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Polyploidization disrupts drought response and epigenetic patterns in the desert wild potato species Solanum kurtzianum 沙漠野生马铃薯的多倍体化破坏了干旱响应和表观遗传模式。
IF 4.1 2区 生物学 Q2 BIOCHEMISTRY & MOLECULAR BIOLOGY Pub Date : 2026-02-02 DOI: 10.1016/j.plantsci.2026.113017
Damián N. Jerez , Carina V. González , Perla C. Kozub , Verónica N. Ibañez , Federico Berli , Ricardo W. Masuelli , Carlos F. Marfil
Polyploidy, the possession of more than two sets of chromosomes in a cell, is often linked to enhanced adaptability and stress resilience in plants, though it may introduce genomic instability and fitness costs. Here, we examined the interplay between ploidy level and epigenetic responses to drought stress in Solanum kurtzianum, a drought-tolerant wild relative of potato. We subjected diploid and newly oryzalin-induced autotetraploid lines to three different watering regimes and assessed their morphological, physiological, biochemical, and epigenetic responses. Diploids consistently outperformed autotetraploids in drought tolerance and tuber yield under moderate water stress, exhibiting lower stomatal conductance, yet maintaining comparable photochemical efficiency. Epigenetic analyses revealed significant interplay between the ploidy level and drought conditions on methylation patterns, with autotetraploids displaying higher methylation variability and greater genomic instability under severe drought. These findings are consistent with the predominance of diploid wild potatoes in arid regions and suggest that genomic instability caused by polyploidy may compromise drought resilience. The study emphasizes the adaptive potential of diploid wild potatoes in arid environments and the role of epigenetic mechanisms in stress responses. Our results have implications for potato breeding strategies, highlighting the potential of diploids for developing drought-resilient cultivars to cope with climate change challenges.
多倍体,即在一个细胞中拥有两组以上的染色体,通常与植物增强的适应性和抗逆性有关,尽管它可能会带来基因组的不稳定性和适应性成本。本研究研究了马铃薯的耐旱野生亲缘植物茄(Solanum kurtzianum)的倍性水平与表观遗传对干旱胁迫的反应之间的相互作用。我们将二倍体和新稻杂素诱导的同源四倍体系置于三种不同的浇水制度下,并评估了它们的形态、生理、生化和表观遗传反应。在中等水分胁迫下,二倍体在抗旱性和块茎产量方面始终优于同源四倍体,表现出较低的气孔导度,但保持相当的光化学效率。表观遗传分析显示,倍性水平和干旱条件对甲基化模式有显著的相互作用,同源四倍体在严重干旱条件下表现出更高的甲基化变异性和更大的基因组不稳定性。这些发现与二倍体野生马铃薯在干旱地区的优势一致,表明多倍体引起的基因组不稳定性可能会损害抗旱能力。本研究强调了二倍体野生马铃薯在干旱环境中的适应潜力以及表观遗传机制在逆境响应中的作用。我们的研究结果对马铃薯育种策略具有启示意义,突出了二倍体在开发抗旱品种以应对气候变化挑战方面的潜力。
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引用次数: 0
Construction of Kongyu 131 mutant library provides genetic resources for rice functional genomics and germplasm improvement 空育131突变文库的构建为水稻功能基因组学和种质改良提供遗传资源
IF 4.1 2区 生物学 Q2 BIOCHEMISTRY & MOLECULAR BIOLOGY Pub Date : 2026-01-31 DOI: 10.1016/j.plantsci.2026.113016
Tingting Xu , Qun Yang , Ran Li , Jianping Wang , Zhishan Luo , Daoyong Gong , Leihao Weng , Xiaoshan Huang , Debao Fu , Jing Li
Kongyu 131 (Oryza sativa ssp. japonica) is an elite rice cultivar widely planted in Heilongjiang Province, China. Here, we subjected Kongyu 131 to ethyl methanesulfonate (EMS)-induced mutagenesis to establish a genome-wide mutant library. On this basis, we generated 8770 M2 lines, and systematically screened 2215 lines with 42 distinct phenotypic variations across all developmental stages. Then, we employed a three-dimensional (3D) pooling strategy to combine 6144 M3 DNA samples into 224 multiplexed pools. By using the TILLING-seq (Targeting Induced Local Lesions in Genomes sequencing) approach, we identified 84 mutants across five key genes (GS3, Pi21, CSA, OsRR22, and OsaTRZ2), and validated their allelic variations. Phenotypic screening generated 18 lines with superior plant architecture, including a novel OsSPL14 allele identified via MutMap+ analysis. Whole-genome sequencing (WGS) of three M2 lines and TILLING-seq of mutants of the five key genes demonstrated a high mutation density (1 mutation per 121–288 kb). This 3D-pooled and genome-saturated EMS mutant library represents a robust resource for advancing functional genomic studies and precision breeding in rice.
空玉131 (Oryza sativa ssp)粳稻(japonica)是中国黑龙江省广泛种植的优良水稻品种。在此,我们对空玉131进行了甲基磺酸乙酯(EMS)诱变,建立了全基因组突变文库。在此基础上,我们获得了8770个M2株系,并系统筛选了2215个株系,在所有发育阶段有42个不同的表型变异。然后,我们采用三维(3D)池策略将6144 M3 DNA样本组合到224个多路池中。通过TILLING-seq (Targeting Induced Local lesion in Genomes sequencing)方法,我们鉴定了5个关键基因(GS3、Pi21、CSA、OsRR22和OsaTRZ2)中的84个突变体,并验证了它们的等位基因变异。表型筛选产生了18个具有优良植株结构的株系,其中包括一个通过MutMap+分析鉴定出的新的OsSPL14等位基因。3个M2系的全基因组测序和5个关键基因突变体的tillling -seq结果显示,突变密度较高(每121-288kb有1个突变)。这个3d池和基因组饱和的EMS突变文库为推进水稻功能基因组研究和精确育种提供了强大的资源。
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引用次数: 0
Transcriptomic analysis identifies VvMYB90 and VvNAC92 as key regulators mediating anthocyanin biosynthesis and fruit color differentiation in three grape cultivars 转录组学分析发现,VvMYB90和VvNAC92是3个葡萄品种花青素生物合成和果实颜色分化的关键调控因子。
IF 4.1 2区 生物学 Q2 BIOCHEMISTRY & MOLECULAR BIOLOGY Pub Date : 2026-01-30 DOI: 10.1016/j.plantsci.2026.113009
Han Lin , Kaili Mao , Shilong Chai, Jiangmin Lan, Hongbo Xiao, Zhixiong Guo, Tengfei Pan, Wenqin She
‘Shine Muscat’ (YG), ‘Gaoqiansui’ (GQS), and ‘Moldova’ (ME) are three grape cultivars with distinct phenotypic differences in fruits, serving as ideal materials for deciphering the mechanism of grape color differentiation. Currently, the specific mechanisms underlying the regulation of anthocyanin synthesis and color differentiation during grape fruit development require further investigation. To understand these, transcriptomic analysis was performed in this study to compare the different developmental stages of the three grape cultivars. Transcriptomic analysis displayed significant enrichment of differentially expressed genes (DEGs) in the phenylpropanoid and flavonoid biosynthesis pathways. These pathway-related genes exhibited a significant upregulation in expression during the veraison and maturation stages in the GQS and ME groups, with the most prominent upregulation observed in the ME group. Meanwhile, WGCNA and correlation network heatmap were employed to construct a TF-structural gene regulatory network associated with grape anthocyanin synthesis. VvMYB90 may mediate the regulation of gene-UFGT, whereas VvNAC92 may engage in the regulation of seven structural genes (gene-CHS, gene-GST4, gene-LOC100250360, gene-LOC100250579, gene-LOC100255217, gene-LOC100261962, and gene-UFGT). Collectively, these findings reveal the molecular basis of anthocyanin biosynthesis and color differentiation in grape fruits, providing meaningful insights into the accurate regulation of grape peel color.
“Shine Muscat”(YG)、“高干穗”(GQS)和“Moldova”(ME)是三个果实表型差异明显的葡萄品种,是研究葡萄颜色分化机制的理想材料。目前,葡萄果实发育过程中花青素合成和颜色分化调控的具体机制有待进一步研究。为了了解这些,本研究进行了转录组学分析,比较了三个葡萄品种的不同发育阶段。转录组学分析显示,在苯丙素和类黄酮生物合成途径中,差异表达基因(DEGs)显著富集。这些通路相关基因在GQS组和ME组的变异和成熟阶段均有显著的表达上调,其中ME组的表达上调最为显著。同时,利用WGCNA和相关网络热图构建葡萄花青素合成相关tf结构基因调控网络。VvMYB90可能介导- ufgt基因的调控,而VvNAC92可能参与7个结构基因(基因- chs、基因- gst4、基因- loc100250360、基因- loc100250579、基因- loc100255217、基因- loc100261962和基因- ufgt)的调控。总之,这些发现揭示了葡萄果实花青素生物合成和颜色分化的分子基础,为葡萄果皮颜色的准确调控提供了有意义的见解。
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引用次数: 0
Seed treatment technologies: Effects on physical, functional, and physiological seed quality 种子处理技术:对种子物理、功能和生理品质的影响。
IF 4.1 2区 生物学 Q2 BIOCHEMISTRY & MOLECULAR BIOLOGY Pub Date : 2026-01-30 DOI: 10.1016/j.plantsci.2026.113013
Venicius Urbano Vilela Reis , Everson Reis Carvalho , Imtiyaz Khanday
Seed treatment is a foundational technology in modern agriculture, designed to protect high-value seeds against initial pest and pathogen attacks, among other benefits, ensuring crop establishment. However, the application of complex chemical formulations, although protective, imposes stresses that can compromise seed quality if poorly performed. The objective of this review is to synthesize current knowledge on chemical seed treatment and critically analyze its impacts on the multiple attributes that define seed performance. The effects of seed treatment on physical quality are discussed, highlighting the risk of mechanical damage during processing, and on physiological quality, focusing on the mechanisms of phytotoxicity that can reduce vigor and germination, especially during storage, as well as all factors that can affect this relationship between treatment and physiological quality. Additionally, the emerging functional quality is addressed, analyzing how seed treatment affects plantability and environmental safety. A critical and often neglected balance exists between protective efficacy and seed integrity; therefore, studies for correct execution of seed treatment are essential for maintaining the physiological quality of seeds. Knowledge gaps, especially regarding the interaction between slurry mixture composition, storage, and initial lot quality, indicate the need for future research focused on safer formulations and optimized application technologies to maximize the benefits of seed treatment without compromising seed quality.
种子处理是现代农业的一项基础技术,其目的是保护高价值种子免受病虫害和病原体的侵袭,并确保作物成材。然而,应用复杂的化学配方,虽然保护,施加压力,可以损害种子质量,如果执行不当。这篇综述的目的是综合目前关于化学种子处理的知识,批判性地分析其对定义种子性能的多个属性的影响。讨论了种子处理对物理品质的影响,重点讨论了加工过程中机械损伤的风险;对生理品质的影响,重点讨论了植物毒性降低活力和发芽的机制,特别是在储存过程中,以及所有可能影响处理与生理品质之间关系的因素。此外,还讨论了新出现的功能质量,分析了种子处理如何影响可种植性和环境安全。主要结论是,在保护效果和保存种子完整性之间存在着一个关键的、经常被忽视的平衡;因此,正确执行的研究对于保持种子的生理品质至关重要。知识缺口,特别是关于浆料混合物组成、储存和初始批次质量之间的相互作用,表明未来需要研究更安全的配方和优化的应用技术,以最大限度地提高种子处理的效益,同时不影响种子质量。
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引用次数: 0
A BAHD acyltransferase of cotton affects plant drought tolerance and yield by regulating cuticle formation and cuticle permeability 棉花BAHD酰基转移酶通过调节角质层形成和角质层通透性影响植株抗旱性和产量。
IF 4.1 2区 生物学 Q2 BIOCHEMISTRY & MOLECULAR BIOLOGY Pub Date : 2026-01-30 DOI: 10.1016/j.plantsci.2026.113014
Xiaoxia Shangguan , Hongru Liu , Hongli Li , Huanyang Zhang , Jing Li , Zhiwen Chen
The cuticular layer in plants acts as a vital barrier against drought stress, with BAHD acyltransferase family members playing a key role in cuticle development. This study identified GhACY (GH_A11G0105), a BAHD family gene in cotton, located at the distal end of chromosome A11. Phylogenetic analysis placed GhACY in the second clade of subfamily Ⅰ, closely related to the DCR (defective in cuticular ridges; At5g23940) gene from Arabidopsis. Overexpression (OE) of GhACY in transgenic cotton enhanced drought tolerance and increased cotton yields compared to control lines. Conversely, RNA interference (RNAi)-mediated downregulation of GhACY compromised drought tolerance, with GhACY-RNAi transgenic lines exhibiting significantly reduced yield relative to wild-type plants. Chemical composition analysis revealed significant alteration in cutin and wax biosynthesis and deposition in transgenic cotton. In GhACY-RNAi plants, the content of wax and cutin monomer decreased by more than 35 %, with the predominant cutin compound, 18-hydroxy-9-octadecenoic acid (C18:9-ωHFA), reduced by 60 %. Specific wax compounds, including alkanes (especially nonacosane (C29), long-chain fatty acids, and hydroxylated fatty acids, were notably affected. In contrast, GhACY-OE plants exhibited a 35.4 % increase in total cutin monomer content. The levels of C18 monomers, particularly 18-hydroxy-9-octadecenoic acid (C18:9-ωHFA) and 10,18-trihydroxy-octadecanoic acid (C18:9,10,18-HFA), were significantly elevated compared to wild-type plants. These modifications reduced the permeability of the cotton leaf cuticle, thereby enhancing drought resistance and increasing cotton yield.
植物角质层是抵御干旱胁迫的重要屏障,BAHD酰基转移酶家族成员在角质层发育中起着关键作用。本研究鉴定出棉花BAHD家族基因GhACY (GH_A11G0105)位于A11染色体远端。系统发育分析将GhACY置于Ⅰ亚科的第二进化支,与拟南芥的DCR(表皮脊缺陷;At5g23940)基因密切相关。与对照棉花相比,转基因棉花中GhACY基因的过表达增强了棉花的耐旱性和产量。相反,RNA干扰(RNAi)介导的GhACY下调降低了抗旱性,与野生型植物相比,转基因GhACY-RNAi株系的产量显著降低。化学成分分析表明,转基因棉花的角质和蜡的生物合成和沉积发生了显著变化。在GhACY-RNAi植物中,蜡和角质层单体的含量下降了35%以上,其中主要的角质层化合物18-羟基-9-十八烯酸(C18:9 ω hfa)的含量下降了60%。特定的蜡化合物,包括烷烃(特别是壬烷烷(C29))、长链脂肪酸和羟基化脂肪酸,受到明显影响。相比之下,GhACY-OE植株的总角质单体含量增加了35.4%。C18单体,特别是18-羟基-9-十八烯酸(C18:9 ω hfa)和10,18-三羟基十八烯酸(C18:9,10,18- hfa)的含量显著高于野生型植物。这些修饰降低了棉花叶片角质层的渗透性,从而增强了抗旱性,提高了棉花产量。
{"title":"A BAHD acyltransferase of cotton affects plant drought tolerance and yield by regulating cuticle formation and cuticle permeability","authors":"Xiaoxia Shangguan ,&nbsp;Hongru Liu ,&nbsp;Hongli Li ,&nbsp;Huanyang Zhang ,&nbsp;Jing Li ,&nbsp;Zhiwen Chen","doi":"10.1016/j.plantsci.2026.113014","DOIUrl":"10.1016/j.plantsci.2026.113014","url":null,"abstract":"<div><div>The cuticular layer in plants acts as a vital barrier against drought stress, with BAHD acyltransferase family members playing a key role in cuticle development. This study identified <em>GhACY</em> (<em>GH_A11G0105</em>), a BAHD family gene in cotton, located at the distal end of chromosome A11. Phylogenetic analysis placed GhACY in the second clade of subfamily Ⅰ, closely related to the DCR (defective in cuticular ridges; <em>At5g23940</em>) gene from <em>Arabidopsis.</em> Overexpression (OE) of <em>GhACY</em> in transgenic cotton enhanced drought tolerance and increased cotton yields compared to control lines. Conversely, RNA interference (RNAi)-mediated downregulation of <em>GhACY</em> compromised drought tolerance, with <em>GhACY-RNAi</em> transgenic lines exhibiting significantly reduced yield relative to wild-type plants. Chemical composition analysis revealed significant alteration in cutin and wax biosynthesis and deposition in transgenic cotton. In <em>GhACY-RNAi</em> plants, the content of wax and cutin monomer decreased by more than 35 %, with the predominant cutin compound, 18-hydroxy-9-octadecenoic acid (C18:9-ωHFA), reduced by 60 %. Specific wax compounds, including alkanes (especially nonacosane (C29), long-chain fatty acids, and hydroxylated fatty acids, were notably affected. In contrast, <em>GhACY-OE</em> plants exhibited a 35.4 % increase in total cutin monomer content. The levels of C18 monomers, particularly 18-hydroxy-9-octadecenoic acid (C18:9-ωHFA) and 10,18-trihydroxy-octadecanoic acid (C18:9,10,18-HFA), were significantly elevated compared to wild-type plants. These modifications reduced the permeability of the cotton leaf cuticle, thereby enhancing drought resistance and increasing cotton yield.</div></div>","PeriodicalId":20273,"journal":{"name":"Plant Science","volume":"365 ","pages":"Article 113014"},"PeriodicalIF":4.1,"publicationDate":"2026-01-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146100505","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Seed priming with silicon nanoparticles and nitric oxide optimizes barley growth in zinc-deficient condition: a crucial role of optimum level of endogenous nitric oxide. 在缺锌条件下,用硅纳米颗粒和一氧化氮灌种可以优化大麦的生长:内源性一氧化氮的最佳水平起着至关重要的作用。
IF 4.1 2区 生物学 Q2 BIOCHEMISTRY & MOLECULAR BIOLOGY Pub Date : 2026-01-30 DOI: 10.1016/j.plantsci.2026.112998
Nidhi Kandhol, Sangeeta Pandey, Santosh Kumar, Shivesh Sharma, Samiksha Singh, Prasanta K Dash, Durgesh Kumar Tripathi

Zinc (Zn) deficiency significantly impacts plant growth and productivity in agriculture. Seed priming is a promising strategy to enhance plant tolerance to nutrient deficiencies. This study examines the effects of priming barley (Hordeum vulgare L.) seeds with silicon nanoparticles (SiNPs), nitric oxide (NO), and their combination on germination and growth under Zn-deficient conditions. Primed seedlings showed superior growth, and improved photosynthetic efficiency, antioxidant enzyme activities, the ascorbate-glutathione cycle function, nutrient-related gene expression, and sucrose metabolism compared to the un-primed seedlings. Among the priming methods, the combination of SiNPs and NO had the most significant positive effect on barley growth under Zn deficiency. Priming with SiNPs alone was more effective than external SiNPs application. Exogenous SiNPs added to SiNPs-primed seedlings further improved growth under Zn deficiency. Contrary to this, NO addition to NO-primed seedlings inhibited growth due to excessive endogenous NO accumulation. Co-application of SiNPs and NO to SiNPs+NO- primed seedlings led to severe growth retardation due to build-up of endogenous NO production. These findings highlight seed priming's potential, especially with SiNPs, to address nutrient deficiencies in agriculture and the complex interactions of endogenous NO in priming-mediated regulation of Zn deficiency in barley.

锌(Zn)缺乏严重影响农业植物的生长和生产力。种子启动是提高植物对营养缺乏的耐受性的一种很有前途的策略。在缺锌条件下,研究了纳米硅(SiNPs)和一氧化氮(NO)及其组合对大麦种子萌发和生长的影响。与未处理的幼苗相比,处理后的幼苗表现出更强的生长能力,光合效率、抗氧化酶活性、抗坏血酸-谷胱甘肽循环功能、营养相关基因表达和蔗糖代谢均有所提高。其中,SiNPs与NO组合对缺锌条件下大麦生长的正向影响最为显著。单独启动SiNPs比外用SiNPs更有效。在缺锌条件下,添加外源SiNPs进一步促进了幼苗的生长。与此相反,添加NO的幼苗由于内源NO积累过多而抑制生长。SiNPs和NO共同施用于SiNPs+NO引发的幼苗,由于内源性NO产量的增加,导致严重的生长迟缓。这些发现强调了种子启动的潜力,特别是与SiNPs一起,解决农业营养缺乏问题,以及内源NO在启动介导的大麦锌缺乏症调节中的复杂相互作用。
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引用次数: 0
BpbZIP61 negatively regulates drought resistance in birch by reducing ascorbic acid content BpbZIP61通过降低抗坏血酸含量负向调节桦树抗旱性。
IF 4.1 2区 生物学 Q2 BIOCHEMISTRY & MOLECULAR BIOLOGY Pub Date : 2026-01-30 DOI: 10.1016/j.plantsci.2026.113015
Wenfang Dong, Jiaojiao Wang, Xinyu Wang, Wenshuo Gao, Zhongyuan Liu, Caiqiu Gao
Drought is one of the major limiting factors affecting forest growth and survival. Basic leucine zipper (bZIP) transcription factors (TFs) play essential roles in plant responses to drought stress. Here, we identified that BpbZIP61, a drought-induced bZIP TF in birch (Betula platyphylla), functions as a potential negative regulator of drought tolerance. Overexpression of BpbZIP61 resulted in increased leaf water loss, elevated levels of reactive oxygen species (H₂O₂) and malondialdehyde (MDA), and decreased activities of Superoxide Dismutase (SOD) and Peroxidase (POD) activities. The overexpressing lines exhibited a significant reduction in ascorbic acid (AsA) content, a finding supported by transcriptomic evidence of downregulated expression in the ascorbate metabolism. We further demonstrated that BpbZIP61 functions as a transcriptional repressor by directly binding to ABRE cis-element in the promoter of BpGGLO6, a key gene in the AsA biosynthesis pathway, thereby suppressing its expression. The findings suggest that BpbZIP61 is a potential negative regulator for drought tolerance in birch, which operates by inhibiting AsA biosynthesis and regulating antioxidant enzyme activities, offering crucial insights for molecular breeding of drought-resistant trees.
干旱是影响森林生长和生存的主要限制因素之一。碱性亮氨酸拉链(bZIP)转录因子在植物对干旱胁迫的响应中起重要作用。本研究发现,白桦(Betula platyphylla)干旱诱导的bZIP转录因子BpbZIP61可能是干旱耐受性的负调控因子。BpbZIP61过表达导致叶片水分流失增加,活性氧(H₂O₂)和丙二醛(MDA)水平升高,超氧化物歧化酶(SOD)和过氧化物酶(POD)活性降低。过表达系抗坏血酸(AsA)含量显著降低,这一发现得到了抗坏血酸代谢下调表达的转录组学证据的支持。我们进一步证明,BpbZIP61通过直接结合AsA生物合成途径关键基因BpGGLO6启动子中的ABRE顺式元件,从而抑制其表达,从而发挥转录抑制因子的作用。研究结果表明,BpbZIP61是桦树耐旱性的潜在负调控因子,通过抑制AsA生物合成和调节抗氧化酶活性来发挥作用,为抗旱树木的分子育种提供了重要的见解。
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引用次数: 0
GmIFS interacts with GmNFR1α and plays a positive role in soybean legume-rhizobia symbiosis GmIFS与GmNFR1α相互作用,在大豆豆科植物与根瘤菌共生中发挥积极作用。
IF 4.1 2区 生物学 Q2 BIOCHEMISTRY & MOLECULAR BIOLOGY Pub Date : 2026-01-29 DOI: 10.1016/j.plantsci.2026.113011
Danxia Ke , Shibo Hou , Zhaoyuan Zhou
Soybean (Glycine max) serves as a vital source of plant protein and edible oil, while also functioning as a key soil-enriching crop. Symbiotic nitrogen fixation between soybean and rhizobia is crucial for sustainable green agriculture. Nod factor (NF) is a signaling molecule for the establishment of a symbiotic relationship between rhizobia and soybean. The soybean NF receptor GmNFR1α plays a pivotal role in nodulation; however, its signaling pathway remains incompletely characterized. In previous studies, using GmNFR1α as bait, we identified the isoflavone synthase GmIFS2 as an interactor through screening a soybean root/nodule yeast AD-cDNA library. Yeast two-hybrid, luciferase complementation imaging (LCI) in tobacco, and in vitro pull-down assays confirmed the interaction between GmIFS2 and the kinase domain of GmNFR1α (GmNFR1α-pk). Symbiotic phenotyping revealed that the ifs1/2 double mutant significantly inhibited the infection process of rhizobia, leading to a remarkable reduction in the number of soybean nodules and shoot/root dry weights. Integrated transcriptomic and metabolomic analyses of roots and nodules from ifs1/2 versus wild-type plants demonstrated substantial alterations in genes related to isoflavonoid synthesis, plant-pathogen interactions, and MAPK signaling pathways, alongside significant changes in key enzymes, transcription factors, and metabolites within isoflavonoid and nitrogen metabolism pathways. The study demonstrates that GmNFR1α can directly form a heteromeric complex with the soybean isoflavone synthase GmIFS2, positively regulating symbiotic nodulation between soybeans and rhizobia. The research findings further complement and elucidate the nodulation signaling pathway mediated by GmNFR1α, providing new molecular evidence for the symbiotic interaction mechanism between soybeans and rhizobia.
大豆(Glycine max)是植物蛋白和食用油的重要来源,同时也是重要的土壤肥力作物。大豆与根瘤菌的共生固氮对可持续绿色农业至关重要。结瘤因子(NF)是根瘤菌与大豆建立共生关系的信号分子。大豆NF受体GmNFR1α在结瘤过程中起关键作用;然而,其信号通路尚不完全清楚。在之前的研究中,我们以GmNFR1α为诱饵,通过筛选大豆根/根瘤酵母AD-cDNA文库,鉴定出异黄酮合成酶GmIFS2为相互作用物。酵母双杂交、烟草荧光素酶互补成像(LCI)和体外拉下实验证实了GmIFS2与GmNFR1α激酶结构域(GmNFR1α-pk)之间的相互作用。共生表型分析表明,ifs1/2双突变体显著抑制根瘤菌侵染过程,显著降低根瘤数和茎/根干重。综合转录组学和代谢组学分析表明,与野生型植物相比,ifs1/2的根和根瘤在异黄酮合成、植物与病原体相互作用和MAPK信号通路相关的基因上发生了实质性的变化,在异黄酮和氮代谢途径中,关键酶、转录因子和代谢物也发生了显著变化。研究表明,GmNFR1α可直接与大豆异黄酮合成酶GmIFS2形成异聚物复合物,正向调节大豆与根瘤菌的共生结瘤。本研究结果进一步补充和阐明了GmNFR1α介导的结瘤信号通路,为大豆与根瘤菌共生互作机制提供了新的分子证据。
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引用次数: 0
ABA and ABA signaling mediate Arabidopsis stomatal response to CO2 via H2O2 and NO production ABA和ABA信号通过产生H2O2和NO介导拟南芥气孔对CO2的响应
IF 4.1 2区 生物学 Q2 BIOCHEMISTRY & MOLECULAR BIOLOGY Pub Date : 2026-01-29 DOI: 10.1016/j.plantsci.2026.112993
Jinxia Li , Chenxi Zhang , Mingtao Mu , Xiaoyu Ma , Shumei Hei
The augmentation of atmospheric CO2 concentrations induces reduction in foliar stomatal aperture, thereby affecting photosynthetic capacity and transpiration- mediated cooling in plants. Elucidating the mechanism through which CO2 modulates stomatal dynamics is crucial for understanding plant adaptability to future fluctuations in CO2 levels. Although abscisic acid (ABA) is a pivotal phytohormone regulating stomatal movements, its role in CO2-induced stomatal closure remains unclear. Here, we demonstrate that elevated CO2 failed to induce stomatal closure in wild-type Arabidopsis treated with ABA biosynthesis inhibitor fluridon, as well as in the ABA-deficient mutant aba1, aba2 and aba3. Using β-glucuronidase (GUS) staining in pRD29B::GUS transgenic lines, we confirmed that the reporter gene controlled by ABA-responsive RD29B promoter in guard cells was strongly induced by elevated CO2. Additionally, transcripts of the ABA-responsive gene RAB18 and ABA-synthetic gene ABA2 were upregulated during CO2-induced stomatal closure in wild-type Arabidopsis. In contrast, fluridonand ABA receptor mutant pyr1pyl1pyl2pyl4 respectively inhibited CO2-induced activation of RD29B promoter-driven GUS marker and RAB18 expression. The expression of green fluorescent protein (GFP) reporter gene driven by ABA2 and RAB18 promoters in tobacco leaves was enhanced under CO2, and the fluorescence mainly distributed in non-guard cells. Furthermore, ABA, ABA receptor (PYR/RCARs) and OST1 kinase were identified as upstream cascade components of H2O2 and NO in stomatal response to CO2. Notably, GHR1 was implicated as an intermediate between H2O2 and NO in this pathway. Additionally, NADPH oxidase subunits AtRBOHD and AtRBOHF, and nitrate reductase isoenzyme NIA1, were responsible for H2O2 and NO production in guard cells under high CO2 conditions. Together, our findings propose that elevated CO2 triggers an increase in ABA and activates ABA signaling to close stomata via H2O2 and NO production, providing comprehensive insights into CO2 signaling in guard cells.
大气CO2浓度增加导致植物叶片气孔孔径减小,从而影响植物的光合能力和蒸腾降温作用。阐明二氧化碳调节气孔动力学的机制对于理解植物对未来二氧化碳水平波动的适应性至关重要。虽然脱落酸(ABA)是调节气孔运动的关键植物激素,但其在co2诱导的气孔关闭中的作用尚不清楚。在这里,我们证明了升高的CO2未能诱导野生型拟南芥在ABA生物合成抑制剂fluridon处理下的气孔关闭,以及ABA缺乏突变体aba1, aba2和aba3。通过对pRD29B::GUS转基因细胞系进行β-葡萄糖醛酸苷酶(GUS)染色,我们证实了保护细胞中由aba应答的RD29B启动子控制的报告基因受到高浓度CO2的强烈诱导。此外,野生型拟南芥在二氧化碳诱导的气孔关闭过程中,aba响应基因RAB18和aba合成基因ABA2的转录量上调。相比之下,氟啶酮和ABA受体突变体pyr1pyl1pyl2pyl4分别抑制二氧化碳诱导的RD29B启动子驱动的GUS标记激活和RAB18表达。CO2作用下,烟草叶片中由ABA2和RAB18启动子驱动的绿色荧光蛋白(GFP)报告基因表达增强,且荧光主要分布在非保护细胞中。此外,ABA、ABA受体(PYR/RCARs)和OST1激酶被确定为H2O2和NO在CO2响应中的上游级联组分。值得注意的是,GHR1在这一途径中作为H2O2和NO之间的中间体。此外,NADPH氧化酶亚基AtRBOHD和AtRBOHF以及硝酸还原酶同工酶NIA1在高CO2条件下负责保护细胞中H2O2和NO的产生。综上所述,我们的研究结果表明,升高的CO2会触发ABA的增加,并激活ABA信号,通过H2O2和NO的产生来关闭气孔,这为保护细胞中的CO2信号提供了全面的见解。
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引用次数: 0
Threshold decision-making in the Venus flytrap 捕蝇草的阈值决策。
IF 4.1 2区 生物学 Q2 BIOCHEMISTRY & MOLECULAR BIOLOGY Pub Date : 2026-01-29 DOI: 10.1016/j.plantsci.2026.113010
Tahir Rahman
The Venus flytrap (Dionaea muscipula) closes its trap only after two touches of its sensory hairs within approximately twenty seconds, reflecting a temporally gated threshold mechanism. Using a quantitative reinterpretation of published electrophysiological and mechanical measurements, this correspondence demonstrates that the bistable, memory-dependent closure of the Venus flytrap conforms to a multiplicative threshold framework (ARCH × Φ), in which four readiness domains, architecture (A), drive (D), context (C), and phase (Φ), jointly determine activation. The framework accounts for the two-trigger rule, refractory gating, and the all-or-none nature of trap closure, capturing the plant’s energy-efficient bistability.
捕蝇草(Dionaea muscipula)只有在大约20秒内两次接触它的感觉毛发后才会关闭捕蝇器,这反映了一种暂时封闭的阈值机制。通过对已发表的电生理和机械测量结果的定量重新解释,这种对应关系表明,捕蝇草的双稳态、依赖记忆的闭合符合乘法阈值框架(ARCH × Φ),其中四个准备域,结构(a)、驱动(D)、环境(C)和相位(Φ)共同决定激活。该框架考虑了双触发规则、耐火门控和全或无陷阱关闭的性质,捕捉了工厂的节能双稳定性。
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引用次数: 0
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Plant Science
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