Natural Product Research最新文献

Burnatia enneandra was traditionally used across the world for medicinal purposes. In Cameroon, this plant treats injuries, stomach aches and some intestinal parasites. Therefore, the bioactive molecules responsible for these potentials and their mechanisms of action remain unknown. This study investigates, for the first time, the antibacterial effects of isolated compounds from B. enneandra and their effective use for therapeutic purposes without side effects. The bioactive molecules of B. enneandra were obtained from ethyl acetate extract (EA.E) by applying various chromatographic methods. The structures were elucidated using HR-ESI-MS and 1D- & 2D-NMR spectroscopic data in addition to literature. The antibacterial activity was evaluated on four bacteria strains. As a results, one new diterpenoid of the clerodane (1) type, together with five known diterpenoids (2, 3, 4, 5, 6), was isolated in the EA.E of B. enneandra tubers. All tested negative-gram bacteria strains showed high MIC against isolated compound (1) at 0.04 µmol/mL.

An automated medium-pressure liquid chromatography (MPLC) system was employed for the preparative-scale isolation of lupane-type triterpenoids from Glochidion zeylanicum var. tomentosum (Dalzell) Trimen. Seven compounds were isolated and identified as glochidone (1), glochidonol (2), glochidiol (3), lupeol (4), epilupeol (5), lup-20(29)-ene-3α,23-diol (6) and lup-20(29)-ene-1α,3β-diol (7). The antimicrobial activity of these compounds was evaluated against Micrococcus luteus, Staphylococcus aureus, Aspergillus fumigatus and Rhizopus oryzae using the minimum inhibitory concentration (MIC) method. Glochidiol (3) showed moderate activity against S. aureus (64 µg/mL) and R. oryzae (32 µg/mL). Compound 6 also showed moderate antifungal activity against A. fumigatus (64 µg/mL). The results highlight the efficiency of MPLC and the influence of hydroxyl substitution on antimicrobial activity.

To clarify the cytotoxic potential of Mesua caudata (King) Kosterm., three novel benzophenone derivatives, mesucaudatins A-C (1-3), along with one known xanthone compound (4), were found in the stem bark of this plant. The structure determination of all benzophenones (1-3) was carried out by 1D (¹H,¹³C) or 2D (HMQC, HMBC) NMR spectroscopy, and high-resolution MS data. All isolates from this plant were first tested for cytotoxicity against cervical (HeLa) and breast cancer (MCF-7) cells. Mesucaudatin A (1) exhibited potent cytotoxic activity against HeLa cells and MCF-7 cells with IC₅₀ values of 0.76 and 9.29 μM, respectively. Macluraxanthone (4) also showed high activity against HeLa cells (IC₅₀ = 3.96 μM) and moderate activity against MCF-7 cells (IC₅₀ = 10.10 μM).

Koenigia mollis, an underexplored traditional medicinal plant (MP) from Nagaland, was investigated for its phytochemical composition with emphasis on characterisation of bioactive constituents. Metabolic profiling was conducted using preliminary phytochemical screening, Fourier Transform Infra-red Spectroscopy (FT-IR) and Gas Chromatography-Mass Spectrometry (GC-MS). GC-MS enabled tentative identification of 45 phytochemical (PC) compounds through spectral comparison with reference databases, while FT-IR analysis revealed functional groups supporting structural elucidation. Preliminary screening confirmed the presence of major phytochemical classes such as alkaloids, flavonoids, terpenoids etc. In silico tools were employed where screened compounds exhibited pharmacokinetic properties, drug-likeness and potential biological activities of identified compounds. This study highlights the chemical diversity of Koenigia mollis and provides foundational phytochemical data, contributing valuable insights to the limited knowledge of underexplored medicinal plants and underscoring its medicinal potential.

A new flavonol glycoside, patuletin 3-O-β-D-glucopyranosyl-(1→6)-β-D-glucopyranoside-7-O-β-D-glucopyranoside (1), together with three known flavonol glycosides (2-4) were isolated from Eriocaulon buergerianum Körn. Their structures were illuminated by comprehensive spectral methods and chemical methods. Molecular docking and deep learning studies showed that these compounds have potential α-glucosidase inhibitory activity. Α-glucosidase inhibition assay showed that the IC₅₀ values of four flavonol glycosides (1-4), along with two aglycones, patuletin (5) and quercetin (6), were 205.2, 169.8, 508.5, 547.3, 57.0 and 14.5 μM, respectively. The acid hydrolysis experiment of the aqueous extract indicated that the flavonoids in this plant are mainly flavonol glycosides containing patuletin. Therefore, the flavonoids containing patuletin in this plant may be important constituents for its hypoglycaemic activity.

The complex pathophysiology of Alzheimer's disease underscores the need for potent AChE inhibitors. In this context, we investigated, for the first time, the acetone extracts of three Algerian medicinal plants: Anvillea garcinii subsp. radiata (Coss. & Durieu) Anderb., Marrubium deserti (de Noé) Coss., and Asphodelus tenuifolius Cav., through combined in vitro AChE assays, LC-MS/MS metabolite profiling and in silico evaluation. Total phenolic and flavonoid content analyses revealed that M. deserti had the highest levels TPC/TFC (209.8 ± 0.6 mgGAE/g and 161.0 ± 0.9 mgQE/g, respectively). In vitro AChE inhibition assays, showed that galantamine exhibited the strongest activity (4.23 ± 0.02 μg/mL) followed by the A. garcinii subsp. radiata (IC₅₀=122.88 ± 1.30 µg/mL). LC-MS/MS profiling identified 21 compounds, with A. garcinii subsp. radiata displaying the most diverse chemical profile. Molecular docking analysis revealed strong binding affinities, with hesperidin exhibiting the highest binding energy (-11.2 kcal/mol), comparable to the reference drug donepezil (-11.5 kcal/mol).

Two new compounds, (1S,5S,7R,10S)-10-hydroxyl-deoxysecoatractylo-δ-lactone-11-O-β-D-glucopyranoside (1) and (3 R,5S,10S)-Atracty-lenolide I-3-O-β-D-apiopyranosyl(1→6)-β-D-glucopyranoside (2), were isolated from the CH₂Cl₂ extract of Atractylodes japonica Koidz. ex Kitam., along with fifteen known compounds (3-17). Their structures were elucidated by 1D NMR spectroscopy (¹H-NMR,¹³C-NMR), 2D NMR experiments (HMBC, HMQC, ¹H-¹H COSY, NOESY), together with high-resolution mass spectrometry. The two compounds exhibited cytotoxicity against the HepG-2 cancer cell line, with IC₅₀ values of 44.34 ± 1.79 and 46.27 ± 1.46 μM, respectively. In addition, compound 1 was evaluated for its cytotoxic activity against the A549 and HeLa cell lines, with IC₅₀ values of 30.54 ± 1.58 and 36.73 ± 1.19 μM, respectively; compound 2 showed strong effects against the BGC-823 and HT-29 cell lines, with IC₅₀ values of 32.53 ± 1.13 and 35.16 ± 1.78 μM, respectively.