Pacific Medical Journal最新文献

{"title":"Improving prevention measures for healthcare-associated urinary tract infections","authors":"K. Sursyakova, T. Safyanova","doi":"10.34215/1609-1175-2023-1-100-102","DOIUrl":"https://doi.org/10.34215/1609-1175-2023-1-100-102","url":null,"abstract":"Aim. To develop an algorithm for a comprehensive assessment of endogenous and exogenous factors and risks of urinary tract infections (UTI) to improve prevention measures in multidisciplinary healthcare organizations.Materials and methods. All patients included in the study were divided into two groups. Group 1 was formed on the basis of the Altai Regional Hospital for War Veterans (700 medical histories). Regional Clinical Hospital provided 500 medical histories. Endogenous and exogenous risk factors were assessed in patients included in the study.Results. Following a comprehensive assessment of exogenous and endogenous risk factors of healthcare-associated UTI among patients of different ages, an algorithm for improving prevention measures has been developed.Conclusions. The algorithm of prevention measures for expert evaluation of risk factors and groups of healthcare-associated UTI enables the prevention of these infections to be improved. The creation of databases of risk factors that contribute to infection is essential for providing expert evaluation of standard operating procedures and internal quality audits of medical activities, as well as for determining the optimal prevention measures reducing the incidence of UTIs.","PeriodicalId":19705,"journal":{"name":"Pacific Medical Journal","volume":"94 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-04-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"89873978","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Anti-bacterial and anti-biofilm activity of bacteriophages against Klebsiella pneumoniae and Pseudomonas aeruginosa isolated from orthopedic patients","authors":"E. M. Gordina, S. Bozhkova, L. N. Smirnova","doi":"10.34215/1609-1175-2023-1-59-63","DOIUrl":"https://doi.org/10.34215/1609-1175-2023-1-59-63","url":null,"abstract":"Objective. To investigate the susceptibility of K. pneumoniae and P. aeruginosa to a polyvalent bacteriophage preparation and its effect on biofilm formation and the strain biofilms isolated from orthopedic patients.Materials and methods. The research sample included 50 clinical isolates of K. pneumoniae and 50 clinical isolates of P. aeruginosa. Identification was performed by MALDI-TOF-MS; antibiotic susceptibility was assessed in accordance with EUCAST v 21. Detection of carbapenemase genes was carried out by real-time PCR. The strain susceptibility to the bacteriophage was determined by a spot test; K. pneumoniae ATCC 33495 and P. aeruginosa ATCC 27853 were determined by assessing their growth curves. Biofilms of strains sensitive to bacteriophages were formed according to the O’Toole method by co-incubation of bacteria with phages. The effect of bacteriophages on 24-hour biofilms was assessed by comparing the optical density of dye extracts of bacteriophage-treated wells and control wells at 570 nm. The data were analyzed using the Statistica environment.Results. It was found that 7 (14%) of K. pneumoniae and 15 (30%) of P. aeruginosa were resistant to carbapenems. Six strains of K. pneumoniae produced NDM-cabapenemase, while four isolates of P. aeruginosa produced VIM-carbapenemases. The bacteriophage preparation under study was active against 36% and 56% of K. pneumoniae and P. aeruginosa strains, respectively. The majority of the studied strains reduced biofilm production upon co-incubation with a phage; however, a decrease in biomass of greater than 80% was observed only for P. aeruginosa. The effect of the bacteriophage on the already formed biofilms was less pronounced, despite a decrease in the biofilm biomass in 78% and 68% of K.pneumoniae and P. aeruginosa strains, respectively.Conclusion. The results obtained confirm the need for further research into the action of bacteriophages against pathogens caused by implant-associated infections and the development of bacteriophage therapy for orthopedic patients.","PeriodicalId":19705,"journal":{"name":"Pacific Medical Journal","volume":"19 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-04-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"81009057","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Bioprofiles of Staphylococcus spp. isolated from prostate secretion in men with chronic bacterial prostatitis","authors":"O. L. Kartashova, O. A. Pashinina, T. M. Pashkova, V. A. Gritsenko, S. V. Mikhailenko","doi":"10.34215/1609-1175-2023-1-70-74","DOIUrl":"https://doi.org/10.34215/1609-1175-2023-1-70-74","url":null,"abstract":"Objective. To characterize pathogenic pheno- and genoprofiles of staphylococci of different species, isolated from the secretion of the prostate gland in male patients with chronic bacterial prostatitis.Materials and methods. The bacterial spectrum of microflora was studied by a bacteriological method; the species identification of microorganisms was carried out by mass spectrometry. Detection of genes determining pathogenicity factors was carried out by PCR. The biofilm-forming ability of staphylococci, as well as their anti-lysozyme, hemolytic, and adhesive activity, were determined by photometry.Results. Staphylococci were found to be dominant in the pathogen structure of chronic bacterial prostatitis. These microorganisms, regardless of their species, had a pronounced pathogenic potential. Specific features in the bioprofiles of cultures of different species were determined according to the severity of the studied biological properties. Thus, the hemolytic activity and biofilm-forming ability was significantly higher in S. aureus. Conversely, CNS strains were characterized by high anti-lysozyme activity. A significant difference was noted in the prevalence of genes that determine pathogenicity factors in the studied staphylococci of different species.Conclusion. The pathogenic bioprofile of staphylococci of various species isolated from patients with chronic bacterial prostatitis can be used as a criterion in the search and identification of the pathogen, as well as in the development of effective therapeutical approaches.","PeriodicalId":19705,"journal":{"name":"Pacific Medical Journal","volume":"16 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-04-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"79343588","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Species composition of genus Candida in healthy people and people with intestinal pathology","authors":"V. Prokopiev, Yu.V. Vinnikova, E. Peredelskaya, T. Safyanova","doi":"10.34215/1609-1175-2023-1-86-89","DOIUrl":"https://doi.org/10.34215/1609-1175-2023-1-86-89","url":null,"abstract":"Aim. To identify the difference in the detection of yeasts of the genus Candida and their species composition in people with gastrointestinal pathology and in healthy people undergoing a regular medical checkup.Materials and methods. Clinical trial material comprised biological material from patients undergoing regular medical checkup (control group) and patients with intestinal pathology. Following the detection of fungal colonies on Sabouraud medium, identification was carried out using chromogenic agar. A number of strains were identified via mass spectrometry.Results. The study results show that the frequency of occurrence of the genus Candida is more than three times higher in patients with intestinal pathology. In addition, the study groups revealed the differences in the species composition. Candida krusei is more than 5 times more common in cases of gastrointestinal pathology.Conclusion. Due to the differences in the frequency and species composition of yeasts of the genus Candida isolated from the intestine, specialists should diagnose intestinal diseases and choose antimycotic therapy in a meticulous way.","PeriodicalId":19705,"journal":{"name":"Pacific Medical Journal","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-04-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"88601063","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Analysis of quality of life indicators in medical students in the Sakhalin Oblast","authors":"V. Kuznetsov, E. Krukovich, V. B. Shumatov, L. Trankovskaya, A. A. Krukovich","doi":"10.34215/1609-1175-2023-1-94-99","DOIUrl":"https://doi.org/10.34215/1609-1175-2023-1-94-99","url":null,"abstract":"Aim. To study the indicators of quality of life (QoL) and health-related quality of life (HRQoL) among medical university students.Materials and methods. In total, 114 people aged 18 to 23 years were included in the study. The participants were divided into two groups. Group 1 consisted of 55 medical students (MS) of the Pacific State Medical University undergoing practical training in the city of Yuzhno-Sakhalinsk (Vladivostok, Russia). In this group, male and female respondents accounted for 41.8% (23) and 58.2% (32), respectively. Group 2 included 59 students of non-medical education (NMS) from the Sakhalin State University (Yuzhno-Sakhalinsk, Russia), with 49.28% (29) male and 50.8% (30) female respondents. The effect of socio-economic factors on HRQoL indicators was determined, along with the main predictors in their decrease.Results. In the structure of diseases defining the 2nd and 3A health groups, diseases of class IX prevailed. Diseases of the circulatory system (I00-I99) were detected in 12% and 24.4% of MS and NMS, respectively. The diseases of class XI were ranked second. Thus, digestive diseases (K00-K93) accounted for 47.8%, and ophthalmic diseases (H00-H59) accounted for 35.5%. A significant physical dysfunction was identified in more than 3% of respondents; 99% of respondents reported a decrease in working capacity. Among MS, 1.7% of respondents demonstrated signs of depression and suffered from pain of varying intensity (44.5%). A decreased social activity was observed in 4% of NMS respondents.Conclusion. The data of QoL monitoring can be used for developing a multi-level scoring system for preventing health disorders among university students. Proposals were made concerning healthy lifestyle management. The model of dynamic monitoring of the health of university students exposed to various risk factors was supplemented. An individual route for rehabilitation and disease prevention for university students was developed based on the study of QoL.","PeriodicalId":19705,"journal":{"name":"Pacific Medical Journal","volume":"80 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-04-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"87111669","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Wound microflora and antibiotic resistance in burn patients","authors":"A. Potapov, S. H. Shamaeva, A. Ivanova, S. V. Semenova","doi":"10.34215/1609-1175-2023-1-81-85","DOIUrl":"https://doi.org/10.34215/1609-1175-2023-1-81-85","url":null,"abstract":"Aim. To explore the etiological structure of wound microflora and its antimicrobial susceptibility in burn patients treated in hospital burn departments.Materials and methods. The authors analyzed the microbiology testing results of 2354 wound exudates in samples of 1581 patients with severe burns who underwent treatment in the Burn Unit and Department of Anesthesiology, Resuscitation and Intensive Care of the Center for Emergency Medical Care of the Republic of Sakha (Yakutia) in the period from 2016 to 2020. Data processing and antibiotic resistance analysis were performed via AMRcloud online platform (Beta version, 05.07.2022).Results. Of the pathogens isolated in the burn wounds, Staphylococcus aureus accounted for 26.1%, Enterococcus faecalis – 24.5%, Pseudomonas aeruginosa – 11.9%, Klebsiella pneumonia – 9.4%, Acinetobacter baumannii – 7.9% and Escherichia coli – 6.0%. The susceptibility of isolated microbial strains to carbapenems and high resistance to cephalosporins and fluoroquinolones were established.Conclusion. Microbial strains detected in burn patients indicate infection of wounds with representatives of nosocomial flora and their high resistance to commonly used antibiotics.","PeriodicalId":19705,"journal":{"name":"Pacific Medical Journal","volume":"35 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-04-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"80341085","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Simultaneous vegetation of opportunistic oral microorganisms in chronic periodontitis with recurrent course","authors":"N. Strelnikova, V. Tsarev, A. A. Antonova, E. S. Shapovalenko, V. E. Skorobogatova","doi":"10.34215/1609-1175-2023-1-64-69","DOIUrl":"https://doi.org/10.34215/1609-1175-2023-1-64-69","url":null,"abstract":"Aim. To evaluate clinical significance of simultaneous vegetation by conducting microbiological research of the species composition in the periodontal pockets and on the surface of the tongue root and back in chronic (generalized) periodontitis.Materials and methods. A prospective microbiological analysis of clinically significant species of opportunistic microorganisms in pathological periodontal pockets and the microbiome of the tongue root and back, in chronic (generalized) periodontitis of mild, moderate and severe degree, was carried out in 88 patients of the WHO key group, aged 35–44 (CI 95% 34.8–44.3), of the Unistom Dental Clinic of the Far Eastern State Medical University, Khabarovsk Krai, for the period 2016–2019.Results. The species composition of opportunistic microorganisms in the biotopes of the pathological periodontal pockets and tongue root and back was the same in chronic periodontitis, with a mean value of 89.7% ±2.9% (CI 95% 85.8–94.4). The mean value of the number of species of clinically significant opportunistic microorganisms in the periodontal pockets and root and back of the tongue in associations accounted for 3.9 (CI 95% 1.8–5.4). Bacteria of the genus Leptotrichia were detected simultaneously in periodontal pockets and on the root and back of the tongue in clinically significant associations with Staphylococcus aureus (CI 95% 37.3–65.4), mean Shannon index H value 3.1 ± 0.4; mean Simpson index C value 0.39 ± 0. Bacteria of the genus Leptotrichia in association with Streptococcus pyogenes (CI 95% 35.8–79.3), mean Shannon index H 2.9 ± 0.3; mean value of the Simpson index C 0.28 ± 0.06, reliably support the recurrent course of the opportunistic infectious and inflammatory process in the periodontium. The dominant role of Staphylococcus aureus and Streptococcus pyogenes in the biotopes of periodontal pockets and the root and back of the tongue in exacerbations of chronic periodontitis has been established. Titre of opportunistic microorganisms in pathological periodontal pockets of 2–5lgCFU (colony-forming units) in mild and moderate degree of severity is 1–2 order lower than titre of opportunistic microorganisms in root and back of the tongue of 4–6lgCFU (95% CI 2.1–6.5).Conclusions. Evaluation of simultaneous vegetation of opportunistic microorganisms in the oral microbiome provides a means for improving laboratory (microbiological) diagnostics in chronic periodontitis and applying a non-invasive microbiological method for indirect diagnosis of the species composition of opportunistic microorganisms in periodontal pockets according to the composition of opportunistic microorganisms of the root and back of the tongue in chronic periodontitis for prophylaxis and treatment personalization.","PeriodicalId":19705,"journal":{"name":"Pacific Medical Journal","volume":"416 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-04-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"76639190","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Evaluating nutrition, physical development and morbidity in primary school-aged children","authors":"E. Tkachuk, N. E. Globenko","doi":"10.34215/1609-1175-2023-1-90-93","DOIUrl":"https://doi.org/10.34215/1609-1175-2023-1-90-93","url":null,"abstract":"Aim. To explore the relationship of physical development and nutrition pattern in primary school-aged children.Materials and methods. 266 children have been examined for three years by means of the continuous sampling method. In order to study nutrition patterns, the questionnaire method was applied to the parents. Physical development was evaluated according to main anthropometric indicators with the identification of somatotype and degree of harmonious development. Morbidity was studied by extracting data from individual development histories.Results. The nutrition of primary schoolers is marked by a prevalence of carbohydrates, while the amount of protein and calcium-containing products is insufficient. The physical development is disharmonious in most cases, due to underweight (82% of children) and excess weight (8%). 24% of children have a postural abnormality, 15% – caries, 3% – endemic goiter, 45% suffer acute respiratory infections more than 4 times a year.Conclusions. Nutrition patterns in primary school-aged children are characterized by unbalanced diet, which contributes to disharmonious development and decreases stamina.","PeriodicalId":19705,"journal":{"name":"Pacific Medical Journal","volume":"58 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-04-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"83946530","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Virome of ancient wolf mummy (Canis lupus) as a potential source of virulent bacteriophages for phage therapy","authors":"A. E. Goncharov, Ya. A. Ahremenko, D. Azarov, M. J. Cheprasov, S. E. Fedorov, A. Solomenny, V. Kolodzhieva","doi":"10.34215/1609-1175-2023-1-35-37","DOIUrl":"https://doi.org/10.34215/1609-1175-2023-1-35-37","url":null,"abstract":"Aim. To investigate the potential for searching new virulent bacteriophages in the paleontological material extracted from the permafrost zone.Material and methods. The virome structure of the colon content of the wolf pup mummy (Canis lupus) from the late Pleistocene was evaluated by means of shotgun metagenomic sequencing.Results. The study demonstrated the predominance of Myoviridae tailed bacteriophages, including PhiKZ-like phages, in the structure of the virome.Conclusion. The results of the study indicate the possibility of using paleontological material preserved in the ancient Arctic permafrost as a resource for searching and isolating new virulent bacteriophages.","PeriodicalId":19705,"journal":{"name":"Pacific Medical Journal","volume":"29 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-04-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"81522685","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Association of gene polymorphisms of angiotensin metabolism with left ventricular diastolic dysfunction in hypertensive patients","authors":"T. V. Kalinkina, N. Lareva, M. V. Chistyakova, M. Serkin","doi":"10.34215/1609-1175-2023-1-44-49","DOIUrl":"https://doi.org/10.34215/1609-1175-2023-1-44-49","url":null,"abstract":"Aim. To investigate the relationship of SNP ACE ((AluIns/DelI>D), rs 4646994), SNP of angiotensinogen 1 gene AGT :521 C>T ((Thr 174 Met), rs 4762), SNP of angiotensinogen 2 gene AGT:704 T>C ((Met 235 Thr), rs699), SNP of angiotensin-2 type 1 receptor gene AGTR1: A1166C; A>C (rs5186) with left ventricular diastolic dysfunction in hypertensive patients.Methods and materials. 74 patients (26 women and 48 men) with stage 1-2 hypertension were examined. The analysis of genetic polymorphisms was carried out by means of polymerase chain reaction method with the analysis of DNA RFLP (restriction fragment length polymorphism).Results. CC polymorphisms of genotype AGT:704 T>C (Met 235 Thr), (χ2 = 8.18; p = 0.017) were detected in the group of patients with left ventricular diastolic dysfunction, which nearly triples the probability of impaired myocardial relaxation (OR 2.85) and CC polymorphisms of genotype of angiotensinogen type 1 receptor gene (AGTR1: A1166C), (χ2 = 1.77; p = 0.041), which doubles the probability of left ventricular diastolic dysfunction (OR 2.39).Conclusion. CC genotype AGT:704 T>C (Met 235 Thr) and CC genotype of the angiotensinogen type 1 receptor gene (AGTR1: A1166C) are associated with the development of left ventricular diastolic dysfunction in patients with hypertension.","PeriodicalId":19705,"journal":{"name":"Pacific Medical Journal","volume":"60 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-04-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"82555338","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}