Pub Date : 2021-02-20DOI: 10.13057/NUSBIOSCI/N130108
F. Oktavia, S. Sudarsono, D. Dinarti
Abstract. Oktavia F, Sudarsono, Dinarti D. 2021. Preliminary QTL detection for Corynespora Leaf Fall disease resistance in rubber plant. Nusantara Bioscience 13: 53-61. Corynespora Leaf Fall (CLF) disease caused by Corynespora cassiicola fungus is one of the most important diseases of rubber trees. Conventional breeding to obtain the resistant rubber clones was constrained by the long time required for selection processes. This study was aimed to identify resistant loci linked with CLF disease on the rubber tree. Analysis was conducted on 104 F1 of BPM 1 x RRIM 600. The resistance evaluation of the population had been done against two C. cassiicola isolates and the molecular analysis was generated by using 28 selected SSR markers. There was a phenotypic variation in F1 resistance to both isolates with leaf wilting intensities ranged from 5.2% to 33.4% on CC-06 isolate, and from 3.5% to 36.4% on CC-22 isolate. There was no QTL identified on the genetic linkage map, but a single marker analysis showed that some loci associated with CLF disease. The associated loci can be used as a preliminary information to develop molecular markers linked with resistance to CLF disease to assist the rubber breeding programs.
摘要Oktavia F，Sudarsono，Dinarti D.2021。橡胶植物棒孢菌落叶病抗性QTL的初步检测。Nusantara Bioscience 13:53-61。由黑胶棒孢菌引起的落叶棒孢病是橡胶树最重要的病害之一。获得抗性橡胶无性系的常规育种受到选择过程所需的长时间的限制。本研究旨在鉴定橡胶树上与CLF病相关的抗性基因座。对BPM 1 x RRIM 600的104 F1进行分析。对该群体对两个决明子菌株进行了抗性评价，并利用28个选择的SSR标记进行了分子分析。F1对两个分离株的抗性存在表型变异，CC-06分离株的叶片萎蔫强度为5.2%-33.4%，CC-22分离株的叶萎蔫程度为3.5%-36.4%。遗传连锁图上没有鉴定出QTL，但单标记分析表明，一些位点与CLF疾病有关。相关基因座可作为开发与CLF疾病抗性相关的分子标记的初步信息，以帮助橡胶育种计划。
{"title":"Preliminary QTL detection for Corynespora Leaf Fall disease resistance in rubber plant","authors":"F. Oktavia, S. Sudarsono, D. Dinarti","doi":"10.13057/NUSBIOSCI/N130108","DOIUrl":"https://doi.org/10.13057/NUSBIOSCI/N130108","url":null,"abstract":"Abstract. Oktavia F, Sudarsono, Dinarti D. 2021. Preliminary QTL detection for Corynespora Leaf Fall disease resistance in rubber plant. Nusantara Bioscience 13: 53-61. Corynespora Leaf Fall (CLF) disease caused by Corynespora cassiicola fungus is one of the most important diseases of rubber trees. Conventional breeding to obtain the resistant rubber clones was constrained by the long time required for selection processes. This study was aimed to identify resistant loci linked with CLF disease on the rubber tree. Analysis was conducted on 104 F1 of BPM 1 x RRIM 600. The resistance evaluation of the population had been done against two C. cassiicola isolates and the molecular analysis was generated by using 28 selected SSR markers. There was a phenotypic variation in F1 resistance to both isolates with leaf wilting intensities ranged from 5.2% to 33.4% on CC-06 isolate, and from 3.5% to 36.4% on CC-22 isolate. There was no QTL identified on the genetic linkage map, but a single marker analysis showed that some loci associated with CLF disease. The associated loci can be used as a preliminary information to develop molecular markers linked with resistance to CLF disease to assist the rubber breeding programs.","PeriodicalId":19481,"journal":{"name":"Nusantara Bioscience","volume":"1 1","pages":""},"PeriodicalIF":0.4,"publicationDate":"2021-02-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"42948874","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2021-02-20DOI: 10.13057/NUSBIOSCI/N130110
S. Homchan, Yash Gupta
Abstract. Homchan S, Gupta YM. 2020. Short communication: Insect detection using a machine learning model. Nusantara Bioscience 13: 69-73. The key step in characterizing any organisms and their gender highly relies on correct identification of specimens. Here we aim to classify insect and their sex by supervised machine learning (ML) model. In the present preliminary study, we used a newly developed graphical user interface (GUI) based platform to create a machine learning model for classifying two economically important cricket species. This study aims to develop ML model for Acheta domesticus and Gryllus bimaculatus species classification and sexing. An experimental investigation was conducted to use Google teachable machine GTM for preliminary cricket species detection and sexing using pre-processed 2646 still images. An alternative method for image processing is used to extract still images from high-resolution video for optimum accuracy. Out of the 2646 images, 2247 were used for training ML model and 399 were used for testing the trained model. The prediction accuracy of trained model had 100 % accuracy to identify both species and their sex. The developed trained model can be integrated into the mobile application for cricket species classification and sexing. The present study may guide professionals in the field of life science to develop ML models based on image classification, and serve as an example for researchers and taxonomists to employ machine learning for species classification and sexing in the preliminary analysis. Apart from our main goals, the paper also intends to provide the possibility of ML models in biological studies and to conduct the preliminary assessment of biodiversity.
{"title":"Short communication: Insect detection using a machine learning model","authors":"S. Homchan, Yash Gupta","doi":"10.13057/NUSBIOSCI/N130110","DOIUrl":"https://doi.org/10.13057/NUSBIOSCI/N130110","url":null,"abstract":"Abstract. Homchan S, Gupta YM. 2020. Short communication: Insect detection using a machine learning model. Nusantara Bioscience 13: 69-73. The key step in characterizing any organisms and their gender highly relies on correct identification of specimens. Here we aim to classify insect and their sex by supervised machine learning (ML) model. In the present preliminary study, we used a newly developed graphical user interface (GUI) based platform to create a machine learning model for classifying two economically important cricket species. This study aims to develop ML model for Acheta domesticus and Gryllus bimaculatus species classification and sexing. An experimental investigation was conducted to use Google teachable machine GTM for preliminary cricket species detection and sexing using pre-processed 2646 still images. An alternative method for image processing is used to extract still images from high-resolution video for optimum accuracy. Out of the 2646 images, 2247 were used for training ML model and 399 were used for testing the trained model. The prediction accuracy of trained model had 100 % accuracy to identify both species and their sex. The developed trained model can be integrated into the mobile application for cricket species classification and sexing. The present study may guide professionals in the field of life science to develop ML models based on image classification, and serve as an example for researchers and taxonomists to employ machine learning for species classification and sexing in the preliminary analysis. Apart from our main goals, the paper also intends to provide the possibility of ML models in biological studies and to conduct the preliminary assessment of biodiversity.","PeriodicalId":19481,"journal":{"name":"Nusantara Bioscience","volume":" ","pages":""},"PeriodicalIF":0.4,"publicationDate":"2021-02-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"43617124","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2021-02-20DOI: 10.13057/NUSBIOSCI/N130111
Ramzy A. Yousif, M. Choudhary, Shakel Ahmed, Quratulan Ahmed
Abstract. Yousif RA, Choudhary MI, Ahmed S, Ahmed Q. 2020. Review: Bioaccumulation of heavy metals in fish and other aquatic organisms from Karachi Coast, Pakistan. Nusantara Bioscience 13: 74-85. Heavy metals are being utilized in a variety of ways in industries, agriculture, food processing and household in many forms. Metals are unique environmental and industrial pollutants in the sense that they are neither created nor destroyed by human beings but are only transported and transformed into various products. The present study deals with the findings of various investigators on the effect of heavy metals on fish and other aquatic organisms on Karachi coasts of Pakistan. The polluted areas (Rivers and Karachi coasts) receiving effluents from industrial, agricultural, municipal and domestic wastes. The order of abundance of the metals were as fellow; Fe > Zn > Cu > Mn > Cd > Pb > Cr > Ni > Hg > As. Most studies showed that essential metals (Fe, Zn, Cu, and Mn) in aquatic organisms are much high, but the quantities of non-essential metals are found to be less. This review has shown that fish and other aquatic organisms are used as bio-monitoring species in heavy metal pollution. It is suggested that such investigations should be continuous in terms of both human health and determination of metal pollution in aquatic environment.
{"title":"Review: Bioaccumulation of heavy metals in fish and other aquatic organisms from Karachi Coast, Pakistan","authors":"Ramzy A. Yousif, M. Choudhary, Shakel Ahmed, Quratulan Ahmed","doi":"10.13057/NUSBIOSCI/N130111","DOIUrl":"https://doi.org/10.13057/NUSBIOSCI/N130111","url":null,"abstract":"Abstract. Yousif RA, Choudhary MI, Ahmed S, Ahmed Q. 2020. Review: Bioaccumulation of heavy metals in fish and other aquatic organisms from Karachi Coast, Pakistan. Nusantara Bioscience 13: 74-85. Heavy metals are being utilized in a variety of ways in industries, agriculture, food processing and household in many forms. Metals are unique environmental and industrial pollutants in the sense that they are neither created nor destroyed by human beings but are only transported and transformed into various products. The present study deals with the findings of various investigators on the effect of heavy metals on fish and other aquatic organisms on Karachi coasts of Pakistan. The polluted areas (Rivers and Karachi coasts) receiving effluents from industrial, agricultural, municipal and domestic wastes. The order of abundance of the metals were as fellow; Fe > Zn > Cu > Mn > Cd > Pb > Cr > Ni > Hg > As. Most studies showed that essential metals (Fe, Zn, Cu, and Mn) in aquatic organisms are much high, but the quantities of non-essential metals are found to be less. This review has shown that fish and other aquatic organisms are used as bio-monitoring species in heavy metal pollution. It is suggested that such investigations should be continuous in terms of both human health and determination of metal pollution in aquatic environment.","PeriodicalId":19481,"journal":{"name":"Nusantara Bioscience","volume":" ","pages":""},"PeriodicalIF":0.4,"publicationDate":"2021-02-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"47788360","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2021-01-28DOI: 10.13057/NUSBIOSCI/N130105
Alok Dhakal, S. Khanal, M. Pandey
Abstract. Dhakal A, Khanal S, Pandey M. 2021. Ethnoveterinary practice of medicinal plants in Chhatradev Rural Municipality, Arghakhanchi District of Western Nepal. Nusantara Bioscience 13: 29-40. The use of medicinal plants is a traditional system in treating domestic animals in Nepal. This study was done to investigate and document knowledge about using different plants with medicinal value to cure different animal ailments in Chhatradev Rural Municipality, Arghakhanchi district of western Nepal. A total of 100 elderly people rearing domestic animals and having traditional knowledge of ethnoveterinary plants were selected for interview. A semi-structured questionnaire was employed, and interviews were conducted for addressing detailed ethnoveterinary information. The uses of 103 plant species belonging to 56 families were documented for the treatment of 21 animal ailments. Fabaceae was found to be a dominant family with 10 plant species followed by Poaceae (8 species). The most commonly used plant parts were leaf, seed, and fruit. Herbs were dominant with 44 plant species followed by trees (32 species), shrubs (14 species), and climbers (13 species). The wide application of the medicinal plants as the form of paste formulation was observed for 41 plant species, while 25 plant species were used as powder formulation followed by Juice (21 species), raw (20 species), decoction (19 species), infusion (5 species), and roasted formulation (3 species). Oral route was the most common route followed by dermal and ocular. It was found that the informant consensus factor (ICF) values varied from 0.858 to 0.96. A high informant consensus factor was found for ailment of reproductive category (0.96) followed by respiratory (0.957), gastrointestinal (0.949) while the least was in urinary problem category (0.858). The highest citation frequency was found for Trachyspermum ammi (L.) Sprague (247) followed by Myristica fragrans Houtt. (111), Sesamum indicum L. (109), Saccharum officinarum L. (107) and Zea mays L. (97). The wide use and rich knowledge of ethnoveterinary practice were found in the study area. This study might be handy to discover useful ethnopharmaceutical agents applicable in the livestock industry. Conservation programs should be done from the government level and study on the use of medicinal plants for treating animal diseases is recommended.
{"title":"Ethnoveterinary practice of medicinal plants in Chhatradev Rural Municipality, Arghakhanchi District of Western Nepal","authors":"Alok Dhakal, S. Khanal, M. Pandey","doi":"10.13057/NUSBIOSCI/N130105","DOIUrl":"https://doi.org/10.13057/NUSBIOSCI/N130105","url":null,"abstract":"Abstract. Dhakal A, Khanal S, Pandey M. 2021. Ethnoveterinary practice of medicinal plants in Chhatradev Rural Municipality, Arghakhanchi District of Western Nepal. Nusantara Bioscience 13: 29-40. The use of medicinal plants is a traditional system in treating domestic animals in Nepal. This study was done to investigate and document knowledge about using different plants with medicinal value to cure different animal ailments in Chhatradev Rural Municipality, Arghakhanchi district of western Nepal. A total of 100 elderly people rearing domestic animals and having traditional knowledge of ethnoveterinary plants were selected for interview. A semi-structured questionnaire was employed, and interviews were conducted for addressing detailed ethnoveterinary information. The uses of 103 plant species belonging to 56 families were documented for the treatment of 21 animal ailments. Fabaceae was found to be a dominant family with 10 plant species followed by Poaceae (8 species). The most commonly used plant parts were leaf, seed, and fruit. Herbs were dominant with 44 plant species followed by trees (32 species), shrubs (14 species), and climbers (13 species). The wide application of the medicinal plants as the form of paste formulation was observed for 41 plant species, while 25 plant species were used as powder formulation followed by Juice (21 species), raw (20 species), decoction (19 species), infusion (5 species), and roasted formulation (3 species). Oral route was the most common route followed by dermal and ocular. It was found that the informant consensus factor (ICF) values varied from 0.858 to 0.96. A high informant consensus factor was found for ailment of reproductive category (0.96) followed by respiratory (0.957), gastrointestinal (0.949) while the least was in urinary problem category (0.858). The highest citation frequency was found for Trachyspermum ammi (L.) Sprague (247) followed by Myristica fragrans Houtt. (111), Sesamum indicum L. (109), Saccharum officinarum L. (107) and Zea mays L. (97). The wide use and rich knowledge of ethnoveterinary practice were found in the study area. This study might be handy to discover useful ethnopharmaceutical agents applicable in the livestock industry. Conservation programs should be done from the government level and study on the use of medicinal plants for treating animal diseases is recommended.","PeriodicalId":19481,"journal":{"name":"Nusantara Bioscience","volume":" ","pages":""},"PeriodicalIF":0.4,"publicationDate":"2021-01-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"49129752","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2021-01-28DOI: 10.13057/NUSBIOSCI/N130106
A. Alenezi, Khadiga Hassan, T. Amr, Abdulellah Alsolais
Abstract. Alenezi AK, Hassan KAG, Amr TEE, Alsolais A. 2021. Impact of cognitive-behavioral therapy on daily living skills of high functioning autistic children with anxiety disorders. Nusantara Bioscience 13: 41-46. Cognitively Behavioral Therapy (CBT) is a psychotherapeutic intervention that is used to improve mental health. It is known to have positive effect on the daily living skills of autistic children with anxiety disorders. The aim of the current study was to evaluate the effect of cognitive-behavioral therapy on daily living skills of high functioning autistic children with anxiety disorders. A Quasi-experimental research design was adopted in this research. A purposive sample of 100 autistic children (50 in intervention group and 50 in control group) aged between 7-8 years were included in this study. Three main tools were used: Vineland Adaptive Behavior Scale used to measure the everyday living skills of children, Parent-Child Interaction Questionnaire (PACHIQ), and Diagnostic and Statistical Manual of Mental Disorders (DSM-V) for assessing anxiety disorder in children and adolescents 6-18 years. The results showed an improvement in children’s daily life skills and slight reductions in caregiver’s participation in the daily life skills of children. There was a significant difference between intervention group and control groups (p < 0.001). The current results exhibit that CBT may help in increasing autistic children's independence towards daily living skills.
{"title":"Impact of cognitive-behavioral therapy on daily living skills of high functioning autistic children with anxiety disorders","authors":"A. Alenezi, Khadiga Hassan, T. Amr, Abdulellah Alsolais","doi":"10.13057/NUSBIOSCI/N130106","DOIUrl":"https://doi.org/10.13057/NUSBIOSCI/N130106","url":null,"abstract":"Abstract. Alenezi AK, Hassan KAG, Amr TEE, Alsolais A. 2021. Impact of cognitive-behavioral therapy on daily living skills of high functioning autistic children with anxiety disorders. Nusantara Bioscience 13: 41-46. Cognitively Behavioral Therapy (CBT) is a psychotherapeutic intervention that is used to improve mental health. It is known to have positive effect on the daily living skills of autistic children with anxiety disorders. The aim of the current study was to evaluate the effect of cognitive-behavioral therapy on daily living skills of high functioning autistic children with anxiety disorders. A Quasi-experimental research design was adopted in this research. A purposive sample of 100 autistic children (50 in intervention group and 50 in control group) aged between 7-8 years were included in this study. Three main tools were used: Vineland Adaptive Behavior Scale used to measure the everyday living skills of children, Parent-Child Interaction Questionnaire (PACHIQ), and Diagnostic and Statistical Manual of Mental Disorders (DSM-V) for assessing anxiety disorder in children and adolescents 6-18 years. The results showed an improvement in children’s daily life skills and slight reductions in caregiver’s participation in the daily life skills of children. There was a significant difference between intervention group and control groups (p < 0.001). The current results exhibit that CBT may help in increasing autistic children's independence towards daily living skills.","PeriodicalId":19481,"journal":{"name":"Nusantara Bioscience","volume":" ","pages":""},"PeriodicalIF":0.4,"publicationDate":"2021-01-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"48160819","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2021-01-28DOI: 10.13057/NUSBIOSCI/N130107
D. P. Virgianti
Abstract. Virgianti DP. 2020. Short Communication: Serratia rubidaea as contaminant in laboratory environment. Nusantara Bioscience 13: 47-51. There have been many cases of bacterial contamination in the laboratory. The bacterial genera identified as contaminants are Bacillus, Staphylococcus, Micrococcus, Pseudomonas, Shigella and Serratia. These bacteria are classified as non-pathogenic and pathogenic bacteria that can interfere with the test and potentially develop false-positive results. The present research has shown that red-colored contaminant bacteria develop in unused sterile media in our laboratory. Based on related information, Serratia marcescens is a red bacterial species that have been reported as a contaminant in the laboratory. The purpose of this study was to identifiy contaminant bacteria at the molecular level. Based on the phylogenetic characterization using the 16S rDNA gene region, this red contaminant bacterium was identified as Serratia rubidaea.
{"title":"Short Communication: Serratia rubidaea as contaminant in laboratory environment","authors":"D. P. Virgianti","doi":"10.13057/NUSBIOSCI/N130107","DOIUrl":"https://doi.org/10.13057/NUSBIOSCI/N130107","url":null,"abstract":"Abstract. Virgianti DP. 2020. Short Communication: Serratia rubidaea as contaminant in laboratory environment. Nusantara Bioscience 13: 47-51. There have been many cases of bacterial contamination in the laboratory. The bacterial genera identified as contaminants are Bacillus, Staphylococcus, Micrococcus, Pseudomonas, Shigella and Serratia. These bacteria are classified as non-pathogenic and pathogenic bacteria that can interfere with the test and potentially develop false-positive results. The present research has shown that red-colored contaminant bacteria develop in unused sterile media in our laboratory. Based on related information, Serratia marcescens is a red bacterial species that have been reported as a contaminant in the laboratory. The purpose of this study was to identifiy contaminant bacteria at the molecular level. Based on the phylogenetic characterization using the 16S rDNA gene region, this red contaminant bacterium was identified as Serratia rubidaea.","PeriodicalId":19481,"journal":{"name":"Nusantara Bioscience","volume":"13 1","pages":""},"PeriodicalIF":0.4,"publicationDate":"2021-01-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41799609","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2020-12-30DOI: 10.13057/nusbiosci/n130103
D. N. Enyiukwu, A. C. Amadioha, C. Ononuju
Abstract. Enyiukwu DN, Amadioha AC, Ononuju CC. 2021. Histological aberrations and mode of damage of cowpea (Vigna unguiculata) by Colletotrichum destructivum. Nusantara Bioscience 13: 16-23. Leaf and stem of healthy 2-week old cowpea (Vigna unguiculata L. Walp.) seedlings were inoculated with spore suspension of the Colletotrichum destructivum O’Gara. Sections of the infection courts were prepared and examined under digital microscope. The results showed that the infection process began 12 hours after inoculation (hai) with the germination of spores of the fungus. Large multi-lobed primary hypha (somewhat spherical in shape) developed from the infection peg and remained inside a single epithelial cell for about 3 days. Towards the end of this time, the primary hyphae developed thin filamentous tentacles that punctured and branched into adjoining cells, initiating necrotrophic phase of the disease. During this period, typical anthracnose symptoms began to develop on the infected organs of the crop. The entire infection process lasted a maximum of 96 h, at the end of which acervulus that bore a single seta emerged on infected crop lesions. The mechanism of damage of the pathogen involved intra-cellular and inter-cellular colonization of the host tissues early and late in the disease cycle. The integrity of the affected tissues' cells was compromised by passage and colonization of Colletotrichum destructivum O’Gara evidenced by lack of clear-cut middle lamella and cell boundaries. Anthracnose affected cells dispossessed of vital nutrients became turbid, devoid of turgidity and vigor. Thus, these results have strong implications for plant health management in that effective environment-compliant control of the fungus should be initiated on or before the third day following arrival of the conidia of the pathogen on the crop. Seeds are major agents of introduction of Colletotrichum spp. to disease-free locations. Findings from this study also support that strong trans-border control of seeds of the crop should be maintained since the pathogen is known to be seed-borne and demonstrates sufficient cross-infection of crop plants. Key words: Hemibiotrophy, Colletotrichum destructivum, Anthracnose, Cowpea, Hyphal colonization
摘要张建军，张建军，张建军。2013。豇豆(Vigna unguiculata)被炭疽病菌(Colletotrichum destructivum)破坏的组织学畸变和破坏方式。生物科学13:16-23。以2周龄健康豇豆(Vigna unguiculata L. Walp.)幼苗的叶和茎接种了炭疽病菌(Colletotrichum destructivum O’gara)的孢子悬浮液。制备感染法庭切片，在数码显微镜下观察。结果表明，侵染过程在接种后12 h开始，真菌孢子萌发。大的多叶初生菌丝(形状有点球形)从感染的peg发育而来，并在单个上皮细胞内停留约3天。在这段时间结束时，初级菌丝发育成细丝状触手，刺穿并分支到邻近的细胞，开始疾病的坏死阶段。在此期间，典型的炭疽病症状开始出现在作物的感染器官上。整个侵染过程持续时间最长为96 h，侵染结束后，在侵染的作物病区上出现了单穗刺蚴。病原菌的损伤机制涉及疾病周期早期和晚期宿主组织的细胞内和细胞间定植。破坏炭素病菌O’gara的传代和定植破坏了受累组织细胞的完整性，表现为缺乏清晰的中间薄片和细胞边界。炭疽病感染的细胞失去了重要的营养物质，变得浑浊，缺乏浮肿和活力。因此，这些结果对植物健康管理具有重要意义，因为对真菌的有效环境适应控制应该在病原体孢子到达作物后的第三天或之前开始。种子是炭疽病菌向无病地区传播的主要媒介。本研究的结果还支持应保持对作物种子的强有力的跨境控制，因为已知病原体是种子传播的，并且显示出对作物植物的充分交叉感染。关键词:半生物营养，炭疽菌，炭疽病，豇豆，菌丝定植
{"title":"Histological aberrations and mode of damage of cowpea (Vigna unguiculata) by Colletotrichum destructivum","authors":"D. N. Enyiukwu, A. C. Amadioha, C. Ononuju","doi":"10.13057/nusbiosci/n130103","DOIUrl":"https://doi.org/10.13057/nusbiosci/n130103","url":null,"abstract":"Abstract. Enyiukwu DN, Amadioha AC, Ononuju CC. 2021. Histological aberrations and mode of damage of cowpea (Vigna unguiculata) by Colletotrichum destructivum. Nusantara Bioscience 13: 16-23. Leaf and stem of healthy 2-week old cowpea (Vigna unguiculata L. Walp.) seedlings were inoculated with spore suspension of the Colletotrichum destructivum O’Gara. Sections of the infection courts were prepared and examined under digital microscope. The results showed that the infection process began 12 hours after inoculation (hai) with the germination of spores of the fungus. Large multi-lobed primary hypha (somewhat spherical in shape) developed from the infection peg and remained inside a single epithelial cell for about 3 days. Towards the end of this time, the primary hyphae developed thin filamentous tentacles that punctured and branched into adjoining cells, initiating necrotrophic phase of the disease. During this period, typical anthracnose symptoms began to develop on the infected organs of the crop. The entire infection process lasted a maximum of 96 h, at the end of which acervulus that bore a single seta emerged on infected crop lesions. The mechanism of damage of the pathogen involved intra-cellular and inter-cellular colonization of the host tissues early and late in the disease cycle. The integrity of the affected tissues' cells was compromised by passage and colonization of Colletotrichum destructivum O’Gara evidenced by lack of clear-cut middle lamella and cell boundaries. Anthracnose affected cells dispossessed of vital nutrients became turbid, devoid of turgidity and vigor. Thus, these results have strong implications for plant health management in that effective environment-compliant control of the fungus should be initiated on or before the third day following arrival of the conidia of the pathogen on the crop. Seeds are major agents of introduction of Colletotrichum spp. to disease-free locations. Findings from this study also support that strong trans-border control of seeds of the crop should be maintained since the pathogen is known to be seed-borne and demonstrates sufficient cross-infection of crop plants. \u0000Key words: Hemibiotrophy, Colletotrichum destructivum, Anthracnose, Cowpea, Hyphal colonization","PeriodicalId":19481,"journal":{"name":"Nusantara Bioscience","volume":" ","pages":""},"PeriodicalIF":0.4,"publicationDate":"2020-12-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"46846656","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2020-12-30DOI: 10.13057/nusbiosci/n130104
azam haeidari, D. Keshavarzi, P. Owlia, H. Vatandoost, Atiyeh Rafinejad, Rafinejad Javad
Abstract. Haeidari A, Keshavarzi D, Owlia P, Vatandoost H, Rafinejad A, Rafinejad J. 2021. Isolation and enumeration of bacteria responsible for nosocomial infections from houseflies and determining their susceptibility to poison bait. Nusantara Bioscience 13: 24-28. Nosocomial infections represent a serious public health concern in developing countries. Houseflies are one of the most common household pests carrying different pathogenic organisms. The purpose of this study was to isolate and enumerate bacteria species from house flies and to determine their susceptibility to Agita® fly bait. Flies were collected from two hospital environments between July to December 2014, in Yazd Province of Iran. Bacterial species were isolated from the outer surfaces of flies, and Agita® efficacy was evaluated based on lethal time (LT50) after 1, 2, 4, 8, 16, and 32 minutes. Three species of bacteria (Escherichia coli, Pseudomonas aeruginosa, and Staphylococcus aureus) responsible for nosocomial infections have been isolated and enumerated from flies. Among the 30 flies collected, 17, 24, and 3 flies were contaminated with E. coli, S. aureus, and Ps. aeruginosa, respectively. The minimum number of isolated bacteria was 3 × 102 CFU/mL, whereas the highest number was 2.4 × 105 CFU/mL. The susceptibility results showed that, despite the existence of a significant effect for heterogeneity in both field and laboratory strains (p-value ? 0/05) and an increase in the mortality of houseflies during the time, there was no significant difference between two strains regarding the efficacy of Agita® against houseflies. The findings of the present study revealed and confirmed that houseflies have an important role in the spread of nosocomial infections in hospital environments, and they are susceptible to Agita® fly bait.
{"title":"Isolation and enumeration of bacteria responsible for nosocomial infections from houseflies and determining their susceptibility to poison bait","authors":"azam haeidari, D. Keshavarzi, P. Owlia, H. Vatandoost, Atiyeh Rafinejad, Rafinejad Javad","doi":"10.13057/nusbiosci/n130104","DOIUrl":"https://doi.org/10.13057/nusbiosci/n130104","url":null,"abstract":"Abstract. Haeidari A, Keshavarzi D, Owlia P, Vatandoost H, Rafinejad A, Rafinejad J. 2021. Isolation and enumeration of bacteria responsible for nosocomial infections from houseflies and determining their susceptibility to poison bait. Nusantara Bioscience 13: 24-28. Nosocomial infections represent a serious public health concern in developing countries. Houseflies are one of the most common household pests carrying different pathogenic organisms. The purpose of this study was to isolate and enumerate bacteria species from house flies and to determine their susceptibility to Agita® fly bait. Flies were collected from two hospital environments between July to December 2014, in Yazd Province of Iran. Bacterial species were isolated from the outer surfaces of flies, and Agita® efficacy was evaluated based on lethal time (LT50) after 1, 2, 4, 8, 16, and 32 minutes. Three species of bacteria (Escherichia coli, Pseudomonas aeruginosa, and Staphylococcus aureus) responsible for nosocomial infections have been isolated and enumerated from flies. Among the 30 flies collected, 17, 24, and 3 flies were contaminated with E. coli, S. aureus, and Ps. aeruginosa, respectively. The minimum number of isolated bacteria was 3 × 102 CFU/mL, whereas the highest number was 2.4 × 105 CFU/mL. The susceptibility results showed that, despite the existence of a significant effect for heterogeneity in both field and laboratory strains (p-value ? 0/05) and an increase in the mortality of houseflies during the time, there was no significant difference between two strains regarding the efficacy of Agita® against houseflies. The findings of the present study revealed and confirmed that houseflies have an important role in the spread of nosocomial infections in hospital environments, and they are susceptible to Agita® fly bait.","PeriodicalId":19481,"journal":{"name":"Nusantara Bioscience","volume":" ","pages":""},"PeriodicalIF":0.4,"publicationDate":"2020-12-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"47623554","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2020-12-24DOI: 10.13057/nusbiosci/n130102
Zahra Sadat Amiri, K. Akbarzadeh, M. Douraghi, Khosrou Abdi, Abbas Aghaei Afshar, Jasem ghaffari, Razieh shabani Kordshouli, M. Akbari, J. Rafinejad
Abstract. Amiri ZS, Akbarzadeh K, Douraghi M, Abdi KM, Afshar AA, Ghaffari J, Kordshouli RS, Akbari M, Rafinejad J. 2021. Effectiveness of maggot extractions and secretion (E/S) of Lucilia sericata in reducing wound surface in an experimental scalding burn injury. Nusantara Bioscience 13: 11-15. Lucilia sericata larvae have been successfully used as medicinal larvae for wound healing. This study was performed to evaluate the therapeutic effects of the primary ointment made from excretory-secretory substances of L.sericata larvae on experimental burn wounds on rabbits under laboratory conditions. Extraction of ES from the third stage of L.sericata larvae and antibiogram testing was done. Four rabbits were randomly divided into four groups; three standard third-grade burn wounds were created on the back of each rabbit for intervention groups (B, D) of wounds were used respectively from the original ointment made from ES and ES without accompanying material. Nitrofurazone ointment was used for the positive (C) and placebo ointment was used for the negative (A). On the 21st day, all scars of wounds in groups B and D were separated and the wound was free of infectious tissue, whereas in groups A and C that the scars were clinging to the base. The level of the ulcer was significantly lower in the E/S intervention group and the infectious tissue was not observed during the treatment of group B and D wounds. Methicillin-resistant Staphylococcus aureus (MRSA) did not show resistance to excretory-secretory substances of L. sericata larvae. The debridement and recovery process was faster in the ES-treated group and the initial ointment than in the control group. The results showed that maggot extractions and secretion (E/S) of L. sericata in reducing wound healing is appropriate for treatment.
{"title":"Effectiveness of maggot extractions and secretion (E/S) of Lucilia sericata in reducing wound surface in experimental scalding burn injury","authors":"Zahra Sadat Amiri, K. Akbarzadeh, M. Douraghi, Khosrou Abdi, Abbas Aghaei Afshar, Jasem ghaffari, Razieh shabani Kordshouli, M. Akbari, J. Rafinejad","doi":"10.13057/nusbiosci/n130102","DOIUrl":"https://doi.org/10.13057/nusbiosci/n130102","url":null,"abstract":"Abstract. Amiri ZS, Akbarzadeh K, Douraghi M, Abdi KM, Afshar AA, Ghaffari J, Kordshouli RS, Akbari M, Rafinejad J. 2021. Effectiveness of maggot extractions and secretion (E/S) of Lucilia sericata in reducing wound surface in an experimental scalding burn injury. Nusantara Bioscience 13: 11-15. Lucilia sericata larvae have been successfully used as medicinal larvae for wound healing. This study was performed to evaluate the therapeutic effects of the primary ointment made from excretory-secretory substances of L.sericata larvae on experimental burn wounds on rabbits under laboratory conditions. Extraction of ES from the third stage of L.sericata larvae and antibiogram testing was done. Four rabbits were randomly divided into four groups; three standard third-grade burn wounds were created on the back of each rabbit for intervention groups (B, D) of wounds were used respectively from the original ointment made from ES and ES without accompanying material. Nitrofurazone ointment was used for the positive (C) and placebo ointment was used for the negative (A). On the 21st day, all scars of wounds in groups B and D were separated and the wound was free of infectious tissue, whereas in groups A and C that the scars were clinging to the base. The level of the ulcer was significantly lower in the E/S intervention group and the infectious tissue was not observed during the treatment of group B and D wounds. Methicillin-resistant Staphylococcus aureus (MRSA) did not show resistance to excretory-secretory substances of L. sericata larvae. The debridement and recovery process was faster in the ES-treated group and the initial ointment than in the control group. The results showed that maggot extractions and secretion (E/S) of L. sericata in reducing wound healing is appropriate for treatment.","PeriodicalId":19481,"journal":{"name":"Nusantara Bioscience","volume":" ","pages":""},"PeriodicalIF":0.4,"publicationDate":"2020-12-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41775701","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2020-12-06DOI: 10.13057/nusbiosci/n130101
Micky Vincent, Q. Johnny, D. S. A. Adeni, N. Suhaili
Abstract. Vincent M, Johnny Q, Adeni DSA, Suhaili N. 2020. Potential of Candida glabrata from ragi as a bioethanol producer using selected carbohydrate substrates. Nusantara Bioscience 12: 1-10. The flexibility and efficiency of fermenting microorganisms to convert substrates to ethanol are important factors in achieving high bioethanol yields during ethanolic fermentation. In this study, Candida glabrata, a common yeast found in fermented food, was evaluated in terms of its capability to produce ethanol using different types of carbohydrates, which included simple saccharides (glucose, maltose, sucrose), polysaccharides (starch and cellulose) and complex carbohydrates (total sago effluent, TSE). Our results indicated that C. glabrata was able to efficiently produce ethanol from glucose at 79.84% TEY (Theoretical Ethanol Yield). The ethanol production from sucrose was low, which was only 6.44% TEY, while no ethanol was produced from maltose. Meanwhile, for complex carbohydrate substrates such as starch and cellulose, ethanol was produced only when supplementary enzymes were introduced. Simultaneous Saccharification and Fermentation (SSF) of starch dosed with amylases resulted in an ethanol yield of 55.08% TEY, whilst SSF of cellulose dosed with cellulases yielded a TEY of 31.41%. When SSF was performed on TSE dosed with amylases and cellulases, the highest ethanol production was recorded within 24 h, with a yield of 23.36% TEY. Lactic acid and acetic acid were found to be at minimal levels throughout the fermentation period, indicating an efficient ethanol conversion. A notable increase in C. glabrata biomass was observed in cultures fed with glucose, starch (with supplementary amylases), and TSE (with supplementary amylases and cellulases). The current study indicates that C. glabrata can be used for bioethanol production from glucose, polysaccharides, and complex starchy lignocellulosic substrates such as TSE via SSF.
{"title":"Potential of Candida glabrata from ragi as a bioethanol producer using selected carbohydrate substrates","authors":"Micky Vincent, Q. Johnny, D. S. A. Adeni, N. Suhaili","doi":"10.13057/nusbiosci/n130101","DOIUrl":"https://doi.org/10.13057/nusbiosci/n130101","url":null,"abstract":"Abstract. Vincent M, Johnny Q, Adeni DSA, Suhaili N. 2020. Potential of Candida glabrata from ragi as a bioethanol producer using selected carbohydrate substrates. Nusantara Bioscience 12: 1-10. The flexibility and efficiency of fermenting microorganisms to convert substrates to ethanol are important factors in achieving high bioethanol yields during ethanolic fermentation. In this study, Candida glabrata, a common yeast found in fermented food, was evaluated in terms of its capability to produce ethanol using different types of carbohydrates, which included simple saccharides (glucose, maltose, sucrose), polysaccharides (starch and cellulose) and complex carbohydrates (total sago effluent, TSE). Our results indicated that C. glabrata was able to efficiently produce ethanol from glucose at 79.84% TEY (Theoretical Ethanol Yield). The ethanol production from sucrose was low, which was only 6.44% TEY, while no ethanol was produced from maltose. Meanwhile, for complex carbohydrate substrates such as starch and cellulose, ethanol was produced only when supplementary enzymes were introduced. Simultaneous Saccharification and Fermentation (SSF) of starch dosed with amylases resulted in an ethanol yield of 55.08% TEY, whilst SSF of cellulose dosed with cellulases yielded a TEY of 31.41%. When SSF was performed on TSE dosed with amylases and cellulases, the highest ethanol production was recorded within 24 h, with a yield of 23.36% TEY. Lactic acid and acetic acid were found to be at minimal levels throughout the fermentation period, indicating an efficient ethanol conversion. A notable increase in C. glabrata biomass was observed in cultures fed with glucose, starch (with supplementary amylases), and TSE (with supplementary amylases and cellulases). The current study indicates that C. glabrata can be used for bioethanol production from glucose, polysaccharides, and complex starchy lignocellulosic substrates such as TSE via SSF.","PeriodicalId":19481,"journal":{"name":"Nusantara Bioscience","volume":"13 1","pages":""},"PeriodicalIF":0.4,"publicationDate":"2020-12-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"44807263","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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