首页 > 最新文献

Physiologia plantarum最新文献

英文 中文
GhJUB1_3-At positively regulate drought and salt stress tolerance under control of GhHB7, GhRAP2-3 and GhRAV1 in Cotton. 在 GhHB7、GhRAP2-3 和 GhRAV1 的调控下,GhJUB1_3-At 可正向调节棉花的干旱和盐胁迫耐受性。
IF 5.4 2区 生物学 Q1 PLANT SCIENCES Pub Date : 2024-09-01 DOI: 10.1111/ppl.14497
Adeel Ahmad, Muhammad Sajjad, Salisu Bello Sadau, Mohammad Elasad, Lu Sun, Yuewei Quan, Aimin Wu, Lian Boying, Fei Wei, Hongmei Wu, Pengyun Chen, Xiaokang Fu, Liang Ma, Hantao Wang, Hengling Wei, Shuxun Yu

Climate change severely affects crop production. Cotton is one of the primary fiber crops in the world and its production is susceptible to various environmental stresses, especially drought and salinity. Development of stress tolerant genotypes is the only way to escape from these environmental constraints. We identified sixteen homologs of the Arabidopsis JUB1 gene in cotton. Expression of GhJUB1_3-At was significantly induced in the temporal expression analysis of GhJUB1 genes in the roots of drought tolerant (H177) and susceptible (S9612) cotton genotypes under drought. The silencing of the GhJUB1_3-At gene alone and together with its paralogue GhJUB1_3-Dt reduced the drought tolerance in cotton plants. The transgenic lines exhibited tolerance to the drought and salt stress as compared to the wildtype (WT). The chlorophyll and relative water contents of wildtype decreased under drought as compared to the transgenic lines. The transgenic lines showed decreased H2O2 and increased proline levels under drought and salt stress, as compared to the WT, indicating that the transgenic lines have drought and salt stress tolerance. The expression analysis of the transgenic lines and WT revealed that GAI was upregulated in the transgenic lines in normal conditions as compared to the WT. Under drought and salt treatment, RAB18 and RD29A were strongly upregulated in the transgenic lines as compared to the WT. Conclusively, GhJUB1_3-At is not an auto activator and it is regulated by the crosstalk of GhHB7, GhRAP2-3 and GhRAV1. GhRAV1, a negative regulator of abiotic stress tolerance and positive regulator of leaf senescence, suppresses the expression of GhJUB1_3-At under severe circumstances leading to plant death.

气候变化严重影响作物生产。棉花是世界上最主要的纤维作物之一,其生产容易受到各种环境压力的影响,尤其是干旱和盐渍化。开发抗逆基因型是摆脱这些环境限制的唯一途径。我们在棉花中发现了 16 个拟南芥 JUB1 基因的同源物。在对耐旱(H177)和易感(S9612)棉花基因型根中 GhJUB1 基因的时序表达分析中,GhJUB1_3-At 的表达被显著诱导。GhJUB1_3-At 基因单独或与其旁系亲属 GhJUB1_3-Dt 基因一起沉默都会降低棉花植株的耐旱性。与野生型(WT)相比,转基因品系表现出对干旱和盐胁迫的耐受性。与转基因品系相比,野生型在干旱条件下叶绿素和相对含水量都有所下降。与 WT 相比,转基因品系在干旱和盐胁迫下的 H2O2 水平降低,脯氨酸水平升高,这表明转基因品系具有抗旱和抗盐胁迫的能力。对转基因株系和 WT 的表达分析表明,与 WT 相比,转基因株系在正常条件下 GAI 上调。在干旱和盐胁迫条件下,与 WT 相比,RAB18 和 RD29A 在转基因品系中强烈上调。结论是,GhJUB1_3-At 并不是一个自动激活因子,它是由 GhHB7、GhRAP2-3 和 GhRAV1 共同调控的。GhRAV1 是非生物胁迫耐受性的负调控因子和叶片衰老的正调控因子,在严重情况下会抑制 GhJUB1_3-At 的表达,导致植物死亡。
{"title":"GhJUB1_3-At positively regulate drought and salt stress tolerance under control of GhHB7, GhRAP2-3 and GhRAV1 in Cotton.","authors":"Adeel Ahmad, Muhammad Sajjad, Salisu Bello Sadau, Mohammad Elasad, Lu Sun, Yuewei Quan, Aimin Wu, Lian Boying, Fei Wei, Hongmei Wu, Pengyun Chen, Xiaokang Fu, Liang Ma, Hantao Wang, Hengling Wei, Shuxun Yu","doi":"10.1111/ppl.14497","DOIUrl":"10.1111/ppl.14497","url":null,"abstract":"<p><p>Climate change severely affects crop production. Cotton is one of the primary fiber crops in the world and its production is susceptible to various environmental stresses, especially drought and salinity. Development of stress tolerant genotypes is the only way to escape from these environmental constraints. We identified sixteen homologs of the Arabidopsis JUB1 gene in cotton. Expression of GhJUB1_3-At was significantly induced in the temporal expression analysis of GhJUB1 genes in the roots of drought tolerant (H177) and susceptible (S9612) cotton genotypes under drought. The silencing of the GhJUB1_3-At gene alone and together with its paralogue GhJUB1_3-Dt reduced the drought tolerance in cotton plants. The transgenic lines exhibited tolerance to the drought and salt stress as compared to the wildtype (WT). The chlorophyll and relative water contents of wildtype decreased under drought as compared to the transgenic lines. The transgenic lines showed decreased H<sub>2</sub>O<sub>2</sub> and increased proline levels under drought and salt stress, as compared to the WT, indicating that the transgenic lines have drought and salt stress tolerance. The expression analysis of the transgenic lines and WT revealed that GAI was upregulated in the transgenic lines in normal conditions as compared to the WT. Under drought and salt treatment, RAB18 and RD29A were strongly upregulated in the transgenic lines as compared to the WT. Conclusively, GhJUB1_3-At is not an auto activator and it is regulated by the crosstalk of GhHB7, GhRAP2-3 and GhRAV1. GhRAV1, a negative regulator of abiotic stress tolerance and positive regulator of leaf senescence, suppresses the expression of GhJUB1_3-At under severe circumstances leading to plant death.</p>","PeriodicalId":20164,"journal":{"name":"Physiologia plantarum","volume":"176 5","pages":"e14497"},"PeriodicalIF":5.4,"publicationDate":"2024-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142120374","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Plant Growth Promoting Rhizobacteria (PGPR) induced protection: A plant immunity perspective. 植物生长促进根瘤菌(PGPR)诱导保护:植物免疫的视角。
IF 5.4 2区 生物学 Q1 PLANT SCIENCES Pub Date : 2024-09-01 DOI: 10.1111/ppl.14495
Rinkee Kumari, Ekta Pandey, Sayyada Bushra, Shahla Faizan, Saurabh Pandey

Plant-environment interactions, particularly biotic stress, are increasingly essential for global food security due to crop losses in the dynamic environment. Therefore, understanding plant responses to biotic stress is vital to mitigate damage. Beneficial microorganisms and their association with plants can reduce the damage associated with plant pathogens. One such group is PGPR (Plant growth-promoting rhizobacteria), which influences plant immunity significantly by interacting with biotic stress factors and plant signalling compounds. This review explores the types, metabolism, and mechanisms of action of PGPR, including their enzyme pathways and the signalling compounds secreted by PGPR that modulate gene and protein expression during plant defence. Furthermore, the review will delve into the crosstalk between PGPR and other plant growth regulators and signalling compounds, elucidating the physiological, biochemical, and molecular insights into PGPR's impact on plants under multiple biotic stresses, including interactions with fungi, bacteria, and viruses. Overall, the review comprehensively adds to our knowledge about PGPR's role in plant immunity and its application for agricultural resilience and food security.

植物与环境之间的相互作用,特别是生物胁迫,对全球粮食安全越来越重要,因为在动态环境中会造成作物损失。因此,了解植物对生物胁迫的反应对于减轻损害至关重要。有益微生物及其与植物的联系可以减轻植物病原体造成的损害。PGPR(植物生长促进根瘤菌)就是其中之一,它通过与生物胁迫因子和植物信号化合物相互作用,对植物免疫力产生重大影响。本综述探讨了 PGPR 的类型、新陈代谢和作用机制,包括它们的酶途径和 PGPR 分泌的信号化合物,这些化合物可在植物防御过程中调节基因和蛋白质的表达。此外,综述还将深入探讨 PGPR 与其他植物生长调节剂和信号化合物之间的相互影响,阐明 PGPR 在多种生物胁迫下对植物产生影响的生理、生化和分子机制,包括与真菌、细菌和病毒之间的相互作用。总之,这篇综述全面补充了我们关于 PGPR 在植物免疫中的作用及其在农业抗逆性和粮食安全方面的应用的知识。
{"title":"Plant Growth Promoting Rhizobacteria (PGPR) induced protection: A plant immunity perspective.","authors":"Rinkee Kumari, Ekta Pandey, Sayyada Bushra, Shahla Faizan, Saurabh Pandey","doi":"10.1111/ppl.14495","DOIUrl":"10.1111/ppl.14495","url":null,"abstract":"<p><p>Plant-environment interactions, particularly biotic stress, are increasingly essential for global food security due to crop losses in the dynamic environment. Therefore, understanding plant responses to biotic stress is vital to mitigate damage. Beneficial microorganisms and their association with plants can reduce the damage associated with plant pathogens. One such group is PGPR (Plant growth-promoting rhizobacteria), which influences plant immunity significantly by interacting with biotic stress factors and plant signalling compounds. This review explores the types, metabolism, and mechanisms of action of PGPR, including their enzyme pathways and the signalling compounds secreted by PGPR that modulate gene and protein expression during plant defence. Furthermore, the review will delve into the crosstalk between PGPR and other plant growth regulators and signalling compounds, elucidating the physiological, biochemical, and molecular insights into PGPR's impact on plants under multiple biotic stresses, including interactions with fungi, bacteria, and viruses. Overall, the review comprehensively adds to our knowledge about PGPR's role in plant immunity and its application for agricultural resilience and food security.</p>","PeriodicalId":20164,"journal":{"name":"Physiologia plantarum","volume":"176 5","pages":"e14495"},"PeriodicalIF":5.4,"publicationDate":"2024-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142154786","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
A resource of identified and annotated lincRNAs expressed during somatic embryogenesis development in Norway spruce. 挪威云杉体细胞胚胎发育过程中表达的已识别和注释的 lincRNA 资源。
IF 5.4 2区 生物学 Q1 PLANT SCIENCES Pub Date : 2024-09-01 DOI: 10.1111/ppl.14537
Camilla Canovi, Katja Stojkovič, Aarón Ayllón Benítez, Nicolas Delhomme, Ulrika Egertsdotter, Nathaniel R Street

Long non-coding RNAs (lncRNAs) have emerged as important regulators of many biological processes, although their regulatory roles remain poorly characterized in woody plants, especially in gymnosperms. A major challenge of working with lncRNAs is to assign functional annotations, since they have a low coding potential and low cross-species conservation. We utilised an existing RNA-Sequencing resource and performed short RNA sequencing of somatic embryogenesis developmental stages in Norway spruce (Picea abies L. Karst). We implemented a pipeline to identify lncRNAs located within the intergenic space (lincRNAs) and generated a co-expression network including protein coding, lincRNA and miRNA genes. To assign putative functional annotation, we employed a guilt-by-association approach using the co-expression network and integrated these results with annotation assigned using semantic similarity and co-expression. Moreover, we evaluated the relationship between lincRNAs and miRNAs, and identified which lincRNAs are conserved in other species. We identified lincRNAs with clear evidence of differential expression during somatic embryogenesis and used network connectivity to identify those with the greatest regulatory potential. This work provides the most comprehensive view of lincRNAs in Norway spruce and is the first study to perform global identification of lincRNAs during somatic embryogenesis in conifers. The data have been integrated into the expression visualisation tools at the PlantGenIE.org web resource to enable easy access to the community. This will facilitate the use of the data to address novel questions about the role of lincRNAs in the regulation of embryogenesis and facilitate future comparative genomics studies.

长非编码 RNA(lncRNA)已成为许多生物过程的重要调控因子,但它们在木本植物(尤其是裸子植物)中的调控作用仍不十分明确。由于 lncRNA 的编码潜力低且跨物种保护程度低,因此对其进行功能注释是一项重大挑战。我们利用现有的 RNA 测序资源,对挪威云杉(Picea abies L. Karst)的体细胞胚胎发育阶段进行了短 RNA 测序。我们采用了一个管道来识别位于基因间空间(lincRNAs)的lncRNAs,并生成了一个包括蛋白质编码、lincRNA和miRNA基因的共表达网络。为了分配推定的功能注释,我们利用共表达网络采用了一种 "按关联判罪 "的方法,并将这些结果与利用语义相似性和共表达分配的注释进行了整合。此外,我们还评估了 lincRNA 与 miRNA 之间的关系,并确定了哪些 lincRNA 在其他物种中是保守的。我们确定了在体细胞胚胎发生过程中具有明确差异表达证据的 lincRNA,并利用网络连接性确定了那些具有最大调控潜力的 lincRNA。这项工作提供了对挪威云杉中lincRNA的最全面了解,也是首次对针叶树体细胞胚胎发生过程中的lincRNA进行全球鉴定的研究。这些数据已被整合到PlantGenIE.org网络资源的表达可视化工具中,以方便社区访问。这将有助于利用这些数据解决有关 lincRNA 在胚胎发生调控过程中的作用的新问题,并促进未来的比较基因组学研究。
{"title":"A resource of identified and annotated lincRNAs expressed during somatic embryogenesis development in Norway spruce.","authors":"Camilla Canovi, Katja Stojkovič, Aarón Ayllón Benítez, Nicolas Delhomme, Ulrika Egertsdotter, Nathaniel R Street","doi":"10.1111/ppl.14537","DOIUrl":"https://doi.org/10.1111/ppl.14537","url":null,"abstract":"<p><p>Long non-coding RNAs (lncRNAs) have emerged as important regulators of many biological processes, although their regulatory roles remain poorly characterized in woody plants, especially in gymnosperms. A major challenge of working with lncRNAs is to assign functional annotations, since they have a low coding potential and low cross-species conservation. We utilised an existing RNA-Sequencing resource and performed short RNA sequencing of somatic embryogenesis developmental stages in Norway spruce (Picea abies L. Karst). We implemented a pipeline to identify lncRNAs located within the intergenic space (lincRNAs) and generated a co-expression network including protein coding, lincRNA and miRNA genes. To assign putative functional annotation, we employed a guilt-by-association approach using the co-expression network and integrated these results with annotation assigned using semantic similarity and co-expression. Moreover, we evaluated the relationship between lincRNAs and miRNAs, and identified which lincRNAs are conserved in other species. We identified lincRNAs with clear evidence of differential expression during somatic embryogenesis and used network connectivity to identify those with the greatest regulatory potential. This work provides the most comprehensive view of lincRNAs in Norway spruce and is the first study to perform global identification of lincRNAs during somatic embryogenesis in conifers. The data have been integrated into the expression visualisation tools at the PlantGenIE.org web resource to enable easy access to the community. This will facilitate the use of the data to address novel questions about the role of lincRNAs in the regulation of embryogenesis and facilitate future comparative genomics studies.</p>","PeriodicalId":20164,"journal":{"name":"Physiologia plantarum","volume":"176 5","pages":"e14537"},"PeriodicalIF":5.4,"publicationDate":"2024-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142352047","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Root physiological and soil microbial mechanisms underlying responses to nitrogen deficiency and compensation in Indica and Japonica rice. 籼稻和粳稻对氮素缺乏和补偿反应的根系生理和土壤微生物机制。
IF 5.4 2区 生物学 Q1 PLANT SCIENCES Pub Date : 2024-09-01 DOI: 10.1111/ppl.14549
Runnan Wang, Guoping Tang, Yanyao Lu, Dingshun Zhang, Shuo Cai, Haohua He, Hongcheng Zhang, Qiangqiang Xiong

Compensatory effects are common biological phenomena in nature. In this study, we investigated the changes in root nitrogen uptake, root morphological and physiological responses, and changes in the rhizosphere soil microbial communities of indica and japonica rice during a nitrogen deficiency-sensitive period and an effective compensation period with double the nitrogen supply. We conducted a bucket experiment using Suxiu 867 (a japonica rice variety) and Yangxian You 918 (an indica rice variety). Treatments included CK (constant distribution of nitrogen fertilizer at each growth stage, represented by CK867 and CK918) and NDC (nitrogen deficiency in the tillering stage, double nitrogen application in the ear differentiation stage to compensate, represented by NDC867 and NDC918) variations. Both varieties presented the highest δ15N and 15N abundances and Ndff (refers to the proportion of nitrogen in a plant's body that comes directly from the fertilizer applied.) in rice under the NDC treatment. Metagenomic sequencing of rhizospheric soil showed that the dominant bacterial groups at the phylum level among each treatment were Actinobacteria, Proteobacteria, Chloroflexi, Acidobacteria, Gemmatimonadetes, and Nitrospirae. The rhizosphere of indica rice was more enriched with the microbial communities involved in nitrogen metabolism, which contributed to higher nitrogen utilization efficiency. A correlation-based network was constructed and provides insights into the formation of nitrogen deficiency compensation effects and contributes to the enhancement of nitrogen uptake and utilization efficiency in rice production.

补偿效应是自然界常见的生物现象。本研究考察了籼稻和粳稻在缺氮敏感期和双倍氮素供应的有效补偿期根系氮素吸收、根系形态和生理反应的变化,以及根圈土壤微生物群落的变化。我们使用苏秀 867(粳稻品种)和扬籼优 918(籼稻品种)进行了水桶实验。处理包括 CK(在每个生长阶段恒定分配氮肥,以 CK867 和 CK918 为代表)和 NDC(分蘖期缺氮,在穗分化期加倍施氮进行补偿,以 NDC867 和 NDC918 为代表)变化。这两个品种的水稻在 NDC 处理下的δ15N 和 15N 丰度以及 Ndff(指植物体内直接来自所施肥料的氮的比例)都是最高的。根瘤土壤的元基因组测序结果表明,在各处理中,门级的优势细菌群分别为放线菌、蛋白质细菌、绿僵菌、酸性细菌、革马拉菌和硝化细菌。籼稻根瘤菌群中参与氮代谢的微生物群落更为丰富,从而提高了氮的利用效率。该研究构建了一个基于相关性的网络,有助于深入了解缺氮补偿效应的形成,并有助于提高水稻生产中的氮吸收和利用效率。
{"title":"Root physiological and soil microbial mechanisms underlying responses to nitrogen deficiency and compensation in Indica and Japonica rice.","authors":"Runnan Wang, Guoping Tang, Yanyao Lu, Dingshun Zhang, Shuo Cai, Haohua He, Hongcheng Zhang, Qiangqiang Xiong","doi":"10.1111/ppl.14549","DOIUrl":"https://doi.org/10.1111/ppl.14549","url":null,"abstract":"<p><p>Compensatory effects are common biological phenomena in nature. In this study, we investigated the changes in root nitrogen uptake, root morphological and physiological responses, and changes in the rhizosphere soil microbial communities of indica and japonica rice during a nitrogen deficiency-sensitive period and an effective compensation period with double the nitrogen supply. We conducted a bucket experiment using Suxiu 867 (a japonica rice variety) and Yangxian You 918 (an indica rice variety). Treatments included CK (constant distribution of nitrogen fertilizer at each growth stage, represented by CK867 and CK918) and NDC (nitrogen deficiency in the tillering stage, double nitrogen application in the ear differentiation stage to compensate, represented by NDC867 and NDC918) variations. Both varieties presented the highest δ<sup>15</sup>N and <sup>15</sup>N abundances and Ndff (refers to the proportion of nitrogen in a plant's body that comes directly from the fertilizer applied.) in rice under the NDC treatment. Metagenomic sequencing of rhizospheric soil showed that the dominant bacterial groups at the phylum level among each treatment were Actinobacteria, Proteobacteria, Chloroflexi, Acidobacteria, Gemmatimonadetes, and Nitrospirae. The rhizosphere of indica rice was more enriched with the microbial communities involved in nitrogen metabolism, which contributed to higher nitrogen utilization efficiency. A correlation-based network was constructed and provides insights into the formation of nitrogen deficiency compensation effects and contributes to the enhancement of nitrogen uptake and utilization efficiency in rice production.</p>","PeriodicalId":20164,"journal":{"name":"Physiologia plantarum","volume":"176 5","pages":"e14549"},"PeriodicalIF":5.4,"publicationDate":"2024-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142372586","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Role of cotyledons in aluminium accumulation as a tolerance strategy in Fagopyrum esculentum Moench (Polygonaceae) seedlings. 子叶在 Fagopyrum esculentum Moench(蓼科)幼苗耐铝积累中的作用。
IF 5.4 2区 生物学 Q1 PLANT SCIENCES Pub Date : 2024-09-01 DOI: 10.1111/ppl.14554
Verónica Rodríguez-Sánchez, Daniel Tapia-Maruri, Judith Márquez-Guzmán, Sonia Vázquez-Santana, Rocío Cruz-Ortega

Acidic soils have increased due to agricultural practices, climate factors, and the excessive use of nitrogen fertilizers to meet food demand. In these soils, aluminium (Al) is soluble and can be taken up by roots, but it is toxic to most plant species. Fagopyrum esculentum is able to adapt to acidic toxic aluminium conditions. Anatomical studies identifying novel potential cellular structures as sites of Al accumulation are currently lacking. This study provides an anatomical description of the cotyledons, revealing the presence of papillae and glandular trichomes at their margins. In seedlings treated with 100 μM Al, energy-dispersive x-ray spectroscopy (ESEM-EDS) analysis of the cotyledons revealed that the margin has the highest concentration of Al. The margin containing the epidermal papillae was subjected to laser microdissection, and Al was quantified using mass spectrometry with an inductively coupled plasma source ICP-MS and compared with the Al in the remaining leaf blades. The concentration of Al in the microdissected papillae was 3,460 mg Al kg-1 Dry Weight (DW), whereas the blades contained only 1,390 mg Al kg-1 DW. Moreover, histochemical tests for Al and total phenols in the epidermal papillae revealed that Al may be bound to phenolic compounds. Thus, this study demonstrated that the cotyledons of F. esculentum have epidermal papillae that can accumulate Al.

由于农业耕作方式、气候因素以及为满足粮食需求而过量使用氮肥,酸性土壤日益增多。在这些土壤中,铝(Al)是可溶的,可被根系吸收,但对大多数植物物种有毒。Fagopyrum esculentum 能够适应酸性毒铝条件。目前还缺乏解剖学研究来确定新的潜在细胞结构作为铝积累的部位。本研究对子叶进行了解剖学描述,揭示了子叶边缘乳突和腺毛的存在。在用 100 μM Al 处理的幼苗中,子叶的能量色散 X 射线光谱(ESEM-EDS)分析表明,边缘的 Al 浓度最高。对含有表皮乳头的边缘进行了激光显微切割,并使用电感耦合等离子体质谱 ICP-MS 对铝进行了定量,并与其余叶片中的铝进行了比较。经显微切割的乳头中的铝含量为 3,460 毫克 Al kg-1 干重(DW),而叶片中的铝含量仅为 1,390 毫克 Al kg-1 干重。此外,对表皮乳头中的铝和总酚进行的组织化学测试表明,铝可能与酚类化合物结合在一起。因此,这项研究表明,F. esculentum 子叶的表皮乳头可以积累铝。
{"title":"Role of cotyledons in aluminium accumulation as a tolerance strategy in Fagopyrum esculentum Moench (Polygonaceae) seedlings.","authors":"Verónica Rodríguez-Sánchez, Daniel Tapia-Maruri, Judith Márquez-Guzmán, Sonia Vázquez-Santana, Rocío Cruz-Ortega","doi":"10.1111/ppl.14554","DOIUrl":"https://doi.org/10.1111/ppl.14554","url":null,"abstract":"<p><p>Acidic soils have increased due to agricultural practices, climate factors, and the excessive use of nitrogen fertilizers to meet food demand. In these soils, aluminium (Al) is soluble and can be taken up by roots, but it is toxic to most plant species. Fagopyrum esculentum is able to adapt to acidic toxic aluminium conditions. Anatomical studies identifying novel potential cellular structures as sites of Al accumulation are currently lacking. This study provides an anatomical description of the cotyledons, revealing the presence of papillae and glandular trichomes at their margins. In seedlings treated with 100 μM Al, energy-dispersive x-ray spectroscopy (ESEM-EDS) analysis of the cotyledons revealed that the margin has the highest concentration of Al. The margin containing the epidermal papillae was subjected to laser microdissection, and Al was quantified using mass spectrometry with an inductively coupled plasma source ICP-MS and compared with the Al in the remaining leaf blades. The concentration of Al in the microdissected papillae was 3,460 mg Al kg<sup>-1</sup> Dry Weight (DW), whereas the blades contained only 1,390 mg Al kg<sup>-1</sup> DW. Moreover, histochemical tests for Al and total phenols in the epidermal papillae revealed that Al may be bound to phenolic compounds. Thus, this study demonstrated that the cotyledons of F. esculentum have epidermal papillae that can accumulate Al.</p>","PeriodicalId":20164,"journal":{"name":"Physiologia plantarum","volume":"176 5","pages":"e14554"},"PeriodicalIF":5.4,"publicationDate":"2024-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142372585","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Xylem embolism induced by freeze-thaw and drought are influenced by different anatomical traits in subtropical montane evergreen angiosperm trees. 冻融和干旱引起的木质部栓塞受亚热带山地常绿被子植物不同解剖特征的影响。
IF 5.4 2区 生物学 Q1 PLANT SCIENCES Pub Date : 2024-09-01 DOI: 10.1111/ppl.14567
Feng Zhang, Yi-Wen Liu, Jie Qin, Steven Jansen, Shi-Dan Zhu, Kun-Fang Cao

Subtropical evergreen broadleaved forests distributed in montane zones of southern China experience seasonal droughts and winter frost. Previously, studies have recognized that xylem anatomy is a determinant of its vulnerability to embolism caused by drought and freezing events. We hypothesized that there is a coordination of xylem resistance to freeze-thaw and drought-induced embolism for the subtropical montane evergreen broadleaved tree species because they are influenced by common xylem structural traits (e.g., vessel diameter). We examined the branch xylem anatomy, resistance to drought-induced embolism (P50), and the percent loss of branch hydraulic conductivity after a severe winter frost (PLCwinter) for 15 evergreen broadleaved tree species in a montane forest in South China. Our results showed that P50 of the studied species ranged from -2.81 to -5.13 MPa, which was not associated with most xylem anatomical properties except for the axial parenchyma-to-vessel connectivity. These tree species differed substantially in PLCwinter, ranging from 0% to 76.41%. PLCwinter was positively related to vessel diameter and negatively related to vessel density, vessel group index, and vessel-to-vessel connectivity, but no coordination with P50. This study suggests that hydraulic adaptation to frost is important to determine the distributional limit of subtropical montane evergreen woody angiosperms.

分布在中国南方山地地区的亚热带常绿阔叶林会经历季节性干旱和冬季霜冻。以前的研究已经认识到木质部的解剖结构决定了它对干旱和冰冻事件引起的栓塞的脆弱性。我们假设,亚热带山地常绿阔叶树种木质部对冻融和干旱引起的栓塞的抗性是相互协调的,因为它们受到共同的木质部结构特征(如血管直径)的影响。我们研究了华南山地森林中 15 个常绿阔叶树种的枝条木质部解剖结构、抗旱栓塞能力(P50)以及严冬霜冻(PLCwinter)后枝条导水性损失的百分比。结果表明,所研究树种的P50介于-2.81至-5.13 MPa之间,除了轴向实质与血管的连通性外,与大多数木质部解剖特性无关。这些树种在 PLCwinter 方面差异很大,从 0% 到 76.41% 不等。PLCwinter 与血管直径呈正相关,与血管密度、血管组指数和血管间连接呈负相关,但与 P50 无关。这项研究表明,水力对霜冻的适应性对于确定亚热带山地常绿木本被子植物的分布界限非常重要。
{"title":"Xylem embolism induced by freeze-thaw and drought are influenced by different anatomical traits in subtropical montane evergreen angiosperm trees.","authors":"Feng Zhang, Yi-Wen Liu, Jie Qin, Steven Jansen, Shi-Dan Zhu, Kun-Fang Cao","doi":"10.1111/ppl.14567","DOIUrl":"https://doi.org/10.1111/ppl.14567","url":null,"abstract":"<p><p>Subtropical evergreen broadleaved forests distributed in montane zones of southern China experience seasonal droughts and winter frost. Previously, studies have recognized that xylem anatomy is a determinant of its vulnerability to embolism caused by drought and freezing events. We hypothesized that there is a coordination of xylem resistance to freeze-thaw and drought-induced embolism for the subtropical montane evergreen broadleaved tree species because they are influenced by common xylem structural traits (e.g., vessel diameter). We examined the branch xylem anatomy, resistance to drought-induced embolism (P<sub>50</sub>), and the percent loss of branch hydraulic conductivity after a severe winter frost (PLC<sub>winter</sub>) for 15 evergreen broadleaved tree species in a montane forest in South China. Our results showed that P<sub>50</sub> of the studied species ranged from -2.81 to -5.13 MPa, which was not associated with most xylem anatomical properties except for the axial parenchyma-to-vessel connectivity. These tree species differed substantially in PLC<sub>winter</sub>, ranging from 0% to 76.41%. PLC<sub>winter</sub> was positively related to vessel diameter and negatively related to vessel density, vessel group index, and vessel-to-vessel connectivity, but no coordination with P<sub>50</sub>. This study suggests that hydraulic adaptation to frost is important to determine the distributional limit of subtropical montane evergreen woody angiosperms.</p>","PeriodicalId":20164,"journal":{"name":"Physiologia plantarum","volume":"176 5","pages":"e14567"},"PeriodicalIF":5.4,"publicationDate":"2024-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142392506","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
HAIRY MERISTEM proteins regulate the WUSCHEL protein levels in mediating CLAVATA3 expression. HAIRY MERISTEM 蛋白在介导 CLAVATA3 表达的过程中调节 WUSCHEL 蛋白水平。
IF 5.4 2区 生物学 Q1 PLANT SCIENCES Pub Date : 2024-09-01 DOI: 10.1111/ppl.14505
Kevin Rodriguez, Lloyd Kao, Vincent E Cerbantez-Bueno, Christian Delgadillo, Dorothy Nguyen, Samin Ullah, Cameron Delgadillo, G Venugopala Reddy

The precise regulation of stem cells in the shoot apical meristems (SAMs) involves the function of the homeodomain transcription factor (TF)-WUSCHEL (WUS). WUS has been shown to move from the site of production-the rib-meristem (RM), into overlaying cells of the central zone (CZ), where it specifies stem cells and also regulates the transcription of CLAVATA3 (CLV3). The secreted signalling peptide CLV3 activates a receptor kinase signalling that restricts WUS transcription and also regulates the nuclear gradient of WUS by offsetting nuclear export. WUS has been shown to regulate both CLV3 levels and spatial activation, restricting its expression to a few cells in the CZ. The HAIRY MERISTEM (HAM), a GRASS-domain class of TFs expressed in the RM, has been shown to physically interact with WUS and regulate CLV3 expression. However, the mechanisms by which this interaction regulates CLV3 expression non-cell autonomously remain unclear. Here, we show that HAM function is required for regulating the WUS protein stability, and the CLV3 expression responds to altered WUS protein levels in ham mutants. Thus, HAM proteins non-cell autonomously regulates CLV3 expression.

对嫩枝顶端分生组织(SAM)中干细胞的精确调控涉及同源转录因子(TF)-WUSCHEL(WUS)的功能。研究表明,WUS会从产生部位--肋-分生组织(RM)移动到中央区(CZ)的重叠细胞中,在那里指定干细胞,并调节CLAVATA3(CLV3)的转录。分泌的信号肽CLV3会激活受体激酶信号,从而限制WUS的转录,并通过抵消核输出来调节WUS的核梯度。研究表明,WUS 可调节 CLV3 的水平和空间激活,将其表达限制在 CZ 中的少数细胞内。HAIRY MERISTEM(HAM)是在RM中表达的一类GRASS-domain TFs,已被证明能与WUS发生物理相互作用并调节CLV3的表达。然而,这种相互作用非细胞自主调节 CLV3 表达的机制仍不清楚。在这里,我们发现 HAM 的功能是调节 WUS 蛋白稳定性所必需的,而且在 ham 突变体中,CLV3 的表达会对 WUS 蛋白水平的改变做出反应。因此,HAM蛋白非细胞自主调节CLV3的表达。
{"title":"HAIRY MERISTEM proteins regulate the WUSCHEL protein levels in mediating CLAVATA3 expression.","authors":"Kevin Rodriguez, Lloyd Kao, Vincent E Cerbantez-Bueno, Christian Delgadillo, Dorothy Nguyen, Samin Ullah, Cameron Delgadillo, G Venugopala Reddy","doi":"10.1111/ppl.14505","DOIUrl":"10.1111/ppl.14505","url":null,"abstract":"<p><p>The precise regulation of stem cells in the shoot apical meristems (SAMs) involves the function of the homeodomain transcription factor (TF)-WUSCHEL (WUS). WUS has been shown to move from the site of production-the rib-meristem (RM), into overlaying cells of the central zone (CZ), where it specifies stem cells and also regulates the transcription of CLAVATA3 (CLV3). The secreted signalling peptide CLV3 activates a receptor kinase signalling that restricts WUS transcription and also regulates the nuclear gradient of WUS by offsetting nuclear export. WUS has been shown to regulate both CLV3 levels and spatial activation, restricting its expression to a few cells in the CZ. The HAIRY MERISTEM (HAM), a GRASS-domain class of TFs expressed in the RM, has been shown to physically interact with WUS and regulate CLV3 expression. However, the mechanisms by which this interaction regulates CLV3 expression non-cell autonomously remain unclear. Here, we show that HAM function is required for regulating the WUS protein stability, and the CLV3 expression responds to altered WUS protein levels in ham mutants. Thus, HAM proteins non-cell autonomously regulates CLV3 expression.</p>","PeriodicalId":20164,"journal":{"name":"Physiologia plantarum","volume":"176 5","pages":"e14505"},"PeriodicalIF":5.4,"publicationDate":"2024-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142110904","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Ultraviolet-induced melanisation in lichens: physiological traits and transcriptome profile. 紫外线诱导的地衣黑化:生理特征和转录组特征。
IF 5.4 2区 生物学 Q1 PLANT SCIENCES Pub Date : 2024-09-01 DOI: 10.1111/ppl.14512
Ilya Leksin, Mikhail Shelyakin, Ilya Zakhozhiy, Olga Kozlova, Richard Beckett, Farida Minibayeva

Lichens are important components of high-latitude boreal and Arctic habitats. While stress tolerant, they are among the most sensitive ecosystem components to climate change, in particular, an increase in ultraviolet light (UV) arising from polar ozone depletion and deforestation. This study is the first to explore the effects of UV-B on gene expression in lichens to predict metabolic pathways involved in tolerance. Using transcriptome profiling and bioinformatic analyses, here we studied the effects of UV-B on gene expression in lichens using Lobaria pulmonaria (L.) Hoff. as a model species. UV-B exposure causes significant browning of the upper cortex of the thallus, which correlates to an increased expression of biosynthetic gene clusters involved in the synthesis of eu- and allomelanins and melanin precursors. Based on transcriptome analyses, we suggest that the biosynthesis of melanins and other secondary metabolites, such as naphthalene derivates, tropolones, anthraquinones, and xanthones, is a trade-off that lichens pay to protect essential metabolic processes such as photosynthesis and respiration. Expression profiles of general stress-associated genes, in particular, related to reactive oxygen species scavenging, protection of proteins, and DNA repair, clearly indicate that the mycobiont is the more UV-B-responsive and susceptible partner in lichen symbiosis. Our findings demonstrate that UV-B stress activates an intricate gene network involved in tolerance mechanisms of lichen symbionts. Knowledge obtained here may enable the prediction of likely effects on lichen biodiversity caused by climate change and pollution.

地衣是高纬度北方和北极栖息地的重要组成部分。虽然地衣耐受压力,但它们是对气候变化最敏感的生态系统组成部分之一,特别是极地臭氧消耗和森林砍伐导致的紫外线(UV)增加。本研究首次探讨了紫外线-B 对地衣基因表达的影响,以预测地衣耐受性所涉及的代谢途径。利用转录组分析和生物信息学分析,我们以 Lobaria pulmonaria (L.) Hoff.为模式物种,研究了紫外线-B 对地衣基因表达的影响。紫外线-B照射会导致苔藓上部皮层明显褐化,这与参与合成黑色素和异黑色素以及黑色素前体的生物合成基因簇的表达增加有关。根据转录组分析,我们认为黑色素和其他次生代谢物(如萘衍生物、萘酮类、蒽醌类和黄酮类)的生物合成是地衣为保护光合作用和呼吸作用等基本代谢过程而付出的代价。一般应激相关基因的表达谱,特别是与活性氧清除、蛋白质保护和 DNA 修复有关的基因的表达谱,清楚地表明在地衣共生中,菌丝体是对紫外线-B 反应更强、更易受影响的伙伴。我们的研究结果表明,紫外线-B 胁迫激活了地衣共生体耐受机制中错综复杂的基因网络。这些知识有助于预测气候变化和污染可能对地衣生物多样性造成的影响。
{"title":"Ultraviolet-induced melanisation in lichens: physiological traits and transcriptome profile.","authors":"Ilya Leksin, Mikhail Shelyakin, Ilya Zakhozhiy, Olga Kozlova, Richard Beckett, Farida Minibayeva","doi":"10.1111/ppl.14512","DOIUrl":"https://doi.org/10.1111/ppl.14512","url":null,"abstract":"<p><p>Lichens are important components of high-latitude boreal and Arctic habitats. While stress tolerant, they are among the most sensitive ecosystem components to climate change, in particular, an increase in ultraviolet light (UV) arising from polar ozone depletion and deforestation. This study is the first to explore the effects of UV-B on gene expression in lichens to predict metabolic pathways involved in tolerance. Using transcriptome profiling and bioinformatic analyses, here we studied the effects of UV-B on gene expression in lichens using Lobaria pulmonaria (L.) Hoff. as a model species. UV-B exposure causes significant browning of the upper cortex of the thallus, which correlates to an increased expression of biosynthetic gene clusters involved in the synthesis of eu- and allomelanins and melanin precursors. Based on transcriptome analyses, we suggest that the biosynthesis of melanins and other secondary metabolites, such as naphthalene derivates, tropolones, anthraquinones, and xanthones, is a trade-off that lichens pay to protect essential metabolic processes such as photosynthesis and respiration. Expression profiles of general stress-associated genes, in particular, related to reactive oxygen species scavenging, protection of proteins, and DNA repair, clearly indicate that the mycobiont is the more UV-B-responsive and susceptible partner in lichen symbiosis. Our findings demonstrate that UV-B stress activates an intricate gene network involved in tolerance mechanisms of lichen symbionts. Knowledge obtained here may enable the prediction of likely effects on lichen biodiversity caused by climate change and pollution.</p>","PeriodicalId":20164,"journal":{"name":"Physiologia plantarum","volume":"176 5","pages":"e14512"},"PeriodicalIF":5.4,"publicationDate":"2024-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142110908","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
A bHLH transcription factor AaMYC2-type positively regulates glandular trichome density and artemisinin biosynthesis in Artemisia annua. 一种 bHLH 转录因子 AaMYC2- 型可正向调节黄花蒿的腺毛密度和青蒿素生物合成。
IF 5.4 2区 生物学 Q1 PLANT SCIENCES Pub Date : 2024-09-01 DOI: 10.1111/ppl.14581
Rameez Ahmad Khan, Amit Kumar, Nazia Abbas

Artemisinin-based combinational therapies (ACTs) constitute the first line of malaria treatment. However, due to its trichome-specific biosynthesis, low concentration, and poor understanding of regulatory mechanisms involved in artemisinin biosynthesis and trichome development, it becomes very difficult to meet the increased demand for ACTs. Here, we have reported that a bHLH transcription factor, AaMYC2-type, plays an important role in regulating GST development and artemisinin biosynthesis in Artemisia annua. AaMYC2-type encodes a protein that is transcriptionally active and localised to the nucleus. It is prominently expressed in aerial parts like leaves, stems, inflorescence and least expressed in roots. AaMYC2-type expression is significantly increased under different hormonal treatments. In transgenic overexpression lines, AaMYC2-type OE, a significant increase in the expression of trichome development and artemisinin biosynthesis genes was observed. While in knockdown lines, Aamyc2-type, expression of trichome development and artemisinin biosynthesis genes were significantly reduced. Yeast one-hybrid assay clearly shows that the AaMYC2-type directly binds to the E-boxes in the promoter regions of ADS and CYP71AVI. The SEM microscopy depicted the number of trichomes elevated from 11 mm-2 in AaMYC2-type OE lines to 6.1 mm-2 in Aamyc2-type. The final effect of the alteration in biosynthetic and trichome developmental genes was observed in the accumulation of artemisinin. In the AaMYC2-type OE, the artemisinin content was 12 mg g-1DW, which was reduced to 3.2 mg g-1DW in the Aamyc2-type. Altogether, the above findings suggest that the AaMYC2-type play a dual regulating role in controlling both trichome developmental and artemisinin biosynthetic genes.

青蒿素类复方疗法(ACTs)是治疗疟疾的第一线药物。然而,由于青蒿素的毛状体特异性生物合成、低浓度以及对青蒿素生物合成和毛状体发育的调控机制了解甚少,因此很难满足对 ACTs 日益增长的需求。在此,我们报告了一种 bHLH 转录因子 AaMYC2- 型在调控黄花蒿 GST 发育和青蒿素生物合成中的重要作用。AaMYC2- 型编码一种具有转录活性并定位在细胞核中的蛋白质。它主要在叶、茎、花序等气生部分表达,在根中表达最少。在不同的激素处理下,AaMYC2-型的表达量明显增加。在转基因过表达系 AaMYC2 型 OE 中,观察到毛状体发育基因和青蒿素生物合成基因的表达明显增加。而在 Aamyc2- 型基因敲除株系中,毛状体发育基因和青蒿素生物合成基因的表达量明显降低。酵母单杂交试验清楚地表明,AaMYC2-型直接与 ADS 和 CYP71AVI 启动子区域的 E-boxes 结合。SEM 显微镜显示,毛状体的数量从 AaMYC2- 型 OE 株系的 11 mm-2 增加到 Aamyc2- 型的 6.1 mm-2。生物合成基因和毛状体发育基因改变的最终影响体现在青蒿素的积累上。在 AaMYC2-型 OE 中,青蒿素含量为 12 mg g-1DW,而在 Aamyc2-型中,青蒿素含量降至 3.2 mg g-1DW。总之,上述研究结果表明,AaMYC2-型在控制毛状体发育和青蒿素生物合成基因方面起着双重调控作用。
{"title":"A bHLH transcription factor AaMYC2-type positively regulates glandular trichome density and artemisinin biosynthesis in Artemisia annua.","authors":"Rameez Ahmad Khan, Amit Kumar, Nazia Abbas","doi":"10.1111/ppl.14581","DOIUrl":"https://doi.org/10.1111/ppl.14581","url":null,"abstract":"<p><p>Artemisinin-based combinational therapies (ACTs) constitute the first line of malaria treatment. However, due to its trichome-specific biosynthesis, low concentration, and poor understanding of regulatory mechanisms involved in artemisinin biosynthesis and trichome development, it becomes very difficult to meet the increased demand for ACTs. Here, we have reported that a bHLH transcription factor, AaMYC2-type, plays an important role in regulating GST development and artemisinin biosynthesis in Artemisia annua. AaMYC2-type encodes a protein that is transcriptionally active and localised to the nucleus. It is prominently expressed in aerial parts like leaves, stems, inflorescence and least expressed in roots. AaMYC2-type expression is significantly increased under different hormonal treatments. In transgenic overexpression lines, AaMYC2-type OE, a significant increase in the expression of trichome development and artemisinin biosynthesis genes was observed. While in knockdown lines, Aamyc2-type, expression of trichome development and artemisinin biosynthesis genes were significantly reduced. Yeast one-hybrid assay clearly shows that the AaMYC2-type directly binds to the E-boxes in the promoter regions of ADS and CYP71AVI. The SEM microscopy depicted the number of trichomes elevated from 11 mm<sup>-2</sup> in AaMYC2-type OE lines to 6.1 mm<sup>-2</sup> in Aamyc2-type. The final effect of the alteration in biosynthetic and trichome developmental genes was observed in the accumulation of artemisinin. In the AaMYC2-type OE, the artemisinin content was 12 mg g<sup>-1</sup>DW, which was reduced to 3.2 mg g<sup>-1</sup>DW in the Aamyc2-type. Altogether, the above findings suggest that the AaMYC2-type play a dual regulating role in controlling both trichome developmental and artemisinin biosynthetic genes.</p>","PeriodicalId":20164,"journal":{"name":"Physiologia plantarum","volume":"176 5","pages":"e14581"},"PeriodicalIF":5.4,"publicationDate":"2024-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142505936","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
SNP-based QTL mapping and identification of panicle structure-related genes in rice. 基于SNP的QTL图谱和水稻圆锥花序结构相关基因的鉴定
IF 5.4 2区 生物学 Q1 PLANT SCIENCES Pub Date : 2024-09-01 DOI: 10.1111/ppl.14588
Jae-Ryoung Park, Jeonghwan Seo, Chang-Min Lee, O-Young Jeong, Mina Jin, Songhee Park, Hyun-Su Park

Rice is a staple crop providing a significant portion of the global food supply. It is then crucial to develop strategies for breeding high-yield cultivars to meet global food security challenges, including the UN's zero-hunger goal. In this study, QTL mapping was employed to pinpoint key genomic regions linked to traits influencing rice yield, with a focus on panicle structure-a critical determinant of grain number. Over two consecutive years, QTLs were identified using 88 JJ625LG/Namchan Recombinant Inbred Lines (JNRILs), revealing several candidate genes. Notably, Gn1a, a known regulator of grain number, was mapped within qNS1 and qNSSr1-1, while the sd1 gene, linked to plant height, was detected across multiple QTLs. Furthermore, a novel gene, OsNSMq3 (Os03g0843800), encoding a methyltransferase, was identified in various QTLs, with haplotype and sequence homology analysis suggesting its role in enhancing yield by influencing panicle structure development. The increase in primary and secondary branches, driven by these genes, leads to a higher number of spikelets per panicle, thereby boosting yield. These findings underscore the potential of candidate genes from stable QTLs as valuable tools in molecular breeding to develop high-yield rice cultivars, addressing global hunger and aiding food supply in refugee crises.

水稻是一种主粮作物,在全球粮食供应中占有重要地位。因此,制定培育高产栽培品种的战略以应对全球粮食安全挑战,包括实现联合国的零饥饿目标至关重要。在这项研究中,我们利用 QTL 图谱确定了与影响水稻产量的性状相关联的关键基因组区域,重点是圆锥花序结构--粒数的关键决定因素。连续两年,利用 88 个 JJ625LG/Namchan 重组近交系(JNRILs)鉴定了 QTLs,发现了几个候选基因。值得注意的是,谷粒数的已知调节基因 Gn1a 被映射到 qNS1 和 qNSSr1-1 中,而与株高有关的 sd1 基因则在多个 QTL 中被检测到。此外,在多个 QTL 中还发现了一个编码甲基转移酶的新基因 OsNSMq3(Os03g0843800),单倍型和序列同源性分析表明,该基因通过影响圆锥花序结构的发育来提高产量。在这些基因的驱动下,主枝和副主枝的增加导致每个圆锥花序的小穗数量增加,从而提高了产量。这些发现强调了稳定 QTL 的候选基因作为分子育种宝贵工具的潜力,可用于开发高产水稻栽培品种,解决全球饥饿问题并帮助难民危机中的粮食供应。
{"title":"SNP-based QTL mapping and identification of panicle structure-related genes in rice.","authors":"Jae-Ryoung Park, Jeonghwan Seo, Chang-Min Lee, O-Young Jeong, Mina Jin, Songhee Park, Hyun-Su Park","doi":"10.1111/ppl.14588","DOIUrl":"https://doi.org/10.1111/ppl.14588","url":null,"abstract":"<p><p>Rice is a staple crop providing a significant portion of the global food supply. It is then crucial to develop strategies for breeding high-yield cultivars to meet global food security challenges, including the UN's zero-hunger goal. In this study, QTL mapping was employed to pinpoint key genomic regions linked to traits influencing rice yield, with a focus on panicle structure-a critical determinant of grain number. Over two consecutive years, QTLs were identified using 88 JJ625LG/Namchan Recombinant Inbred Lines (JNRILs), revealing several candidate genes. Notably, Gn1a, a known regulator of grain number, was mapped within qNS1 and qNSSr1-1, while the sd1 gene, linked to plant height, was detected across multiple QTLs. Furthermore, a novel gene, OsNSMq3 (Os03g0843800), encoding a methyltransferase, was identified in various QTLs, with haplotype and sequence homology analysis suggesting its role in enhancing yield by influencing panicle structure development. The increase in primary and secondary branches, driven by these genes, leads to a higher number of spikelets per panicle, thereby boosting yield. These findings underscore the potential of candidate genes from stable QTLs as valuable tools in molecular breeding to develop high-yield rice cultivars, addressing global hunger and aiding food supply in refugee crises.</p>","PeriodicalId":20164,"journal":{"name":"Physiologia plantarum","volume":"176 5","pages":"e14588"},"PeriodicalIF":5.4,"publicationDate":"2024-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142505938","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
期刊
Physiologia plantarum
全部 Acc. Chem. Res. ACS Applied Bio Materials ACS Appl. Electron. Mater. ACS Appl. Energy Mater. ACS Appl. Mater. Interfaces ACS Appl. Nano Mater. ACS Appl. Polym. Mater. ACS BIOMATER-SCI ENG ACS Catal. ACS Cent. Sci. ACS Chem. Biol. ACS Chemical Health & Safety ACS Chem. Neurosci. ACS Comb. Sci. ACS Earth Space Chem. ACS Energy Lett. ACS Infect. Dis. ACS Macro Lett. ACS Mater. Lett. ACS Med. Chem. Lett. ACS Nano ACS Omega ACS Photonics ACS Sens. ACS Sustainable Chem. Eng. ACS Synth. Biol. Anal. Chem. BIOCHEMISTRY-US Bioconjugate Chem. BIOMACROMOLECULES Chem. Res. Toxicol. Chem. Rev. Chem. Mater. CRYST GROWTH DES ENERG FUEL Environ. Sci. Technol. Environ. Sci. Technol. Lett. Eur. J. Inorg. Chem. IND ENG CHEM RES Inorg. Chem. J. Agric. Food. Chem. J. Chem. Eng. Data J. Chem. Educ. J. Chem. Inf. Model. J. Chem. Theory Comput. J. Med. Chem. J. Nat. Prod. J PROTEOME RES J. Am. Chem. Soc. LANGMUIR MACROMOLECULES Mol. Pharmaceutics Nano Lett. Org. Lett. ORG PROCESS RES DEV ORGANOMETALLICS J. Org. Chem. J. Phys. Chem. J. Phys. Chem. A J. Phys. Chem. B J. Phys. Chem. C J. Phys. Chem. Lett. Analyst Anal. Methods Biomater. Sci. Catal. Sci. Technol. Chem. Commun. Chem. Soc. Rev. CHEM EDUC RES PRACT CRYSTENGCOMM Dalton Trans. Energy Environ. Sci. ENVIRON SCI-NANO ENVIRON SCI-PROC IMP ENVIRON SCI-WAT RES Faraday Discuss. Food Funct. Green Chem. Inorg. Chem. Front. Integr. Biol. J. Anal. At. Spectrom. J. Mater. Chem. A J. Mater. Chem. B J. Mater. Chem. C Lab Chip Mater. Chem. Front. Mater. Horiz. MEDCHEMCOMM Metallomics Mol. Biosyst. Mol. Syst. Des. Eng. Nanoscale Nanoscale Horiz. Nat. Prod. Rep. New J. Chem. Org. Biomol. Chem. Org. Chem. Front. PHOTOCH PHOTOBIO SCI PCCP Polym. Chem.
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
0
微信
客服QQ
Book学术公众号 扫码关注我们
反馈
×
意见反馈
请填写您的意见或建议
请填写您的手机或邮箱
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
现在去查看 取消
×
提示
确定
Book学术官方微信
Book学术文献互助
Book学术文献互助群
群 号:481959085
Book学术
文献互助 智能选刊 最新文献 互助须知 联系我们:info@booksci.cn
Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。
Copyright © 2023 Book学术 All rights reserved.
ghs 京公网安备 11010802042870号 京ICP备2023020795号-1