Plant Science最新文献

{"title":"ATP-binding cassette G23 is required for Arabidopsis seed coat suberization","authors":"Ryeo Jin Kim,&nbsp;Yuyang Zhang,&nbsp;Mi Chung Suh","doi":"10.1016/j.plantsci.2024.112361","DOIUrl":"10.1016/j.plantsci.2024.112361","url":null,"abstract":"<div><div>Suberin is an extracellular hydrophobic polymer deposited in seed coats that acts as a barrier to regulate the movement of ions, water, and gases, and protects seeds against pathogens. However, the molecular mechanisms underlying suberin deposition in the seed coat remain unknown. In this study, the <em>in planta</em> role of ATP-binding cassette G23 (ABCG23) was investigated in the Arabidopsis seed coat. <em>ABCG23</em> transcripts were predominantly expressed in the outer integument1 (oi1) of seed coats and the endodermal cells of roots. The fluorescence of the <em>eYFP:ABCG23</em> construct was observed in the plasma membranes of the tobacco epidermis, seed coat oi1, and root endodermal cells. Seed coats of <em>abcg23–1</em> and <em>abcg23–2</em> mutants exhibited reduced autofluorescence under UV light and increased permeability to tetrazolium salts. Total suberin loads and major suberin components, C24 ω-hydroxy fatty acids and 1, ω-dicarboxylic acids were significantly decreased in the mutant seed coats. The ratio of seed germination and seedling establishment of <em>abcg23–1</em> and <em>abcg23–2</em> was significantly reduced compared to the WT under salt and osmotic stress conditions. The bimolecular fluorescence complementation assay showed homodimeric interactions of ABCG-2, −6, −20, and −23 and heterodimeric interactions between ABCG23 and ABCG-2, −6, −11, or −20. Our findings indicate that ABCG23 contributes to the transport of suberin monomers in the Arabidopsis seed coats.</div></div>","PeriodicalId":20273,"journal":{"name":"Plant Science","volume":"352 ","pages":"Article 112361"},"PeriodicalIF":4.2,"publicationDate":"2024-12-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142865348","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Enhancing starch levels, granule size and phosphate content in Chlamydomonas reinhardtii through overexpression of ChlreSEX4","authors":"Florencia Torresi ,&nbsp;Julieta B. Carrillo ,&nbsp;Diego F. Gomez-Casati ,&nbsp;Maria V. Busi ,&nbsp;Mariana Martín","doi":"10.1016/j.plantsci.2024.112360","DOIUrl":"10.1016/j.plantsci.2024.112360","url":null,"abstract":"<div><div><em>Chlamydomonas reinhardtii</em> is a green alga that has been widely used as a model organism for studying various cellular processes, including starch metabolism. In this alga, starch undergoes continuous phosphorylation during its synthesis and degradation. We recently identified and characterized <em>Chlre</em>SEX4 (starch excess 4), a glucan phosphatase from <em>C. reinhardtii,</em> orthologous to <em>Arabidopsis thaliana</em> SEX4, which is able to bind and dephosphorylate amylopectin <em>in vitro</em>. To explore the possibility of manipulating starch phosphorylation levels in <em>C. reinhardtii</em>, we overexpressed the <em>Chlre</em>SEX4 gene in <em>Chlamydomonas</em> and characterized the resulting lines. Results showed a high phosphatase activity in the overexpressing lines, accompanied by an increase in starch content, greater granule size and higher levels of granule-bound phosphate, without changes in triglyceride content. This work allowed us not only to discover a new method to enhance starch accumulation without affecting the lipid content of the alga, but also to obtain a more phosphorylated starch, which would have diverse applications in biotechnology.</div></div>","PeriodicalId":20273,"journal":{"name":"Plant Science","volume":"352 ","pages":"Article 112360"},"PeriodicalIF":4.2,"publicationDate":"2024-12-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142838639","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effect analysis of S5-interacting genes on rice hybrid sterility using nontransgenic gamete killer","authors":"Jie Li ,&nbsp;Fu Huang ,&nbsp;Yingxia Jiang,&nbsp;Jianglei Rao,&nbsp;Yourong Fan,&nbsp;Jiangyi Yang","doi":"10.1016/j.plantsci.2024.112357","DOIUrl":"10.1016/j.plantsci.2024.112357","url":null,"abstract":"<div><div>While hybrids between <em>japonica</em> and <em>indica</em> rice exhibit strong heterosis, they often suffer from hybrid sterility (HS). Hybrid fertility of the embryo sac is predominantly regulated by a three-gene system (comprising closely linked <em>ORF3</em>, <em>ORF4</em> and <em>ORF5</em>) at rice <em>S5</em> locus. The cooperation of <em>ORF5+</em> and <em>ORF4+</em> can result in endoplasmic reticulum (ER) stress and sporophytically kill all embryo sacs, while <em>ORF3+</em> can gametophytically protect the residing embryo sac. We previously identified four <em>S5-</em>interacting genes (<em>SIGs</em>) using a transgenic line BL^{<em>ORF5</em>}^<em>+</em> (Balilla carrying transgenic <em>ORF5+</em>) and a wide compatibility variety Dular (DL or D). Homozygote and hemizygote of <em>ORF5+</em> transgene had significantly different spikelet fertility (SF), which disturbed the phenotypic effects of <em>SIGs</em>. However, HS effects of <em>SIGs</em> under the endogenous (nontransgenic) gamete killer remained unknown. We formerly constructed a semisterile near isogenic line (NIL) <em>S5</em>-BL/NJ by introgressing <em>S5</em> fragment of <em>indica</em> rice Nanjing11 (NJ or N, carrying <em>ORF3+ORF4-ORF5+</em> haplotypes) into the genome of <em>japonica</em> rice Balilla (BL or B, carrying <em>ORF3-ORF4+ORF5-</em> haplotypes). The gamete-protecting effect of <em>ORF3+</em> in NJ may confuse SF effect of the <em>SIGs</em>, so we knocked out <em>ORF3+</em> of <em>S5</em>-NJ/NJ and crossed it with BL to get gamete-killing <em>S5</em>-BL/NJ^{<em>ΔORF3+</em>}<em>,</em> which can kill all (KA) gametes (abbreviated as enS5KA). Compared with the ex<em>S5</em>KA line (a NIL carrying <em>ORF5+</em> transgenic, wihch can kill all gamete), the en<em>S5</em>KA line conferred <em>SIGs</em> a more pronounced SF effect. The en<em>S5</em>KA<em>,SIG</em>-DDDD (four <em>SIGs</em> carry homozygous DL alleles) genotype caused a SF of about 78 %, while SF of ex<em>S5</em>KA<em>,SIG</em>-DDDD was only about 62 %. Moreover, all <em>SIGs</em> acted in a sporophytic manner without segregation distortion of genotype. Although en<em>S5</em>KA<em>,SIG</em>-DDDD plants had high SF, the ER stress still existed. The ovule section revealed that en<em>S5</em>KA,<em>SIG</em>-BBBB genotype (four <em>SIGs</em> carry homozygous BL allele, with ER stress and SF &lt; 5 %) caused abnormal degradation of nucellar cells and functional megaspores. In contrast, en<em>S5</em>KA,<em>SIG</em>-DDDD genotype preserved most nucellar cells and functional megaspores. These results lay the foundation for further research on HS mechanism of <em>S5</em> and <em>SIGs</em> and cloning of candidate genes.</div></div>","PeriodicalId":20273,"journal":{"name":"Plant Science","volume":"352 ","pages":"Article 112357"},"PeriodicalIF":4.2,"publicationDate":"2024-12-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142829554","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Nanobiotechnology for efficient plum pox virus elimination from apricot plants","authors":"C. Pérez-Caselles ,&nbsp;N. Alburquerque ,&nbsp;M. Martín-Valmaseda ,&nbsp;F.J. Alfosea-Simón ,&nbsp;L. Faize ,&nbsp;N. Bogdanchikova ,&nbsp;A. Pestryakov ,&nbsp;L. Burgos","doi":"10.1016/j.plantsci.2024.112358","DOIUrl":"10.1016/j.plantsci.2024.112358","url":null,"abstract":"<div><div>Metallic nanoparticles have antimicrobial, virucidal, and anticancer activities and have been widely applied in medicine. In plants, silver nanoparticles have been used as preventive treatments in the greenhouse to reduce viral titers and symptoms. This work investigates the effect of Argovit™ AgNP formulation on apricot plants infected with <em>Plum pox virus</em> or with <em>Hop stunt viroid</em>. Meristems were rescued from plants treated with different Argovit™ concentrations and two exposure times. Although viroid-free plants were not obtained, a very high efficiency in eliminating the sharka virus is reported with a maximum cleaning efficiency (75 %) after 8 weeks of exposure at 75 mg L⁻¹ AgNP. To the best of our knowledge, this is the first time that silver nanoparticles application is used, combined with meristem culture, to produce virus-free plants and opens a new path to the elimination of viruses from plants.</div></div>","PeriodicalId":20273,"journal":{"name":"Plant Science","volume":"352 ","pages":"Article 112358"},"PeriodicalIF":4.2,"publicationDate":"2024-12-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142829572","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Overexpression of ZmEULD1b enhances maize seminal root elongation and drought tolerance","authors":"Qian Lan ,&nbsp;Guanhua He ,&nbsp;Dongmei Wang ,&nbsp;Shen Li ,&nbsp;Yufeng Jiang ,&nbsp;Honghui Guan ,&nbsp;Yongxiang Li ,&nbsp;Xuyang Liu ,&nbsp;Tianyu Wang ,&nbsp;Yu Li ,&nbsp;Dengfeng Zhang ,&nbsp;Chunhui Li","doi":"10.1016/j.plantsci.2024.112355","DOIUrl":"10.1016/j.plantsci.2024.112355","url":null,"abstract":"<div><div>Drought stress damages the growth and development of maize, which results in the maize yield reduction. A strong root system improves the drought tolerance in maize. A previous genome-wide association study for the maize seminal root length under drought stress conditions identified a significant SNP, which was located in the <em>ZmEULD1b</em> gene. Here, we show that enhancing <em>ZmEULD1b</em> expression in transgenic maize increases seminal root length, as well as plant tolerance to water deficit. Meanwhile, <em>ZmEULD1b</em> overexpression influences the stomatal development and promotes water-use efficiency of maize. Further, transcriptome analysis of wild type and <em>ZmEULD1b-OE</em> plants show that several peroxidases and ABA-related pathway genes are upregulated in the <em>ZmEULD1b-OE</em> plants under drought stress conditions. Additionally, rhizosphere microbiota analyses of plant root confirm that overexpression of <em>ZmEULD1b</em> improves the abundance of growth-promoting microbes in the maize root system under drought stress conditions. Collectively, the data presented in this work suggest that <em>ZmEULD1b</em> could be a valuable gene resource or selection target for the drought-tolerant genetic improvement of maize.</div></div>","PeriodicalId":20273,"journal":{"name":"Plant Science","volume":"352 ","pages":"Article 112355"},"PeriodicalIF":4.2,"publicationDate":"2024-12-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142822624","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"ACS2 and ACS6, especially ACS2 is involved in MPK6 evoked production of ethylene under Cd stress, which exacerbated Cd toxicity in Arabidopsis thaliana","authors":"Chun Yan Tu ,&nbsp;Lu Zheng ,&nbsp;Jing Yan ,&nbsp;Ren Fang Shen ,&nbsp;Xiao Fang Zhu","doi":"10.1016/j.plantsci.2024.112354","DOIUrl":"10.1016/j.plantsci.2024.112354","url":null,"abstract":"<div><div>As one of the heavy metal pollutants with strong biological toxicity, cadmium (Cd) is easily absorbed by plant roots, which seriously restricts the growth of plants, causes the quality of agricultural products to decline and threatens human health. Many complex signal transduction pathways are involved in the process of plant response to Cd stress. Among them, plant hormone ethylene is an important signal molecule for plant response to various environmental stresses, and its regulatory mechanism and signal transduction pathway in Cd stress response need to be further clarified. Here, we discovered that Cd stress induced a significant increment in ethylene production in Arabidopsis roots, and the amount of ethylene produced was positively correlated with the inhibition of Arabidopsis root growth and Cd accumulation. Simultaneously, Cd stress stimulated the detoxification mechanism within cells and promoted the expression of <em>METAL TOLERANCE PROTEIN 3</em> (<em>MTP3</em>), <em>IRON-REGULATED TRANSPORTER2</em> (<em>IRT2</em>), <em>IRON REGULATED GENE 2</em> (<em>IREG2</em>) genes implicated in Cd vacuolar compartmentation. However, whether this is associated with ethylene signal transduction remains to be further explored. Further studies have revealed that the Cd induced ethylene burst is attributed to the up-regulation of the expression of <em>1-AMINOCYCLOPROPANE-1-CARBOXYLIC ACID SYNTHASE</em> (<em>ACS</em>) genes that mediated by MITONGEN-ACTIVATED PROTEIN KINASE 6 (MAPK6) in Arabidopsis roots, and among them, ACS2 and ACS6, especially ACS2, are involved in MAPK6-induced ethylene production under Cd stress. The results of this study provide new ideas for understanding the signal transduction pathway of plant response to Cd stress.</div></div>","PeriodicalId":20273,"journal":{"name":"Plant Science","volume":"352 ","pages":"Article 112354"},"PeriodicalIF":4.2,"publicationDate":"2024-12-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142822646","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The role of interplay between the plant plasma membrane H+-ATPase and its lipid environment","authors":"Nikita K. Lapshin,&nbsp;Marina S. Trofimova","doi":"10.1016/j.plantsci.2024.112343","DOIUrl":"10.1016/j.plantsci.2024.112343","url":null,"abstract":"<div><div>The mechanisms behind the regulation of plasma membrane (PM) P-type H⁺-ATPase in plant cells mediated by lipid-protein interactions and lateral heterogeneity of the plasma membrane are discussed. This review will focus on 1) the structural organization and mechanisms of the catalytic cycle of the enzyme, 2) phosphorylation as the primary mechanism of pump regulation; 3) the possible role of lateral heterogeneity of the plasma membrane in this process, as well as 4) the role of lipids in the H⁺-ATPase biosynthesis and its delivery to the plasma membrane. In addition, 5) the potential role of membrane lipids in the H⁺-ATPase co-localisation with secondary active transporters is speculated.</div></div>","PeriodicalId":20273,"journal":{"name":"Plant Science","volume":"352 ","pages":"Article 112343"},"PeriodicalIF":4.2,"publicationDate":"2024-12-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142786745","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Hydrogen gas enhances Arabidopsis salt tolerance by modulating hydrogen peroxide-mediated redox and ion homeostasis","authors":"Linlin Xiao ,&nbsp;Yueran Hu ,&nbsp;Yiting Wang ,&nbsp;Chengsi Lv ,&nbsp;Na Zhan ,&nbsp;Hongying Duan ,&nbsp;Jiuchang Su","doi":"10.1016/j.plantsci.2024.112356","DOIUrl":"10.1016/j.plantsci.2024.112356","url":null,"abstract":"<div><div>Hydrogen gas (H₂) plays a crucial role in mitigating salt stress in plants, but the underlying mechanisms is largely unknown. Herein, we employed the pharmacological, molecular, and genetic approaches to investigate the positive roles of hydrogen peroxide (H₂O₂) in endogenous H₂-induced salt tolerance of <em>Arabidopsis thaliana</em>. H₂-induecd salt tolerance of <em>CrHYD1</em> (hydrogenase 1 gene from <em>Chlamydomonas reinhardtii</em>) transgenic <em>Arabidopsis</em> was blocked by H₂O₂ scavenger or NADPH oxidase inhibitor. When RESPIRATORY BURST OXIDASE HOMOLOG (RBOH) genes (<em>AtrbohD</em> or <em>AtrbohF</em>) were mutated, salt sensitivity of <em>CrHYD1/atrboh</em> (especially <em>CrHYD1/atrbohD</em>) hybrids was increased, but diminished by exogenous H₂O₂ administration. Salt-stimulated endogenous H₂ enrichment consequently resulted in the rapid reactive oxygen species (ROS) accumulation under early salt stress, and the expression of <em>AtrbohD</em> (especially) and <em>AtrbohF</em> in <em>CrHYD1</em> plants was higher than those in the wild-type (WT), suggesting that endogenous H₂ could induce Atrboh-dependent ROS burst to respond salt stress. Further, H₂-induced less 3,3′-diaminobenzidine (DAB) and nitro blue tetrazolium (NBT) stain in <em>CrHYD1</em> plants was reversed under salt stress when either H₂O₂ was removed or <em>Atrboh</em>s were mutated, which could be explained by higher H₂O₂ and thiobarbituric acid reactive substances (TBARS) levels, as well as lower antioxidant enzyme activity. Additionally, H₂-induced Na⁺ discharge and K⁺ accumulation in <em>CrHYD1</em> plants under salt stress were blocked by either H₂O₂ removal or <em>Atrboh</em> knockout, which was validated by higher Na⁺/K⁺ ratios and lower ion transport-related gene expression. Our findings not only elucidate that endogenous H₂ enhanced <em>Arabidopsis</em> salt tolerance by reestablishing H₂O₂-dependent ion and redox homeostasis, but provide new insights into the mechanisms of plant salinity responses.</div></div>","PeriodicalId":20273,"journal":{"name":"Plant Science","volume":"352 ","pages":"Article 112356"},"PeriodicalIF":4.2,"publicationDate":"2024-12-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142802067","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Leaf trait networks of subtropical woody plants weaken along an elevation gradient","authors":"Yiwei Ren ,&nbsp;Jinlong Li ,&nbsp;Shudong Zhang ,&nbsp;Jing Shao ,&nbsp;Xueqin Li ,&nbsp;Quanlin Zhong ,&nbsp;Dandan Hu ,&nbsp;Dongliang Cheng","doi":"10.1016/j.plantsci.2024.112340","DOIUrl":"10.1016/j.plantsci.2024.112340","url":null,"abstract":"<div><div>The leaf economic spectrum (LES) captures key leaf functional trait relationships, defining a conservative-acquisitive axis of plant resource utilization strategies. Examining the leaf trait network (LTN) is useful for understanding resource utilization strategies but also more broadly, the ecological strategies of plants. However, the relationship between the LES conservation-acquisition axis and LTN correlations across environmental gradients is unclear. To address this knowledge gap, we measured physiological, chemical, and structural traits in 52 broad-leaved tree species spanning an elevation gradient (1400 m, 1600 m, 1800 m) in Wuyi Mountain, China. A total of 12 leaf traits were selected, including: photosynthetic rate (<em>A</em>₂₅), respiration rate (<em>R</em>₂₅), optimum photosynthetic temperature (<em>T</em>_opt), rate of photosynthesis at optimum temperature (<em>A</em>_opt), mean temperature at which 90 % of <em>A</em>_opt is reached (<em>T</em>₉₀), temperature sensitivity of respiration (<em>Q</em>₁₀), N and P content, N/P, leaf mass per area (LMA), photosynthetic nitrogen use efficiency (PNUE) and photosynthetic phosphorus use efficiency (PPUE). We found that leaf physiological traits exhibited signs of thermal acclimation along the elevation gradient. We also observed significant changes in leaf N and P content, N/P, photosynthetic phosphorus utilization efficiency (PPUE) and LMA with elevation. The resource utilization strategies of plants changed from conservative to acquisitive as elevation increased. The LTN analysis showed that as elevation increased, the links among traits weakened and modularity (modularity is used to describe the degree of separation between networks) increased. Collectively, our results indicate that elevation changes can trigger moderate shifts in the resource utilization and ecological strategies of plants via leaf functional traits.</div></div>","PeriodicalId":20273,"journal":{"name":"Plant Science","volume":"352 ","pages":"Article 112340"},"PeriodicalIF":4.2,"publicationDate":"2024-12-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142791707","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Evaluation of resistance to Verticillium wilt in Gossypium hirsutum-Gossypium arboreum introgression lines and identification of putative resistance genes using RNA-seq","authors":"Shuang Gao ,&nbsp;Susu Liu ,&nbsp;Guoli Feng ,&nbsp;Jianbo Gao ,&nbsp;Ningshan Wang ,&nbsp;Nijiang Ai ,&nbsp;Baoliang Zhou","doi":"10.1016/j.plantsci.2024.112353","DOIUrl":"10.1016/j.plantsci.2024.112353","url":null,"abstract":"<div><div>Verticillium wilt (VW), a fungal disease caused by <em>Verticillium dahliae</em> (<em>Vd</em>), is one of the most destructive threats to cotton production. Moreover, widely cultivated upland cotton (<em>Gossypium hirsutum</em>, 2<em>n</em> = 4<em>x</em> = AADD = 52) often demonstrates low resistance to <em>Vd</em>. In contrast, <em>G. arboreum</em> (2<em>n</em> = 2<em>x</em> = AA = 26) shows high resistant to VW, making it a valuable source for breeding, despite the challenges posed by hybridization incompatibility between the two species. Here, a population of introgression lines derived from <em>G. hirsutum</em> and <em>G. arboreum</em> was evaluated for resistance to VW through both glasshouse and field tests. Among these lines, DM11039 demonstrated high resistance to VW. Both DM11039 and the recipient TM-1 underwent transcriptome sequencing during <em>Vd</em> infection at 0, 4, 12, 24, 48, and 96 h post inoculation. The analysis identified differentially expressed genes (DEGs), which were predominantly associated with resistance mechanisms. Based on the results from transcriptome sequencing and weighted correlation network analysis, three DEGs from each parent—<em>G. arboreum</em> and <em>G. hirsutum</em>— in DM11039 were subjected to virus-induced gene silencing in cotton seedlings. The findings revealed that silencing of <em>GaPP2A1</em>, <em>GaPDH-E1</em>, or <em>GaLRK10L-1.2</em>, which are located within the introgression segments from <em>G. arboreum</em>, significantly impaired disease resistance in cotton. This suggests that these genes are potentially linked to the disease phenotype. In contrast, silencing of <em>GHA13G1263</em>, <em>GhZIP1</em> or <em>GHA10G2498</em> from <em>G. hirsutum</em> did not result in any changes in disease resistance in DM11039. The results indicate <em>G. arboreum</em> harbors resistance genes to VW. Furthermore, the introgression population presents a valuable resource for future cotton breeding.</div></div>","PeriodicalId":20273,"journal":{"name":"Plant Science","volume":"352 ","pages":"Article 112353"},"PeriodicalIF":4.2,"publicationDate":"2024-12-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142791528","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}