Pub Date : 2021-09-16DOI: 10.13057/nusbiosci/n130204
Asni Johari, M. Hermanto, Tia Wulandari
Abstract. Johari A, Hermanto MA, Wulandari T. 2021. Ant diversity inhabited oil palm plantations in a peatland in Sumatra, Indonesia. Nusantara Bioscience 13: 158-163. Ant community is one of the soil biotic components with diverse roles, including pollinators, predators, decomposers, parasites, and herbivores. Changes in land use from natural habitats to plantations affect the diversity and composition of ants because these groups are very vulnerable to environmental changes. The purpose of the study was to analyze the diversity of ants (Formicidae) found in peatland planted with oil palm. The study was conducted on peatland in Gambut Jaya village, Sungai Gelam sub-district, Muaro Jambi district, Indonesia from October 2019 to May 2020. The research was conducted through surveys in two peatland locations (natural peatland and oil palm plantation) using the purposive sampling technique through the stages of transect making, field sampling, sample preparation, and identification. A total of 2636 individuals of ants were collected from natural peatland, while 3183 individuals were found in oil palm plantations. Eight ant species were identified from both study sites belong to four subfamilies, namely Dolichoderinae, Formicinae, Myrmicinae, and Ponerinae. Among the species, Dolichoderus thoracicus were most dominant, composed 63.6% in natural peat-land and 73.6% in oil palm plantation. The diversity index of ant species in natural peatland was higher (0.97) than that in oil palm plantations (0.88), indicate the change in the abundance and diversity of ant species in oil palm plantations. Therefore, efforts to preserve the habitat of ants by maintaining their microhabitat and resource, as well as conserving the important species such as natural enemies of pests, need to be taken into account in peatland management.
{"title":"Ant diversity inhabited oil palm plantations in a peatland in Sumatra, Indonesia","authors":"Asni Johari, M. Hermanto, Tia Wulandari","doi":"10.13057/nusbiosci/n130204","DOIUrl":"https://doi.org/10.13057/nusbiosci/n130204","url":null,"abstract":"Abstract. Johari A, Hermanto MA, Wulandari T. 2021. Ant diversity inhabited oil palm plantations in a peatland in Sumatra, Indonesia. Nusantara Bioscience 13: 158-163. Ant community is one of the soil biotic components with diverse roles, including pollinators, predators, decomposers, parasites, and herbivores. Changes in land use from natural habitats to plantations affect the diversity and composition of ants because these groups are very vulnerable to environmental changes. The purpose of the study was to analyze the diversity of ants (Formicidae) found in peatland planted with oil palm. The study was conducted on peatland in Gambut Jaya village, Sungai Gelam sub-district, Muaro Jambi district, Indonesia from October 2019 to May 2020. The research was conducted through surveys in two peatland locations (natural peatland and oil palm plantation) using the purposive sampling technique through the stages of transect making, field sampling, sample preparation, and identification. A total of 2636 individuals of ants were collected from natural peatland, while 3183 individuals were found in oil palm plantations. Eight ant species were identified from both study sites belong to four subfamilies, namely Dolichoderinae, Formicinae, Myrmicinae, and Ponerinae. Among the species, Dolichoderus thoracicus were most dominant, composed 63.6% in natural peat-land and 73.6% in oil palm plantation. The diversity index of ant species in natural peatland was higher (0.97) than that in oil palm plantations (0.88), indicate the change in the abundance and diversity of ant species in oil palm plantations. Therefore, efforts to preserve the habitat of ants by maintaining their microhabitat and resource, as well as conserving the important species such as natural enemies of pests, need to be taken into account in peatland management.","PeriodicalId":19481,"journal":{"name":"Nusantara Bioscience","volume":" ","pages":""},"PeriodicalIF":0.4,"publicationDate":"2021-09-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"43339897","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2021-09-16DOI: 10.13057/nusbiosci/n130205
Cindy Marina Dasrat, Gyanpriya Maharaj
Abstract. Dasrat CM, Maharaj G. 2021. Biological control of mosquitoes with odonates: A case study in Guyana. Nusantara Bioscience 13: 163-170. Mosquitoes have plagued the lives of tropical residents as pests. However, due to their role as vectors of life-threatening diseases, controlling their population is necessary, especially in areas of prevalence – the Caribbean and Equatorial regions. In Guyana, we employ chemical treatments sub-regionally to eradicate mosquitoes. However, this treatment has limited success and is harmful to the environment. Therefore, our study focused on an environmentally friendly method such as biological control. This study was conducted at the University of Guyana, Turkeyen campus, where we exploited Odonata; Anisoptera, and Zygoptera as natural predators of mosquitoes of the genus Culex. The feeding efficiency and behaviors of naiads from the families of Libellulidae and Coenagrionidae were assessed during April – May of 2018. Each naiad was fed three Culine pupae and larvae, then observed for 60 minutes. We found that both groups pursue soft-bodied larvae and Libellulidae naiads are more efficient predators due to the higher feeding rate than Coenagrionidae. This finding is related to a behavioral study where we observed Libellulidae as active hunters that masticate and consume faster than Coenagrionidae, which are lay and wait, opportunistic predators that swallow their prey. We concluded that Libellulidae is an efficient predator for mosquito larvae of the Culex genus, and we found that there is a link between morphologic characteristics and feeding behaviors. We hope to use these results as a baseline study to develop mosquito biological control in Guyana. These can be used to reduce mosquito populations and the occurrence of vector diseases as well as improve integrated pest and vector management.
{"title":"Biological control of mosquitoes with odonates: A case study in Guyana","authors":"Cindy Marina Dasrat, Gyanpriya Maharaj","doi":"10.13057/nusbiosci/n130205","DOIUrl":"https://doi.org/10.13057/nusbiosci/n130205","url":null,"abstract":"Abstract. Dasrat CM, Maharaj G. 2021. Biological control of mosquitoes with odonates: A case study in Guyana. Nusantara Bioscience 13: 163-170. Mosquitoes have plagued the lives of tropical residents as pests. However, due to their role as vectors of life-threatening diseases, controlling their population is necessary, especially in areas of prevalence – the Caribbean and Equatorial regions. In Guyana, we employ chemical treatments sub-regionally to eradicate mosquitoes. However, this treatment has limited success and is harmful to the environment. Therefore, our study focused on an environmentally friendly method such as biological control. This study was conducted at the University of Guyana, Turkeyen campus, where we exploited Odonata; Anisoptera, and Zygoptera as natural predators of mosquitoes of the genus Culex. The feeding efficiency and behaviors of naiads from the families of Libellulidae and Coenagrionidae were assessed during April – May of 2018. Each naiad was fed three Culine pupae and larvae, then observed for 60 minutes. We found that both groups pursue soft-bodied larvae and Libellulidae naiads are more efficient predators due to the higher feeding rate than Coenagrionidae. This finding is related to a behavioral study where we observed Libellulidae as active hunters that masticate and consume faster than Coenagrionidae, which are lay and wait, opportunistic predators that swallow their prey. We concluded that Libellulidae is an efficient predator for mosquito larvae of the Culex genus, and we found that there is a link between morphologic characteristics and feeding behaviors. We hope to use these results as a baseline study to develop mosquito biological control in Guyana. These can be used to reduce mosquito populations and the occurrence of vector diseases as well as improve integrated pest and vector management.","PeriodicalId":19481,"journal":{"name":"Nusantara Bioscience","volume":" ","pages":""},"PeriodicalIF":0.4,"publicationDate":"2021-09-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"44542821","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Abstract. Afrianto WF, Wati SI, Hidayatullah T. 2021. The suitability assessment of the tree species in the urban parks and urban forest in Kediri City, East Java, Indonesia. Nusantara Bioscience 13: 131-139. Kediri has several urban parks and an urban forest as green open space (GOS) which have essential roles in economical, social, aesthetical, and ecological aspects. Generally, urban trees provide ecosystem services, such as carbon sequestration, stormwater attenuation, energy conservation, habitat for wildlife, air, water, and noise pollutants reduction. The present study aimed to (i) determine tree diversity in five urban parks and an urban forest of Kediri City and (ii) evaluate the suitability of tree species based on three indicators (silviculture, management, and aesthetic). This study was conducted in five urban parks and one urban forest in Kediri City. The suitability assessment was carried out through a literature review. The results showed that 29 tree species belonging to 17 families were found in the study sites, with the family Fabaceae being the most dominant one with five species. The number of exotic tree species (17) was higher than that of the native ones (12). The suitability assessment results showed that 21 tree species had a high score of 39-44, and only eight urban tree species had a medium score of 36-38. The urban parks and urban forests in Kediri were managed for the conservation of germplasm, recreation, and protection. The shapes were clumped and line. This study will contribute information to be used as guidance for urban tree management and policy framework.
{"title":"The suitability assessment of the tree species in the urban parks and urban forest in Kediri City, East Java, Indonesia","authors":"Whisnu Febry Afrianto, Susanti Indriya Wati, Taufiqi Hidayatullah","doi":"10.13057/nusbiosci/n130201","DOIUrl":"https://doi.org/10.13057/nusbiosci/n130201","url":null,"abstract":"Abstract. Afrianto WF, Wati SI, Hidayatullah T. 2021. The suitability assessment of the tree species in the urban parks and urban forest in Kediri City, East Java, Indonesia. Nusantara Bioscience 13: 131-139. Kediri has several urban parks and an urban forest as green open space (GOS) which have essential roles in economical, social, aesthetical, and ecological aspects. Generally, urban trees provide ecosystem services, such as carbon sequestration, stormwater attenuation, energy conservation, habitat for wildlife, air, water, and noise pollutants reduction. The present study aimed to (i) determine tree diversity in five urban parks and an urban forest of Kediri City and (ii) evaluate the suitability of tree species based on three indicators (silviculture, management, and aesthetic). This study was conducted in five urban parks and one urban forest in Kediri City. The suitability assessment was carried out through a literature review. The results showed that 29 tree species belonging to 17 families were found in the study sites, with the family Fabaceae being the most dominant one with five species. The number of exotic tree species (17) was higher than that of the native ones (12). The suitability assessment results showed that 21 tree species had a high score of 39-44, and only eight urban tree species had a medium score of 36-38. The urban parks and urban forests in Kediri were managed for the conservation of germplasm, recreation, and protection. The shapes were clumped and line. This study will contribute information to be used as guidance for urban tree management and policy framework.","PeriodicalId":19481,"journal":{"name":"Nusantara Bioscience","volume":" ","pages":""},"PeriodicalIF":0.4,"publicationDate":"2021-07-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"43662001","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2021-07-07DOI: 10.13057/nusbiosci/n130202
P. Apridamayanti, R. Sari, Anita Rachmaningtyas, Vella Aranthi
Abstract. Apridamayanti P, Sari R, Rachmaningtyas A, Aranthi V. 2021. Antioxidant, antibacterial activity and FICI (Fractional Inhibitory Concentration Index) of ethanolic extract of Melastoma malabathricum leaves with amoxicillin against pathogenic bacteria. Nusantara Bioscience 13: 140-147. Cengkodok plants (Melastoma malabathricum L.) from the Melastomataceae tribe have properties as a fever reliever (antipyretic), pain reliever (analgesic), relieve swelling, and treat burns or bleeding wounds. This study aimed to determine the antioxidant activity, total phenol content, total flavonoids and, MIC value of ethanol extract of M. malabathricum leaves against Bacillus cereus, Bacillus subtilis, Staphylococcus aureus, Staphylococcus epidermidis, Escherichia coli, Proteus mirabilis, and Pseudomonas aeruginosa. The method used in this study was the antioxidant activity was carried out using DPPH test, the total phenol-flavonoid content by using gallic-quercetin acid comparison and measurement with Spectrophotometry UV/Vis. Antibacterial activity was carried out by the Kirby-Bauer disc diffusion method. Antioxidant activity in this study showed is IC50 value = 5.28µg/mL. Chemical constituents total phenols are 36.645 ± 5.14%, and total flavonoids is 4.163 ± 0.2%. Antibacterial activity MIC values ??obtained at a concentration of 6.25 mg/mL against B. cereus with a diameter of 8.28 mm ± 0.225, against B. subtilis with a diameter of 8.53 mm ± 0.275, against S. aureus with a diameter of 7.23 mm ± 0.275, against S. epidermidis with a diameter of 6.18 mm ± 0.104; MIC values ??obtained at a concentration of 1.56 mg/mL in E. coli with a diameter of inhibition zone 6.77 ± 0.72 mm, P. mirabilis with a diameter of inhibition zone 6.32 ± 0.19 mm, and P. aeruginosa with a diameter of inhibition zone 6.40 ± 0.26 mm. The characteristic of the combination of c leaf ethanol extract, and Amoxicillin towards S. epidermidis and B. subtilis with FICI value is one categories additive; P. aeruginosa with FICI value is two categories indifferent; E. coli, B. cereus, S. aureus, and P. mirabilis with FICI value is 0.5 categories synergistic.
{"title":"Antioxidant, antibacterial activity and FICI (Fractional Inhibitory Concentration Index) of ethanolic extract of Melastoma malabathricum leaves with amoxicillin against pathogenic bacteria","authors":"P. Apridamayanti, R. Sari, Anita Rachmaningtyas, Vella Aranthi","doi":"10.13057/nusbiosci/n130202","DOIUrl":"https://doi.org/10.13057/nusbiosci/n130202","url":null,"abstract":"Abstract. Apridamayanti P, Sari R, Rachmaningtyas A, Aranthi V. 2021. Antioxidant, antibacterial activity and FICI (Fractional Inhibitory Concentration Index) of ethanolic extract of Melastoma malabathricum leaves with amoxicillin against pathogenic bacteria. Nusantara Bioscience 13: 140-147. Cengkodok plants (Melastoma malabathricum L.) from the Melastomataceae tribe have properties as a fever reliever (antipyretic), pain reliever (analgesic), relieve swelling, and treat burns or bleeding wounds. This study aimed to determine the antioxidant activity, total phenol content, total flavonoids and, MIC value of ethanol extract of M. malabathricum leaves against Bacillus cereus, Bacillus subtilis, Staphylococcus aureus, Staphylococcus epidermidis, Escherichia coli, Proteus mirabilis, and Pseudomonas aeruginosa. The method used in this study was the antioxidant activity was carried out using DPPH test, the total phenol-flavonoid content by using gallic-quercetin acid comparison and measurement with Spectrophotometry UV/Vis. Antibacterial activity was carried out by the Kirby-Bauer disc diffusion method. Antioxidant activity in this study showed is IC50 value = 5.28µg/mL. Chemical constituents total phenols are 36.645 ± 5.14%, and total flavonoids is 4.163 ± 0.2%. Antibacterial activity MIC values ??obtained at a concentration of 6.25 mg/mL against B. cereus with a diameter of 8.28 mm ± 0.225, against B. subtilis with a diameter of 8.53 mm ± 0.275, against S. aureus with a diameter of 7.23 mm ± 0.275, against S. epidermidis with a diameter of 6.18 mm ± 0.104; MIC values ??obtained at a concentration of 1.56 mg/mL in E. coli with a diameter of inhibition zone 6.77 ± 0.72 mm, P. mirabilis with a diameter of inhibition zone 6.32 ± 0.19 mm, and P. aeruginosa with a diameter of inhibition zone 6.40 ± 0.26 mm. The characteristic of the combination of c leaf ethanol extract, and Amoxicillin towards S. epidermidis and B. subtilis with FICI value is one categories additive; P. aeruginosa with FICI value is two categories indifferent; E. coli, B. cereus, S. aureus, and P. mirabilis with FICI value is 0.5 categories synergistic.","PeriodicalId":19481,"journal":{"name":"Nusantara Bioscience","volume":" ","pages":""},"PeriodicalIF":0.4,"publicationDate":"2021-07-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"47285423","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2021-07-06DOI: 10.13057/nusbiosci/n130117
Mohammed Hasan Barrak, M. Majeed, Z. Motaweq
Abstract. Barrak MH, AL-Rufaie MM, Motaweq ZY. 2021. Thymol quantitative analysis in medicinal formulation types through employing nano-technology and antimicrobial activity in some pathogenic bacterial isolates. Nusantara Bioscience 13: 129-137. This study included a method for estimating thymol (THY) in its pure state and in some of its pharmaceutical preparations that were quick, easy, and sensitive, This method is based on nanoparticles that have been modified by oxidation and reduction reactions, In a sodium hydroxide base medium, with polyvinylpyrrolidone as a stabilizer. The thymol drug works as a reducing agent to dilute the ore mineral salt from silver nitrate (Ag+) to silver nanoparticles; the oxidation-reduction reaction product for thymol has the highest absorption at 410 nm. The calibration curve was calculated, and the following information was determined, indicating that the Beer-Lambert Law was followed within the focus range of 0.25 to 50 parts per million. Sandal's sensitivity was 0.052 ?g/cm2, his molar absorptivity was 2.2883 x 103 L / mol.cm, his standard deviation rate was 0.402%, and his correlation coefficient was 0.9989. The biological effect on a number of Gram-negative and Gram-positive bacteria was studied, and the findings showed that the samples prepared were effective against these bacteria.
摘要Barrak MH, AL-Rufaie MM, Motaweq ZY。2021. 应用纳米技术定量分析药物剂型中的百里香酚及部分病原菌的抑菌活性。生物科学(13):129-137。本研究建立了一种快速、简便、灵敏地测定纯百里香酚及其制剂中百里香酚含量的方法。该方法是在氢氧化钠基介质中，以聚乙烯吡咯烷酮为稳定剂，通过氧化和还原反应修饰纳米颗粒。百里香酚类药物作为还原剂将硝酸银(Ag+)的矿物盐稀释为纳米银;百里香酚的氧化还原反应产物在410 nm处吸收最高。计算校准曲线，并确定以下信息，表明在0.25至50百万分之一的聚焦范围内符合比尔-朗伯定律。桑达尔的灵敏度为0.052 μ g/cm2，摩尔吸光度为2.2883 × 103 L / mol.cm，标准差为0.402%，相关系数为0.9989。对革兰氏阴性菌和革兰氏阳性菌的生物学效应进行了研究，结果表明制备的样品对革兰氏阴性菌和革兰氏阳性菌均有较好的抑菌效果。
{"title":"Thymol quantitative analysis in medicinal formulation types through employing of nano-technology and antimicrobial activity in some pathogenic bacterial isolates","authors":"Mohammed Hasan Barrak, M. Majeed, Z. Motaweq","doi":"10.13057/nusbiosci/n130117","DOIUrl":"https://doi.org/10.13057/nusbiosci/n130117","url":null,"abstract":"Abstract. Barrak MH, AL-Rufaie MM, Motaweq ZY. 2021. Thymol quantitative analysis in medicinal formulation types through employing nano-technology and antimicrobial activity in some pathogenic bacterial isolates. Nusantara Bioscience 13: 129-137. This study included a method for estimating thymol (THY) in its pure state and in some of its pharmaceutical preparations that were quick, easy, and sensitive, This method is based on nanoparticles that have been modified by oxidation and reduction reactions, In a sodium hydroxide base medium, with polyvinylpyrrolidone as a stabilizer. The thymol drug works as a reducing agent to dilute the ore mineral salt from silver nitrate (Ag+) to silver nanoparticles; the oxidation-reduction reaction product for thymol has the highest absorption at 410 nm. The calibration curve was calculated, and the following information was determined, indicating that the Beer-Lambert Law was followed within the focus range of 0.25 to 50 parts per million. Sandal's sensitivity was 0.052 ?g/cm2, his molar absorptivity was 2.2883 x 103 L / mol.cm, his standard deviation rate was 0.402%, and his correlation coefficient was 0.9989. The biological effect on a number of Gram-negative and Gram-positive bacteria was studied, and the findings showed that the samples prepared were effective against these bacteria.\u0000 ","PeriodicalId":19481,"journal":{"name":"Nusantara Bioscience","volume":" ","pages":""},"PeriodicalIF":0.4,"publicationDate":"2021-07-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"43196706","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2021-06-08DOI: 10.13057/nusbiosci/n130116
Janat Gul, S. Saeed, Alia Ahmed, S. Laghari, Abdul Basit, Abdul Rehman, M. Zahid
Abstract. Gul J, Saeed S, Ahmed A, Leghari SK, Basit A, Rehman A, Khan MZ. 2021. Genetic diversity and morphological variation of Pinus gerardiana along the environmental gradient from Zhob, Balochistan, Pakistan. Nusantara Bioscience 13: 121-128. Pinus gerardiana, an evergreen gymnosperm, is an economically- and ecologically valuable tree found in the Takhte Suleman Mountain Range, Zhob northeastern edge of the Balochistan, Pakistan. The present study is based on the assessment of the impact of environmental gradients with special reference to altitudinal gradient and soil variables on morphological, phytochemicals and genetic variation of P. gerardiana. A total of 27 genotypes of P. gerardiana were collected from the three elevation zones ranging from 2000 - 3500 m above sea level. The genetic diversity was assessed by combined markers, the random amplified polymorphic DNA (RAPD) and the Inter Simple Sequence Repeat (ISSR). Polymorphic bands analyzed the data to generate a dendrogram, based on the unweighted pair group method with arithmetic mean (UPGMA). Morphological characters assessed the morphological characters. Phytochemicals were investigated; the total phenolic content and total flavonoid contents were estimated and compared amongst all accessions. Our results revealed variations along altitudinal gradients and related to soil characteristics. The populations at the Middle altitude zone have greater diversity than populations at lower and higher altitudes. The resulting altitudinal variation can be attributed to various geographical and environmental conditions. These results can help in conservation and cultivation of this economically important tree.
{"title":"Genetic diversity and morphological variation of Pinus gerardiana along the environmental gradient from Zhob, Balochistan, Pakistan","authors":"Janat Gul, S. Saeed, Alia Ahmed, S. Laghari, Abdul Basit, Abdul Rehman, M. Zahid","doi":"10.13057/nusbiosci/n130116","DOIUrl":"https://doi.org/10.13057/nusbiosci/n130116","url":null,"abstract":"Abstract. Gul J, Saeed S, Ahmed A, Leghari SK, Basit A, Rehman A, Khan MZ. 2021. Genetic diversity and morphological variation of Pinus gerardiana along the environmental gradient from Zhob, Balochistan, Pakistan. Nusantara Bioscience 13: 121-128. Pinus gerardiana, an evergreen gymnosperm, is an economically- and ecologically valuable tree found in the Takhte Suleman Mountain Range, Zhob northeastern edge of the Balochistan, Pakistan. The present study is based on the assessment of the impact of environmental gradients with special reference to altitudinal gradient and soil variables on morphological, phytochemicals and genetic variation of P. gerardiana. A total of 27 genotypes of P. gerardiana were collected from the three elevation zones ranging from 2000 - 3500 m above sea level. The genetic diversity was assessed by combined markers, the random amplified polymorphic DNA (RAPD) and the Inter Simple Sequence Repeat (ISSR). Polymorphic bands analyzed the data to generate a dendrogram, based on the unweighted pair group method with arithmetic mean (UPGMA). Morphological characters assessed the morphological characters. Phytochemicals were investigated; the total phenolic content and total flavonoid contents were estimated and compared amongst all accessions. Our results revealed variations along altitudinal gradients and related to soil characteristics. The populations at the Middle altitude zone have greater diversity than populations at lower and higher altitudes. The resulting altitudinal variation can be attributed to various geographical and environmental conditions. These results can help in conservation and cultivation of this economically important tree.","PeriodicalId":19481,"journal":{"name":"Nusantara Bioscience","volume":" ","pages":""},"PeriodicalIF":0.4,"publicationDate":"2021-06-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"45075892","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2021-04-29DOI: 10.13057/NUSBIOSCI/N130114
R. B. D. Saura, Geneveve C. Falcasantos, R. J. M. Andante, Luna C. Munda, Marycris M. Alimorong, B. J. Hernando
Saura EBD, Falcasantos GC, Andante RJM, Munda LC, Alimorong MM, Hernando BJ. 2021. Evaluation of fluctuating asymmetry and sexual dimorphism of Channa striata using landmark-based geometric morphometric analysis from Agusan Marsh and Lake Mainit in Caraga Region, Philippines. Nusantara Bioscience 13: 100-110. Evaluation was conducted to determine the impact of ecological condition to Channa striata using fluctuating asymmetry (FA) employing the landmark-based geometric morphometric analysis, using TPS software, SAGE, PAST-Hotellings Test, including Physico-chemical analysis, cyanide and four base metals. Results of Physico-chemical analysis of the two habitats showed Agusan marsh with low oxygen level (3.37-4.55) and high in Iron and TDS as compared to DENR Standards. The statistical results showed high variations (p < 0.0001) on left and right-side having percentage of 79.22% in Agusan Marsh and 72.35% in Lake Mainit which is relatively high Fluctuating Asymmetry. In terms of sex, Agusan marsh had 84.27% and 85.41% FA in males and females while Lake Mainit having 85.12% and 80.89% in males and females. The fairly similar percentage of Fluctuating Asymmetry confirms no sexual dimorphism and no shape differences between male and female, and no peculiar characters to distinguished between sexes based on the 17 landmark points. Present study concluded that FA observed in the snout tip was due to breeding behavior, the landmark points in cephalic, pectoral, and caudal region were associated with burrowing and mobility factors within the lateral line system in these fishes which thrive in high turbid habitat and low oxygen. Furthermore, it was found that the level of FA tends to increase in disturbed environs.
{"title":"Evaluation of fluctuating asymmetry and sexual dimorphism of Channa striata using landmark-based geometric morphometric analysis from Agusan Marsh and Lake Mainit in Caraga Region, Philippines","authors":"R. B. D. Saura, Geneveve C. Falcasantos, R. J. M. Andante, Luna C. Munda, Marycris M. Alimorong, B. J. Hernando","doi":"10.13057/NUSBIOSCI/N130114","DOIUrl":"https://doi.org/10.13057/NUSBIOSCI/N130114","url":null,"abstract":"Saura EBD, Falcasantos GC, Andante RJM, Munda LC, Alimorong MM, Hernando BJ. 2021. Evaluation of fluctuating asymmetry and sexual dimorphism of Channa striata using landmark-based geometric morphometric analysis from Agusan Marsh and Lake Mainit in Caraga Region, Philippines. Nusantara Bioscience 13: 100-110. Evaluation was conducted to determine the impact of ecological condition to Channa striata using fluctuating asymmetry (FA) employing the landmark-based geometric morphometric analysis, using TPS software, SAGE, PAST-Hotellings Test, including Physico-chemical analysis, cyanide and four base metals. Results of Physico-chemical analysis of the two habitats showed Agusan marsh with low oxygen level (3.37-4.55) and high in Iron and TDS as compared to DENR Standards. The statistical results showed high variations (p < 0.0001) on left and right-side having percentage of 79.22% in Agusan Marsh and 72.35% in Lake Mainit which is relatively high Fluctuating Asymmetry. In terms of sex, Agusan marsh had 84.27% and 85.41% FA in males and females while Lake Mainit having 85.12% and 80.89% in males and females. The fairly similar percentage of Fluctuating Asymmetry confirms no sexual dimorphism and no shape differences between male and female, and no peculiar characters to distinguished between sexes based on the 17 landmark points. Present study concluded that FA observed in the snout tip was due to breeding behavior, the landmark points in cephalic, pectoral, and caudal region were associated with burrowing and mobility factors within the lateral line system in these fishes which thrive in high turbid habitat and low oxygen. Furthermore, it was found that the level of FA tends to increase in disturbed environs.","PeriodicalId":19481,"journal":{"name":"Nusantara Bioscience","volume":" ","pages":""},"PeriodicalIF":0.4,"publicationDate":"2021-04-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"42065966","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2021-04-16DOI: 10.13057/NUSBIOSCI/N130113
H. Lelloltery, M. Rumanta, R. M. Kunda
Abstract. Lelloltery H, Rumanta M, Kunda RM. 2021. Strategy for marine ecotourism development based on natural resource management: Case study in Kotania Bay, Western Seram District, Maluku. Nusantara Bioscience 13: 91-99. The study aimed to identify the potential for marine ecotourism in Kotania Bay, and formulated strategies and priorities for developing marine ecotourism in Kotania Bay. Data collection on ecological potential consisted of mangrove and fauna vegetation using the line transect method, while coastal and marine resources included coral reefs and reef fish using the Line Intercept Transect (LIT) method. Development strategies and priorities used the SWOT and AHP methods. The results showed that the potential of coastal natural resources around the Kotania Bay consisted of 12 mangrove species belonging to7 families with species diversity index 1.76, and dominated by Rhizophora mucronata and Bruguiera cylindrica. The bird species were 33 species belonging to 23 families, with several endemic species such as Philemon subcorniculatus, Eulipoa wallacei, Birgus latro, and Pteropus melanopogon. Potential marine water resources consisted of 45 coral species belonging to 23 genera and 14 families with 10-12 life-forms. The diversity of reef fish species was quite abundant consisting of 129 species from 60 genera and 22 families. The sand beach on Marsegu island is a tourist destination that is demanded by the tourists. SWOT analysis showed that the potential of coastal and marine natural resources in Kotania Bay is very likely to be developed as marine ecotourism.
{"title":"Strategy for marine ecotourism development based on natural resource management: Case study in Kotania Bay, Western Seram District, Maluku, Indonesia","authors":"H. Lelloltery, M. Rumanta, R. M. Kunda","doi":"10.13057/NUSBIOSCI/N130113","DOIUrl":"https://doi.org/10.13057/NUSBIOSCI/N130113","url":null,"abstract":"Abstract. Lelloltery H, Rumanta M, Kunda RM. 2021. Strategy for marine ecotourism development based on natural resource management: Case study in Kotania Bay, Western Seram District, Maluku. Nusantara Bioscience 13: 91-99. The study aimed to identify the potential for marine ecotourism in Kotania Bay, and formulated strategies and priorities for developing marine ecotourism in Kotania Bay. Data collection on ecological potential consisted of mangrove and fauna vegetation using the line transect method, while coastal and marine resources included coral reefs and reef fish using the Line Intercept Transect (LIT) method. Development strategies and priorities used the SWOT and AHP methods. The results showed that the potential of coastal natural resources around the Kotania Bay consisted of 12 mangrove species belonging to7 families with species diversity index 1.76, and dominated by Rhizophora mucronata and Bruguiera cylindrica. The bird species were 33 species belonging to 23 families, with several endemic species such as Philemon subcorniculatus, Eulipoa wallacei, Birgus latro, and Pteropus melanopogon. Potential marine water resources consisted of 45 coral species belonging to 23 genera and 14 families with 10-12 life-forms. The diversity of reef fish species was quite abundant consisting of 129 species from 60 genera and 22 families. The sand beach on Marsegu island is a tourist destination that is demanded by the tourists. SWOT analysis showed that the potential of coastal and marine natural resources in Kotania Bay is very likely to be developed as marine ecotourism.","PeriodicalId":19481,"journal":{"name":"Nusantara Bioscience","volume":" ","pages":""},"PeriodicalIF":0.4,"publicationDate":"2021-04-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"48997465","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2021-04-16DOI: 10.13057/NUSBIOSCI/N130112
M. Shahi, S. Jaberhashemi, A. Hanafi-Bojd, M. Akbari, J. Rafinejad
Shahi M, Jaberhashemi SA, Hanafi-Bojd AA, Akbari M, Rafinejad J. 2020. Faunistic study on scorpions and their health impact in Bashagard County, Hormozgan Province, Southern Iran. Nusantara Bioscience 13: 85-90. Scorpion sting is a major public health problem in tropical and subtropical countries, endangering thousands of lives annually. About 2300 scorpion sting cases including several deaths are reported from Hormozgan Province annually. This study aimed to determine the fauna of scorpions and epidemiological aspects of scorpion sting in one of the high-risk areas in this province. Scorpions were collected by diurnal searching and night catch using UV light during 2015-2016. Clinical and demographic data of scorpion sting were obtained from Hormozgan Health Center using the checklist of the Center for Disease Control (CDC) archive during the study period. Data analysis was performed using SPSS 21 software. A total of 382 scorpions comprising of 9 species belonging to Buthidae (76%) and Hemiscorpiidae (24%) families were collected and identified as Mesobuthus phillipsi, Androctonus crassicauda, Hottentotta sistanensis, Compsobuthus persicus, Hemiscorpius acanthocercus, Orthochirus farzanpayi, H. acanthocercus, and Odontobuthos sp. The most abundant species in the study area was M. phillipsi. During 2015-2016, a total of 1221 scorpion sting cases including four deaths were recorded in Bashagard County, while most of the cases occurring during summer. The climate of the eastern regions of Hormozgan Province provides a suitable habitat for one of the deadliest genera of scorpions, i.e. Hemiscorpius. This genus is the main cause of death due to scorpion sting in these regions. It is thus necessary to design appropriate programs for the prevention and treatment of scorpion sting, including health education programs for both community members and healthcare personnel.
{"title":"Faunistic study on scorpions and their health impact in Bashagard County, Hormozgan Province, Southern Iran","authors":"M. Shahi, S. Jaberhashemi, A. Hanafi-Bojd, M. Akbari, J. Rafinejad","doi":"10.13057/NUSBIOSCI/N130112","DOIUrl":"https://doi.org/10.13057/NUSBIOSCI/N130112","url":null,"abstract":"Shahi M, Jaberhashemi SA, Hanafi-Bojd AA, Akbari M, Rafinejad J. 2020. Faunistic study on scorpions and their health impact in Bashagard County, Hormozgan Province, Southern Iran. Nusantara Bioscience 13: 85-90. Scorpion sting is a major public health problem in tropical and subtropical countries, endangering thousands of lives annually. About 2300 scorpion sting cases including several deaths are reported from Hormozgan Province annually. This study aimed to determine the fauna of scorpions and epidemiological aspects of scorpion sting in one of the high-risk areas in this province. Scorpions were collected by diurnal searching and night catch using UV light during 2015-2016. Clinical and demographic data of scorpion sting were obtained from Hormozgan Health Center using the checklist of the Center for Disease Control (CDC) archive during the study period. Data analysis was performed using SPSS 21 software. A total of 382 scorpions comprising of 9 species belonging to Buthidae (76%) and Hemiscorpiidae (24%) families were collected and identified as Mesobuthus phillipsi, Androctonus crassicauda, Hottentotta sistanensis, Compsobuthus persicus, Hemiscorpius acanthocercus, Orthochirus farzanpayi, H. acanthocercus, and Odontobuthos sp. The most abundant species in the study area was M. phillipsi. During 2015-2016, a total of 1221 scorpion sting cases including four deaths were recorded in Bashagard County, while most of the cases occurring during summer. The climate of the eastern regions of Hormozgan Province provides a suitable habitat for one of the deadliest genera of scorpions, i.e. Hemiscorpius. This genus is the main cause of death due to scorpion sting in these regions. It is thus necessary to design appropriate programs for the prevention and treatment of scorpion sting, including health education programs for both community members and healthcare personnel.","PeriodicalId":19481,"journal":{"name":"Nusantara Bioscience","volume":" ","pages":""},"PeriodicalIF":0.4,"publicationDate":"2021-04-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"43934592","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2021-02-20DOI: 10.13057/NUSBIOSCI/N130109
S. Weerakoon, S. Somaratne
Abstract. Weerakoon SR, Somaratne S. 2021. Development of a core collection from Sri Lankan traditional rice (Oryza sativa) varieties for phenotypic and genetic diversity. Nusantara Bioscience 13: 61-67. A collection of over 2000 traditional rice varieties are conserved at Gene Bank, Plant Genetics Resource Center, Sri Lanka. Oryza sativa varieties grown in Sri Lanka from ancient times to the middle of the last century are known as traditional rice. These varieties show adaptability to biotic and abiotic stresses and, an important component of biodiversity of Sri Lanka. A detailed understanding of the diversity of traditional rice varieties is essential for effective utilization of rice genetic resources and identification of potential parents possessing valuable genetic traits for future crop improvement. Study objectives were phenotypic and molecular characterization of one-hundred traditional rice varieties and to identify a core collection for phenotypic and genetic diversity. Rice varieties were grown in a plant house following RCBD with 4 replicates and 5 plants per replicate. Thirty-two agro-morphological characters were observed/collected. Genomic DNA was extracted from 20-days-old seedlings. Thirty?three microsatellite (Simple Sequence Repeat-SSR) primer pairs were used to assay genetic variation and PCR products were subjected to fragment analysis by capillary electrophoresis. Descriptive statistics and basic inferential statistical analyses were performed to access variation of agro-morphological characters among rice varieties. Cluster analysis and Multidimensional scaling produced 07 groups which were further analyzed using Classification and Regression Analysis to extract the diagnostic agro-morphological features. Groups of rice varieties were characterized by lemma palea color, awn color at maturity, seedling height, and flag-leaf angle. Traditional varieties represent distant clusters on agro-morphological features. Molecular analyses revealed all 33 loci displayed polymorphism (66.7-96.9%) among 100 traditional rice varieties with a total of 387 alleles identified with an average of 11.72 alleles per variety. All varieties were genetically structured into fifteen well-separated groups. UPGMA analysis based on Jaccard's similarity separated varieties into 05 major clusters. Genetic diversity information is useful in the efficient use of Sri Lankan rice germplasm and managing in situ and ex situ germplasm collections in conserving traditional rice varieties.
{"title":"Development of a core collection from Sri Lankan traditional rice (Oryza sativa) varieties for phenotypic and genetic diversity","authors":"S. Weerakoon, S. Somaratne","doi":"10.13057/NUSBIOSCI/N130109","DOIUrl":"https://doi.org/10.13057/NUSBIOSCI/N130109","url":null,"abstract":"Abstract. Weerakoon SR, Somaratne S. 2021. Development of a core collection from Sri Lankan traditional rice (Oryza sativa) varieties for phenotypic and genetic diversity. Nusantara Bioscience 13: 61-67. A collection of over 2000 traditional rice varieties are conserved at Gene Bank, Plant Genetics Resource Center, Sri Lanka. Oryza sativa varieties grown in Sri Lanka from ancient times to the middle of the last century are known as traditional rice. These varieties show adaptability to biotic and abiotic stresses and, an important component of biodiversity of Sri Lanka. A detailed understanding of the diversity of traditional rice varieties is essential for effective utilization of rice genetic resources and identification of potential parents possessing valuable genetic traits for future crop improvement. Study objectives were phenotypic and molecular characterization of one-hundred traditional rice varieties and to identify a core collection for phenotypic and genetic diversity. Rice varieties were grown in a plant house following RCBD with 4 replicates and 5 plants per replicate. Thirty-two agro-morphological characters were observed/collected. Genomic DNA was extracted from 20-days-old seedlings. Thirty?three microsatellite (Simple Sequence Repeat-SSR) primer pairs were used to assay genetic variation and PCR products were subjected to fragment analysis by capillary electrophoresis. Descriptive statistics and basic inferential statistical analyses were performed to access variation of agro-morphological characters among rice varieties. Cluster analysis and Multidimensional scaling produced 07 groups which were further analyzed using Classification and Regression Analysis to extract the diagnostic agro-morphological features. Groups of rice varieties were characterized by lemma palea color, awn color at maturity, seedling height, and flag-leaf angle. Traditional varieties represent distant clusters on agro-morphological features. Molecular analyses revealed all 33 loci displayed polymorphism (66.7-96.9%) among 100 traditional rice varieties with a total of 387 alleles identified with an average of 11.72 alleles per variety. All varieties were genetically structured into fifteen well-separated groups. UPGMA analysis based on Jaccard's similarity separated varieties into 05 major clusters. Genetic diversity information is useful in the efficient use of Sri Lankan rice germplasm and managing in situ and ex situ germplasm collections in conserving traditional rice varieties.","PeriodicalId":19481,"journal":{"name":"Nusantara Bioscience","volume":" ","pages":""},"PeriodicalIF":0.4,"publicationDate":"2021-02-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"46271011","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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