Photodiagnosis and Photodynamic Therapy最新文献_第9页

Long-term outcome of photodynamic therapy with hexyl aminolevulinate, 5-aminolevulinic acid nanoemulsion and methyl aminolevulinate for low-risk Basal Cell Carcinomas 氨基乙酰丙酸己酯、5-氨基乙酰丙酸纳米乳和氨基乙酰丙酸甲酯光动力治疗低危基底细胞癌的长期疗效

IF 3.1 3区医学 Q2 ONCOLOGY

Photodiagnosis and Photodynamic Therapy

Pub Date : 2025-02-01 DOI: 10.1016/j.pdpdt.2024.104432

M. Salmivuori , M. Grönroos , T. Tani , J. Räsänen , E. Snellman , N. Neittaanmäki

Background

Non-surgical treatments are cost-effective options for low-risk basal cell carcinomas (BCCs) i.e. superficial or small nodular BCCs located outside the high-risk locations. Hexyl aminolevulinate (HAL) and 5-aminolevulinic acid nanoemulsion (BF-200 ALA) with enhanced penetration depth enables the use of lower concentrations compared to methylaminolevulinate (MAL) in photodynamic therapy (PDT). We have previously reported comparable short-term efficacies for MAL 16 %, BF-200 ALA 7.8 % and HAL 2 % in PDT of low-risk BCC, and here we report the long-term results.

Objectives

The goal of this trial was to compare long-term outcomes of HAL and BF-200 ALA, compared to MAL in PDT of low-risk BCCs.

Methods

Ninety-eight histologically verified low-risk BCCs on the trunk or extremities were included and randomized into three arms to receive PDT in two sessions with MAL, BF-200 ALA or HAL. A blinded dermatologist assessed the response, cosmetic outcome, and obtained biopsies for histological verification at three months, one year and five years. Histologically verified non-responsive lesions were excised. Patients’ satisfaction with the treatment was also queried.

Results

According to intention-to-treat (ITT) analyses, the cumulative response rate at one year was 90.6 % for MAL, 81.3 % for BF-200 ALA, and 75.8 % for HAL, and correspondingly at five years 71.9 %, 54.6 % and 60.6 %. There were no statistically significant differences between interventions and comparator. The overall cumulative response rate for PDT was 82.5 % at one year and 62.2 % at five, and 48.6 % of the treatment failures were recorded at five years. The recurrent lesions were excised as second line treatment. No aggressive subtypes were reported, with only superficial or nodular growth in the final histopathological report. There were no significant differences in cosmetic outcome or patient satisfaction.

Conclusions

This trial shows that HAL has potential for dermatological PDT. However, the long-term efficacy of PDT in the treatment of low-risk BCCs remains rather low.

背景：对于低风险基底细胞癌（bcc），即位于高危部位以外的浅表性或小结节性基底细胞癌，非手术治疗是经济有效的选择。与甲氨基乙酰丙酸（MAL）相比，具有增强渗透深度的己基氨基乙酰丙酸（HAL）和5-氨基乙酰丙酸纳米乳（BF-200 ALA）在光动力治疗（PDT）中使用的浓度更低。我们之前报道过低风险BCC PDT中MAL的短期疗效为16%，BF-200 ALA为7.8%，HAL为2%，这里我们报告的是长期结果。目的：本试验的目的是比较HAL和BF-200 ALA与MAL在低风险bcc PDT中的长期结果。方法：纳入98例经组织学证实的躯干或四肢低风险bcc，随机分为三组，分别在MAL、BF-200 ALA或HAL两组接受PDT治疗。一名盲法皮肤科医生评估了患者的反应、美容结果，并在3个月、1年和5年进行了组织学检查。组织学证实无反应的病变被切除。同时询问患者对治疗的满意度。结果：意向治疗（ITT）分析显示，1年累积缓解率MAL为90.6%，BF-200 ALA为81.3%，HAL为75.8%，5年累积缓解率分别为71.9%、54.6%和60.6%。干预组与比较组之间无统计学差异。PDT的总累积缓解率在1年为82.5%，5年为62.2%，48.6%的治疗失败记录在5年。复发病灶切除作为二线治疗。没有侵袭性亚型的报道，只有浅表或结节生长在最终的组织病理学报告。在美容效果或患者满意度方面没有显著差异。结论：本试验表明HAL有可能用于皮肤病PDT。然而，PDT治疗低风险bcc的长期疗效仍然很低。

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引用次数: 0

Comparative analysis of combined methylene blue photodynamic therapy and doxorubicin treatment of oral squamous cell carcinoma cell line: In vitro study on apoptosis 亚甲蓝光动力联合治疗与阿霉素联合治疗口腔鳞状细胞癌细胞凋亡的比较分析。

IF 3.1 3区医学 Q2 ONCOLOGY

Photodiagnosis and Photodynamic Therapy

Pub Date : 2025-02-01 DOI: 10.1016/j.pdpdt.2024.104457

Shayan Momeni Goujani (Dentist) , Maryam Koopaie , Fateme Hamta Safarian (Dentist) , Neda Hakimiha , Shima Younespour

Introduction

Squamous cell carcinoma (SCC) is the most common malignancy of the head and neck region. Combination therapy potentially enhances the effectiveness beyond that of each treatment alone. This study aimed to assess whether photodynamic therapy (PDT), using methylene blue as a photosensitizer in conjunction with doxorubicin, produces synergistic effects on the apoptosis of the oral squamous cell carcinoma (OSCC) cell line.

Materials and methods

The human oral epidermal carcinoma cell line (KB cell line, NCBI Code: C152) was cultured in Dulbecco's modified Eagle's medium. Following at least 24 hours of incubation, the OSCC cells were distributed into six groups, with groups 1–3 and 5 performed in the dark to prevent any light interference. 1: control group; 2: treated with 3.2 μg/mL methylene blue; 3: exposed to various concentrations of doxorubicin; 4: PDT group (methylene blue + 660 nm light); 5: treated with both doxorubicin and methylene blue; and finally, 6: treated with PDT (methylene blue + 660 nm light) in conjunction with doxorubicin. Flow cytometry methods were used to assess apoptosis. Analysis of variance (ANOVA) was used to compare quantitative variables between groups, and Tukey's test was applied for pairwise group comparisons.

Results

Flow cytometry analysis revealed that the highest level of cellular apoptosis occurred in the group treated with PDT in conjunction with doxorubicin.

Conclusions

PDT using the photosensitizer methylene blue, in combination with doxorubicin, can serve as an effective agent for inducing apoptosis in OSCC cells.

简介：鳞状细胞癌（SCC）是头颈部最常见的恶性肿瘤。联合治疗可能比单独治疗更有效。本研究旨在评估使用亚甲基蓝作为光敏剂与阿霉素联合使用的光动力疗法（PDT）是否对口腔鳞状细胞癌（OSCC）细胞系的凋亡产生协同作用。材料与方法：在Dulbecco改良Eagle’s培养基中培养人口腔表皮癌细胞（KB细胞系，NCBI代码：C152）。孵育至少24小时后，将OSCC细胞分为6组，1-3组和5组在黑暗中进行，以防止任何光线干扰。1：对照组；2：用3.2 μg/mL亚甲基蓝处理；3：暴露于各种浓度的阿霉素；4: PDT组（亚甲基蓝 + 660 nm光）；5：多用阿霉素和亚甲蓝治疗；最后6组：PDT（亚甲基蓝 + 660 nm光）联合阿霉素治疗。流式细胞术检测细胞凋亡。组间定量变量比较采用方差分析（ANOVA），两两组比较采用Tukey’s检验。结果：流式细胞术分析显示PDT联合阿霉素组细胞凋亡水平最高。结论：光敏剂亚甲基蓝PDT联合阿霉素可作为诱导OSCC细胞凋亡的有效药物。

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引用次数: 0

Proposal and operational evaluation of a device for external and internal photodynamic therapy treatments 一种用于外部和内部光动力治疗的装置的建议和操作评估。

IF 3.1 3区医学 Q2 ONCOLOGY

Photodiagnosis and Photodynamic Therapy

Pub Date : 2025-02-01 DOI: 10.1016/j.pdpdt.2024.104440

Enrique Navarrete de Gálvez , Pablo Fonda Pascual , José Aguilera Arjona , José Ramón de Andrés Díaz , María Navarrete de Gálvez , Shiran Perera Mohamed , María Victoria de Gálvez Aranda

Photodynamic therapy (PDT) light sources must match their emission spectrum with the absorption spectrum of the photosensitizer, provide precise treatment definition, deliver adequate irradiance, avoid thermal damage, and minimize treatment duration. Additionally, they should be adaptable to different photosensitizers, easy to manipulate, and cost-effective.

Current LED sources are difficult to customize, rigid, and primarily designed for broad-area treatments. For localized treatments, laser technology is commonly employed.

We propose a customizable and programmable LED-based device that not only meets these specifications but also addresses the limitations of current LED sources for localized treatments. It allows for the connection of a fiber optic terminal for internal treatments and can be fitted with light-diffusing devices capable of treating lesions externally or penetrating them internally. This device is an enhanced version of a previously developed source that has demonstrated efficacy in several pilot studies of photodynamic therapy.

The proposed equipment shows significant potential for both medical and research applications, enabling the configuration of emission spectra on demand and the establishment of tailored treatment protocols based on the type of lesion being treated.

光动力治疗（PDT）光源必须将其发射光谱与光敏剂的吸收光谱相匹配，提供精确的治疗定义，提供足够的辐照度以避免热损伤，并最大限度地缩短治疗时间。此外，它们应该适应不同的光敏剂，易于操作，并具有成本效益。目前的LED光源难以定制，刚性，主要用于广域处理。对于局部治疗，通常采用激光技术。我们提出了一种可定制和可编程的基于LED的设备，不仅满足这些规范，而且还解决了当前LED源在局部治疗方面的局限性。它允许连接用于内部治疗的光纤终端，并且可以安装能够从外部治疗病变或从内部穿透病变的光扩散装置。该设备是先前开发的光源的增强版本，该光源已在几项光动力治疗的试点研究中证明了有效性。拟议的设备在医疗和研究应用方面显示出巨大的潜力，能够根据需要配置发射光谱，并根据所治疗的病变类型建立量身定制的治疗方案。

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引用次数: 0

Comparison of local minocycline hydrochloride delivery and antimicrobial photodynamic therapy as adjuncts to mechanical debridement for the treatment of peri‑implant mucositis: A randomized controlled trial 比较局部米诺环素和抗菌光动力治疗作为机械清创治疗种植体周围粘膜炎的辅助手段：一项随机对照试验。

IF 3.1 3区医学 Q2 ONCOLOGY

Photodiagnosis and Photodynamic Therapy

Pub Date : 2025-02-01 DOI: 10.1016/j.pdpdt.2024.104461

Abdulrahman M. AlMubarak

Objective

The aim was to compare the efficacy of local minocycline hydrochloride delivery (LMHD) and antimicrobial photodynamic therapy (aPDT) as adjuncts to mechanical debridement (MD) for the treatment of peri‑implant mucositis (PM).

Methods

Patients with PM were included. The following information was retrieved from patients’ digital dental records: (a) age, (b) gender, (c) duration of implants in function, (d) number of implants, (e) depth of implant placement, and (f) mode of prosthesis retention. Information related to daily toothbrushing and flossing of interproximal spaces and the most recent visit to an oral healthcare provider was also recorded. Participants were randomly divided into three groups as follows: (a) MD + LMHD; (b) MD + aPDT, and (c) MD alone. Peri-implant modified plaque index (mPI), modified gingival index (mGI) and probing depth (PD) were measured at baseline and at 45 days follow-up. Peri-implant crestal bone levels were measured at baseline. Group comparisons were done using One-way analysis of variance and Bonferroni post-hoc adjustment tests. The correlation between peri‑implant clinical parameters and age, gender, and duration of implants in function was assessed using linear regression analysis. Level of significance was set at P < 0.05.

Results

Twenty-two and 22 patients underwent MD with adjunct LMDH and aPDT, respectively. Twenty-two participants underwent MD alone. There was no difference in the mean ages of all individuals. At baseline, there was no difference in mPI, mGI and PD in all groups. At follow-up, mPI (P < 0.05), mGI (P < 0.05) and PD (P < 0.05) were higher among patients who underwent MD alone than individuals who received LMHD or aPDT as adjuncts to MD. There was no difference in mPI, mGI, and PD among individuals who underwent LMHD and aPDT as adjuvants to MD.

Conclusion

In the short term, MD with adjunct LMHD or aPDT is effective for managing PM.

目的：比较盐酸米诺环素局部给药（LMHD）与抗菌光动力治疗（aPDT）辅助机械清创（MD）治疗种植体周围粘膜炎（PM）的疗效。方法：纳入PM患者。从患者的数字牙科记录中检索以下信息：(a)年龄，(b)性别，(c)种植体功能持续时间，(d)种植体数量，(e)种植体放置深度，(f)种植体保留模式。与每日刷牙和使用牙线近端间隙有关的信息以及最近一次访问口腔保健提供者的信息也被记录下来。参与者随机分为以下三组：(a) MD + LMHD；(b) MD + aPDT，和(c)单独的MD。在基线和随访45 d时测量种植体周围改良菌斑指数（mPI）、改良牙龈指数（mGI）和探探深度（PD）。在基线时测量种植体周围嵴骨水平。采用单向方差分析和Bonferroni事后调整检验进行组间比较。使用线性回归分析评估种植体周围临床参数与年龄、性别和种植体功能持续时间的相关性。结果：分别有22例和22例患者接受MD合并LMDH和aPDT。22名参与者单独接受了MD治疗。所有个体的平均年龄没有差异。在基线时，各组mPI、mGI和PD均无差异。结论：在短期内，MD联合LMHD或aPDT治疗PM有效。

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引用次数: 0

Effects of ALA-PDT on the murine footpad model of Fonsecaea monophora infection and its related mechanisms in vivo ALA-PDT对单癣菌感染小鼠足垫模型的影响及其体内机制。

IF 3.1 3区医学 Q2 ONCOLOGY

Photodiagnosis and Photodynamic Therapy

Pub Date : 2025-02-01 DOI: 10.1016/j.pdpdt.2024.104452

Xuelin Wu , Wenyi Chen , Muhammad Danish Yaqoob , Kangxing Liu , Yanqing Hu , Yan Lu , Yongxuan Hu

Background

: 5-aminolevulinic acid photodynamic therapy (ALA-PDT) has received growing attention for treating chromoblastomycosis (CBM) and has shown efficacy in a handful of clinical case reports. However, there is insufficient information regarding the effects of ALA-PDT on Fonsecaea monophora in mouse infection model and the related mechanisms. This study investigated these issues in vivo.

Methods

: A F. monophora infection mouse model inoculated in footpads was used. Changes in the footpad volume, tissue fungal burden, and histopathological characteristics were investigated to determine the efficacy of ALA-PDT. Scavenger receptor MARCO (Macrophage receptor with collagenous structure) was further evaluated at the gene and protein levels. Serum cytokines TNF-α, GM-CSF, IL-4, and IL-10 were measured using enzyme-linked immunosorbent assay to indicate changes in the immune microenvironment after PDT.

Results

: ALA-PDT reduced infected footpad volume, fungal burden, and pathological inflammatory infiltration in vivo. It also increased the expression of Marco in the murine infection model. Furthermore, PDT upregulated the anti-inflammatory cytokines IL-4 and IL-10 while downregulated the pro-inflammatory cytokines TNF-α and GM-CSF in mouse serum.

Conclusions

: ALA-PDT demonstrated fungicidal effects in a mouse footpad infection model with F. monophora and attenuated the inflammatory reactions. It may also assist against the intracellular fungi by the host through macrophage receptor MARCO and regulation of the immune microenvironment. This study provides scientific evidence for the protocol selection of ALA-PDT as a promising adjunctive modality for treating chromoblastomycosis.

背景：5-氨基乙酰丙酸光动力疗法（ALA-PDT）在治疗成色菌病（CBM）方面受到越来越多的关注，并在少数临床病例报告中显示出疗效。然而，关于ALA-PDT在小鼠感染模型中对单癣菌的作用及其机制的研究尚不充分。本研究在体内研究了这些问题。方法：采用足垫接种单念珠菌感染小鼠模型。研究了足垫体积、组织真菌负荷和组织病理学特征的变化，以确定ALA-PDT的疗效。在基因和蛋白水平上进一步评估清除者受体MARCO（巨噬细胞胶原结构受体）。采用酶联免疫吸附法检测血清细胞因子TNF-α、GM-CSF、IL-4和IL-10，以指示PDT后免疫微环境的变化。结果：ALA-PDT在体内减少了感染足垫体积、真菌负荷和病理性炎症浸润。在小鼠感染模型中也增加了Marco的表达。此外，PDT上调小鼠血清抗炎因子IL-4和IL-10，下调促炎因子TNF-α和GM-CSF。结论：ALA-PDT对小鼠足垫单孢菌感染模型具有一定的杀真菌作用，可减轻炎症反应。它也可能通过巨噬细胞受体MARCO和调节免疫微环境来帮助宿主对抗细胞内真菌。本研究为ALA-PDT作为一种有前景的辅助治疗方式的方案选择提供了科学依据。

{"title":"Effects of ALA-PDT on the murine footpad model of Fonsecaea monophora infection and its related mechanisms in vivo","authors":"Xuelin Wu , Wenyi Chen , Muhammad Danish Yaqoob , Kangxing Liu , Yanqing Hu , Yan Lu , Yongxuan Hu","doi":"10.1016/j.pdpdt.2024.104452","DOIUrl":"10.1016/j.pdpdt.2024.104452","url":null,"abstract":"<div><h3>Background</h3><div><em>:</em> 5-aminolevulinic acid photodynamic therapy (ALA-PDT) has received growing attention for treating chromoblastomycosis (CBM) and has shown efficacy in a handful of clinical case reports. However, there is insufficient information regarding the effects of ALA-PDT on <em>Fonsecaea monophora</em> in mouse infection model and the related mechanisms. This study investigated these issues <em>in vivo</em>.</div></div><div><h3>Methods</h3><div><em>:</em> A <em>F. monophora</em> infection mouse model inoculated in footpads was used. Changes in the footpad volume, tissue fungal burden, and histopathological characteristics were investigated to determine the efficacy of ALA-PDT. Scavenger receptor MARCO (Macrophage receptor with collagenous structure) was further evaluated at the gene and protein levels. Serum cytokines TNF-α, GM-CSF, IL-4, and IL-10 were measured using enzyme-linked immunosorbent assay to indicate changes in the immune microenvironment after PDT.</div></div><div><h3>Results</h3><div><em>:</em> ALA-PDT reduced infected footpad volume, fungal burden, and pathological inflammatory infiltration <em>in vivo</em>. It also increased the expression of <em>Marco</em> in the murine infection model. Furthermore, PDT upregulated the anti-inflammatory cytokines IL-4 and IL-10 while downregulated the pro-inflammatory cytokines TNF-α and GM-CSF in mouse serum.</div></div><div><h3>Conclusions</h3><div><em>:</em> ALA-PDT demonstrated fungicidal effects in a mouse footpad infection model with <em>F. monophora</em> and attenuated the inflammatory reactions. It may also assist against the intracellular fungi by the host through macrophage receptor MARCO and regulation of the immune microenvironment. This study provides scientific evidence for the protocol selection of ALA-PDT as a promising adjunctive modality for treating chromoblastomycosis.</div></div>","PeriodicalId":20141,"journal":{"name":"Photodiagnosis and Photodynamic Therapy","volume":"51 ","pages":"Article 104452"},"PeriodicalIF":3.1,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142879433","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Efficacy and safety of photodynamic therapy sequential dose-reduction concurrent chemoradiotherapy in locally advanced obstructive esophageal carcinoma: A propensity score matching analysis

IF 3.1 3区医学 Q2 ONCOLOGY

Photodiagnosis and Photodynamic Therapy

Pub Date : 2025-01-31 DOI: 10.1016/j.pdpdt.2025.104509

Ming Zhang , Zhenhua Sun , Gang Qiu , Hualin Wei , Baoshuan Fang , Ying Wang , Xiaopeng Zhang , Juan Li

Background

Dysphagia is a major symptom in esophageal carcinoma (EC) patients. photodynamic therapy (PDT) was approved for palliative treatment of patients with obstructive EC. Although it can remove the obstruction quickly, PDT may be difficult to achieve eradication alone. Thus, we aimed to assess whether photodynamic therapy sequential Dose-Reduction concurrent chemoradiotherapy (CCRT) can be an effective and safe approach for locally advanced obstructive EC.

Methods

This retrospective study included 121 patients with locally advanced obstructive EC who treated with radical CCRT (conventional treatment) and PDT sequential dose-reduction CCRT (combined treatment). A 1:1 propensity score matching (PSM) was conducted to balance potential bias. The improvement of dysphagia and overall survival (OS) was analyzed as the primary endpoint. Progression-free survival (PFS), local control, nutritional improvement and toxicities were analyzed as secondary endpoints.

Results

After PSM, 15 pairs of patients were selected for final analysis. Although the data failed to identify discrepancy in the remission rate of dysphagia between the two groups (73.3 % VS 93.3 %, P = 0.33), the degree of dysphagia relief deviated significantly (2.13 ± 0.52 VS 2.47 ± 0.52, P = 0.005). The onset of dysphagia remission was earlier in the combined treatment group than in the conventional treatment group (17.29 ± 9.29 days VS 33.73 ± 6.77 days, P < 0.001). The median OS of conventional treatment group and combined treatment group were 21.10 months (95 %CI 10.24∼31.96) and 36.67 months (95 %CI 21.54∼51.80), respectively (P = 0.048). The median PFS were 14.30 months (95 %CI 7.79∼20.81) and 31.23 months (95 %CI 13.68∼47.78), respectively (P = 0.039). The rates of 1-year, 2-year and 3-year OS of conventional treatment and combined treatment group were 60 %, 33.3 %, 20 % and 86.7 %, 66.7 %, 41.5 %, respectively. The rates of 1-year, 2-year, and 3-year PFS of the two groups were 53.3 %, 26.7 %, 13.3 % and 73.3 %, 53.3 %, 38.1 %, respectively. The improvement of nutritional status in combined treatment group was better than that in conventional treatment group. The grade 3 toxicity rate was 46.7 %, and no grade 4 or more toxicity was observed in all patients. The addition of PDT did not increase the risk of toxic reactions compared with concurrent chemoradiotherapy.

Conclusion

Photodynamic therapy sequential dose-reduction concurrent chemoradiotherapy can rapidly relieve dysphagia symptoms in patients with locally advanced obstructive EC. Compared with radical CCRT, it does not increase the incidence of treatment-related adverse reactions.

{"title":"Efficacy and safety of photodynamic therapy sequential dose-reduction concurrent chemoradiotherapy in locally advanced obstructive esophageal carcinoma: A propensity score matching analysis","authors":"Ming Zhang , Zhenhua Sun , Gang Qiu , Hualin Wei , Baoshuan Fang , Ying Wang , Xiaopeng Zhang , Juan Li","doi":"10.1016/j.pdpdt.2025.104509","DOIUrl":"10.1016/j.pdpdt.2025.104509","url":null,"abstract":"<div><h3>Background</h3><div>Dysphagia is a major symptom in esophageal carcinoma (EC) patients. photodynamic therapy (PDT) was approved for palliative treatment of patients with obstructive EC. Although it can remove the obstruction quickly, PDT may be difficult to achieve eradication alone. Thus, we aimed to assess whether photodynamic therapy sequential Dose-Reduction concurrent chemoradiotherapy (CCRT) can be an effective and safe approach for locally advanced obstructive EC.</div></div><div><h3>Methods</h3><div>This retrospective study included 121 patients with locally advanced obstructive EC who treated with radical CCRT (conventional treatment) and PDT sequential dose-reduction CCRT (combined treatment). A 1:1 propensity score matching (PSM) was conducted to balance potential bias. The improvement of dysphagia and overall survival (OS) was analyzed as the primary endpoint. Progression-free survival (PFS), local control, nutritional improvement and toxicities were analyzed as secondary endpoints.</div></div><div><h3>Results</h3><div>After PSM, 15 pairs of patients were selected for final analysis. Although the data failed to identify discrepancy in the remission rate of dysphagia between the two groups (73.3 % VS 93.3 %, <em>P</em> = 0.33), the degree of dysphagia relief deviated significantly (2.13 ± 0.52 VS 2.47 ± 0.52, <em>P</em> = 0.005). The onset of dysphagia remission was earlier in the combined treatment group than in the conventional treatment group (17.29 ± 9.29 days VS 33.73 ± 6.77 days, <em>P</em> < 0.001). The median OS of conventional treatment group and combined treatment group were 21.10 months (95 %CI 10.24∼31.96) and 36.67 months (95 %CI 21.54∼51.80), respectively (<em>P</em> = 0.048). The median PFS were 14.30 months (95 %CI 7.79∼20.81) and 31.23 months (95 %CI 13.68∼47.78), respectively (<em>P</em> = 0.039). The rates of 1-year, 2-year and 3-year OS of conventional treatment and combined treatment group were 60 %, 33.3 %, 20 % and 86.7 %, 66.7 %, 41.5 %, respectively. The rates of 1-year, 2-year, and 3-year PFS of the two groups were 53.3 %, 26.7 %, 13.3 % and 73.3 %, 53.3 %, 38.1 %, respectively. The improvement of nutritional status in combined treatment group was better than that in conventional treatment group. The grade 3 toxicity rate was 46.7 %, and no grade 4 or more toxicity was observed in all patients. The addition of PDT did not increase the risk of toxic reactions compared with concurrent chemoradiotherapy.</div></div><div><h3>Conclusion</h3><div>Photodynamic therapy sequential dose-reduction concurrent chemoradiotherapy can rapidly relieve dysphagia symptoms in patients with locally advanced obstructive EC. Compared with radical CCRT, it does not increase the incidence of treatment-related adverse reactions.</div></div>","PeriodicalId":20141,"journal":{"name":"Photodiagnosis and Photodynamic Therapy","volume":"52 ","pages":"Article 104509"},"PeriodicalIF":3.1,"publicationDate":"2025-01-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143082454","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Photodynamic therapy combined with palliative resection and secondary healing for periocular basal cell carcinoma with inadequate surgical margins

IF 3.1 3区医学 Q2 ONCOLOGY

Photodiagnosis and Photodynamic Therapy

Pub Date : 2025-01-31 DOI: 10.1016/j.pdpdt.2025.104500

Xiaoqing Wang , Ting Zhang , Tianhang Li , Yahui Feng , Li Ma , Dongmei Li , Yeqiang Song , Dongmei Shi

Periocular basal cell carcinoma (pBCC) is the most common malignancy in the periocular region, characterized by local aggressiveness, high incidence, and significant recurrence rates. While surgery is the primary treatment option, achieving complete lesion removal can be challenging due to the sensitive anatomy of this area and associated cosmetic considerations. Recent advancements in photodynamic therapy (PDT) have demonstrated promising efficacy and cosmetic outcomes for superficial BCC, offering alternatives to traditional treatments. However, PDT's effectiveness is often limited by the penetration depth of photosensitizers, particularly for deep tumors. To address these challenges, we developed a regimen that combines palliative surgery with immediate PDT for pBCC cases with inadequate or positive surgical margins. This integrated approach aims to overcome the penetration limitations of PDT while enhancing tumor clearance and preserving cosmetic outcomes. We report on three cases where all patients achieved complete clinical remission, with satisfactory cosmetic results and no recurrence observed during follow-up periods ranging from 12 to 23 months. Our findings suggest that palliative surgical resection, in conjunction with PDT, represents an effective treatment strategy for pBCC. This approach is particularly valuable when standard surgical margins cannot be achieved, especially considering the importance of cosmetic healing.

{"title":"Photodynamic therapy combined with palliative resection and secondary healing for periocular basal cell carcinoma with inadequate surgical margins","authors":"Xiaoqing Wang , Ting Zhang , Tianhang Li , Yahui Feng , Li Ma , Dongmei Li , Yeqiang Song , Dongmei Shi","doi":"10.1016/j.pdpdt.2025.104500","DOIUrl":"10.1016/j.pdpdt.2025.104500","url":null,"abstract":"<div><div>Periocular basal cell carcinoma (pBCC) is the most common malignancy in the periocular region, characterized by local aggressiveness, high incidence, and significant recurrence rates. While surgery is the primary treatment option, achieving complete lesion removal can be challenging due to the sensitive anatomy of this area and associated cosmetic considerations. Recent advancements in photodynamic therapy (PDT) have demonstrated promising efficacy and cosmetic outcomes for superficial BCC, offering alternatives to traditional treatments. However, PDT's effectiveness is often limited by the penetration depth of photosensitizers, particularly for deep tumors. To address these challenges, we developed a regimen that combines palliative surgery with immediate PDT for pBCC cases with inadequate or positive surgical margins. This integrated approach aims to overcome the penetration limitations of PDT while enhancing tumor clearance and preserving cosmetic outcomes. We report on three cases where all patients achieved complete clinical remission, with satisfactory cosmetic results and no recurrence observed during follow-up periods ranging from 12 to 23 months. Our findings suggest that palliative surgical resection, in conjunction with PDT, represents an effective treatment strategy for pBCC. This approach is particularly valuable when standard surgical margins cannot be achieved, especially considering the importance of cosmetic healing.</div></div>","PeriodicalId":20141,"journal":{"name":"Photodiagnosis and Photodynamic Therapy","volume":"52 ","pages":"Article 104500"},"PeriodicalIF":3.1,"publicationDate":"2025-01-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143082520","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The evaluation of the macular and optic nerve head microvasculature in amblyopic and non-amblyopic patients with constant exotropia in comparison to healthy controls

IF 3.1 3区医学 Q2 ONCOLOGY

Photodiagnosis and Photodynamic Therapy

Pub Date : 2025-01-30 DOI: 10.1016/j.pdpdt.2025.104506

Vahideh Manouchehri , Amir Eftekhari Milani , Raheleh Chegini , Amin Arasteh

Objective

To evaluate the retinal microvascular changes in strabismic amblyopia and compare them to the strabismic cases without amblyopia and controls.

Design

Cross-sectional study.

Participants

Three groups of patients, including 25 amblyopic cases with exotropia (XT), 25 constant XT cases without amblyopia, and 25 healthy subjects as controls, are included in the study. Both eyes of the included participants are evaluated.

Methods

All the patients underwent a thorough ophthalmic examination, BCVA assessment, and macular and optic nerve head OCTA with the AngioVue™ Imaging System.

Main Outcome Measures

The vascular density (VD) of various layers (Deep and superficial capillary plexus (DCP and SCP) in different regions of the macula, optic nerve head, and FAZ area were evaluated as the target variables.

Results

42 females and 33 males with a mean age of 32.2 (95%CI:29.8-34.6) entered our study. The median exotropia in both groups was 35 PD. The parafoveal DCP VD was significantly lower in both eyes of amblyopic cases than in controls (53.56 and 52.31 vs. 56.13%, p:0.021, <0.001). The parafoveal SCP VD was only lower in the dominant eye of the amblyopic cases than in the controls (50.53 vs. 53.54%, p:0.002). The foveal and peripapillary VD showed no significant difference between the groups. The FAZ area was significantly larger in amblyopic eyes than the fellow eyes (0.250 vs. 0.194 mm², p:0.025) and correlated with worse BCVA (Correlation Coefficient: 0.358, p: 0.011).

Conclusions

The amblyopia could be accompanied by a lower parafoveal DCP VD and a larger FAZ area in strabismic amblyopia.

{"title":"The evaluation of the macular and optic nerve head microvasculature in amblyopic and non-amblyopic patients with constant exotropia in comparison to healthy controls","authors":"Vahideh Manouchehri , Amir Eftekhari Milani , Raheleh Chegini , Amin Arasteh","doi":"10.1016/j.pdpdt.2025.104506","DOIUrl":"10.1016/j.pdpdt.2025.104506","url":null,"abstract":"<div><h3>Objective</h3><div>To evaluate the retinal microvascular changes in strabismic amblyopia and compare them to the strabismic cases without amblyopia and controls.</div></div><div><h3>Design</h3><div>Cross-sectional study.</div></div><div><h3>Participants</h3><div>Three groups of patients, including 25 amblyopic cases with exotropia (XT), 25 constant XT cases without amblyopia, and 25 healthy subjects as controls, are included in the study. Both eyes of the included participants are evaluated.</div></div><div><h3>Methods</h3><div>All the patients underwent a thorough ophthalmic examination, BCVA assessment, and macular and optic nerve head OCTA with the AngioVue™ Imaging System.</div></div><div><h3>Main Outcome Measures</h3><div>The vascular density (VD) of various layers (Deep and superficial capillary plexus (DCP and SCP) in different regions of the macula, optic nerve head, and FAZ area were evaluated as the target variables.</div></div><div><h3>Results</h3><div>42 females and 33 males with a mean age of 32.2 (95%CI:29.8-34.6) entered our study. The median exotropia in both groups was 35 PD. The parafoveal DCP VD was significantly lower in both eyes of amblyopic cases than in controls (53.56 and 52.31 vs. 56.13%, p:0.021, <0.001). The parafoveal SCP VD was only lower in the dominant eye of the amblyopic cases than in the controls (50.53 vs. 53.54%, p:0.002). The foveal and peripapillary VD showed no significant difference between the groups. The FAZ area was significantly larger in amblyopic eyes than the fellow eyes (0.250 vs. 0.194 mm<sup>2</sup>, p:0.025) and correlated with worse BCVA (Correlation Coefficient: 0.358, p: 0.011).</div></div><div><h3>Conclusions</h3><div>The amblyopia could be accompanied by a lower parafoveal DCP VD and a larger FAZ area in strabismic amblyopia.</div></div>","PeriodicalId":20141,"journal":{"name":"Photodiagnosis and Photodynamic Therapy","volume":"52 ","pages":"Article 104506"},"PeriodicalIF":3.1,"publicationDate":"2025-01-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143075709","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Salivary attenuated total reflectance-fourier transform infrared spectroscopy combined with chemometric analysis: A potential point-of-care approach for chronic kidney disease screening

IF 3.1 3区医学 Q2 ONCOLOGY

Photodiagnosis and Photodynamic Therapy

Pub Date : 2025-01-30 DOI: 10.1016/j.pdpdt.2025.104502

Kamonchanok Tangwanichgapong , Poramaporn Klanrit , Patutong Chatchawal , Molin Wongwattanakul , Cholatip Pongskul , Rajda Chaichit , Doosadee Hormdee

Background

The increasing prevalence of chronic kidney disease (CKD) and its terminal stage, end-stage renal disease (ESRD), raises the importance of an accurate, early, and point-of-care method to diagnose and monitor patients. Saliva is a potential point-of-care diagnostic biofluid for its simple collection and ability to reflect systemic health status. This study investigated salivary spectral signatures in ESRD patients and their diagnostic potential compared to healthy controls.

Methods

Saliva samples were collected from 24 ESRD patients undergoing hemodialysis and 24 age/sex-matched healthy controls. The dried saliva samples were analyzed using Attenuated Total Reflectance-Fourier Transform Infrared (ATR-FTIR) spectroscopy in the 4000–400 cm⁻¹ range. Chemometric analyses, including Principal Component Analysis (PCA) and Partial Least Squares Discriminant Analysis (PLS-DA), were applied to preprocessed spectra to identify discriminatory spectral features and establish classification models.

Results

Second derivative spectroscopic analysis of ATR-FTIR spectra revealed distinctive spectral patterns in dried ESRD saliva samples, including characteristic peak shifts observed in both the amide I secondary structures (from 1636 cm^-1 in controls to 1629 cm^-1 in ESRD) and carbohydrate (from 1037 cm^-1 in controls to 1042 cm^-1 in ESRD) regions. PCA demonstrated clear clustering patterns across key biological spectral regions, including the lipid CH stretching region (3000–2800 cm⁻¹), the fingerprint region (1800–900 cm⁻¹), and their combination (3000–2800 cm⁻¹ + 1800–900 cm⁻¹). PLS models based on the fingerprint region achieved optimal diagnostic performance (87.5–100 % accuracy, 75–100 % sensitivity, and 100 % specificity). Biochemical markers associated with ESRD revealed variations in lipids, protein, sugar moieties, carbohydrates, and nucleic acids, reflecting the underlying pathological changes in CKD, with the most prominent band at ∼1405 cm^-1.

Conclusion

ATR-FTIR analysis of dried saliva demonstrated potential as a non-invasive diagnostic tool for ESRD. This approach could complement existing diagnostic methods, particularly in resource-limited settings or for frequent monitoring requirements.

{"title":"Salivary attenuated total reflectance-fourier transform infrared spectroscopy combined with chemometric analysis: A potential point-of-care approach for chronic kidney disease screening","authors":"Kamonchanok Tangwanichgapong , Poramaporn Klanrit , Patutong Chatchawal , Molin Wongwattanakul , Cholatip Pongskul , Rajda Chaichit , Doosadee Hormdee","doi":"10.1016/j.pdpdt.2025.104502","DOIUrl":"10.1016/j.pdpdt.2025.104502","url":null,"abstract":"<div><h3>Background</h3><div>The increasing prevalence of chronic kidney disease (CKD) and its terminal stage, end-stage renal disease (ESRD), raises the importance of an accurate, early, and point-of-care method to diagnose and monitor patients. Saliva is a potential point-of-care diagnostic biofluid for its simple collection and ability to reflect systemic health status. This study investigated salivary spectral signatures in ESRD patients and their diagnostic potential compared to healthy controls.</div></div><div><h3>Methods</h3><div>Saliva samples were collected from 24 ESRD patients undergoing hemodialysis and 24 age/sex-matched healthy controls. The dried saliva samples were analyzed using Attenuated Total Reflectance-Fourier Transform Infrared (ATR-FTIR) spectroscopy in the 4000–400 cm⁻¹ range. Chemometric analyses, including Principal Component Analysis (PCA) and Partial Least Squares Discriminant Analysis (PLS-DA), were applied to preprocessed spectra to identify discriminatory spectral features and establish classification models.</div></div><div><h3>Results</h3><div>Second derivative spectroscopic analysis of ATR-FTIR spectra revealed distinctive spectral patterns in dried ESRD saliva samples, including characteristic peak shifts observed in both the amide I secondary structures (from 1636 cm<sup>-1</sup> in controls to 1629 cm<sup>-1</sup> in ESRD) and carbohydrate (from 1037 cm<sup>-1</sup> in controls to 1042 cm<sup>-1</sup> in ESRD) regions. PCA demonstrated clear clustering patterns across key biological spectral regions, including the lipid CH stretching region (3000–2800 cm<sup>−1</sup>), the fingerprint region (1800–900 cm<sup>−1</sup>), and their combination (3000–2800 cm<sup>−1</sup> + 1800–900 cm<sup>−1</sup>). PLS models based on the fingerprint region achieved optimal diagnostic performance (87.5–100 % accuracy, 75–100 % sensitivity, and 100 % specificity). Biochemical markers associated with ESRD revealed variations in lipids, protein, sugar moieties, carbohydrates, and nucleic acids, reflecting the underlying pathological changes in CKD, with the most prominent band at ∼1405 cm<sup>-1</sup>.</div></div><div><h3>Conclusion</h3><div>ATR-FTIR analysis of dried saliva demonstrated potential as a non-invasive diagnostic tool for ESRD. This approach could complement existing diagnostic methods, particularly in resource-limited settings or for frequent monitoring requirements.</div></div>","PeriodicalId":20141,"journal":{"name":"Photodiagnosis and Photodynamic Therapy","volume":"52 ","pages":"Article 104502"},"PeriodicalIF":3.1,"publicationDate":"2025-01-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143076824","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Impact of repeated low-level red-light therapy on axial length, refraction, and macular retinal blood flow density in adolescents with mild to moderate myopia

IF 3.1 3区医学 Q2 ONCOLOGY

Photodiagnosis and Photodynamic Therapy

Pub Date : 2025-01-30 DOI: 10.1016/j.pdpdt.2025.104499

Meifeng Ji , Teer Ba , Huixia Li , Dongxue Wang , Guisen Zhang , Wei Wang

Background

In order to evaluate the effectiveness of repeated low-level red-light (RLRL) therapy on stabilizing axial elongation and refractive changes in adolescents with mild to moderate myopia. In addition, we also examined whether RLRL therapy affects retinal blood flow density in the macular region, a factor previously unstudied in this context.

Methods

Conducted at a single clinical site, this retrospective, single-arm study followed participants over six months, with assessments at baseline, 1 month, 3 months, and 6 months. The primary outcomes included axial length and spherical equivalent refraction (SER) changes. Secondary assessments included retinal blood flow density (superficial and deep macular layers), white-to-white corneal diameter, central corneal thickness, intraocular pressure (IOP), corneal curvature, light sensitivity, and peripheral retinal thickness.

Results

There were 32 enrolled participants (mean age = 11.5 ± 1.72) in the current study. The spherical equivalent (SER) remained relatively stable over the first three months, but it significantly improved at six months, changing from -2.39 ± 2.21 D at baseline to -2.01 ± 2.12 D (P < 0.05). However, the axial length also showed minimal variation across follow-up visits, indicating stable eye growth with no substantial elongation throughout the study period. Furthermore, RLRL therapy resulted in stable measurements across primary and secondary outcomes (all P > 0.05), with no significant changes over the six months. There were no obvious side effects following the treatment.

Conclusions

Low-energy red light therapy shows promise as a non-invasive approach for stabilizing myopia in adolescents, with no observed compromise to retinal blood flow density. Further longitudinal research is needed to validate these findings and support clinical recommendations for myopia management.

{"title":"Impact of repeated low-level red-light therapy on axial length, refraction, and macular retinal blood flow density in adolescents with mild to moderate myopia","authors":"Meifeng Ji , Teer Ba , Huixia Li , Dongxue Wang , Guisen Zhang , Wei Wang","doi":"10.1016/j.pdpdt.2025.104499","DOIUrl":"10.1016/j.pdpdt.2025.104499","url":null,"abstract":"<div><h3>Background</h3><div>In order to evaluate the effectiveness of repeated low-level red-light (RLRL) therapy on stabilizing axial elongation and refractive changes in adolescents with mild to moderate myopia. In addition, we also examined whether RLRL therapy affects retinal blood flow density in the macular region, a factor previously unstudied in this context.</div></div><div><h3>Methods</h3><div>Conducted at a single clinical site, this retrospective, single-arm study followed participants over six months, with assessments at baseline, 1 month, 3 months, and 6 months. The primary outcomes included axial length and spherical equivalent refraction (SER) changes. Secondary assessments included retinal blood flow density (superficial and deep macular layers), white-to-white corneal diameter, central corneal thickness, intraocular pressure (IOP), corneal curvature, light sensitivity, and peripheral retinal thickness.</div></div><div><h3>Results</h3><div>There were 32 enrolled participants (mean age = 11.5 ± 1.72) in the current study. The spherical equivalent (SER) remained relatively stable over the first three months, but it significantly improved at six months, changing from -2.39 ± 2.21 D at baseline to -2.01 ± 2.12 D (<em>P</em> < 0.05). However, the axial length also showed minimal variation across follow-up visits, indicating stable eye growth with no substantial elongation throughout the study period. Furthermore, RLRL therapy resulted in stable measurements across primary and secondary outcomes (all <em>P</em> > 0.05), with no significant changes over the six months. There were no obvious side effects following the treatment.</div></div><div><h3>Conclusions</h3><div>Low-energy red light therapy shows promise as a non-invasive approach for stabilizing myopia in adolescents, with no observed compromise to retinal blood flow density. Further longitudinal research is needed to validate these findings and support clinical recommendations for myopia management.</div></div>","PeriodicalId":20141,"journal":{"name":"Photodiagnosis and Photodynamic Therapy","volume":"52 ","pages":"Article 104499"},"PeriodicalIF":3.1,"publicationDate":"2025-01-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143076822","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

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