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Alcohol Drinking in Adolescents Due to the COVID-19 Pandemic. 青少年因 COVID-19 大流行而饮酒。
IF 0.7 4区 农林科学 Q3 AGRICULTURE, MULTIDISCIPLINARY Pub Date : 2022-12-01 DOI: 10.5152/pcp.2022.22493
Eungyeong Kim

Background: The COVID-19 pandemic caused changes in individuals' daily lives, including drinking habits. This study attempted to identify factors of Korean adolescents' current alcohol drinking due to the COVID-19 pandemic.

Methods: The study used secondary data from the 17th Korean Youth Risk Behavior Web-based Survey that included 54 848 individuals. In this study, current alcohol drinking was defined as consuming at least 1 alcoholic drink per day for the past month. Changes in physical activity, mealtimes, smoking, and depressive symptoms were recorded.

Results: The multiple logistic regression results show that the risk for current alcohol drinking due to the COVID-19 pandemic was 1.14 times higher in increased physical activity (95% CI: 1.04-1.25, P = .007); 1.18 times higher in increased skipping breakfast (95% CI: 1.07-1.30, P = .001); 1.22 times higher in decrease skipping breakfast (95% CI: 1.10-1.36, P < .001); and 15.69 times higher in increase smoking (95% CI: 12.53-19.64, P < .001).

Conclusion: It is necessary to improve social support systems and related policies for adolescents who experienced a change in daily habits due to the COVID-19 pandemic. Our results may contribute to preparing strategies and support measures to reduce drinking by developing personalized healthcare in the future.

背景:COVID-19 大流行改变了人们的日常生活,包括饮酒习惯。本研究试图找出因 COVID-19 大流行而导致韩国青少年目前饮酒的因素:本研究使用了第 17 次韩国青少年危险行为网络调查的二手数据,调查对象包括 54 848 人。在这项研究中,目前饮酒的定义是在过去一个月中每天至少饮用 1 杯酒精饮料。研究还记录了体力活动、进餐时间、吸烟和抑郁症状的变化:多元逻辑回归结果显示,因 COVID-19 大流行而导致当前饮酒的风险在增加体育锻炼中高出 1.14 倍(95% CI:1.04-1.25,P = .007);在增加不吃早餐中高出 1.18倍(95% CI:1.07-1.30,P = .001);不吃早餐的减少率为1.22倍(95% CI:1.10-1.36,P < .001);吸烟的增加率为15.69倍(95% CI:12.53-19.64,P < .001):结论:对于因 COVID-19 大流行而改变日常习惯的青少年,有必要改善社会支持系统和相关政策。我们的研究结果可能有助于未来通过发展个性化医疗保健来制定减少饮酒的策略和支持措施。
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引用次数: 0
The sweat fatty acid content of Holstein and Jersey cows in Summer 荷斯坦奶牛和泽西奶牛夏季汗液脂肪酸含量
IF 0.8 4区 农林科学 Q3 AGRICULTURE, MULTIDISCIPLINARY Pub Date : 2022-11-01 DOI: 10.21162/pakjas/22.1534
Özgül Anitaş
Sweat is important in thermoregulation, helping to dissipate heat in the body by evaporation in hot conditions in dairy cows. The purpose of this study was to evaluate the variation of sweat fatty acid compositions in summer conditions in Jersey and Holstein dairy cattle for their potential thermoregulatory roles in animal health protection. Temperature, relative humidity and Temperature-Humidity Index (THI) values were calculated for the intensity of stress in the indoor environment. The chemical compounds of sweat were determined by gas chromatography device and inter-breeds differences were revealed. As a result of analyzes, a total of 23 fatty acids were detected in both breeds. It was understood that there were 15 fatty acids in the Jersey breed. Palmitic acid was detected at a rate of 27.72 % in Holstein cows and 25.16 % in jersey cows that may be due to a higher rate of fatty acid release from muscle tissue, so that it may be less resistant than the jersey breed in heat stress conditions. It can be said that palmitic acid can be an indicator for heat stress conditions. ∑SFA and ∑PUFA contents of the sweat of Holstein cows were lower than those of Jersey breeds while ∑MUFA content was higher than those of Jersey breeds. The high expulsion of ∑SFA and ∑PUFA in the sweat of the Jersey cows can provide the function of delaying the body's dehydration by forming a hydrolipid film on the skin surface in hot conditions
汗水在体温调节中很重要,有助于奶牛在炎热的条件下通过蒸发来散发体内的热量。本研究的目的是评估泽西和荷斯坦奶牛夏季汗液脂肪酸组成的变化,以了解其在动物健康保护中的潜在体温调节作用。计算了室内环境应力强度的温度、相对湿度和温湿度指数(THI)值。采用气相色谱法测定汗液的化学成分,揭示了品种间的差异。分析结果表明,在这两个品种中共检测到23种脂肪酸。据了解,泽西品种有15种脂肪酸。在荷斯坦奶牛和泽西奶牛中,棕榈酸的检测率分别为27.72%和25.16%,这可能是由于肌肉组织中脂肪酸释放率较高,因此在热应激条件下,它的抵抗力可能不如泽西奶牛。可以说,棕榈酸可以作为热应激条件的指示物。荷斯坦奶牛汗液中∑SFA和∑PUFA含量低于泽西品种,∑MUFA含量高于泽西品种。在高温条件下,泽西奶牛汗液中大量排出的∑SFA和∑PUFA可在皮肤表面形成一层水脂膜,起到延缓机体脱水的作用
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引用次数: 0
Drought tolerance enhancment in cotton (Gossypium hirsutum L.) by mepiquate chloride seed priming 氯化物浸种提高棉花(棉)耐旱性
IF 0.8 4区 农林科学 Q3 AGRICULTURE, MULTIDISCIPLINARY Pub Date : 2022-11-01 DOI: 10.21162/pakjas/22.1147
Gafouri Shahrbano
To study pre-sowing seed treatment (seed priming) of hormone Mepiquat chloride (MC) (PIX) on physiological properties and increase drought tolerance in cotton (Shayan cultivar), a factorial experiment was performed in a completely randomized design with 3 replications. In this research, the seeds were initially exposed to two doses of the growth-regulatory hormone mepiquat chloride, including 5 and 10 g/l and control before being planted in pots.After the emergence of the plant from the soil, dry treatments based on the soil field capacity (FC) at three levels of irrigation at 25, 50, and 100% of field capacity for six weeks are applied to them, and growth indices, the activity of antioxidant enzymes, amount of hydrogen peroxide and malondialdehyde as well as changes in the two hormones of abscisic acid and endogenous gibberellin was investigated. According to the results of this research, the effect of seed priming with PIX and the interaction of drought × pre-sowing seed treatment on all physiological traits were significant at the level of 1%. The results of experimental data showed that drought stress (especially severe drought) reduced most growth indicator and physiological traits (fresh and dry weight of root and shoot, shoot length, fresh and dry weight of leaf, leaf area, root peroxidation activity, leaf phenol content, amount of gibberellin ) and only increased abscisic acid content (74%), root diameter (38%), root length(14%), leaf thickness, (15%) compared to the control. pre-sowing seed treatment with PIX, while increasing the dry weight (28% roots and 34% leaves) and thickness roots (58%) and 96% leaves (96%), increased catalase (88%) and peroxidase (6%) activity and abscisic acid content (73%) of leaves, reduced hydrogen peroxide (17%), malondialdehyde (20%) of leaves, and gibberellin (40%) of roots. Under extreme drought stress, the application of PIX decreased the amount of phenolic compounds in leaves by 44% as compared to the control. The interplay of drought stress × PIX decreased root gibberellin by 25% and raised leaf abscisic acid by 183%. According to the study results, seed priming with PIX improved the physiological and biochemical responses of cotton under drought conditions
采用完全随机设计、3个重复的因子试验,研究播前种子处理(PIX)对沙燕棉花生理特性和抗旱性的影响。在这项研究中,在盆栽之前,种子最初暴露于两种剂量的生长调节激素氯化物,包括5和10克/升和对照。植株出苗后,按土壤田容量(FC)、25%、50%和100%三种灌溉水平分别进行6周的干处理,观察植株生长指标、抗氧化酶活性、过氧化氢和丙二醛含量以及脱落酸和内源赤霉素两种激素的变化。本研究结果表明,在1%水平下,PIX催种和干旱×播前处理互作对各生理性状的影响均显著。结果表明,与对照相比,干旱胁迫(特别是严重干旱)降低了大部分生长指标和生理性状(根冠鲜干重、茎长、叶片鲜干重、叶面积、根过氧化活性、叶酚含量、赤霉素含量),仅增加了脱落酸含量(74%)、根径(38%)、根长(14%)、叶厚(15%)。播前PIX处理增加了干重(28%根和34%叶)、根厚(58%)和叶厚(96%),提高了过氧化氢酶(88%)、过氧化物酶(6%)活性和脱落酸含量(73%),降低了过氧化氢(17%)、丙二醛(20%)和赤霉素(40%)含量。在极端干旱胁迫下,与对照相比,施用PIX使叶片中酚类化合物的数量减少了44%。干旱胁迫与PIX的交互作用使根系赤霉素减少25%,叶片脱落酸增加183%。结果表明,PIX灌种可改善棉花在干旱条件下的生理生化反应
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引用次数: 0
Eco-physiological and morphological responses of Pinellia ternate from different sources under drought stress 不同来源三叶半夏在干旱胁迫下的生态生理和形态响应
IF 0.8 4区 农林科学 Q3 AGRICULTURE, MULTIDISCIPLINARY Pub Date : 2022-11-01 DOI: 10.21162/pakjas/22.132
Zhao Na
The objective of the current study was to investigate the effect of drought stress on yield, physiological and biochemical indexes and changes of ecological parameters of artificially cultivated Pinellia ternata. Pinellia ternata were taken from four regions including Changde, Fuling, Ziyang and Bijie. A 75%, 65%, and 55% field water capacity processing were set under the same planting conditions and changes in morphology, photosynthesis, antioxidant enzyme activity and final yield of Pinellia ternata during the 80-day growth period was determined. Results of current study explored that when the field water capacity drops from 75% to 55%, the leaf area of Bijie Pinellia ternate decreased from 15.29 cm2 to 10.88 cm2 while Ziyang Pinellia ternate tuber fresh weight decreased from 2.58 g to 1.48 g. The content of chlorophyll a in Bijie Pinellia ternate decreased from 1.21 mg/g to 0.89 mg/g . Moderate and severe drought stress can significantly reduce the content of chlorophyll and soluble protein in leaves of Pinellia ternate, while CAT activity, SOD activity and POD activity showed different degrees of increase. Pinellia ternate of different regions showed certain interspecies differences in terms of changes in leaf area, fresh root yield, chlorophyll b content, MDA content, and CAT activity. Based on results, it is concluded that moderate drought stress can cause Pinellia ternate to produce significant ecological and physiological changes, and also affecting its tuber yield. 75% of field water holding capacity is a suitable soil moisture condition
本研究旨在探讨干旱胁迫对人工栽培的菠萝产量、生理生化指标及生态参数变化的影响。从常德、涪陵、资阳、毕节等四个地区采集到了山药材。在相同的种植条件下,分别进行75%、65%和55%的田间持水量处理,测定了80天生长期内菠萝形态、光合作用、抗氧化酶活性和最终产量的变化。研究结果表明,当田间持水量从75%下降到55%时,毕节的叶面积从15.29平方厘米下降到10.88平方厘米,紫阳的鲜重从2.58克下降到1.48克。毕节的叶绿素a含量从1.21毫克/克下降到0.89毫克/克。中度和重度干旱胁迫可显著降低菠萝叶片中叶绿素和可溶性蛋白的含量,而CAT活性、SOD活性和POD活性则呈现不同程度的升高。不同地区的菠萝在叶面积、鲜根产量、叶绿素b含量、MDA含量和CAT活性等方面表现出一定的种间差异。研究结果表明,中度干旱胁迫可使菠萝产生显著的生态和生理变化,并影响其块茎产量。75%的田间持水量是合适的土壤水分条件
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引用次数: 0
Detection of watermelon in RGB images via unmanned aerial vehicle by utilising texture features for predicting yield 利用纹理特征预测产量的RGB西瓜无人机检测
IF 0.8 4区 农林科学 Q3 AGRICULTURE, MULTIDISCIPLINARY Pub Date : 2022-11-01 DOI: 10.21162/pakjas/22.873
A. Ekiz
Unmanned aerial vehicles equipped with a digital camera are one of technologies that the precision agriculture takes advantage. In this study conducted in 2020, watermelons in the images obtained by an unmanned aerial vehicle from the watermelon field, which is in Sarıçam, Adana, Turkey, were segmented. The original image was processed in two ways. First, the image was converted to grayscale and then divided into blocks by an overlapping sliding window. The grey level co-occurrence matrixes from these blocks were obtained and six Haralick texture features were computed. Then, blocks were classified to one of three categories, soil, leaf and watermelon, by employing a multiclass support vector machine with radial basis kernel. Meanwhile, the original image was partitioned into three groups by using k-means clustering and the group having the highest blue component at its centre was selected. Finally, the two outcomes were fused to obtain possible watermelon regions in the image. The average categorization accuracy and the rate of detected watermelons without incorporating clustering outcome were 96.51% and 98.50% respectively. The watermelon detection performance was enhanced by fusing the clustering result
配备数码相机的无人机是精准农业所利用的技术之一。在2020年进行的这项研究中,无人机从土耳其阿达纳Sarıçam的西瓜地获得的图像中的西瓜被分割。原始图像有两种处理方式。首先,将图像转换为灰度,然后通过重叠的滑动窗口将其划分为块。从这些块中获得灰度共生矩阵,并计算出六个Haralick纹理特征。然后,通过使用具有径向基核的多类支持向量机,将块划分为土壤、叶子和西瓜三类之一。同时,通过使用k均值聚类将原始图像划分为三组,并选择在其中心具有最高蓝色分量的组。最后,将这两个结果进行融合,以获得图像中可能的西瓜区域。平均分类准确率和未纳入聚类结果的西瓜检出率分别为96.51%和98.50%。融合聚类结果提高了西瓜的检测性能
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引用次数: 0
Determination of Adenosine phosphate in beef by QuEChERS- Graphene -UPLC QuEChERS-石墨烯-UPLC法测定牛肉中磷酸腺苷
IF 0.8 4区 农林科学 Q3 AGRICULTURE, MULTIDISCIPLINARY Pub Date : 2022-11-01 DOI: 10.21162/pakjas/22.140
Xiaoyan Duan
A rapid and highly sensitive modified QuEChERS method was combined with ultrahigh performance liquid chromatography (UPLC) to determine the content of adenosine phosphate in beef sample. In view of the complexity of the beef sample matrix, the cold perchloric acid was used as the solvent combined with vortex oscillation were selected to extracted the adenosine phosphate in the beef sample. 10mg graphene was used to remove interferences such as fat and pigment in beef, and UPLC was used for quantitative analysis. The three adenosine phosphates in beef showed a good linear relationship in the concentration range of 0.5-20 μg/mL, the linear correlation coefficient R2>0.99, and the average recovery rate of the three adenosine phosphate addition levels in beef samples was between 85.25%-100.12%, and the RSD was ranged from 1.63% to 3.69%. The adenosine phosphate content in commercially available beef samples further verifies the feasibility of this method. The proposed method has the advantages of simple and fast operation, good purification effect, high sensitivity and accuracy, therefor, may provide a new method for efficient and accurate evaluation of the quality and safety of beef
建立了一种快速、高灵敏度的改良QuEChERS法与超高效液相色谱法(UPLC)相结合的方法测定牛肉样品中磷酸腺苷的含量。针对牛肉样品基质的复杂性,选择冷高氯酸作为溶剂,结合涡旋振荡提取牛肉样品中的磷酸腺苷。用10mg石墨烯去除牛肉中的脂肪、色素等干扰物,用UPLC进行定量分析。牛肉中3种磷酸腺苷在0.5 ~ 20 μg/mL浓度范围内呈良好的线性关系,线性相关系数R2>0.99,牛肉样品中3种磷酸腺苷添加水平的平均回收率为85.25% ~ 100.12%,RSD为1.63% ~ 3.69%。市售牛肉样品中磷酸腺苷的含量进一步验证了该方法的可行性。该方法操作简单快捷,纯化效果好,灵敏度和准确度高,可为高效准确评价牛肉质量安全提供一种新方法
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引用次数: 0
Development of new two methods based on low-cost sensors for determination of calcium carbonate contents of soils 基于低成本传感器的两种新的土壤碳酸钙含量测定方法的发展
IF 0.8 4区 农林科学 Q3 AGRICULTURE, MULTIDISCIPLINARY Pub Date : 2022-11-01 DOI: 10.21162/pakjas/22.148
H. Karadöl
The chemical analysis of CaCO3 contents of soils provide information about not only efficiency of soil for supplying plant with nutrients but also identification of factors affecting this efficiency in the soil. The aim of the current experiment was to develop new methods based on sensors and compare with conventional Scheibler method. The CO2 liberated by the action of HCl on CaCO3 content of the soil in a reaction bottle and an accumulation chamber was determined by pressure sensor and CO2 sensor, respectively. The CaCO3 contents of soils were estimated using the regression equation of standard curves. The CaCO3 contents of soils obtained using Scheibler calcimetric, pressure and CO2 sensor methods ranged from 0.20 to 54.74%, 0.25 to 54.10% and 0.55 to 53.05%, respectively. On the basis of linearity of calibration curve and high correlation with Scheibler calcimetric method it can be said that developed pressure and CO2 sensor methods appears to be very useful for quantification of CaCO3 contents of soils. The pressure sensor has provided the opportunity in developing a simple and handy device with an affordable cost to measure CaCO3 contents of soils as accurately as Scheibler calcimeter when compared with CO2 sensor method. Even if there is a good relationship between Scheibler calcimetric method and CO2 sensor method the cost of CO2 sensor method may limit the use in determination of CaCO3 contents of soils. However, CO2 sensor method can be used to monitor CO2 evolved in biochemical process such as respiration and fermentation
土壤中CaCO3含量的化学分析不仅提供了有关土壤向植物提供养分的效率的信息,而且还确定了影响土壤中这种效率的因素。当前实验的目的是开发基于传感器的新方法,并与传统的Scheibler方法进行比较。通过压力传感器和CO2传感器分别测定HCl对反应瓶和积聚室中土壤的CaCO3含量的作用释放的CO2。采用标准曲线回归方程估算了土壤中CaCO3的含量。使用Scheibler钙化法、压力法和CO2传感器法获得的土壤CaCO3含量分别为0.20至54.74%、0.25至54.10%和0.55至53.05%。基于校准曲线的线性和与Scheibler钙化法的高度相关性,可以说,开发的压力和CO2传感器方法似乎对土壤CaCO3含量的定量非常有用。与CO2传感器方法相比,压力传感器为开发一种简单方便、成本合理的设备提供了机会,可以像Scheibler钙化仪一样准确地测量土壤中的CaCO3含量。即使Scheibler钙化法和CO2传感器法之间存在良好的关系,CO2传感器法的成本也可能限制在土壤CaCO3含量测定中的使用。然而,CO2传感器方法可以用于监测呼吸和发酵等生化过程中产生的CO2
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引用次数: 0
An integrated fuzzy-rough set model for identification of tea leaf diseases 茶叶病害识别的模糊-粗糙集综合模型
IF 0.8 4区 农林科学 Q3 AGRICULTURE, MULTIDISCIPLINARY Pub Date : 2022-11-01 DOI: 10.21162/pakjas/22.1403
J. S. Krishnan
Tea is one of the major economic crops of India. People use tiny tea leaves to make beverages. The diseases in tea leaves affect the quality and yield of this cultivation. This paper proposes a disease classification model to prevent the major loss in crop yield.Tea leaf images are captured using a camera, and various image processing techniques are applied to the images to identify which disease is affected. The proposed model works for three major tea leaf diseases: blister blight, scab, and spot. The model extracts the Haralick features using Gray Level Co-occurrence Matrix (GLCM), and the most relevant features are selected with the help of the metaheuristic optimization technique. Fuzzy Rough Nearest Neighbor (FRNN) is used for classification techniques, and the model gave better accuracy than other existing techniques
茶是印度的主要经济作物之一。人们用微小的茶叶制作饮料。茶叶中的病害影响了茶叶的品质和产量。本文提出了一种防止作物产量重大损失的病害分类模型。使用相机拍摄茶叶图像,并将各种图像处理技术应用于图像,以识别哪些疾病受到影响。该模型适用于三种主要的茶叶病害:水泡病、赤霉病和斑点病。该模型使用灰度共生矩阵(GLCM)提取Haralick特征,并借助元启发式优化技术选择最相关的特征。模糊粗糙最近邻(FRNN)用于分类技术,该模型比现有的其他技术具有更好的精度
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引用次数: 1
Morphological, physiological, and biochemical response of citrus rootstocks to salt stress 柑桔砧木对盐胁迫的形态、生理和生化反应
IF 0.8 4区 农林科学 Q3 AGRICULTURE, MULTIDISCIPLINARY Pub Date : 2022-11-01 DOI: 10.21162/pakjas/22.111
Şeyma Arıkan
Soil salinity is one of the most significant stressors limiting plant productivity in the world's irrigated and cultivated agricultural lands. Plants have a wide range of adaptations and mitigation strategies to overcome salinity effects. It is possible to mitigate the effects of salt stress by the development of resistant cultivars and more efficient use of available resources. However, these methods are time-consuming and costly. Solutions to salinity issues need to be developed that are both straightforward and inexpensive, as they will likely only be implemented temporarily. In this study, the effects of the different salinity levels (Control, 20mM, 40mM, 60mM, 80mM, and 100mM) on citrus rootstocks were determined. In the citrus growing regions, the sour orange, trifoliate orange, and Carrizo citrange were the three most popular rootstocks. As a result of the present study, the sour orange was found to have the lowest decline in plant height (13.36%), leaf area (31.19%), and root length (8.13%), while the trifoliate orange had the lowest decrease in fresh weight of the plant (6.82%) and root (4.42%). Trifoliate orange was shown to have the highest levels of catalase activity, whereas superoxide dismutase and peroxidase activities were found to be highest in Carrizo citrange and sour orange, respectively. The highest concentration of plant nutrients was found in the Carrizo citrange. Therefore, sour orange was discovered to be more salinity tolerant in terms of morphological traits, physiological qualities, biochemical composition, and plant nutrition than trifoliate orange and Carrizo citrange. For areas with high salt levels, growing citrus on sour orange rootstock may be a viable alternative
在世界灌溉和耕地中,土壤盐度是限制植物生产力的最重要的压力源之一。植物有广泛的适应和缓解策略来克服盐度的影响。通过培育抗性品种和更有效地利用可用资源,可以减轻盐胁迫的影响。然而,这些方法耗时且成本高昂。需要制定既简单又廉价的盐度问题解决方案,因为这些解决方案可能只是暂时实施的。在本研究中,测定了不同盐度水平(对照、20mM、40mM、60mM、80mM和100mM)对柑橘砧木的影响。在柑橘产区,酸橙、三叶橙和香橙是最受欢迎的三种砧木。本研究结果表明,酸橙的株高(13.36%)、叶面积(31.19%)和根长(8.13%)下降幅度最小,而三叶橙的植株鲜重(6.82%)和根重(4.42%)下降幅度最低,而超氧化物歧化酶和过氧化物酶活性在Carrizo citrange和酸橙中分别最高。香橙中的植物营养成分浓度最高。因此,酸橙在形态特征、生理品质、生化成分和植物营养方面比三叶橙和香橙更耐盐。对于高盐地区,在酸橙砧木上种植柑橘可能是一种可行的选择
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引用次数: 0
Transcriptomic analysis of differentially expressed genes between uterus and ovary in Xiangxi cattle 湘西牛子宫和卵巢差异表达基因的转录组学分析
IF 0.8 4区 农林科学 Q3 AGRICULTURE, MULTIDISCIPLINARY Pub Date : 2022-10-01 DOI: 10.21162/pakjas/21.657
Wu Song, Jianbo Li, Ao Sun, Y. Long
Reproductive efficiency of a heifer is directly dependent on the health of reproductive system especially ovary and uterus. Current experiment was carried out to screen candidate genes related to reproductive traits and detect molecular mechanisms of DEGs by using RNA-sequencing (RNAseq) and Gene Set Enrichment Analysis (GSEA) to understand the environmental adaptability, molecular breeding and analyze the reproductive performance. Furthermore, Illumina II high throughput sequncing technology was utilized in the current experiment to sequence transcriptomes and analysis related to pathway of different genes between uterus and ovary in Xiangxi cattle. The total number of ovary assembled was compared to the reference genome sequence, DEGs analysis, GO and KEGG enrichment analysis and candidate gene screening were performed. Based on the transcriptome data, seven cDNA libraries were constructed by utilizing total RNA. Out of seven contracted cDNA libraries, in every sequencing library, raw reads reached >133 Mb, and >94% clean reads remained. After comparing the Xiangxi cattle ovaries with the uterus, a total of 3635 mRNAs were found, in which 1608 were significantly up-regulated expressed and 2027 were down-regulated expression mRNAs. The mRNA expression of ADCY3, ADCY4, ADCY9, FSHR, BMP15, LHCGR, INSR, CYP11A1, CYP17A1, CYP19A1, STAR and SCARB1 in ovary of Xiangxi Cattle were significant different in ovarian and uterus tissues. Through GSEA and PPI network, we found the ovarian steroidogenesis pathway played significant role in signal regulation during the development of the ovarian tissue of the Xiangxi cattle
小母牛的繁殖效率直接取决于生殖系统的健康,尤其是卵巢和子宫的健康。本实验采用RNA测序(RNAseq)和基因集富集分析(GSEA)技术,筛选与生殖性状相关的候选基因,检测DEG的分子机制,了解环境适应性、分子育种和生殖性能分析。此外,本实验还利用Illumina II高通量测序技术对湘西牛子宫和卵巢之间的转录组进行了测序,并对不同基因的通路进行了分析。将组装的卵巢总数与参考基因组序列进行比较,进行DEGs分析、GO和KEGG富集分析以及候选基因筛选。基于转录组数据,利用总RNA构建了7个cDNA文库。在七个签约的cDNA文库中,在每个测序文库中,原始读数达到>133Mb,并且保留了>94%的干净读数。将湘西牛卵巢与子宫进行比较,共发现3635个mRNA,其中1608个表达显著上调,2027个表达下调。ADCY3、ADCY4、ADCY9、FSHR、BMP15、LHCGR、INSR、CYP11A1、CYP17A1、CYP19A1、STAR和SCARB1在湘西牛卵巢和子宫组织中的mRNA表达存在显著差异。通过GSEA和PPI网络,我们发现卵巢类固醇生成途径在湘西牛卵巢组织发育过程中起着重要的信号调节作用
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引用次数: 0
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Pakistan Journal of Agricultural Sciences
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