PLoS ONE最新文献

Background: Acute kidney injury (AKI) is still a serious kidney illness with high morbidity and death rates, and it's crucial to comprehend the underlying molecular causes.

Methods: Bioinformatics analysis was performed on GSE139061 and GSE30718 data sets, and COX7A2L was screened out. The role of COX7A2L in H/R-treated cells and its transcriptional regulation with TCF4 were assessed. In vitro experiments analyzed the regulation of COX7A2L and TCF4 on the proliferation, apoptosis, and Wnt/β-catenin signaling pathway of H/R-treated cells.

Results: COX7A2L as a hub gene was downregulated in AKI samples. In H/R-treated cells, COX7A2L overexpression inhibited apoptosis and promoted cell proliferation, while COX7A2L knockdown promoted apoptosis and inhibited cell proliferation. Notably, TCF4 exhibited a significant positive correlation with COX7A2L. TCF4 overexpression-induced apoptosis was lessened and improved cell proliferation was countered by COX7A2L knockdown, according to rescue study findings. Besides, we discovered that TCF4 overexpression increased the expression of proteins linked to the Wnt/β-catenin signaling pathway (c-myc, β-catenin, and cyclin D1), while underexpression of COX7A2L counteracted this effect.

Conclusion: The study revealed the pivotal role of COX7A2L in AKI, which is regulated by TCF4 and modulates the Wnt/β-catenin signaling pathway, highlighting its potential as a therapeutic target.

Knowledge on the variation of yield and water use efficiency under different mulching methods is important for guiding rained maize production in the Loess Plateau area. In this study, eight different plastic film mulching methods was established to analyze the maize growth, soil water content and soil temperature changes of dryland maize, and increase yield and water use efficiency (WUE). The field experiment was conducted in 2019, and eight treatments were set up, including a traditional flat planting without mulching (CK), ridge-furrow with ridges mulching black plastic film and furrows mulching straw (HJ), ridge-furrow with ridges mulching black plastic film and furrows bare (HL), ridge-furrow with ridges mulching liquid plastic film and furrows mulching straw (YJ), ridge-furrow with ridges mulching liquid plastic film and furrows bare (YL), ridge-furrow with ridges mulching biodegradable plastic film and furrows mulching straw (SJ), ridge-furrow with ridges mulching biodegradable plastic film and furrows bare (SL) and ridge-furrow with ridges bare and furrows mulching straw (NJ). Furthermore, the AHP-TOPSIS was employed to evaluate the optimal mulching method for maize. The results showed that compared with CK and NJ treatment, the soil water content and soil storage were significantly changes with other treatments in the reproductive period of maize. Among the six mulching methods, maize yield in HJ, HL, YJ, YL, SJ, and SL treatments were 46.28%, 61.95%, 70.30%, 51.02%, 52.02% and 53.53% significantly greater than CK treatment. In addition, dryland maize WUE was 66.53% and 84.01% higher in the YJ and YL treatments with ridges mulching liquid plastic film than in the CK treatment, respectively. The optimal treatments of economic benefits were YL and HJ. Through AHP-TOPSIS comprehensive analysis, the optimal mulching methods were YL and HJ treatment. Current field trials indicate that YL treatment could serve as a promising option to improve dryland maize yield, WUE, and reducing environmental risks in the Loess Plateau of China.

Rapid acceleration is an important quality for field-based sport athletes. Technical factors contribute to acceleration and these can be deliberately influenced by coaches through implementation of constraints, which afford particular coordinative states or induce variability generally. Lightweight wearable resistance is an emerging training tool, which can act as a constraint on acceleration. At present, however, the effects on whole body coordination resulting from wearable resistance application are unknown. To better understand these effects, five male Australian Rules football athletes performed a series of 20 m sprints with either relatively light or heavy wearable resistance applied to the anterior or posterior aspects of the thighs or shanks. Whole body coordination during early acceleration was examined across eight wearable resistance conditions and compared with baseline (unresisted) acceleration coordination using group- and individual-level hierarchical cluster analysis. Self-organising maps and a joint-level distance matrix were used to further investigate specific kinematic changes in conditions where coordination differed most from baseline. Across the group, relatively heavy wearable resistance applied to the thighs resulted in the greatest difference to whole body coordination compared with baseline acceleration. On average, heavy posterior thigh wearable resistance led to altered pelvic position and greater hip extension, while heavy anterior thigh wearable resistance led to accentuated movement at the shoulders in the transverse and sagittal planes. These findings offer a useful starting point for coaches seeking to use wearable resistance to promote adoption of greater hip extension or upper body contribution during acceleration. Importantly, individuals varied in how they responded to heavy thigh wearable resistance, which coaches should be mindful of.

Cassava is a staple in the diet of millions of people in sub-Saharan Africa, as it can grow in poor soils with limited inputs and can withstand a wide range of environmental conditions, including drought. Previous studies have shown that the distribution of rural populations is an important predictor of cassava density in sub-Saharan Africa's landscape. Our aim is to explore relationships between the distribution of cassava from the cassava production disaggregation models (CassavaMap and MapSPAM) and rural population density, looking at potential differences between countries and regions. We analysed various properties of cassava cultivations collected from surveys at 69 locations in Côte d'Ivoire and 87 locations in Uganda conducted between February and March 2018. The relationships between the proportion of surveyed land under cassava cultivation and rural population and settlement data were examined using a set of generalized additive models within each country. Information on rural settlements was aggregated around the survey locations at 2, 5 and 10 km circular buffers. The analysis of the original survey data showed no significant correlation between rural population and cassava production in both MapSPAM and CassavaMap. However, as we aggregate settlement buffers around the survey locations using CassavaMap, we find that at a large scale this model does capture large-scale variations in cassava production. Moreover, through our analyses, we discovered country-specific spatial trends linked to areas of higher cassava production. These analyses are useful for validating disaggregation models of cassava production. As the certainty that existing cassava production maps increases, analyses that rely on the disaggregation maps, such as models of disease spread, nutrient availability from cassava with respect to population in a region, etc. can be performed with increased confidence. These benefit social and natural scientists, policymakers and the population in general by ensuring that cassava production estimates are increasingly reliable.

Background: Eupatilin, a flavone isolated from Artemisia species, exerts anti-inflammatory, anti-oxidative, and anti-neoplastic activities. However, the effects of eupatilin on H. pylori-associated gastritis remain unclear. Thus, this study aimed to investigate the anti-inflammatory effects of eupatilin on gastric epithelial cells infected with cytotoxin-associated gene A (CagA)-positive Helicobacter pylori.

Materials and methods: AGS human gastric carcinoma cells were infected with a CagA-positive H. pylori strain and then treated with 10, 50, or 100 ng of eupatilin. After 24 h, the expression levels of CagA, phosphoinositide 3-kinase 1 (PI3K), nuclear factor (NF)-κB, interleukin (IL)-1β, and tumor necrosis factor (TNF)-α in the cell lysates were measured using western blotting, and the mRNA levels of IL-6, IL-8, and monocyte chemoattractant protein (MCP)-1 were measured using real-time polymerase chain reaction.

Results: CagA translocation into AGS cells resulted in an elongated cell morphology, which was significantly suppressed by eupatilin treatment in a dose-dependent manner. Immunofluorescence staining for anti-CagA showed that eupatilin treatment dose-dependently inhibited CagA expression in the H. pylori-infected AGS cells. H. pylori infection increased the levels of pro-inflammatory cytokines including IL-1β, TNF-α, IL-6, IL-8, and MCP-1, and eupatilin treatment significantly reduced the levels of these cytokines in a dose-dependent manner. Additionally, eupatilin treatment dose-dependently suppressed the expression of PI3K and NF-κB.

Conclusions: Eupatilin treatment demonstrated anti-inflammatory effects on CagA-positive H. pylori-infected gastric epithelial cells by inhibiting CagA translocation, thereby suppressing the NF-κB signaling pathway. These results suggest that eupatilin plays a protective role against CagA-positive H. pylori-induced gastritis.

Purpose: Distal radius fractures (DRF) that occur from a fall from level ground are considered fragility fractures and may be the first indication that someone has compromised bone mineral density and is at risk of having osteoporosis. Women at about 50 years of age experience a dramatic increase risk of these fractures. Rehabilitation after DRF focuses on restoring range of motion and strength in the wrist, but rarely address future bone health concerns. We developed a 6-week therapist-developed remote full body exercise and osteoporosis/bone-health education program (called The Hands Up Program). This study was designed to evaluate the feasibility of a larger trial examining the effects of a whole-body exercise and education program for people 50-65 after a DRF.

Methods: Community dwelling individuals between the age of 50-65 with a radiographically confirmed DRF were recruited from the Roth| McFarlane Hand and Upper Limb Center in London, Ontario. Participants were randomized in a 1:1 ratio into either the Hands Up Program which was a twice weekly exercise and education program for 6 weeks, or the control group where they proceeded with usual care. The intervention was delivered online through a website where participants created a unique username and password to access. The primary feasibility outcomes of the study were recruitment rate (74 participants in 1 year), retention rate (75% completion), and intervention adherence rate (60% of completion of the exercise program). Secondary outcomes included strength, range of motion, self-reported outcomes, and bone density.

Results: Overall, 74 participants were recruited in 14 months. Retention did not meet the criteria for success, as only 53% of the participants attended their 12-month visit. Adherence was also not met albeit close with 55% completing the exercise program. Twelve participants withdrew from the study, five due to the time commitment, four without explanation, one due to group allocation, one due to COVID and one participant moved. One participant was deemed ineligible after consent. Four of the participants that withdrew were in the intervention group, and four in the control group, five participants withdrew before they were allocated to a group. Feedback from patients indicated potential improvements to the program: lower assessment burden, spacing out the intervention so that the education portion could be delivered during the immobilization phase of recovery, and creating a more individualized program.

Conclusion: Adherence and retention were both a challenge, although adherence was close to target. While achieving adherence to exercise in bone health is a known challenge, given the importance of prevention in the at-risk DRF population and the challenges in co-design and delivery during the pandemic, we believe evaluation of a revitalized program is warranted.

Coix lacryma-jobi L. belongs to family Poaceae, is widely cultivated in tropical Asian countries for its nutritional and medicinal values. Coix is often threatened by lepidopteran such as Mythimna separata during its life cycle, resulting in severe yield reduction. Insect feeding can trigger defense signaling and increased defense responses in many other crops, yet little is known about whether simulated armyworm feeding on Coix leaves could induce anti-herbivory responses and whether armyworm feeding could activate priming in systemic leaves. In this study, Mythimna separata simulated herbivory elicited increased jasmonic acid (JA) level, JA-Ile (JA-isoleucine conjugate) and altered transcriptome in the Coix leaves. Meanwhile, M. separata simulated herbivory in local leaves primed the systemic leaves for increased accumulation of jasmonic acid and enhanced resistance to M. separata. Consistently, transcriptome analysis showed the systemic leaves were primed, which were up- or down-regulated comparing with the non-primed systemic leaves. In this study, we first reported Mythimna separata simulated herbivory induced increased defense response in leaves of Coix, also Mythimna separata herbivory primed Coix resistance in systemic leaves. This study provides new insight into the regulation of defense responses of Coix against M. separata and the ecological function of priming in Coix.

Behavioral perception-action dissociations are widely used to test models of high-level vision, but debates concerning their interpretation have underestimated the role of multisensory mechanisms in such tests. Sensorimotor tasks engage multisensory processing in fundamentally different ways than perceptual tasks, and these differences can modulate dissociations in different ways based on task features. To test this idea, we compared perception and action using a well-understood size-contrast effect, the Uznadze illusion, and manipulated both unimodal and crossmodal stimulation as well as conditions that are known to favor or hinder multisensory integration. Results demonstrated that similar or dissociable effects on perception and action can be observed depending on factors that are known to affect multisensory processing. Specifically, such factors can cause a visual task to be affected by the illusion or remain fully unaffected, whereas a visuomotor task can be affected by the illusion, remain immune from the illusion, or, unexpectedly, even show a clear reverse effect. These findings provide a novel perspective on a long standing debate in behavioral cognitive neuroscience.

The alarming global rise in antibiotic resistance, driven by the widespread overuse of traditional antibiotics, has created an urgent demand for new antimicrobial solutions. This study presents zinc oxide (ZnO) nanorods as a potential nano-antibiotic agent. ZnO nanorods, with a 2:3 aspect ratio, were synthesized using an efficient one-step hydrothermal method at a low temperature of 100°C, reducing the synthesis time to just 5 hours. The synthesized ZnO nanorods' morphology, structure, and composition were characterized using scanning electron microscopy, x-ray diffraction, and energy dispersive x-ray spectroscopy. The potent antimicrobial activity of these nanorods against common bacterial strains such as Escherichia coli, Bacillus subtilis, and Vibrio parahaemolyticus was examined through optical density at 600 nm (OD600) measurements and inhibition zone analysis, demonstrating substantial inhibition of bacterial growth. In particular, at a concentration of 5 mg/mL, ZnO nanorods achieved a 96% reduction of B. subtilis bacteria in OD600 and an impressive 99.87% reduction in culturing assays within one day, showcasing bactericidal efficiency on par with tetracycline at 0.003 mg/mL. Furthermore, a predictive model of bacterial growth was developed and validated, providing insights into the time-dependent bactericidal efficiency of the synthesized nanorods. These results highlight the potential of ZnO-based composites as a promising solution to combat antibiotic resistance, paving the way for next-generation antimicrobial materials.