Natural Product Research最新文献

Several breakdown products, including isothiocyanates (ITCs) are formed by hydrolysis of naturally occurring secondary metabolites in Brassica plants. These compounds are known to exert positive effects on health. The present study aimed to detect the presence of these volatile compounds in the raw and fermented Kohlrabi and optimise and validate a HPLC-DAD method for quantification of erucin and phenethyl isothiocyanate in Kohlrabi. Correlation coefficients of 0.979 and 0.978 were attained over the range 1-20 μg/ml and 0.5-10 mg/ml for erucin and phenethyl isothiocyanate, respectively, The LOD and LOQ values for erucin and phenethyl isothiocyanate were 3.36, 10.18 μg/ml and 1.73, 5.26 mg/ml, respectively. The accuracy for the analysis was confirmed by the recovery values of 97-98%. The validated method was used to analyse these two compounds in raw and fermented Kohlrabi. Furthermore, the total glucosinolate and total isothiocyanate content of raw and fermented extracts of Kohlrabi were also evaluated.

The aim of this study was to investigate the antibacterial, antifungal, and anti-biofilm activities of the methanolic extract of marine Chlorella vulgaris along with its lipid composition, including carotenoids. The C. vulgaris strain, isolated from the Mediterranean coast of northern Morocco, was identified morphologically and molecularly by partial sequencing of the 18S rRNA gene. The methanolic extract of C. vulgaris demonstrated significant antibacterial activity against both Gram-positive and Gram-negative pathogenic bacteria, with MIC values ranging from 3.12 to 12.5 mg mL^-1. The antibiofilm activity of the extract was dose-dependent against Staphylococcus epidermidis and Pseudomonas aeruginosa. The extract demonstrated antifungal activity against four fungal strains. A. brasiliensis was shown to be the most sensitive species, whereas M. hiemalis was the least sensitive one. The identified lipid compounds mainly consisted of polar species e.g. phospholipids and carotenoids including lutein and neoxanthin, whose antimicrobial activity is generally recognised.

Anthocyanins, water-soluble flavonoids found in fruits and vegetables, exhibit diverse biological activities, with cyanidin being the most common pigment. While cyanidin's chemo preventive and -antioxidant activity is well-studied, its impact on inflammatory profile and migration capacity of leukemic cells are less understood. This study evaluates the effects of Cyanidin-3-glucoside (C3G) on the inflammatory mechanisms influencing leukemic cell migration. Results show that C3G doses up to 50 µM do not affect cell metabolism, viability, or cell cycle phases. C3G significantly reduces TNF-α, IL-8, CCL2 production, and the p-NFκB/NFκB ratio in LPS (Lipopolysaccharide)-challenged cells. It also diminishes migration rates in response to LPS or fMLP (N-formyl-methionyl-leucyl-phenylalanine) stimulation and reduces Rho-GTP expression. Thus, C3G modifies the inflammatory and migration properties of leukemic cells, highlighting the potential of anthocyanins as a complementary therapy and an avenue for further therapeutic intervention.

Rheum L. is an important genus of the family Polygonaceae comprised of nearly 60 herbaceous species distributed across the world. The members of Rheum genus are extensively used in curing several ailments, like wound healing, blood purification, menstruation problems, indigestion, headaches, chest pain, dysentery, stomachaches, fractured and broken bones, etc. The crude extracts and drugs derived from Rehum species possess anticancer, antimicrobial, anti-inflammatory, anti-SARS activity, and wound-healing properties. However, all the information on this genus is scattered and a systematic review of Rheum species is not available. The present study highlighted major distribution points, secondary metabolites, and pharmacological properties of Rheum species. A total of 144 unique compounds were recorded from Rheum genus, of them 24 were Stilbenes. This study further scientifically re-examines all published literature and explores market potential and pharmacology along with recommendations for the conservation of these high-value species.

Ent-Sauchinone is a bioactive lignan isolated from Saururus chinensis (Lour.) Baill could suppress the migration and invasion of hepatocellular carcinoma (HCC) cells. The study involves the structural modification of ent-sauchinone to augment its inhibitory effect on HCC progression and to investigate the mechanisms involved. In this study, a series of ent-sauchinone based amide derivatives were synthesised and the anticancer activities were evaluated against two hepatoma cell lines. Among them, compound 3 exhibited excellent resistance to migration and invasion by reversing the epithelial-mesenchymal transition (EMT) and inhibiting the phosphorylation of signal transducer and activator of transcription 3 (STAT3) and the expression of matrix metalloproteinases (MMPs). Molecular docking results indicate that compound 3 may exert its inhibitory effect on the progression of liver cancer by acting on the DNA-binding domain of STAT3. These results suggested that compound 3 could be a potential anticancer agent, especially for metastatic cancer therapy.