Plant Science最新文献_第4页

The TIFY transcription factor ZmJAZ13 enhances plant tolerance to drought and salt stress by interacting with ZmbHLH161 and ZmA0A1D6GLB9 TIFY转录因子ZmJAZ13通过与ZmbHLH161和ZmA0A1D6GLB9相互作用增强植物对干旱和盐胁迫的耐受性。

IF 4.2 2区生物学 Q2 BIOCHEMISTRY & MOLECULAR BIOLOGY

Plant Science

Pub Date : 2025-01-13 DOI: 10.1016/j.plantsci.2025.112388

Shipeng Zhang , Dengyu Zheng , Yuqi Gao , Meng She , Zhongyi Wu , Yuncai Lu , Zhongbao Zhang

The JAZ protein family, serving as a key negative regulator in the jasmonic acid signaling pathway, interacts with transcription factors to play an essential role in plant growth, development, and stress responses. However, minimal research has focused on the role of JAZ transcription factors in regulating the growth, development, and stress responses of maize. In this study, we cloned the JAZ gene ZmJAZ13 from maize (Zea mays L.) and conducted a preliminary analysis of its biological function. ZmJAZ13 was highly expressed in maize immature embryos and was induced by abiotic stress and plant hormone treatments. Y2H and BiFC assays revealed interactions between ZmJAZ13 and ZmbHLH161, as well as ZmA0A1D6GLB9. Heterologous expression of ZmJAZ13 in Arabidopsis significantly enhanced plant tolerance to drought and salt stress, increased chlorophyll content, decreased malondialdehyde content, and enhanced peroxidase activity. Under abiotic stress, heterologous expression of ZmJAZ13 in Arabidopsis upregulated the expression levels of stress-related genes (RD22, RD29-A). Together, these results suggested that ZmJAZ13 may respond to abiotic stress, providing a foundation for further investigation into the mechanism of action of ZmJAZ13 in maize.

JAZ蛋白家族是茉莉酸信号通路的关键负调控蛋白，与转录因子相互作用，在植物生长发育和逆境响应中发挥重要作用。然而，很少有研究集中在JAZ转录因子在调节玉米生长、发育和胁迫反应中的作用。本研究从玉米（Zea mays L.）中克隆了JAZ基因ZmJAZ13，并对其生物学功能进行了初步分析。ZmJAZ13在玉米未成熟胚中高表达，受非生物胁迫和植物激素诱导。Y2H和BiFC检测显示ZmJAZ13和ZmbHLH161以及ZmA0A1D6GLB9之间存在相互作用。ZmJAZ13在拟南芥中的异源表达显著增强了植物对干旱和盐胁迫的耐受性，提高了叶绿素含量，降低了丙二醛含量，增强了过氧化物酶活性。在非生物胁迫下，异源表达ZmJAZ13可上调胁迫相关基因（RD22, RD29-A）的表达水平。以上结果提示，ZmJAZ13可能对非生物胁迫有响应，为进一步研究ZmJAZ13在玉米中的作用机制奠定了基础。

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引用次数: 0

GhWRKY207 improves drought tolerance through promoting the expression of GhCSD3 and GhFSD2 in Gossypium hirsutum GhWRKY207通过促进棉花中GhCSD3和GhFSD2的表达来提高抗旱性。

IF 4.2 2区生物学 Q2 BIOCHEMISTRY & MOLECULAR BIOLOGY

Plant Science

Pub Date : 2025-01-12 DOI: 10.1016/j.plantsci.2025.112392

Gaofeng Zhang , Weichao Li , Tong Han , Tianyi Huang , Lirong Sun , Fushun Hao

Tryptophan-arginine-lysine-tyrosine (WRKY) transcription factors are essential regulators of drought tolerance in multiple plants. However, whether and how GhWRKY207 modulates cotton response to drought stress is unclear. In this study, we determined that GhWRKY207 expression was high in leaves and induced by drought stress. The gene encoded a nuclear protein that had transcriptional activation activity. Silencing GhWRKY207 by virus-induced gene silencing (VIGS) caused significant reduction in drought tolerance of cotton plants. Consistently, overexpression of GhWRKY207 in Arabidopsis thaliana wild type (WT) plants clearly enhanced their drought tolerance. Moreover, GhWRKY207 VIGS plants had notably increased malondialdehyde (MDA) contents, electrolyte leakage percentages and O₂^·− accumulation rates whereas GhWRKY207 overexpression lines showed markedly decreased levels of the three parameters compared to their corresponding controls under water deficit conditions. Additionally, GhWRKY207 enhanced superoxide dismutase (SOD) activity by directly activating the expression of GhCu/Zn-SOD3 (GhCSD3) and GhFe-SOD2 (GhFSD2) genes. Silencing GhCSD3 or GhFSD2 also markedly reduced drought tolerance of cotton plants. Taken together, these results suggest that GhWRKY207 positively regulates drought tolerance by inducing the expression of GhCSD3 and GhFSD2 in Gossypium hirsutum.

色氨酸-精氨酸-赖氨酸-酪氨酸（WRKY）转录因子是多种植物抗旱性的重要调控因子。然而，GhWRKY207是否以及如何调节棉花对干旱胁迫的反应尚不清楚。在本研究中，我们确定了GhWRKY207在叶片中高表达，并受到干旱胁迫的诱导。它编码了一种具有转录激活活性的核蛋白。通过病毒诱导基因沉默（VIGS）对GhWRKY207进行沉默处理，棉花耐旱性显著降低。与此同时，过表达GhWRKY207在拟南芥野生型（WT）植物中明显增强了其抗旱性。此外，在水分亏缺条件下，GhWRKY207 VIGS植株丙二醛（MDA）含量、电解质泄漏百分比和O2·-积累速率显著增加，而GhWRKY207过表达系的这三个参数水平均显著低于对照。此外，GhWRKY207通过直接激活GhCu/Zn-SOD3 （GhCSD3）和GhFe-SOD2 （GhFSD2）基因的表达，增强了超氧化物歧化酶（SOD）活性。沉默GhCSD3或GhFSD2也显著降低了棉花植株的抗旱性。综上所述，GhWRKY207通过诱导棉花中GhCSD3和GhFSD2的表达，正向调节棉花的耐旱性。

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引用次数: 0

Effects of reactive oxygen species on fruit ripening and postharvest fruit quality 活性氧对果实成熟和采后果实品质的影响。

IF 4.2 2区生物学 Q2 BIOCHEMISTRY & MOLECULAR BIOLOGY

Plant Science

Pub Date : 2025-01-12 DOI: 10.1016/j.plantsci.2025.112391

Wenying Wei, Zesheng Liu, Xuejuan Pan, Tingyue Yang, Caiting An, Yuanhui Wang, Long Li, Weibiao Liao, Chunlei Wang

Reactive oxygen species (ROS) serve as important signaling molecule, involved in numerous biological processes, particularly in the physiological changes associated with fruit ripening and postharvest handing. This review explores ROS key role in plant fruit ripening and postharvest quality. The mechanism of ROS production and degradation in maintaining ROS homeostasis are analyzed in detail. Fruit ripening is a complex and highly coordinated process involving physiological and biochemical changes. Studies have observed that the content of ROS, mainly hydrogen peroxide (H₂O₂), dynamically changes in various types of fruits during ripening. Furthermore, ROS have significant effects on fruit softening, color change, and other ripening processes. In addition, in the postharvest stage, the abnormal accumulation of ROS isclosely related to the decline in fruit quality and the occurrence of decay browning, which seriously affects the market value and shelf life of fruit. Overall, this review demonstrates the crucial role of ROS in regulating the ripening process and postharvest quality of fruit.

活性氧（Reactive oxygen species， ROS）是一种重要的信号分子，参与许多生物过程，特别是与果实成熟和采后处理相关的生理变化。本文综述了活性氧在植物果实成熟和采后品质中的重要作用。详细分析了活性氧产生和降解维持活性氧稳态的机制。果实成熟是一个涉及生理生化变化的复杂而高度协调的过程。研究发现，在不同类型水果的成熟过程中，活性氧（ROS），主要是过氧化氢（H2O2）的含量是动态变化的。此外，活性氧对果实的软化、颜色变化等成熟过程有显著影响。此外，在采后阶段，ROS的异常积累与果实品质下降和腐烂褐变的发生密切相关，严重影响果实的市场价值和保质期。综上所述，本文综述了活性氧在调节果实成熟过程和采后品质中的重要作用。

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引用次数: 0

Rice THIN CULM 4 (TC4) modulates culm strength by regulating morphology, structure, and development 水稻 THIN CULM 4 (TC4) 通过调节形态、结构和发育来调节茎秆强度。

IF 4.2 2区生物学 Q2 BIOCHEMISTRY & MOLECULAR BIOLOGY

Plant Science

Pub Date : 2025-01-10 DOI: 10.1016/j.plantsci.2024.112375

Ran Zhou , Chenbo Huang , Xiaoxia Wen , Zhihao Sun , Wei Dong , Yuyu Chen , Nuan Huang , Han Zhang , Haihan Su , Yanhui Li , Zequn Peng , Yingxin Zhang , Liyong Cao , Shihua Cheng , Xiaodeng Zhan , Lianping Sun , Daibo Chen

Culm strength is crucial for rice growth, nutrition transportation, and structural resilience, which are essential for lodging resistance and stable production. In this study, we identified a rice thin culm mutant tc4, characterized by thinner culms and thicker cavity walls, resulting in weakened culm mechanical strength. Using map-based cloning, the candidate gene was isolated, and complementation and CRISPR/Cas9 experiments confirmed that a single nucleotide substitution in TC4 is responsible for the thin and brittle culm phenotype. TC4, a homolog of the FLORICAULA/LEAFY gene, localizes to the nucleus and cytoplasm. Further research revealed that TC4 regulates culm development by influencing plant hormones and sugar transport. This research not only advances our understanding of rice culm regulation, but also provides valuable insights for breeding lodging-resistant rice varieties.

茎秆强度对水稻生长、营养转运和结构抗逆性至关重要，对水稻抗倒伏和稳定生产至关重要。在这项研究中，我们鉴定了一个水稻薄秆突变体tc4，其特征是茎变薄，空腔壁变厚，导致茎的机械强度减弱。利用图谱克隆技术分离出候选基因，并进行了互补和CRISPR/Cas9实验，证实TC4中的单核苷酸替换是导致茎薄脆表型的原因。TC4是FLORICAULA/LEAFY基因的同源物，定位于细胞核和细胞质中。进一步研究发现，TC4通过影响植物激素和糖的转运来调控茎的发育。该研究不仅提高了我们对水稻茎秆调控的认识，而且为水稻抗倒伏品种的选育提供了有价值的见解。

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引用次数: 0

3,4-dimethylpyrazole phosphate (DMPP) may negate the expected stimulation of elevated atmospheric CO2 and warming on fertilizer-N loss 3,4-二甲基吡唑磷酸（DMPP）可能抵消了预期的大气CO2升高和变暖对肥料n损失的刺激。

IF 4.2 2区生物学 Q2 BIOCHEMISTRY & MOLECULAR BIOLOGY

Plant Science

Pub Date : 2025-01-09 DOI: 10.1016/j.plantsci.2025.112386

Wenjie Zhang , Lin Zhao , Ting Zhang , Mengyun Shi , Dianjun Lu , Shuai Wang , Jia Zhang , Wei Jiang , Meng Wei

People have accepted the clear fact that elevated CO₂ (eCO₂) and climate warming are happening, but sustainable agricultural systems are still struggling to adapt. 3,4-dimethyl-1H-pyrazol phosphate (DMPP) is currently recognized as a highly effective strategy for reducing nitrogen (N) loss and related environmental impacts. There is still uncertainty, however, whether DMPP could contribute to building climate-resilient ecosystems in a future climate scenario with co-elevated CO₂ and temperature. Thus, this study evaluated the responses of plant N derived from soil or fertilizer and strawberry growth to the tested climate conditions. Plants were supplied with or without DMPP, grown in controlled climate chambers under ambient CO₂ and temperature (aCT; 400 ppm + 25℃), and co-elevated CO₂ and temperature (eCT; 800 ppm + 27℃). The results showed that DMPP increased plant N accumulation by 9 % and 19 % under aCT and eCT conditions, respectively, compared to N treatment without DMPP. We also found a similar trend in total C content in the plants. Compared with aCT, DMPP demonstrated higher efficiency in improving N use efficiency (NUE, 51 % vs. 36 %) and reducing N loss (21 % vs. 29 %) under eCT, which could ensure higher N demand of plant, making fertilizer-N, rather than soil-N, a primary contributor to the N accumulation increment. Moreover, in terms of combating climate challenge, the combination with DMPP further strengthened the beneficial influence of eCT on the N accumulation and biomass in strawberry but reduced fertilizer-N loss. In summary, DMPP exhibits better performance under eCT, which may alleviate the potential adverse effects of co-elevated CO₂ and temperature on ecosystem by reducing fertilizer-N loss and soil-N mineralization more efficiently, providing a promising approach to optimizing sustainable agricultural management under future climate change.

人们已经接受了二氧化碳（eCO2）升高和气候变暖正在发生的明确事实，但可持续农业系统仍在努力适应。3,4-二甲基- 1h -吡唑磷酸（DMPP）是目前公认的减少氮(N)损失和相关环境影响的高效策略。然而，在二氧化碳和温度共同升高的未来气候情景中，DMPP是否有助于建立具有气候适应性的生态系统仍存在不确定性。因此，本研究评估了来自土壤或肥料的植物氮和草莓生长对试验气候条件的响应。提供或不提供DMPP的植物，在环境CO2和温度(aCT；400ppm + 25℃)，并共同升高CO2和温度(eCT；800ppm + 27℃)。结果表明，与不施DMPP的氮素处理相比，aCT和eCT处理下DMPP分别使植株氮积累量增加9%和19%。我们还发现植物中总碳含量也有类似的趋势。与aCT相比，DMPP在提高氮素利用效率（NUE分别为51%和36%）和减少氮素损失（21%和29%）方面表现出更高的效率，保证了植物对氮的更高需求，使肥料氮而非土壤氮成为氮素积累增量的主要贡献者。此外，在应对气候挑战方面，与DMPP联合施用进一步增强了电刺激对草莓氮素积累和生物量的有益影响，但减少了氮肥损失。综上所述，DMPP在高温胁迫下表现出更好的性能，可能通过更有效地减少氮肥损失和土壤氮矿化，缓解CO2和温度共同升高对生态系统的潜在不利影响，为未来气候变化下优化农业可持续管理提供了一条有希望的途径。

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引用次数: 0

WD40 proteins PaTTG1 interact with both bHLH and MYB to regulate trichome formation and anthocyanin biosynthesis in Platanus acerifolia WD40蛋白PaTTG1与bHLH和MYB相互作用，调节毛囊形成和花青素的生物合成。

IF 4.2 2区生物学 Q2 BIOCHEMISTRY & MOLECULAR BIOLOGY

Plant Science

Pub Date : 2025-01-09 DOI: 10.1016/j.plantsci.2025.112385

Qi Mengxuan, Tian Xinyue, Chen Yuqing, Lu Yongkang, Zhang Yanping

Trichome development and anthocyanin accumulation are regulated by a complex regulatory network, the MBW complexe consists of MYB, bHLH, and WD40 transcription factors. In this study, two sequences, named PaTTG1.1, and PaTTG1.2, were cloned and functionally characterized from Platanus acerifolia. Quantitative real-time PCR results showed that PaTTG1 genes were expressed in the trichomes and red leaves. Overexpression of PaTTG1.1 and PaTTG1.2 genes in Arabidopsis ttg1 mutants restored the phenotypes of ttg1 mutants that were glabrous and lacked purple anthocyanins in hypocotyls and seeds. In transgenic plants, the expression levels of the trichome regulation-related genes AtCPC, AtTRY, AtETC1, AtMYB23, and AtGL2, as well as early and late biosynthetic genes related to anthocyanin biosynthesis, were significantly upregulated. The results of the yeast two-hybrid showed that PaTTG1.1 and PaTTG1.2 proteins could physically interact with both bHLH and R2R3-MYB transcription factors from Arabidopsis and P. Acerifolia. Taken together, the results presented in this study suggest that the two PaTTG1 genes share similar functions in the regulation of trichomes and anthocyanins. However, there may be some differences in their regulatory mechanisms.

毛状体发育和花青素积累受一个复杂的调控网络调控，MBW复合物由MYB、bHLH和WD40转录因子组成。本研究克隆了两个序列，命名为PaTTG1.1和PaTTG1.2，并对其进行了功能鉴定。实时荧光定量PCR结果显示，PaTTG1基因在毛状体和红叶中均有表达。在拟南芥ttg1突变体中过表达PaTTG1.1和PaTTG1.2基因，恢复了ttg1突变体下胚轴和种子中无毛且缺乏紫色花青素的表型。在转基因植株中，毛状体调控相关基因AtCPC、AtTRY、AtETC1、AtMYB23、AtGL2以及与花青素生物合成相关的早期和晚期生物合成基因的表达水平均显著上调。酵母双杂交结果表明，PaTTG1.1和PaTTG1.2蛋白可与拟南芥和荆芥的bHLH和R2R3-MYB转录因子发生物理相互作用。综上所述，本研究的结果表明，在不同物种中，两个PaTTG1基因在调节毛状体和花青素方面具有相似的功能。然而，它们的监管机制可能存在一些差异。

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引用次数: 0

Genome-wide association study of rice (Oryza sativa L.) inflorescence architecture 水稻花序结构的全基因组关联研究。

IF 4.2 2区生物学 Q2 BIOCHEMISTRY & MOLECULAR BIOLOGY

Plant Science

Pub Date : 2025-01-09 DOI: 10.1016/j.plantsci.2024.112382

Masoumeh Kordi , Naser Farrokhi , Asadollah Ahmadikhah , Pär K. Ingvarsson , Abbas Saidi , Mehdi Jahanfar

Rice yield strongly depends on panicle size and architecture but the genetics underlying these traits and their coordination with environmental cues through various signaling pathways have remained elusive. A genome-wide association study (GWAS) was performed to pinpoint the underlying genetic determinants for rice panicle architecture by analyzing 20 panicle-related traits using a data set consisting of 44,100 SNPs. We defined QTL windows around significant SNPs by the rate of LD decay for each chromosome and used these windows to identify putative candidate genes associated with the trait. Using a publicly available RNA-seq data set we performed analyses to identify the differentially expressed genes between stem and panicle with putative functions in panicle architecture. In total, 52 significant SNPs were identified, corresponding to 41 unique QTLs across the 12 rice chromosomes, with the most signals appearing on chromosome 1 (nine associated SNPs), and seven significant SNPs for each of chromosomes 8 and 12. Some novel genes such as Ankyrin, Duf, Kinesin and Brassinosteroid insensitive were found to be associated with panicle size. A haplotype analysis showed that genetic variation in haplotypes qMIL2 and qNSBBH21 were related to two traits, MIL, the greatest distance between two nodes on the rachis, and NSBBH, the number of primary branches in the bottom half of a panicle, respectively. Analysis of epistatic interactions revealed a marker affecting clustered traits. Several QTLs were identified on different chromosomes for the first time which may explain the phenotypic diversity of rice panicle architecture we observe in our collection of accessions. The identified candidate genes and haplotypes could be used in marker-assisted selection to improve rice yield through gene pyramiding.

水稻产量在很大程度上取决于穗大小和结构，但这些性状的遗传基础及其通过各种信号通路与环境信号的协调仍然是难以捉摸的。利用包含44100个snp的数据集分析了20个水稻穗相关性状，开展了一项全基因组关联研究（GWAS），以确定水稻穗结构的潜在遗传决定因素。我们根据每条染色体的LD衰减率定义了显著snp周围的QTL窗口，并使用这些窗口识别与该性状相关的假定候选基因。利用公开可用的RNA-seq数据集，我们进行了分析，以确定茎和穗之间的差异表达基因，这些基因可能在穗结构中起作用。总共鉴定出52个显著snp，对应12条水稻染色体中的41个独特qtl，其中1号染色体上出现的信号最多（9个相关snp）， 8号和12号染色体各有7个显著snp。一些新的基因如锚蛋白（Ankyrin）、Duf、肌动蛋白（Kinesin）和油菜素内酯不敏感基因（brassino类固醇不敏感基因）与穗大小有关。单倍型分析表明，qMIL2和qNSBBH21两种单倍型的遗传变异分别与茎轴最大节距MIL和穗下半部分初级分枝数NSBBH有关。上位互作分析揭示了一个影响聚类性状的标记。在不同的染色体上首次鉴定出几个qtl，这可能解释了我们在材料中观察到的水稻穗型结构的表型多样性。所鉴定的候选基因和单倍型可用于标记辅助选择，通过基因金字塔技术提高水稻产量。

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引用次数: 0

Synthetic elicitors-induced defense in crops against herbivory: A review 合成诱导剂诱导的作物草食防御研究进展。

IF 4.2 2区生物学 Q2 BIOCHEMISTRY & MOLECULAR BIOLOGY

Plant Science

Pub Date : 2025-01-08 DOI: 10.1016/j.plantsci.2025.112387

Malawanthkar Rani, Ramasamy Kanagaraj Murali-Baskaran

Synthetic elicitors are non-toxic chemicals and safe for the environment when applied to plants in a variety of ways. They have been shown to interact with defense mechanisms of plants and cause the production of a wide range of valuable secondary metabolites, both volatile and non-volatile. Plants primed with chemical elicitors are indirectly induced to increase their resistance to herbivore attacks in addition to imparting tolerance or resistance to nearby plants against biotic stresses. The market is stocked with jasmonic acid, salicylic acid, and their derivatives/analogues, which have been shown to either repel or attract herbivores. While phytotoxicity has only been documented in a small number of cases, a significant increase in yield has been reported in a wide range of crops. This review includes a detailed summary of various field and laboratory experiments elucidating the mechanism of action and efficacies of exogenous application and seed priming of synthetic phytohormones on plant growth, development, and yield of different crops.

当以各种方式应用于植物时，合成激发子是无毒的化学品，对环境是安全的。它们已被证明与植物的防御机制相互作用，并导致一系列有价值的次生代谢物的产生，包括挥发性和非挥发性。通过化学激发子间接诱导植物增加对食草动物攻击的抵抗力，并使附近植物对生物胁迫具有耐受性或抗性。市场上充斥着茉莉酸、水杨酸和它们的衍生物/类似物，它们被证明要么排斥要么吸引食草动物。虽然植物毒性仅在少数情况下被记录在案，但据报道，许多作物的产量都有显著增加。本文详细综述了各种田间和室内实验，阐明了外源施用和种子启动对不同作物生长、发育和产量的作用机制和效果。

引用次数: 0

Retraction notice to “Scorpion peptide LqhIT2 activates phenylpropanoid pathways via jasmonate to increase rice resistance to rice leafrollers” [Plant Sci. 230 (2015) 1–11]

IF 4.2 2区生物学 Q2 BIOCHEMISTRY & MOLECULAR BIOLOGY

Plant Science

Pub Date : 2025-01-07 DOI: 10.1016/j.plantsci.2024.112372

Xiuzi Tianpei , Dong Li , Ping Qiu , Jie Luo , Yingguo Zhu , Shaoqing Li

引用次数: 0

Overexpression of SikPsaF can increase the biomass of Broussonetia papyrifera by improving its photosynthetic efficiency and cold tolerance SikPsaF过表达可以通过提高纸莎草的光合效率和耐寒性来增加其生物量。

IF 4.2 2区生物学 Q2 BIOCHEMISTRY & MOLECULAR BIOLOGY

Plant Science

Pub Date : 2025-01-03 DOI: 10.1016/j.plantsci.2024.112380

Mei Wang , Xiangxue Yu , Jingyi Zhao , Zhijia Tian , Bo Chen , Qian Li , Dingguo Zhang , Fanfan Zhang , Li Zhang , Xinyong Guo

Photosynthesis is essential for the accumulation of organic compounds in plant leaves. Study of photosynthesis in the leaves of Broussonetia papyrifera is crucial for enhancing its biomass production, growth, and development. Here, we cloned the SikPsaF gene associated with photosynthesis from Saussurea involucrata and constructed a vector that was introduced into B. papyrifera to generate a transgenic strain. We then assessed various photosynthesis-related parameters in the transgenic plants and examined the function of this gene and its expression patterns under cold stress. The results showed that SikPsaF was localized to chloroplasts. Its expression was induced by light, and its expression was higher in the leaves than in other tissues. Furthermore, SikPsaF expression increased significantly under cold stress. The biomass of transgenic lines was greater than that of wild-type plants. Overexpression of this gene led to increases in the chlorophyll content and photosynthetic indices, which mitigated cell membrane damage and reduced reactive oxygen species (ROS) accumulation. SikPsaF overexpression also helped maintain high antioxidant enzyme activity and a high content of osmoregulatory substances during stress; the increased enzyme activities were due to up-regulated gene expression. Overexpression of SikPsaF has a major effect on growth and development by enhancing photosynthetic efficiency, improving yield, conferring cold resistance, and reducing damage to the cell membrane and ROS accumulation at low temperatures. In summary, our findings indicate that these transgenic plants have enhanced photosynthetic efficiency and resilience against biotic stresses.

光合作用对植物叶片中有机化合物的积累至关重要。研究纸莎草（Broussonetia papyrifera）叶片的光合作用对提高其生物量生产、生长和发育至关重要。在此，我们克隆了 Saussurea involucrata 中与光合作用相关的 SikPsaF 基因，并构建了一个载体，将其导入纸莎草叶中，生成转基因株系。然后，我们评估了转基因植株的各种光合作用相关参数，并研究了该基因的功能及其在冷胁迫下的表达模式。结果表明，SikPsaF定位于叶绿体。其表达受光照诱导，叶片中的表达量高于其他组织。此外，SikPsaF的表达在冷胁迫下显著增加。转基因品系的生物量高于野生型植株。该基因的过表达导致叶绿素含量和光合指数增加，从而减轻了细胞膜损伤，减少了活性氧（ROS）积累。SikPsaF 的过表达还有助于在胁迫期间维持高抗氧化酶活性和高渗透调节物质含量；酶活性的提高是由于基因表达的上调。SikPsaF 的过表达对生长发育有重要影响，它能提高光合效率，提高产量，赋予抗寒性，减少低温条件下细胞膜的损伤和 ROS 的积累。总之，我们的研究结果表明，这些转基因植物具有更高的光合效率和抵御生物胁迫的能力。

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引用次数: 0