Abiotic stresses significantly impact agricultural productivity and food security. Innovative strategies, including the use of plant-derived compounds and plant growth-promoting rhizobacteria (PGPR), are necessary to enhance plant resilience. This study delved into how Bacillus zanthoxyli HS1 (BzaHS1) and BzaHS1-derived volatile organic compounds (VOC) conferred systemic tolerance against salt and heat stresses in cabbage and cucumber plants. Direct application of a BzaHS1 strain or exposure of BzaHS1-derived VOC to cabbage and cucumber plants promoted seedling growth under stressed conditions. This induced systemic tolerance was associated with increased mRNA expression and enzymatic activities of superoxide dismutase (EC 1.15.1.1), catalase (EC 1.11.1.6), or ascorbate peroxidase (EC 1.11.1.1), leading to a reduction in oxidative stress in cabbage and cucumber plants. Plants co-cultured with BzaHS1 and exposed to BzaHS1-derived VOC triggered the accumulation of callose and minimized stomatal opening in response to high salt and temperature stresses, respectively. In contrast, exogenous treatment of azelaic acid, a well-characterized plant defense primer, had no significant impact on the seedling growth of cabbage and cucumber plants grown under abiotic stress conditions. Taken together, BzaHS1 and its VOC show potential for enhancing plant tolerance responses to salt and heat stresses through modulation of osmotic stress-regulatory networks.
{"title":"Insights into Bacillus zanthoxyli HS1-mediated systemic tolerance: multifunctional implications for enhanced plant tolerance to abiotic stresses.","authors":"Anahita Barghi, Ho Won Jung","doi":"10.1111/ppl.14458","DOIUrl":"https://doi.org/10.1111/ppl.14458","url":null,"abstract":"<p><p>Abiotic stresses significantly impact agricultural productivity and food security. Innovative strategies, including the use of plant-derived compounds and plant growth-promoting rhizobacteria (PGPR), are necessary to enhance plant resilience. This study delved into how Bacillus zanthoxyli HS1 (BzaHS1) and BzaHS1-derived volatile organic compounds (VOC) conferred systemic tolerance against salt and heat stresses in cabbage and cucumber plants. Direct application of a BzaHS1 strain or exposure of BzaHS1-derived VOC to cabbage and cucumber plants promoted seedling growth under stressed conditions. This induced systemic tolerance was associated with increased mRNA expression and enzymatic activities of superoxide dismutase (EC 1.15.1.1), catalase (EC 1.11.1.6), or ascorbate peroxidase (EC 1.11.1.1), leading to a reduction in oxidative stress in cabbage and cucumber plants. Plants co-cultured with BzaHS1 and exposed to BzaHS1-derived VOC triggered the accumulation of callose and minimized stomatal opening in response to high salt and temperature stresses, respectively. In contrast, exogenous treatment of azelaic acid, a well-characterized plant defense primer, had no significant impact on the seedling growth of cabbage and cucumber plants grown under abiotic stress conditions. Taken together, BzaHS1 and its VOC show potential for enhancing plant tolerance responses to salt and heat stresses through modulation of osmotic stress-regulatory networks.</p>","PeriodicalId":20164,"journal":{"name":"Physiologia plantarum","volume":null,"pages":null},"PeriodicalIF":5.4,"publicationDate":"2024-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141894010","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Gábor Patyi, Barbara Hódi, Ivy Mallick, Gergely Maróti, Péter B Kós, Imre Vass
Singlet oxygen (1O2) is an important reactive oxygen species whose formation by the type-II, light-dependent, photodynamic reaction is inevitable during photosynthetic processes. In the last decades, the recognition that 1O2 is not only a damaging agent, but can also affect gene expression and participates in signal transduction pathways has received increasing attention. However, contrary to several other taxa, 1O2-responsive genes have not been identified in the important cyanobacterial model organism Synechocystis PCC 6803. By using global transcript analysis we have identified a large set of Synechocystis genes, whose transcript levels were either enhanced or repressed in the presence of 1O2. Characteristic 1O2 responses were observed in several light-inducible genes of Synechocystis, especially in the hli (or scp) family encoding HLIP/SCP proteins involved in photoprotection. Other important 1O2-induced genes include components of the Photosystem II repair machinery (psbA2 and ftsH2, ftsH3), iron homeostasis genes isiA and idiA, the group 2 sigma factor sigD, some components of the transcriptomes induced by salt-, hyperosmotic and cold-stress, as well as several genes of unknown function. The most pronounced 1O2-induced upregulation was observed for the hliB and the co-transcribed lilA genes, whose deletion induced enhanced sensitivity against 1O2-mediated light damage. A bioreporter Synechocystis strain was created by fusing the hliB promoter to the bacterial luciferase (lux), which showed its utility for continuous monitoring of 1O2 concentrations inside the cell.
{"title":"Investigation of singlet-oxygen-responsive genes in the cyanobacterium Synechocystis PCC 6803.","authors":"Gábor Patyi, Barbara Hódi, Ivy Mallick, Gergely Maróti, Péter B Kós, Imre Vass","doi":"10.1111/ppl.14468","DOIUrl":"https://doi.org/10.1111/ppl.14468","url":null,"abstract":"<p><p>Singlet oxygen (<sup>1</sup>O<sub>2</sub>) is an important reactive oxygen species whose formation by the type-II, light-dependent, photodynamic reaction is inevitable during photosynthetic processes. In the last decades, the recognition that <sup>1</sup>O<sub>2</sub> is not only a damaging agent, but can also affect gene expression and participates in signal transduction pathways has received increasing attention. However, contrary to several other taxa, <sup>1</sup>O<sub>2</sub>-responsive genes have not been identified in the important cyanobacterial model organism Synechocystis PCC 6803. By using global transcript analysis we have identified a large set of Synechocystis genes, whose transcript levels were either enhanced or repressed in the presence of <sup>1</sup>O<sub>2</sub>. Characteristic <sup>1</sup>O<sub>2</sub> responses were observed in several light-inducible genes of Synechocystis, especially in the hli (or scp) family encoding HLIP/SCP proteins involved in photoprotection. Other important <sup>1</sup>O<sub>2</sub>-induced genes include components of the Photosystem II repair machinery (psbA2 and ftsH2, ftsH3), iron homeostasis genes isiA and idiA, the group 2 sigma factor sigD, some components of the transcriptomes induced by salt-, hyperosmotic and cold-stress, as well as several genes of unknown function. The most pronounced <sup>1</sup>O<sub>2</sub>-induced upregulation was observed for the hliB and the co-transcribed lilA genes, whose deletion induced enhanced sensitivity against <sup>1</sup>O<sub>2</sub>-mediated light damage. A bioreporter Synechocystis strain was created by fusing the hliB promoter to the bacterial luciferase (lux), which showed its utility for continuous monitoring of <sup>1</sup>O<sub>2</sub> concentrations inside the cell.</p>","PeriodicalId":20164,"journal":{"name":"Physiologia plantarum","volume":null,"pages":null},"PeriodicalIF":5.4,"publicationDate":"2024-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141976360","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Ambreen Gul, Abdul Qayyum Rao, Mukhtar Ahmed, Ayesha Latif, Allah Bakhsh, Sehrish Iftikhar
The whitefly, a polyphagous insect pest feeding on nearly 1328 plant species, is a major threat to global cotton production and incurs up to 50% yield losses in cotton production in Pakistan. We investigated whether increased aspartate in phloem sap imparts whitefly toxicity and protects cotton plants from intense damage. The enzymatic step for aspartate production is carried through aspartate aminotransferase (AAT). In this study, we constitutively overexpressed the Oryza sativa cytoplasmic AAT (OsAAT2) under the CaMV35S promoter in Gossypium hirsutum cv. CIM-482. Real-time PCR analysis of the AAT transcripts revealed a 2.85- to 31.7-fold increase in mRNA levels between the different cotton lines. A substantial increase in the free-amino acid content of the major N-assimilation and transport amino acids (aspartate, glutamate, asparagine, and glutamine) was seen in the phloem sap of the transgenic cotton lines. The bioassay revealed that the two transgenic cotton lines with the highest free aspartate content in the phloem sap exhibited 97 and 94% mortality in the adult whitefly population and a 98 and 96% decline in subsequent nymph populations, respectively. There was also a significant change in the physiological behaviour of the transgenic cotton lines, with an increased net assimilation (A), gaseous exchange (Gs) and rate of transpiration (E). Improved morphological characteristics like plant height, total number of bolls and fiber yield were recorded in transgenic cotton lines. The AAT gene shows promise in mitigating whitefly infestations and enhancing the overall health and yield of cotton plants.
{"title":"The Increased aspartate levels in transgenic cotton (Gossypium hirsutum L.) lead to improved tolerance against whitefly (Bemisia tabaci, Gennadius).","authors":"Ambreen Gul, Abdul Qayyum Rao, Mukhtar Ahmed, Ayesha Latif, Allah Bakhsh, Sehrish Iftikhar","doi":"10.1111/ppl.14491","DOIUrl":"https://doi.org/10.1111/ppl.14491","url":null,"abstract":"<p><p>The whitefly, a polyphagous insect pest feeding on nearly 1328 plant species, is a major threat to global cotton production and incurs up to 50% yield losses in cotton production in Pakistan. We investigated whether increased aspartate in phloem sap imparts whitefly toxicity and protects cotton plants from intense damage. The enzymatic step for aspartate production is carried through aspartate aminotransferase (AAT). In this study, we constitutively overexpressed the Oryza sativa cytoplasmic AAT (OsAAT2) under the CaMV35S promoter in Gossypium hirsutum cv. CIM-482. Real-time PCR analysis of the AAT transcripts revealed a 2.85- to 31.7-fold increase in mRNA levels between the different cotton lines. A substantial increase in the free-amino acid content of the major N-assimilation and transport amino acids (aspartate, glutamate, asparagine, and glutamine) was seen in the phloem sap of the transgenic cotton lines. The bioassay revealed that the two transgenic cotton lines with the highest free aspartate content in the phloem sap exhibited 97 and 94% mortality in the adult whitefly population and a 98 and 96% decline in subsequent nymph populations, respectively. There was also a significant change in the physiological behaviour of the transgenic cotton lines, with an increased net assimilation (A), gaseous exchange (Gs) and rate of transpiration (E). Improved morphological characteristics like plant height, total number of bolls and fiber yield were recorded in transgenic cotton lines. The AAT gene shows promise in mitigating whitefly infestations and enhancing the overall health and yield of cotton plants.</p>","PeriodicalId":20164,"journal":{"name":"Physiologia plantarum","volume":null,"pages":null},"PeriodicalIF":5.4,"publicationDate":"2024-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142018337","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Katarzyna Marciniak, Krzysztof Przedniczek, Jacek Kęsy, Wojciech Święcicki, Jan Kopcewicz
The main purpose of this study was to demonstrate that the course of anther development, including post-meiotic maturation, dehiscence and senescence, is ensured by the interdependencies between jasmonic acid (JA) and indole-3-acetic acid (IAA) in yellow lupin (Lupinus luteus L.). The concentration of JA peaked during anther dehiscence when IAA level was low, whereas the inverse relationship was specific to anther senescence. Cellular and tissue localization of JA and IAA, in conjunction with broad expression profile for genes involved in biosynthesis, signalling, response, and homeostasis under different conditions, allowed to complete and define the role of studied phytohormones during late anther development, as well as predict events triggered by them. The development/degeneration of septum and anther wall cells, dehydration of epidermis, and rupture of stomium may involve JA signalling, while the formation of secondary thickening in endothecial cell walls is rather JA independent. The IAA is involved in programmed cell death (PCD)-associated processes during anther senescence but does not exclude its participation in the anther dehiscence processes, mainly related to cell disintegration and degeneration. A detailed understanding of these multistage processes, especially at the level of phytohormonal interplay, can contribute to the effective control of male fertility, potentially revolutionizing the breeding of L. luteus.
本研究的主要目的是证明黄羽扇豆(Lupinus luteus L.)的花药发育过程,包括减数分裂后的成熟、开裂和衰老,是由茉莉酸(JA)和吲哚-3-乙酸(IAA)之间的相互依存关系保证的。当IAA水平较低时,JA的浓度在花药开裂期达到峰值,而在花药衰老期则呈反比关系。JA 和 IAA 的细胞和组织定位,以及在不同条件下参与生物合成、信号传导、反应和平衡的基因的广泛表达谱,有助于完成和确定所研究的植物激素在花药后期发育过程中的作用,以及预测由它们引发的事件。隔膜和花药壁细胞的发育/退化、表皮脱水和气孔破裂可能涉及 JA 信号,而内皮细胞壁次生增厚的形成则与 JA 无关。在花药衰老过程中,IAA 参与了与程序性细胞死亡(PCD)相关的过程,但也不排除它参与了花药开裂过程,主要与细胞解体和变性有关。详细了解这些多阶段过程,尤其是植物激素相互作用的水平,有助于有效控制雄性繁殖力,从而可能彻底改变黄体育种。
{"title":"The development of yellow lupin anthers depends on the relationship between jasmonic acid and indole-3-acetic acid.","authors":"Katarzyna Marciniak, Krzysztof Przedniczek, Jacek Kęsy, Wojciech Święcicki, Jan Kopcewicz","doi":"10.1111/ppl.14385","DOIUrl":"https://doi.org/10.1111/ppl.14385","url":null,"abstract":"<p><p>The main purpose of this study was to demonstrate that the course of anther development, including post-meiotic maturation, dehiscence and senescence, is ensured by the interdependencies between jasmonic acid (JA) and indole-3-acetic acid (IAA) in yellow lupin (Lupinus luteus L.). The concentration of JA peaked during anther dehiscence when IAA level was low, whereas the inverse relationship was specific to anther senescence. Cellular and tissue localization of JA and IAA, in conjunction with broad expression profile for genes involved in biosynthesis, signalling, response, and homeostasis under different conditions, allowed to complete and define the role of studied phytohormones during late anther development, as well as predict events triggered by them. The development/degeneration of septum and anther wall cells, dehydration of epidermis, and rupture of stomium may involve JA signalling, while the formation of secondary thickening in endothecial cell walls is rather JA independent. The IAA is involved in programmed cell death (PCD)-associated processes during anther senescence but does not exclude its participation in the anther dehiscence processes, mainly related to cell disintegration and degeneration. A detailed understanding of these multistage processes, especially at the level of phytohormonal interplay, can contribute to the effective control of male fertility, potentially revolutionizing the breeding of L. luteus.</p>","PeriodicalId":20164,"journal":{"name":"Physiologia plantarum","volume":null,"pages":null},"PeriodicalIF":5.4,"publicationDate":"2024-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141493079","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Tarek Slatni, Imen Ben Slimene, Zina Harzalli, Wael Taamalli, Abderrazak Smaoui, Chedly Abdelly, Salem Elkahoui
The use of plant growth-promoting rhizobacteria (PGPR) in agriculture is one of the most promising approaches to improve plants' growth under salt stress and to support sustainable agriculture under climate change. In this context, our goal was to grow and enhance quinoa growth using native rhizobacteria that can withstand salt stress. To achieve this objective, we isolated rhizobacteria from three saline localities in a semi-arid region in Tunisia, which are characterized by different halophyte species and tested their plant growth-promoting (PGP) activities. Then, we inoculated quinoa seedlings cultivated on 300 mM NaCl with the three most efficient rhizobacteria. A positive effect of the three-salt tolerant rhizobacteria on the growth of quinoa under salinity was observed. In fact, the results of principal component analysis indicated that the inoculation of quinoa by salt-tolerant PGPR under high salinity had a prominent beneficial effect on various growth and physiological parameters of stressed plant, such as the biomass production, the roots length, the secondary roots number, proline content and photosynthesis activities. Three rhizobacteria were utilized in this investigation, and the molecular identification revealed that strain 1 is related to the Bacillus inaquosorum species, strain 2 to Bacillus thuringiensis species and strain 3 to Bacillus proteolyticus species. We can conclude that the saline soil, especially the halophytic rhizosphere, is a potential source of salt-tolerant plant growth-promoting rhizobacteria (ST-PGPR), which stimulate the growth of quinoa and improve its tolerance to salinity.
{"title":"Enhancing quinoa (Chenopodium quinoa) growth in saline environments through salt-tolerant rhizobacteria from halophyte biotope.","authors":"Tarek Slatni, Imen Ben Slimene, Zina Harzalli, Wael Taamalli, Abderrazak Smaoui, Chedly Abdelly, Salem Elkahoui","doi":"10.1111/ppl.14466","DOIUrl":"https://doi.org/10.1111/ppl.14466","url":null,"abstract":"<p><p>The use of plant growth-promoting rhizobacteria (PGPR) in agriculture is one of the most promising approaches to improve plants' growth under salt stress and to support sustainable agriculture under climate change. In this context, our goal was to grow and enhance quinoa growth using native rhizobacteria that can withstand salt stress. To achieve this objective, we isolated rhizobacteria from three saline localities in a semi-arid region in Tunisia, which are characterized by different halophyte species and tested their plant growth-promoting (PGP) activities. Then, we inoculated quinoa seedlings cultivated on 300 mM NaCl with the three most efficient rhizobacteria. A positive effect of the three-salt tolerant rhizobacteria on the growth of quinoa under salinity was observed. In fact, the results of principal component analysis indicated that the inoculation of quinoa by salt-tolerant PGPR under high salinity had a prominent beneficial effect on various growth and physiological parameters of stressed plant, such as the biomass production, the roots length, the secondary roots number, proline content and photosynthesis activities. Three rhizobacteria were utilized in this investigation, and the molecular identification revealed that strain 1 is related to the Bacillus inaquosorum species, strain 2 to Bacillus thuringiensis species and strain 3 to Bacillus proteolyticus species. We can conclude that the saline soil, especially the halophytic rhizosphere, is a potential source of salt-tolerant plant growth-promoting rhizobacteria (ST-PGPR), which stimulate the growth of quinoa and improve its tolerance to salinity.</p>","PeriodicalId":20164,"journal":{"name":"Physiologia plantarum","volume":null,"pages":null},"PeriodicalIF":5.4,"publicationDate":"2024-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142009218","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Xanthomonas oryzae pv. oryzae (Xoo), the causative agent of bacterial blight (BB), has developed a unique strategy to infect rice by hijacking the host's methylglyoxal (MG) detoxification pathway. This results in an over-accumulation of MG, which facilitates tissue colonization and evasion of host's immune responses. While MG role in abiotic stresses is well-documented, its involvement in biotic stresses has not been extensively explored. Recently, Fu et al. (2024) provided the first evidence of MG role in promoting Xoo pathogenesis in rice. This new virulence strategy contributes to the pathogen's remarkable adaptability and survival. In this mechanism of hijacking of MG detoxification pathway, Xoo induces OsWRKY62.1 to inhibit OsGLY II expression, leading to MG overaccumulation in infected rice cells. This excess MG hinders plant cell organelle function, creating a favorable environment for Xoo by compromising the rice defense system. In this article, we have presented our perspectives on how the BB pathogen adapts its virulence mechanisms to infect and cause disease in rice.
{"title":"Hijacking of the methylglyoxal detoxification pathway: a new tactic of Xoo pathogenesis in rice.","authors":"Parvesh Kumar, Poonam Kumari, Rakesh Mehra, Bahaderjeet Singh, Rakesh Kumar","doi":"10.1111/ppl.14439","DOIUrl":"https://doi.org/10.1111/ppl.14439","url":null,"abstract":"<p><p>Xanthomonas oryzae pv. oryzae (Xoo), the causative agent of bacterial blight (BB), has developed a unique strategy to infect rice by hijacking the host's methylglyoxal (MG) detoxification pathway. This results in an over-accumulation of MG, which facilitates tissue colonization and evasion of host's immune responses. While MG role in abiotic stresses is well-documented, its involvement in biotic stresses has not been extensively explored. Recently, Fu et al. (2024) provided the first evidence of MG role in promoting Xoo pathogenesis in rice. This new virulence strategy contributes to the pathogen's remarkable adaptability and survival. In this mechanism of hijacking of MG detoxification pathway, Xoo induces OsWRKY62.1 to inhibit OsGLY II expression, leading to MG overaccumulation in infected rice cells. This excess MG hinders plant cell organelle function, creating a favorable environment for Xoo by compromising the rice defense system. In this article, we have presented our perspectives on how the BB pathogen adapts its virulence mechanisms to infect and cause disease in rice.</p>","PeriodicalId":20164,"journal":{"name":"Physiologia plantarum","volume":null,"pages":null},"PeriodicalIF":5.4,"publicationDate":"2024-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141590954","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Cadmium (Cd) is a leading environmental issue worldwide. The current study was conducted to investigate Cd tolerance of 10 commercial white clover (Trifolium repens) cultivars during seed germination and to further explore differences in lipid remodelling, glycometabolism, and the conversion of lipids into sugars contributing to Cd tolerance in the early phase of seedling establishment as well as the accumulation of Cd in seedlings and mature plants. The results show that Cd stress significantly reduced seed germination of 10 cultivars. Compared to Cd-sensitive Sulky, Cd-tolerant Pixie accelerated amylolysis to produce more glucose, fructose, and sucrose by maintaining higher amylase and sucrase activities under Cd stress. Pixie maintained higher contents of various lipids, higher DGDG/MGDG ratio, and lower unsaturation levels of lipids, which could be beneficial to membrane stability and integrity as well as signal transduction in cells after being subjected to Cd stress. In addition, Pixie upregulated expression levels of key genes (TrACX1, TrACX4, TrSDP6, and TrPCK1) involved in the conversion of lipids into sugars for early seedling establishment under Cd stress. These findings indicate that lipid remodelling, enhanced glycometabolism, and accelerated conversion of lipids into sugars are important adaptive strategies for white clover seed germination and subsequent seedling establishment under Cd stress. In addition, Pixie not only accumulated more Cd in seedlings and mature plants than Sulky but also had significantly better growth and phytoremediation efficiency under Cd stress. Pixie could be used as a suitable and critical germplasm for the rehabilitation and re-establishment of Cd-contaminated areas.
{"title":"Lipid remodelling and the conversion of lipids into sugars associated with tolerance to cadmium toxicity during white clover seed germination.","authors":"Long Lin, Junnan Lin, Min Zhou, Yan Yuan, Zhou Li","doi":"10.1111/ppl.14433","DOIUrl":"https://doi.org/10.1111/ppl.14433","url":null,"abstract":"<p><p>Cadmium (Cd) is a leading environmental issue worldwide. The current study was conducted to investigate Cd tolerance of 10 commercial white clover (Trifolium repens) cultivars during seed germination and to further explore differences in lipid remodelling, glycometabolism, and the conversion of lipids into sugars contributing to Cd tolerance in the early phase of seedling establishment as well as the accumulation of Cd in seedlings and mature plants. The results show that Cd stress significantly reduced seed germination of 10 cultivars. Compared to Cd-sensitive Sulky, Cd-tolerant Pixie accelerated amylolysis to produce more glucose, fructose, and sucrose by maintaining higher amylase and sucrase activities under Cd stress. Pixie maintained higher contents of various lipids, higher DGDG/MGDG ratio, and lower unsaturation levels of lipids, which could be beneficial to membrane stability and integrity as well as signal transduction in cells after being subjected to Cd stress. In addition, Pixie upregulated expression levels of key genes (TrACX1, TrACX4, TrSDP6, and TrPCK1) involved in the conversion of lipids into sugars for early seedling establishment under Cd stress. These findings indicate that lipid remodelling, enhanced glycometabolism, and accelerated conversion of lipids into sugars are important adaptive strategies for white clover seed germination and subsequent seedling establishment under Cd stress. In addition, Pixie not only accumulated more Cd in seedlings and mature plants than Sulky but also had significantly better growth and phytoremediation efficiency under Cd stress. Pixie could be used as a suitable and critical germplasm for the rehabilitation and re-establishment of Cd-contaminated areas.</p>","PeriodicalId":20164,"journal":{"name":"Physiologia plantarum","volume":null,"pages":null},"PeriodicalIF":5.4,"publicationDate":"2024-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141590955","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
There is a focus on sustainability when manufacturing materials. Utilizing biobased materials and replacing fossil-based products is the main research focus. Bio-composite materials are applied to packaging, filler coatings, and pharmaceuticals. Here, we used the leaves of the agro-waste plant Albizia lebeck L. to extract cellulose. Chemical treatment causing strong acid hydrolysis successfully extracted the cellulose content from the leaves. The cellulose obtained was then strengthened with polylactic acid to make a biobased film for future applications. Fourier transform spectroscopy, scanning electron microscopy, thermal analysis, particle size analysis, visible UV and elemental analysis were all used to characterize the extracted cellulose. SEM and mechanical property analysis were used to check and describe the quality of the reinforced biofilm. The greatest cellulose yield from this raw material was 50.2%. The crystallinity index and crystallite size (CI 70.3% and CS 11.29 nm) were high in the extracted cellulose. The TG (DTG) curve analysis derivative revealed cellulose particle breakdown was initiated around 305.2°C and can endure temperatures up to 600°C. Biofilms reinforced with polylactic acid cellulose (1, 2, 3, and 5% by weight %) exhibited a smooth and parallel surface. As the filler concentration increased, minor agglomeration occurred. The tensile strength of pure polylactic acid (PLA) (34.72 MPa) was extended up to 38.91 MPa for 5% filler. Similarly, Young's modulus also increased to 5.24 MPa. However, the elongation break decreases with the increase of filler content, and the least value of decrease is 7.5 MPa. Concerning prospective implementations, it is expected that the biobased film and cellulose particles will prove to be more functional.
{"title":"Sustainable microcrystalline cellulose extracted from biowaste Albezia lebeck L. leaves: Biomass exfoliation and physicochemical characterization.","authors":"Murugesan Palaniappan","doi":"10.1111/ppl.14447","DOIUrl":"https://doi.org/10.1111/ppl.14447","url":null,"abstract":"<p><p>There is a focus on sustainability when manufacturing materials. Utilizing biobased materials and replacing fossil-based products is the main research focus. Bio-composite materials are applied to packaging, filler coatings, and pharmaceuticals. Here, we used the leaves of the agro-waste plant Albizia lebeck L. to extract cellulose. Chemical treatment causing strong acid hydrolysis successfully extracted the cellulose content from the leaves. The cellulose obtained was then strengthened with polylactic acid to make a biobased film for future applications. Fourier transform spectroscopy, scanning electron microscopy, thermal analysis, particle size analysis, visible UV and elemental analysis were all used to characterize the extracted cellulose. SEM and mechanical property analysis were used to check and describe the quality of the reinforced biofilm. The greatest cellulose yield from this raw material was 50.2%. The crystallinity index and crystallite size (CI 70.3% and CS 11.29 nm) were high in the extracted cellulose. The TG (DTG) curve analysis derivative revealed cellulose particle breakdown was initiated around 305.2°C and can endure temperatures up to 600°C. Biofilms reinforced with polylactic acid cellulose (1, 2, 3, and 5% by weight %) exhibited a smooth and parallel surface. As the filler concentration increased, minor agglomeration occurred. The tensile strength of pure polylactic acid (PLA) (34.72 MPa) was extended up to 38.91 MPa for 5% filler. Similarly, Young's modulus also increased to 5.24 MPa. However, the elongation break decreases with the increase of filler content, and the least value of decrease is 7.5 MPa. Concerning prospective implementations, it is expected that the biobased film and cellulose particles will prove to be more functional.</p>","PeriodicalId":20164,"journal":{"name":"Physiologia plantarum","volume":null,"pages":null},"PeriodicalIF":5.4,"publicationDate":"2024-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141988537","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Samuel Minguillón, Katrin Fischer-Schrader, Carmen Pérez-Rontomé, Manuel A Matamoros, Manuel Becana
Human mitochondria contain a molybdoprotein capable of reducing amidoximes using cytochrome b5/cytochrome b5 reductase (Cb/CbR). This 'amidoxime reducing component' (ARC) also reduces nitrite to nitric oxide (NO). In the plant kingdom, distinct functions have been suggested for ARCs. Thus, the single ARC of Chlamydomonas reinhardtii (crARC) reduces nitrite to NO by taking electrons from nitrate reductase (NR). Therefore, it was proposed that a dual NR/crARC system can generate NO under physiological conditions and the crARC was renamed to 'NO-forming nitrite reductase' (NOFNiR). In contrast to this, the two ARC enzymes from Arabidopsis thaliana were not found to produce NO in vitro at physiological nitrite concentrations, suggesting a different, as yet unknown, function in vascular plants. Here, we have investigated the two ARCs of Lotus japonicus (LjARCs) to shed light on this controversy and to examine, for the first time, the distribution of ARCs in plant tissues. The LjARCs are localized in the cytosol and their activities and catalytic efficiencies, which are much higher than those of A. thaliana, are consistent with a role as NOFNiR. LjARCs are prone to S-nitrosylation in vitro by S-nitrosoglutathione and this post-translational modification drastically inhibits their activities. The enzymes are mainly expressed in flowers, seeds and pods, but are absent in nodules. LjARCs are active with NR and Cb/CbR as electron-transferring systems. However, the LjNR mRNA levels in seeds and pods are negligible, whereas our proteomic analyses show that pods contain the two ARCs, Cb and CbR. We conclude that LjARCs may play a role as NOFNiR by receiving electrons from the Cb/CbR system but do not act in combination with NR.
{"title":"The enigmatic enzyme 'amidoxime reducing component' of Lotus japonicus. Characterization, expression, activity in plant tissues, and proposed role as a nitric oxide-forming nitrite reductase.","authors":"Samuel Minguillón, Katrin Fischer-Schrader, Carmen Pérez-Rontomé, Manuel A Matamoros, Manuel Becana","doi":"10.1111/ppl.14438","DOIUrl":"https://doi.org/10.1111/ppl.14438","url":null,"abstract":"<p><p>Human mitochondria contain a molybdoprotein capable of reducing amidoximes using cytochrome b<sub>5</sub>/cytochrome b<sub>5</sub> reductase (Cb/CbR). This 'amidoxime reducing component' (ARC) also reduces nitrite to nitric oxide (NO). In the plant kingdom, distinct functions have been suggested for ARCs. Thus, the single ARC of Chlamydomonas reinhardtii (crARC) reduces nitrite to NO by taking electrons from nitrate reductase (NR). Therefore, it was proposed that a dual NR/crARC system can generate NO under physiological conditions and the crARC was renamed to 'NO-forming nitrite reductase' (NOFNiR). In contrast to this, the two ARC enzymes from Arabidopsis thaliana were not found to produce NO in vitro at physiological nitrite concentrations, suggesting a different, as yet unknown, function in vascular plants. Here, we have investigated the two ARCs of Lotus japonicus (LjARCs) to shed light on this controversy and to examine, for the first time, the distribution of ARCs in plant tissues. The LjARCs are localized in the cytosol and their activities and catalytic efficiencies, which are much higher than those of A. thaliana, are consistent with a role as NOFNiR. LjARCs are prone to S-nitrosylation in vitro by S-nitrosoglutathione and this post-translational modification drastically inhibits their activities. The enzymes are mainly expressed in flowers, seeds and pods, but are absent in nodules. LjARCs are active with NR and Cb/CbR as electron-transferring systems. However, the LjNR mRNA levels in seeds and pods are negligible, whereas our proteomic analyses show that pods contain the two ARCs, Cb and CbR. We conclude that LjARCs may play a role as NOFNiR by receiving electrons from the Cb/CbR system but do not act in combination with NR.</p>","PeriodicalId":20164,"journal":{"name":"Physiologia plantarum","volume":null,"pages":null},"PeriodicalIF":5.4,"publicationDate":"2024-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141760346","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Late embryogenesis abundant (LEA) proteins have been widely recognized for their role in various abiotic stress responses in higher plants. Nevertheless, the specific mechanism responsible for the function of LEA proteins in plants has not yet been explored. This research involved the isolation and characterization of HcLEA113 from kenaf, revealing a significant increase in its expression in response to drought stress. When HcLEA113 was introduced into yeast, it resulted in an improved survival rate under drought conditions. Furthermore, the overexpression of HcLEA113 in tobacco plants led to enhanced tolerance to drought stress. Specifically, HcLEA113-OE plants exhibited higher germination rates, longer root lengths, greater chlorophyll content, and higher relative water content under drought stress compared to wild-type (WT) plants, while their relative conductivity was significantly lower than that of WT plants. Further physiological measurements revealed that the proline content, soluble sugars, and antioxidant activities of WT and HcLEA113-OE tobacco leaves increased significantly under drought stress, with greater changes in HcLEA113-OE plants than WT. The increase in hydrogen peroxide (H2O2), superoxide anions (O2-), and malondialdehyde (MDA) content was significantly lower in HcLEA113-OE lines than in WT plants. Additionally, HcLEA113-OE plants can activate reactive oxygen species (ROS)- and osmotic-related genes in response to drought stress. On the other hand, silencing the HcLEA113 gene through virus-induced gene silencing (VIGS) in kenaf plants led to notable growth suppression when exposed to drought conditions, manifesting as decreased plant height and dry weight. Meanwhile, antioxidant enzymes' activity significantly decreased and the ROS content increased. This study offers valuable insights for future research on the genetic engineering of drought resistance in plants.
{"title":"HcLEA113, a late embryogenesis abundant protein gene, positively regulates drought-stress responses in kenaf.","authors":"Dengjie Luo, Caijin Wang, Samavia Mubeen, Muzammal Rehman, Shan Cao, Jiao Yue, Jiao Pan, Gang Jin, Ru Li, Tao Chen, Peng Chen","doi":"10.1111/ppl.14506","DOIUrl":"10.1111/ppl.14506","url":null,"abstract":"<p><p>Late embryogenesis abundant (LEA) proteins have been widely recognized for their role in various abiotic stress responses in higher plants. Nevertheless, the specific mechanism responsible for the function of LEA proteins in plants has not yet been explored. This research involved the isolation and characterization of HcLEA113 from kenaf, revealing a significant increase in its expression in response to drought stress. When HcLEA113 was introduced into yeast, it resulted in an improved survival rate under drought conditions. Furthermore, the overexpression of HcLEA113 in tobacco plants led to enhanced tolerance to drought stress. Specifically, HcLEA113-OE plants exhibited higher germination rates, longer root lengths, greater chlorophyll content, and higher relative water content under drought stress compared to wild-type (WT) plants, while their relative conductivity was significantly lower than that of WT plants. Further physiological measurements revealed that the proline content, soluble sugars, and antioxidant activities of WT and HcLEA113-OE tobacco leaves increased significantly under drought stress, with greater changes in HcLEA113-OE plants than WT. The increase in hydrogen peroxide (H<sub>2</sub>O<sub>2</sub>), superoxide anions (O<sub>2</sub> <sup>-</sup>), and malondialdehyde (MDA) content was significantly lower in HcLEA113-OE lines than in WT plants. Additionally, HcLEA113-OE plants can activate reactive oxygen species (ROS)- and osmotic-related genes in response to drought stress. On the other hand, silencing the HcLEA113 gene through virus-induced gene silencing (VIGS) in kenaf plants led to notable growth suppression when exposed to drought conditions, manifesting as decreased plant height and dry weight. Meanwhile, antioxidant enzymes' activity significantly decreased and the ROS content increased. This study offers valuable insights for future research on the genetic engineering of drought resistance in plants.</p>","PeriodicalId":20164,"journal":{"name":"Physiologia plantarum","volume":null,"pages":null},"PeriodicalIF":5.4,"publicationDate":"2024-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142081290","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}