首页 > 最新文献

Physiologia plantarum最新文献

英文 中文
Gibberellic acid and light effects on seed germination in the seagrass Zostera marina.
IF 5.4 2区 生物学 Q1 PLANT SCIENCES Pub Date : 2025-03-01 DOI: 10.1111/ppl.70137
Riccardo Pieraccini, Lawrence Whatley, Nico Koedam, Ann Vanreusel, Tobias Dolch, Jasper Dierick, Tom Van der Stocken

Seagrass meadows have been heavily affected by human activities, with Zostera marina L. (Zosteraceae) being one of the most impacted species. Seed-based methods are currently the preferred approach for their restoration, yet low germination rates and poor seedling establishment remain significant challenges. This study explored the combined effects of light spectra (white, red, and darkness), photoperiod, and gibberellic acid (GA3-0, 50, 500, and 1000 mg L-1) on Z. marina seed germination using a fully crossed incubation experiment. Penalised logistic regression and Cox proportional hazards analysis were chosen to account for low germination events and to analyse the temporal dynamics of germination. We found that light conditions, particularly red light and darkness, when combined with GA3, significantly enhanced germination probability. Furthermore, mid (50 mg L-1) and high (500 mg L-1) GA3 concentrations reduced time-to-germination. Morphometric analysis of the cotyledonary and leaf tissue development indicates no adverse effects of the treatments on seedling development. Our findings suggest that light and GA3 treatments effectively improve germination success and reduce dormancy in Z. marina seeds. Seed treatments can mitigate stress- or manipulation-induced dormancy and can represent a viable strategy for on-demand germination, such as in the context of seed-based restoration efforts.

{"title":"Gibberellic acid and light effects on seed germination in the seagrass Zostera marina.","authors":"Riccardo Pieraccini, Lawrence Whatley, Nico Koedam, Ann Vanreusel, Tobias Dolch, Jasper Dierick, Tom Van der Stocken","doi":"10.1111/ppl.70137","DOIUrl":"10.1111/ppl.70137","url":null,"abstract":"<p><p>Seagrass meadows have been heavily affected by human activities, with Zostera marina L. (Zosteraceae) being one of the most impacted species. Seed-based methods are currently the preferred approach for their restoration, yet low germination rates and poor seedling establishment remain significant challenges. This study explored the combined effects of light spectra (white, red, and darkness), photoperiod, and gibberellic acid (GA<sub>3-</sub>0, 50, 500, and 1000 mg L<sup>-1</sup>) on Z. marina seed germination using a fully crossed incubation experiment. Penalised logistic regression and Cox proportional hazards analysis were chosen to account for low germination events and to analyse the temporal dynamics of germination. We found that light conditions, particularly red light and darkness, when combined with GA<sub>3</sub>, significantly enhanced germination probability. Furthermore, mid (50 mg L<sup>-1</sup>) and high (500 mg L<sup>-1</sup>) GA<sub>3</sub> concentrations reduced time-to-germination. Morphometric analysis of the cotyledonary and leaf tissue development indicates no adverse effects of the treatments on seedling development. Our findings suggest that light and GA<sub>3</sub> treatments effectively improve germination success and reduce dormancy in Z. marina seeds. Seed treatments can mitigate stress- or manipulation-induced dormancy and can represent a viable strategy for on-demand germination, such as in the context of seed-based restoration efforts.</p>","PeriodicalId":20164,"journal":{"name":"Physiologia plantarum","volume":"177 2","pages":"e70137"},"PeriodicalIF":5.4,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11894247/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143597625","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Differential gene expression and metabolic pathways in Toona sinensis: Influence on colour and aroma.
IF 5.4 2区 生物学 Q1 PLANT SCIENCES Pub Date : 2025-03-01 DOI: 10.1111/ppl.70146
Rui Zeng, Mingmin Zheng, Yunhong Gao, Jianchun Hu, Javed Ahmad, Muhammad Umer Farooq, Songqing Liu, Xiangmei Lin, Suleyman I Allakhverdiev, Sergey Shabala

Toona sinensis, a plant species renowned for its culinary and medicinal properties, exhibits diverse colour variations that contribute to its aesthetic appeal and commercial value. Understanding the molecular mechanisms underlying colour and aroma traits in Toona sinensis is crucial for breeding programs and quality regulation in agriculture and the food industry. The present investigation included a comprehensive analysis of the transcriptomic and metabolomic profiles of Toona sinensis with different colours, including green, red, and red leaves with green stems. Metabolic analysis revealed that the flavonoid biosynthesis pathway governs the colour distinction between green and red Toona sinensis. The top 10 metabolites influenced by transcriptome include terpenoids (5), heterocyclic compounds (1), phenol (1), ketone (1), aldehyde (1), and alcohol (1). Fifteen highly expressed genes impacted by phenylpropanoid, sesquiterpenoid, and triterpenoid biosynthesis in coloured Toona sinensis. Functional annotation and pathway analysis revealed that terpene metabolites are predominantly synthesized via terpene metabolic pathway, involving eight key gene families. This study underscores the importance of multi-omics approaches in unravelling the genetic and metabolic basis of phenotypic traits in plant species aimed at improving colour, aroma, and nutritional quality in plants and derived products. HIGHLIGHTS: Flavonoid biosynthesis pathway governs the colour distinction between green and red Toona sinensis. The top 10 metabolites influenced by transcriptome include five terpenoids, one heterocyclic compound, one phenol, one ketone, one aldehyde, and one alcohol. Fifteen highly expressed genes impacted by phenylpropanoid, sesquiterpenoid, and triterpenoid biosynthesis in coloured Toona sinensis. Terpene metabolites are predominantly synthesized via the terpene metabolic pathway, involving eight key gene families. The net photosynthetic rate and intercellular CO2 concentration are relatively high in the red Toon sinensis morph.

{"title":"Differential gene expression and metabolic pathways in Toona sinensis: Influence on colour and aroma.","authors":"Rui Zeng, Mingmin Zheng, Yunhong Gao, Jianchun Hu, Javed Ahmad, Muhammad Umer Farooq, Songqing Liu, Xiangmei Lin, Suleyman I Allakhverdiev, Sergey Shabala","doi":"10.1111/ppl.70146","DOIUrl":"10.1111/ppl.70146","url":null,"abstract":"<p><p>Toona sinensis, a plant species renowned for its culinary and medicinal properties, exhibits diverse colour variations that contribute to its aesthetic appeal and commercial value. Understanding the molecular mechanisms underlying colour and aroma traits in Toona sinensis is crucial for breeding programs and quality regulation in agriculture and the food industry. The present investigation included a comprehensive analysis of the transcriptomic and metabolomic profiles of Toona sinensis with different colours, including green, red, and red leaves with green stems. Metabolic analysis revealed that the flavonoid biosynthesis pathway governs the colour distinction between green and red Toona sinensis. The top 10 metabolites influenced by transcriptome include terpenoids (5), heterocyclic compounds (1), phenol (1), ketone (1), aldehyde (1), and alcohol (1). Fifteen highly expressed genes impacted by phenylpropanoid, sesquiterpenoid, and triterpenoid biosynthesis in coloured Toona sinensis. Functional annotation and pathway analysis revealed that terpene metabolites are predominantly synthesized via terpene metabolic pathway, involving eight key gene families. This study underscores the importance of multi-omics approaches in unravelling the genetic and metabolic basis of phenotypic traits in plant species aimed at improving colour, aroma, and nutritional quality in plants and derived products. HIGHLIGHTS: Flavonoid biosynthesis pathway governs the colour distinction between green and red Toona sinensis. The top 10 metabolites influenced by transcriptome include five terpenoids, one heterocyclic compound, one phenol, one ketone, one aldehyde, and one alcohol. Fifteen highly expressed genes impacted by phenylpropanoid, sesquiterpenoid, and triterpenoid biosynthesis in coloured Toona sinensis. Terpene metabolites are predominantly synthesized via the terpene metabolic pathway, involving eight key gene families. The net photosynthetic rate and intercellular CO<sub>2</sub> concentration are relatively high in the red Toon sinensis morph.</p>","PeriodicalId":20164,"journal":{"name":"Physiologia plantarum","volume":"177 2","pages":"e70146"},"PeriodicalIF":5.4,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11896931/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143606185","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
StZIP2 promotes root growth by improving the transport efficiency of zinc in potato(Solanum tuberosum L.).
IF 5.4 2区 生物学 Q1 PLANT SCIENCES Pub Date : 2025-03-01 DOI: 10.1111/ppl.70153
Junliang Fan, Cunlan Zhu, Xingru Si, Wenjin Xu, Liang Yang, Kaitong Wang, Ning Zhang, Huaijun Si

Zinc is an essential trace element for plant growth and development. Zinc transporters play an important role in regulating zinc homeostasis in plants. In this study, the potato cultivar 'Atlantic' was used as experimental material to analyze the expression characteristics of the StZIP2 gene in different potato tissues under zinc deficiency stress. Transgenic plants with overexpression and interference expression of the StZIP2 gene were obtained by genetic transformation and treated with zinc deficiency stress. Chlorophyll content, antioxidant enzyme activity, proline (Pro) and malonic dialdehyde (MDA) content, zinc content in aboveground parts and roots, and root indices were determined. The results showed that the expression level of the StZIP2 gene in roots, stems and leaves under zinc deficiency stress was significantly higher than that of the control, and the expression level of the StZIP2 gene in roots under zinc deficiency stress was the highest. After zinc deficiency treatment, the content of chlorophyll and Pro, the activity of catalase (CAT), peroxidase (POD), superoxide dismutase (SOD), root-to-shoot ratio, root length and root fresh weight of overexpressed plants were significantly increased, while the MDA content was significantly decreased. The ratio of zinc content between the above-ground part and the root of the overexpressed plants was significantly higher than that of the non-transgenic plants, and the transport efficiency from the root to the above-ground part was significantly increased in the overexpressed plants, whereas it was just the opposite in the interference expressing plants. The result provides basic data to further elucidate the StZIP2 gene function.

{"title":"StZIP2 promotes root growth by improving the transport efficiency of zinc in potato(Solanum tuberosum L.).","authors":"Junliang Fan, Cunlan Zhu, Xingru Si, Wenjin Xu, Liang Yang, Kaitong Wang, Ning Zhang, Huaijun Si","doi":"10.1111/ppl.70153","DOIUrl":"https://doi.org/10.1111/ppl.70153","url":null,"abstract":"<p><p>Zinc is an essential trace element for plant growth and development. Zinc transporters play an important role in regulating zinc homeostasis in plants. In this study, the potato cultivar 'Atlantic' was used as experimental material to analyze the expression characteristics of the StZIP2 gene in different potato tissues under zinc deficiency stress. Transgenic plants with overexpression and interference expression of the StZIP2 gene were obtained by genetic transformation and treated with zinc deficiency stress. Chlorophyll content, antioxidant enzyme activity, proline (Pro) and malonic dialdehyde (MDA) content, zinc content in aboveground parts and roots, and root indices were determined. The results showed that the expression level of the StZIP2 gene in roots, stems and leaves under zinc deficiency stress was significantly higher than that of the control, and the expression level of the StZIP2 gene in roots under zinc deficiency stress was the highest. After zinc deficiency treatment, the content of chlorophyll and Pro, the activity of catalase (CAT), peroxidase (POD), superoxide dismutase (SOD), root-to-shoot ratio, root length and root fresh weight of overexpressed plants were significantly increased, while the MDA content was significantly decreased. The ratio of zinc content between the above-ground part and the root of the overexpressed plants was significantly higher than that of the non-transgenic plants, and the transport efficiency from the root to the above-ground part was significantly increased in the overexpressed plants, whereas it was just the opposite in the interference expressing plants. The result provides basic data to further elucidate the StZIP2 gene function.</p>","PeriodicalId":20164,"journal":{"name":"Physiologia plantarum","volume":"177 2","pages":"e70153"},"PeriodicalIF":5.4,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143616753","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Alleviation of salt stress in strawberries by hydrogen-rich water: Physiological, transcriptomic and metabolomic responses.
IF 5.4 2区 生物学 Q1 PLANT SCIENCES Pub Date : 2025-03-01 DOI: 10.1111/ppl.70151
Renyuan Wang, Shaohua Chu, Dan Zhang, Kashif Hayat, Xia Zhang, Yaowei Chi, Xianzhong Ma, Xunfeng Chen, Haiyan Yang, Wenjiang Ding, Ting Zhao, Yongfeng Ren, Xijia Yang, Pei Zhou

The increasing impacts of climate change and intensified human activities exacerbate soil salinization, posing significant challenges to agricultural productivity. Therefore, addressing salt stress in crops is a critical area of research. In this study, strawberry seedlings (Fragaria×ananassa Duch. 'Benihoppe') were used to investigate the alleviating effects of hydrogen-rich water (HRW) on salt stress through integrated transcriptomic and metabolomic analyses. HRW treatment was found to significantly enhance plant growth, notably increasing root biomass by 49.50%. Additionally, HRW modulated key parameters, including the levels of soluble sugars, malondialdehyde (MDA), and antioxidant enzyme activities, while promoting K+ uptake and Na+ exclusion. Transcriptomic analysis revealed that HRW induced the expression of genes associated with ion transport, antioxidant defence, and cell wall biosynthesis in roots. Metabolomic profiling identified phenolic acids, flavonoids, and amino acids as critical metabolites in HRW-mediated salt stress mitigation. Integrated multi-omics analysis highlighted two key metabolic pathways, phenylpropanoid biosynthesis and amino and nucleoside sugar metabolism, pivotal to the observed protective effects. This study provides molecular insights into the mechanisms by which HRW alleviates salt stress in strawberry seedlings, underscoring the potential of hydrogen gas applications in sustainable agriculture.

{"title":"Alleviation of salt stress in strawberries by hydrogen-rich water: Physiological, transcriptomic and metabolomic responses.","authors":"Renyuan Wang, Shaohua Chu, Dan Zhang, Kashif Hayat, Xia Zhang, Yaowei Chi, Xianzhong Ma, Xunfeng Chen, Haiyan Yang, Wenjiang Ding, Ting Zhao, Yongfeng Ren, Xijia Yang, Pei Zhou","doi":"10.1111/ppl.70151","DOIUrl":"https://doi.org/10.1111/ppl.70151","url":null,"abstract":"<p><p>The increasing impacts of climate change and intensified human activities exacerbate soil salinization, posing significant challenges to agricultural productivity. Therefore, addressing salt stress in crops is a critical area of research. In this study, strawberry seedlings (Fragaria×ananassa Duch. 'Benihoppe') were used to investigate the alleviating effects of hydrogen-rich water (HRW) on salt stress through integrated transcriptomic and metabolomic analyses. HRW treatment was found to significantly enhance plant growth, notably increasing root biomass by 49.50%. Additionally, HRW modulated key parameters, including the levels of soluble sugars, malondialdehyde (MDA), and antioxidant enzyme activities, while promoting K<sup>+</sup> uptake and Na<sup>+</sup> exclusion. Transcriptomic analysis revealed that HRW induced the expression of genes associated with ion transport, antioxidant defence, and cell wall biosynthesis in roots. Metabolomic profiling identified phenolic acids, flavonoids, and amino acids as critical metabolites in HRW-mediated salt stress mitigation. Integrated multi-omics analysis highlighted two key metabolic pathways, phenylpropanoid biosynthesis and amino and nucleoside sugar metabolism, pivotal to the observed protective effects. This study provides molecular insights into the mechanisms by which HRW alleviates salt stress in strawberry seedlings, underscoring the potential of hydrogen gas applications in sustainable agriculture.</p>","PeriodicalId":20164,"journal":{"name":"Physiologia plantarum","volume":"177 2","pages":"e70151"},"PeriodicalIF":5.4,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143606184","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Blue or far-red light supplementation induced pre-hardening in the leaves of the Rht12 wheat dwarfing line: hormonal changes and freezing tolerance.
IF 5.4 2区 生物学 Q1 PLANT SCIENCES Pub Date : 2025-03-01 DOI: 10.1111/ppl.70112
Zsolt Gulyás, Mohamed Ahres, Tamás Pálmai, Kitti Kulman, Zahra Tahmasebi, Kalpita Singh, Kristóf Jobbágy, Danuše Tarkowská, Petre Dobrev, Radomíra Vanková, Péter Borbély, Andreas Börner, Gábor Galiba

Reduced height (Rht) genes have revolutionised wheat cultivation, but they can compromise freezing tolerance, and only a few alleles are in use. Thus, evaluating the role of other Rht alleles in stress responses is crucial. Far-red supplementation of white light (W+FR) can induce pre-hardening in cereals at 15°C. However, the relevant effect of blue light enrichment (W+B) is poorly described. This study investigates the influence of W+FR or W+B exposure in young winter wheat leaves of a tall (wild-type, rht12) and a dwarf, gibberellin-deficient (near-isogenic line, Rht12) genotype in cv. Maris Huntsman background over 10 days at 15°C. The main objectives were to investigate the relationship between light quality, gibberellin homeostasis, and freezing tolerance. Key parameters such as frost injury, hormonal pools and the expression of relevant genes were examined. Results provided evidence about the involvement of Rht alleles in the basal freezing tolerance of wheat leaves from the side of gibberellin availability. It was revealed that W+FR and W+B treatments partially rescued the freezing-sensitive phenotype of Rht12 leaves, suggesting a potential compensatory mechanism. Analysis of gibberellic acid (GA) metabolism indicated differential responses to light treatments between the Rht12 and wild-type leaves, with implications for freezing tolerance. Moreover, alterations in hormone levels, including jasmonic acid (JA) and salicylic acid (SA), were observed, highlighting the complex interplay between light signalling and hormonal regulation in wheat. Overall, these findings suggest that manipulating light responses may offer a strategy to enhance freezing tolerance in gibberellin-deficient dwarf wheat genotypes.

{"title":"Blue or far-red light supplementation induced pre-hardening in the leaves of the Rht12 wheat dwarfing line: hormonal changes and freezing tolerance.","authors":"Zsolt Gulyás, Mohamed Ahres, Tamás Pálmai, Kitti Kulman, Zahra Tahmasebi, Kalpita Singh, Kristóf Jobbágy, Danuše Tarkowská, Petre Dobrev, Radomíra Vanková, Péter Borbély, Andreas Börner, Gábor Galiba","doi":"10.1111/ppl.70112","DOIUrl":"10.1111/ppl.70112","url":null,"abstract":"<p><p>Reduced height (Rht) genes have revolutionised wheat cultivation, but they can compromise freezing tolerance, and only a few alleles are in use. Thus, evaluating the role of other Rht alleles in stress responses is crucial. Far-red supplementation of white light (W+FR) can induce pre-hardening in cereals at 15°C. However, the relevant effect of blue light enrichment (W+B) is poorly described. This study investigates the influence of W+FR or W+B exposure in young winter wheat leaves of a tall (wild-type, rht12) and a dwarf, gibberellin-deficient (near-isogenic line, Rht12) genotype in cv. Maris Huntsman background over 10 days at 15°C. The main objectives were to investigate the relationship between light quality, gibberellin homeostasis, and freezing tolerance. Key parameters such as frost injury, hormonal pools and the expression of relevant genes were examined. Results provided evidence about the involvement of Rht alleles in the basal freezing tolerance of wheat leaves from the side of gibberellin availability. It was revealed that W+FR and W+B treatments partially rescued the freezing-sensitive phenotype of Rht12 leaves, suggesting a potential compensatory mechanism. Analysis of gibberellic acid (GA) metabolism indicated differential responses to light treatments between the Rht12 and wild-type leaves, with implications for freezing tolerance. Moreover, alterations in hormone levels, including jasmonic acid (JA) and salicylic acid (SA), were observed, highlighting the complex interplay between light signalling and hormonal regulation in wheat. Overall, these findings suggest that manipulating light responses may offer a strategy to enhance freezing tolerance in gibberellin-deficient dwarf wheat genotypes.</p>","PeriodicalId":20164,"journal":{"name":"Physiologia plantarum","volume":"177 2","pages":"e70112"},"PeriodicalIF":5.4,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11876090/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143542966","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Correction to "Impact of water stress to plant epigenetic mechanisms in stress and adaptation".
IF 5.4 2区 生物学 Q1 PLANT SCIENCES Pub Date : 2025-03-01 DOI: 10.1111/ppl.70140
{"title":"Correction to \"Impact of water stress to plant epigenetic mechanisms in stress and adaptation\".","authors":"","doi":"10.1111/ppl.70140","DOIUrl":"https://doi.org/10.1111/ppl.70140","url":null,"abstract":"","PeriodicalId":20164,"journal":{"name":"Physiologia plantarum","volume":"177 2","pages":"e70140"},"PeriodicalIF":5.4,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143542968","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Transgenic Cynodon dactylon overexpressing CdPIF4 alters plant development and cold stress tolerance. 过表达CdPIF4的转基因Cynodon dactylon改变了植物的发育和对冷胁迫的耐受性。
IF 5.4 2区 生物学 Q1 PLANT SCIENCES Pub Date : 2025-01-01 DOI: 10.1111/ppl.70025
Xiao Xu, Xiaoyan Liu, Yanling Yin, Shugao Fan, Yunjie Qi, Yiquan Xing, Jinmin Fu

Bermudagrass [Cynodon dactylon (L.) Pers.] is widely used for soil remediation, livestock forage, and as turfgrass for sports fields, parks, and gardens due to its resilience and adaptability. However, low temperatures are critical factors limiting its geographical distribution and ornamental season, even preventing its safe overwintering. PHYTOCHROME-INTERACTING FACTOR 4 (PIF4) acts as a hub transcription factor, not only regulating various light responses but also integrating multiple external stimuli to improve plant productivity and architectural adaptation under adverse stress conditions, which makes it potential as a target gene. In this study, we cloned and characterized the CdPIF4 genes in bermudagrass. Expression analysis revealed that it is predominantly expressed in leaves and is regulated by photoperiod and cold stress. Using Agrobacterium-mediated genetic modification, we successfully generated CdPIF4a-overexpressing bermudagrass lines. Under cold stress at 4°C, these transgenic plants demonstrated enhanced cold tolerance, as indicated by higher relative water content, reduced membrane damage, and lower levels of lipid peroxidation levels. Photosynthetic analysis revealed that CdPIF4a-overexpressing plants exhibited higher light energy capture and transfer efficiency at this low temperature, with less energy loss. Additionally, they showed higher antioxidant enzyme activity and lower levels of reactive oxygen species levels. The responsive regulation of cold stress-related genes further validated the role of the CdPIF4a gene in enhancing cold tolerance. This study elucidates that CdPIF4 enhances cold tolerance in bermudagrass through physiological and molecular mechanisms, offering new insights and valuable genetic resources for advancing cold resistance research in bermudagrass and other grass species.

百慕大草[长爪龙]珀耳斯。由于其弹性和适应性,被广泛用于土壤修复、牲畜饲料、运动场、公园和花园的草坪草。然而,低温是限制其地理分布和观赏季节的关键因素,甚至阻碍了其安全越冬。PHYTOCHROME-INTERACTING FACTOR 4 (PIF4)作为中枢转录因子,不仅能调控各种光响应,还能整合多种外界刺激,提高植物在逆境条件下的生产力和建筑适应性,这使其成为潜在的靶基因。在本研究中,我们克隆并鉴定了百慕大草的CdPIF4基因。表达分析表明,该基因主要在叶片中表达,受光周期和冷胁迫的调控。利用农杆菌介导的基因修饰,我们成功地获得了过表达cdpif4a的百慕大草品系。在4°C的低温胁迫下,这些转基因植株表现出更高的相对含水量、更少的膜损伤和更低的脂质过氧化水平,表现出更强的耐寒性。光合分析表明,cdpif4a过表达植物在低温下表现出更高的光能捕获和转移效率,能量损失更少。此外,它们还表现出较高的抗氧化酶活性和较低的活性氧水平。冷应激相关基因的响应性调控进一步验证了CdPIF4a基因在增强耐寒性中的作用。本研究阐明了CdPIF4基因通过生理和分子机制增强了百慕大草的抗寒性,为推进百慕大草和其他禾本科植物的抗寒性研究提供了新的见解和宝贵的遗传资源。
{"title":"Transgenic Cynodon dactylon overexpressing CdPIF4 alters plant development and cold stress tolerance.","authors":"Xiao Xu, Xiaoyan Liu, Yanling Yin, Shugao Fan, Yunjie Qi, Yiquan Xing, Jinmin Fu","doi":"10.1111/ppl.70025","DOIUrl":"https://doi.org/10.1111/ppl.70025","url":null,"abstract":"<p><p>Bermudagrass [Cynodon dactylon (L.) Pers.] is widely used for soil remediation, livestock forage, and as turfgrass for sports fields, parks, and gardens due to its resilience and adaptability. However, low temperatures are critical factors limiting its geographical distribution and ornamental season, even preventing its safe overwintering. PHYTOCHROME-INTERACTING FACTOR 4 (PIF4) acts as a hub transcription factor, not only regulating various light responses but also integrating multiple external stimuli to improve plant productivity and architectural adaptation under adverse stress conditions, which makes it potential as a target gene. In this study, we cloned and characterized the CdPIF4 genes in bermudagrass. Expression analysis revealed that it is predominantly expressed in leaves and is regulated by photoperiod and cold stress. Using Agrobacterium-mediated genetic modification, we successfully generated CdPIF4a-overexpressing bermudagrass lines. Under cold stress at 4°C, these transgenic plants demonstrated enhanced cold tolerance, as indicated by higher relative water content, reduced membrane damage, and lower levels of lipid peroxidation levels. Photosynthetic analysis revealed that CdPIF4a-overexpressing plants exhibited higher light energy capture and transfer efficiency at this low temperature, with less energy loss. Additionally, they showed higher antioxidant enzyme activity and lower levels of reactive oxygen species levels. The responsive regulation of cold stress-related genes further validated the role of the CdPIF4a gene in enhancing cold tolerance. This study elucidates that CdPIF4 enhances cold tolerance in bermudagrass through physiological and molecular mechanisms, offering new insights and valuable genetic resources for advancing cold resistance research in bermudagrass and other grass species.</p>","PeriodicalId":20164,"journal":{"name":"Physiologia plantarum","volume":"177 1","pages":"e70025"},"PeriodicalIF":5.4,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142915255","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
The molecular mechanism of transforming red light signal to (E)-β-caryophyllene biosynthesis in Arabidopsis. 拟南芥将红光信号转化为(E)-β-石竹烯生物合成的分子机制
IF 5.4 2区 生物学 Q1 PLANT SCIENCES Pub Date : 2025-01-01 DOI: 10.1111/ppl.70065
Chuanjia Xu, Xin Wang, Malakkhanim Mehraliyeva, Jia Sun, Fangfang Chen, Changfu Li, Zhengqin Xu, Nan Tang, Yansheng Zhang

It is known that red light irradiation enhances the biosynthesis of (E)-β-caryophyllene in plants. However, the underlying mechanism connecting red light to (E)-β-caryophyllene biosynthesis remains elusive. This study reveals a molecular cascade involving the phyB-PIF4-MYC2 module, which regulates (E)-β-caryophyllene biosynthesis in response to the red light signal in Arabidopsis thaliana. In this module, phyB positively regulates (E)-β-caryophyllene biosynthesis under red light, whereas PIF4 negatively regulates it; both regulations require the involvement of MYC2, a transcription factor that can bind directly to the promoter of the TPS21 gene which encodes (E)-β-caryophyllene synthase. Importantly, protein-protein and protein-DNA interaction assays show that PIF4 reduces the binding affinity of MYC2 to the TPS21 promoter through direct interaction with MYC2. We propose that the phyB-PIF4-MYC2 module represents a universal mechanism linking red light to sesquiterpene biosynthesis in plants.

众所周知,红光照射可促进植物体内(E)-β-石竹烯的生物合成。然而,将红光与(E)-β-石竹烯生物合成联系起来的潜在机制尚不清楚。本研究揭示了拟南芥(Arabidopsis thaliana)响应红光信号调控(E)-β-石竹烯生物合成的一个涉及phyB-PIF4-MYC2模块的分子级联。在本模块中,红光下phyB正调控(E)-β-石竹烯的生物合成,而PIF4负调控;这两种调节都需要MYC2的参与,MYC2是一种转录因子,可以直接结合编码(E)-β-石蜡烯合成酶的TPS21基因的启动子。重要的是,蛋白质-蛋白质和蛋白质- dna相互作用实验表明,PIF4通过与MYC2的直接相互作用降低了MYC2与TPS21启动子的结合亲和力。我们认为phyB-PIF4-MYC2模块代表了将红光与植物中倍半萜生物合成联系起来的普遍机制。
{"title":"The molecular mechanism of transforming red light signal to (E)-β-caryophyllene biosynthesis in Arabidopsis.","authors":"Chuanjia Xu, Xin Wang, Malakkhanim Mehraliyeva, Jia Sun, Fangfang Chen, Changfu Li, Zhengqin Xu, Nan Tang, Yansheng Zhang","doi":"10.1111/ppl.70065","DOIUrl":"https://doi.org/10.1111/ppl.70065","url":null,"abstract":"<p><p>It is known that red light irradiation enhances the biosynthesis of (E)-β-caryophyllene in plants. However, the underlying mechanism connecting red light to (E)-β-caryophyllene biosynthesis remains elusive. This study reveals a molecular cascade involving the phyB-PIF4-MYC2 module, which regulates (E)-β-caryophyllene biosynthesis in response to the red light signal in Arabidopsis thaliana. In this module, phyB positively regulates (E)-β-caryophyllene biosynthesis under red light, whereas PIF4 negatively regulates it; both regulations require the involvement of MYC2, a transcription factor that can bind directly to the promoter of the TPS21 gene which encodes (E)-β-caryophyllene synthase. Importantly, protein-protein and protein-DNA interaction assays show that PIF4 reduces the binding affinity of MYC2 to the TPS21 promoter through direct interaction with MYC2. We propose that the phyB-PIF4-MYC2 module represents a universal mechanism linking red light to sesquiterpene biosynthesis in plants.</p>","PeriodicalId":20164,"journal":{"name":"Physiologia plantarum","volume":"177 1","pages":"e70065"},"PeriodicalIF":5.4,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143010085","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
A NAC transcription factor NAC50 regulates Fe reutilization in Arabidopsis under Fe-deficient condition.
IF 5.4 2区 生物学 Q1 PLANT SCIENCES Pub Date : 2025-01-01 DOI: 10.1111/ppl.70047
Jing Huang, Chun Yan Tu, Hao Yu Wang, Qiang Zhang, Ren Fang Shen, Lu Zheng, Xiao Fang Zhu

A lack of iron (Fe) inhibits the growth and development of plants, leading to reduced agricultural yields and quality. In the last ten years, numerous studies have focused on the induction of Fe uptake and translocation under Fe deficiency, but the regulatory mechanisms governing Fe reutilization within plants are still not well understood. Here, we demonstrated the involvement of the NAM/ATAF1/2/CUC2 (NAC) transcription factor NAC50 in response to Fe shortage. The content of soluble Fe was greatly reduced in nac50 mutants, leading to increased chlorosis in the newly emerging leaves under the Fe-deficient condition. Subsequent investigation revealed that the cell wall of the nac50 mutants' roots accumulated more Fe, along with an increment in hemicellulose content, indicating that a cell wall-associated Fe reutilization pathway was involved in the NAC50-regulated Fe insufficiency response. Interestingly, the expression of NINE-CIS-EPOXYCAROTENOID DIOXYGENASE 3 (NCED3), a key enzyme in the abscisic acid (ABA) biosynthetic pathway, was down-regulated in the Fe-deficient nac50 mutants, resulting in decreased endogenous ABA level and Fe-deficient sensitive phenotype. Since no direct relationship was observed between NAC50 and NCED3, this suggests a potential role of NAC50 in mediating the ABA accumulation. Moreover, exogenous ABA application in the nac50 mutant restored Fe deficiency resistance to the level observed in wild-type plants (WT), indicating that NAC50 induced the cell wall Fe reutilization potentially through the regulation of ABA accumulation.

{"title":"A NAC transcription factor NAC50 regulates Fe reutilization in Arabidopsis under Fe-deficient condition.","authors":"Jing Huang, Chun Yan Tu, Hao Yu Wang, Qiang Zhang, Ren Fang Shen, Lu Zheng, Xiao Fang Zhu","doi":"10.1111/ppl.70047","DOIUrl":"https://doi.org/10.1111/ppl.70047","url":null,"abstract":"<p><p>A lack of iron (Fe) inhibits the growth and development of plants, leading to reduced agricultural yields and quality. In the last ten years, numerous studies have focused on the induction of Fe uptake and translocation under Fe deficiency, but the regulatory mechanisms governing Fe reutilization within plants are still not well understood. Here, we demonstrated the involvement of the NAM/ATAF1/2/CUC2 (NAC) transcription factor NAC50 in response to Fe shortage. The content of soluble Fe was greatly reduced in nac50 mutants, leading to increased chlorosis in the newly emerging leaves under the Fe-deficient condition. Subsequent investigation revealed that the cell wall of the nac50 mutants' roots accumulated more Fe, along with an increment in hemicellulose content, indicating that a cell wall-associated Fe reutilization pathway was involved in the NAC50-regulated Fe insufficiency response. Interestingly, the expression of NINE-CIS-EPOXYCAROTENOID DIOXYGENASE 3 (NCED3), a key enzyme in the abscisic acid (ABA) biosynthetic pathway, was down-regulated in the Fe-deficient nac50 mutants, resulting in decreased endogenous ABA level and Fe-deficient sensitive phenotype. Since no direct relationship was observed between NAC50 and NCED3, this suggests a potential role of NAC50 in mediating the ABA accumulation. Moreover, exogenous ABA application in the nac50 mutant restored Fe deficiency resistance to the level observed in wild-type plants (WT), indicating that NAC50 induced the cell wall Fe reutilization potentially through the regulation of ABA accumulation.</p>","PeriodicalId":20164,"journal":{"name":"Physiologia plantarum","volume":"177 1","pages":"e70047"},"PeriodicalIF":5.4,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143060176","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Plants as biofactories for production of the aphid sex pheromone nepetalactone.
IF 5.4 2区 生物学 Q1 PLANT SCIENCES Pub Date : 2025-01-01 DOI: 10.1111/ppl.70110
Abraham Ontiveros-Cisneros, Jule Salfeld, Bao-Jian Ding, Hong-Lei Wang, Oliver Moss, Magne Friberg, Alex Van Moerkercke, Christer Löfstedt, Olivier Van Aken

Aphids cause massive agricultural losses through direct damage or as pathogen vectors. Control often relies on insecticides, which are expensive and not selective. An interesting alternative is to use aphid sex pheromones nepetalactone (NON) and nepetalactol (NOL) to interfere with aphid mating or attract aphid predators. Here, we explore production of these compounds in plants, as their precursors can be derived from mevalonate (MVA) and methylerythritol phosphate (MEP) pathways. By introducing six genes, including a major latex protein-like (MLPL) enzyme, we engineered a functional nepetalactol biosynthetic pathway into plants. Transient expression of these enzymes in N. benthamiana caused production of nepetalactone, without the need for external supplementation with substrates. Targeting all six enzymes into the chloroplast appeared to result in higher NON yields than just chloroplast-targeting the first two enzymes. We could not detect NOL, suggesting it is rapidly oxidised to NON. In addition, we produced NON in stably transformed Camelina sativa (Camelina) lines. Interestingly, the specific NON enantiomer was different in N. benthamiana compared to in Camelina, indicating the value of different platforms for producing specific isoforms. This opens possibilities for using plants as green factories of pheromones for baits or as pheromone dispensers that interfere with insect behaviour.

{"title":"Plants as biofactories for production of the aphid sex pheromone nepetalactone.","authors":"Abraham Ontiveros-Cisneros, Jule Salfeld, Bao-Jian Ding, Hong-Lei Wang, Oliver Moss, Magne Friberg, Alex Van Moerkercke, Christer Löfstedt, Olivier Van Aken","doi":"10.1111/ppl.70110","DOIUrl":"10.1111/ppl.70110","url":null,"abstract":"<p><p>Aphids cause massive agricultural losses through direct damage or as pathogen vectors. Control often relies on insecticides, which are expensive and not selective. An interesting alternative is to use aphid sex pheromones nepetalactone (NON) and nepetalactol (NOL) to interfere with aphid mating or attract aphid predators. Here, we explore production of these compounds in plants, as their precursors can be derived from mevalonate (MVA) and methylerythritol phosphate (MEP) pathways. By introducing six genes, including a major latex protein-like (MLPL) enzyme, we engineered a functional nepetalactol biosynthetic pathway into plants. Transient expression of these enzymes in N. benthamiana caused production of nepetalactone, without the need for external supplementation with substrates. Targeting all six enzymes into the chloroplast appeared to result in higher NON yields than just chloroplast-targeting the first two enzymes. We could not detect NOL, suggesting it is rapidly oxidised to NON. In addition, we produced NON in stably transformed Camelina sativa (Camelina) lines. Interestingly, the specific NON enantiomer was different in N. benthamiana compared to in Camelina, indicating the value of different platforms for producing specific isoforms. This opens possibilities for using plants as green factories of pheromones for baits or as pheromone dispensers that interfere with insect behaviour.</p>","PeriodicalId":20164,"journal":{"name":"Physiologia plantarum","volume":"177 1","pages":"e70110"},"PeriodicalIF":5.4,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11830648/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143433628","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
期刊
Physiologia plantarum
全部 Acc. Chem. Res. ACS Applied Bio Materials ACS Appl. Electron. Mater. ACS Appl. Energy Mater. ACS Appl. Mater. Interfaces ACS Appl. Nano Mater. ACS Appl. Polym. Mater. ACS BIOMATER-SCI ENG ACS Catal. ACS Cent. Sci. ACS Chem. Biol. ACS Chemical Health & Safety ACS Chem. Neurosci. ACS Comb. Sci. ACS Earth Space Chem. ACS Energy Lett. ACS Infect. Dis. ACS Macro Lett. ACS Mater. Lett. ACS Med. Chem. Lett. ACS Nano ACS Omega ACS Photonics ACS Sens. ACS Sustainable Chem. Eng. ACS Synth. Biol. Anal. Chem. BIOCHEMISTRY-US Bioconjugate Chem. BIOMACROMOLECULES Chem. Res. Toxicol. Chem. Rev. Chem. Mater. CRYST GROWTH DES ENERG FUEL Environ. Sci. Technol. Environ. Sci. Technol. Lett. Eur. J. Inorg. Chem. IND ENG CHEM RES Inorg. Chem. J. Agric. Food. Chem. J. Chem. Eng. Data J. Chem. Educ. J. Chem. Inf. Model. J. Chem. Theory Comput. J. Med. Chem. J. Nat. Prod. J PROTEOME RES J. Am. Chem. Soc. LANGMUIR MACROMOLECULES Mol. Pharmaceutics Nano Lett. Org. Lett. ORG PROCESS RES DEV ORGANOMETALLICS J. Org. Chem. J. Phys. Chem. J. Phys. Chem. A J. Phys. Chem. B J. Phys. Chem. C J. Phys. Chem. Lett. Analyst Anal. Methods Biomater. Sci. Catal. Sci. Technol. Chem. Commun. Chem. Soc. Rev. CHEM EDUC RES PRACT CRYSTENGCOMM Dalton Trans. Energy Environ. Sci. ENVIRON SCI-NANO ENVIRON SCI-PROC IMP ENVIRON SCI-WAT RES Faraday Discuss. Food Funct. Green Chem. Inorg. Chem. Front. Integr. Biol. J. Anal. At. Spectrom. J. Mater. Chem. A J. Mater. Chem. B J. Mater. Chem. C Lab Chip Mater. Chem. Front. Mater. Horiz. MEDCHEMCOMM Metallomics Mol. Biosyst. Mol. Syst. Des. Eng. Nanoscale Nanoscale Horiz. Nat. Prod. Rep. New J. Chem. Org. Biomol. Chem. Org. Chem. Front. PHOTOCH PHOTOBIO SCI PCCP Polym. Chem.
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
0
微信
客服QQ
Book学术公众号 扫码关注我们
反馈
×
意见反馈
请填写您的意见或建议
请填写您的手机或邮箱
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
现在去查看 取消
×
提示
确定
Book学术官方微信
Book学术文献互助
Book学术文献互助群
群 号:481959085
Book学术
文献互助 智能选刊 最新文献 互助须知 联系我们:info@booksci.cn
Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。
Copyright © 2023 Book学术 All rights reserved.
ghs 京公网安备 11010802042870号 京ICP备2023020795号-1