Natural Product Research最新文献

The aim of the present study was to demonstrate the phytochemical characterisation, antioxidant and antimicrobial properties of methanol extracts of leaf parts of A. nilagirica. The Phytochemical characterisation by GC-MS analysis revealed the presence of 30 phytoconstituents such as Methyl commate A (14.69%), Phytol (13.91%), 8,9-Dihydro-9- vinyl-cycloisolongifoline (7.86%), Neophytadine (6.18%), Alpha- Amyrin (5.52%) and Squalene (4.99%), 9-Acetyl-S-octahydrophenanthrene (4.62%), Olean-12-en-3-one (3.70%), Acetyloxyparthenin (3.60%) as the main components. For antioxidant activity, the leaf extract demonstrates IC₅₀ value 8.80 ± 0.03 µg/ml using DPPH, 30.21 ± 0.1 µg/ml using MCA, and 7.62 ± 0.01 µg/ml using FRAP scavenging assay respectively. The anti-bacterial potential of methanol extract against the 2 Gram-negative and 2 Gram-positive pathogenic bacterial strains show that extract has significant inhibition potential with mean inhibition zone in the range of 16 to 20 mm with MIC values 31.25 to 125 µg/ml. For quantitative phytochemical analysis, the highest Total Phenolic Content (TPC), 372.3 mg GAE/g, Total Flavonoid Content (TFC-1), 287.35 mg QE/g and Total Flavonol Content (TFC-2), 138.43 mg QE/g of dry extract were found for methanol extract respectively.

Cuphea carthagenensis is known for its potential antioxidant and anti-inflammatory properties, attributed to triterpenes, flavonoids, and tannins. This study aimed to optimise the extraction process for aerial parts of C. carthagenensis using vortex extraction. A Box-Behnken experimental design coupled with response surface methodology was employed to evaluate the effects of three independent variables: sample-to-solvent ratio, velocity, and temperature. The optimised extract underwent phytochemical screening and assessment of its antimicrobial capacity. The highest extraction yields were obtained through maceration (22.84%) and ultrasound-assisted extraction (21.95%). However, the extract by vortex extraction exhibited significantly higher phenolic content (250.17 µg_EAG g_ext^-1) and antioxidant capacity. The optimal extraction conditions were identified as a sample-to-solvent ratio of 1:35, 12,000 rpm, and a temperature of 60 °C. The optimised extract was characterised by a predominance of glycosylated flavonoids and demonstrated moderate antibacterial activity, particularly against Streptococcus pyogenes, Staphylococcus epidermidis, and Bacillus subtilis.

Twenty-one compounds were isolated and identified from the ethanol extract of Viscum coloratum (Kom.) Nakai, including two new compounds (1 and 3), a new artificial product (2) and eighteen known compounds (4-21). Compounds 4-9 and 11-20 were isolated from Santalaceae for the first time. In this paper, compounds' structures identified by NMR spectroscopy and compare their data with those in the literature. Meanwhile, all compounds were assessed for cytotoxic activity against HepG2 cells, revealing that compound 1 exhibited significant cytotoxicity with IC₅₀ value 6.70 ± 2.12 μM.

Aristolochia gigantea (Aristolochiaceae), commonly known as the Brazilian Dutchman's pipe, is cultivated globally as an ornamental vine, admired for the unique shape of its large flowers. In our ongoing research into the chemical composition of A. gigantea, the ethanol extract from its seeds was fractionated using chromatographic techniques, resulting in the isolation and identification of allantoin, along with the lignans kobusin, eudesmin, and methylarctigenin. The fractions were further analysed by NMR and GC - EI/MS, employing a molecular networking approach and retention index comparison, which led to the annotation of four sterols, eleven aliphatic alcohols, one saturated fatty acid, two unsaturated fatty acids, ten esters of saturated fatty acids, ten esters of unsaturated fatty acids, and one lactone.

Glisoprenins are unique fungal polyisoprenepolyols with nine isoprene units from Gliocladium spp. Herein, glisoprenin G (1), a new member of glisoprenins group, along with a biosynthetically related known analogue glisoprenin F (2), were isolated and identified from an endophytic fungus, Gliocladium sp. F1, harboured in the roots of the Chinese medicinal plant Mahonia fortunei. The chemical structure of 1 showed the unusual loss of two isoprene carbon atoms and was determined by HRMS and NMR data. Compounds 1 and 2 showed moderate cytotoxic activity.

α-Glucosidase inhibitory assay-guided isolation of the aqueous extract from Cannabis sativa leaves afforded three new compounds named cannabisaldehyde (8), cannacone A (9), and canniprene C (10), along with eight previously known compounds (1-7, 11). The structures of new compounds were determined through extensive analysis of various spectroscopic data. Of isolated compounds, cannacone A (9) demonstrated most potent inhibition against maltase and sucrase with IC₅₀ values of 80.0 and 82.9 μM, respectively. Cannacone A (9) inhibited both maltase and sucrase by competitive mechanism.

Two new phytoecdysteroids, (24Z)-20,26-dihydroxy-24(28)-dehydro-28-methyl-ecdysone (1), (24Z)-22,25-epoxy-2,3,14,20-tetrahydroxyergosta-7,24(28)-dien-28-methyl-6-one (2), together with eleven known compounds (3 - 13) were isolated from the whole plant of Tournefortia sibirica L. The chemical structures of the compounds were elucidated by 1D and 2D NMR and HR-ESI-MS and compared with data from the literature. The isolated compounds were evaluated for their inhibitory effects on NO, TNF-α and IL-6 production in LPS-induced RAW 264.7 cells. Among them, compounds 6, 7, 9, and 10 showed anti-inflammatory effects against NO, TNF-α, and IL-6 levels with IC₅₀ values ranging from 30.44 ± 2.10 to 66.88 ± 2.32 μM.

Orientin is one of the flavonoid glycosides with diverse biological properties such as anticancer, antioxidant, neuroprotective, cardioprotective, antiallergic, and anti-inflammatory. It is found in several plants like rooibos tea, Ocimum sanctum, Trollius, Passiflora, and Phyllostachys species. This review aimed to summarise the various medicinal properties of the orientin focusing on its underlying molecular mechanism reported based on in-vitro and in-vivo studies. The data were collected using various search engines, incorporating PubMed/Medline, Scopus, Science Direct, Google Scholar, Web of Science, and SpringerLink. Our findings showed that orientin exhibited promising anticancer, neuroprotective, anti-inflammatory, and antioxidant activities. Hopefully, this information could assist drug researchers and pharmaceutical entities in finding an effective herbal drug for the treatment of different disorders with potential mechanisms of action. Meanwhile, further investigations are warranted such as oral bioavailability, pharmacokinetics and pharmacodynamic characteristics of orientin to establish fully drug profiling suitable for clinical trials.

The chloroform extract of leaves of E. dendroides L, reduced the levels of lipid profile in rats with hypercholesterolaemia to near-normal levels. Additionally, it significantly decreased the amount of malondialdehyde (MDA). In addition, the extract augmented the levels of superoxide dismutase (SOD) and glutathione peroxidase (GSHPx) in the hypercholesterolemic treated rats. The docking results verified the binding mechanism of isolated compound as HMG-CoA reductase inhibitor. The identified compound has the essential pharmacophoric features of Simvastatin. Furthermore, the ADMET profile of an isolated terpene was computed and compared to that of simvastatin as a reference medication. This compound demonstrated a favourable absorption rate of 90.44, which is similar to that of simvastatin (94. 63). Additionally, it demonstrated better CNS penetration and lower excretion rates than simvastatin, resulting in a longer duration of action. It also has an extensive therapeutic window and better selectivity towards cancer cells in opposition to normal cells.

This study aimed to evaluate the antihyperglycemic and antioxidant activities of the lectin isolated from Alsidium triquetrum (BTL). Diabetes was induced in Wistar rats through low-dose streptozotocin injections. Following the confirmation of hyperglycaemia, the animals were treated with 150 mM NaCl, glibenclamide, or BTL at 600 or 900 mg/kg. The efficacy of BTL treatment was assessed by measuring serum glucose, total cholesterol, triglycerides, glutathione peroxidase, catalase, and superoxide dismutase levels on days 7, 14, and 21 post-induction. On day 21, pancreas and liver tissues were excised for histopathological analysis. BTL significantly reduced serum glucose, total cholesterol, and triglyceride levels while increasing all antioxidant enzyme levels. Moreover, the histopathological analysis suggests that BTL exerts a protective effect against diabetes-induced damage in hepatic and pancreatic tissues, indicating its potential as a therapeutic agent in managing diabetes.