Natural Product Research最新文献

The present work investigated the anxiolytic effect of the alkaloid fraction (AF II) from the root bark of Mimosa tenuiflora. Female Swiss mice of 6 weeks old were used in this study. The animals were divided into three groups: 0.9% NaCl (vehicle group, s.c.), AF II (6 mg/kg, s.c.), and diazepam - DZP (5 mg/kg, s.c.). The light/dark box and elevated plus maze tests evaluated the animal anxiety. DMT was identified by the HPLC method. AF II showed significant anxiolytic effects in the two behavioural tests used.

Gliomas, the most devastating of brain tumours, pose immense therapeutic challenges due to the adverse effects of standard chemotherapeutic drugs. Seeking alternatives, natural products have emerged as promising sources for cancer treatment. Vitex negundo, a significant medicinal plant in traditional medicine, offers potential remedies for various ailments. In this study, fractionation via column chromatography we isolated fraction #25 from leaves of Vitex negundo. Employing 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay, wound healing assay, flow cytometry for cell cycle analysis, and HRLC-MS for compound identification, we investigated its effects on glioma cells. Results indicate that fraction #25 significantly reduces glioma cell viability and proliferation, inhibits cell migration in a dose-dependent manner, and arrests the cell cycle at G1 phase. Compound analysis reveals the presence of potent antiproliferative agents, including 7-hydroxy-3,4,5,6,8-pentamethoxyflavone, Virol-B, Momordin Ia, and Oryzalexin A. These findings underscore the potential of Vitex negundo as a source of anticancer compounds against glioma cells.

One new meroterpene derivative, millmerranones G (1), and three known analogues (2-4) were identified from the mangrove-derived fungus Aspergillus sp. GXIMD 03004, which was isolated from the leaves of mangrove Acanthus ilicifolius L. collected from Beibu Gulf in China. The structure of 1 was characterised by a comprehensive interpretation of the NMR spectroscopic and HRESIMS data. The absolute configuration for 1 was established using experimental and calculated ECD data. The anti-Vibrio activities of all compounds were evaluated, the result showed that compounds 1 and 2 has weak activity against Vibrio harveyi.

Syzygium aromaticum L. (clove) is a species native to subtropical countries. Its dried flower buds are rich in essential oils, which have shown insecticidal, anti-inflammatory and anaesthetic effects. This work was aimed to study the differences in antioxidant and anticancer activities between clove essential oil (CEO) and its major component, eugenol. The chemical composition of the CEO was determined by GC-MS. The physicochemical properties and antioxidant activity were determined in CEO and eugenol. Finally, anticancer activities were assayed against seven cell lines. Chemical analysis revealed that 80% of the CEO was eugenol. The density and IR were similar, and the colour was ΔE*>3. CEO had a lower phenolic content, but similar antioxidant activity to eugenol. The anticancer activity of the CEO was greater than that of eugenol in all the cell lines except for HeLa cells. These results suggest that secondary compounds in CEO enhance its antioxidant and -anticancer activities.

Monnieriside A (MoA) is a chromone isolated from Evolvulus linarioides. This study investigated the antinociceptive potential of MoA in mice. MoA (0.01-100 mg/kg, i.p.) inhibited nociception in the inflammatory phase of the formalin test without causing motor impairment. MoA (0.1-100 mg/kg, i.p.) also reduced hindpaw mechanical allodynia caused by either intraplantar injection of Complete Freund's Adjuvant (CFA) or surgical paw incision to simulate postoperative pain. Postoperative antinociception was accompanied by reduced IL-1β levels in the incised paw, assessed by ELISA. The antinociceptive action of MoA (100 mg/kg, i.p.) was preserved in IL-10 knockout mice submitted to paw incision, indicating that IL-10 is not essential to the antinociceptive effect. Interestingly, MoA (100 mg/kg, i.p.) increased the expression of TGF-β in IL-10 knockout mice, which could be a compensation mechanism leading to an antinociceptive effect in the absence of IL-10.

Penicillium species are renowned for their ability to produce a wide range of secondary metabolites with medicinal properties. In this study, compounds 1-10 were isolated from Penicillium sp. Z-16, of which compound 1 is a new benzophenone derivative named methyl 2-(2,6-dihydroxy-4-methylbenzoyl)-4,5-dihydroxy-3-methoxybenzoate. The chemical structure of 1 was determined through comprehensive spectroscopic analysis, including 1D, 2D NMR (HMBC, HSQC) and HRESIMS. In addition, six other known compounds (11-16) were isolated and identified from Penicillium sp. T-5-1. The antimicrobial activity tests demonstrated that compound 1 was moderately active against Candida albicans with a MIC value of 125 μg/mL, while compound 2 showed a MIC value of 62.5 μg/mL against Staphylococcus aureus.