Natural Product Research最新文献

Wogonin, a flavonoid derived from the plant Scutellaria baicalinase's, has emerged as a promising candidate for sepsis management due to its multifaceted pharmacological properties. A comprehensive search of databases, including Google Scholar, Scopus, Web of Science, PubMed, and Embase, was conducted up to November 2025 using relevant keywords. In-vivo studies were assessed using the SYRCLE risk of bias tool, and in-vitro studies were evaluated with the OHAT risk of bias tool. A total of 449 articles were initially sourced, ultimately, 24 publications included in this systematic review. Evidence from various in-vivo and in-vitro studies indicates that wogonin exerts protective effects against sepsis-induced organ damage by modulating critical signalling pathways such as NF-κB, MAPK, and Nrf2/HO-1. Despite these promising findings, further clinical trials are necessary to establish the safety and efficacy of wogonin in human subjects suffering from sepsis.

A phytochemical investigation of Callicarpa integerrima resulted in the isolation of two previously undescribed clerodane diterpenoids, compounds 1 and 2, in addition to four known compounds (3-6). Structural determination of the new compounds was accomplished through a comprehensive investigation of their spectral data (1D and 2D NMR, HR-ESI-MS and UV/IR), and their absolute configurations were assigned by the comparison of the experimental and calculated ECD curves, supported by specific rotation data. LDH release in J774A.1 macrophages was used to evaluate the anti-inflammatory effects of compounds 1-6. Compound 2 revealed remarkable activity, with an IC₅₀ of 5.12 ± 0.24 µM, compared to andrographolide (IC₅₀ of 12.48 ± 0.60 µM), effectively inhibiting lipopolysaccharide and nigericin-induced pyroptosis in a dose-dependent manner, highlighting its potential as a promising NLRP3 inflammasome inhibitor for further development.

This study details the biochemical profiling of leaf, fruit, and seed extracts from Alangium salviifolium (L.f.) Wangerin (Cornaceae). Qualitative screening detected alkaloids, phenolics, flavonoids, tannins, saponins, glycosides, terpenoids, sugars, coumarins, proteins and amino acids. Quantitative assays revealed seeds with highest flavonoids (98.26 mg RE/g) and phenolics (72.42 mg GAE/g), fruits richest in alkaloids (69.46 mg AE/g) and leaves abundant in tannins (60.34 mg TAE/g) and phenolics (65.40 mg GAE/g). UV-Vis, FTIR and HPLC analyses highlighted organ-specific differences. Extracts showed dose-dependent cytotoxicity against A431 cells, with IC₅₀ values of 10, 8 and 6.8 µg/mL for leaf, fruit and seed, respectively-seed extract nearing doxorubicin (5.4 µg/mL). LC-MS identified conserved C18 unsaturated fatty acids (m/z 277-281), phenolics/glycosides (m/z 227-323) and escalating lipid fragments (m/z 455-610) from leaves to fruit/seed. These findings confirm anticancer potential, warranting isolation of active compounds for mechanistic studies and drug development.

A new triterpenoid saponin, 3β,16β,23α,28β-tetrahydroxyoleana-11,13(18)- dien-30β-oic acid-3-[β-D-glucopyranosyl-(1→2)]-[β-D-glucopyranosyl-(1→3)]-β-D- fucopyranoside, which was named clino-chinsaponin A (1), together with three known compounds, namely clinoposaponin D (2), naringenin (3), and narirutin (4), were isolated from Clinopodium chinense (Benth.) Kuntze. The structural assignments for the isolated compounds were elucidated by analysis of NMR data, and by comparison of the acquired spectroscopic data with values reported in the literature. Moreover, compounds 1, 2, and 4 were tested against five bacterial strains (Gram-positive and Gram-negative). Among them, compound 1 showed weak antibacterial activity against Pseudomonas aeruginosa.

Three new flavonol compounds-kushenmin Q (1), kushenmin R (2), and kushenmin S (3)-along with two known compounds, 2S-sophoflavonoid B (4) and kushenol G (5), were isolated from the ethanol extract of Sophora flavescens root bark. Their structures were elucidated using UV spectroscopy, high-resolution electrospray ionisation mass spectrometry (HRESIMS), nuclear magnetic resonance (NMR), and comparison with published data. The antibacterial activity of compounds 1-5 was evaluated against eight bacterial strains (four Gram-positive and four Gram-negative) using the microbroth dilution method. Among the isolated compounds, the flavones 1, 3, and 4 exhibited significant antibacterial activity, with particularly strong effects against Gram-positive bacteria.

Inflammatory bowel disease imposes significant healthcare burdens, driving the search for natural therapeutic agents. This study investigated the protective effects of insect gall Methanol extract from Picea koraiensis Nakai (PK) against colitis. Using in vitro (H₂O₂-induced IEC-6 enterocytes) and in vivo (DSS-induced murine colitis) models, we assessed PK's impact on oxidative stress, inflammation, and apoptosis. In vitro, PK (0.6 mg/mL) significantly enhanced cell viability (∼40%) and key antioxidant enzyme activities, while reducing oxidative markers (MDA, LDH, ROS) by over 50%. It also suppressed apoptosis via caspase-3/9 inhibition and activated the Nrf2/HO-1 pathway. In vivo, PK ameliorated colitis severity, improved histopathology. Transcriptomic and molecular analyses confirmed the activation of the Nrf2/HO-1 pathway and inhibition of p53/p38-mediated apoptosis in colonic tissue. In conclusion, PK exhibits multimodal protection against colitis by alleviating oxidative stress, inflammation, and apoptosis, primarily via Nrf2/HO-1 activation. Future studies should focus on its pharmacokinetics and long-term safety.

This study used UPLC-QE plus Orbitrap-MS to analyse metabolomic differences between Acanthopanax senticosus fruit (AHF) and Acanthopanax sessiliflorus fruit (ASF). 79 compounds were identified in AHF and 69 in ASF, with 47 shared. 1,282 differential factors (74 significant) involved 13 core pathways like phenylalanine metabolism, with key metabolites L-phenylalanine and kaempferol. via metabolomics and bioactivity evaluation, we revealed distinct differences in composition, metabolic mechanisms and bioactivities. AHF focuses on basal metabolism, showing cardiovascular protective advantages by regulating phenylalanine metabolism, synthesising chlorogenic acid, and inhibiting ACE/α-Ease activities. ASF centres on secondary metabolism: Eliminate photoreactive oxygen species to mitigate membrane lipid peroxidation. These metabolites synergistically activate the flavonoid pathway, maintaining membrane stability and high CAT/SOD/POD activities, enhancing antioxidant capacity. Future research may explore its potential applications in pharmaceuticals and functional foods through clinical trials.