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Molecular and technical aspects on the interaction of bovine serum albumin with pyrazine derivatives: From molecular docking to spectroscopy study
IF 3.2 2区 农林科学 Q2 FOOD SCIENCE & TECHNOLOGY Pub Date : 2025-02-03 DOI: 10.1111/1750-3841.70017
Wenghua Tong, XingYan Liu, Ying Yang, Yan Wang, Zhijiu Huang, Hongjun Fan

In order to better understand the transport and action mechanism of flavor substance and proteins in human body, the interaction mechanism between pyrazine derivatives and bovine serum albumin (BSA) was studied by molecular dynamics simulation and a series of spectroscopic methods. In molecular docking, it was observed that the small molecules were surrounded by hydrophobic amino acid residues of the protein, and the main amino acid residues formed π–π interaction and hydrogen bond interaction with BSA. The results of fluorescence emission spectroscopy combined with thermodynamic analysis showed that static quenching was the main mechanism of the interaction between three pyrazine derivatives and BSA, which was dominated by hydrophobic interaction. Synchronous fluorescence spectroscopy and three-dimensional fluorescence spectroscopy combined with molecular dynamics simulation proved that the pyrazine derivatives changed the conformation of BSA. In summary, pyrazine derivatives can interact with BSA, and the complexation of the complex changes its spatial conformation. The research in this paper has positive significance for understanding the binding, transport, and metabolism of pyrazine compounds in the process of blood circulation and provides key data for the metabolism of pyrazine compounds in vivo.

Practical Application

  • The interaction of pyrazine derivatives-BSA is studied by multi-spectra and MD.
  • The fluorescence quenching of pyrazine derivatives-BSA is static quenching.
  • The main force between pyrazine derivatives and BSA is hydrophobic force.
  • There is only one site of association between pyrazine derivatives and BSA.
  • Pyrazine derivatives have effects on conformation of BSA.
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引用次数: 0
Dynamic changes of drying behavior, physicochemical quality, and volatile oil of Exocarpium citri grandis under different drying temperatures
IF 3.2 2区 农林科学 Q2 FOOD SCIENCE & TECHNOLOGY Pub Date : 2025-02-03 DOI: 10.1111/1750-3841.17654
Wenling Sun, Yanhong Liu, Dengwen Lei, Lixuan Wei, Jiale Guo

Drying is the primary processing step in Exocarpium citri grandis (ECG), yet its quality changes throughout the drying process have not been elucidated. Dynamic changes of drying behavior, structure, color, active components, antioxidant properties, and volatile oil in ECG under different drying temperatures (50, 60, 70, and 80°C) were investigated, and the quality change mechanism was revealed. Results showed that the moisture ratio decreased rapidly and that structural damage occurred as moisture content decreased from 1.0000 to 0.2175 g/g (on a dry basis, d.b.), which accelerated flavonoids, polysaccharides, volatile oil, and antioxidant activity degradation, whereas levels of naringin and rhoifolin were increased. The above quality change process was accelerated with the increasing temperature. Correlation analysis revealed that active components had a synergistic effect on antioxidant activity, and the total color difference (ΔE) value and that active components could be well fitted by a linear mathematical model with correlation coefficient (R2) of 0.7749–0.9781. Moreover, all the indicators were uniformly quantified using comprehensive evaluation method to obtain a comprehensive score, which was the highest (0.57) at 70°C, with high drying efficiency, well quality, and low energy consumption. The biosynthesis pathway verified that the species of volatile oil components increased, including newly terpenes, alcohols, aldehydes, esters, and others after 0.2175 g/g. This study revealed that the total content of active components in ECG decreased before 0.2175 g/g, and then the metabolite content and species increased during drying, as well as obtained the optimal drying temperature (70°C).

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引用次数: 0
Oral administration of Lactobacillus rhamnosus MP108 ameliorates hemolytic jaundice in rats
IF 3.2 2区 农林科学 Q2 FOOD SCIENCE & TECHNOLOGY Pub Date : 2025-02-03 DOI: 10.1111/1750-3841.17671
Gaohong Wu, Qing Ai, Yuan Shi

Accumulating evidence suggests that specific probiotic strains exert effects on hemolytic jaundice (HJ), and probiotic strains of Lactobacillus rhamnosus exhibit potential beneficial effects against HJ. This study aimed to determine the effects of L. rhamnosus MP108 (MP108) on rats with acetylphenylhydrazine (APH)-induced HJ. One week of oral MP108 administration (16 × 109 CFU/kg·day) significantly reversed the HJ-induced body-weight reduction and normalized serum levels of serum alanine aminotransferase (ALT), aspartate aminotransferase (AST), total bilirubin (TBIL), total superoxide dismutase (T-SOD), glutathione peroxidase (GSH-PX), hydrogen peroxide (H2O2), and superoxide anion (O2−) in HJ rats. Furthermore, significant improvements were observed in the pathological changes of liver and intestinal tissues. 16S rRNA high-throughput sequencing of fecal samples demonstrated that MP108 altered gut microbiota composition by increasing Lacticaseibacillus spp. abundance, which correlated with the serum levels of ALT, AST, TBIL, T-SOD, GSH-PX, H2O2, and O2−. In summary, these results provide evidence that MP108 has the potential to improve HJ symptoms by alleviating hepatic impairment, which is associated with changes in gut microbiota composition.

Practical Application

The study indicates that MP108 can modulate the gut microbiota, improve liver function, and thereby alleviate the symptoms of hemolytic jaundice (HJ). These findings suggest a promising therapeutic approach for HJ, offering potential benefits to patients with related conditions.

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引用次数: 0
Smart packaging films based on gelatin/κ-carrageenan integrated with gromwell root extract rich in shikonin and carbon dots for real-time monitoring of shrimp freshness
IF 3.2 2区 农林科学 Q2 FOOD SCIENCE & TECHNOLOGY Pub Date : 2025-02-03 DOI: 10.1111/1750-3841.70011
Gokulprasanth Murugan, Ajahar Khan, Ruchir Priyadarshi, Krisana Nilsuwan, Soottawat Benjakul, Jong-Whan Rhim

Multifunctional pH-responsive gelatin/κ-carrageenan (GC) blend films containing gromwell (Lithospermum erythrorhizon [LE]) root ethanolic extract (LE-EE) rich in shikonin or shikonin rich extract (SRE) and carbon dots (LE carbon dots [LE-CDs]) were prepared and characterized. The hydrothermal method was adopted for the synthesis of LE-CDs, which displayed a blue color under UV light. The obtained LE-CDs possessed exceptional UV barrier, antioxidant and antimicrobial activities. The enhanced activities were recorded when the level of LE-CDs upsurged (p < 0.05). Transmission electron microscopic (TEM) and Fourier transform infrared (FTIR) results revealed the typical morphology and chemical composition of LE-CDs. LE-CDs of 1 and 3% (w/w) were incorporated as the active fillers along with SRE into the GC blend film by the solvent casting method. Developed films showed a slight decrease in tensile and water vapor barrier properties with the inclusion of both additives. Color and opaqueness of the film became darker as the additives were incorporated, whereas the thermal property was greatly enhanced. Film containing 3% LE-CDs blocked UV-A and UV-B by 93.30 and 99.81%, respectively. GC/SRE/3%CD film exhibited strong radical scavenging and antibacterial activities against Listeria monocytogenes and Escherichia coli, in which the growth was terminated after 12 h. Film had a pH-dependent color change, depending on various pH levels (2–12). Shrimp freshness could be monitored as indicated by the shift to bluish color after 48 h. Therefore, this finding indicated that incorporating biomass-derived CDs and natural colorants into biopolymer films, especially GC blend films, could offer diverse strategies for maintaining safety and prolonging shelf life in response to the growing need for smart packaging for food applications.

{"title":"Smart packaging films based on gelatin/κ-carrageenan integrated with gromwell root extract rich in shikonin and carbon dots for real-time monitoring of shrimp freshness","authors":"Gokulprasanth Murugan,&nbsp;Ajahar Khan,&nbsp;Ruchir Priyadarshi,&nbsp;Krisana Nilsuwan,&nbsp;Soottawat Benjakul,&nbsp;Jong-Whan Rhim","doi":"10.1111/1750-3841.70011","DOIUrl":"10.1111/1750-3841.70011","url":null,"abstract":"<p>Multifunctional pH-responsive gelatin/κ-carrageenan (GC) blend films containing gromwell (<i>Lithospermum erythrorhizon</i> [LE]) root ethanolic extract (LE-EE) rich in shikonin or shikonin rich extract (SRE) and carbon dots (LE carbon dots [LE-CDs]) were prepared and characterized. The hydrothermal method was adopted for the synthesis of LE-CDs, which displayed a blue color under UV light. The obtained LE-CDs possessed exceptional UV barrier, antioxidant and antimicrobial activities. The enhanced activities were recorded when the level of LE-CDs upsurged (<i>p</i> &lt; 0.05). Transmission electron microscopic (TEM) and Fourier transform infrared (FTIR) results revealed the typical morphology and chemical composition of LE-CDs. LE-CDs of 1 and 3% (<i>w/w</i>) were incorporated as the active fillers along with SRE into the GC blend film by the solvent casting method. Developed films showed a slight decrease in tensile and water vapor barrier properties with the inclusion of both additives. Color and opaqueness of the film became darker as the additives were incorporated, whereas the thermal property was greatly enhanced. Film containing 3% LE-CDs blocked UV-A and UV-B by 93.30 and 99.81%, respectively. GC/SRE/<sup>3%</sup>CD film exhibited strong radical scavenging and antibacterial activities against <i>Listeria monocytogenes</i> and <i>Escherichia coli</i>, in which the growth was terminated after 12 h. Film had a pH-dependent color change, depending on various pH levels (2–12). Shrimp freshness could be monitored as indicated by the shift to bluish color after 48 h. Therefore, this finding indicated that incorporating biomass-derived CDs and natural colorants into biopolymer films, especially GC blend films, could offer diverse strategies for maintaining safety and prolonging shelf life in response to the growing need for smart packaging for food applications.</p>","PeriodicalId":193,"journal":{"name":"Journal of Food Science","volume":"90 2","pages":""},"PeriodicalIF":3.2,"publicationDate":"2025-02-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143078028","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Sustainable extraction of carotenoids from macauba pulp and press-cake: Use of ethyl acetate and ultrasound as optimization strategies
IF 3.2 2区 农林科学 Q2 FOOD SCIENCE & TECHNOLOGY Pub Date : 2025-02-03 DOI: 10.1111/1750-3841.70030
Danúbia Joanes Rosa Guerra, Caroline Wolffel Silva, Amanda Lais Alves Almeida Nascimento, Paulo César Stringheta, Moysés Naves de Moraes, Ceres Mattos Della Lucia, Frederico Augusto Ribeiro de de Barros

Macauba (Acrocomia aculeata) is a Brazilian palm whose pulp is rich in carotenoids and holds potential for the development of healthy foods. This study presents an innovative approach utilizing ethyl acetate, a solvent recognized as generally safe (GRAS), in conjunction with ultrasound technology, to optimize the extraction of carotenoids from both macauba pulp (MP) and its pulp press-cake (PPC). The effects of varying time and temperature parameters (5–30 min at 25–60°C) alongside ultrasound conditions (25 and 60°C at frequencies of 25 and 45 kHz) on the extraction process were evaluated, with total carotenoid content quantified via spectrophotometry. For MP, the optimal extraction conditions were 60°C for 30 min, yielding 219.33 µg/g of carotenoids, while ultrasound at 45 kHz, under the same time/temperature, enhanced the extraction efficiency to 277.55 µg/g. In the case of PPC, ultrasound extraction achieved a carotenoid content of 124.23 µg/g. Overall, the findings indicate that elevating the temperature to 60°C favored the extraction process, while ultrasound demonstrated effective even under milder conditions. This study emphasizes the potential of ethyl acetate as a sustainable alternative to traditional toxic solvents, aligning with the growing demand for safer and more environmentally friendly practices in the extraction of bioactive compounds.

Practical Application

The sustainable extraction of carotenoids from macauba, a non-conventional source, shows great potential for producing natural colorants and antioxidants in the food industry. The efficient use of ultrasound in the extraction process highlights the potential of this technology for the industry, providing an eco-friendly and effective alternative to conventional methods.

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引用次数: 0
Comparison of major carotenoid composition in palm fruits (Arecaceae): An analysis of oil palm fruits and the colored exocarp fruits of four palm species
IF 3.2 2区 农林科学 Q2 FOOD SCIENCE & TECHNOLOGY Pub Date : 2025-02-03 DOI: 10.1111/1750-3841.70026
Heriyanto, Jonatan Effendi, Yuyun Yuniati, Yuzo Shioi

The oil palm, a widely studied species of palm, is a crucial source of edible oil and pro-vitamin A carotenoids, primarily α- and β-carotene. The diverse peel and pulp colors (yellow–orange–red) of various palm fruits suggest the presence of not only pro-vitamin A carotenoids but also other bioactive carotenoids like lutein and lycopene, which offer additional health benefits. This study aimed to identify and quantify the major carotenoids in fruits with colored exocarps of four varieties of palm species belonging to different genera and compare them to those in oil palm fruit to evaluate their bioactive potential for human health. High-performance liquid chromatography and mass spectrometry were used for chromatographic and spectrometric analyses using non-saponified samples, except for lutein estimation, revealing four major carotenoids: α- and β-carotene, lycopene, and lutein, which together accounted for more than 50% of the total carotenoid content. Among the five palm fruits tested (MacArthur, Manila, mountain date, foxtail, and oil palms), foxtail palm exhibited the highest concentration of major carotenoids at 500 µg/g dry weight (d.w.), with β-carotene comprising 69% of this total, followed by MacArthur palm (235 µg/g d.w.), whereas mountain date palm had the lowest concentration of major carotenoids at 135 µg/g d.w. These findings suggest that colored palm fruits are significant sources of both pro-vitamin A carotenoids and other beneficial carotenoids like lycopene and lutein. The method of sample preparation, particularly the inclusion or omission of a saponification process, plays a critical role in the recovery and accurate quantification of carotenoid concentrations due to their varying susceptibility to this process.

{"title":"Comparison of major carotenoid composition in palm fruits (Arecaceae): An analysis of oil palm fruits and the colored exocarp fruits of four palm species","authors":"Heriyanto,&nbsp;Jonatan Effendi,&nbsp;Yuyun Yuniati,&nbsp;Yuzo Shioi","doi":"10.1111/1750-3841.70026","DOIUrl":"10.1111/1750-3841.70026","url":null,"abstract":"<p>The oil palm, a widely studied species of palm, is a crucial source of edible oil and pro-vitamin A carotenoids, primarily α- and β-carotene. The diverse peel and pulp colors (yellow–orange–red) of various palm fruits suggest the presence of not only pro-vitamin A carotenoids but also other bioactive carotenoids like lutein and lycopene, which offer additional health benefits. This study aimed to identify and quantify the major carotenoids in fruits with colored exocarps of four varieties of palm species belonging to different genera and compare them to those in oil palm fruit to evaluate their bioactive potential for human health. High-performance liquid chromatography and mass spectrometry were used for chromatographic and spectrometric analyses using non-saponified samples, except for lutein estimation, revealing four major carotenoids: α- and β-carotene, lycopene, and lutein, which together accounted for more than 50% of the total carotenoid content. Among the five palm fruits tested (MacArthur, Manila, mountain date, foxtail, and oil palms), foxtail palm exhibited the highest concentration of major carotenoids at 500 µg/g dry weight (d.w.), with β-carotene comprising 69% of this total, followed by MacArthur palm (235 µg/g d.w.), whereas mountain date palm had the lowest concentration of major carotenoids at 135 µg/g d.w. These findings suggest that colored palm fruits are significant sources of both pro-vitamin A carotenoids and other beneficial carotenoids like lycopene and lutein. The method of sample preparation, particularly the inclusion or omission of a saponification process, plays a critical role in the recovery and accurate quantification of carotenoid concentrations due to their varying susceptibility to this process.</p>","PeriodicalId":193,"journal":{"name":"Journal of Food Science","volume":"90 2","pages":""},"PeriodicalIF":3.2,"publicationDate":"2025-02-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143078264","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Conjugated linoleic acid in cheese: A review of the factors affecting its presence
IF 3.2 2区 农林科学 Q2 FOOD SCIENCE & TECHNOLOGY Pub Date : 2025-02-03 DOI: 10.1111/1750-3841.70021
Maria Govari, Patroklos Vareltzis

Several health benefits of conjugated linoleic acid (CLA) have been documented. The present work is aimed to review data on the various factors affecting the CLA content in cheese of studies accomplished in the last decade and also indicating the factors that increase the CLA levels. The CLA content in cheese depends on CLA levels present in milk, since the lipids with the CLA are transferred from milk into the cheese. Feed types rich in α-linolenic and linoleic acids such as pasture grass, plant oils, cereals rich in oil, or fish oils can affect the CLA level in milk. In contrast to findings of previous reviews made in previous decade, which stated that the CLA levels in cheese were stable during ripening time, the present review reveals that certain lactic acid bacteria, that is, probiotic Lactiplantibacillus plantarum, Lactobacillus acidophilus, or Lacticaseibacillus casei, as well as Bifidobacterium lactis can increase the CLA levels in cheese by converting linoleic acid during ripening time. These bacteria starters increased the CLA levels by 1.19, 1.6, and 6.6 times as much as the control in Ovine model, Miniature, and Cheddar cheese, respectively. Lipid oxidation due to factors like fluorescent light or aerobic conditions can decrease the CLA levels during storage.

{"title":"Conjugated linoleic acid in cheese: A review of the factors affecting its presence","authors":"Maria Govari,&nbsp;Patroklos Vareltzis","doi":"10.1111/1750-3841.70021","DOIUrl":"10.1111/1750-3841.70021","url":null,"abstract":"<p>Several health benefits of conjugated linoleic acid (CLA) have been documented. The present work is aimed to review data on the various factors affecting the CLA content in cheese of studies accomplished in the last decade and also indicating the factors that increase the CLA levels. The CLA content in cheese depends on CLA levels present in milk, since the lipids with the CLA are transferred from milk into the cheese. Feed types rich in <i>α</i>-linolenic and linoleic acids such as pasture grass, plant oils, cereals rich in oil, or fish oils can affect the CLA level in milk. In contrast to findings of previous reviews made in previous decade, which stated that the CLA levels in cheese were stable during ripening time, the present review reveals that certain lactic acid bacteria, that is, probiotic <i>Lactiplantibacillus plantarum</i>, <i>Lactobacillus acidophilus</i>, or <i>Lacticaseibacillus casei</i>, as well as <i>Bifidobacterium lactis</i> can increase the CLA levels in cheese by converting linoleic acid during ripening time. These bacteria starters increased the CLA levels by 1.19, 1.6, and 6.6 times as much as the control in Ovine model, Miniature, and Cheddar cheese, respectively. Lipid oxidation due to factors like fluorescent light or aerobic conditions can decrease the CLA levels during storage.</p>","PeriodicalId":193,"journal":{"name":"Journal of Food Science","volume":"90 2","pages":""},"PeriodicalIF":3.2,"publicationDate":"2025-02-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/1750-3841.70021","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143077843","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Evaluation of extruded quinoa flour on dough rheology and white salted noodles quality
IF 3.2 2区 农林科学 Q2 FOOD SCIENCE & TECHNOLOGY Pub Date : 2025-02-03 DOI: 10.1111/1750-3841.17646
Ruihan Huang, Kai Huang, Hongdong Song, Sen Li, Xiao Guan

White salted noodles are popular staples in Asia, but the ones with limited nutrient composition and high glycemic value still present significant concerns. This study investigated the potential of incorporating extruded quinoa flour (EQF) into wheat flour to enhance nutrient quality and reduce the starch digestibility of composite noodles. Moreover, the effect of EQF addition on the rheological properties of wheat dough and the cooking properties of composite noodles was also studied. The results showed that increasing the proportion of EQF in the composite flour led to a decrease in pasting viscosities and an increase in pasting temperatures, indicating that EQF inhibits starch gelatinization and retrogradation. The incorporation of EQF increased water absorption and softening degree while decreasing dough development time and viscoelasticity. The cooked noodles exhibited a significant reduction in water absorption, hardness, chewiness, and springiness, while an increase in cooking loss following EQF addition. Notably, noodles supplemented with EQF exhibited reduced overall starch digestibility with an increased digestion rate. Collectively, our results showed that substituting 10–20% EQF for wheat flour in noodles effectively lowered total starch digestibility and avoided high cooking loss. This study will have implications for the industrial application of quinoa as a staple food.

{"title":"Evaluation of extruded quinoa flour on dough rheology and white salted noodles quality","authors":"Ruihan Huang,&nbsp;Kai Huang,&nbsp;Hongdong Song,&nbsp;Sen Li,&nbsp;Xiao Guan","doi":"10.1111/1750-3841.17646","DOIUrl":"10.1111/1750-3841.17646","url":null,"abstract":"<p>White salted noodles are popular staples in Asia, but the ones with limited nutrient composition and high glycemic value still present significant concerns. This study investigated the potential of incorporating extruded quinoa flour (EQF) into wheat flour to enhance nutrient quality and reduce the starch digestibility of composite noodles. Moreover, the effect of EQF addition on the rheological properties of wheat dough and the cooking properties of composite noodles was also studied. The results showed that increasing the proportion of EQF in the composite flour led to a decrease in pasting viscosities and an increase in pasting temperatures, indicating that EQF inhibits starch gelatinization and retrogradation. The incorporation of EQF increased water absorption and softening degree while decreasing dough development time and viscoelasticity. The cooked noodles exhibited a significant reduction in water absorption, hardness, chewiness, and springiness, while an increase in cooking loss following EQF addition. Notably, noodles supplemented with EQF exhibited reduced overall starch digestibility with an increased digestion rate. Collectively, our results showed that substituting 10–20% EQF for wheat flour in noodles effectively lowered total starch digestibility and avoided high cooking loss. This study will have implications for the industrial application of quinoa as a staple food.</p>","PeriodicalId":193,"journal":{"name":"Journal of Food Science","volume":"90 2","pages":""},"PeriodicalIF":3.2,"publicationDate":"2025-02-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143077846","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
The impact of fruit size on internal browning in pineapples
IF 3.2 2区 农林科学 Q2 FOOD SCIENCE & TECHNOLOGY Pub Date : 2025-02-03 DOI: 10.1111/1750-3841.17622
Mengzhuo Zhang, Changsong Zhu, Qiting Na, Hui Cao, Cong Tian, Guangsen Liu, Lanhuan Meng

A typical symptom of post-harvest pineapple (Ananas comosus L.) internal browning is influenced by multiple factors. However, the precise mechanism through which fruit size influences the occurrence of browning remains unclear. Therefore, this study aimed to investigate the impacts of fruit size in browning and its possible mechanisms in harvested pineapple fruit using physical and biochemical analysis and RNA-seq. Disease incidence was assessed in four groups of fruits (1, 1.5, 2, and 2.5 ± 0.2 kg), with the two most significant groups (1 and 2.5 ± 0.2 kg) selected for detailed analysis. The results showed that the large pineapple fruits had faster browning senescence and membrane lipid peroxidation, higher respiration intensity, more reactive oxygen species accumulation, and higher malondialdehyde content. Likewise, lower antioxidant capacity such as ascorbic acid, ascorbate peroxidase, catalase, and superoxide dismutase and higher polyphenol oxidase activity, peroxidase activity, phenylalanine ammonia-lyase activity, and lipoxygenase activity. It can be concluded that the large pineapple fruits were severely subjected to oxygen stress and membrane lipid peroxidation during storage. Gene ontology enrichment reveals that this relates mainly to oxidoreductase and glutathione metabolism. The large pineapple fruits accelerated AsA–GSH cycle pathway metabolism. Real-time quantitative PCR shows downregulated expression of AcAPX1, AcAPX6, AcAPX6 × 1, AcCAT2, AcSOD[Fe]2, and AcSODX2 in large pineapple fruits, whereas AcPPO and AcLOX upregulated expression. This study offers insights into fruit size and browning. Future molecular techniques may reduce fruit browning.

{"title":"The impact of fruit size on internal browning in pineapples","authors":"Mengzhuo Zhang,&nbsp;Changsong Zhu,&nbsp;Qiting Na,&nbsp;Hui Cao,&nbsp;Cong Tian,&nbsp;Guangsen Liu,&nbsp;Lanhuan Meng","doi":"10.1111/1750-3841.17622","DOIUrl":"10.1111/1750-3841.17622","url":null,"abstract":"<p>A typical symptom of post-harvest pineapple (<i>Ananas comosus</i> L.) internal browning is influenced by multiple factors. However, the precise mechanism through which fruit size influences the occurrence of browning remains unclear. Therefore, this study aimed to investigate the impacts of fruit size in browning and its possible mechanisms in harvested pineapple fruit using physical and biochemical analysis and RNA-seq. Disease incidence was assessed in four groups of fruits (1, 1.5, 2, and 2.5 ± 0.2 kg), with the two most significant groups (1 and 2.5 ± 0.2 kg) selected for detailed analysis. The results showed that the large pineapple fruits had faster browning senescence and membrane lipid peroxidation, higher respiration intensity, more reactive oxygen species accumulation, and higher malondialdehyde content. Likewise, lower antioxidant capacity such as ascorbic acid, ascorbate peroxidase, catalase, and superoxide dismutase and higher polyphenol oxidase activity, peroxidase activity, phenylalanine ammonia-lyase activity, and lipoxygenase activity. It can be concluded that the large pineapple fruits were severely subjected to oxygen stress and membrane lipid peroxidation during storage. Gene ontology enrichment reveals that this relates mainly to oxidoreductase and glutathione metabolism. The large pineapple fruits accelerated AsA–GSH cycle pathway metabolism. Real-time quantitative PCR shows downregulated expression of <i>AcAPX1</i>, <i>AcAPX6</i>, <i>AcAPX6 ×</i> <i>1</i>, <i>AcCAT2</i>, <i>AcSOD[Fe]2</i>, and <i>AcSODX2</i> in large pineapple fruits, whereas <i>AcPPO</i> and <i>AcLOX</i> upregulated expression. This study offers insights into fruit size and browning. Future molecular techniques may reduce fruit browning.</p>","PeriodicalId":193,"journal":{"name":"Journal of Food Science","volume":"90 2","pages":""},"PeriodicalIF":3.2,"publicationDate":"2025-02-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143077989","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Physiochemical properties of hemp extract (Cannabis sativa L) inflorescences grown in Northern Alabama
IF 3.2 2区 农林科学 Q2 FOOD SCIENCE & TECHNOLOGY Pub Date : 2025-02-03 DOI: 10.1111/1750-3841.17642
Aaron Dudley (IFT Premier Member), Lamin Kassama, Armitra Jackson-Davis, Xianyan Kuang, Ernst Cebert

Medicinal plants are an excellent source of bioactive components and are gaining significant attention for food protection due to their bioactive properties. In this context, hemp (Cannabis sp.) is being explored for such applications because of its well-known antibacterial and antioxidant activities. However, the bioactive efficacy of cultivars currently grown in Northern Alabama has not been widely studied. Therefore, the purpose of this study was to evaluate the physicochemical properties of two hemp cultivars (Hemp 5 and Hemp 17). Hemp inflorescences grown at the Winfred Thomas Agricultural Research Station were used in this study. The antioxidant activity and content of the extracts were determined by ferric reducing antioxidant power, radical scavenging activity, total phenolic content, and total flavonoid content. Antibacterial activity against cocktails of Listeria monocytogenes (LM) and Salmonella enterica (SE) was evaluated by optical density and disc diffusion. All treatments were analyzed in triplicate, and analysis of variance was conducted with statistical significance based on p ≤ 0.05. Results indicated that cultivars and defatting significantly affected (p ≤ 0.05) the antioxidant properties, with Hemp 17 DF (defatted) having the highest RSA (70.51 ± 4.24%) compared to the positive control of ascorbic acid (83.81 ± 5.85%). Antibacterial results based on optical density indicated that hemp extracts had a significantly (p ≤ 0.05) lower optical density compared to the negative controls (LM and SE). These findings suggest that Northern Alabama hemp cultivars can potentially be utilized to enhance food safety and quality.

Practical Application

Northern Alabama cultivars of hemp extract can be utilized for the enhancement of the safety and quality application due to inherent antioxidant and antimicrobial properties.

{"title":"Physiochemical properties of hemp extract (Cannabis sativa L) inflorescences grown in Northern Alabama","authors":"Aaron Dudley (IFT Premier Member),&nbsp;Lamin Kassama,&nbsp;Armitra Jackson-Davis,&nbsp;Xianyan Kuang,&nbsp;Ernst Cebert","doi":"10.1111/1750-3841.17642","DOIUrl":"10.1111/1750-3841.17642","url":null,"abstract":"<div>\u0000 \u0000 <section>\u0000 \u0000 \u0000 <p>Medicinal plants are an excellent source of bioactive components and are gaining significant attention for food protection due to their bioactive properties. In this context, hemp (<i>Cannabis</i> sp.) is being explored for such applications because of its well-known antibacterial and antioxidant activities. However, the bioactive efficacy of cultivars currently grown in Northern Alabama has not been widely studied. Therefore, the purpose of this study was to evaluate the physicochemical properties of two hemp cultivars (Hemp 5 and Hemp 17). Hemp inflorescences grown at the Winfred Thomas Agricultural Research Station were used in this study. The antioxidant activity and content of the extracts were determined by ferric reducing antioxidant power, radical scavenging activity, total phenolic content, and total flavonoid content. Antibacterial activity against cocktails of <i>Listeria monocytogenes</i> (LM) and <i>Salmonella enterica</i> (SE) was evaluated by optical density and disc diffusion. All treatments were analyzed in triplicate, and analysis of variance was conducted with statistical significance based on <i>p</i> ≤ 0.05. Results indicated that cultivars and defatting significantly affected (<i>p</i> ≤ 0.05) the antioxidant properties, with Hemp 17 DF (defatted) having the highest RSA (70.51 ± 4.24%) compared to the positive control of ascorbic acid (83.81 ± 5.85%). Antibacterial results based on optical density indicated that hemp extracts had a significantly (<i>p</i> ≤ 0.05) lower optical density compared to the negative controls (LM and SE). These findings suggest that Northern Alabama hemp cultivars can potentially be utilized to enhance food safety and quality.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Practical Application</h3>\u0000 \u0000 <p>Northern Alabama cultivars of hemp extract can be utilized for the enhancement of the safety and quality application due to inherent antioxidant and antimicrobial properties.</p>\u0000 </section>\u0000 </div>","PeriodicalId":193,"journal":{"name":"Journal of Food Science","volume":"90 2","pages":""},"PeriodicalIF":3.2,"publicationDate":"2025-02-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143078012","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
期刊
Journal of Food Science
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