 80% MeOH extract of L. glauca exhibited significant nitric oxide (NO) production inhibitory activity in BV2 cells and was applied to the CE coupled with the LC-QTOF MS/MS method. New compound 3,5-dimethoxyphenyl-4-hydroxymethyl-β-ᴅ-glucopyranoside (2), and five known compounds (1, 3-6) were isolated from L. glauca. All compounds displayed anti-inflammatory activities, and compounds 2-3 inhibited NO production in BV2 cells. In molecular docking simulations, compound 2 showed high binding affinities to inflammatory marker proteins COX-2 and iNOS. In conclusion, the CE method combined with LC-QTOF MS/MS effectively facilitates the rapid identification of potential active compounds. And new compound 2 from L. glauca may be a new candidate against inflammatory diseases.

Phytochemical investigation of the bark of Lannea acida led to the isolation of a new flavonolignan glycoside, mururin A-3-O-α-L-rhamnopyranoside (1), along with twenty-three known secondary metabolites compounds 2-24. The structures of these compounds were determined using spectroscopic methods, including NMR and mass spectroscopy. Compounds 1 and 2 displayed good inhibitory effects against LmPTR1 with IC₅₀ values of 56.43 ± 2.14 and 104.6 ± 3.30 μM, respectively, as compared to the positive control trimethoprim (IC₅₀ = 21.07 ± 0.6 μM). Molecular docking and molecular dynamics simulations were performed to explore the binding mechanisms and stability of PTR1-inhibitor complexes over the timeframe of 100 ns. Notably, all evaluated compounds demonstrated no significant cytotoxicity against the human normal fibroblast cell line (BJ). This study reveals the significant anti-parasitic effects of flavonolignans compounds 1 and 2 against Leishmania species, the parasites responsible for leishmaniasis - a group of clinically important tropical diseases.

Phytochemicals, particularly polyphenols, play a crucial role in modern healthcare due to their preventive and therapeutic properties. Among these, epigallocatechin-3-gallate (EGCG), a potent catechin found in green tea, exhibits exceptional antioxidant, anti-inflammatory, and disease-modulating effects, making it a promising candidate for managing cancer, diabetes, and cardiovascular disorders. However, its clinical translation is hindered by instability and poor bioavailability. Recent advancements in nanotechnology have addressed these limitations through innovative nano-formulations that enhance encapsulation, stability, and targeted delivery, thereby improving therapeutic efficacy. This review comprehensively examines the latest research on EGCG's health benefits, its pharmacokinetic challenges, and the development of nanomaterial-based delivery systems to maximise its biomedical potential. By critically evaluating current strategies and future directions, this work highlights the transformative role of nano-engineered EGCG in bridging the gap between preclinical promise and clinical application.

Lindera obtusiloba Blume (LO; family Lauraceae) has been shown to possess various bioactive properties, but LO wood essential oil (LOWEO) potential for skin whitening or anti-hyperpigmentation remains unexplored. Here, we explored the effects of LOWEO on biological activities associated with skin whitening in B16BL6 melanoma cells. LOWEO was prepared via steam distillation, revealing 27 components via GC/MS. LOWEO attenuated serum-induced B16BL6 cell proliferation. Furthermore, in α-MSH-stimulated B16BL6 cells, LOWEO decreased melanin synthesis and tyrosinase activity, along with the downregulation of microphthalmia-associated transcription factor, tyrosinase, and tyrosinase-related protein-2 protein expression. LOWEO also enhanced the phosphorylations of ERK1/2 and P38 MAPK, whereas it suppressed JNK phosphorylation and reduced melanophilin and Rab27a protein expression in these cells. These results suggest that LOWEO may exert skin-whitening and anti-hyperpigmentation effects by suppressing melanin production and interfering with melanosome transport in B16BL6 cells. Therefore, LOWEO should be considered a promising developmental starting point for natural agents that alleviate and/or prevent skin hyperpigmentation.