Plant Science最新文献_第7页

OsNCED1, a chloroplast ABA biosynthase, regulates multiple abiotic stress tolerance in rice 水稻叶绿体ABA生物合成酶OsNCED1调控多种非生物胁迫抗性。

IF 4.1 2区生物学 Q2 BIOCHEMISTRY & MOLECULAR BIOLOGY

Plant Science

Pub Date : 2026-01-04 DOI: 10.1016/j.plantsci.2026.112979

Zhipan Xiang , Minfeng Lu , Yuxian Yao , Mingze Zhang , Zhendong Zhang , Songmei Chen , Xishan Pan , Huizhou Fu , Lin Zhang

Abscisic acid (ABA)‌ is one of the most critical stress hormones in plants, yet the mechanisms underlying its biosynthesis pathway in regulating multiple abiotic stress tolerance remain poorly understood. In this study, we identified the function of 9-Cis-Epoxycarotenoid Dioxygenase 1 ‌(OsNCED1)‌, a key ABA biosynthetic enzyme gene in rice (Oryza sativa L.), in regulating multiple abiotic stresses (cold, salt, and osmotic stress) tolerance. OsNCED1 is a chloroplast-localized ABA synthase. Its expression is significantly induced by multiple abiotic stresses. Knockout of ‌OsNCED1‌ markedly impairs the tolerance of rice seedlings to cold, salt, and osmotic stresses, whereas exogenous ABA application restores the stress hypersensitivity of ‌osnced1‌ mutants. Overexpression of ‌OsNCED1‌ significantly enhances tolerance to multiple stresses. Physiological analyses indicate that OsNCED1 mediates stress tolerance through the maintenance of ABA homeostasis and reactive oxygen species (ROS) scavenging‌. The potential molecular mechanism suggests that OsNCED1 alters the expression of ABA signaling pathway genes in mutant and overexpression plants to regulate stress tolerance. Taken together, ‌OsNCED1 is a ‌positive regulator‌ of stress tolerance and represents a promising target gene for improving multiple abiotic stress tolerance in rice seedlings via molecular breeding strategies in the future.

脱落酸（ABA）是植物中最重要的胁迫激素之一，但其生物合成途径调控多种非生物胁迫耐受性的机制尚不清楚。在这项研究中，我们确定了9-顺式环氧类胡萝卜素双加氧酶1 (OsNCED1)，水稻（Oryza sativa L.）中一个关键的ABA生物合成酶基因，在调节多种非生物胁迫（冷、盐和渗透胁迫）耐受性中的功能。OsNCED1是叶绿体定位的ABA合成酶。它的表达受多种非生物胁迫的显著诱导。敲除OsNCED1的基因会显著削弱水稻幼苗对寒冷、盐和渗透胁迫的耐受性，而外源ABA的应用则会恢复OsNCED1突变体的胁迫敏感性。OsNCED1 -过表达可显著增强对多种胁迫的耐受性。生理分析表明，OsNCED1通过维持ABA稳态和清除活性氧（ROS）介导胁迫耐受性。潜在的分子机制提示OsNCED1通过改变ABA信号通路基因在突变体和过表达植物中的表达来调节胁迫耐受性。综上所述，OsNCED1是胁迫耐受性的一个正调节因子，是未来通过分子育种策略提高水稻幼苗多种非生物胁迫耐受性的一个有希望的靶基因。

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引用次数: 0

Brassinosteroids and TaGSK3 coordinate phosphorus uptake and low-phosphorus tolerance in wheat through overlapping and independent pathways 油菜素内酯和TaGSK3通过重叠和独立的途径协调小麦的磷吸收和低磷耐受

IF 4.1 2区生物学 Q2 BIOCHEMISTRY & MOLECULAR BIOLOGY

Plant Science

Pub Date : 2026-01-04 DOI: 10.1016/j.plantsci.2026.112978

Pengcheng Wang , Jialiang Zhang , Chen Yang , Yaxin Niu , Junzhe Wang , Wanquan Ji , Yan Li , Xiaoming Wang , Shengbao Xu , Xue Shi

Brassinosteroids (BRs) regulate plant growth and stress responses, but their role in wheat's phosphate (Pi) uptake and low-phosphorus (LP) tolerance is unclear. This study reveals that BR signaling enhances Pi uptake and LP tolerance by modulating the expression of key genes involved in Pi uptake, transport and LP response. Consistently, the sextuple mutants of TaGSK3, encoding the key repressor of the BR signaling, increased Pi uptake by 16.42 %. Unexpectedly, the TaGSK3 gain-of-function mutants also exhibited a 129.90–202.30 % increase in Pi uptake, mediated by their increased kinase activity. Under LP stress, sextuple mutants of TaGSK3 exhibited enhanced tillering and root elongation, whereas TaGSK3 gain-of-function mutants increased root biomass, suggesting the functional state of TaGSK3 affects the trade-off strategies for above-ground and below-ground growth. Overall, these results uncover a complex regulatory network in which BR signaling and TaGSK3 collaboratively and independently coordinate Pi homeostasis, offering novel targets for improving phosphorus use efficiency in wheat.

油菜素内酯（BRs）调节植物生长和胁迫反应，但其在小麦磷素（Pi）吸收和低磷（LP）耐受性中的作用尚不清楚。该研究表明，BR信号通过调节参与Pi摄取、转运和LP反应的关键基因的表达来增强Pi摄取和LP耐受性。同样，编码BR信号关键抑制因子的TaGSK3的六重突变体增加了16.42%的Pi摄取。出乎意料的是，TaGSK3功能获得突变体也表现出129.90-202.30%的Pi摄取增加，这是由它们增加的激酶活性介导的。在LP胁迫下，6个突变体TaGSK3的分蘖和根伸长增强，而TaGSK3的功能获得突变体则增加了根生物量，表明TaGSK3的功能状态影响了地上和地下生长的权衡策略。总的来说，这些结果揭示了一个复杂的调控网络，其中BR信号和TaGSK3协同或独立地协调Pi稳态，为提高小麦磷利用效率提供了新的靶点。

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引用次数: 0

Stress-associated proteins (SAPs): Molecular hubs connecting plant stress, development, and metabolism 胁迫相关蛋白：连接植物胁迫、发育和代谢的分子枢纽。

IF 4.1 2区生物学 Q2 BIOCHEMISTRY & MOLECULAR BIOLOGY

Plant Science

Pub Date : 2026-01-03 DOI: 10.1016/j.plantsci.2026.112976

Yashi Wen , Qing Yang , Zhijing Yu , Jingying Liu , Bingbing Lv

Stress-associated proteins (SAPs) are a versatile class of proteins extensively found in microbes, plants, and animals. They play a pivotal role in plant responses to environmental challenges such as drought, salinity, heat, and pathogen infection, thereby enhancing stress tolerance and safeguarding agricultural yields. Current research has largely focused on the stress-responsive functions of SAPs, while the connections between their diverse roles and practical applications remain less synthesized. To advance the understanding of the SAPs family and facilitate for future research into stress-tolerant crop breeding aimed at reducing yield losses, this paper comprehensively examines the classification, structural traits, molecular mechanisms, and application potential of SAPs in stress regulation.

应激相关蛋白（SAPs）是广泛存在于微生物、植物和动物中的一类多用途蛋白质。它们在植物对干旱、盐、热和病原体感染等环境挑战的响应中发挥关键作用，从而增强抗逆性，保障农业产量。目前的研究主要集中在sap的应力响应功能上，而sap的多种作用与实际应用之间的联系还不够全面。本文对SAPs家族的分类、结构特征、分子机制及其在逆境调控中的应用潜力进行了综述，为进一步深入了解SAPs家族，开展以减少作物产量损失为目标的抗逆性作物育种提供参考。

引用次数: 0

The papain-like cysteine proteases (PLCP) in tomato: Identification, expression analysis, and functional characterization of SlRD19B under salt stress 盐胁迫下番茄木瓜样半胱氨酸蛋白酶SlRD19B的鉴定、表达分析及功能表征

IF 4.1 2区生物学 Q2 BIOCHEMISTRY & MOLECULAR BIOLOGY

Plant Science

Pub Date : 2026-01-02 DOI: 10.1016/j.plantsci.2026.112974

Jiaxuan Zhu, Ruirui Yang, Yan Li, Ruili Lv, Huimin Li, Yao Yao, Yushi Luan

Papain-like cysteine proteases (PLCPs) are key enzymes involved in protein hydrolysis and play critical roles in plant growth, development, and responses to stresses. Although PLCPs have been systematically identified in various plant species, their functions in tomato remain largely unexplored, particularly their roles in salt stress adaptation. In this study, we identified 32 PLCP genes in the tomato genome and classified them into nine subfamilies. We found that the promoter regions of SlPLCP genes are enriched with stress-responsive elements. Combining transcriptome data and qRT-PCR analysis showed that SlRD19B was the most significantly upregulated gene under salt stress. Further functional studies demonstrated that silencing SlRD19B enhanced tomato sensitivity to salt stress, as evidenced by severe leaf wilting, increased membrane damage, reduced osmotic adjustment capacity, inhibited root growth, and an elevated Na⁺/K⁺ ratio. Additionally, SlRD19B-silenced plants exhibited excessive reactive oxygen species (ROS) accumulation and disrupted antioxidant enzyme activities under salt stress, characterized by increased superoxide dismutase (SOD) and catalase (CAT) activities but decreased peroxidase (POD) activity. This study systematically reveals the crucial role of the SlPLCP gene family in tomato salt stress response and provides new insights into the functional mechanisms of PLCP genes in plants.

木瓜素样半胱氨酸蛋白酶（PLCPs）是参与蛋白质水解的关键酶，在植物生长发育和逆境响应中起着关键作用。虽然PLCPs已经在不同的植物物种中被系统地鉴定出来，但它们在番茄中的功能，特别是在盐胁迫适应中的作用仍未得到充分的研究。在这项研究中，我们在番茄基因组中鉴定了32个PLCP基因，并将它们分为9个亚科。我们发现SlPLCP基因的启动子区域富含应激响应元件。结合转录组数据和qRT-PCR分析发现，SlRD19B是盐胁迫下表达上调最显著的基因。进一步的功能研究表明，沉默SlRD19B增强了番茄对盐胁迫的敏感性，表现为叶片严重萎蔫、膜损伤增加、渗透调节能力降低、根生长受到抑制、Na + /K +比值升高。此外，slrd19b沉默植株在盐胁迫下活性氧（ROS）积累过多，抗氧化酶活性被破坏，表现为超氧化物歧化酶（SOD）和过氧化氢酶（CAT）活性升高，过氧化物酶（POD）活性降低。本研究系统揭示了SlPLCP基因家族在番茄盐胁迫响应中的重要作用，为PLCP基因在植物中的作用机制提供了新的认识。

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引用次数: 0

Genome-wide identification and expression analyses reveal cell wall degradation gene family networks in kiwifruit softening 全基因组鉴定和表达分析揭示了猕猴桃软化过程中细胞壁降解基因家族网络

IF 4.1 2区生物学 Q2 BIOCHEMISTRY & MOLECULAR BIOLOGY

Plant Science

Pub Date : 2025-12-31 DOI: 10.1016/j.plantsci.2025.112972

Zhan Shi , Shenzhong Gao , Fabi Shen , Han Yang , Yongsheng Liu , Yinan Yao , Jikai Liu , Lijun Wang , Yongfeng Gao , Peng Shu , Heng Deng

Understanding the molecular mechanisms underlying fleshy fruit softening holds significant biological importance, as it provides critical insights for enhancing fruit quality, nutritional value, and shelf life while reducing postharvest losses. Although key structural genes associated with softening have been extensively characterized in model fleshy fruits, the gene families regulating kiwifruit (Actinidia chinensis) softening remain incompletely elucidated. Here, we performed genome-wide analyses to identify nine cell wall-related gene families potentially involved in fruit softening in kiwifruit: PL, BXL, CEL, EXP, MAN, PG, PME, TBG/BGAL, and XTH. Systematic phylogenetic, gene structure, and collinear homology analyses, combined with expression profiling and regulatory network construction, revealed the functional divergence of these gene families. Focusing specifically on the PL gene family, we applied AlphaFold3 to generate large-scale predictions of interactions between TF and SG interactions in the regulatory network. High-confidence interactions were selected via rigorous scoring, and the network was optimized with expression profiling. Electrophoretic mobility shift (EMSA) and luciferase reporter (LUC) assays confirmed the network-predicted transcriptional regulation of AcPL26 by AcERF015. Moreover, transient expression of AcERF015 in kiwifruit markedly reduced fruit firmness and elevated water loss rate, accompanied by a significant up-regulation of AcPL26 transcript levels as revealed by RT-qPCR, as well as increased pectate lyase activity and reduced protopectin content, collectively supporting the predictions of the regulatory network. Our findings provide pathway regulatory insights into the PCW degradation gene families governing kiwifruit softening and support improvement strategies.

了解肉质水果软化的分子机制具有重要的生物学意义，因为它为提高水果质量、营养价值和保质期提供了重要的见解，同时减少采后损失。虽然在模式肉质果实中与软化相关的关键结构基因已被广泛研究，但调控猕猴桃软化的基因家族仍未完全阐明。在这里，我们进行了全基因组分析，以确定9个细胞壁相关基因家族可能参与猕猴桃果实软化：PL、BXL、CEL、EXP、MAN、PG、PME、TBG/BGAL和XTH。系统的系统发育、基因结构和共线同源性分析，结合表达谱和调控网络构建，揭示了这些基因家族的功能差异。针对PL基因家族，我们应用AlphaFold3对调控网络中TF和SG之间的相互作用进行大规模预测。通过严格的评分选择高置信度的交互，并通过表达谱优化网络。电泳迁移转移（EMSA）和荧光素酶报告基因（LUC）检测证实了AcERF015对AcPL26的网络预测转录调控。此外，AcERF015在猕猴桃中的瞬时表达显著降低了果实硬度和水分损失率，RT-qPCR结果显示，AcPL26转录物水平显著上调，果胶裂解酶活性升高，原生蛋白含量降低，共同支持了调控网络的预测。我们的研究结果为控制猕猴桃软化的PCW降解基因家族提供了途径调控的见解，并支持了改进策略。

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引用次数: 0

The plastidial phosphorylase Pho1 co-localizes in situ with Photosystem I-associated PsaC as assessed by double labeling immunocytochemistry – transmission electron microscopy 通过双标记免疫细胞化学-透射电镜评估，Pho1与光系统1相关的PsaC在原位共定位。

IF 4.1 2区生物学 Q2 BIOCHEMISTRY & MOLECULAR BIOLOGY

Plant Science

Pub Date : 2025-12-31 DOI: 10.1016/j.plantsci.2025.112953

Masako Fukuda , Chun-yeung Ng , Seon-Kap Hwang , Thomas W. Okita

The plastidial phosphorylase Pho1 is essential for normal starch biosynthesis in cereal grains. Biochemical studies, however, have shown that this starch biosynthetic enzyme interacts with PsaC, the terminal electron acceptor–donor protein of Photosystem I (PSI). This association with PsaC associated with PSI is supported by the localization of Pho1 to thylakoid membranes enriched in PSI complexes. To obtain direct in situ evidence for this protein–protein interaction within thylakoid membranes, we employed double-label immunocytochemistry at the electron microscopy level. Thin sections of leaf tissue from transgenic rice lines expressing GFP-tagged PsaC, as well as from wild-type plants, were incubated with mouse anti-GFP and rabbit anti-Pho1 antibodies, followed by secondary labeling with 6 nm and 12 nm gold particles, respectively, to visualize the antigen–antibody complexes. Despite the inherently low efficiency of immunogold labeling due to reduced antigenicity from chemical fixation and limited antibody penetration in resin-embedded tissue, we observed paired gold particles—at frequencies significantly higher than random—in which Pho1 was located in close proximity to GFP–PsaC. These findings support the direct interaction of Pho1 with PSI bound PsaC and, therefore, provide further justification to investigate the structural and functional basis of Pho1’s influence on PSI activity.

在谷物中，可塑性磷酸化酶Pho1是正常淀粉生物合成所必需的。然而，生化研究表明，这种淀粉生物合成酶与光系统I （PSI）的终端电子受体-供体蛋白PsaC相互作用。Pho1定位到富含PSI复合物的类囊体膜上，支持了PS1与PsaC的关联。为了获得类囊体膜内蛋白质-蛋白质相互作用的直接原位证据，我们在电镜水平上采用了双标记免疫细胞化学。用小鼠抗gfp抗体和兔抗pho1抗体孵育表达gfp标记的PsaC的转基因水稻株系和野生型植株的叶片组织薄片，然后分别用6nm和12nm的金颗粒进行二次标记，以观察抗原-抗体复合物。尽管由于化学固定的抗原性降低和树脂包埋组织中有限的抗体渗透，免疫金标记的效率本来就很低，但我们观察到配对金粒子的频率显著高于随机频率，其中Pho1位于GFP-PsaC附近。这些发现支持了Pho1与PSI结合的PsaC的直接相互作用，因此为进一步研究Pho1影响PSI活性的结构和功能基础提供了依据。

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引用次数: 0

“Alternaria radicina xylanase is required for the occurrence of carrot black rot disease” [Plant Sci. 359 (2025) 112582] “胡萝卜黑腐病的发生需要根瘤菌木聚糖酶”[植物科学，359(2025)112582]。

IF 4.1 2区生物学 Q2 BIOCHEMISTRY & MOLECULAR BIOLOGY

Plant Science

Pub Date : 2025-12-30 DOI: 10.1016/j.plantsci.2025.112955

Yingying Wang , Zhimin Wang , Shuo Liu , Jianxin Deng , Ge Wang , Fangfang Ma , Zhilong Bao

引用次数: 0

Role of beneficial metalloids, silicon and selenium, in enhancing yield and quality in floricultural crops 有益金属硅和硒在提高花卉作物产量和品质中的作用

IF 4.1 2区生物学 Q2 BIOCHEMISTRY & MOLECULAR BIOLOGY

Plant Science

Pub Date : 2025-12-30 DOI: 10.1016/j.plantsci.2025.112971

Nikita S. Gawade , Madhuri C. Pagariya , Pritam R. Jadhav , Murshid Muhammed PK , Nalini A. Shinde , Rushil Mandlik , S. Madhavan , Pravin V. Jadhav , Humira Sonah , Gunvant B. Patil , Prashant G. Kawar

Micronutrients, although required in low concentrations, are key in the plant’s overall development and stress mitigation. Micronutrients can be either metals, non-metals or metalloids. Beneficial metalloids, such as silicon and selenium, enhance plant growth while also helping the plant combat various stresses, including drought, salinity, temperature stress, nutrient toxicity, pathogens, and herbivory. Floricultural crops are essential from an economic perspective, and thus their yield, as well as quality, need to be improved to increase the commercialisation of these crops. The effects of these two beneficial metalloids have been examined for various agricultural and non-agricultural crops; however, floricultural crops have been explored to a limited extent in this aspect. This review aims to elucidate the role of silicon and selenium in plant growth by understanding their uptake, transport, and deposition. Furthermore, as these metalloids are well-known for their role in stress alleviation, we discuss their potential role in influencing phytohormones, either directly or indirectly. Optimal amounts of these metalloids in the soil/substrate for floral crops dictate the enhancement of floral attributes, such as yield, shelf life, and management of biotic and abiotic stress. Understanding the role of silicon and selenium supplementation in floricultural crops offers a fresh perspective for research, leading to advancements in this area and ultimately improving these crops.

微量营养素虽然需要低浓度，但对植物的整体发育和缓解胁迫至关重要。微量营养素可以是金属、非金属或类金属。有益的类金属，如硅和硒，促进植物生长，同时也帮助植物对抗各种胁迫，包括干旱、盐度、温度胁迫、营养毒性、病原体和草食。从经济角度来看，花卉作物是必不可少的，因此需要提高它们的产量和质量，以增加这些作物的商业化。研究了这两种有益的类金属对多种农业和非农业作物的影响；然而，花卉作物在这方面的探索还很有限。本文旨在通过对硅和硒在植物生长中的吸收、转运和沉积的了解来阐明它们在植物生长中的作用。此外，由于这些类金属以其在缓解应激中的作用而闻名，我们讨论了它们直接或间接影响植物激素的潜在作用。花卉作物土壤/基质中这些类金属的最佳含量决定了花卉属性的提高，如产量、保质期以及对生物和非生物胁迫的管理。了解硅和硒补充在花卉作物中的作用为研究提供了一个新的视角，导致该领域的进步，并最终改善这些作物。

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引用次数: 0

Integrated multi-omics reveals the interactive mechanisms of antioxidant defense, energy metabolism, and programmed cell death in cadmium tolerance of Elymus nutans Griseb. 综合多组学研究揭示了羊草耐镉性中抗氧化防御、能量代谢和细胞程序性死亡的相互作用机制。

IF 4.1 2区生物学 Q2 BIOCHEMISTRY & MOLECULAR BIOLOGY

Plant Science

Pub Date : 2025-12-29 DOI: 10.1016/j.plantsci.2025.112969

Mengli Han , Ruxue Sang , Ruihan Mao , Min Pan , Yuxia Guo , Zhao Chen , Xuebing Yan

Cadmium (Cd), a non-essential toxic heavy metal, presents severe threats to agricultural productivity, plant development, and human health through food chain contamination. Although Elymus nutans Griseb. (E. nutans) exhibits substantial potential for phytoremediation of Cd-contaminated soils, its molecular Cd-tolerance mechanisms remain poorly characterized. This study employed integrated morphological, physiological, ultrastructural, transcriptomic, and metabolomic analyses to systematically investigate Cd stress responses in E. nutans. Results demonstrated that Cd stress significantly inhibited growth and photosynthesis, activated antioxidant system, and induced lipid peroxidation in E. nutans seedlings. Notably, cellular ultrastructural damage coincided with disrupted energy metabolism and substantial accumulation of reactive oxygen species (ROS), which ultimately triggered programmed cell death (PCD). Transcriptomic analysis revealed differentially expressed genes (DEGs) enriched in Plant hormone signal transduction and Carbon metabolism pathways. Metabolomic profiling identified 128 differentially expressed metabolites (DEMs) associated with ABC transporters and Isoquinoline alkaloid biosynthesis. Integrated multi-omics analysis demonstrated that glutathione metabolism and photosynthetic carbon fixation pathways regulate E. nutans response to Cd by modulating photosynthesis, antioxidant defense, and energy metabolism. This study reveals phytoremediation mechanisms of E. nutans under Cd stress, offering a scientific foundation for restoring soil health and ecological functions in high-altitude contaminated areas through sustainable soil management strategies.

镉（Cd）是一种非必需的有毒重金属，通过食物链污染对农业生产力、植物发育和人类健康构成严重威胁。虽然Elymus nutans Griseb。（E. nutans）显示出cd污染土壤的植物修复潜力，但其分子cd耐受机制尚不清楚。本研究采用综合形态学、生理学、超微结构、转录组学和代谢组学分析，系统地研究了花生对镉胁迫的反应。结果表明，Cd胁迫显著抑制了花生幼苗的生长和光合作用，激活了抗氧化系统，诱导了脂质过氧化。值得注意的是，细胞超微结构损伤与能量代谢紊乱和活性氧（ROS）的大量积累同时发生，最终引发程序性细胞死亡（PCD）。转录组学分析揭示了植物激素信号转导和碳代谢途径中富集的差异表达基因（DEGs）。代谢组学分析鉴定了128种与ABC转运蛋白和异喹啉生物碱生物合成相关的差异表达代谢物（dem）。综合多组学分析表明，谷胱甘肽代谢和光合固碳途径通过调节光合作用、抗氧化防御和能量代谢来调节花生对Cd的反应。本研究揭示了镉胁迫下花生的植物修复机制，为通过土壤可持续管理策略恢复高海拔污染地区土壤健康和生态功能提供科学依据。

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引用次数: 0

Engineering Arabidopsis for improved yield and nutritional quality through heterologous expression of maize ZmERF118 gene 利用玉米ZmERF118基因的异源表达改造拟南芥提高产量和营养品质。

IF 4.1 2区生物学 Q2 BIOCHEMISTRY & MOLECULAR BIOLOGY

Plant Science

Pub Date : 2025-12-29 DOI: 10.1016/j.plantsci.2025.112968

Zongjie Wang , Yang Zhao , Xinrui Ma , Yanjun Dong , Manyao Liu , Peng Jiao , Kaisheng Yu , Yimeng Wang , Yiyong Ma , Shuyan Guan

As one of the world's three major staple crops, maize is indispensable, particularly because of the critical role its essential amino acids play in both human and livestock nutrition. This study investigated the function of ZmERF118 in seed amino acid accumulation in Columbia wild-type (Col-0 WT) Arabidopsis plants. Subcellular localization experiments in tobacco leaves revealed that ZmERF118 acts as a transcription factor localized in the plant nucleus and encodes a 25.6 kDa protein. Compared with Col-0 WT Arabidopsis, overexpression of this gene promoted seedling growth and increased seed size but inhibited embryo development in the seeds. Moreover, this gene positively regulated amino acid content in Arabidopsis seeds, with significantly elevated levels of all amino acids, except tryptophan, compared with the WT. In addition, the seed starch content was significantly lower than that of WT. In summary, overexpression of the ZmERF118 positively regulated amino acid content and negatively regulated starch content in Arabidopsis seeds.

作为世界三大主粮作物之一，玉米是不可或缺的，特别是因为其必需氨基酸在人类和牲畜营养中发挥着关键作用。本研究研究了ZmERF118在哥伦比亚野生型（Col-0 WT）拟南芥种子氨基酸积累中的作用。烟草叶片亚细胞定位实验表明，ZmERF118作为转录因子定位于植物细胞核，编码一个25.6kDa的蛋白。与Col-0 WT拟南芥相比，过表达该基因可促进幼苗生长，增大种子大小，但抑制种子胚发育。此外，该基因正调控拟南芥种子中氨基酸含量，除色氨酸外，其余氨基酸含量均显著高于WT，籽粒淀粉含量显著低于WT。综上所述，过表达ZmERF118基因正调控拟南芥种子中氨基酸含量，负调控淀粉含量。

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引用次数: 0