Morphologiai es igazsagugyi orvosi szemle最新文献

It is well established that the stroma of some tumours contains amyloid. Authors have studied in pleomorphic tumours the occurrence of amyloid. Out of 40 salivary gland tumours 5 contained amyloid in their stroma between tumour-cell cords. Amyloid of salivary gland tumours had a resistant structure. The possible pathomechanisms of amyloid deposition are discussed.

Authors have studied the changes in number and electronmicroscopic appearances of mast cells in the mucosa of large intestine of patients suffering from colitis ulcerosa either in its active or remission period. Counting of cells was performed in osmium-fixed, semithin, Giemsa-stained sections to avoid variations in staining due to formaldehyde. In the active stage of the disease the increase in number of mast cells was significant. Their pronounced degranulation was evident in both semi- and ultrathin preparations. Two forms of degranulation were distinguished: 1. by the release of granules; 2. by the extrusion of whole cytoplasmic areas. The latter indicated fast degranulation, in accordance with observations in animal experiments. Mast cells became closely related to the capillaries, Schwann-cells and nerve fibres of the lamina propria, with myofibroblasts and collagenous fibres. At some places they were found between the epithelial cells of Lieberkühn's crypts. It is assumed that this close topographical correlation represents also functional connections. The present morphological findings corroborate current hypotheses and suggest that the increase in number of mast cells in the active stage of coliitis ulcerosa mediates an hyperergic reaction.

The peanut-agglutinin binding of lymph node follicles was studied in frozen and paraffin sections of human tonsils. In a substantial fraction (10-25%) of follicles focal zones of high PNA receptor expression were encountered which were more readily recognizable in paraffin sections and corresponded to the centrocytous, "light" zone of the follicle. Results suggest that divergent lectin binding of follicles reflects their different functional states.

Interferences were made with a high-energy carbondioxide laser on the tongue and buccal mucosa of albino rats. Tissue destructions -- and cryodestruction reported in the literature -- were comparatively evaluated. At the edge of the laser cut a 30 micron carbonization zone occurred, beyond this a 150 micron necrotic zone and an 800 micron transition zone were observed. These were irreversibly damaged territories with coagulated blood vessels. The surrounding was hyperaemic and edematous. The application of higher energy brings about less tissue damage with narrower zones. A similar but more widely damaged destruction occurs after electrocauterization. Here, irreversibly damaged areas are 5 to 8-times wider than those found with laser. The largest damaged area was found after cryodestruction. It is thus concluded that the use of a high-energy carbondioxide laser results in the smallest irreversibly damaged tissue area. This explains the clinical advantages of the laser knife: coagulation during cutting, reduced postoperative pain and edema, small scar and good functional and aesthetic restitution.

Morphometric and histochemical changes of muscle spindles and tendon spindles of Golgi were studied in the soleus and gastrocnemius muscles of tenotomized and immobilized rats. The number of intrafusal fibres and mechanoreceptors did not change after either tenotomy or immobilization. The diameter of intrafusal fibres decreased by 14 to 40% three weeks after tenotomy or immobilization. The histochemical characteristics of muscle fibres were also changed. The capsule of mechanoreceptors was found thickened, the intracapsular space disappeared. The capsule was tensely attached to the mechanoreceptors.

Repeated subcutaneous injections of cholecystokinin-octapeptide has brought about an acute interstitial pancreatitis in rats. The aim of the experiment was the study of histological and biochemical alterations with special regard to regeneration phenomena. The treatment was carried out for 1 (group 1), 3,5 and 7 (group 2) days. Morphological alterations were more pronounced in group 2 than in group 1, but the pathological process was principally similar in both groups. Lowest values of pancreatic weight, protein and DNA content were attained in both groups at day 5. After this, protein and DNA started to increase. Hyperstimulation with CCK-OP on day 5 did not increase the degree of pancreas damage, nor did it prevent morphological and functional regeneration. The reason of this may be the decreased CCK-OP sensitivity of pancreatic acinar cells and/or the increased CCK-OP tolerance of newly formed acinar cells.

Authors have studied in apostematous pyelonephritis induced by the ligation of the ureter and the intravenous injection of E coli bacteria the localization and elimination with time of the pathogen. The pathogen was demonstrated by light and electron microscopy, its parietel antigen was localized with the light microscopic peroxidase antiperoxidase and post-embedding electron microscopic immunogold techniques. Two days after inoculation the suppurative inflammation of tubulo-interstitial foci was observed; in the capillaries, interstitium, and tubuli, free and phagocyted bacteria were encountered. In the interstitium, in the proximal tubuli and in the capillary space of some glomeruli bacterial groups were observed. Intracapillary bacteria were attached by their outer wall to the surface of endothelial cells. In the tubuli this adherence occurred with pili or with the outer layer of bacterial wall. From the seventh day after inoculation macrophages containing PAS-positive globuli appeared in the interstitium. Under the electron microscope these globuli proved to be features composed of myelin figures of phagolysosomal origin. Globuli and the myelin figures possessed an E. coli antigenicity. Thirteen weeks after inoculation E. coli antigen positivity was found in the cytoplasm of inflammatory cells in the tubular walls and in the suppurative cylinders, The organism was apparently unable to eliminate the materials derived from the pathogenic microorganisms.

Authors report the serologically verified CMV hepatitis of a 35-year old man whose hepatic alteration appeared in the form of a granulomatous hepatitis. On this account the characteristics of granulomatous hepatitis cases published so far in the literature are surveyed.

By the immunohistochemical demonstration of SR calcium ATPase and myoglobin a fibre classification method was developed. Fast fibres showed intense, while slow fibres weak SR calcium ATPase reactivity. Immunohistochemical reaction of myoglobin characterized the oxidative metabolic state of fibres similar to the succinate dehydrogenase (SDH) reaction. By means of SR calcium ATPase and myoglobin immunohistochemistry fibres were classified as slow oxidative (SO), fast oxidative glycolytic (FOG) and fast glycolytic (Fg) groups. The SR calcium ATPase activity of the different fibres varied in the FG greater than FOG greater than SO order, while myoglobin immunoreactivity in the FOG greater than SO greater than FG order. Both proteins studied preserved their antigenicities in Bouin's fixative or in formol-acetate and paraffin embedding. The light microscopic immunogold-silver method was found suitable also for electron microscopy. The silver intensification of small particle-size (5 nm) gold conjugate results in a reaction with the joint advantages of high sensitivity and optimal visibility. The described immunohistochemical method proved to be suitable for the retrospective differentiation of human biopsy materials.