Pub Date : 2025-02-01DOI: 10.1016/j.pdpdt.2024.104433
Ali Neshastehriz , Zeinab Hormozi-Moghaddam , Zahra Abedi Kichi , Seyedeh Mona Taheri , Seyed Mohammad Amini , Amir Aghaei
Background
Acoustic cavitation is a foundational mechanism in ultrasound therapy, primarily through inertial cavitation resulting from microbubble collapse. Sonodynamic therapy, with inertial acoustic cavitation threshold and low-dose radiation in the presence of sensitizers, may provide significant effects for cancer treatment, potentially overcoming resistance encountered with single therapies.
Methods
MCF7 breast cancer cells were subjected to sonodynamic therapy either alone or combined with ionizing radiation, gold nanoparticles coated with apigenin, and methylene blue. Several parameters were evaluated, including reactive oxygen species (ROS) generation and colonization. Additionally, the investigation included assessing the long non-coding RNA (lncRNA) PTV1 with miRNA1204 and related genes using Real-Time PCR.
Results
Sonodynamic therapy at a mechanical index of 0.31 as acoustic cavitation threshold increased intracellular ROS. Combining sonodynamic therapy and 2 Gy X-ray radiation with methylene blue and gold nanoparticles coated with apigenin significantly decreased plating efficiency (4.44±1.69), and survival fraction (2.75±1.98) compared with control (Ctrl.) (98.77±4.49) and (97.59± 2.94), respectively. This was associated with a marked increase in ROS with a mean fluorescence intensity of 20,576.2 ± 4.6 (>4.5 times). The combined treatment also increased p53 expression and decreased the expression of PVT1, miR-1204, and related genes.
Conclusion
Sonodynamic therapy in inertial acoustic cavitation threshold, combined with ionizing radiation in the presence of biocompatible nanoparticles, could enhance the therapeutic effects on the miR-1204, derived from lncRNA PVT1, that functions as an oncogenic microRNA in breast cancer. This approach has the potential to overcome treatment resistance encountered with single therapies.
{"title":"Overcoming breast cancer cell treatment resistance by optimizing sonodynamic therapy and radiation sensitizers on lncRNA PVT1 and miR-1204 expression","authors":"Ali Neshastehriz , Zeinab Hormozi-Moghaddam , Zahra Abedi Kichi , Seyedeh Mona Taheri , Seyed Mohammad Amini , Amir Aghaei","doi":"10.1016/j.pdpdt.2024.104433","DOIUrl":"10.1016/j.pdpdt.2024.104433","url":null,"abstract":"<div><h3>Background</h3><div>Acoustic cavitation is a foundational mechanism in ultrasound therapy, primarily through inertial cavitation resulting from microbubble collapse. Sonodynamic therapy, with inertial acoustic cavitation threshold and low-dose radiation in the presence of sensitizers, may provide significant effects for cancer treatment, potentially overcoming resistance encountered with single therapies.</div></div><div><h3>Methods</h3><div>MCF7 breast cancer cells were subjected to sonodynamic therapy either alone or combined with ionizing radiation, gold nanoparticles coated with apigenin, and methylene blue. Several parameters were evaluated, including reactive oxygen species (ROS) generation and colonization. Additionally, the investigation included assessing the long non-coding RNA (lncRNA) PTV1 with miRNA1204 and related genes using Real-Time PCR.</div></div><div><h3>Results</h3><div>Sonodynamic therapy at a mechanical index of 0.31 as acoustic cavitation threshold increased intracellular ROS. Combining sonodynamic therapy and 2 Gy X-ray radiation with methylene blue and gold nanoparticles coated with apigenin significantly decreased plating efficiency (4.44±1.69), and survival fraction (2.75±1.98) compared with control (Ctrl.) (98.77±4.49) and (97.59± 2.94), respectively. This was associated with a marked increase in ROS with a mean fluorescence intensity of 20,576.2 ± 4.6 (>4.5 times). The combined treatment also increased p53 expression and decreased the expression of PVT1, miR-1204, and related genes.</div></div><div><h3>Conclusion</h3><div>Sonodynamic therapy in inertial acoustic cavitation threshold, combined with ionizing radiation in the presence of biocompatible nanoparticles, could enhance the therapeutic effects on the miR-1204, derived from lncRNA PVT1, that functions as an oncogenic microRNA in breast cancer. This approach has the potential to overcome treatment resistance encountered with single therapies.</div></div>","PeriodicalId":20141,"journal":{"name":"Photodiagnosis and Photodynamic Therapy","volume":"51 ","pages":"Article 104433"},"PeriodicalIF":3.1,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142793072","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Since recurrences and new AKs commonly are observed after treatment long-term effects of field-directed therapies for AKs are crucial. Thus, we evaluated the 6- and 12-month efficacy and satisfaction of sequential topical 5-fluorouracil (5-FU) and daylight PDT (dPDT) with dPDT alone.
Methods
In a randomized intra-individual study patients with multiple AKs in the face and scalp were treated with 4 % 5-fluorouracil cream twice daily for 7 days on one side of the scalp/face, followed by dPDT on both sides. Lesion clearance rate, lesion recurrence rate and number of new lesions were evaluated at 6- and 12-month after treatment.
Results
58/60 completed 6-month and 55/60 completed 12-month follow-up. Pre-treatment with topical 5-FU improved the long-term efficacy of dPDT, especially for moderately thick AKs, with a lesion clearance of 72 % at 12-month compared to 48 % after dPDT alone (p = 0.017). The recurrence rate at 12-month was 15 % for combination treatment and 20 % for dPDT alone (0.042). Most patients were highly satisfied with both treatments.
Conclusions
Sequential treatment with short-term topical 5-FU followed by dPDT maintained a higher complete lesion clearance than dPDT alone at both 6- and 12-month follow-ups, with fewer new AKs developing after combination therapy.
{"title":"Is the benefit of sequential 5-fluorouracil and daylight photodynamic therapy versus daylight photodynamic therapy alone sustained over time? – 12-month follow-up of a randomized controlled trial","authors":"Stine Regin Wiegell , Gabriella Fredman , Flemming Andersen , Peter Bjerring , Uwe Paasch , Merete Haedersdal","doi":"10.1016/j.pdpdt.2024.104445","DOIUrl":"10.1016/j.pdpdt.2024.104445","url":null,"abstract":"<div><h3>Background</h3><div>Since recurrences and new AKs commonly are observed after treatment long-term effects of field-directed therapies for AKs are crucial. Thus, we evaluated the 6- and 12-month efficacy and satisfaction of sequential topical 5-fluorouracil (5-FU) and daylight PDT (dPDT) with dPDT alone.</div></div><div><h3>Methods</h3><div>In a randomized intra-individual study patients with multiple AKs in the face and scalp were treated with 4 % 5-fluorouracil cream twice daily for 7 days on one side of the scalp/face, followed by dPDT on both sides. Lesion clearance rate, lesion recurrence rate and number of new lesions were evaluated at 6- and 12-month after treatment.</div></div><div><h3>Results</h3><div>58/60 completed 6-month and 55/60 completed 12-month follow-up. Pre-treatment with topical 5-FU improved the long-term efficacy of dPDT, especially for moderately thick AKs, with a lesion clearance of 72 % at 12-month compared to 48 % after dPDT alone (<em>p</em> = 0.017). The recurrence rate at 12-month was 15 % for combination treatment and 20 % for dPDT alone (0.042). Most patients were highly satisfied with both treatments.</div></div><div><h3>Conclusions</h3><div>Sequential treatment with short-term topical 5-FU followed by dPDT maintained a higher complete lesion clearance than dPDT alone at both 6- and 12-month follow-ups, with fewer new AKs developing after combination therapy.</div></div>","PeriodicalId":20141,"journal":{"name":"Photodiagnosis and Photodynamic Therapy","volume":"51 ","pages":"Article 104445"},"PeriodicalIF":3.1,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142824941","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2025-02-01DOI: 10.1016/j.pdpdt.2024.104443
Çisil Erkan Pota , Özge Ekin Geçer Şerifoğlu , Aslı Çetinkaya Yaprak , Hatice Deniz İlhan , Adil Boz
Purpose
To assess the anatomical and functional outcomes in patients who underwent surgery for canalicular laceration and did not experience significant epiphora, and to compare these outcomes with contralateral uninjured eye.
Method
Dacryoscintigraphy, anterior segment optical coherence tomography (OCT), MUNK scores, and a satisfaction questionnaire were administered to 24 patients who had canalicular laceration repair without significant epiphora and had a minimum of 6 months of follow-up. Tear meniscus height (TMH), depth (TMD), and area (TMA) were measured using anterior segment OCT and compared with the values in the uninjured eye.
Results
Sixteen patients (67 %) had lower, 7 (29 %) had upper canaliculus, and one (4 %) had lacerations in both canaliculi. Five patients (20.83 %) had concomitant orbital fractures, and three patients (12.5 %) underwent additional repair for corneoscleral laceration. Bicanalicular silicone tube intubation was performed in one patient, while Mini-Monoka stent intubation was used for all other patients. The mean follow-up period was 41 ± 22.3 months, and the mean interval between trauma and surgery was 30.2 ± 29 h. The mean duration until tube removal was 3 ± 2.54 months. The MUNK score was 1 in eight patients (33 %) and 0 in sixteen patients (67 %). Canalicular lacerations occurred due to home accidents in 8 patients, work accidents in 4, assaults in 5, and traffic accidents in 7.
Dacryoscintigraphy showed drainage of tracer into the nasal cavity in dynamic imaging for 12 patients. Five patients had retention in the sac, four had prolonged and reduced drainage, one had reduced drainage, and two showed no drainage in the first and second hour images. In the operated eye, the mean tear meniscus height (TMH) was 279.6 µm, depth (TMD) was 215 µm, and area (TMA) was 28.9 µm². In the healthy eye, the mean TMH was 221.5 µm, TMD was 152.5 µm, and TMA was 15.3 µm². The anterior segment OCT values for the affected eye were statistically significantly higher than those for the healthy eye (p = 0.044 for TMH, p = 0.003 for TMD, p = 0.006 for TMA). TMD and TMA were statistically significantly higher in patients with a MUNK score of 1 (p = 0.019 for TMD, p = 0.05 for TMA). Abnormal dacryoscintigraphy results were more common in patients with globe injuries requiring additional surgery.
Conclusion
Although patients did not report epiphora after canalicular laceration repair, our observations indicated potential functional and anatomical differences in dacryoscintigraphy and anterior segment OCT compared to their healthy eyes. We believe that close follow-up is crucial to identify and address any issues that may arise in the future following surgery.
{"title":"Evaluation of anatomical and functional success in canalicular laceration repair using anterior segment optical coherence tomography and dacryoscintigraphy","authors":"Çisil Erkan Pota , Özge Ekin Geçer Şerifoğlu , Aslı Çetinkaya Yaprak , Hatice Deniz İlhan , Adil Boz","doi":"10.1016/j.pdpdt.2024.104443","DOIUrl":"10.1016/j.pdpdt.2024.104443","url":null,"abstract":"<div><h3>Purpose</h3><div>To assess the anatomical and functional outcomes in patients who underwent surgery for canalicular laceration and did not experience significant epiphora, and to compare these outcomes with contralateral uninjured eye.</div></div><div><h3>Method</h3><div>Dacryoscintigraphy, anterior segment optical coherence tomography (OCT), MUNK scores, and a satisfaction questionnaire were administered to 24 patients who had canalicular laceration repair without significant epiphora and had a minimum of 6 months of follow-up. Tear meniscus height (TMH), depth (TMD), and area (TMA) were measured using anterior segment OCT and compared with the values in the uninjured eye.</div></div><div><h3>Results</h3><div>Sixteen patients (67 %) had lower, 7 (29 %) had upper canaliculus, and one (4 %) had lacerations in both canaliculi. Five patients (20.83 %) had concomitant orbital fractures, and three patients (12.5 %) underwent additional repair for corneoscleral laceration. Bicanalicular silicone tube intubation was performed in one patient, while Mini-Monoka stent intubation was used for all other patients. The mean follow-up period was 41 ± 22.3 months, and the mean interval between trauma and surgery was 30.2 ± 29 h. The mean duration until tube removal was 3 ± 2.54 months. The MUNK score was 1 in eight patients (33 %) and 0 in sixteen patients (67 %). Canalicular lacerations occurred due to home accidents in 8 patients, work accidents in 4, assaults in 5, and traffic accidents in 7.</div><div>Dacryoscintigraphy showed drainage of tracer into the nasal cavity in dynamic imaging for 12 patients. Five patients had retention in the sac, four had prolonged and reduced drainage, one had reduced drainage, and two showed no drainage in the first and second hour images. In the operated eye, the mean tear meniscus height (TMH) was 279.6 µm, depth (TMD) was 215 µm, and area (TMA) was 28.9 µm². In the healthy eye, the mean TMH was 221.5 µm, TMD was 152.5 µm, and TMA was 15.3 µm². The anterior segment OCT values for the affected eye were statistically significantly higher than those for the healthy eye (<em>p</em> = 0.044 for TMH, <em>p</em> = 0.003 for TMD, <em>p</em> = 0.006 for TMA). TMD and TMA were statistically significantly higher in patients with a MUNK score of 1 (<em>p</em> = 0.019 for TMD, <em>p</em> = 0.05 for TMA). Abnormal dacryoscintigraphy results were more common in patients with globe injuries requiring additional surgery.</div></div><div><h3>Conclusion</h3><div>Although patients did not report epiphora after canalicular laceration repair, our observations indicated potential functional and anatomical differences in dacryoscintigraphy and anterior segment OCT compared to their healthy eyes. We believe that close follow-up is crucial to identify and address any issues that may arise in the future following surgery.</div></div>","PeriodicalId":20141,"journal":{"name":"Photodiagnosis and Photodynamic Therapy","volume":"51 ","pages":"Article 104443"},"PeriodicalIF":3.1,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142831539","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2025-02-01DOI: 10.1016/j.pdpdt.2024.104456
Mona Mahmoud , Peter Richter , Philipp Arnold , Michael Lebert , Andreas Burkovski
Pseudomonas aeruginosa is a hard-to-treat human pathogen for which new antimicrobial agents are urgently needed. P. aeruginosa is known for forming biofilms, a complex aggregate of bacteria embedded in a self-generated protective matrix that enhance its resistance to antibiotics and the immune system. Within the biofilm, persister cells, sub-populations of slow-growing or growth-arrested cells, are associated with recalcitrance of infections and antibiotic treatment failure. Here, we investigate the influence of the anionic photosensitiser chlorophyllin (CHL)1 exposed to red light alone and in combination with an activator of the mechanosensitive channels butylparaben (BP) on P. aeruginosa growing cells, persister cells, and biofilms. Antimicrobial susceptibility tests were performed using the broth microdilution checkerboard method. Serine hydroxamate (SHX) was used for the induction of persister cells. Under illumination, a combination of CHL (250 µg/ml) and BP (97.12 µg/ml) reduced the number of growing cells and persister cells by log10 and log10, respectively after 30 min of exposure at 79 J/cm2. A higher concentration of BP (194.23 µg/ml) or longer exposure time (60 min at 158 J/cm2) effectively eliminated approximately ≥99.99 % of growing and persister cells. Visual evidence from confocal and TEM images illustrates the influence of CHL and red light, which intensifies when combined with BP. Nevertheless, the addition of BP did not enhance the efficacy of CHL against biofilms; CHL (500 µg/ml) reduced biofilm viability by 2.6 log10 at 791 J/cm2. No toxicity has been observed in darkness. This study highlights the potential antimicrobial effect of CHL against P. aeruginosa.
{"title":"Antimicrobial photodynamic inactivation of Pseudomonas aeruginosa persister cells and biofilms","authors":"Mona Mahmoud , Peter Richter , Philipp Arnold , Michael Lebert , Andreas Burkovski","doi":"10.1016/j.pdpdt.2024.104456","DOIUrl":"10.1016/j.pdpdt.2024.104456","url":null,"abstract":"<div><div><em>Pseudomonas aeruginosa</em> is a hard-to-treat human pathogen for which new antimicrobial agents are urgently needed. <em>P. aeruginosa</em> is known for forming biofilms, a complex aggregate of bacteria embedded in a self-generated protective matrix that enhance its resistance to antibiotics and the immune system. Within the biofilm, persister cells, sub-populations of slow-growing or growth-arrested cells, are associated with recalcitrance of infections and antibiotic treatment failure. Here, we investigate the influence of the anionic photosensitiser chlorophyllin (CHL)<span><span><sup>1</sup></span></span> exposed to red light alone and in combination with an activator of the mechanosensitive channels butylparaben (BP) on <em>P. aeruginosa</em> growing cells, persister cells, and biofilms. Antimicrobial susceptibility tests were performed using the broth microdilution checkerboard method. Serine hydroxamate (SHX) was used for the induction of persister cells. Under illumination, a combination of CHL (250 µg/ml) and BP (97.12 µg/ml) reduced the number of growing cells and persister cells by <span><math><mrow><mn>2.2</mn><mo>±</mo><mn>0.46</mn></mrow></math></span> log<sub>10</sub> and <span><math><mrow><mn>1.7</mn><mo>±</mo><mn>0.15</mn></mrow></math></span> log<sub>10</sub>, respectively after 30 min of exposure at 79 J/cm<sup>2</sup>. A higher concentration of BP (194.23 µg/ml) or longer exposure time (60 min at 158 J/cm<sup>2</sup>) effectively eliminated approximately ≥99.99 % of growing and persister cells. Visual evidence from confocal and TEM images illustrates the influence of CHL and red light, which intensifies when combined with BP. Nevertheless, the addition of BP did not enhance the efficacy of CHL against biofilms; CHL (500 µg/ml) reduced biofilm viability by 2.6 log<sub>10</sub> at 791 J/cm<sup>2</sup>. No toxicity has been observed in darkness. This study highlights the potential antimicrobial effect of CHL against <em>P. aeruginosa</em>.</div></div>","PeriodicalId":20141,"journal":{"name":"Photodiagnosis and Photodynamic Therapy","volume":"51 ","pages":"Article 104456"},"PeriodicalIF":3.1,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142904661","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Rosacea is a chronic, inflammatory dermatological condition primarily affecting the central face, including the cheeks, nose, chin, and forehead [1]. It presents with erythema, phymatous changes, papules, pustules, and telangiectasia. The pathogenesis of rosacea is still unknown. Patients often suffer from multiple symptoms of rosacea, requiring more precise assessments and individualized therapies [2]. 5-Aminolevulinic acid photodynamic therapy (ALA-PDT) has shown promise as a safe and effective treatment for rosacea [3], especially in patients with multiple subtypes. We report a successful treatment involving a 46-year-old male patient with erythematotelangiectatic rosacea and phymatous changes using trephination combined with photodynamic therapy (PDT), improving quality of life without recurrence during the 9-month follow-up period.
{"title":"Successful treatment of rosacea by trephination combined with photodynamic therapy: A case report","authors":"YunMi Qiu, Ping Yang, Xingyun Zhao, Yingzhi Luo, Liming Wu, Jianbo Zhong","doi":"10.1016/j.pdpdt.2024.104470","DOIUrl":"10.1016/j.pdpdt.2024.104470","url":null,"abstract":"<div><div>Rosacea is a chronic, inflammatory dermatological condition primarily affecting the central face, including the cheeks, nose, chin, and forehead [1]. It presents with erythema, phymatous changes, papules, pustules, and telangiectasia. The pathogenesis of rosacea is still unknown. Patients often suffer from multiple symptoms of rosacea, requiring more precise assessments and individualized therapies [2]. 5-Aminolevulinic acid photodynamic therapy (ALA-PDT) has shown promise as a safe and effective treatment for rosacea [3], especially in patients with multiple subtypes. We report a successful treatment involving a 46-year-old male patient with erythematotelangiectatic rosacea and phymatous changes using trephination combined with photodynamic therapy (PDT), improving quality of life without recurrence during the 9-month follow-up period.</div></div>","PeriodicalId":20141,"journal":{"name":"Photodiagnosis and Photodynamic Therapy","volume":"51 ","pages":"Article 104470"},"PeriodicalIF":3.1,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142928801","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2025-02-01DOI: 10.1016/j.pdpdt.2024.104438
Runqun Liu , Xiaoqing Xiang , Yanliang Li , Guozhang Ma , Junhong Ye
Verrucous carcinoma, a well-differentiated variant of squamous cell carcinoma with low-grade malignancy, is often associated with localized human papillomavirus (HPV) infection and excessive penile foreskin. We report a 45-year-old male who presented with a verrucous plaque on the glans penis for over four months, along with itching. Examination revealed a verrucous, erosive lesion with a cauliflower-like surface, firm texture, and irregular shape, accompanied by pain, bleeding, and a foul odor. Syphilis and HIV tests were negative, and a biopsy confirmed verrucous carcinoma.The patient underwent initial circumcision at a local hospital, but persistent lesions raised concerns about further surgery impairing sexual function and anatomical integrity. To preserve both anatomical and functional integrity, the patient opted for Hematoporphyrin Derivative-Photodynamic Therapy (HpD-PDT). Post-treatment, the lesions regressed, and after four years of follow-up, there was no scarring or recurrence. The clinical outcome was favorable with complete lesion regression.We propose that PDT is a viable treatment option for penile verrucous carcinoma, offering advantages over traditional methods in terms of function preservation and cosmetic outcomes.
{"title":"Photodynamic therapy with hematoporphyrin derivative for the treatment of penile verrucous carcinoma: A case report","authors":"Runqun Liu , Xiaoqing Xiang , Yanliang Li , Guozhang Ma , Junhong Ye","doi":"10.1016/j.pdpdt.2024.104438","DOIUrl":"10.1016/j.pdpdt.2024.104438","url":null,"abstract":"<div><div>Verrucous carcinoma, a well-differentiated variant of squamous cell carcinoma with low-grade malignancy, is often associated with localized human papillomavirus (HPV) infection and excessive penile foreskin. We report a 45-year-old male who presented with a verrucous plaque on the glans penis for over four months, along with itching. Examination revealed a verrucous, erosive lesion with a cauliflower-like surface, firm texture, and irregular shape, accompanied by pain, bleeding, and a foul odor. Syphilis and HIV tests were negative, and a biopsy confirmed verrucous carcinoma.The patient underwent initial circumcision at a local hospital, but persistent lesions raised concerns about further surgery impairing sexual function and anatomical integrity. To preserve both anatomical and functional integrity, the patient opted for Hematoporphyrin Derivative-Photodynamic Therapy (HpD-PDT). Post-treatment, the lesions regressed, and after four years of follow-up, there was no scarring or recurrence. The clinical outcome was favorable with complete lesion regression.We propose that PDT is a viable treatment option for penile verrucous carcinoma, offering advantages over traditional methods in terms of function preservation and cosmetic outcomes.</div></div>","PeriodicalId":20141,"journal":{"name":"Photodiagnosis and Photodynamic Therapy","volume":"51 ","pages":"Article 104438"},"PeriodicalIF":3.1,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142793080","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2025-02-01DOI: 10.1016/j.pdpdt.2024.104451
Carly Sin Ki Yeung , Sunny Chi Lik Au
{"title":"Photodiagnosis of diabetic eye disease—The Beichen Eye Study","authors":"Carly Sin Ki Yeung , Sunny Chi Lik Au","doi":"10.1016/j.pdpdt.2024.104451","DOIUrl":"10.1016/j.pdpdt.2024.104451","url":null,"abstract":"","PeriodicalId":20141,"journal":{"name":"Photodiagnosis and Photodynamic Therapy","volume":"51 ","pages":"Article 104451"},"PeriodicalIF":3.1,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142873763","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2025-02-01DOI: 10.1016/j.pdpdt.2024.104454
Kiruthika M, Malathi G
Age-related Macular Degeneration (AMD) is a leading cause of visual impairment and blindness that affects the eye from the age of fifty-five and older. It impacts on the retina, the light-sensitive layer of the eye. In early AMD, yellowish deposits called drusen, form under the retina, which could result in distortion and gradual blurring of vision. The presence of drusen is the first sign of early dry AMD. As the disease progresses, more and larger deposits develop, and blood vessels grow up from beneath the retina leading to leakage of blood, that damages the retina. In advanced AMD, peripheral vision may remain, but the straight vision is lost. Detecting AMD early is crucial, but treatments are limited, and nutritional supplements like AREDS2 formula may slow disease progression. AMD diagnosis is primarily achieved through drusen identification, a process involving fundus photography by ophthalmologists, but the early stages of AMD make this task challenging due to ambiguous drusen regions. Furthermore, the existing models have difficulty in correctly predicting the drusen regions because of the resolution of fundus images, for which a solution is proposed as a model based on deep learning. Performance can be optimized by employing both local and global knowledge when AMD issues are still in the early phases. The area of the retina where drusen forms were identified by image segmentation, and then these deposits were automatically recognized through pattern recognition techniques.
{"title":"A comprehensive review on early detection of drusen patterns in age-related macular degeneration using deep learning models","authors":"Kiruthika M, Malathi G","doi":"10.1016/j.pdpdt.2024.104454","DOIUrl":"10.1016/j.pdpdt.2024.104454","url":null,"abstract":"<div><div>Age-related Macular Degeneration (AMD) is a leading cause of visual impairment and blindness that affects the eye from the age of fifty-five and older. It impacts on the retina, the light-sensitive layer of the eye. In early AMD, yellowish deposits called drusen, form under the retina, which could result in distortion and gradual blurring of vision. The presence of drusen is the first sign of early dry AMD. As the disease progresses, more and larger deposits develop, and blood vessels grow up from beneath the retina leading to leakage of blood, that damages the retina. In advanced AMD, peripheral vision may remain, but the straight vision is lost. Detecting AMD early is crucial, but treatments are limited, and nutritional supplements like AREDS2 formula may slow disease progression. AMD diagnosis is primarily achieved through drusen identification, a process involving fundus photography by ophthalmologists, but the early stages of AMD make this task challenging due to ambiguous drusen regions. Furthermore, the existing models have difficulty in correctly predicting the drusen regions because of the resolution of fundus images, for which a solution is proposed as a model based on deep learning. Performance can be optimized by employing both local and global knowledge when AMD issues are still in the early phases. The area of the retina where drusen forms were identified by image segmentation, and then these deposits were automatically recognized through pattern recognition techniques.</div></div>","PeriodicalId":20141,"journal":{"name":"Photodiagnosis and Photodynamic Therapy","volume":"51 ","pages":"Article 104454"},"PeriodicalIF":3.1,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142883451","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2025-02-01DOI: 10.1016/j.pdpdt.2024.104460
Abdulrahman M. AlMubarak
Objective
The objective was to systematically review original studies that assessed the influence of antimicrobial photodynamic therapy (aPDT) for managing peri‑implant diseases among habitual nicotinic product (NP) users.
Methods
The research question was “Is aPDT effective for managing peri‑implant diseases among NP users?” Indexed databases (PubMed/Medline, EMBASE, Scopus, and ISI Web of Knowledge) and Google Scholar were searched up to and including December 2024 without time and language barriers. Using Boolean operators, the following keywords were searched in different combinations: antimicrobial photodynamic therapy; crestal bone loss; peri‑implant diseases; probing depth; nicotine; and smoking. Original clinical studies were included. Letters to the Editor, Case-reports, case-series, commentaries, reviews and perspectives/expert opinions were excluded. The literature search was performed in accordance with the Preferred Reporting Items for Systematic Reviews and Meta-analyses. The risk of bias (RoB) was assessed using the Cochrane RoB 2 tool.
Results
Seven RCTs performed on adult males were included and processed for data extraction. The mean ages of participants ranged between 31 and 57 years. The wavelength of the diode laser ranged between 660 and 670 nm in all studies. Results from all RCTs showed that aPDT, when performed as an adjunct to MD, is more effective in reducing peri‑implant plaque index, bleeding scores, and peri‑implant PD in contrast to when mechanical debridement (MD) was performed alone. None of the studies reported a statistically significant difference in crestal bone height, irrespective of whether MD was done with or without adjunct aPDT. In all studies, the follow-up duration ranged between 3 and 12 months. The aPDT was performed once (after MD) in all studies. All studies had a low RoB and their results were based on power-adjusted data.
Conclusion
In the short term, a single session of aPDT as an adjunct to MD effectively reduces peri‑implant soft-tissue inflammation among NP users with peri‑implant diseases. However, this treatment does not influence CBL in these patients.
目的：目的是系统地回顾评估抗菌光动力疗法（aPDT）对管理习惯性尼古丁产品（NP）使用者种植体周围疾病的影响的原始研究。方法：研究问题为“aPDT治疗NP使用者种植体周围疾病有效吗？”检索索引数据库（PubMed/Medline、EMBASE、Scopus和ISI Web of Knowledge）和谷歌Scholar，检索截止至2024年12月，没有时间和语言障碍。使用布尔运算符搜索不同组合的关键词：抗菌光动力疗法；牙冠骨质流失；高疾病;探测深度;尼古丁;和吸烟。纳入了原始临床研究。不包括致编辑的信、病例报告、病例系列、评论、评论和观点/专家意见。根据系统评价和meta分析的首选报告项目进行文献检索。使用Cochrane RoB 2工具评估偏倚风险（RoB）。结果：纳入了7项成年男性的随机对照试验，并进行了数据提取处理。参与者的平均年龄在31岁到57岁之间。在所有的研究中，二极管激光器的波长范围在660 ~ 670 nm之间。所有随机对照试验的结果都显示，与单独进行机械清创（MD）相比，aPDT作为MD的辅助手段，在降低种植体周围斑块指数、出血评分和种植体周围PD方面更有效。无论MD是否伴有辅助aPDT，没有一项研究报告在冠骨高度上有统计学上的显著差异。在所有的研究中，随访时间在3到12个月之间。在所有研究中，aPDT均在MD后进行一次。所有的研究都有较低的RoB，他们的结果是基于功率调整后的数据。结论：在短期内，单次aPDT作为MD的辅助，可以有效地减少患有种植体周围疾病的NP使用者的种植体周围软组织炎症。然而，这种治疗不影响这些患者的CBL。
{"title":"Role of antimicrobial photodynamic therapy for the management of peri‑implant diseases among habitual nicotinic product users: A systematic review","authors":"Abdulrahman M. AlMubarak","doi":"10.1016/j.pdpdt.2024.104460","DOIUrl":"10.1016/j.pdpdt.2024.104460","url":null,"abstract":"<div><h3>Objective</h3><div>The objective was to systematically review original studies that assessed the influence of antimicrobial photodynamic therapy (aPDT) for managing peri‑implant diseases among habitual nicotinic product (NP) users.</div></div><div><h3>Methods</h3><div>The research question was “Is aPDT effective for managing peri‑implant diseases among NP users?” Indexed databases (PubMed/Medline, EMBASE, Scopus, and ISI Web of Knowledge) and Google Scholar were searched up to and including December 2024 without time and language barriers. Using Boolean operators, the following keywords were searched in different combinations: antimicrobial photodynamic therapy; crestal bone loss; peri‑implant diseases; probing depth; nicotine; and smoking. Original clinical studies were included. Letters to the Editor, Case-reports, case-series, commentaries, reviews and perspectives/expert opinions were excluded. The literature search was performed in accordance with the Preferred Reporting Items for Systematic Reviews and Meta-analyses. The risk of bias (RoB) was assessed using the Cochrane RoB 2 tool.</div></div><div><h3>Results</h3><div>Seven RCTs performed on adult males were included and processed for data extraction. The mean ages of participants ranged between 31 and 57 years. The wavelength of the diode laser ranged between 660 and 670 nm in all studies. Results from all RCTs showed that aPDT, when performed as an adjunct to MD, is more effective in reducing peri‑implant plaque index, bleeding scores, and peri‑implant PD in contrast to when mechanical debridement (MD) was performed alone. None of the studies reported a statistically significant difference in crestal bone height, irrespective of whether MD was done with or without adjunct aPDT. In all studies, the follow-up duration ranged between 3 and 12 months. The aPDT was performed once (after MD) in all studies. All studies had a low RoB and their results were based on power-adjusted data.</div></div><div><h3>Conclusion</h3><div>In the short term, a single session of aPDT as an adjunct to MD effectively reduces peri‑implant soft-tissue inflammation among NP users with peri‑implant diseases. However, this treatment does not influence CBL in these patients.</div></div>","PeriodicalId":20141,"journal":{"name":"Photodiagnosis and Photodynamic Therapy","volume":"51 ","pages":"Article 104460"},"PeriodicalIF":3.1,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142908161","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Photodynamic therapy (PDT) and radiotherapy using ionizing radiation (IR) are promising options for organ-preserving treatment of bladder cancer (BCa). A combination therapy (IR+PDT) could be beneficial for BCa treatment.
Purpose
For PDT, we used the near-infrared photosensitizer tetrahydroporphyrin-tetratosylate (THPTS) showing high therapeutic efficacy. Treatment responses were analyzed in BCa organoids.
Methods
Organoids consisting of BCa cells lines, bladder fibroblasts and muscle cells were treated with IR (9 Gy) and/or PDT using THPTS (25, 50 μM; 20 J/cm2). Cytotoxicity was determined by microscopy, cell-based assays and histology. The cell death mode was analyzed by applying specific inhibitors followed by immunofluorescence or qPCR analyses of cell death markers. A matrix-based co-culture model was used to study T cell migration into the environment of treated organoids.
Results
PDT and/or IR resulted in concentration-dependent reduction of metabolic activity, organoid diameter and integrity. Higher cytotoxicity of IR+PDT vs. monotherapies was observed after 72 h. Non-malignant organoids showed no cytotoxic effects. While apoptosis, necroptosis and ferroptosis were clearly involved in cell death of T-24 cells, cytotoxicity in RT-112 cells was probably provoked by apoptosis, ferroptosis and pyroptosis. IR+PDT resulted in significant migration of Jurkat cells into ECM-embedded organoids within 3 days after treatment.
Conclusion
Treatment with IR+PDT showed tumor-selective cytotoxicity with additive or synergistic effects in BCa organoids. Thereby, IR+PDT led to multimodal cell death depending on the cellular context. Migration of T cells into the organoid environment illustrates the immunogenic potential of IR+PDT. Therefore, it might be a promising approach for organ-preserving BCa treatment.
{"title":"Ionizing radiation and photodynamic therapy lead to multimodal tumor cell death, synergistic cytotoxicity and immune cell invasion in human bladder cancer organoids","authors":"Annabell Reinhold , Annegret Glasow , Sandra Nürnberger , Annett Weimann , Lucie Telemann , Jens-Uwe Stolzenburg , Jochen Neuhaus , Mandy Berndt-Paetz","doi":"10.1016/j.pdpdt.2024.104459","DOIUrl":"10.1016/j.pdpdt.2024.104459","url":null,"abstract":"<div><h3>Background</h3><div>Photodynamic therapy (PDT) and radiotherapy using ionizing radiation (IR) are promising options for organ-preserving treatment of bladder cancer (BCa). A combination therapy (IR+PDT) could be beneficial for BCa treatment.</div></div><div><h3>Purpose</h3><div>For PDT, we used the near-infrared photosensitizer tetrahydroporphyrin-tetratosylate (THPTS) showing high therapeutic efficacy. Treatment responses were analyzed in BCa organoids.</div></div><div><h3>Methods</h3><div>Organoids consisting of BCa cells lines, bladder fibroblasts and muscle cells were treated with IR (9 Gy) and/or PDT using THPTS (25, 50 μM; 20 J/cm<sup>2</sup>). Cytotoxicity was determined by microscopy, cell-based assays and histology. The cell death mode was analyzed by applying specific inhibitors followed by immunofluorescence or qPCR analyses of cell death markers. A matrix-based co-culture model was used to study T cell migration into the environment of treated organoids.</div></div><div><h3>Results</h3><div>PDT and/or IR resulted in concentration-dependent reduction of metabolic activity, organoid diameter and integrity. Higher cytotoxicity of IR+PDT vs. monotherapies was observed after 72 h. Non-malignant organoids showed no cytotoxic effects. While apoptosis, necroptosis and ferroptosis were clearly involved in cell death of T-24 cells, cytotoxicity in RT-112 cells was probably provoked by apoptosis, ferroptosis and pyroptosis. IR+PDT resulted in significant migration of Jurkat cells into ECM-embedded organoids within 3 days after treatment.</div></div><div><h3>Conclusion</h3><div>Treatment with IR+PDT showed tumor-selective cytotoxicity with additive or synergistic effects in BCa organoids. Thereby, IR+PDT led to multimodal cell death depending on the cellular context. Migration of T cells into the organoid environment illustrates the immunogenic potential of IR+PDT. Therefore, it might be a promising approach for organ-preserving BCa treatment.</div></div>","PeriodicalId":20141,"journal":{"name":"Photodiagnosis and Photodynamic Therapy","volume":"51 ","pages":"Article 104459"},"PeriodicalIF":3.1,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142923883","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
扫码关注我们
求助内容：
应助结果提醒方式：
京公网安备 11010802042870号