Pub Date : 2025-01-26DOI: 10.1016/j.plantsci.2025.112399
Yanli Yang , Yi Xu , Baozhen Feng , Peiqian Li , Chengqi Li , Chen-Yu Zhu , Shu-Ning Ren , Hou-Ling Wang
Abiotic stresses adversely impact plants survival and growth, which in turn affect plants especially crop yields worldwide. To cope with these stresses, plant responses depend on the activation of molecular networks cascades, including stress perception, signal transduction, and the expression of specific stress-related genes. Plant bZIP (basic leucine zipper) transcription factors are important regulators that respond to diverse abiotic stresses.By binding to specific cis-elements, bZIPs can control the transcription of target genes, giving plants stress resistance. This review describes the structural characteristics of bZIPs and summarizes recent progress in analyzing the molecular mechanisms regulating plant responses to salinity, drought, and cold in different plant species. The main goal is to deepen the understanding of bZIPs and explore their value in genetic improvement of plants.
{"title":"Regulatory networks of bZIPs in drought, salt and cold stress response and signaling","authors":"Yanli Yang , Yi Xu , Baozhen Feng , Peiqian Li , Chengqi Li , Chen-Yu Zhu , Shu-Ning Ren , Hou-Ling Wang","doi":"10.1016/j.plantsci.2025.112399","DOIUrl":"10.1016/j.plantsci.2025.112399","url":null,"abstract":"<div><div>Abiotic stresses adversely impact plants survival and growth, which in turn affect plants especially crop yields worldwide. To cope with these stresses, plant responses depend on the activation of molecular networks cascades, including stress perception, signal transduction, and the expression of specific stress-related genes. Plant bZIP (basic leucine zipper) transcription factors are important regulators that respond to diverse abiotic stresses.By binding to specific <em>cis</em>-elements, bZIPs can control the transcription of target genes, giving plants stress resistance. This review describes the structural characteristics of bZIPs and summarizes recent progress in analyzing the molecular mechanisms regulating plant responses to salinity, drought, and cold in different plant species. The main goal is to deepen the understanding of bZIPs and explore their value in genetic improvement of plants.</div></div>","PeriodicalId":20273,"journal":{"name":"Plant Science","volume":"352 ","pages":"Article 112399"},"PeriodicalIF":4.2,"publicationDate":"2025-01-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143060428","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2025-01-21DOI: 10.1016/j.plantsci.2025.112396
Zimeng Wang , Hao Li , Juan Li , Yachun Yang , Zuntao Xu , Jianbo Yang , Pengcheng Wei , Hui Ma
Rice (Oryza sativa L.) is one of the most important grain crops in the world. Abiotic stress such as low temperature is an important factor affecting the yield and quality of rice. To explore the endogenous stress-resistant genes and apply them to the breeding of new stress-resistant varieties is an effective way to improve the stress tolerance and adaptability of rice. PHD-finger transcription factor is a kind of zinc-finger structural protein that exists widely in eukaryotes. Its function is mainly focused on gene transcription and regulation of chromatin state, but there are few reports about its involvement in stress response. In the present study, a total of 58 PHD-finger transcription factors were identified, and two genes OsPHD13 and OsPHD52 were significantly up-regulated under low temperature stress. After low temperature induction, GUS driven by OsPHD13 and OsPHD52 promoters had different expression activities in roots, stems and leaves of transgenic plants. Further functional analysis of the pOsPHD13 and pOsPHD52 showed that each of them had a cis-acting element of CRT/DRE in response to low temperature stress. Both in yeast one-hybrid assays and in in vitro gel-shift analysis, CBF protein could specifically bind to the CRT/DRE element in the promoter.
{"title":"Identification and characterization of cold-responsive cis-element in the OsPHD13 and OsPHD52 promoter and its upstream regulatory proteins in rice","authors":"Zimeng Wang , Hao Li , Juan Li , Yachun Yang , Zuntao Xu , Jianbo Yang , Pengcheng Wei , Hui Ma","doi":"10.1016/j.plantsci.2025.112396","DOIUrl":"10.1016/j.plantsci.2025.112396","url":null,"abstract":"<div><div>Rice (<em>Oryza sativa</em> L.) is one of the most important grain crops in the world. Abiotic stress such as low temperature is an important factor affecting the yield and quality of rice. To explore the endogenous stress-resistant genes and apply them to the breeding of new stress-resistant varieties is an effective way to improve the stress tolerance and adaptability of rice. PHD-finger transcription factor is a kind of zinc-finger structural protein that exists widely in eukaryotes. Its function is mainly focused on gene transcription and regulation of chromatin state, but there are few reports about its involvement in stress response. In the present study, a total of 58 PHD-finger transcription factors were identified, and two genes <em>OsPHD13</em> and <em>OsPHD52</em> were significantly up-regulated under low temperature stress. After low temperature induction, <em>GUS</em> driven by <em>OsPHD13</em> and <em>OsPHD52</em> promoters had different expression activities in roots, stems and leaves of transgenic plants. Further functional analysis of the <em>pOsPHD13</em> and <em>pOsPHD52</em> showed that each of them had a cis-acting element of CRT/DRE in response to low temperature stress. Both in yeast one-hybrid assays and in in vitro gel-shift analysis, CBF protein could specifically bind to the CRT/DRE element in the promoter.</div></div>","PeriodicalId":20273,"journal":{"name":"Plant Science","volume":"352 ","pages":"Article 112396"},"PeriodicalIF":4.2,"publicationDate":"2025-01-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143029432","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Premature senescence has a significant impact on the yield and quality of wheat crops. The process is controlled by multiple and intricate genetic pathways and regulatory elements, whereby the discovery of additional mutants provides important insights into the molecular basis of this important trait. Here, we developed a premature senescence wheat mutant je0874, its leaves started to show yellow before heading stage; with plant growth and development, the degree of yellowing worsened rapidly, and chlorophyll content in flag leaf was reduced by 93.8 % at 15 days after heading, all other leaves became dryness at the grain filling stage. In the mutant, the reactive oxygen species (ROS) and its metabolites increased up to 34.8–47.3 %, while activities of ROS scavenging enzymes were reduced by 62.7–96.7 %. Premature senescence resulted in a reduction of thousand grain weight by over 50 %. Genetic analysis showed the mutation of senescence was controlled by a single recessive gene, and target gene was finely mapped to a 338 kb region of the long arm of chromosome 2D. This region contained a total of 6 annotated genes, while only gene TraesFLD2D01G513900 carried a SNP mutation. The gene contained an NBS-LRR domain, we named it Taps1. Allelic mutants of Taps1 exhibited a lesion mimic phenotype, and the mutant allele resulted in cell death in tobacco, which represent a novel gene controlling wheat senescence. Two haplotypes were identified in 180 accessions, which did not lead to cell death. These results contribute to increase our understanding of the regulation of premature plant senescence.
{"title":"The allelic mutation of NBS-LRR gene causes premature senescence in wheat","authors":"Lin Qiu , Rongmin Fang , Yong Jia , Hongchun Xiong , Yongdun Xie , Linshu Zhao , Jiayu GU , Shirong Zhao , Yuping Ding , Chengdao LI , Huijun Guo , Luxiang Liu","doi":"10.1016/j.plantsci.2025.112395","DOIUrl":"10.1016/j.plantsci.2025.112395","url":null,"abstract":"<div><div>Premature senescence has a significant impact on the yield and quality of wheat crops. The process is controlled by multiple and intricate genetic pathways and regulatory elements, whereby the discovery of additional mutants provides important insights into the molecular basis of this important trait. Here, we developed a premature senescence wheat mutant <em>je0874</em>, its leaves started to show yellow before heading stage; with plant growth and development, the degree of yellowing worsened rapidly, and chlorophyll content in flag leaf was reduced by 93.8 % at 15 days after heading, all other leaves became dryness at the grain filling stage. In the mutant, the reactive oxygen species (ROS) and its metabolites increased up to 34.8–47.3 %, while activities of ROS scavenging enzymes were reduced by 62.7–96.7 %. Premature senescence resulted in a reduction of thousand grain weight by over 50 %. Genetic analysis showed the mutation of senescence was controlled by a single recessive gene, and target gene was finely mapped to a 338 kb region of the long arm of chromosome 2D. This region contained a total of 6 annotated genes, while only gene <em>TraesFLD2D01G513900</em> carried a SNP mutation. The gene contained an NBS-LRR domain, we named it <em>Taps1</em>. Allelic mutants of <em>Taps1</em> exhibited a lesion mimic phenotype, and the mutant allele resulted in cell death in tobacco, which represent a novel gene controlling wheat senescence. Two haplotypes were identified in 180 accessions, which did not lead to cell death. These results contribute to increase our understanding of the regulation of premature plant senescence.</div></div>","PeriodicalId":20273,"journal":{"name":"Plant Science","volume":"352 ","pages":"Article 112395"},"PeriodicalIF":4.2,"publicationDate":"2025-01-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143024451","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2025-01-17DOI: 10.1016/j.plantsci.2025.112394
Di Wang , Kai Xu , Shoujun Chen , Lei Wang , Qiaojun Lou , Changsen Zhong , Yawen Wang , Tiemei Li , Huaxiang Cheng , Lijun Luo , Liang Chen
Agricultural production is severely affected by environmental stresses such as drought, and deep rooting is an important factor enhancing crop drought avoidance. H+-ATPases provide a transmembrane proton gradient and are thought to play a crucial role in plant growth and abiotic stress responses. However, their expression under abiotic stress and function on deep rooting is poorly understood in rice. In this study, the conserved domains, potential phosphorylation sites, and three-dimensional structures of ten Oryza sativa PM H+-ATPases (OSAs) were analyzed. Quantitative PCR analysis revealed different expression patterns of these OSA genes under hormone treatment conditions (e.g., abscisic acid) and abiotic stress conditions (e.g., drought and salt stress). Subcellular localization analysis revealed that most OSA proteins were localized to the cell membrane. Phenotype determination of OSA mutants indicated that the ratio of deep rooting (RDR) of both osa7 and osa8 mutants was significantly reduced compared to that of wild-type rice plants. Additionally, OSA haplotypes in 268 rice accessions were analyzed, and the haplotypes associated with RDR were identified. The present results provide valuable information on crucial domains, expression patterns, and functional identification of OSA paralogs to reveal their role in rice responses to abiotic stress.
{"title":"Stress-responsive plasma membrane H+-ATPases regulate deep rooting in rice","authors":"Di Wang , Kai Xu , Shoujun Chen , Lei Wang , Qiaojun Lou , Changsen Zhong , Yawen Wang , Tiemei Li , Huaxiang Cheng , Lijun Luo , Liang Chen","doi":"10.1016/j.plantsci.2025.112394","DOIUrl":"10.1016/j.plantsci.2025.112394","url":null,"abstract":"<div><div>Agricultural production is severely affected by environmental stresses such as drought, and deep rooting is an important factor enhancing crop drought avoidance. H<sup>+</sup>-ATPases provide a transmembrane proton gradient and are thought to play a crucial role in plant growth and abiotic stress responses. However, their expression under abiotic stress and function on deep rooting is poorly understood in rice. In this study, the conserved domains, potential phosphorylation sites, and three-dimensional structures of ten <em>Oryza sativa</em> PM H<sup>+</sup>-ATPases (OSAs) were analyzed. Quantitative PCR analysis revealed different expression patterns of these <em>OSA</em> genes under hormone treatment conditions (e.g., abscisic acid) and abiotic stress conditions (e.g., drought and salt stress). Subcellular localization analysis revealed that most OSA proteins were localized to the cell membrane. Phenotype determination of <em>OSA</em> mutants indicated that the ratio of deep rooting (RDR) of both <em>osa7</em> and <em>osa8</em> mutants was significantly reduced compared to that of wild-type rice plants. Additionally<em>, OSA</em> haplotypes in 268 rice accessions were analyzed, and the haplotypes associated with RDR were identified. The present results provide valuable information on crucial domains, expression patterns, and functional identification of OSA paralogs to reveal their role in rice responses to abiotic stress.</div></div>","PeriodicalId":20273,"journal":{"name":"Plant Science","volume":"352 ","pages":"Article 112394"},"PeriodicalIF":4.2,"publicationDate":"2025-01-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143010337","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2025-01-17DOI: 10.1016/j.plantsci.2025.112393
Chaïma Chammakhi , Marie Pacoud , Alexandre Boscari , Antoine Berger , Haythem Mhadhbi , Imène Gharbi , Renaud Brouquisse
Flooding induces hypoxia in plant tissues, impacting various physiological and biochemical processes. This study investigates the adaptive response of the roots and nitrogen-fixing nodules of Medicago truncatula in symbiosis with Sinorhizobium meliloti under short-term hypoxia caused by flooding. Four-week-old plants were subjected to flooding for 1–4 days. Physiological parameters as well as the expression of the senescence marker gene MtCP6 remained unchanged after 4 days of flooding, indicating no senescence onset. Hypoxia was evident from the first day, as indicated by the upregulation of hypoxia marker genes (MtADH, MtPDC, MtAlaAT, MtERF73). Nitrogen-fixing capacity was unaffected after 1 day but markedly decreased after 4 days, while energy state (ATP/ADP ratio) significantly decreased from 1 day and was more affected in nodules than in roots. Nitric oxide (NO) production increased in roots but decreased in nodules after prolonged flooding. Nitrate reductase (NR) activity and expression of genes associated with Phytoglobin-NO (Pgb-NO) respiration (MtNR1, MtNR2, MtPgb1.1) were upregulated, suggesting a role in maintaining energy metabolism under hypoxia, but the use of M. truncatula nr1 and nr2 mutants, impaired in nitrite production, indicated the involvement of these two genes in ATP regeneration during initial flooding response. The addition of sodium nitroprusside or tungstate revealed that Pgb-NO respiration contributes significantly to ATP regeneration in both roots and nodules under flooding. Altogether, these results highlight the importance of NR1 and Pgb1.1 in the hypoxic response of legume root systems and show that nodules are more sensitive than roots to hypoxia.
洪水引起植物组织缺氧，影响多种生理生化过程。本研究研究了与墨氏中华根瘤菌共生的根和固氮根瘤在洪水引起的短期缺氧条件下的适应性反应。四周大的植物被淹了1到4天。淹水4天后，生理参数及衰老标志基因MtCP6的表达均未发生变化，说明未发生衰老。缺氧标志基因（MtADH、MtPDC、MtAlaAT、MtERF73）的上调表明，从第一天起缺氧就很明显。固氮能力在第1天不受影响，但在第4天显著下降，能量状态（ATP/ADP比值）从第1天开始显著下降，且根瘤受影响大于根。长时间淹水后，根系中一氧化氮（NO）产量增加，而根瘤中一氧化氮（NO）产量下降。硝酸盐还原酶（NR）活性和与植物红蛋白- no （pgp - no）呼吸相关基因（MtNR1, MtNR2, MtPgb1.1）的表达上调，提示其在缺氧条件下维持能量代谢中起作用，但矮根霉nr1和nr2突变体在亚硝酸盐生产中受损，表明这两个基因参与了初始洪水反应中ATP的再生。硝普钠或钨酸钠的加入表明，Pgb-NO呼吸对淹水条件下根和根瘤的ATP再生都有显著的促进作用。综上所述，这些结果突出了NR1和Pgb1.1在豆科植物根系缺氧反应中的重要性，表明豆科植物根瘤比根对缺氧更敏感。
{"title":"Differential regulation of the “phytoglobin-nitric oxide respiration” in Medicago truncatula roots and nodules submitted to flooding","authors":"Chaïma Chammakhi , Marie Pacoud , Alexandre Boscari , Antoine Berger , Haythem Mhadhbi , Imène Gharbi , Renaud Brouquisse","doi":"10.1016/j.plantsci.2025.112393","DOIUrl":"10.1016/j.plantsci.2025.112393","url":null,"abstract":"<div><div>Flooding induces hypoxia in plant tissues, impacting various physiological and biochemical processes. This study investigates the adaptive response of the roots and nitrogen-fixing nodules of <em>Medicago truncatula</em> in symbiosis with <em>Sinorhizobium meliloti</em> under short-term hypoxia caused by flooding. Four-week-old plants were subjected to flooding for 1–4 days. Physiological parameters as well as the expression of the senescence marker gene <em>MtCP6</em> remained unchanged after 4 days of flooding, indicating no senescence onset. Hypoxia was evident from the first day, as indicated by the upregulation of hypoxia marker genes (<em>MtADH</em>, <em>MtPDC, MtAlaAT, MtERF73</em>). Nitrogen-fixing capacity was unaffected after 1 day but markedly decreased after 4 days, while energy state (ATP/ADP ratio) significantly decreased from 1 day and was more affected in nodules than in roots. Nitric oxide (NO) production increased in roots but decreased in nodules after prolonged flooding. Nitrate reductase (NR) activity and expression of genes associated with Phytoglobin-NO (Pgb-NO) respiration (<em>MtNR1, MtNR2, MtPgb1.1</em>) were upregulated, suggesting a role in maintaining energy metabolism under hypoxia, but the use of <em>M. truncatula nr1</em> and <em>nr2</em> mutants, impaired in nitrite production, indicated the involvement of these two genes in ATP regeneration during initial flooding response. The addition of sodium nitroprusside or tungstate revealed that Pgb-NO respiration contributes significantly to ATP regeneration in both roots and nodules under flooding. Altogether, these results highlight the importance of NR1 and Pgb1.1 in the hypoxic response of legume root systems and show that nodules are more sensitive than roots to hypoxia.</div></div>","PeriodicalId":20273,"journal":{"name":"Plant Science","volume":"352 ","pages":"Article 112393"},"PeriodicalIF":4.2,"publicationDate":"2025-01-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143010342","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2025-01-17DOI: 10.1016/j.plantsci.2025.112390
Washu Dev , Fahmida Sultana , Hongge Li , Daowu Hu , Zhen Peng , Shoupu He , Haobo Zhang , Muhammad Waqas , Xiaoli Geng , Xiongming Du
Cold stress has a huge impact on the growth and development of cotton, presenting a significant challenge to its productivity. Comprehending the complex molecular mechanisms that control the reaction to CS is necessary for developing tactics to improve cold tolerance in cotton. This review paper explores how cotton responds to cold stress by regulating gene expression, focusing on both activating and repressing specific genes. We investigate the essential roles that transcription factors and regulatory elements have in responding to cold stress and controlling gene expression to counteract the negative impacts of low temperatures. Through a comprehensive examination of new publications, we clarify the intricacies of transcriptional reprogramming induced by cold stress, emphasizing the connections between different regulatory elements and signaling pathways. Additionally, we investigate the consecutive effects of cold stress on cotton yield, highlighting the physiological and developmental disturbances resulting from extended periods of low temperatures. The knowledge obtained from this assessment allows for a more profound comprehension of the molecular mechanisms that regulate cold stress responses, suggesting potential paths for future research to enhance cold tolerance in cotton by utilizing targeted genetic modifications and biotechnological interventions.
{"title":"Molecular mechanisms of cold stress response in cotton: Transcriptional reprogramming and genetic strategies for tolerance","authors":"Washu Dev , Fahmida Sultana , Hongge Li , Daowu Hu , Zhen Peng , Shoupu He , Haobo Zhang , Muhammad Waqas , Xiaoli Geng , Xiongming Du","doi":"10.1016/j.plantsci.2025.112390","DOIUrl":"10.1016/j.plantsci.2025.112390","url":null,"abstract":"<div><div>Cold stress has a huge impact on the growth and development of cotton, presenting a significant challenge to its productivity. Comprehending the complex molecular mechanisms that control the reaction to CS is necessary for developing tactics to improve cold tolerance in cotton. This review paper explores how cotton responds to cold stress by regulating gene expression, focusing on both activating and repressing specific genes. We investigate the essential roles that transcription factors and regulatory elements have in responding to cold stress and controlling gene expression to counteract the negative impacts of low temperatures. Through a comprehensive examination of new publications, we clarify the intricacies of transcriptional reprogramming induced by cold stress, emphasizing the connections between different regulatory elements and signaling pathways. Additionally, we investigate the consecutive effects of cold stress on cotton yield, highlighting the physiological and developmental disturbances resulting from extended periods of low temperatures. The knowledge obtained from this assessment allows for a more profound comprehension of the molecular mechanisms that regulate cold stress responses, suggesting potential paths for future research to enhance cold tolerance in cotton by utilizing targeted genetic modifications and biotechnological interventions.</div></div>","PeriodicalId":20273,"journal":{"name":"Plant Science","volume":"352 ","pages":"Article 112390"},"PeriodicalIF":4.2,"publicationDate":"2025-01-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143010345","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2025-01-16DOI: 10.1016/j.plantsci.2025.112389
Huiying Chen , Jia Du , Yifan Wang , Kexin Chao , Zitong Wang , Shahid Ali , Houqing Zeng
Low phosphate (LP) availability significantly impacts crop yield and quality. PHOSPHATE STARVATION RESPONSE1 (PHR1) along with PHR1-like 1 (PHL1) act as a key transcriptional regulator in a plant's adaptive response to LP conditions. Abscisic acid (ABA) plays an important role in how plants respond to environmental stresses like salinity and drought. However, the involvement of PHR1 and PHL1 in ABA response and signalling mechanisms remains to be fully understood. Our findings reveal that PHR1 and PHR1/PHL1 knockout mutations enhance the responsiveness of seed germination, early seedling growth, and stomatal opening to ABA in Arabidopsis. Furthermore, these mutations increase sensitivity to combined LP and ABA stress. In contrast, overexpression of PHR1 or PHL1 reduces this sensitivity in Arabidopsis. Knockout mutations of PHR1 and PHR1/PHL1 also increase sensitivity to salt and osmotic stresses, as well as to combined LP and salinity/osmotic stress, while overexpression of PHR1 or PHL1 reduces their sensitivity in seed germination and early seedling development. Knockout mutations of SPX1 and SPX2, negative regulators of PHR1 and PHL1, decrease sensitivity to ABA and salt/osmotic stresses in Arabidopsis. A group of genes related to ABA metabolism and signalling is significantly affected by the knockout or overexpression of PHR1 and PHL1, with a large proportion of these genes containing PHR1 binding site (P1BS) in their promoters. Moreover, the ABA-sensitive phenotype of phr1 or phr1 phl1 mutants can be rescued by PHR1 homologs from chlorophyte algae, liverwort and rice, suggesting their conserved roles in ABA signalling. These results indicate that PHR1 and its homologs negatively regulate plant responses to ABA in seed germination and stomatal aperture. This study provides new insights into the interplay between Pi homeostasis, abiotic stress and ABA signaling. Moderately increasing the expression of PHR1 or its homologs in crops could be a potential strategy to enhance plant resistance to combined LP and osmotic stress.
{"title":"Transcription factors PHR1 and PHR1-like 1 regulate ABA-mediated inhibition of seed germination and stomatal opening in Arabidopsis","authors":"Huiying Chen , Jia Du , Yifan Wang , Kexin Chao , Zitong Wang , Shahid Ali , Houqing Zeng","doi":"10.1016/j.plantsci.2025.112389","DOIUrl":"10.1016/j.plantsci.2025.112389","url":null,"abstract":"<div><div>Low phosphate (LP) availability significantly impacts crop yield and quality. PHOSPHATE STARVATION RESPONSE1 (PHR1) along with PHR1-like 1 (PHL1) act as a key transcriptional regulator in a plant's adaptive response to LP conditions. Abscisic acid (ABA) plays an important role in how plants respond to environmental stresses like salinity and drought. However, the involvement of PHR1 and PHL1 in ABA response and signalling mechanisms remains to be fully understood. Our findings reveal that <em>PHR1</em> and <em>PHR1</em>/<em>PHL1</em> knockout mutations enhance the responsiveness of seed germination, early seedling growth, and stomatal opening to ABA in <em>Arabidopsis</em>. Furthermore, these mutations increase sensitivity to combined LP and ABA stress. In contrast, overexpression of <em>PHR1</em> or <em>PHL1</em> reduces this sensitivity in <em>Arabidopsis</em>. Knockout mutations of <em>PHR1</em> and <em>PHR1</em>/<em>PHL1</em> also increase sensitivity to salt and osmotic stresses, as well as to combined LP and salinity/osmotic stress, while overexpression of <em>PHR1</em> or <em>PHL1</em> reduces their sensitivity in seed germination and early seedling development. Knockout mutations of <em>SPX1</em> and <em>SPX2</em>, negative regulators of PHR1 and PHL1, decrease sensitivity to ABA and salt/osmotic stresses in <em>Arabidopsis</em>. A group of genes related to ABA metabolism and signalling is significantly affected by the knockout or overexpression of <em>PHR1</em> and <em>PHL1</em>, with a large proportion of these genes containing PHR1 binding site (P1BS) in their promoters. Moreover, the ABA-sensitive phenotype of <em>phr1</em> or <em>phr1 phl1</em> mutants can be rescued by PHR1 homologs from chlorophyte algae, liverwort and rice, suggesting their conserved roles in ABA signalling. These results indicate that PHR1 and its homologs negatively regulate plant responses to ABA in seed germination and stomatal aperture. This study provides new insights into the interplay between Pi homeostasis, abiotic stress and ABA signaling. Moderately increasing the expression of <em>PHR1</em> or its homologs in crops could be a potential strategy to enhance plant resistance to combined LP and osmotic stress.</div></div>","PeriodicalId":20273,"journal":{"name":"Plant Science","volume":"352 ","pages":"Article 112389"},"PeriodicalIF":4.2,"publicationDate":"2025-01-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143010340","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2025-01-13DOI: 10.1016/j.plantsci.2025.112388
Shipeng Zhang , Dengyu Zheng , Yuqi Gao , Meng She , Zhongyi Wu , Yuncai Lu , Zhongbao Zhang
The JAZ protein family, serving as a key negative regulator in the jasmonic acid signaling pathway, interacts with transcription factors to play an essential role in plant growth, development, and stress responses. However, minimal research has focused on the role of JAZ transcription factors in regulating the growth, development, and stress responses of maize. In this study, we cloned the JAZ gene ZmJAZ13 from maize (Zea mays L.) and conducted a preliminary analysis of its biological function. ZmJAZ13 was highly expressed in maize immature embryos and was induced by abiotic stress and plant hormone treatments. Y2H and BiFC assays revealed interactions between ZmJAZ13 and ZmbHLH161, as well as ZmA0A1D6GLB9. Heterologous expression of ZmJAZ13 in Arabidopsis significantly enhanced plant tolerance to drought and salt stress, increased chlorophyll content, decreased malondialdehyde content, and enhanced peroxidase activity. Under abiotic stress, heterologous expression of ZmJAZ13 in Arabidopsis upregulated the expression levels of stress-related genes (RD22, RD29-A). Together, these results suggested that ZmJAZ13 may respond to abiotic stress, providing a foundation for further investigation into the mechanism of action of ZmJAZ13 in maize.
{"title":"The TIFY transcription factor ZmJAZ13 enhances plant tolerance to drought and salt stress by interacting with ZmbHLH161 and ZmA0A1D6GLB9","authors":"Shipeng Zhang , Dengyu Zheng , Yuqi Gao , Meng She , Zhongyi Wu , Yuncai Lu , Zhongbao Zhang","doi":"10.1016/j.plantsci.2025.112388","DOIUrl":"10.1016/j.plantsci.2025.112388","url":null,"abstract":"<div><div>The JAZ protein family, serving as a key negative regulator in the jasmonic acid signaling pathway, interacts with transcription factors to play an essential role in plant growth, development, and stress responses. However, minimal research has focused on the role of JAZ transcription factors in regulating the growth, development, and stress responses of maize. In this study, we cloned the JAZ gene <em>ZmJAZ13</em> from maize (<em>Zea mays</em> L.) and conducted a preliminary analysis of its biological function. <em>ZmJAZ13</em> was highly expressed in maize immature embryos and was induced by abiotic stress and plant hormone treatments. Y2H and BiFC assays revealed interactions between <em>ZmJAZ13</em> and <em>ZmbHLH161</em>, as well as <em>ZmA0A1D6GLB9</em>. Heterologous expression of <em>ZmJAZ13</em> in <em>Arabidopsis</em> significantly enhanced plant tolerance to drought and salt stress, increased chlorophyll content, decreased malondialdehyde content, and enhanced peroxidase activity. Under abiotic stress, heterologous expression of <em>ZmJAZ13</em> in <em>Arabidopsis</em> upregulated the expression levels of stress-related genes (<em>RD22</em>, <em>RD29-A</em>). Together, these results suggested that <em>ZmJAZ13</em> may respond to abiotic stress, providing a foundation for further investigation into the mechanism of action of <em>ZmJAZ13</em> in maize.</div></div>","PeriodicalId":20273,"journal":{"name":"Plant Science","volume":"352 ","pages":"Article 112388"},"PeriodicalIF":4.2,"publicationDate":"2025-01-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143010339","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2025-01-12DOI: 10.1016/j.plantsci.2025.112392
Gaofeng Zhang , Weichao Li , Tong Han , Tianyi Huang , Lirong Sun , Fushun Hao
Tryptophan-arginine-lysine-tyrosine (WRKY) transcription factors are essential regulators of drought tolerance in multiple plants. However, whether and how GhWRKY207 modulates cotton response to drought stress is unclear. In this study, we determined that GhWRKY207 expression was high in leaves and induced by drought stress. The gene encoded a nuclear protein that had transcriptional activation activity. Silencing GhWRKY207 by virus-induced gene silencing (VIGS) caused significant reduction in drought tolerance of cotton plants. Consistently, overexpression of GhWRKY207 in Arabidopsis thaliana wild type (WT) plants clearly enhanced their drought tolerance. Moreover, GhWRKY207 VIGS plants had notably increased malondialdehyde (MDA) contents, electrolyte leakage percentages and O2·− accumulation rates whereas GhWRKY207 overexpression lines showed markedly decreased levels of the three parameters compared to their corresponding controls under water deficit conditions. Additionally, GhWRKY207 enhanced superoxide dismutase (SOD) activity by directly activating the expression of GhCu/Zn-SOD3 (GhCSD3) and GhFe-SOD2 (GhFSD2) genes. Silencing GhCSD3 or GhFSD2 also markedly reduced drought tolerance of cotton plants. Taken together, these results suggest that GhWRKY207 positively regulates drought tolerance by inducing the expression of GhCSD3 and GhFSD2 in Gossypium hirsutum.
{"title":"GhWRKY207 improves drought tolerance through promoting the expression of GhCSD3 and GhFSD2 in Gossypium hirsutum","authors":"Gaofeng Zhang , Weichao Li , Tong Han , Tianyi Huang , Lirong Sun , Fushun Hao","doi":"10.1016/j.plantsci.2025.112392","DOIUrl":"10.1016/j.plantsci.2025.112392","url":null,"abstract":"<div><div>Tryptophan-arginine-lysine-tyrosine (WRKY) transcription factors are essential regulators of drought tolerance in multiple plants. However, whether and how GhWRKY207 modulates cotton response to drought stress is unclear. In this study, we determined that <em>GhWRKY207</em> expression was high in leaves and induced by drought stress. The gene encoded a nuclear protein that had transcriptional activation activity. Silencing <em>GhWRKY207</em> by virus-induced gene silencing (VIGS) caused significant reduction in drought tolerance of cotton plants. Consistently, overexpression of <em>GhWRKY207</em> in <em>Arabidopsis thaliana</em> wild type (WT) plants clearly enhanced their drought tolerance. Moreover, <em>GhWRKY207</em> VIGS plants had notably increased malondialdehyde (MDA) contents, electrolyte leakage percentages and O<sub>2</sub><sup><strong>·−</strong></sup> accumulation rates whereas <em>GhWRKY207</em> overexpression lines showed markedly decreased levels of the three parameters compared to their corresponding controls under water deficit conditions. Additionally, GhWRKY207 enhanced superoxide dismutase (SOD) activity by directly activating the expression of <em>GhCu/Zn-SOD3</em> (<em>GhCSD3</em>) and <em>GhFe-SOD2</em> (<em>GhFSD2</em>) genes. Silencing <em>GhCSD3</em> or <em>GhFSD2</em> also markedly reduced drought tolerance of cotton plants. Taken together, these results suggest that GhWRKY207 positively regulates drought tolerance by inducing the expression of <em>GhCSD3</em> and <em>GhFSD2</em> in <em>Gossypium hirsutum</em>.</div></div>","PeriodicalId":20273,"journal":{"name":"Plant Science","volume":"352 ","pages":"Article 112392"},"PeriodicalIF":4.2,"publicationDate":"2025-01-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142984356","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2025-01-12DOI: 10.1016/j.plantsci.2025.112391
Wenying Wei, Zesheng Liu, Xuejuan Pan, Tingyue Yang, Caiting An, Yuanhui Wang, Long Li, Weibiao Liao, Chunlei Wang
Reactive oxygen species (ROS) serve as important signaling molecule, involved in numerous biological processes, particularly in the physiological changes associated with fruit ripening and postharvest handing. This review explores ROS key role in plant fruit ripening and postharvest quality. The mechanism of ROS production and degradation in maintaining ROS homeostasis are analyzed in detail. Fruit ripening is a complex and highly coordinated process involving physiological and biochemical changes. Studies have observed that the content of ROS, mainly hydrogen peroxide (H2O2), dynamically changes in various types of fruits during ripening. Furthermore, ROS have significant effects on fruit softening, color change, and other ripening processes. In addition, in the postharvest stage, the abnormal accumulation of ROS isclosely related to the decline in fruit quality and the occurrence of decay browning, which seriously affects the market value and shelf life of fruit. Overall, this review demonstrates the crucial role of ROS in regulating the ripening process and postharvest quality of fruit.
{"title":"Effects of reactive oxygen species on fruit ripening and postharvest fruit quality","authors":"Wenying Wei, Zesheng Liu, Xuejuan Pan, Tingyue Yang, Caiting An, Yuanhui Wang, Long Li, Weibiao Liao, Chunlei Wang","doi":"10.1016/j.plantsci.2025.112391","DOIUrl":"10.1016/j.plantsci.2025.112391","url":null,"abstract":"<div><div>Reactive oxygen species (ROS) serve as important signaling molecule, involved in numerous biological processes, particularly in the physiological changes associated with fruit ripening and postharvest handing. This review explores ROS key role in plant fruit ripening and postharvest quality. The mechanism of ROS production and degradation in maintaining ROS homeostasis are analyzed in detail. Fruit ripening is a complex and highly coordinated process involving physiological and biochemical changes. Studies have observed that the content of ROS, mainly hydrogen peroxide (H<sub>2</sub>O<sub>2</sub>), dynamically changes in various types of fruits during ripening. Furthermore, ROS have significant effects on fruit softening, color change, and other ripening processes. In addition, in the postharvest stage, the abnormal accumulation of ROS isclosely related to the decline in fruit quality and the occurrence of decay browning, which seriously affects the market value and shelf life of fruit. Overall, this review demonstrates the crucial role of ROS in regulating the ripening process and postharvest quality of fruit.</div></div>","PeriodicalId":20273,"journal":{"name":"Plant Science","volume":"352 ","pages":"Article 112391"},"PeriodicalIF":4.2,"publicationDate":"2025-01-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142979739","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
扫码关注我们
求助内容：
应助结果提醒方式：
京公网安备 11010802042870号