Drought stress has negative effects on crop growth and production. Characterization of transcription factors that regulate the expression of drought-responsive genes is critical for understanding the transcriptional regulatory networks in response to drought, which facilitates the improvement of crop drought tolerance. Here, we identified an Alfin-like (AL) family gene ZmAL14 that negatively regulates drought resistance. Overexpression of ZmAL14 exhibits susceptibility to drought while mutation of ZmAL14 enhances drought resistance. An abscisic acid (ABA)-activated protein kinase ZmSnRK2.2 interacts and phosphorylates ZmAL14 at T38 residue. Knockout of ZmSnRK2.2 gene decreases drought resistance of maize. A dehydration-induced Rho-like small guanosine triphosphatase gene ZmROP8 is directly targeted and repressed by ZmAL14. Phosphorylation of ZmAL14 by ZmSnRK2.2 prevents its binding to the ZmROP8 promoter, thereby releasing the repression of ZmROP8 transcription. Overexpression of ZmROP8 stimulates peroxidase activity and reduces hydrogen peroxide accumulation after drought treatment. Collectively, our study indicates that ZmAL14 is a negative regulator of drought resistance, which can be phosphorylated by ZmSnRK2.2 through the ABA signaling pathway, thus preventing its suppression on ZmROP8 transcription during drought stress response.
{"title":"Phosphorylation of ZmAL14 by ZmSnRK2.2 regulates drought resistance through derepressing ZmROP8 expression","authors":"Yalin Wang, Jinkui Cheng, Yazhen Guo, Zhen Li, Shuhua Yang, Yu Wang, Zhizhong Gong","doi":"10.1111/jipb.13677","DOIUrl":"10.1111/jipb.13677","url":null,"abstract":"<div>\u0000 \u0000 <p>Drought stress has negative effects on crop growth and production. Characterization of transcription factors that regulate the expression of drought-responsive genes is critical for understanding the transcriptional regulatory networks in response to drought, which facilitates the improvement of crop drought tolerance. Here, we identified an Alfin-like (AL) family gene <i>ZmAL14</i> that negatively regulates drought resistance. Overexpression of <i>ZmAL14</i> exhibits susceptibility to drought while mutation of <i>ZmAL14</i> enhances drought resistance. An abscisic acid (ABA)-activated protein kinase ZmSnRK2.2 interacts and phosphorylates ZmAL14 at T38 residue. Knockout of <i>ZmSnRK2.2</i> gene decreases drought resistance of maize. A dehydration-induced Rho-like small guanosine triphosphatase gene <i>ZmROP8</i> is directly targeted and repressed by ZmAL14. Phosphorylation of ZmAL14 by ZmSnRK2.2 prevents its binding to the <i>ZmROP8</i> promoter, thereby releasing the repression of <i>ZmROP8</i> transcription. Overexpression of <i>ZmROP8</i> stimulates peroxidase activity and reduces hydrogen peroxide accumulation after drought treatment. Collectively, our study indicates that ZmAL14 is a negative regulator of drought resistance, which can be phosphorylated by ZmSnRK2.2 through the ABA signaling pathway, thus preventing its suppression on <i>ZmROP8</i> transcription during drought stress response.</p></div>","PeriodicalId":195,"journal":{"name":"Journal of Integrative Plant Biology","volume":null,"pages":null},"PeriodicalIF":9.3,"publicationDate":"2024-05-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141156966","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
As one of the most important environmental signals for plants, light plays a profound role in regulating virtually every aspect of plant growth and development. Light signals are perceived by plants via several families of photoreceptors, among which phytochromes are responsible for absorbing the red (R) and far-red (FR) wavelengths (600–750 nm), cryptochromes (CRYs) perceive the blue (B)/ultraviolet-A (UV-A) wavelengths (320–500 nm), and UV RESISTANCE LOCUS 8 (UVR8) is a recently-characterized UV-B (280–320 nm) photoreceptor. These photoreceptors perceive and transduce the light signals through intracellular signaling pathways, ultimately leading to adaptive physiological changes. Such adjustments of plant growth and development in response to their light environment are often mediated by hormone signaling pathways.
In this issue, Park et al. (2024) review how plant photomorphogenesis is regulated by two recently identified phytochemicals, karrikins (KARs) and strigolactones (SLs). Karrikins and SLs are structurally related butenolides, and recent accumulating data strongly support that light and KAR/SL signals act together to modulate plant growth and development and adaptive fitness to environmental stimulations. SUPPRESSOR OF MORE AXILLARY GROWTH 2 (MAX2) 1 (SMAX1) and SMAX1-LIKE (SMXL) proteins function as central negative regulators of KAR and SL signaling, and it was shown that SMAX1 and SMXL play a key role in integrating KAR/SL signals with light as well as other pathways to modulate plant growth and development. Another review by Qu et al. (2024) summarizes recent progress in understanding of the photoregulatory mechanisms of Arabidopsis CRY complexes. Particularly, the dual-action mechanism, including the “Lock-and-Key” and the “Liquid-Liquid Phase Separation” (LLPS) mechanisms, may explain, at least in part, the diversity of CRY-interacting proteins and CRY functions. The classical “Lock-and-Key” mechanism involves blue light-induced changes in the interactions between CRYs and their interacting proteins, while the recently proposed LLPS mechanism involves blue light-induced co-condensation of CRYs and their interacting proteins.
Abscisic acid (ABA) is a classical phytohormone that plays an important role in regulating plant growth and development as well as plant responses to environmental stresses. Light and ABA were shown to antagonistically regulate several plant responses or developmental processes, such as seed germination and stomatal movement. In this issue, Luo et al. (2024) showed that PHYTOCHROME-INTERACTING FACTOR4 (PIF4), a key negative regulator of photomorphogenesis, physically interacts with ABSCISIC ACID INSENSITIVE4 (ABI4), a pivotal transcription factor of ABA signaling, to form a transcriptional activator complex. The PIF4–ABI4 complex synergistically promotes the expression of its target genes including ABI4 itself, and 9-CIS-EPOXYCAROTENOID DIOXYGENASE 6
{"title":"Plant photobiology: From basic theoretical research to crop production improvement","authors":"Hongtao Liu, Jigang Li","doi":"10.1111/jipb.13672","DOIUrl":"10.1111/jipb.13672","url":null,"abstract":"<p>As one of the most important environmental signals for plants, light plays a profound role in regulating virtually every aspect of plant growth and development. Light signals are perceived by plants via several families of photoreceptors, among which phytochromes are responsible for absorbing the red (R) and far-red (FR) wavelengths (600–750 nm), cryptochromes (CRYs) perceive the blue (B)/ultraviolet-A (UV-A) wavelengths (320–500 nm), and UV RESISTANCE LOCUS 8 (UVR8) is a recently-characterized UV-B (280–320 nm) photoreceptor. These photoreceptors perceive and transduce the light signals through intracellular signaling pathways, ultimately leading to adaptive physiological changes. Such adjustments of plant growth and development in response to their light environment are often mediated by hormone signaling pathways.</p><p>In this issue, Park et al. (<span>2024</span>) review how plant photomorphogenesis is regulated by two recently identified phytochemicals, karrikins (KARs) and strigolactones (SLs). Karrikins and SLs are structurally related butenolides, and recent accumulating data strongly support that light and KAR/SL signals act together to modulate plant growth and development and adaptive fitness to environmental stimulations. SUPPRESSOR OF MORE AXILLARY GROWTH 2 (MAX2) 1 (SMAX1) and SMAX1-LIKE (SMXL) proteins function as central negative regulators of KAR and SL signaling, and it was shown that SMAX1 and SMXL play a key role in integrating KAR/SL signals with light as well as other pathways to modulate plant growth and development. Another review by Qu et al. (<span>2024</span>) summarizes recent progress in understanding of the photoregulatory mechanisms of Arabidopsis CRY complexes. Particularly, the dual-action mechanism, including the “Lock-and-Key” and the “Liquid-Liquid Phase Separation” (LLPS) mechanisms, may explain, at least in part, the diversity of CRY-interacting proteins and CRY functions. The classical “Lock-and-Key” mechanism involves blue light-induced changes in the interactions between CRYs and their interacting proteins, while the recently proposed LLPS mechanism involves blue light-induced co-condensation of CRYs and their interacting proteins.</p><p>Abscisic acid (ABA) is a classical phytohormone that plays an important role in regulating plant growth and development as well as plant responses to environmental stresses. Light and ABA were shown to antagonistically regulate several plant responses or developmental processes, such as seed germination and stomatal movement. In this issue, Luo et al. (<span>2024</span>) showed that PHYTOCHROME-INTERACTING FACTOR4 (PIF4), a key negative regulator of photomorphogenesis, physically interacts with ABSCISIC ACID INSENSITIVE4 (ABI4), a pivotal transcription factor of ABA signaling, to form a transcriptional activator complex. The PIF4–ABI4 complex synergistically promotes the expression of its target genes including ABI4 itself, and <i>9-CIS-EPOXYCAROTENOID DIOXYGENASE 6</i","PeriodicalId":195,"journal":{"name":"Journal of Integrative Plant Biology","volume":null,"pages":null},"PeriodicalIF":11.4,"publicationDate":"2024-05-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/jipb.13672","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141154472","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Issue information page","authors":"","doi":"10.1111/jipb.13518","DOIUrl":"https://doi.org/10.1111/jipb.13518","url":null,"abstract":"","PeriodicalId":195,"journal":{"name":"Journal of Integrative Plant Biology","volume":null,"pages":null},"PeriodicalIF":11.4,"publicationDate":"2024-05-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/jipb.13518","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141156526","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
The self-incompatibility response is a complex process that results in an intraspecific reproductive barrier, involving self- and non-self-recognition. Tian et al., (pages 986-1006) revealed a new mechanistic mode of self-incompatibility in angiosperms, in which the pistil factor S-RNase elicits a self-incompatibility response by indirectly interfering with the cytoskeletal organization of self pollen tubes, sequestering actin-binding proteins by phase separation in Petunia hybrida. The cover shows diverse genotypes of P. hybrida.
{"title":"Cover Image:","authors":"","doi":"10.1111/jipb.13519","DOIUrl":"https://doi.org/10.1111/jipb.13519","url":null,"abstract":"<p>The self-incompatibility response is a complex process that results in an intraspecific reproductive barrier, involving self- and non-self-recognition. Tian et al., (pages 986-1006) revealed a new mechanistic mode of self-incompatibility in angiosperms, in which the pistil factor S-RNase elicits a self-incompatibility response by indirectly interfering with the cytoskeletal organization of self pollen tubes, sequestering actin-binding proteins by phase separation in <i>Petunia hybrida</i>. The cover shows diverse genotypes of <i>P. hybrida</i>.</p>","PeriodicalId":195,"journal":{"name":"Journal of Integrative Plant Biology","volume":null,"pages":null},"PeriodicalIF":11.4,"publicationDate":"2024-05-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/jipb.13519","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141156513","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Yingchun Han, Qianfeng Hu, Nuo Gong, Huimin Yan, Najeeb Ullah Khan, Yanxiu Du, Hongzheng Sun, Quanzhi Zhao, Wanxi Peng, Zichao Li, Zhanying Zhang, Junzhou Li
Grain yield is determined mainly by grain number and grain weight. In this study, we identified and characterized MORE GRAINS1 (MOG1), a gene associated with grain number and grain weight in rice (Oryza sativa L.), through map-based cloning. Overexpression of MOG1 increased grain yield by 18.6%–22.3% under field conditions. We determined that MOG1, a bHLH transcription factor, interacts with OsbHLH107 and directly activates the expression of LONELY GUY (LOG), which encodes a cytokinin-activating enzyme and the cell expansion gene EXPANSIN-LIKE1 (EXPLA1), positively regulating grain number per panicle and grain weight. Natural variations in the promoter and coding regions of MOG1 between Hap-LNW and Hap-HNW alleles resulted in changes in MOG1 expression level and transcriptional activation, leading to functional differences. Haplotype analysis revealed that Hap-HNW, which results in a greater number and heavier grains, has undergone strong selection but has been poorly utilized in modern lowland rice breeding. In summary, the MOG1–OsbHLH107 complex activates LOG and EXPLA1 expression to promote cell expansion and division of young panicles through the cytokinin pathway, thereby increasing grain number and grain weight. These findings suggest that Hap-HNW could be used in strategies to breed high-yielding temperate japonica lowland rice.
{"title":"Natural variation in MORE GRAINS 1 regulates grain number and grain weight in rice","authors":"Yingchun Han, Qianfeng Hu, Nuo Gong, Huimin Yan, Najeeb Ullah Khan, Yanxiu Du, Hongzheng Sun, Quanzhi Zhao, Wanxi Peng, Zichao Li, Zhanying Zhang, Junzhou Li","doi":"10.1111/jipb.13674","DOIUrl":"10.1111/jipb.13674","url":null,"abstract":"<p>Grain yield is determined mainly by grain number and grain weight. In this study, we identified and characterized <i>MORE GRAINS1</i> (<i>MOG1</i>), a gene associated with grain number and grain weight in rice (<i>Oryza sativa</i> L.), through map-based cloning. Overexpression of <i>MOG1</i> increased grain yield by 18.6%–22.3% under field conditions. We determined that MOG1, a bHLH transcription factor, interacts with OsbHLH107 and directly activates the expression of <i>LONELY GUY</i> (<i>LOG</i>), which encodes a cytokinin-activating enzyme and the cell expansion gene <i>EXPANSIN-LIKE1</i> (<i>EXPLA1</i>), positively regulating grain number per panicle and grain weight. Natural variations in the promoter and coding regions of <i>MOG1</i> between Hap-LNW and Hap-HNW alleles resulted in changes in <i>MOG1</i> expression level and transcriptional activation, leading to functional differences. Haplotype analysis revealed that Hap-HNW, which results in a greater number and heavier grains, has undergone strong selection but has been poorly utilized in modern lowland rice breeding. In summary, the MOG1–OsbHLH107 complex activates <i>LOG</i> and <i>EXPLA1</i> expression to promote cell expansion and division of young panicles through the cytokinin pathway, thereby increasing grain number and grain weight. These findings suggest that Hap-HNW could be used in strategies to breed high-yielding temperate <i>japonica</i> lowland rice.</p>","PeriodicalId":195,"journal":{"name":"Journal of Integrative Plant Biology","volume":null,"pages":null},"PeriodicalIF":9.3,"publicationDate":"2024-05-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/jipb.13674","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141079760","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
A mechanized direct seeding of rice with less labor and water usage, has been widely adopted. However, this approach requires varieties that exhibit uniform seedling emergence. Mesocotyl elongation (ME) offers the main drive of fast emergence of rice seedlings from soils; nevertheless, its genetic basis remains unknown. Here, we identify a major rice quantitative trait locus Mesocotyl Elongation1 (qME1), an allele of the Green Revolution gene Semi-Dwarf1 (SD1), encoding GA20-oxidase for gibberellin (GA) biosynthesis. ME1 expression is strongly induced by soil depth and ethylene. When rice grains are direct-seeded in soils, the ethylene core signaling factor OsEIL1 directly promotes ME1 transcription, accelerating bioactive GA biosynthesis. The GAs further degrade the DELLA protein SLENDER RICE 1 (SLR1), alleviating its inhibition of rice PHYTOCHROME-INTERACTING FACTOR-LIKE13 (OsPIL13) to activate the downstream expansion gene OsEXPA4 and ultimately promote rice seedling ME and emergence. The ancient traits of long mesocotyl and strong emergence ability in wild rice and landrace were gradually lost in company with the Green Revolution dwarf breeding process, and an elite ME1-R allele (D349H) is found in some modern Geng varieties (long mesocotyl lengths) in northern China, which can be used in the direct seeding and dwarf breeding of Geng varieties. Furthermore, the ectopic and high expression of ME1 driven by mesocotyl-specific promoters resulted in rice plants that could be direct-seeded without obvious plant architecture or yield penalties. Collectively, we reveal the molecular mechanism of rice ME, and provide useful information for breeding new Green Revolution varieties with long mesocotyl suitable for direct-seeding practice.
{"title":"An orchestrated ethylene–gibberellin signaling cascade contributes to mesocotyl elongation and emergence of rice direct seeding","authors":"Yusong Lyu, Xinli Dong, Shipeng Niu, Ruijie Cao, Gaoneng Shao, Zhonghua Sheng, Guiai Jiao, Lihong Xie, Shikai Hu, Shaoqing Tang, Xiangjin Wei, Peisong Hu","doi":"10.1111/jipb.13671","DOIUrl":"10.1111/jipb.13671","url":null,"abstract":"<p>A mechanized direct seeding of rice with less labor and water usage, has been widely adopted. However, this approach requires varieties that exhibit uniform seedling emergence. Mesocotyl elongation (ME) offers the main drive of fast emergence of rice seedlings from soils; nevertheless, its genetic basis remains unknown. Here, we identify a major rice quantitative trait locus <i>Mesocotyl Elongation1</i> (<i>qME1</i>), an allele of the Green Revolution gene <i>Semi-Dwarf1</i> (<i>SD1</i>), encoding GA20-oxidase for gibberellin (GA) biosynthesis<i>. ME1</i> expression is strongly induced by soil depth and ethylene. When rice grains are direct-seeded in soils, the ethylene core signaling factor OsEIL1 directly promotes <i>ME1</i> transcription, accelerating bioactive GA biosynthesis. The GAs further degrade the DELLA protein SLENDER RICE 1 (SLR1), alleviating its inhibition of rice PHYTOCHROME-INTERACTING FACTOR-LIKE13 (OsPIL13) to activate the downstream expansion gene <i>OsEXPA4</i> and ultimately promote rice seedling ME and emergence. The ancient traits of long mesocotyl and strong emergence ability in wild rice and landrace were gradually lost in company with the Green Revolution dwarf breeding process, and an elite <i>ME1</i>-R allele (D349H) is found in some modern <i>Geng</i> varieties (long mesocotyl lengths) in northern China, which can be used in the direct seeding and dwarf breeding of <i>Geng</i> varieties. Furthermore, the ectopic and high expression of <i>ME1</i> driven by mesocotyl-specific promoters resulted in rice plants that could be direct-seeded without obvious plant architecture or yield penalties. Collectively, we reveal the molecular mechanism of rice ME, and provide useful information for breeding new Green Revolution varieties with long mesocotyl suitable for direct-seeding practice.</p>","PeriodicalId":195,"journal":{"name":"Journal of Integrative Plant Biology","volume":null,"pages":null},"PeriodicalIF":9.3,"publicationDate":"2024-05-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/jipb.13671","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140943481","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Calcium oscillations are induced by different stresses. Calcium-dependent protein kinases (CDPKs/CPKs) are one major group of the plant calcium decoders that are involved in various processes including drought response. Some CPKs are calcium-independent. Here, we identified ZmCPK2 as a negative regulator of drought resistance by screening an overexpression transgenic maize pool. We found that ZmCPK2 does not bind calcium, and its activity is mainly inhibited during short term abscisic acid (ABA) treatment, and dynamically changed in prolonged treatment. Interestingly, ZmCPK2 interacts with and is inhibited by calcium-dependent ZmCPK17, a positive regulator of drought resistance, which is activated by ABA. ZmCPK17 could prevent the nuclear localization of ZmCPK2 through phosphorylation of ZmCPK2T60. ZmCPK2 interacts with and phosphorylates and activates ZmYAB15, a negative transcriptional factor for drought resistance. Our results suggest that drought stress-induced Ca2+ can be decoded directly by ZmCPK17 that inhibits ZmCPK2, thereby promoting plant adaptation to water deficit.
{"title":"Ca2+-independent ZmCPK2 is inhibited by Ca2+-dependent ZmCPK17 during drought response in maize","authors":"Xiaoying Hu, Jinkui Cheng, Minmin Lu, Tingting Fang, Yujuan Zhu, Zhen Li, Xiqing Wang, Yu Wang, Yan Guo, Shuhua Yang, Zhizhong Gong","doi":"10.1111/jipb.13675","DOIUrl":"10.1111/jipb.13675","url":null,"abstract":"<div>\u0000 \u0000 <p>Calcium oscillations are induced by different stresses. Calcium-dependent protein kinases (CDPKs/CPKs) are one major group of the plant calcium decoders that are involved in various processes including drought response. Some CPKs are calcium-independent. Here, we identified ZmCPK2 as a negative regulator of drought resistance by screening an overexpression transgenic maize pool. We found that ZmCPK2 does not bind calcium, and its activity is mainly inhibited during short term abscisic acid (ABA) treatment, and dynamically changed in prolonged treatment. Interestingly, ZmCPK2 interacts with and is inhibited by calcium-dependent ZmCPK17, a positive regulator of drought resistance, which is activated by ABA. ZmCPK17 could prevent the nuclear localization of ZmCPK2 through phosphorylation of ZmCPK2T60. ZmCPK2 interacts with and phosphorylates and activates ZmYAB15, a negative transcriptional factor for drought resistance. Our results suggest that drought stress-induced Ca<sup>2+</sup> can be decoded directly by ZmCPK17 that inhibits ZmCPK2, thereby promoting plant adaptation to water deficit.</p></div>","PeriodicalId":195,"journal":{"name":"Journal of Integrative Plant Biology","volume":null,"pages":null},"PeriodicalIF":9.3,"publicationDate":"2024-05-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140943483","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Tapetum, the innermost layer of the anther wall, provides essential nutrients and materials for pollen development. Timely degradation of anther tapetal cells is a prerequisite for normal pollen development in flowering plants. Tapetal cells facilitate male gametogenesis by providing cellular contents after highly coordinated programmed cell death (PCD). Tapetal development is regulated by a transcriptional network. However, the signaling pathway(s) involved in this process are poorly understood. In this study, we report that a mitogen-activated protein kinase (MAPK) cascade composed of OsYDA1/OsYDA2-OsMKK4-OsMPK6 plays an important role in tapetal development and male gametophyte fertility. Loss of function of this MAPK cascade leads to anther indehiscence, enlarged tapetum, and aborted pollen grains. Tapetal cells in osmkk4 and osmpk6 mutants exhibit an increased presence of lipid body-like structures within the cytoplasm, which is accompanied by a delayed occurrence of PCD. Expression of a constitutively active version of OsMPK6 (CA-OsMPK6) can rescue the pollen defects in osmkk4 mutants, confirming that OsMPK6 functions downstream of OsMKK4 in this pathway. Genetic crosses also demonstrated that the MAPK cascade sporophyticly regulates pollen development. Our study reveals a novel function of rice MAPK cascade in plant male reproductive biology.
{"title":"Sporophytic control of tapetal development and pollen fertility by a mitogen-activated protein kinase cascade in rice","authors":"Jianguo Zeng, Manman Duan, Yiqing Wang, Guangtao Li, Yujing You, Jie Shi, Changhao Liu, Jinyang Zhang, Juan Xu, Shuqun Zhang, Jing Zhao","doi":"10.1111/jipb.13673","DOIUrl":"10.1111/jipb.13673","url":null,"abstract":"<div>\u0000 \u0000 <p>Tapetum, the innermost layer of the anther wall, provides essential nutrients and materials for pollen development. Timely degradation of anther tapetal cells is a prerequisite for normal pollen development in flowering plants. Tapetal cells facilitate male gametogenesis by providing cellular contents after highly coordinated programmed cell death (PCD). Tapetal development is regulated by a transcriptional network. However, the signaling pathway(s) involved in this process are poorly understood. In this study, we report that a mitogen-activated protein kinase (MAPK) cascade composed of OsYDA1/OsYDA2-OsMKK4-OsMPK6 plays an important role in tapetal development and male gametophyte fertility. Loss of function of this MAPK cascade leads to anther indehiscence, enlarged tapetum, and aborted pollen grains. Tapetal cells in <i>osmkk4</i> and <i>osmpk6</i> mutants exhibit an increased presence of lipid body-like structures within the cytoplasm, which is accompanied by a delayed occurrence of PCD. Expression of a constitutively active version of OsMPK6 (CA-OsMPK6) can rescue the pollen defects in <i>osmkk4</i> mutants, confirming that OsMPK6 functions downstream of OsMKK4 in this pathway. Genetic crosses also demonstrated that the MAPK cascade sporophyticly regulates pollen development. Our study reveals a novel function of rice MAPK cascade in plant male reproductive biology.</p></div>","PeriodicalId":195,"journal":{"name":"Journal of Integrative Plant Biology","volume":null,"pages":null},"PeriodicalIF":9.3,"publicationDate":"2024-05-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140943487","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Yuhe Bai, Shengnan Liu, Yan Bai, Zhisong Xu, Hainan Zhao, Haiming Zhao, Jinsheng Lai, Ya Liu, Weibin Song
A CRISPR/Cas12i.3-based gene editing platform is established in broomcorn millet (Panicum miliaceum) and used to create new elite germplasm for this ancient crop.
{"title":"Application of CRISPR/Cas12i.3 for targeted mutagenesis in broomcorn millet (Panicum miliaceum L.)","authors":"Yuhe Bai, Shengnan Liu, Yan Bai, Zhisong Xu, Hainan Zhao, Haiming Zhao, Jinsheng Lai, Ya Liu, Weibin Song","doi":"10.1111/jipb.13669","DOIUrl":"10.1111/jipb.13669","url":null,"abstract":"<p>A CRISPR/Cas12i.3-based gene editing platform is established in broomcorn millet (<i>Panicum miliaceum</i>) and used to create new elite germplasm for this ancient crop.\u0000\u0000 <figure>\u0000 <div><picture>\u0000 <source></source></picture><p></p>\u0000 </div>\u0000 </figure></p>","PeriodicalId":195,"journal":{"name":"Journal of Integrative Plant Biology","volume":null,"pages":null},"PeriodicalIF":9.3,"publicationDate":"2024-05-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/jipb.13669","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140829391","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Yupeng Cai, Li Chen, Xiaoqian Liu, Weiwei Yao, Wensheng Hou
Flowering time and growth period are key agronomic traits which directly affect soybean (Glycine max (L.) Merr.) adaptation to diverse latitudes and farming systems. The FLOWERING LOCUS T (FT) homologs GmFT2a and GmFT5a integrate multiple flowering regulation pathways and significantly advance flowering and maturity in soybean. Pinpointing the genes responsible for regulating GmFT2a and GmFT5a will improve our understanding of the molecular mechanisms governing growth period in soybean. In this study, we identified the Nuclear Factor Y-C (NFY-C) protein GmNF-YC4 as a novel flowering suppressor in soybean under long-day (LD) conditions. GmNF-YC4 delays flowering and maturation by directly repressing the expression of GmFT2a and GmFT5a. In addition, we found that a strong selective sweep event occurred in the chromosomal region harboring the GmNF-YC4 gene during soybean domestication. The GmNF-YC4Hap3 allele was mainly found in wild soybean (Glycine soja Siebold & Zucc.) and has been eliminated from G. max landraces and improved cultivars, which predominantly contain the GmNF-YC4Hap1 allele. Furthermore, the Gmnf-yc4 mutants displayed notably accelerated flowering and maturation under LD conditions. These alleles may prove to be valuable genetic resources for enhancing soybean adaptability to higher latitudes.
开花时间和生长期是直接影响大豆（Glycine max (L.) Merr.）适应不同纬度和耕作制度的关键农艺性状。FLOWERING LOCUS T（FT）的同源基因 GmFT2a 和 GmFT5a 整合了多种开花调控途径，显著促进了大豆的开花和成熟。准确定位负责调控 GmFT2a 和 GmFT5a 的基因将提高我们对大豆生长期分子机制的认识。在本研究中，我们发现核因子 Y-C（NFY-C）蛋白 GmNF-YC4 是大豆在长日照（LD）条件下的新型开花抑制因子。GmNF-YC4 通过直接抑制 GmFT2a 和 GmFT5a 的表达来延迟开花和成熟。此外，我们还发现，在大豆驯化过程中，GmNF-YC4 基因所在的染色体区域发生了强烈的选择性掠夺事件。GmNF-YC4Hap3 等位基因主要存在于野生大豆（Glycine soja Siebold & Zucc.）中，并已从主要含有 GmNF-YC4Hap1 等位基因的 G. max 陆生品系和改良栽培品种中消除。此外，在 LD 条件下，Gmnf-yc4 突变体的开花和成熟速度明显加快。这些等位基因可能被证明是提高大豆对高纬度地区适应性的宝贵遗传资源。
{"title":"GmNF-YC4 delays soybean flowering and maturation by directly repressing GmFT2a and GmFT5a expression","authors":"Yupeng Cai, Li Chen, Xiaoqian Liu, Weiwei Yao, Wensheng Hou","doi":"10.1111/jipb.13668","DOIUrl":"10.1111/jipb.13668","url":null,"abstract":"<p>Flowering time and growth period are key agronomic traits which directly affect soybean (<i>Glycine max</i> (L.) Merr.) adaptation to diverse latitudes and farming systems. The FLOWERING LOCUS T (FT) homologs GmFT2a and GmFT5a integrate multiple flowering regulation pathways and significantly advance flowering and maturity in soybean. Pinpointing the genes responsible for regulating <i>GmFT2a</i> and <i>GmFT5a</i> will improve our understanding of the molecular mechanisms governing growth period in soybean. In this study, we identified the Nuclear Factor Y-C (NFY-C) protein GmNF-YC4 as a novel flowering suppressor in soybean under long-day (LD) conditions. GmNF-YC4 delays flowering and maturation by directly repressing the expression of <i>GmFT2a</i> and <i>GmFT5a</i>. In addition, we found that a strong selective sweep event occurred in the chromosomal region harboring the <i>GmN</i>F-YC4 gene during soybean domestication. The <i>GmNF-YC4</i><sup><i>Hap3</i></sup> allele was mainly found in wild soybean (<i>Glycine soja</i> Siebold & Zucc.) and has been eliminated from <i>G. max</i> landraces and improved cultivars, which predominantly contain the <i>GmNF-YC4</i><sup><i>Hap1</i></sup> allele. Furthermore, the <i>Gmnf-yc4</i> mutants displayed notably accelerated flowering and maturation under LD conditions. These alleles may prove to be valuable genetic resources for enhancing soybean adaptability to higher latitudes.</p>","PeriodicalId":195,"journal":{"name":"Journal of Integrative Plant Biology","volume":null,"pages":null},"PeriodicalIF":9.3,"publicationDate":"2024-05-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/jipb.13668","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140842098","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
扫码关注我们
求助内容：
应助结果提醒方式：
京公网安备 11010802042870号