S. Annas, M. Zamri-Saad, Md Yasin Ina-Salwany, M. Amal
Streptococcus agalactiae and Streptococcus iniae are the two main pathogens causing streptococcosis in fish. This study compares the clinicopathological changes in red hybrid tilapia experimentally infected with S. agalactiae or S. iniae. A total of 180 tilapias were divided into six groups. Groups 1A, 2A, and 3A were inoculated intraperitoneally with sterile phosphate-buffered saline, S. agalactiae, and S. iniae. Fish of Groups 1A, 2A, and 3A were then immediately allowed to cohabitate with fish of Groups 1B, 2B, and 3B, respectively. All fish were observed at 6-hr intervals for 120 hr before surviving fish were euthanized. The spleen, liver, and brain samples were collected for bacterial isolation and histopathology. Clinical signs were developed at 72 hr in Groups 2A and 3A and 96 hr in Groups 2B and 3B. Group 2A showed the highest clinical score (P<0.05). Significantly (P<0.05), more cohabitating fish (Groups 2B) were infected by S. agalactiae compared to S. iniae (Group 3B) at 55.0±0.0 and 43.70±1.25%, respectively. The mortality rate was significantly (P<0.05) higher for Groups 2A and 2B than other groups. The gross lesions were significantly (P<0.05) more common in fish of Group 2A. Histopathologically, encephalitis was observed in fish infected with S. iniae of Groups 3A and 3B, while meningoencephalitis was observed in fish infected with S. agalactiae of Groups 2A and 2B. The findings suggest that S. agalactiae is more pathogenic than S. iniae, producing slightly different histopathological lesions in the brain.
{"title":"Comparative Clinicopathological Changes Associated with Experimental Streptococcus agalactiae and Streptococcus iniae Cohabitation Infection in Red Hybrid Tilapia (Oreochromis niloticus × Oreochromis mossambicus)","authors":"S. Annas, M. Zamri-Saad, Md Yasin Ina-Salwany, M. Amal","doi":"10.47836/pjtas.46.3.10","DOIUrl":"https://doi.org/10.47836/pjtas.46.3.10","url":null,"abstract":"Streptococcus agalactiae and Streptococcus iniae are the two main pathogens causing streptococcosis in fish. This study compares the clinicopathological changes in red hybrid tilapia experimentally infected with S. agalactiae or S. iniae. A total of 180 tilapias were divided into six groups. Groups 1A, 2A, and 3A were inoculated intraperitoneally with sterile phosphate-buffered saline, S. agalactiae, and S. iniae. Fish of Groups 1A, 2A, and 3A were then immediately allowed to cohabitate with fish of Groups 1B, 2B, and 3B, respectively. All fish were observed at 6-hr intervals for 120 hr before surviving fish were euthanized. The spleen, liver, and brain samples were collected for bacterial isolation and histopathology. Clinical signs were developed at 72 hr in Groups 2A and 3A and 96 hr in Groups 2B and 3B. Group 2A showed the highest clinical score (P<0.05). Significantly (P<0.05), more cohabitating fish (Groups 2B) were infected by S. agalactiae compared to S. iniae (Group 3B) at 55.0±0.0 and 43.70±1.25%, respectively. The mortality rate was significantly (P<0.05) higher for Groups 2A and 2B than other groups. The gross lesions were significantly (P<0.05) more common in fish of Group 2A. Histopathologically, encephalitis was observed in fish infected with S. iniae of Groups 3A and 3B, while meningoencephalitis was observed in fish infected with S. agalactiae of Groups 2A and 2B. The findings suggest that S. agalactiae is more pathogenic than S. iniae, producing slightly different histopathological lesions in the brain.","PeriodicalId":19890,"journal":{"name":"Pertanika Journal of Tropical Agricultural Science","volume":" ","pages":""},"PeriodicalIF":0.6,"publicationDate":"2023-08-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"47333347","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Jeffi Christophe, Chin Ping Tan, Helmi Wasoh, Oi Ming Lai
Inca Inchi oil, an edible oil with high amounts of polyunsaturated fatty acids such as omega 3 and omega 6 fatty acids, has a wide range of applications in therapeutic, food, and pharmaceutical industries. Increasing its oil yield during oil extraction is important due to its high value. However, conventional techniques such as screw press extraction pose a limitation in terms of oil yield. Thus, in this study, the seeds were pre-treated in a microwave and hot air oven prior to oil extraction. The effects of this pre-treatment on the oil yield, fatty acid profile, antioxidant profile, and physicochemical properties were compared. Microwave treatment (4 min) was found to have the highest oil yield (43.39%) compared to control (37.76%). The proximate analysis revealed that the protein content in the oil meal was high (51–60%) compared to oil seed (24.2%), indicating that it has potential application to be developed into plant-based protein foods. The fatty acid profile indicates that the oil had high omega 3 (49%) and omega 6 (37%) fatty acids. The free fatty acids and peroxide values of the pre-treated oil samples were less than 1% and 10 meq O2/kg oil, respectively, compared to the control (1%), while the iodine value was high due to double bonds. The 2,2-diphenyl-1-1picrylhydrazyl and 2,2´-azino-bis 3-ethylbenzothiazoline-6-sulfonic acid study shows that the oil has good radical scavenging activity (70 and 90%), which shows the oil’s potential in functional food applications.
{"title":"Comparative Study of Thermal Pre-treatment on the Extraction, Antioxidant, Fatty Acid Profile, and Physicochemical Properties of Inca Inchi Seed Oil","authors":"Jeffi Christophe, Chin Ping Tan, Helmi Wasoh, Oi Ming Lai","doi":"10.47836/pjtas.46.3.11","DOIUrl":"https://doi.org/10.47836/pjtas.46.3.11","url":null,"abstract":"Inca Inchi oil, an edible oil with high amounts of polyunsaturated fatty acids such as omega 3 and omega 6 fatty acids, has a wide range of applications in therapeutic, food, and pharmaceutical industries. Increasing its oil yield during oil extraction is important due to its high value. However, conventional techniques such as screw press extraction pose a limitation in terms of oil yield. Thus, in this study, the seeds were pre-treated in a microwave and hot air oven prior to oil extraction. The effects of this pre-treatment on the oil yield, fatty acid profile, antioxidant profile, and physicochemical properties were compared. Microwave treatment (4 min) was found to have the highest oil yield (43.39%) compared to control (37.76%). The proximate analysis revealed that the protein content in the oil meal was high (51–60%) compared to oil seed (24.2%), indicating that it has potential application to be developed into plant-based protein foods. The fatty acid profile indicates that the oil had high omega 3 (49%) and omega 6 (37%) fatty acids. The free fatty acids and peroxide values of the pre-treated oil samples were less than 1% and 10 meq O2/kg oil, respectively, compared to the control (1%), while the iodine value was high due to double bonds. The 2,2-diphenyl-1-1picrylhydrazyl and 2,2´-azino-bis 3-ethylbenzothiazoline-6-sulfonic acid study shows that the oil has good radical scavenging activity (70 and 90%), which shows the oil’s potential in functional food applications.","PeriodicalId":19890,"journal":{"name":"Pertanika Journal of Tropical Agricultural Science","volume":" ","pages":""},"PeriodicalIF":0.6,"publicationDate":"2023-08-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"45420896","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Edible bird’s nest (EBN) hydrolysate is widely used in EBN downstream products. This study aimed to optimize the process conditions (combination of heat treatment and enzymatic hydrolysis) to produce high-yield and high-quality EBN hydrolysate. The effects of four factors in the process were studied by response surface methodology. The experimental factors are EBN temperature during double boiling (DB), DB duration, enzymatic hydrolysis duration, and the ratio of EBN to water. The recovery (yield) and quality (sialic acid [SA], 2,2-azino-bis-3-ethylbenzothiazoline-6-sulphonic acid [ABTS], and 2,2-diphenyl-1-picrylhydrazyl [DPPH]) of the final product were used as response variables. The Pearson correlation coefficient showed that: EBN temperature during DB affected product recovery (p < 0.01) and ABTS (p < 0.01), DB Duration affected DPPH (p < 0.01), and the ratio of EBN to water affected product recovery (p < 0.01). The duration of enzymatic hydrolysis was not significantly correlated with any of the responses and least significant factors in the model. Two optimal conditions for the processes obtained from this study were yield (product recovery) and quality. This study also showed that EBN hydrolysate produced from EBN by-products could be used as a nutraceutical because of the antioxidant activity and high SA content.
{"title":"Optimization of Process Conditions for the Production of High-yield and High-quality Edible Bird’s Nest (EBN) Hydrolysate","authors":"B. Yeo, S. Wong, C. Tan, Y. Rukayadi, O. Lai","doi":"10.47836/pjtas.46.3.12","DOIUrl":"https://doi.org/10.47836/pjtas.46.3.12","url":null,"abstract":"Edible bird’s nest (EBN) hydrolysate is widely used in EBN downstream products. This study aimed to optimize the process conditions (combination of heat treatment and enzymatic hydrolysis) to produce high-yield and high-quality EBN hydrolysate. The effects of four factors in the process were studied by response surface methodology. The experimental factors are EBN temperature during double boiling (DB), DB duration, enzymatic hydrolysis duration, and the ratio of EBN to water. The recovery (yield) and quality (sialic acid [SA], 2,2-azino-bis-3-ethylbenzothiazoline-6-sulphonic acid [ABTS], and 2,2-diphenyl-1-picrylhydrazyl [DPPH]) of the final product were used as response variables. The Pearson correlation coefficient showed that: EBN temperature during DB affected product recovery (p < 0.01) and ABTS (p < 0.01), DB Duration affected DPPH (p < 0.01), and the ratio of EBN to water affected product recovery (p < 0.01). The duration of enzymatic hydrolysis was not significantly correlated with any of the responses and least significant factors in the model. Two optimal conditions for the processes obtained from this study were yield (product recovery) and quality. This study also showed that EBN hydrolysate produced from EBN by-products could be used as a nutraceutical because of the antioxidant activity and high SA content.","PeriodicalId":19890,"journal":{"name":"Pertanika Journal of Tropical Agricultural Science","volume":" ","pages":""},"PeriodicalIF":0.6,"publicationDate":"2023-08-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"42253401","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Iffatul Arifah Yusup, Martini Mohammad Yusoff, M. Ismail, Z. Berahim, Fariz Adzmi
During low rainfall periods, rice plants often face drought stress, which would significantly affect rice yield. One of the methods to mitigate the problem is incorporating rice plants with fungi such as Trichoderma. This study evaluated the effects of encapsulated Trichoderma asperellum (UPM 40) on the growth and yield of rice plants planted in saturated and flooded soil conditions in response to drought stress. A randomized complete block factorial design was implemented with four replications and two factors. The first factor was encapsulated T. asperellum (UPM 40) concentration of 0 and 5 g. The second factor was the soil condition: saturated and flooded soil. The drought stress was imposed by halting watering during early anthesis for 14 days and resumed afterward. One of the significant interaction effects detected was on the relative water content of rice plants planted in flooded soil conditions and treated with T. asperellum (UPM 40), where the value was 78.51%, higher than the control of 72.09%, which showed the ability of the fungus to help rice plants alleviate detrimental effects of drought stress and delay the onset of adverse effects of drought stress. Thus, it contributed to the crop’s simultaneous improvement in rice yield compared to untreated plants in saturated soil. Applying 5 g encapsulated T. asperellum (UPM 40) to the rice plants would perform best in flooded soil conditions during drought stress.
{"title":"Growth and Yield Comparison of Rice Plants Treated with Encapsulated Trichoderma asperellum (UPM 40) in Response to Drought Stress","authors":"Iffatul Arifah Yusup, Martini Mohammad Yusoff, M. Ismail, Z. Berahim, Fariz Adzmi","doi":"10.47836/pjtas.46.3.09","DOIUrl":"https://doi.org/10.47836/pjtas.46.3.09","url":null,"abstract":"During low rainfall periods, rice plants often face drought stress, which would significantly affect rice yield. One of the methods to mitigate the problem is incorporating rice plants with fungi such as Trichoderma. This study evaluated the effects of encapsulated Trichoderma asperellum (UPM 40) on the growth and yield of rice plants planted in saturated and flooded soil conditions in response to drought stress. A randomized complete block factorial design was implemented with four replications and two factors. The first factor was encapsulated T. asperellum (UPM 40) concentration of 0 and 5 g. The second factor was the soil condition: saturated and flooded soil. The drought stress was imposed by halting watering during early anthesis for 14 days and resumed afterward. One of the significant interaction effects detected was on the relative water content of rice plants planted in flooded soil conditions and treated with T. asperellum (UPM 40), where the value was 78.51%, higher than the control of 72.09%, which showed the ability of the fungus to help rice plants alleviate detrimental effects of drought stress and delay the onset of adverse effects of drought stress. Thus, it contributed to the crop’s simultaneous improvement in rice yield compared to untreated plants in saturated soil. Applying 5 g encapsulated T. asperellum (UPM 40) to the rice plants would perform best in flooded soil conditions during drought stress.","PeriodicalId":19890,"journal":{"name":"Pertanika Journal of Tropical Agricultural Science","volume":" ","pages":""},"PeriodicalIF":0.6,"publicationDate":"2023-07-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"48919616","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Noor Aimi Shazana Mohd Yusoff, Fisal Ahmad, A. Zamri, S. B. Abdul Razak, Muhammad Fauzi Mahmud, Tuan Zainazor Tuan Chilek
In Malaysia, stingless bees can be categorised into two genera: Melipona and Trigona, known as “kelulut”. The high demand for kelulut honey boosts the production of the honey industry. Previous studies reported that stingless bee (kelulut) honey and its products were contaminated with pathogenic bacteria during harvesting and processing. This research aims to isolate and identify the pathogenic bacteria in kelulut honey. Forty-eight samples of kelulut honey (open and closed pot) and propolis were obtained from selected farms in Terengganu by focusing on a major stingless bee species available in Malaysia, Heterotrigona itama. In addition, the swabbing technique was done on the wooden beehive of the kelulut to evaluate the environmental contamination. The pathogenic bacteria were isolated using specifically selected agar, such as Bacillus cereus agar (for B. cereus), Baird-Parker agar (for Staphylococcus aureus), and MacConkey agar (for other pathogenic bacteria), which were confirmed through a biochemical test. All samples were analysed, and the results showed that B. cereus (7/48), Pseudomonas aeruginosa (10/48), Pantoea spp. (11/48), Serratia plymuthica (6/48), and S. aureus (9/48) were obtained in the samples. This study indicates that kelulut honey was contaminated with B. cereus, P. aeruginosa, Pantoea spp., S. plymuthica, and S. aureus. Isolated pathogenic bacteria may exist in the kelulut honey through food handlers, utensils, and the environment. Hence, the stakeholders should strictly follow good standard operating procedures and guidelines by the kelulut honey industry to prevent foodborne illness.
{"title":"Pathogenic Bacterial Communities Isolated and Identified in Stingless Bee (Kelulut) Honey from Selected Farms in Terengganu","authors":"Noor Aimi Shazana Mohd Yusoff, Fisal Ahmad, A. Zamri, S. B. Abdul Razak, Muhammad Fauzi Mahmud, Tuan Zainazor Tuan Chilek","doi":"10.47836/pjtas.46.3.08","DOIUrl":"https://doi.org/10.47836/pjtas.46.3.08","url":null,"abstract":"In Malaysia, stingless bees can be categorised into two genera: Melipona and Trigona, known as “kelulut”. The high demand for kelulut honey boosts the production of the honey industry. Previous studies reported that stingless bee (kelulut) honey and its products were contaminated with pathogenic bacteria during harvesting and processing. This research aims to isolate and identify the pathogenic bacteria in kelulut honey. Forty-eight samples of kelulut honey (open and closed pot) and propolis were obtained from selected farms in Terengganu by focusing on a major stingless bee species available in Malaysia, Heterotrigona itama. In addition, the swabbing technique was done on the wooden beehive of the kelulut to evaluate the environmental contamination. The pathogenic bacteria were isolated using specifically selected agar, such as Bacillus cereus agar (for B. cereus), Baird-Parker agar (for Staphylococcus aureus), and MacConkey agar (for other pathogenic bacteria), which were confirmed through a biochemical test. All samples were analysed, and the results showed that B. cereus (7/48), Pseudomonas aeruginosa (10/48), Pantoea spp. (11/48), Serratia plymuthica (6/48), and S. aureus (9/48) were obtained in the samples. This study indicates that kelulut honey was contaminated with B. cereus, P. aeruginosa, Pantoea spp., S. plymuthica, and S. aureus. Isolated pathogenic bacteria may exist in the kelulut honey through food handlers, utensils, and the environment. Hence, the stakeholders should strictly follow good standard operating procedures and guidelines by the kelulut honey industry to prevent foodborne illness.","PeriodicalId":19890,"journal":{"name":"Pertanika Journal of Tropical Agricultural Science","volume":" ","pages":""},"PeriodicalIF":0.6,"publicationDate":"2023-07-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"47603865","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
A. G. Gashua, Z. Sulaiman, Martini Mohammad Yusoff, Mohd Yusoff Abd. Samad, M. Ramlan, Shafar Jefri Mokhatar
Rubber is currently the second major industrial crop in Malaysia after oil palm. The use of bokashi fertilizer (BF) on industrial crops is still not popular, and farmers rely mostly on chemical fertilizers (CFs) that are costly and hazardous to the environment. This research was conducted at Hevea plantation, Universiti Putra Malaysia, between August 2020–October 2021. The study was to assess the short-term effects of BF with reduced NPK fertilization on soil fertility, growth, and yield of rubber. Seven treatments (T) were involved. T1, T2, and T3 denote 4, 8, and 12 kg BF per pit. T4 was 1 kg NPK as control, whereas T5, T6, and T7 denote 4, 8, and 12 kg BF + 500 g NPK per pit, respectively. The variables observed included total nitrogen (TN%), total phosphorus (TP%), organic carbon (OC%), organic matter (OM%), exchangeable cations, microbial counts, tree girth, and dry rubber yield. The major findings indicated that applying 12 kg BF has raised the soil’s TN%, OC%, and OM% by 165, 171.4, and 172.0%, respectively, compared to NPK control. Also, adding 4 kg BF + 500 g NPK has increased the soil’s cation exchange capacity and TP% values by 107.8 and 42.9%, respectively, compared to the control. Adding sole bokashi increased the bacterial population by 22.2–133.3%. Rubber yield was better on trees treated with 12 kg BF, though this did not differ significantly from other treatments. Therefore, applying 12 kg BF or 4 kg BF + 500 g NPK can improve soil fertility and save costs from using inorganic fertilizer by at least 50%.
橡胶目前是马来西亚仅次于油棕的第二大工业作物。在工业作物上使用博卡施化肥（BF）仍然不受欢迎，农民主要依赖昂贵且对环境有害的化肥。这项研究于2020年8月至2021年10月在马来西亚普特拉大学橡胶树种植园进行。本研究旨在评估BF减少NPK施肥对土壤肥力、生长和橡胶产量的短期影响。涉及7种治疗（T）。T1、T2和T3表示每个坑4、8和12 kg BF。T4为1kg NPK作为对照，而T5、T6和T7分别表示每个凹坑4、8和12kg BF+500g NPK。观察到的变量包括总氮（TN%）、总磷（TP%）、有机碳（OC%）、有机组分（OM%）、可交换阳离子、微生物计数、树木周长和干橡胶产量。主要研究结果表明，与NPK对照相比，施用12kg BF可使土壤的TN%、OC%和OM%分别提高165、171.4和172.0%。此外，与对照相比，添加4 kg BF+500 g NPK可使土壤的阳离子交换能力和TP%值分别增加107.8%和42.9%。添加sole bokashi可使细菌数量增加22.2–133.3%。用12kg BF处理的树木橡胶产量更好，尽管这与其他处理没有显著差异。因此，施用12 kg BF或4 kg BF+500 g NPK可以提高土壤肥力，并至少节省50%的无机肥使用成本。
{"title":"Short-Term Effects of Bokashi Fertilizer with Reduced NPK Fertilization on Soil Fertility, Growth, and Yield of Rubber Trees","authors":"A. G. Gashua, Z. Sulaiman, Martini Mohammad Yusoff, Mohd Yusoff Abd. Samad, M. Ramlan, Shafar Jefri Mokhatar","doi":"10.47836/pjtas.46.3.07","DOIUrl":"https://doi.org/10.47836/pjtas.46.3.07","url":null,"abstract":"Rubber is currently the second major industrial crop in Malaysia after oil palm. The use of bokashi fertilizer (BF) on industrial crops is still not popular, and farmers rely mostly on chemical fertilizers (CFs) that are costly and hazardous to the environment. This research was conducted at Hevea plantation, Universiti Putra Malaysia, between August 2020–October 2021. The study was to assess the short-term effects of BF with reduced NPK fertilization on soil fertility, growth, and yield of rubber. Seven treatments (T) were involved. T1, T2, and T3 denote 4, 8, and 12 kg BF per pit. T4 was 1 kg NPK as control, whereas T5, T6, and T7 denote 4, 8, and 12 kg BF + 500 g NPK per pit, respectively. The variables observed included total nitrogen (TN%), total phosphorus (TP%), organic carbon (OC%), organic matter (OM%), exchangeable cations, microbial counts, tree girth, and dry rubber yield. The major findings indicated that applying 12 kg BF has raised the soil’s TN%, OC%, and OM% by 165, 171.4, and 172.0%, respectively, compared to NPK control. Also, adding 4 kg BF + 500 g NPK has increased the soil’s cation exchange capacity and TP% values by 107.8 and 42.9%, respectively, compared to the control. Adding sole bokashi increased the bacterial population by 22.2–133.3%. Rubber yield was better on trees treated with 12 kg BF, though this did not differ significantly from other treatments. Therefore, applying 12 kg BF or 4 kg BF + 500 g NPK can improve soil fertility and save costs from using inorganic fertilizer by at least 50%.","PeriodicalId":19890,"journal":{"name":"Pertanika Journal of Tropical Agricultural Science","volume":" ","pages":""},"PeriodicalIF":0.6,"publicationDate":"2023-06-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"49164310","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Siti Nor Azizah Mahamud, T. M. Hamisu, Juan Luis Criado Rius, Shyong Wey Ong, A. Omar
This research aims to evaluate the immunogenicity of different doses of HIPRAVIAR® BPL2 inactivated Newcastle disease virus (NDV) LaSota vaccine. Specific-pathogen-free day-old chicks were divided into 3 different groups, and each group was vaccinated subcutaneously with the vaccine dose of 0.1, 0.2, and 0.5 ml, respectively. Blood samples were collected to measure NDV-specific antibody titers using a hemagglutination inhibition (HI) test and enzyme-linked immunosorbent assay (ELISA). The HI result showed that birds vaccinated with 0.5 ml HIPRAVIAR® BPL2 vaccine showed an increased statistically significant antibody titer compared to the other doses. Similarly, the ELISA result corroborated the HI finding. No significant difference between the results was detected when the antibody titers were measured using two ELISA kits, Biocheck CK116, and CIVTEST® AVI NDV. The percentage antibody-positive test based on HI amongst the different days post-vaccination showed that all the birds were positive from 28 to 42 days following vaccination with HIPRAVIAR® BPL2 0.5 ml (group D), whereas the highest percentage of antibody positivity were 80% and 70% at 42 days post-vaccination with HIPRAVIAR® BPL2 0.1 ml (group B) and HIPRAVIAR® BPL2 0.2 ml (group C), respectively. In conclusion, besides the difference in seroconversion, all the vaccine doses used had important levels of seroconversion and positivity.
本研究旨在评估不同剂量的HIPRAVIAR®BPL2灭活新城疫病毒（NDV）LaSota疫苗的免疫原性。将无特定病原体的日龄雏鸡分为3组，每组分别皮下接种0.1、0.2和0.5ml的疫苗。采集血样，使用血凝抑制（HI）试验和酶联免疫吸附试验（ELISA）测量NDV特异性抗体滴度。HI结果显示，与其他剂量相比，接种0.5 ml HIPRAVIAR®BPL2疫苗的鸟类显示出具有统计学意义的抗体滴度增加。同样，ELISA结果证实了HI的发现。当使用两种ELISA试剂盒Biocheck CK116和CIVTEST®AVI NDV测量抗体滴度时，结果之间没有显著差异。基于HI在接种后不同天数的抗体阳性百分比测试显示，所有鸟类在接种0.5 ml HIPRAVIAR®BPL2疫苗后28至42天（D组）均呈阳性，而在接种0.1 ml HIPRAVIIAR®BPL20.1 ml（B组）和0.2 ml HIPRAVISAR®BPL10.2 ml（C组）后42天抗体阳性百分比最高，分别为80%和70%，分别地总之，除了血清转化率的差异外，所有使用的疫苗剂量都具有重要的血清转化率和阳性率。
{"title":"Immunity Evaluation of Inactivated Newcastle Disease Virus Vaccine Inoculated at Different Doses in Day-old Specific-Pathogen-Free Chicks","authors":"Siti Nor Azizah Mahamud, T. M. Hamisu, Juan Luis Criado Rius, Shyong Wey Ong, A. Omar","doi":"10.47836/pjtas.46.3.03","DOIUrl":"https://doi.org/10.47836/pjtas.46.3.03","url":null,"abstract":"This research aims to evaluate the immunogenicity of different doses of HIPRAVIAR® BPL2 inactivated Newcastle disease virus (NDV) LaSota vaccine. Specific-pathogen-free day-old chicks were divided into 3 different groups, and each group was vaccinated subcutaneously with the vaccine dose of 0.1, 0.2, and 0.5 ml, respectively. Blood samples were collected to measure NDV-specific antibody titers using a hemagglutination inhibition (HI) test and enzyme-linked immunosorbent assay (ELISA). The HI result showed that birds vaccinated with 0.5 ml HIPRAVIAR® BPL2 vaccine showed an increased statistically significant antibody titer compared to the other doses. Similarly, the ELISA result corroborated the HI finding. No significant difference between the results was detected when the antibody titers were measured using two ELISA kits, Biocheck CK116, and CIVTEST® AVI NDV. The percentage antibody-positive test based on HI amongst the different days post-vaccination showed that all the birds were positive from 28 to 42 days following vaccination with HIPRAVIAR® BPL2 0.5 ml (group D), whereas the highest percentage of antibody positivity were 80% and 70% at 42 days post-vaccination with HIPRAVIAR® BPL2 0.1 ml (group B) and HIPRAVIAR® BPL2 0.2 ml (group C), respectively. In conclusion, besides the difference in seroconversion, all the vaccine doses used had important levels of seroconversion and positivity.","PeriodicalId":19890,"journal":{"name":"Pertanika Journal of Tropical Agricultural Science","volume":" ","pages":""},"PeriodicalIF":0.6,"publicationDate":"2023-06-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"46197436","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
T. Joko, Arzaq Prabantoro Yuantomoputro, Restu Indrawati, A. Soffan, S. Subandiyah
The significant decrease in clove production in Indonesia is mainly due to the Sumatra disease caused by bacterial Ralstonia syzygii subsp. syzygii. It is necessary to manage the disease broadly based on disease detection in the field and the laboratory. This study aims to determine the technique for detecting the distribution pattern of Sumatra disease using geographic information systems and validate the presence of R. syzygii subsp. syzygii in clove plant tissues by molecular analyses based on the endoglucanase gene. This research was conducted by acquiring aerial photos using uncrewed aerial vehicles processed using photogrammetric techniques to produce geographic information system outputs as a thematic map of the clove Sumatra disease distribution pattern. The plant samples were collected for molecular analysis of the pathogens causing clove Sumatra disease in the laboratory. DNA extraction was performed and amplified by polymerase chain reaction (PCR) using UGMRss-specific primers followed by Sanger sequencing. The aerial photo images showed that the distribution of clove Sumatra disease has a random pattern, which tends to spread between healthy and diseased plants. Furthermore, the PCR and phylogenetic analyses revealed that all symptomatic clove plant samples (leaves, twigs, and roots) were positively infected by R. syzygii subsp. syzygii.
{"title":"Field and Laboratory Detection of Clove Sumatra Disease Caused by Ralstonia syzygii subsp. syzygii in Java, Indonesia","authors":"T. Joko, Arzaq Prabantoro Yuantomoputro, Restu Indrawati, A. Soffan, S. Subandiyah","doi":"10.47836/pjtas.46.3.05","DOIUrl":"https://doi.org/10.47836/pjtas.46.3.05","url":null,"abstract":"The significant decrease in clove production in Indonesia is mainly due to the Sumatra disease caused by bacterial Ralstonia syzygii subsp. syzygii. It is necessary to manage the disease broadly based on disease detection in the field and the laboratory. This study aims to determine the technique for detecting the distribution pattern of Sumatra disease using geographic information systems and validate the presence of R. syzygii subsp. syzygii in clove plant tissues by molecular analyses based on the endoglucanase gene. This research was conducted by acquiring aerial photos using uncrewed aerial vehicles processed using photogrammetric techniques to produce geographic information system outputs as a thematic map of the clove Sumatra disease distribution pattern. The plant samples were collected for molecular analysis of the pathogens causing clove Sumatra disease in the laboratory. DNA extraction was performed and amplified by polymerase chain reaction (PCR) using UGMRss-specific primers followed by Sanger sequencing. The aerial photo images showed that the distribution of clove Sumatra disease has a random pattern, which tends to spread between healthy and diseased plants. Furthermore, the PCR and phylogenetic analyses revealed that all symptomatic clove plant samples (leaves, twigs, and roots) were positively infected by R. syzygii subsp. syzygii.","PeriodicalId":19890,"journal":{"name":"Pertanika Journal of Tropical Agricultural Science","volume":" ","pages":""},"PeriodicalIF":0.6,"publicationDate":"2023-06-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"46493289","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Fathiya Khairiya, F. Dwivany, S. Suhandono, Sofia Safitri Hessel, I. Zainuddin, T. Tallei
Lectins are a family of proteins that can recognize and bind specific carbohydrates. Plant lectins play various roles in plant defense and can be utilized as insecticidal, antibacterial, antifungal, and antiviral agents. This study compares genes, proteins, and carbohydrate-binding motifs between 15 plant lectins using in silico methods. The lectin genes of Artocarpus hypargyreus Hance, Hordeum vulgare var. Betzes, Triticum aestivum L. cv. Marshall, Galanthus nivalis L., Allium sativum L., Phaseolus vulgaris, Lens culinaris subsp. tomentosus, Robinia pseudoacacia, Glycine max, Cicer arietinum, Pisum sativum, Canavalia ensiformis, Amaranthus caudatus, Amaranthus hypochondriacus, and Musa acuminata subsp. malaccensis were obtained from National Center for Biotechnology Information and Banana Genome Hub. The gene comparison results revealed different characteristics of the 15 plant lectin genes, with A. hypargyreus having the shortest lectin gene and G. max having the longest. Overall, the 15 plant lectin genes have 1–3 exons. Domain predictions revealed the presence of five domains: jacalin, chitin_bind_1, B_lectin, legume lectin, and agglutinin. Furthermore, there were 2 protein sequences from the jacalin domain, 2 protein sequences from the chitin_bind_I domain, 2 protein sequences from the B_lectin domain, and 4 protein sequences from the legume lectin domains that have complete carbohydrate-binding motifs compared to consensus motifs from literature. The data obtained from this study has not been previously reported and can be utilized for future lectin protein production with synthetic biology approaches. This method will allow scientists to obtain plant bioparts for lectin production using a heterologous system, even without plant samples.
{"title":"In silico Comparative Analysis of Gene and Protein of Plant Lectins","authors":"Fathiya Khairiya, F. Dwivany, S. Suhandono, Sofia Safitri Hessel, I. Zainuddin, T. Tallei","doi":"10.47836/pjtas.46.3.06","DOIUrl":"https://doi.org/10.47836/pjtas.46.3.06","url":null,"abstract":"Lectins are a family of proteins that can recognize and bind specific carbohydrates. Plant lectins play various roles in plant defense and can be utilized as insecticidal, antibacterial, antifungal, and antiviral agents. This study compares genes, proteins, and carbohydrate-binding motifs between 15 plant lectins using in silico methods. The lectin genes of Artocarpus hypargyreus Hance, Hordeum vulgare var. Betzes, Triticum aestivum L. cv. Marshall, Galanthus nivalis L., Allium sativum L., Phaseolus vulgaris, Lens culinaris subsp. tomentosus, Robinia pseudoacacia, Glycine max, Cicer arietinum, Pisum sativum, Canavalia ensiformis, Amaranthus caudatus, Amaranthus hypochondriacus, and Musa acuminata subsp. malaccensis were obtained from National Center for Biotechnology Information and Banana Genome Hub. The gene comparison results revealed different characteristics of the 15 plant lectin genes, with A. hypargyreus having the shortest lectin gene and G. max having the longest. Overall, the 15 plant lectin genes have 1–3 exons. Domain predictions revealed the presence of five domains: jacalin, chitin_bind_1, B_lectin, legume lectin, and agglutinin. Furthermore, there were 2 protein sequences from the jacalin domain, 2 protein sequences from the chitin_bind_I domain, 2 protein sequences from the B_lectin domain, and 4 protein sequences from the legume lectin domains that have complete carbohydrate-binding motifs compared to consensus motifs from literature. The data obtained from this study has not been previously reported and can be utilized for future lectin protein production with synthetic biology approaches. This method will allow scientists to obtain plant bioparts for lectin production using a heterologous system, even without plant samples.","PeriodicalId":19890,"journal":{"name":"Pertanika Journal of Tropical Agricultural Science","volume":" ","pages":""},"PeriodicalIF":0.6,"publicationDate":"2023-06-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"42048332","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Sibel Tan, S. Kasim, Martini Mohammad Yusoff, Syaharudin Zaibon, S. Raguraj
The production cost of chili in the fertigation system has increased recently due to the high cost of water-soluble fertilizers used in the system. Laundry greywater and biodegradable vegetable waste are rich in nutrients essential for plant growth. Thus, this research aims to investigate the effects of greywater organomineral fertilizer (OMF) on the chili plants’ growth and yield performance in the fertigation system. The experiment was laid out in a completely randomized design under the rain shelter. OMF produced from laundry water and vegetable waste was applied with chemical fertilizer (CF) in different ratios, including 100% CF (T1, control), 75% CF + 25% OMF (T2), 50% CF + 50% OMF (T3), 25% CF + 75% OMF (T4), and 100% OMF (T5). Results showed that the combined use of CF and OMF produced non-significantly different chili plants from those solely treated by CF. Interestingly, chili plants treated with 50% CF and 50% OMF increased the yield by 4.71% compared to CF. Chili plants treated with 25% and 50% OMF showed non-significantly different plant height, stem diameter, plant dry weight, fruit number, and proximate composition of fruits over those treated with 100% CF. Solely application of OMF produced similar chili as CF in terms of fruit quality. The present study shows that plant performance and yield of chili were improved after the application of CF and OMF at a ratio of 50:50. It can be concluded that OMF has the potential to be used as an alternative for replacing 50% of chemical fertilizer in chili fertigation system without affecting its growth and yield.
{"title":"Effects of Greywater Organomineral Liquid Fertilizer on the Growth, Yield Performance, and Proximate Composition of Chili (Capsicum annum L.)","authors":"Sibel Tan, S. Kasim, Martini Mohammad Yusoff, Syaharudin Zaibon, S. Raguraj","doi":"10.47836/pjtas.46.3.02","DOIUrl":"https://doi.org/10.47836/pjtas.46.3.02","url":null,"abstract":"The production cost of chili in the fertigation system has increased recently due to the high cost of water-soluble fertilizers used in the system. Laundry greywater and biodegradable vegetable waste are rich in nutrients essential for plant growth. Thus, this research aims to investigate the effects of greywater organomineral fertilizer (OMF) on the chili plants’ growth and yield performance in the fertigation system. The experiment was laid out in a completely randomized design under the rain shelter. OMF produced from laundry water and vegetable waste was applied with chemical fertilizer (CF) in different ratios, including 100% CF (T1, control), 75% CF + 25% OMF (T2), 50% CF + 50% OMF (T3), 25% CF + 75% OMF (T4), and 100% OMF (T5). Results showed that the combined use of CF and OMF produced non-significantly different chili plants from those solely treated by CF. Interestingly, chili plants treated with 50% CF and 50% OMF increased the yield by 4.71% compared to CF. Chili plants treated with 25% and 50% OMF showed non-significantly different plant height, stem diameter, plant dry weight, fruit number, and proximate composition of fruits over those treated with 100% CF. Solely application of OMF produced similar chili as CF in terms of fruit quality. The present study shows that plant performance and yield of chili were improved after the application of CF and OMF at a ratio of 50:50. It can be concluded that OMF has the potential to be used as an alternative for replacing 50% of chemical fertilizer in chili fertigation system without affecting its growth and yield.","PeriodicalId":19890,"journal":{"name":"Pertanika Journal of Tropical Agricultural Science","volume":" ","pages":""},"PeriodicalIF":0.6,"publicationDate":"2023-06-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"43606465","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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